Status
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Gene information
General information
TC033134
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC030589
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9407291..9415214
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033134
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10056

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033134 RA
      MQGFGFVTF ANSADAERA RDRLHGTVV 
      EGRKIEVNN ATARVQTKK PPTLPTVPV 
      IQWSDATLR AGVAAIQRG RARAAYPAA 
      AAAAAAAFA RHPTPLAGA LHGYTPVLN 
      TLPVDGSVY YDPFLAAHA ADPNYRLQA 
      AAAATPLLK TPLSTAQQA SYAAAATYT 
      AVAARYGAA QPVAGYAAV AGYGRDYAD 
      PYLGHGIGP MAGYGAAVY RSGYNRFTP 
      Y
    • >TC033134 RB
      MLKHFTILT ESNIVNNAT ARVQTKKPP 
      TLPTVPVIQ WSDATLRAG VAAIQRGRA 
      RAAYPAAAA AAAAAFARH PTPLAGALH 
      GYTPVLNTL PVDGSVYYD PFLAAHAAD 
      PNYRLQAAA AATPLLKTP LSTAQQASY 
      AAAATYTAV AARYGAAQP VAGYAAVAG 
      YGRDYADPY LGHGIGPMA GYGAAVYRS 
      GYNRFTPY
    CDS sequence
    • >TC033134 RA
      atgcagggg ttcggtttt gtaacattc gcaaatagt 
      gctgatgcg gagcgcgca cgagaccga ctacacggc 
      accgtggtt gagggaaga aaaatagag gtcaataat 
      gcaactgca agagtacaa actaagaaa ccaccaacg 
      cttccaaca gttccggta atacaatgg tccgatgcc 
      acactgcga gctggagtg gcggccatt cagagagga 
      cgagcaagg gcagcatat ccggcagcc gctgcagca 
      gctgcagcc gcatttgcg aggcatccg acgcctttg 
      gctggagct ttacacggt tacaccccc gttttgaac 
      acattacct gtagatggc agcgtttac tatgaccct 
      tttctggct gcccacgca gctgatccc aattataga 
      ctacaggct gctgcggcc gcaacacct ctattaaaa 
      acacctctg tcgactgcc caacaggcg agttacgct 
      gccgctgcc acctatacg gcagttgcg gcgcgttac 
      ggagccgct caacccgtc gccggttac gccgctgtc 
      gccggttac ggccgggat tacgctgat ccctacctg 
      ggacacggg atcgggccg atggccgga tatggggct 
      gctgtgtat cggagcggt tataaccgg ttcacgccc 
      tattga
    • >TC033134 RB
      atgctaaaa cactttacc atccttact gaatctaat 
      atagtcaat aatgcaact gcaagagta caaactaag 
      aaaccacca acgcttcca acagttccg gtaatacaa 
      tggtccgat gccacactg cgagctgga gtggcggcc 
      attcagaga ggacgagca agggcagca tatccggca 
      gccgctgca gcagctgca gccgcattt gcgaggcat 
      ccgacgcct ttggctgga gctttacac ggttacacc 
      cccgttttg aacacatta cctgtagat ggcagcgtt 
      tactatgac ccttttctg gctgcccac gcagctgat 
      cccaattat agactacag gctgctgcg gccgcaaca 
      cctctatta aaaacacct ctgtcgact gcccaacag 
      gcgagttac gctgccgct gccacctat acggcagtt 
      gcggcgcgt tacggagcc gctcaaccc gtcgccggt 
      tacgccgct gtcgccggt tacggccgg gattacgct 
      gatccctac ctgggacac gggatcggg ccgatggcc 
      ggatatggg gctgctgtg tatcggagc ggttataac 
      cggttcacg ccctattga
    mRNA sequence
    • >TC033134 RA
      taatagcag ttaatttcc tgagaaata tttgatatg 
      aattattta tctctacta atttatttt tgctaaagt 
      ctggaagaa gtgcatgat aatttcgtt aacacagtt 
      ttattttta cacaaagag aataacttt agttctcat 
      ataatagtc agtgtgttt ggatttatt gtctggctt 
      tgacgcatg aacttgtag taattgaat ttactgaaa 
      tgaacgttt tgaaatatt agttcgaca aggagatga 
      caatgaaga gcgaaagag aataagact tgcagttac 
      tggatttgt tttatgaac aagaaatgg caatggaag 
      aggcgaatg catgggata actctttga gttgttttc 
      ctaataatt aatgttttg tggttggca caataaata 
      gaatgatgt atgggcata ttgcacttg tctatgggc 
      tttttcagt gaataacac cctggtaaa taacacctt 
      agatttgat agcagcaaa gctgagtag gctctttca 
      cacgagaaa tttgacatc gcagtgtaa ttattatat 
      gatgcatgt tatttacta taaatacaa catacatta 
      tcttatata caaatatcg ttcaagtgc aatttgatg 
      acttttact aaacatatg acatatgat ggcgtagtg 
      agatttggt ttttttcat ataatgatt gtgctgtag 
      cagctattt gagttcgtc tttgatttg acatgatct 
      gtccgatct tcatatcca tttacttaa aaatgattc 
      ggggcgatt tctgcaggt ttaacacta aacaattgg 
      attttacgt ttgtcattc ctgcagttg atcacctga 
      aaagtaaag agtgcccca gtgcaggct cgattctgt 
      tagttcctt attgccttg tcagtttcc cgtactgtt 
      tgtattctg ttctgtgtt ggttttcaa cgggggctt 
      tgagttgtg tgttctagc tttggaatt ttgttacct 
      tgctcactg agaaacgaa ccgattaag gggttacat 
      gcctaataa tccacaagt agttgaaca taacagcaa 
      cttcattac gtgtgtgtg tctaacagt cgccttaga 
      tcagtaata attaccaca ctagctgta acagtgcta 
      agagcgagc tagagcgga ggtcggcaa caacactca 
      catccgtgt atttcgcct gcacaaaca cccacaccg 
      gcaaagaaa atacagtca attttgttg tagttttat 
      gtgtattaa cagagataa tagtgtgga gtggcaagg 
      ttcccttta gcttgctct tagctttct agttcgctt 
      aggtgaaga ggagtaagg agaagcggc gtgtagtga 
      atttgataa ggagaagcg tggagttgg aagcgacga 
      aattgaatt gcaatgaag gaaattgcc atccaggcg 
      aaggaaatt gcgtttgtc gtctttctc agtgacgaa 
      ggtgcacaa aaccgatac acacagctc aaagtggtc 
      gtggagcgc gagagaaag tgtcgcctc ctcgccgcg 
      ttgatcggt ggaggcggc gtcgagtag atggatatg 
      agcagagag gcggcgagt ggtagtgta catagctag 
      gcgcactgg cgcactccc gtcacgtgg agcgaatgc 
      cgccgccga cttgaggct taacagtgt aaagtagtc 
      taacccgag agccgagac tagtgagcg agtgtggaa 
      tgaggcttc tagacagaa gcatgtcta atgtctaac 
      gtcatcatg tcattgcac gagtttgaa gagctgttt 
      ataatgcat gtcttgtct gtctctgca tgcaggggt 
      tcggttttg taacattcg caaatagtg ctgatgcgg 
      agcgcgcac gagaccgac tacacggca ccgtggttg 
      agggaagaa aaatagagg tcaataatg caactgcaa 
      gagtacaaa ctaagaaac caccaacgc ttccaacag 
      ttccggtaa tacaatggt ccgatgcca cactgcgag 
      ctggagtgg cggccattc agagaggac gagcaaggg 
      cagcatatc cggcagccg ctgcagcag ctgcagccg 
      catttgcga ggcatccga cgcctttgg ctggagctt 
      tacacggtt acacccccg ttttgaaca cattacctg 
      tagatggca gcgtttact atgaccctt ttctggctg 
      cccacgcag ctgatccca attatagac tacaggctg 
      ctgcggccg caacacctc tattaaaaa cacctctgt 
      cgactgccc aacaggcga gttacgctg ccgctgcca 
      cctatacgg cagttgcgg cgcgttacg gagccgctc 
      aacccgtcg ccggttacg ccgctgtcg ccggttacg 
      gccgggatt acgctgatc cctacctgg gacacggga 
      tcgggccga tggccggat atggggctg ctgtgtatc 
      ggagcggtt ataaccggt tcacgccct attgaacat 
      atccgcagc tccgttagg cctacgaaa atactcaac 
      tgttatttg tcttgtgta cgtagcaca aaacagcac 
      aaacaagaa tgagccaaa gagctgtaa ctcactaac 
      gtaagccct tgtgatagc ccacctaac ccagctctc 
      taacttagt taaggatta gtcataagg tccgtttcc 
      attcactta tacggctac cttttactc gaaactgac 
      tcaggtcgg ttggaacgg taagtgagg atagttgta 
      gggtattgc aggacgtgt agtgttacg tactttatt 
      ttaacttaa gacatgtga cacaaatgc catgaccag 
      gataattag ctatcgttc agactgtta ttaagtacc 
      ttactcagt cacagaaag gcttgtgtt aggaatcat 
      taggcgata aagcaataa gttcaattt ttcgttcga 
      tcgctgttt taatgactg gaaactgaa caatatcat 
      gcggtattc gattttaaa accacattg tacacatag 
      atacatatg tcaaatagt aggttgtac gatctttaa 
      aaactacta aaaatttta cccttttat cgattaaga 
      ttttgacag gtttaatcg tggctgttc ttagttgtg 
      gacaaatag tagttccga cttccacta tcgtcaagt 
      ttgaactca acgtgaatt tttcttaat tttggacaa 
      tagtggaag ccccataat ggtgaaaat tcgatataa 
      tgacacatt tgtcttgta gttgtaaga tttaagtta 
      taaaatgac tggtaggac ttacattac gctctctcg 
      agtctcatt tgtatttat gaatagtta agtttattc 
      gatttttag tataagtgc aatggaaac taatttccc 
      acgtgttta gtgtatttt tagttagtt atattttga 
      tactctatt cttttttaa gcagatgtt gtaggtctg 
      catgacata tcgacgata atctctcta tattatagc 
      tctctagaa tgtacagtt ttttcaaag atctgtttt 
      taattgtgt aaaccctgg gatgtgttt tcgcatttg 
      ccatctgta aattttggt ttgatttat tcagttttg 
      ttgatttcc tgtgttgca acgcacaaa atgctttcc 
      gtgagtgtg aaaatacat ccttcaaat taaggccaa 
      taagctcta gtctctgta aatatttca tacgtacaa 
      aacccatcc tcttttggt tacgatccc aagaatttt 
      cggttttta tcagtatca tcccatttt attataata 
      acccccaat atattcata cctttttat gaataaaac 
      gtaaaagag gtacaagca caaaggctg cggtatttt 
      atttattga ccccgtcat cttaaaaaa cccgtgaat 
      aattgtgaa aaaatcgg
    • >TC033134 RB
      tctttagac ccaaaattt tgtcttcaa caacaccac 
      ctgattgta atttttttg gcacaaaaa ttcttatta 
      caaaactgt tctaaaatc taggccaga tccgagttt 
      ttaccataa acaaagtct attattggc ttattttaa 
      tatctaact ttttgtagt gggatttgg tcacaatct 
      gcatttgat aaattaatg tttcattat tacatacga 
      tagatttat tcttaaaac taaacttga gactaaaac 
      ttttttgaa ttagctttt gtattttgt tcgttgttt 
      atacaatat aatcgaatt taacttgtt tagtgtaaa 
      tgtagtagt cataataat tataaaaga aaacatgca 
      caagtgata aggaaatga agatttttt ggtttattg 
      gttgttgtt gggtttata ttaattagt ttaggtaat 
      aaaagagga agagaaatt atattcaga gtcaagtct 
      ttgtgagat ttattatta tgtaaattt tcaaataag 
      atttcaatg ttttggcaa gttttcaac taaaaatta 
      acttagttt attttgttt ttaaattga tttatgaaa 
      ctttcttga agttataaa acattccac aaaacagta 
      gtgattaag taagtgttg ttaagagat ttcatatta 
      ctggcccac agtagataa aatatttat attaattaa 
      tgaacaatc aaggcctgg taatgtgag atttttcca 
      tagaaatgc taaaacact ttaccatcc ttactgaat 
      ctaatatag tcaataatg caactgcaa gagtacaaa 
      ctaagaaac caccaacgc ttccaacag ttccggtaa 
      tacaatggt ccgatgcca cactgcgag ctggagtgg 
      cggccattc agagaggac gagcaaggg cagcatatc 
      cggcagccg ctgcagcag ctgcagccg catttgcga 
      ggcatccga cgcctttgg ctggagctt tacacggtt 
      acacccccg ttttgaaca cattacctg tagatggca 
      gcgtttact atgaccctt ttctggctg cccacgcag 
      ctgatccca attatagac tacaggctg ctgcggccg 
      caacacctc tattaaaaa cacctctgt cgactgccc 
      aacaggcga gttacgctg ccgctgcca cctatacgg 
      cagttgcgg cgcgttacg gagccgctc aacccgtcg 
      ccggttacg ccgctgtcg ccggttacg gccgggatt 
      acgctgatc cctacctgg gacacggga tcgggccga 
      tggccggat atggggctg ctgtgtatc ggagcggtt 
      ataaccggt tcacgccct attgaacat atccgcagc 
      tccgttagg cctacgaaa atactcaac tgttatttg 
      tcttgtgta cgtagcaca aaacagcac aaacaagaa 
      tgagccaaa gagctgtaa ctcactaac gtaagccct 
      tgtgatagc ccacctaac ccagctctc taacttagt 
      taaggatta gtcataagg tccgtttcc attcactta 
      tacggctac cttttactc gaaactgac tcaggtcgg 
      ttggaacgg taagtgagg atagttgta gggtattgc 
      aggacgtgt agtgttacg tactttatt ttaacttaa 
      gacatgtga cacaaatgc catgaccag gataattag 
      ctatcgttc agactgtta ttaagtacc ttactcagt 
      cacagaaag gcttgtgtt aggaatcat taggcgata 
      aagcaataa gttcaattt ttcgttcga tcgctgttt 
      taatgactg gaaactgaa caatatcat gcggtattc 
      gattttaaa accacattg tacacatag atacatatg 
      tcaaatagt aggttgtac gatctttaa aaactacta 
      aaaatttta cccttttat cgattaaga ttttgacag 
      gtttaatcg tggctgttc ttagttgtg gacaaatag 
      tagttccga cttccacta tcgtcaagt ttgaactca 
      acgtgaatt tttcttaat tttggacaa tagtggaag 
      ccccataat ggtgaaaat tcgatataa tgacacatt 
      tgtcttgta gttgtaaga tttaagtta taaaatgac 
      tggtaggac ttacattac gctctctcg agtctcatt 
      tgtatttat gaatagtta agtttattc gatttttag 
      tataagtgc aatggaaac taatttccc acgtgttta 
      gtgtatttt tagttagtt atattttga tactctatt 
      cttttttaa gcagatgtt gtaggtctg catgacata 
      tcgacgata atctctcta tattatagc tctctagaa 
      tgtacagtt ttttcaaag atctgtttt taattgtgt 
      aaaccctgg gatgtgttt tcgcatttg ccatctgta 
      aattttggt ttgatttat tcagttttg ttgatttcc 
      tgtgttgca acgcacaaa atgctttcc gtgagtgtg 
      aaaatacat ccttcaaat taaggccaa taagctcta 
      gtctctgta aatatttca tacgtacaa aacccatcc 
      tcttttggt tacgatccc aagaatttt cggttttta 
      tcagtatca tcccatttt attataata acccccaat 
      atattcata cctttttat gaataaaac gtaaaagag 
      gtacaagca caaaggctg cggtatttt atttattga 
      ccccgtcat cttaaaaaa cccgtgaat aattgtgaa 
      aaaatcgg

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY