| General information | |||
| TC033134
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 9407291..9415214 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC033134 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_10056 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC033134 RA
MQGFGFVTF ANSADAERA RDRLHGTVV EGRKIEVNN ATARVQTKK PPTLPTVPV IQWSDATLR AGVAAIQRG RARAAYPAA AAAAAAAFA RHPTPLAGA LHGYTPVLN TLPVDGSVY YDPFLAAHA ADPNYRLQA AAAATPLLK TPLSTAQQA SYAAAATYT AVAARYGAA QPVAGYAAV AGYGRDYAD PYLGHGIGP MAGYGAAVY RSGYNRFTP Y
- >TC033134 RB
MLKHFTILT ESNIVNNAT ARVQTKKPP TLPTVPVIQ WSDATLRAG VAAIQRGRA RAAYPAAAA AAAAAFARH PTPLAGALH GYTPVLNTL PVDGSVYYD PFLAAHAAD PNYRLQAAA AATPLLKTP LSTAQQASY AAAATYTAV AARYGAAQP VAGYAAVAG YGRDYADPY LGHGIGPMA GYGAAVYRS GYNRFTPY
- >TC033134 RA
atgcagggg ttcggtttt gtaacattc gcaaatagt gctgatgcg gagcgcgca cgagaccga ctacacggc accgtggtt gagggaaga aaaatagag gtcaataat gcaactgca agagtacaa actaagaaa ccaccaacg cttccaaca gttccggta atacaatgg tccgatgcc acactgcga gctggagtg gcggccatt cagagagga cgagcaagg gcagcatat ccggcagcc gctgcagca gctgcagcc gcatttgcg aggcatccg acgcctttg gctggagct ttacacggt tacaccccc gttttgaac acattacct gtagatggc agcgtttac tatgaccct tttctggct gcccacgca gctgatccc aattataga ctacaggct gctgcggcc gcaacacct ctattaaaa acacctctg tcgactgcc caacaggcg agttacgct gccgctgcc acctatacg gcagttgcg gcgcgttac ggagccgct caacccgtc gccggttac gccgctgtc gccggttac ggccgggat tacgctgat ccctacctg ggacacggg atcgggccg atggccgga tatggggct gctgtgtat cggagcggt tataaccgg ttcacgccc tattga
- >TC033134 RB
atgctaaaa cactttacc atccttact gaatctaat atagtcaat aatgcaact gcaagagta caaactaag aaaccacca acgcttcca acagttccg gtaatacaa tggtccgat gccacactg cgagctgga gtggcggcc attcagaga ggacgagca agggcagca tatccggca gccgctgca gcagctgca gccgcattt gcgaggcat ccgacgcct ttggctgga gctttacac ggttacacc cccgttttg aacacatta cctgtagat ggcagcgtt tactatgac ccttttctg gctgcccac gcagctgat cccaattat agactacag gctgctgcg gccgcaaca cctctatta aaaacacct ctgtcgact gcccaacag gcgagttac gctgccgct gccacctat acggcagtt gcggcgcgt tacggagcc gctcaaccc gtcgccggt tacgccgct gtcgccggt tacggccgg gattacgct gatccctac ctgggacac gggatcggg ccgatggcc ggatatggg gctgctgtg tatcggagc ggttataac cggttcacg ccctattga
- >TC033134 RA
taatagcag ttaatttcc tgagaaata tttgatatg aattattta tctctacta atttatttt tgctaaagt ctggaagaa gtgcatgat aatttcgtt aacacagtt ttattttta cacaaagag aataacttt agttctcat ataatagtc agtgtgttt ggatttatt gtctggctt tgacgcatg aacttgtag taattgaat ttactgaaa tgaacgttt tgaaatatt agttcgaca aggagatga caatgaaga gcgaaagag aataagact tgcagttac tggatttgt tttatgaac aagaaatgg caatggaag aggcgaatg catgggata actctttga gttgttttc ctaataatt aatgttttg tggttggca caataaata gaatgatgt atgggcata ttgcacttg tctatgggc tttttcagt gaataacac cctggtaaa taacacctt agatttgat agcagcaaa gctgagtag gctctttca cacgagaaa tttgacatc gcagtgtaa ttattatat gatgcatgt tatttacta taaatacaa catacatta tcttatata caaatatcg ttcaagtgc aatttgatg acttttact aaacatatg acatatgat ggcgtagtg agatttggt ttttttcat ataatgatt gtgctgtag cagctattt gagttcgtc tttgatttg acatgatct gtccgatct tcatatcca tttacttaa aaatgattc ggggcgatt tctgcaggt ttaacacta aacaattgg attttacgt ttgtcattc ctgcagttg atcacctga aaagtaaag agtgcccca gtgcaggct cgattctgt tagttcctt attgccttg tcagtttcc cgtactgtt tgtattctg ttctgtgtt ggttttcaa cgggggctt tgagttgtg tgttctagc tttggaatt ttgttacct tgctcactg agaaacgaa ccgattaag gggttacat gcctaataa tccacaagt agttgaaca taacagcaa cttcattac gtgtgtgtg tctaacagt cgccttaga tcagtaata attaccaca ctagctgta acagtgcta agagcgagc tagagcgga ggtcggcaa caacactca catccgtgt atttcgcct gcacaaaca cccacaccg gcaaagaaa atacagtca attttgttg tagttttat gtgtattaa cagagataa tagtgtgga gtggcaagg ttcccttta gcttgctct tagctttct agttcgctt aggtgaaga ggagtaagg agaagcggc gtgtagtga atttgataa ggagaagcg tggagttgg aagcgacga aattgaatt gcaatgaag gaaattgcc atccaggcg aaggaaatt gcgtttgtc gtctttctc agtgacgaa ggtgcacaa aaccgatac acacagctc aaagtggtc gtggagcgc gagagaaag tgtcgcctc ctcgccgcg ttgatcggt ggaggcggc gtcgagtag atggatatg agcagagag gcggcgagt ggtagtgta catagctag gcgcactgg cgcactccc gtcacgtgg agcgaatgc cgccgccga cttgaggct taacagtgt aaagtagtc taacccgag agccgagac tagtgagcg agtgtggaa tgaggcttc tagacagaa gcatgtcta atgtctaac gtcatcatg tcattgcac gagtttgaa gagctgttt ataatgcat gtcttgtct gtctctgca tgcaggggt tcggttttg taacattcg caaatagtg ctgatgcgg agcgcgcac gagaccgac tacacggca ccgtggttg agggaagaa aaatagagg tcaataatg caactgcaa gagtacaaa ctaagaaac caccaacgc ttccaacag ttccggtaa tacaatggt ccgatgcca cactgcgag ctggagtgg cggccattc agagaggac gagcaaggg cagcatatc cggcagccg ctgcagcag ctgcagccg catttgcga ggcatccga cgcctttgg ctggagctt tacacggtt acacccccg ttttgaaca cattacctg tagatggca gcgtttact atgaccctt ttctggctg cccacgcag ctgatccca attatagac tacaggctg ctgcggccg caacacctc tattaaaaa cacctctgt cgactgccc aacaggcga gttacgctg ccgctgcca cctatacgg cagttgcgg cgcgttacg gagccgctc aacccgtcg ccggttacg ccgctgtcg ccggttacg gccgggatt acgctgatc cctacctgg gacacggga tcgggccga tggccggat atggggctg ctgtgtatc ggagcggtt ataaccggt tcacgccct attgaacat atccgcagc tccgttagg cctacgaaa atactcaac tgttatttg tcttgtgta cgtagcaca aaacagcac aaacaagaa tgagccaaa gagctgtaa ctcactaac gtaagccct tgtgatagc ccacctaac ccagctctc taacttagt taaggatta gtcataagg tccgtttcc attcactta tacggctac cttttactc gaaactgac tcaggtcgg ttggaacgg taagtgagg atagttgta gggtattgc aggacgtgt agtgttacg tactttatt ttaacttaa gacatgtga cacaaatgc catgaccag gataattag ctatcgttc agactgtta ttaagtacc ttactcagt cacagaaag gcttgtgtt aggaatcat taggcgata aagcaataa gttcaattt ttcgttcga tcgctgttt taatgactg gaaactgaa caatatcat gcggtattc gattttaaa accacattg tacacatag atacatatg tcaaatagt aggttgtac gatctttaa aaactacta aaaatttta cccttttat cgattaaga ttttgacag gtttaatcg tggctgttc ttagttgtg gacaaatag tagttccga cttccacta tcgtcaagt ttgaactca acgtgaatt tttcttaat tttggacaa tagtggaag ccccataat ggtgaaaat tcgatataa tgacacatt tgtcttgta gttgtaaga tttaagtta taaaatgac tggtaggac ttacattac gctctctcg agtctcatt tgtatttat gaatagtta agtttattc gatttttag tataagtgc aatggaaac taatttccc acgtgttta gtgtatttt tagttagtt atattttga tactctatt cttttttaa gcagatgtt gtaggtctg catgacata tcgacgata atctctcta tattatagc tctctagaa tgtacagtt ttttcaaag atctgtttt taattgtgt aaaccctgg gatgtgttt tcgcatttg ccatctgta aattttggt ttgatttat tcagttttg ttgatttcc tgtgttgca acgcacaaa atgctttcc gtgagtgtg aaaatacat ccttcaaat taaggccaa taagctcta gtctctgta aatatttca tacgtacaa aacccatcc tcttttggt tacgatccc aagaatttt cggttttta tcagtatca tcccatttt attataata acccccaat atattcata cctttttat gaataaaac gtaaaagag gtacaagca caaaggctg cggtatttt atttattga ccccgtcat cttaaaaaa cccgtgaat aattgtgaa aaaatcgg
- >TC033134 RB
tctttagac ccaaaattt tgtcttcaa caacaccac ctgattgta atttttttg gcacaaaaa ttcttatta caaaactgt tctaaaatc taggccaga tccgagttt ttaccataa acaaagtct attattggc ttattttaa tatctaact ttttgtagt gggatttgg tcacaatct gcatttgat aaattaatg tttcattat tacatacga tagatttat tcttaaaac taaacttga gactaaaac ttttttgaa ttagctttt gtattttgt tcgttgttt atacaatat aatcgaatt taacttgtt tagtgtaaa tgtagtagt cataataat tataaaaga aaacatgca caagtgata aggaaatga agatttttt ggtttattg gttgttgtt gggtttata ttaattagt ttaggtaat aaaagagga agagaaatt atattcaga gtcaagtct ttgtgagat ttattatta tgtaaattt tcaaataag atttcaatg ttttggcaa gttttcaac taaaaatta acttagttt attttgttt ttaaattga tttatgaaa ctttcttga agttataaa acattccac aaaacagta gtgattaag taagtgttg ttaagagat ttcatatta ctggcccac agtagataa aatatttat attaattaa tgaacaatc aaggcctgg taatgtgag atttttcca tagaaatgc taaaacact ttaccatcc ttactgaat ctaatatag tcaataatg caactgcaa gagtacaaa ctaagaaac caccaacgc ttccaacag ttccggtaa tacaatggt ccgatgcca cactgcgag ctggagtgg cggccattc agagaggac gagcaaggg cagcatatc cggcagccg ctgcagcag ctgcagccg catttgcga ggcatccga cgcctttgg ctggagctt tacacggtt acacccccg ttttgaaca cattacctg tagatggca gcgtttact atgaccctt ttctggctg cccacgcag ctgatccca attatagac tacaggctg ctgcggccg caacacctc tattaaaaa cacctctgt cgactgccc aacaggcga gttacgctg ccgctgcca cctatacgg cagttgcgg cgcgttacg gagccgctc aacccgtcg ccggttacg ccgctgtcg ccggttacg gccgggatt acgctgatc cctacctgg gacacggga tcgggccga tggccggat atggggctg ctgtgtatc ggagcggtt ataaccggt tcacgccct attgaacat atccgcagc tccgttagg cctacgaaa atactcaac tgttatttg tcttgtgta cgtagcaca aaacagcac aaacaagaa tgagccaaa gagctgtaa ctcactaac gtaagccct tgtgatagc ccacctaac ccagctctc taacttagt taaggatta gtcataagg tccgtttcc attcactta tacggctac cttttactc gaaactgac tcaggtcgg ttggaacgg taagtgagg atagttgta gggtattgc aggacgtgt agtgttacg tactttatt ttaacttaa gacatgtga cacaaatgc catgaccag gataattag ctatcgttc agactgtta ttaagtacc ttactcagt cacagaaag gcttgtgtt aggaatcat taggcgata aagcaataa gttcaattt ttcgttcga tcgctgttt taatgactg gaaactgaa caatatcat gcggtattc gattttaaa accacattg tacacatag atacatatg tcaaatagt aggttgtac gatctttaa aaactacta aaaatttta cccttttat cgattaaga ttttgacag gtttaatcg tggctgttc ttagttgtg gacaaatag tagttccga cttccacta tcgtcaagt ttgaactca acgtgaatt tttcttaat tttggacaa tagtggaag ccccataat ggtgaaaat tcgatataa tgacacatt tgtcttgta gttgtaaga tttaagtta taaaatgac tggtaggac ttacattac gctctctcg agtctcatt tgtatttat gaatagtta agtttattc gatttttag tataagtgc aatggaaac taatttccc acgtgttta gtgtatttt tagttagtt atattttga tactctatt cttttttaa gcagatgtt gtaggtctg catgacata tcgacgata atctctcta tattatagc tctctagaa tgtacagtt ttttcaaag atctgtttt taattgtgt aaaccctgg gatgtgttt tcgcatttg ccatctgta aattttggt ttgatttat tcagttttg ttgatttcc tgtgttgca acgcacaaa atgctttcc gtgagtgtg aaaatacat ccttcaaat taaggccaa taagctcta gtctctgta aatatttca tacgtacaa aacccatcc tcttttggt tacgatccc aagaatttt cggttttta tcagtatca tcccatttt attataata acccccaat atattcata cctttttat gaataaaac gtaaaagag gtacaagca caaaggctg cggtatttt atttattga ccccgtcat cttaaaaaa cccgtgaat aattgtgaa aaaatcgg
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
