adult nutritional state bad (cachectic)

adult lethality


mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number no/almost no eggs

adult lethality

eclosion not fulfilled (with pupal and adult features)


head & thorax & abdomen segment random not present

head appendages random not present

head appendages shape irregular

head & thorax & abdomen segment shape irregular

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature random pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


head capsule shape irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


larva number hatched many (ca. 70-100 %)


adult lethality


affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


antenna pedicellus not present

thorax & abdomen dorsal not fused

thorax & abdomen ventral not fused

legs pretarsus not present

antenna antennal flagellum not present

larva segment size decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


thorax & abdomen segment thinner

cuticle remnants with unidentifyable segments

thorax & abdomen segment number decreased

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

adult lethality immediately after eclosion


larva everted (inside-out)

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


thorax segment number decreased

abdomen segment number decreased

metathorax potentially constricted

head appendages partially not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

adult lethality


adult lethality

adult lethality

adult lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


abdominal odoriferous glands content split

abdominal odoriferous glands size decreased

adult lethality


adult lethality

adult lethality

adult lethality

adult lethality


abdomen partially everted (inside-out)

abdomen posterior circumference decreased

legs bent

antenna bent

affected eggs/embryos/larvae

abdominal odoriferous glands colour lighter

adult lethality

larval musculature random rounded

larval musculature random not present

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

adult lethality

vitellarium size decreased

egg chamber number decreased

mature egg not present

adult lethality

egg production: number no/almost no eggs

adult lethality


affected eggs/embryos/larvae

adult lethality

mature egg not present

yolk late structure irregular

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

eclosion not fulfilled (with pupal and adult features)


vitellogenic egg chamber late potentially degenerated

vitellogenic egg chamber late number increased

mature egg not present

adult lethality

egg chamber number decreased


adult lethality

larval musculature thinner

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected


adult lethality


vitellarium size decreased

yolk position anteriorized

vitellogenic egg chamber potentially degenerated

vitellogenic egg chamber number decreased

previtellogenic egg chamber not present

tropharium structure irregular

mature egg not present

larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

egg chamber mostly not present

vitellarium size decreased

mature egg not present

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

yolk late potentially degenerated

mature egg not present

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


abdomen partially dorsal not fused

head capsule size decreased

abdomen segment number decreased

thorax segment number decreased

head appendages partially not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


vertex triplet vertex triplet posterior sometimes potentially duplicated

affected eggs/embryos/larvae

larval musculature pattern irregular

larval musculature fluorescence decreased

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


legs podomer number decreased

thorax & abdomen dorsal not fused

legs size decreased

head appendages shape irregular

larva dorsal bent

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


legs tibiotarsus sometimes potentially fused

legs femur sometimes potentially fused

legs sometimes size decreased

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


legs potentially size decreased

larva cuticle thinner

Cuticle is too faint to analyze it properly

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

ovary without detectable defects

mature egg not present

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


thorax not present

head appendages number decreased

head capsule size decreased

head not present

abdomen segment number decreased

thorax segment number decreased

procephalic appendages not present

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

Nervous system outside of larvae

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult lethality

adult lethality


cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected


previtellogenic egg chamber not present

yolk fragmented

egg chamber potentially degenerated

egg chamber amount decreased

mature egg not present


vitellogenic egg chamber sometimes fused

follicular epithelium central potentially amount decreased

follicular epithelium bilateral potentially amount increased

yolk late potentially decreased

previtellogenic egg chamber not present

mature egg not present

egg production: number no/almost no eggs

eclosion not fulfilled (with pupal and adult features)


adult lethality

adult lethality


adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality


affected eggs/embryos/larvae

Nearly all of them look like elongated germ band stage

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality


ovary without detectable defects

adult lethality

egg production: number <50% of expected

adult lethality


abdomen segment number decreased

affected eggs/embryos/larvae

ovary without detectable defects

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


yolk sometimes amount decreased

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


previtellogenic egg chamber late not present

previtellogenic egg chamber early amount increased

previtellogenic egg chamber position irregular

vitellogenic egg chamber not present

tropharium structure irregular

mature egg not present

adult lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

vitellogenic egg chamber potentially degenerated

vitellogenic egg chamber sometimes number decreased

mature egg not present

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality


adult lethality

ovary without detectable defects

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:


larva number hatched many (ca. 70-100 %)


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


abdomen potentially size decreased

abdomen shape irregular

affected eggs/embryos/larvae

adult lethality


head capsule posterior shape thinner

posterior terminus potentially shape irregular

labrum posterior shape rounded

larva potentially shape irregular

affected eggs/embryos/larvae

larval musculature not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

ovary without detectable defects

previtellogenic egg chamber not present

mature egg not present

egg production: number <50% of expected


adult lethality

adult lethality

adult lethality


larval musculature potentially thinner

larval musculature fluorescence decreased

affected eggs/embryos/larvae


head & thorax appendages shape irregular

legs tibiotarsus size decreased

head capsule size decreased

head & thorax & abdomen segment random not present

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


abdominal odoriferous glands content darker

No offspring.

head appendages partially random not present

head appendages random shape irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

ovary without detectable defects

larva number few (< 50 %) hatched

egg production: number <50% of expected


adult lethality

larva number few (< 50 %) hatched


adult lethality

adult lethality

egg production: number no/almost no eggs

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality


head & thorax & abdomen appendages random not present

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae


adult lethality

adult nutritional state bad (cachectic)


bell row number decreased

vertex triplet vertex triplet posterior complete not present

larva dorsal bent

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


yolk potentially decreased

mature egg not present

previtellogenic egg chamber potentially not present

adult lethality


mature egg not present

previtellogenic egg chamber shape irregular

adult lethality


ovary without detectable defects

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult nutritional state bad (cachectic)

larva number hatched many (ca. 70-100 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


larva random shape irregular

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


head & thorax & abdomen appendages random partially not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larval musculature fluorescence not present

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)

adult lethality


previtellogenic egg chamber number decreased

adult lethality


egg chamber circumference decreased

ovariole shape irregular

vitellogenic egg chamber degenerated

adult lethality

adult lethality

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


egg chamber circumference decreased

ovariole shape irregular

vitellogenic egg chamber degenerated

egg production: number no/almost no eggs

adult lethality

eclosion not fulfilled (with pupal and adult features)


affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

mature egg not present

vitellogenic egg chamber degenerated

adult nutritional state bad (cachectic)

adult lethality


adult lethality


vertex triplet vertex triplet anterior complete not present

antenna antennal flagellum not present

antenna shape irregular

antenna shape bent

labrum complete not present

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

larva number hatched none (< 10 %)


adult lethality

adult lethality

adult lethality


larva partially everted (inside-out)

larva complete everted (inside-out)

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


abdominal odoriferous glands mostly content decreased

prothoracic odoriferous glands colour darker

abdominal odoriferous glands colour darker

mature egg not present

ovary without detectable defects

adult nutritional state bad (cachectic)


previtellogenic egg chamber complete not present

ovary without detectable defects

adult lethality


head & thorax & abdomen segment random not present

affected eggs/embryos/larvae

larval musculature random pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

adult lethality


larva number hatched many (ca. 70-100 %)

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected


cuticle remnants with some identifiable segments

affected eggs/embryos/larvae


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


abdomen tracheal opening mostly not present

abdomen segment shape irregular

legs segment potentially fused

legs pretarsus present

larva potentially everted (inside-out)

larva bristle mostly not present

larva appendages shape irregular

abdomen segment sometimes number decreased

legs segment number decreased

head appendages shape swollen

larva segment number decreased

cuticle remnants strongly fragmented (cuticle crumbs)

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

yolk ectopically early present

yolk deposition irregular

vitellogenic egg chamber potentially structure degenerated

vitellogenic egg chamber late number decreased

mature egg not present

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality

adult lethality


labrum size decreased

abdomen segment number decreased

legs podomer sometimes fused

head appendages orientation irregular

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality


abdominal odoriferous glands content opaque

adult lethality

tropharium anterior potentially structure irregular

vitellogenic egg chamber early without detectable defects

mature egg not present

vitellogenic egg chamber late not present

larva number hatched none (< 10 %)


labrum potentially not present

labium not present

mandible not present

maxilla not present

legs shortened

head capsule mostly not present

head & thorax cuticle structure irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality


adult lethality


cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality


affected eggs/embryos/larvae

adult lethality


adult lethality

adult lethality

adult lethality


affected eggs/embryos/larvae

vitellogenic egg chamber late mostly not present

mature egg not present

vitellogenic egg chamber early number increased

larva number hatched none (< 10 %)


adult lethality

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


previtellogenic egg chamber number increased

previtellogenic egg chamber orientation irregular

mature egg not present

egg chamber number decreased

vitellarium size decreased

tropharium structure irregular

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva number hatched

adult lethality

egg production: number no/almost no eggs


abdominal odoriferous glands content irregular

adult lethality

adult lethality


adult lethality

yolk potentially late deposition irregular

mature egg number increased

mature egg structure irregular

ovary without detectable defects

egg production: number <50% of expected

adult lethality


larva number few (< 50 %) hatched

adult lethality


adult lethality


affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


cuticle remnants strongly fragmented (cuticle crumbs)

affected eggs/embryos/larvae

affected eggs/embryos/larvae

adult nutritional state potentially bad (cachectic)

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


previtellogenic egg chamber potentially not present

vitellogenic egg chamber potentially position anteriorized

vitellarium shortened

vitellogenic egg chamber late degenerated

mature egg not present

larva number hatched none (< 10 %)


larva number hatched many (ca. 70-100 %)


legs size decreased

head capsule size decreased

affected eggs/embryos/larvae

adult lethality

adult lethality

legs femur potentially fused

legs tibiotarsus potentially fused

antenna antennal flagellum not present

antenna shape irregular

urogomphi orientation irregular


larval musculature thinner

larval musculature fluorescence decreased

larval musculature pattern irregular

larval musculature mostly not present

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


larval musculature thinner

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


ovary without detectable defects

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality

adult lethality

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (died as pupa)


larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


ovary without detectable defects

adult lethality


affected eggs/embryos/larvae

odoriferous glands colour darker

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality


adult lethality

egg production: number <50% of expected

adult lethality


affected eggs/embryos/larvae

adult lethality


legs unilateral not present

head mostly not present

thorax not present

labium structure irregular

head appendages shape irregular

legs tibiotarsus potentially fused

legs femur potentially fused

legs shortened

abdomen shape irregular

thorax segment number decreased

head capsule size decreased

head & thorax unilateral fused

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

larval musculature partially rounded

larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality


larval musculature thinner

best to be seen on the dorsal side

affected eggs/embryos/larvae

ovary without detectable defects

egg production: number <50% of expected

adult lethality

adult lethality


larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality


abdominal odoriferous glands partially colour darker

abdominal odoriferous glands slightly content fragmented

adult lethality

adult lethality

adult lethality

eclosion not fulfilled (with pupal and adult features)


mandible orientation irregular

legs cuticle structure irregular

head appendages shape irregular

affected eggs/embryos/larvae

larval musculature random not present

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

eclosion not fulfilled (died as pupa)


abdomen tracheal opening size decreased

mesothoracic tracheal opening not present

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality


head capsule size decreased

head & thorax potentially partially fused

head appendages random not present

legs number decreased

thorax anterior segmentation/division irregular

abdomen bent

antenna not present

head capsule mostly not present

abdomen broader

thorax anterior partially not present

head appendages orientation irregular

head & thorax segmentation/division irregular

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality


legs swollen

legs femur potentially fused

legs tibiotarsus potentially fused

posterior terminus structure irregular

abdomen shape irregular

mandible not present

thorax segment partially not present

abdomen segment number decreased

thorax segment not present

head capsule mostly not present

larva segment number decreased

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

adult lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


adult lethality

previtellogenic egg chamber potentially number decreased

egg chamber structure degenerated

tropharium structure irregular

tropharium structure irregular

vitellogenic egg chamber colour darker

mature egg not present

larva number hatched none (< 10 %)


adult lethality

mature egg not present

vitellogenic egg chamber late sometimes number increased

vitellogenic egg chamber late sometimes shape irregular

yolk late amount decreased

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality immediately after eclosion


affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

adult nutritional state potentially bad (cachectic)

larva number few (< 50 %) hatched

adult lethality


adult lethality

tropharium structure irregular

egg chamber position anteriorized

tropharium shape broader

mature egg not present

egg chamber position irregular

egg chamber potentially number decreased

vitellarium size decreased

ovary size decreased

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality


larval musculature thinner

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

adult lethality


affected eggs/embryos/larvae

adult lethality

affected eggs/embryos/larvae

tropharium structure irregular

yolk structure irregular

mature egg not present

adult lethality


affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

yolk deposition irregular

vitellogenic egg chamber sometimes structure irregular

vitellogenic egg chamber late number increased

mature egg not present

egg production: number no/almost no eggs

adult lethality


larva bristle pattern irregular

larva segment number decreased

legs fused

legs structure irregular

legs sometimes number decreased

thorax segment unilateral potentially fused

abdomen segment number decreased

head capsule size decreased

head & thorax partially potentially fused

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

larval musculature fluorescence decreased

affected eggs/embryos/larvae

larva number hatched many (ca. 70-100 %)

adult lethality


head appendages potentially random not present

head appendages shape irregular

cuticle remnants strongly fragmented (cuticle crumbs)

head capsule not present

cuticle remnants with unidentifyable segments

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

adult lethality

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

adult lethality


larva number few (< 50 %) hatched

adult lethality


embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched

adult lethality


adult lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

adult nutritional state

adult nutritional state bad (cachectic)


adult lethality

adult lethality

adult lethality

adult lethality


affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


abdominal odoriferous glands content decreased

abdominal odoriferous glands anterior partially content darker

affected eggs/embryos/larvae

odoriferous glands colour darker

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

egg production: number <50% of expected

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


eclosion not fulfilled (with pupal and adult features)


vertex triplet orientation irregular

antenna position irregular

antenna shape irregular

labrum partially not present

head capsule shape irregular

affected eggs/embryos/larvae


head appendages partially random not present

head capsule not present

labrum not present

head & thorax & abdomen segment partially random not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


abdominal odoriferous glands partially content not present

abdominal odoriferous glands content not present

abdominal odoriferous glands content darker

adult lethality


abdominal odoriferous glands structure irregular

abdominal odoriferous glands partially content darker

prothoracic odoriferous glands colour darker

abdominal odoriferous glands colour darker

larva number hatched many (ca. 70-100 %)


adult lethality

adult lethality


abdominal odoriferous glands partially not present

abdominal odoriferous glands mostly colour lighter


head appendages shape irregular

legs size shortened

legs shape irregular

antenna antennal flagellum not present

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality


abdominal odoriferous glands shape irregular

abdominal odoriferous glands content decreased

abdominal odoriferous glands anterior partially content darker

odoriferous glands colour darker

eclosion not fulfilled (with pupal and adult features)


head & thorax & abdomen appendages random not present

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality


thorax constricted

head & thorax & abdomen segment random not present

affected eggs/embryos/larvae


thorax musculature not present

head musculature not present

abdomen number decreased

thorax constricted

head capsule split

head & thorax segment random not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

egg production: number <50% of expected


vitellarium structure split

lateral oviduct partially deposition degenerated

vitellarium potentially degenerated

egg production: number no/almost no eggs

adult lethality


cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched

adult lethality


abdominal odoriferous glands anterior content darker

prothoracic odoriferous glands colour darker

abdominal odoriferous glands colour darker

larva number hatched many (ca. 70-100 %)

adult lethality


larva everted (inside-out)

head appendages random not present

adult lethality

head capsule complete not present

head & thorax appendages shape irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


abdominal odoriferous glands potentially not present

abdominal odoriferous glands content not present

abdominal odoriferous glands content decreased

abdominal odoriferous glands partially colour darker

adult lethality


vitellogenic egg chamber late deposition not present

vitellarium size decreased

previtellogenic egg chamber number decreased

eclosion not fulfilled (with pupal and adult features)


vitellarium size decreased


head & thorax & abdomen segment random not present

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature random not present

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


larva dorsal not closed

head appendages shape irregular

head & thorax & abdomen segment partially not present

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

adult lethality

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality


adult lethality

ovary without detectable defects

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

adult nutritional state

egg production: number no/almost no eggs


adult lethality

adult lethality


larva segment random not present

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality


adult lethality

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

vitellogenic egg chamber stage sequence irregular

previtellogenic egg chamber number increased

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

vitellarium anterior swollen

adult lethality

larva number hatched none (< 10 %)


adult lethality

adult lethality

adult lethality


posterior terminus not present

abdomen segment transformed

legs size decreased

legs shape swollen

head appendages partially not present

Head appendages are mostly not present except antenna & mandible. Antenna & mandible segments are partially not present.

head capsule size decreased

mandible shape swollen

abdomen & terminus segment partially not present

Head segments are also partially absent. It's not possible to use head, abdomen & terminus combination in the annotation.

head & thorax appendages size shortened

abdomen segment number decreased

legs number increased

antenna shape swollen

head & thorax appendages shape swollen

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

mature egg potentially not present

previtellogenic egg chamber number decreased

previtellogenic egg chamber anterior partially shape constricted

adult lethality

eclosion not fulfilled (with pupal and adult features)


abdominal odoriferous glands content not present

abdominal odoriferous glands content decreased

abdominal odoriferous glands necrotic

abdominal odoriferous glands anterior content darker

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

odoriferous glands colour darker

larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

adult nutritional state bad (cachectic)

egg production: number no/almost no eggs

adult lethality


ovary without detectable defects

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched many (ca. 70-100 %)


vitellogenic egg chamber potentially degenerated

vitellogenic egg chamber number decreased

vitellogenic egg chamber potentially fused

previtellogenic egg chamber not present

vitellarium size decreased

mature egg not present

adult lethality


yolk late potentially degenerated

mature egg not present

adult lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


posterior terminus potentially transformed

posterior terminus not present

abdomen segment number increased

affected eggs/embryos/larvae

prothorax musculature specific subset not present

affected eggs/embryos/larvae

potentially higher penetrance, but not all larvae are observable from the ventral side

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality


egg chamber not present

vitellarium size decreased

mature egg not present

adult lethality

adult lethality


abdominal odoriferous glands size decreased

abdomen cuticle dorsal partially not present

thorax & abdomen cuticle posterior segment boundary irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

eclosion not fulfilled (with pupal and adult features)


previtellogenic egg chamber amount decreased

vitellogenic egg chamber sometimes potentially fused

vitellarium anterior circumference decreased

mature egg not present

adult lethality


adult lethality


adult nutritional state bad (cachectic)

adult lethality

adult lethality


adult lethality

adult nutritional state bad (cachectic)

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


ovary without detectable defects

mature egg deposition 4 >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

egg production: number <50% of expected

adult lethality


maxillary palp size increased

antenna scapus proximal slightly circumference decreased

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

adult lethality immediately after eclosion


larva number hatched many (ca. 70-100 %)

adult lethality


mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality


previtellogenic egg chamber sometimes not present

vitellogenic egg chamber sometimes potentially degenerated

vitellogenic egg chamber amount decreased

vitellogenic egg chamber late sometimes amount increased

vitellogenic egg chamber potentially sometimes fused

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

adult lethality

adult lethality


adult nutritional state bad (cachectic)

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

cuticle remnants with some identifiable segments


head appendages number decreased

cuticle remnants with unidentifyable segments

thorax segment number decreased

head capsule size decreased

affected eggs/embryos/larvae

larval musculature fluorescence decreased

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


egg production: number <50% of expected

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

ovary without detectable defects

yolk late sometimes amount decreased

mature egg not present

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


previtellogenic egg chamber mostly number decreased

vitellogenic egg chamber potentially degenerated

mature egg not present

egg production: number no/almost no eggs

adult lethality

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality


cuticle remnants strongly fragmented (cuticle crumbs)

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

larva number hatched many (ca. 70-100 %)


adult lethality

adult nutritional state potentially bad (cachectic)

egg production: number <50% of expected

adult lethality


larva partially everted (inside-out)

larva everted (inside-out)

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

egg production: number <50% of expected


adult lethality

adult lethality


legs size decreased

head appendages shape irregular

abdomen potentially circumference decreased

abdomen potentially everted (inside-out)

thorax potentially partially everted (inside-out)

antenna potentially everted (inside-out)

thorax & abdomen potentially partially everted (inside-out)

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 4 5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (with pupal and adult features)


cuticle remnants with unidentifyable segments

abdomen segment number decreased

thorax segment number decreased

head appendages partially not present

thorax & abdomen segment number decreased

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched


abdomen segment number decreased

thorax segment number decreased

head capsule size decreased

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality

adult lethality

adult lethality

eclosion not fulfilled (died as pupa)


affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


legs sometimes partially size decreased

head appendages number decreased

head capsule size decreased

prothorax not present

head not present

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


egg chamber number decreased

mature egg not present

adult lethality


adult lethality

tropharium structure irregular

egg chamber number decreased

adult nutritional state bad (cachectic)


affected eggs/embryos/larvae

adult lethality

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

larval musculature dorsal lateral thinner

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

adult lethality

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


vertex triplet vertex triplet anterior not present

labrum not present

affected eggs/embryos/larvae

larva number hatched many (ca. 70-100 %)


adult lethality


affected eggs/embryos/larvae

larva number hatched none (< 10 %)


head & thorax & abdomen segment shape irregular

head & thorax & abdomen segment random not present

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


antenna potentially shape swollen

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)


adult lethality

egg production: number no/almost no eggs

adult lethality

adult lethality


adult lethality

adult nutritional state bad (cachectic)

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality


egg chamber potentially degenerated

ovary without detectable defects

egg chamber mostly not present

mature egg not present


adult lethality

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

adult lethality

adult lethality


adult lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

abdominal odoriferous glands less secretion


egg chamber potentially fused

follicular epithelium potentially not present

ovary without detectable defects

eclosion not fulfilled (with pupal and adult features)


abdomen constricted

Constriction often separates head and thorax from abdomen segments.

head capsule not present

affected eggs/embryos/larvae

larval musculature fluorescence decreased

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


egg chamber mostly not present

mature egg not present

adult lethality

egg production: number <50% of expected

adult lethality


head & thorax segment not present

head capsule shape irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


abdomen segment constricted

head appendages position irregular

affected eggs/embryos/larvae

adult lethality


adult lethality

adult lethality


head capsule not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality


head appendages position irregular

legs potentially shape irregular

head capsule not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


procephalic appendages mostly not present

head appendages position irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


head appendages position irregular

affected eggs/embryos/larvae

adult lethality


cuticle remnants strongly fragmented (cuticle crumbs)

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


head capsule not present

affected eggs/embryos/larvae

adult lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected


affected eggs/embryos/larvae

larval musculature fluorescence not present

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

mature egg not present

egg chamber not present

adult nutritional state potentially bad (cachectic)

adult lethality


legs shape irregular

legs potentially constricted

head capsule not present

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality


larva number hatched many (ca. 70-100 %)

adult lethality


affected eggs/embryos/larvae


head appendages random not present

head & thorax segment not present

head & thorax & abdomen segment random not present

head not present

head & thorax & abdomen segment shape irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

adult lethality

adult nutritional state

egg production: number no/almost no eggs

adult lethality

adult lethality


adult lethality

adult lethality

adult lethality


mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality


adult lethality


previtellogenic egg chamber potentially not present

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

eclosion not fulfilled (with pupal and adult features)


affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae


adult lethality

mature egg not present

egg chamber not present

adult lethality

adult nutritional state

adult lethality


adult lethality

adult lethality

adult nutritional state bad (cachectic)

adult lethality


head & thorax segment potentially random not present

abdomen segment number decreased

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


tropharium potentially bent

tropharium potentially size decreased


abdomen segment number decreased

head appendages potentially not present

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


head appendages position irregular

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult lethality


cuticle remnants with some identifiable segments

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

mature egg not present

vitellogenic egg chamber late potentially degenerated

vitellogenic egg chamber late sometimes structure irregular

vitellogenic egg chamber sometimes late number increased

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

adult lethality immediately after eclosion


adult lethality

larva number hatched many (ca. 70-100 %)

adult lethality


cuticle remnants with unidentifyable segments

cuticle remnants strongly fragmented (cuticle crumbs)

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality


affected eggs/embryos/larvae

adult lethality

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 5 >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

adult lethality


previtellogenic egg chamber late sometimes shape irregular

yolk late deposition irregular

vitellogenic egg chamber late number increased

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


adult lethality


labrum mostly not present

urogomphi position anteriorized

labium not present

antenna not present

posterior terminus shape irregular

prothorax unilateral not present

head capsule size decreased

larva bristle random number increased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

adult lethality

adult lethality


larval musculature potentially thinner

larval musculature fluorescence decreased

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

egg production: number <50% of expected

adult lethality


head capsule potentially partially not present

head appendages not present

head appendages random structure irregular

gena triplet bristle potentially number increased

legs podomer swollen

head capsule size decreased

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

adult lethality


abdomen segment number decreased

thorax segment number decreased

head shape irregular

thorax & abdomen segment number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


vitellarium potentially size decreased

egg chamber potentially number decreased

egg chamber stage sequence irregular

vitellogenic egg chamber late not present

mature egg not present

tropharium structure irregular

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

adult lethality

adult lethality immediately after eclosion


larva number hatched many (ca. 70-100 %)


larval musculature potentially slightly thinner

affected eggs/embryos/larvae

adult lethality


labium not present

mandible not present

larva potentially everted (inside-out)

cuticle remnants with unidentifyable segments

head capsule mostly not present

larva dorsal bent

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality


abdominal odoriferous glands content fragmented

odoriferous glands slightly colour darker

adult lethality

eclosion not fulfilled (with pupal and adult features)

eclosion not fulfilled (died as pupa)

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


vitellogenic egg chamber sometimes structure degenerated

yolk late amount decreased

larva number hatched none (< 10 %)

egg production: number no/almost no eggs


larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

previtellogenic egg chamber orientation irregular

mature egg not present

previtellogenic egg chamber number increased

tropharium structure irregular

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality


legs size decreased

legs number decreased

thorax potentially transformed

abdomen segment number increased

thorax segment number decreased

head appendages segmentation/division present

head & thorax potentially transformed

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

abdomen potentially multiplied

head potentially transformed

head not present

legs not present


adult lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

adult lethality

adult lethality


adult lethality

adult nutritional state bad (cachectic)


affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


legs tibiotarsus potentially partially fused

legs femur potentially partially fused

legs size decreased

antenna antennal flagellum not present

affected eggs/embryos/larvae

larval musculature lateral thinner

larval musculature pattern irregular

larval musculature fluorescence decreased

affected eggs/embryos/larvae

mature egg not present

yolk late mostly amount decreased

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


cuticle remnants strongly fragmented (cuticle crumbs)

larva potentially everted (inside-out)

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

tropharium structure irregular

egg chamber mostly not present

mature egg not present

adult lethality


larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

adult lethality


adult nutritional state bad (cachectic)

adult lethality

adult lethality


adult lethality

adult lethality

adult lethality

adult lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

yolk mostly amount decreased

egg chamber stage sequence irregular

previtellogenic egg chamber number decreased

mature egg not present

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality


adult lethality

egg production: number <50% of expected

adult lethality


adult lethality

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult nutritional state potentially bad (cachectic)

adult nutritional state bad (cachectic)

adult lethality

adult lethality


abdomen segment sometimes constricted

abdomen segment number decreased

affected eggs/embryos/larvae

larval musculature partially swollen

larval musculature thinner

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

adult lethality


adult lethality

adult lethality

adult lethality


adult lethality


head bristle number increased

legs partially bent

abdomen segment number decreased

thorax segment number decreased

head appendages partially bent

thorax & abdomen not present

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

adult lethality


eclosion not fulfilled (with pupal and adult features)


adult lethality

adult lethality

eclosion not fulfilled (died as pupa)


vitellogenic egg chamber mostly not present

mature egg not present

egg production: number no/almost no eggs

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)


egg chamber mostly not present

mature egg not present

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


follicular epithelium potentially amount decreased

vitellarium size decreased

vitellogenic egg chamber potentially degenerated

vitellogenic egg chamber amount decreased

previtellogenic egg chamber not present

mature egg not present

adult lethality

adult lethality


adult lethality

adult lethality


head appendages number decreased

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched


affected eggs/embryos/larvae


mature egg not present

tropharium structure irregular

egg chamber number decreased


affected eggs/embryos/larvae

adult lethality

egg chamber number decreased

mature egg not present

egg chamber stage sequence irregular

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

adult lethality immediately after eclosion


yolk late amount decreased

mature egg not present

egg production: number <50% of expected

egg production: number <50% of expected


abdomen not present

abdomen segment number decreased

thorax segment number decreased

thorax & abdomen segment number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


vitellogenic egg chamber late potentially degenerated

mature egg not present

larva number hatched none (< 10 %)


larva number hatched many (ca. 70-100 %)

adult lethality


affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality


head & thorax & abdomen segment shape irregular

head & thorax & abdomen segment random not present

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


antenna sometimes not present

head appendages position irregular

larva shape irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


egg chamber not present

mature egg not present

adult lethality


adult lethality

adult lethality

adult lethality


head capsule not present

head appendages position irregular

larva shape irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

egg production: number <50% of expected


adult lethality

adult lethality

eclosion not fulfilled (with pupal and adult features)


egg chamber mostly not present

mature egg not present

Potentially one extra nucleus is present in few egg chambers in different ovarioles.


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


abdominal odoriferous glands colour decreased

adult lethality

adult lethality


mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number no/almost no eggs

adult lethality


adult lethality

adult lethality


adult nutritional state potentially bad (cachectic)

adult nutritional state bad (cachectic)

adult lethality


embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

mature egg deposition 5 >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

egg production: number <50% of expected


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected


mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality


legs bent

head appendages position irregular

larva shape irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality


cuticle remnants with some identifiable segments

head capsule not present

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


head capsule not present

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

abdomen potentially not present

legs sometimes number decreased

head appendages potentially not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult nutritional state bad (cachectic)

adult lethality


larva constricted

Constriction often occurs between thorax and abdomen segments.

head & thorax segment random not present

head appendages position irregular

affected eggs/embryos/larvae

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva number hatched many (ca. 70-100 %)


vitellogenic egg chamber potentially degenerated

egg chamber mostly not present

mature egg not present


adult lethality

adult lethality

adult lethality


egg chamber mostly not present

mature egg not present

adult lethality


tropharium potentially shape irregular

mature egg mostly not present

ovary without detectable defects

Yolk in the vitellogenic egg chambers is not homogenous.


adult lethality

adult lethality


ovary without detectable defects

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


abdominal odoriferous glands potentially content decreased

adult lethality


larva cuticle thinner

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

adult nutritional state bad (cachectic)

adult lethality


adult lethality


larval musculature pattern irregular

affected eggs/embryos/larvae


head appendages mostly not present

abdomen segment number decreased

thorax segment number decreased

head capsule size decreased

head & thorax segment number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

adult lethality

head appendages number decreased

abdomen segment number decreased

thorax segment number decreased

head capsule size decreased

larva segment number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

ovary without detectable defects

vitellarium stage sequence irregular

yolk deposition irregular

previtellogenic egg chamber number increased

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality

mature egg not present

vitellogenic egg chamber late amount increased

yolk late amount decreased

egg production: number <50% of expected

adult lethality


previtellogenic egg chamber partially not present

previtellogenic egg chamber stage sequence irregular

previtellogenic egg chamber number decreased

mature egg not present

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality


larva size decreased

larva everted (inside-out)

larva fragmented

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

yolk sometimes amount decreased

mature egg not present

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

egg production: number <50% of expected


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

affected eggs/embryos/larvae

vitellogenic egg chamber late sometimes stage sequence irregular

yolk late sometimes amount decreased

ovary without detectable defects

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


larva partially everted (inside-out)

larva complete everted (inside-out)

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched


adult lethality

adult lethality


affected eggs/embryos/larvae

ovary without detectable defects

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected


vitellogenic egg chamber potentially partially degenerated

mature egg not present

adult nutritional state

adult lethality

adult lethality


adult lethality

adult lethality

adult lethality

adult lethality immediately after eclosion


larva number hatched many (ca. 70-100 %)


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (died as pupa)


adult lethality


larva number few (< 50 %) hatched

adult lethality


adult lethality

adult nutritional state potentially bad (cachectic)

adult lethality

adult lethality


adult lethality

adult lethality

eclosion not fulfilled (died as pupa)


adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

adult lethality


cuticle remnants strongly fragmented (cuticle crumbs)

affected eggs/embryos/larvae

adult lethality

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

eclosion not fulfilled (with pupal and adult features)


embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

mature egg not present

vitellogenic egg chamber late deposition irregular

vitellogenic egg chamber late number increased

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality


larval musculature partially not present

larval musculature thinner

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

eclosion not fulfilled (died as pupa)


tropharium potentially structure irregular

previtellogenic egg chamber structure irregular

vitellogenic egg chamber late not present

mature egg not present

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality

adult lethality


embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

adult lethality


larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality


embryo/egg with embryonic tissue, no cuticle, no fluorescence

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


antenna slightly orientation irregular

labrum mostly not present

labrum size decreased

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


head cuticle structure irregular

antenna shape irregular

labrum size decreased

head appendages orientation irregular

head potentially size decreased

posterior terminus shape irregular

head capsule structure irregular

affected eggs/embryos/larvae

adult lethality

larval musculature anterior not present

most larvae on slide appear like dying, but always starting anterior. potentially segmentation defect?

larval musculature thinner

affected eggs/embryos/larvae

vitellogenic egg chamber late sometimes degenerated

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality

adult lethality


larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality

eclosion not fulfilled (died as pupa)


adult lethality

affected eggs/embryos/larvae

adult lethality


adult lethality


posterior terminus potentially not present

antenna antennal flagellum not present

antenna scapus broader

legs femur potentially fused

legs tibiotarsus potentially fused

abdomen posterior constricted

legs size decreased

head capsule structure irregular

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

adult lethality

adult lethality

adult lethality


adult lethality

adult lethality

adult lethality


embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


abdomen segment number decreased

legs femur potentially fused

legs tibiotarsus potentially fused

head capsule size decreased

larva appendages shape irregular

posterior terminus not present

legs size shortened

head appendages structure irregular

larva segment number decreased

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)


embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality


abdominal odoriferous glands content split

adult lethality


adult nutritional state bad (cachectic)

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

larval musculature thinner

affected eggs/embryos/larvae

egg production: number <50% of expected

adult lethality


adult lethality

adult lethality


adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality immediately after eclosion


previtellogenic egg chamber potentially number increased

ovary without detectable defects

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

eclosion not fulfilled (died as pupa)


adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

adult lethality


adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

previtellogenic egg chamber position irregular

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality


embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

ovary without detectable defects

no fat body present, but ovaries look OK

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality


adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae


vitellogenic egg chamber late number decreased

vitellogenic egg chamber potentially number increased

yolk deposition irregular

mature egg not present

egg production: number no/almost no eggs

adult lethality


larva dorsal bent

larva bristle mostly not present

abdomen segment potentially number decreased

posterior terminus not present

legs podomer potentially fused

legs size decreased

head appendages potentially random not present

head appendages shape irregular

larva circumference decreased

abdomen circumference decreased

thorax size decreased

head size decreased

larva size decreased

cuticle remnants

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

abdominal odoriferous glands colour darker

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


abdominal odoriferous glands content decreased

abdominal odoriferous glands content darker

adult lethality

adult lethality

larva number hatched many (ca. 70-100 %)


thorax & abdomen segment fragmented

Fragmentation often separate thorax from abdomen.

thorax & abdomen segment constricted

Constriction occurs between thorax and abdomen segment

larva size thinner

Only few larvae showed this phenotype; maybe background.

thorax & abdomen segment not present

Sometimes 1st thorax segment is present but 2nd and 3rd segments are absent.

cuticle remnants with some identifiable segments

abdomen segment number decreased

head appendages shape irregular

head & thorax & abdomen segment shape irregular

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

adult lethality

adult lethality

adult lethality


adult lethality

adult lethality

adult lethality


adult lethality

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

adult lethality


head & thorax & abdomen segment random not present

abdomen segment number decreased

head appendages position irregular

larva complete everted (inside-out)

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


head appendages random not present

head appendages position irregular

head capsule partially not present

affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected


egg production: number no/almost no eggs

adult lethality

adult lethality


egg chamber mostly not present

mature egg not present

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality


adult lethality

adult nutritional state bad (cachectic)

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


thorax & abdomen segment dorsal pattern irregular

abdomen posterior circumference decreased

head appendages potentially random not present

legs size decreased

abdomen posterior dorsal bent

legs podomer potentially number decreased

head appendages shape irregular

larva complete everted (inside-out)

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

larva number hatched many (ca. 70-100 %)

adult lethality

adult lethality


larva shape irregular

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


ovariole potentially size decreased

vitellarium potentially size decreased

tropharium structure irregular

egg chamber potentially degenerated

egg chamber mostly not present

mature egg not present

adult lethality


adult lethality


adult lethality

tropharium structure irregular

egg chamber not present

mature egg not present

adult nutritional state potentially bad (cachectic)

adult lethality


cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


head capsule not present

head appendages random not present

head appendages position irregular

larva shape irregular

affected eggs/embryos/larvae

adult lethality

larval musculature random not present

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult nutritional state bad (cachectic)


embryo/egg not developed/not fertilized

affected eggs/embryos/larvae


adult lethality

adult lethality


affected eggs/embryos/larvae

tropharium structure irregular

tropharium potentially size decreased

vitellarium potentially size increased

egg chamber mostly not present

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

mature egg not present

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected


adult lethality

adult lethality

adult lethality


larva partially everted (inside-out)

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality


adult lethality

egg chamber mostly not present

mature egg not present


vertex posterior labrum bristle potentially not present

labrum shape irregular

labrum potentially size decreased

labrum posterior constricted

labrum potentially not present

antenna position irregular

antenna antennal flagellum not present

affected eggs/embryos/larvae

abdominal odoriferous glands potentially content darker

larval musculature pattern irregular

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected


vitellogenic egg chamber potentially degenerated

egg chamber mostly not present

mature egg not present

egg production: number no/almost no eggs


adult lethality

adult lethality

adult lethality

adult lethality


abdominal odoriferous glands content decreased

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

adult lethality

adult nutritional state bad (cachectic)

adult lethality


adult lethality

adult lethality

adult nutritional state bad (cachectic)

adult lethality

adult lethality


adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


abdominal odoriferous glands content darker


ovary without detectable defects

mature egg mostly not present


mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality


head appendages random not present

larva partially everted (inside-out)

head appendages position irregular

larva shape irregular

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


larva number hatched many (ca. 70-100 %)


adult lethality

adult lethality


head appendages random not present

head & thorax & abdomen segment shape irregular

abdomen segment number decreased

legs segment potentially fused

head appendages position irregular

head & thorax & abdomen segment random not present

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

larval musculature random not present

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


head appendages random not present

head appendages position irregular

head capsule size decreased

affected eggs/embryos/larvae

adult lethality

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


abdominal odoriferous glands potentially colour darker


larval musculature dorsal structure not present

Either dorsal muscles are absent or dorsal closure does not occur leading to lack of muscles dorsally.

Empty eggs but mostly the shell is not in typical shape.

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


eclosion not fulfilled (with pupal and adult features)


adult lethality

adult lethality

adult lethality

adult lethality


vitellogenic egg chamber mostly not present

previtellogenic egg chamber position irregular

mature egg not present

adult lethality

egg production: number no/almost no eggs

adult lethality


adult lethality

adult lethality

adult lethality


adult lethality


adult lethality

vitellogenic egg chamber potentially degenerated

previtellogenic egg chamber early not present

mature egg not present

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality


egg production: number <50% of expected


gena triplet bristle number irregular

vertex triplet vertex triplet ventral not present

head bristle number irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


thorax & abdomen segment number increased

Potentially mixed identity

abdomen potentially partially transformed

thorax potentially partially transformed

head appendages potentially partially transformed

legs not present

head appendages mostly not present

larva everted (inside-out)

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

adult lethality


larva number hatched none (< 10 %)

adult lethality

adult lethality


previtellogenic egg chamber potentially number increased

vitellogenic egg chamber not present

tropharium structure irregular

mature egg not present

adult lethality

adult lethality


head appendages unilateral sometimes number decreased

abdomen segment number decreased

thorax segment unilateral sometimes number decreased

head capsule size decreased

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva partially everted (inside-out)

larva everted (inside-out)

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality


labrum bent

head appendages number decreased

maxilla size decreased

head capsule size decreased

mandible not present

legs sometimes partially fused

abdomen segment number decreased

thorax segment number decreased

antenna not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

adult lethality

ovary without detectable defects

yolk late sometimes amount decreased

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

legs femur sometimes fused

antenna antennal flagellum sometimes not present

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

adult lethality

ovary without detectable defects

yolk late sometimes amount decreased

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality

egg production: number no/almost no eggs

adult lethality

adult lethality


adult lethality

egg production: number <50% of expected

adult lethality


mature egg not present

yolk partially amount decreased

egg chamber potentially degenerated

egg chamber stage sequence irregular

larva number hatched none (< 10 %)

egg production: number <50% of expected


adult lethality


affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality

adult lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


head appendages number decreased

antenna not present

labium not present

abdomen segment sometimes unilateral number decreased

legs size decreased

labrum not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult nutritional state potentially bad (cachectic)

adult lethality

adult lethality


adult lethality

larva number hatched none (< 10 %)


adult lethality

adult lethality


larva size decreased

larva potentially circumference decreased

larva everted (inside-out)

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

yolk late sometimes decreased

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected


larval musculature fluorescence decreased

affected eggs/embryos/larvae

adult lethality


yolk late amount decreased

larva number few (< 50 %) hatched


adult lethality immediately after eclosion


adult lethality

adult lethality

adult nutritional state bad (cachectic)


egg chamber potentially degenerated

egg chamber number decreased

tropharium structure irregular

mature egg not present


head capsule size decreased

prothorax not present

head appendages number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

adult lethality

affected eggs/embryos/larvae

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


head appendages position irregular

antenna size decreased

labrum size decreased

head capsule size decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)


larval musculature potentially thinner

affected eggs/embryos/larvae

adult lethality


head & thorax & abdomen appendages random shape irregular

head & thorax & abdomen segment random not present

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


abdominal odoriferous glands content darker

abdominal odoriferous glands content decreased

abdominal odoriferous glands potentially content not present

larva number hatched many (ca. 70-100 %)


vitellogenic egg chamber potentially degenerated

egg chamber number decreased

mature egg not present

vitellarium size shortened

abdominal odoriferous glands colour darker

previtellogenic egg chamber number decreased

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number no/almost no eggs