Status
Please wait ...
Gene information
General information
TC001032 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 9985830..9988585
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC001032
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00208

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection

    Fertility
    egg production: number no/almost no eggs -
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 2
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001032 RA
      MGERIEDYE VYNLLGKGG FASVYHAKC LKTGLNVAI 
KMIDKKMMQ AAGMVKRVE QEVSIHYRL KHPSILELY 
TFFEDANYV YLVLELCHN GELRQYIKT KALTESEVS 
SIMKQVVEG MKYLHTHNI LHRDISLSN LLLTKDMQV 
KIADFGLAT QLSRPDEKH TTMCGTPNF ISPEVLSRT 
SHGLEADVW GLGCLLYNL LVGSPPFDT HGVKNTLNR 
IVSANFHLP SHLSPEAKD LINSLLQKN PKDRIKLDQ 
ILEHPFITR GQLLMSHLT HDSGIHTMS SRRDSAFSE 
GASNNIYNF KSNSHNMEQ LSRKLHDVN LRSTYSDPP 
LFSNCGEIR SHCSCELPQ NNAYPADPG GNRILKDNS 
NYKPIFSTT SNSSSKSDK SKTQRLLQL CSQRLLPKT 
HRTENSILH IMEDGEVVV ELMKKKGSA KRNVVCDVL 
RISSDGAKI TIYEPNGGK GVTPGAAPP PIPPQGGYE 
AYTIENLPE KHWKKYMHA YQFVALIKE KTPKVTCYN 
DKMKCVLME NLTDFEATF YEGGTVTKS SLKGITFQD 
SSGNKLLVK TSNECRSLT GTFEYMWSQ SQEALKHCL 
LLEEMLSKL AGPNFPAII GRRPCSGPG KENQTQTTM 
MPSFAVSMN STAVGSFQS SSSLKEKKV SVPGVGTAV 
QLPNGEVQV RYGDGSQLS MDGKHQIKY QYSDGNTVT 
YSDNDNIPR PIREKLQHM PKILKQLMP SPTTHNFRL
    CDS sequence
    • >TC001032 RA
      atgggtgaa cgaattgag gactacgaa gtttacaat 
ttgttaggg aaaggaggt tttgcctcc gtatatcac 
gcaaaatgt ttaaaaacc ggattaaat gtcgctata 
aaaatgatc gacaagaaa atgatgcaa gcggctggg 
atggtaaaa cgtgtcgaa caggaagta tcaattcat 
tacagactt aaacaccct tctattttg gagttgtat 
acgtttttt gaggacgct aattatgtt tatctcgtt 
ttggagtta tgtcacaat ggggagctc aggcagtat 
atcaaaact aaggcatta actgagagt gaagtaagc 
agtattatg aaacaagtg gtagaaggc atgaaatat 
ctccacacc cacaatatt ctccacaga gacatttcc 
ttatcaaac ttattacta accaaagac atgcaagtc 
aaaattgca gattttggt ttggccaca caattatca 
cgtcctgat gaaaaacac acaacaatg tgtggaacc 
ccaaatttt atctcaccc gaagttctt tcacggact 
tcccacggc cttgaagcc gacgtttgg ggccttggt 
tgtctcctc tataattta ttagtgggc tcaccacct 
tttgacact cacggtgtt aaaaatact ctaaatcgc 
atcgtttcg gccaatttt catttaccc agtcatttg 
tcaccagaa gctaaggac ttaattaat tcacttcta 
cagaaaaat cctaaagat cgcatcaag ttggaccag 
attttggag caccctttt ataacaaga ggacagttg 
ttgatgtca catttgact catgatagt gggattcac 
acaatgtct agccgacgt gatagcgct tttagcgaa 
ggtgctagt aataatatt tacaatttt aaaagcaac 
tctcataat atggaacaa ctgtcgcgg aaacttcac 
gatgttaat ttgaggtcg acctattcg gaccctcca 
ctgttctca aattgtggc gaaattcgg agccactgc 
agttgcgaa ctgcctcaa aataacgca tatcccgct 
gatcctgga gggaacagg atcctcaaa gacaactcg 
aactacaaa ccaattttt tctacaact agtaacagt 
tcgagtaaa agtgacaag agtaaaacc cagcgattg 
ttgcaactg tgttcgcaa agactactt ccgaaaacg 
caccgaacc gaaaattcc attttacac ataatggaa 
gacggtgaa gtcgtcgtg gaactgatg aagaaaaaa 
ggaagtgcg aaacgtaac gtcgtttgt gatgtttta 
cgcatatct tcagacggg gccaaaatc actatttac 
gaacctaac ggcggtaaa ggggtcacc ccgggggcg 
gctccgccc ccaataccc ccacaaggg ggctacgaa 
gcatacact atcgaaaat ttaccggaa aaacactgg 
aagaagtac atgcacgcg taccaattc gtggccctt 
attaaggaa aaaacccca aaggttacg tgttacaat 
gataagatg aagtgcgtt ctgatggag aatttgacc 
gattttgag gccactttt tacgaaggt ggaaccgtg 
acgaaatcg tctttgaaa gggattacg tttcaagac 
tcgtccggt aacaaactt ttggtcaaa acaagtaac 
gaatgccgg agtttgacc ggaactttt gagtatatg 
tggtcgcag tcgcaggag gctttgaaa cactgcctc 
cttttggag gagatgttg tcaaagttg gctgggccc 
aattttccc gctattata ggcaggagg ccgtgctcg 
gggccaggg aaggagaat cagacccag actaccatg 
atgccctcg tttgctgtt tctatgaac agtacggct 
gtagggtcg tttcagagc agttcctct ttgaaggag 
aagaaggtg tcagtgcca ggggtgggc acggccgtg 
caattgccc aatggagaa gtgcaagta aggtatggt 
gacggatcc cagttgtca atggacggt aaacaccaa 
atcaaatat cagtattcg gacgggaat accgttacg 
tatagtgat aatgataat atcccgcgt ccgataagg 
gaaaagttg caacacatg ccaaaaatt ttgaaacag 
ttgatgccc agtccgact actcataat tttcggtta 
tag
    mRNA sequence
    • >TC001032 RA
      gtttttgaa aaacatttg ttttgtttt cagttattt 
tcaaatgat tttcggctg aaatcaatc aattttgac 
ccaaaaatg cattaacaa agtaaaaat tccttcctg 
agcccattg agaggtgat tttaaatgt ttctcgacg 
tggtttcac cttcaaact caaatgaat ctcgaaatg 
ggtgaacga attgaggac tacgaagtt tacaatttg 
ttagggaaa ggaggtttt gcctccgta tatcacgca 
aaatgttta aaaaccgga ttaaatgtc gctataaaa 
atgatcgac aagaaaatg atgcaagcg gctgggatg 
gtaaaacgt gtcgaacag gaagtatca attcattac 
agacttaaa cacccttct attttggag ttgtatacg 
ttttttgag gacgctaat tatgtttat ctcgttttg 
gagttatgt cacaatggg gagctcagg cagtatatc 
aaaactaag gcattaact gagagtgaa gtaagcagt 
attatgaaa caagtggta gaaggcatg aaatatctc 
cacacccac aatattctc cacagagac atttcctta 
tcaaactta ttactaacc aaagacatg caagtcaaa 
attgcagat tttggtttg gccacacaa ttatcacgt 
cctgatgaa aaacacaca acaatgtgt ggaacccca 
aattttatc tcacccgaa gttctttca cggacttcc 
cacggcctt gaagccgac gtttggggc cttggttgt 
ctcctctat aatttatta gtgggctca ccacctttt 
gacactcac ggtgttaaa aatactcta aatcgcatc 
gtttcggcc aattttcat ttacccagt catttgtca 
ccagaagct aaggactta attaattca cttctacag 
aaaaatcct aaagatcgc atcaagttg gaccagatt 
ttggagcac ccttttata acaagagga cagttgttg 
atgtcacat ttgactcat gatagtggg attcacaca 
atgtctagc cgacgtgat agcgctttt agcgaaggt 
gctagtaat aatatttac aattttaaa agcaactct 
cataatatg gaacaactg tcgcggaaa cttcacgat 
gttaatttg aggtcgacc tattcggac cctccactg 
ttctcaaat tgtggcgaa attcggagc cactgcagt 
tgcgaactg cctcaaaat aacgcatat cccgctgat 
cctggaggg aacaggatc ctcaaagac aactcgaac 
tacaaacca attttttct acaactagt aacagttcg 
agtaaaagt gacaagagt aaaacccag cgattgttg 
caactgtgt tcgcaaaga ctacttccg aaaacgcac 
cgaaccgaa aattccatt ttacacata atggaagac 
ggtgaagtc gtcgtggaa ctgatgaag aaaaaagga 
agtgcgaaa cgtaacgtc gtttgtgat gttttacgc 
atatcttca gacggggcc aaaatcact atttacgaa 
cctaacggc ggtaaaggg gtcaccccg ggggcggct 
ccgccccca ataccccca caagggggc tacgaagca 
tacactatc gaaaattta ccggaaaaa cactggaag 
aagtacatg cacgcgtac caattcgtg gcccttatt 
aaggaaaaa accccaaag gttacgtgt tacaatgat 
aagatgaag tgcgttctg atggagaat ttgaccgat 
tttgaggcc actttttac gaaggtgga accgtgacg 
aaatcgtct ttgaaaggg attacgttt caagactcg 
tccggtaac aaacttttg gtcaaaaca agtaacgaa 
tgccggagt ttgaccgga acttttgag tatatgtgg 
tcgcagtcg caggaggct ttgaaacac tgcctcctt 
ttggaggag atgttgtca aagttggct gggcccaat 
tttcccgct attataggc aggaggccg tgctcgggg 
ccagggaag gagaatcag acccagact accatgatg 
ccctcgttt gctgtttct atgaacagt acggctgta 
gggtcgttt cagagcagt tcctctttg aaggagaag 
aaggtgtca gtgccaggg gtgggcacg gccgtgcaa 
ttgcccaat ggagaagtg caagtaagg tatggtgac 
ggatcccag ttgtcaatg gacggtaaa caccaaatc 
aaatatcag tattcggac gggaatacc gttacgtat 
agtgataat gataatatc ccgcgtccg ataagggaa 
aagttgcaa cacatgcca aaaattttg aaacagttg 
atgcccagt ccgactact cataatttt cggttatag 
ttttactat tgttcattg aaaatgaaa tgcgttttg 
taacgagct gaccagttt ttcattgca tagagtaag 
atttttaaa aattgaata aatattttt gcactatta 
attgatgac actgttttt atttgtgac aataaagat 
tgaacttta tg

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY