Status
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Gene information
General information
TC008255 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7376074..7378498
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC008255
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08148

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head appendages random not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    antenna antennal flagellum not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    dorsal ridge row bristle dorsal present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008255 RA
      MSAHVVCVT SGGGLPIFS RKKGNTDSL PFSTIGSLN 
GVHMFGKSQ KITLQNTVT EDYSLAWKE YHDSLVLIG 
ISSGCTIEV LTKTLDSIF NAIVLIVGI EEIKSQRNI 
ERFKRELRI CYPLIDRLL DSLDCGDSG SRHSSDLIG 
LVETILCPE NHLIQIVLD SFTECVDSL FSCVLIHGK 
IAAATNSWW SLHPEEIKL LAILAQSEN TSSSKDIPV 
FLPYKSPTV AFRFVCCTL IPDIQLCCL CGPTPVLSE 
VEFSATNCF KNSADILSS AIQCHPRNF HSTCHIDGG 
ILGLLLVNT TYGKYMITR NPHQNSNKK NASSSHRLD 
ILRTFFYQS VLTFLVPSE DSNEDAEIS NKSEANVGL 
ETYWCSEYH KCFAMKIRD NILAVLFNS SIPTFAMRL 
ITEKTLKNL ISDKQICW
    CDS sequence
    • >TC008255 RA
      atgtcggca catgttgtt tgtgtcacc tcggggggt 
ggtctgccc atattttcc aggaagaaa gggaatacg 
gacagtctg cccttttca accattggg tccctaaat 
ggggtccac atgtttggc aaatcgcaa aaaatcacc 
cttcaaaat acggtaact gaagactac tctttagcc 
tggaaggag taccacgac agcttggtt ttgataggg 
atatcaagt ggatgcacc atcgaggtg ctcaccaag 
accctcgac tcgattttc aacgcaatc gtgttaatt 
gtcggaatc gaggaaatc aagtcgcag cgaaatatc 
gaacggttt aagagagaa ttgcggatt tgctaccct 
ttgatcgat cgcctgctg gattcttta gactgtggc 
gattctggc agcagacac tcgtcggat ttaatcgga 
ctggtggaa acgatttta tgtcccgaa aaccacttg 
attcagatc gttcttgac tcgtttacc gaatgcgtc 
gattcctta tttagctgc gtccttatt catggcaaa 
attgcggct gcgaccaac agctggtgg tcgctgcac 
ccagaagag atcaagctt ttggccatt ctggcccag 
agcgagaac acgtcgagt tcgaaagac atccccgtt 
ttcctgcca tacaaaagc ccaaccgtg gcttttcgt 
ttcgtttgc tgtacttta attccggac atccagctg 
tgttgtctc tgcggcccc actcccgtg ctctcggag 
gtggaattt tccgcgact aattgtttc aagaattcc 
gcagatatt ttaagttcc gccatacaa tgccacccg 
cgcaacttc cactcgact tgtcacatc gacggcggg 
attttgggt ttactgttg gtgaacacg acttacggc 
aaatacatg attacgaga aatccgcat cagaatagt 
aataaaaag aacgcgagc agctcgcat cgcttggat 
attttacga acgtttttc tatcaatcg gttttaacg 
ttccttgtg ccgagcgag gactcgaat gaagatgcg 
gaaatttcc aataaaagt gaagcgaat gtggggctt 
gaaacctac tggtgctcg gagtatcat aaatgcttc 
gcaatgaaa atcagggat aatattcta gcggtttta 
ttcaactcg tcgattccg acgttcgca atgcgactg 
attacggag aaaactctc aagaatttg atctcagat 
aaacaaatt tgttggtga
    mRNA sequence
    • >TC008255 RA
      aataaccag agatttgtc atggtggcc cacagaggg 
cgtcgactc gcttcaatc aaaacgctc cacagcgaa 
atttacggc caataatcg gccaatttc agtgtttta 
agcgccttt caggcccgc aatcgctgg gtcacccgg 
atttccggt gatgtatca atgtttact tgtacaata 
tttgcccta acccatgaa aaatgtcgg cacatgttg 
tttgtgtca cctcggggg gtggtctgc ccatatttt 
ccaggaaga aagggaata cggacagtc tgccctttt 
caaccattg ggtccctaa atggggtcc acatgtttg 
gcaaatcgc aaaaaatca cccttcaaa atacggtaa 
ctgaagact actctttag cctggaagg agtaccacg 
acagcttgg ttttgatag ggatatcaa gtggatgca 
ccatcgagg tgctcacca agaccctcg actcgattt 
tcaacgcaa tcgtgttaa ttgtcggaa tcgaggaaa 
tcaagtcgc agcgaaata tcgaacggt ttaagagag 
aattgcgga tttgctacc ctttgatcg atcgcctgc 
tggattctt tagactgtg gcgattctg gcagcagac 
actcgtcgg atttaatcg gactggtgg aaacgattt 
tatgtcccg aaaaccact tgattcaga tcgttcttg 
actcgttta ccgaatgcg tcgattcct tatttagct 
gcgtcctta ttcatggca aaattgcgg ctgcgacca 
acagctggt ggtcgctgc acccagaag agatcaagc 
ttttggcca ttctggccc agagcgaga acacgtcga 
gttcgaaag acatccccg ttttcctgc catacaaaa 
gcccaaccg tggcttttc gtttcgttt gctgtactt 
taattccgg acatccagc tgtgttgtc tctgcggcc 
ccactcccg tgctctcgg aggtggaat tttccgcga 
ctaattgtt tcaagaatt ccgcagata ttttaagtt 
ccgccatac aatgccacc cgcgcaact tccactcga 
cttgtcaca tcgacggcg ggattttgg gtttactgt 
tggtgaaca cgacttacg gcaaataca tgattacga 
gaaatccgc atcagaata gtaataaaa agaacgcga 
gcagctcgc atcgcttgg atattttac gaacgtttt 
tctatcaat cggttttaa cgttccttg tgccgagcg 
aggactcga atgaagatg cggaaattt ccaataaaa 
gtgaagcga atgtggggc ttgaaacct actggtgct 
cggagtatc ataaatgct tcgcaatga aaatcaggg 
ataatattc tagcggttt tattcaact cgtcgattc 
cgacgttcg caatgcgac tgattacgg agaaaactc 
tcaagaatt tgatctcag ataaacaaa tttgttggt 
gatggttta atatatttt ttgcaatat ctagtctct 
caattcctg gggtacggg tggcggcgt tgggggtgg 
tttattgtt cttaactct gccagtgta actcccgaa 
caaccaaac ggtgtaaga acgagctcg tccctgaaa 
cctacggct ctcaaaatg agccgtctg tattaacct 
gaacaaaat tagagtcac aacctgaaa aaaattata 
ataaaacaa gtttgattt tgacggagt cctacatac 
ctcaaggat aatatagag aattaaatt acccacaat 
aatccatcc aactgtttg ttttttttc gcggatcat 
ttcgtaaca tcctctcgt cgaaatgct caaattaaa 
ttcaggagt gtacaaata tgcttttcc tgtgacttc 
gatattgtg gtggataca gggagaaag tgatttcat 
agaacaaca actaaacgg tgtgttacc atcagcagc 
agaacttaa aaaccaatt tactaatta acgatgttt 
ttataattg tcgctttct acgctagat taggatac

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY