larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

pupa eclosion not fulfilled (died as pupa)

vitellarium size decreased

In some follicles there seem to be multilayerd follicle epithelium

wings shape irregular

tropharium size decreased

egg production: number <50% of expected

adult lethality

elytra partially blistered

eclosion not fulfilled (died as pupa)

previtellogenic egg chamber sometimes not present


larva number hatched none (< 10 %)

adult nutritional state bad (cachectic)

egg production: number no/almost no eggs

adult lethality

egg production: number <50% of expected

egg chamber differentiation irregular

egg chamber sometimes segmentation/division not present

May be in some cases due to preparation artifacts

vitellogenic egg chamber late not present

pronotum shape irregular

tropharium partially size decreased

elytra distal orientation irregular


larva number hatched many (ca. 70-100 %)

eclosion not fulfilled (with pupal and adult features)


adult lethality


egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

with embryonic tissue, no cuticle, no fluorescence

larval musculature differentiation incomplete

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality


affected eggs/embryos/larvae

cuticle remnants with unidentifyable segments

embryo/egg with embryonic tissue, no cuticle, no fluorescence

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality

adult lethality

mature egg deposition 3 5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

elytra anterior shape irregular

with dorsal median depression

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

wings shortened


affected eggs/embryos/larvae

adult lethality


legs irregular

legs segment fused

larval musculature fluorescence decreased

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larva number few (< 50 %) hatched

pupa eclosion not fulfilled (died as pupa)


adult lethality

larva lethality


embryo/egg condensed embryonic tissue, no cuticle, cns fluorescence


egg production: number no/almost no eggs

adult nutritional state bad (cachectic)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality late (L7)


affected eggs/embryos/larvae

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


abdomen dorsal not closed

random not present

cuticle remnants with some identifiable segments

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)


abdomen dorsal not closed

random not present

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)


larva lethality


affected eggs/embryos/larvae

head bristle pattern irregular

head irregular

head & thorax & abdomen bristle pattern irregular

larva number few (< 50 %) hatched

adult lethality

adult lethality

pronotum median surface irregular

ectopic, median, ap-oriented groove


larva number hatched none (< 10 %)

egg chamber potentially differentiation aborted

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (not completely eclosed)

pupal molt delayed (still larva)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

legs orientation irregular

antenna slightly orientation irregular

wings shortened

pupal molt delayed (still larva)

pupal molt delayed (still larva)


egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number few (< 50 %) hatched

eclosion not fulfilled (with pupal and adult features)

ovary without detectable defects

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected


larva number hatched many (ca. 70-100 %)

adult lethality immediately after eclosion


adult lethality

adult lethality


egg chamber sometimes potentially degenerated

egg chamber sometimes potentially fused

egg chamber structure irregular

vitellogenic egg chamber late not present

adult nutritional state potentially bad (cachectic)

larva number hatched none (< 10 %)

mature egg not present

yolk irregular

adult lethality

ovary size decreased

ovariole not present

adult cuticle colour darker

adult lethality

adult nutritional state bad (cachectic)


adult lethality


larva number hatched none (< 10 %)

previtellogenic egg chamber potentially not present

vitellogenic egg chamber not present

egg production: number <50% of expected

adult lethality

pupa eclosion not fulfilled (died as pupa)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality


head capsule size decreased

legs femur shortened

legs irregular

head appendages shortened

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

head size decreased

pupa lethality as pupa

elytra blistered

pupal molt not fulfilled (died as prepupa)


adult lethality

eclosion not fulfilled (died as pupa)


larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality

adult nutritional state bad (cachectic)

egg production: number <50% of expected

larva lethality late (L7)

died with yellowish feces at posterior

pupal molt delayed (still larva)

larva lethality


larva number hatched none (< 10 %)

adult nutritional state bad (cachectic)

adult lethality

egg production: number <50% of expected

pupa seta number decreased

pupa bristle number decreased

eclosion not fulfilled (died as pupa)


cuticle remnants with some identifiable segments

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

larva segment random not present

cuticle partially remaining

cuticle remnants with unidentifyable segments

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched

adult lethality

larva lethality


egg irregular

egg shell number increased

larva cuticle fluorescence decreased

condensed embryonic tissue, no cuticle, cns fluorescence

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number hatched none (< 10 %)

yolk amount decreased

previtellogenic egg chamber potentially number decreased


affected eggs/embryos/larvae

larval musculature fluorescence decreased

embryo/egg

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched

ovary without detectable defects

egg production: number <50% of expected

eclosion not fulfilled (with pupal and adult features)

tropharium size decreased

vitellarium not present

hindwing potentially folding irregular

elytra shape rounded

ovary size decreased

adult nutritional state bad (cachectic)

larva lethality


larva number hatched many (ca. 70-100 %)

adult lethality


affected eggs/embryos/larvae

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

embryo/egg

adult lethality


motility irregular

animals avoid the middle of the vial and ca. 50% play dead, but are not

larva lethality


adult lethality

larva lethality

adult lethality


elytra surface irregular

elytra ventral not closed


larva number few (< 50 %) hatched

mature egg deposition 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality

pupa lethality as pupa

elytra necrotic

pupation not fulfilled (died as prepupa)

eclosion not fulfilled (died as pupa)

larva lethality early (L5/6)

larva number hatched none (< 10 %)


yolk irregular

mature egg not present

vitellogenic egg chamber number increased

egg chamber number decreased

adult lethality

egg production: number <50% of expected

adult lethality immediately after eclosion

larva lethality late (L7)

pupal molt not fulfilled (died as pupa)

pupal molt delayed (still larva)

larva lethality


adult lethality

adult nutritional state bad (cachectic)

adult lethality

larva lethality late (L7)


pupation


larva number hatched none (< 10 %)

mature egg not present

wings not closed

adult lethality

elytra size shortened

antenna size shortened

pupa complete shortened


egg chamber irregular

adult nutritional state bad (cachectic)

adult lethality

larva lethality late (L7)

larva lethality late (L7)

larva lethality early (L5/6)


larva lethality


thorax & abdomen everted (inside-out)

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

pupa eclosion not fulfilled (died as pupa)

elytra blistered


larva number hatched none (< 10 %)

egg chamber number decreased

vitellarium size decreased

adult lethality

adult lethality

eclosion delayed (still pupa)

eclosion delayed (still pupa)

pupa lethality as pupa

antenna shortened

larva lethality early (L5/6)


larva number hatched none (< 10 %)

adult lethality

adult lethality

adult lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

embryo/egg

adult lethality

adult nutritional state bad (cachectic)

pupation delayed (still larva)


adult lethality

pupation


adult lethality

adult lethality

larva lethality


adult lethality

adult lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality

adult lethality

adult lethality

adult lethality


larva lethality late (L7)


affected eggs/embryos/larvae

affected eggs/embryos/larvae

abdomen musculature partially not present

embryo/egg

larva number few (< 50 %) hatched

adult lethality

larva lethality early (L5/6)


affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number hatched none (< 10 %)

elytra orientation irregular

hindwing shape irregular


larva number few (< 50 %) hatched

larva lethality early (L5/6)


adult lethality

pupation

larva lethality early (L5/6)


affected eggs/embryos/larvae

egg shell surface thinner

embryo/egg not developed/not fertilized

egg shell robustness decreased

Fixation with standard procedure not possible. Eggs are destroyed during clorization.

egg shell robustness decreased

All eggs diluted during clorization

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

adult lethality

adult lethality

larva lethality


egg production: number no/almost no eggs

adult nutritional state bad (cachectic)

larva lethality


adult nutritional state bad (cachectic)

pupal molt not fulfilled (died as prepupa)

pupation delayed (still prepupa)

larva lethality early (L5/6)


larva number hatched none (< 10 %)

adult lethality

larva lethality


abdomen dorsal irregular

abdomen everted (inside-out)

thorax complete everted (inside-out)

thorax & abdomen everted (inside-out)

larva number few (< 50 %) hatched

adult lethality


tropharium potentially degenerated

tropharium structure irregular

vitellarium size decreased

egg chamber not present

eclosion not fulfilled (not completely eclosed)

wings not closed

antenna orientation irregular

wings shortened

head potentially anteroposterior-axis shortened


larva lethality


adult lethality

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva everted (inside-out)

embryo/egg not developed/not fertilized

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

embryo/egg

larva number few (< 50 %) hatched

wings proximal blistered

larva lethality

pupa lethality as pupa

wings shortened

eclosion not fulfilled (died as pupa)


larva number hatched none (< 10 %)

vitellogenic egg chamber not present

adult lethality

pupal (?) urogomphi are visible posterior through larval cuticle


adult lethality

larva lethality early (L5/6)


larva number hatched none (< 10 %)

adult nutritional state bad (cachectic)

adult lethality

pupation delayed (still prepupa)

larva lethality early (L5/6)


pupation

larva lethality early (L5/6)


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

embryo/egg not developed/not fertilized

adult lethality

adult lethality

pupation


affected eggs/embryos/larvae

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched

pupation delayed (still larva)


adult lethality


affected eggs/embryos/larvae

head & thorax partially not present

adult lethality

eclosion delayed (still larva)

pupal molt delayed (still larva)

pupation delayed (still larva)

larva lethality early (L5/6)


Almost no eggs present, but those embryos which are there seem to hatch.

adult lethality

adult nutritional state bad (cachectic)

adult lethality

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality


affected eggs/embryos/larvae

abdomen partially everted (inside-out)

head irregular

abdomen constricted

thorax mostly not present

head partially not present

head & thorax & abdomen fragmented

larva number few (< 50 %) hatched

adult lethality

abdominal odoriferous glands partially colour darker

abdominal odoriferous glands colour darker


affected eggs/embryos/larvae

thorax partially constricted

affected eggs/embryos/larvae

embryo/egg

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number hatched none (< 10 %)

motility irregular

Legs are correctly formed, but can't be streched out. Joints between femur and tibia are not moveable.

larva lethality early (L5/6)


affected eggs/embryos/larvae

affected eggs/embryos/larvae

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

pupation

larva lethality

eclosion not fulfilled (with pupal and adult features)


larva number hatched none (< 10 %)

adult nutritional state bad (cachectic)

pupa lethality as pupa

pupation not fulfilled (died with pupal and larval features)

eclosion not fulfilled (died as pupa)


egg chamber shape rounded

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

antenna shortened

hindwing necrotic

female genital lobes size decreased

elytra size decreased

head size decreased

eclosion not fulfilled (died as pupa)

larva lethality late (L7)


affected eggs/embryos/larvae

tropharium structure irregular

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

egg chamber not present

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

ovipositor protruding

adult lethality

adult lethality

longitudinal dorsal thoracic muscles partially not present

elytra shape rounded

elytra shortened

thorax orientation irregular

head orientation irregular

adult lethality


affected eggs/embryos/larvae

head capsule size decreased

head capsule shape irregular

leg musculature sometimes not present

larva number hatched many (ca. 70-100 %)

adult lethality

larva lethality early (L5/6)


larva number hatched none (< 10 %)

adult lethality

adult nutritional state bad (cachectic)

adult lethality

larva lethality late (L7)

pupation delayed (still larva)

larva lethality early (L5/6)


adult lethality

adult lethality immediately after eclosion

adult lethality

pupation


adult lethality

adult lethality

larva lethality


larva lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

embryo/egg

adult lethality

pupa eclosion not fulfilled (died as pupa)


vitellarium shape thinner

larva number hatched none (< 10 %)

egg chamber complete not present

adult lethality

vitellarium not present

tropharium mostly not present

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

egg production: number no/almost no eggs

adult lethality


urogomphi potentially transformed

posterior terminus irregular

abdomen segment number # of remaining segments  increased

Most animals die as L2 larvae

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

pupal molt not fulfilled (with pupal and adult features)

pupal molt not fulfilled (died as pupa)

oblique dorsal thoracic muscles broader

oblique dorsal thoracic muscles sometimes orientation irregular

female genital lobes potentially duplicated

oblique dorsal thoracic muscles shortened

female genital lobes shortened

pupal molt delayed (still prepupa)

larva lethality


larva number hatched none (< 10 %)

adult nutritional state bad (cachectic)

adult lethality

larva lethality late (L7)

larva lethality late (L7)

larva lethality early (L5/6)


pupation

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

adult lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality

eclosion not fulfilled (died as pupa)


abdomen partially # of remaining segments  not present

head partially not present

affected eggs/embryos/larvae

thorax segment partially not present

head bristle pattern irregular

head & thorax & abdomen partially not present


adult lethality

pupation precocious


adult nutritional state potentially bad (cachectic)

egg chamber not present

tropharium size decreased

larva lethality late (L7)

larva lethality late (L7)

pupation delayed (still larva)

larva lethality


adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

larva lethality late (L7)

larva lethality early (L5/6)


affected eggs/embryos/larvae

larval musculature fluorescence decreased


vitellarium irregular

larva number hatched none (< 10 %)

egg chamber number increased

adult lethality

egg production: number <50% of expected

adult lethality

larva lethality

adult lethality


head partially not present

head not present

affected eggs/embryos/larvae

thorax partially not present

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

larva number hatched many (ca. 70-100 %)

adult lethality

adult lethality

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

pupation delayed (still larva)

larva lethality


adult nutritional state bad (cachectic)

larva lethality early (L5/6)


affected eggs/embryos/larvae

head appendages partially not present

thorax partially not present

head capsule size decreased

head & thorax partially not present

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number few (< 50 %) hatched

adult lethality

larva lethality


larva number hatched none (< 10 %)

adult lethality

tropharium size increased

egg production: number <50% of expected

larva lethality early (L5/6)


head appendages mostly not present

head capsule shape irregular

cuticle remnants without visible segmentation, differentiation or axis

affected eggs/embryos/larvae

larval musculature partially not present

larval musculature partially rounded

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

gnathal appendages partially shape irregular

gnathal appendages partially position irregular

gnathal appendages partially not present

head capsule shape irregular

abdomen musculature irregular

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched

adult lethality

pupation


larva lethality late (L7)

pupation delayed (still larva)

larva lethality


vitellogenic egg chamber potentially degenerated

vitellogenic egg chamber mostly not present

previtellogenic egg chamber not present

egg production: number no/almost no eggs

adult lethality

adult lethality immediately after eclosion

wings shortened

antenna shortened

larva lethality late (L7)

larva lethality late (L7)

pupal molt delayed (still larva)

pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still prepupa)

eclosion not fulfilled (died as pupa)

larva lethality


affected eggs/embryos/larvae

legs sometimes everted (inside-out)

abdomen constricted

abdomen dorsoventral-axis shortened

affected eggs/embryos/larvae

head & thorax & abdomen partially not present

legs pretarsus not present

antenna not present

embryo/egg

abdomen musculature partially not present

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched

adult lethality

adult lethality

eclosion not fulfilled (with pupal and adult features)

eclosion not fulfilled (died as pupa)


larva number hatched none (< 10 %)

adult lethality

egg production: number <50% of expected

adult lethality

pupa eclosion not fulfilled (died as pupa)

larva lethality


cuticle remnants with some identifiable segments

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number hatched none (< 10 %)

egg chamber mostly not present

egg production: number <50% of expected

motility decreased

elytra not closed

adult lethality

adult nutritional state bad (cachectic)

elytra partially blistered


larva number hatched none (< 10 %)

adult lethality


larva number hatched many (ca. 70-100 %)


pupation


adult lethality

adult nutritional state bad (cachectic)

adult lethality

larva lethality


embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult nutritional state bad (cachectic)

pupa lethality as pupa

elytra shortened

pupation not fulfilled (died as prepupa)

larva lethality early (L5/6)


labrum size decreased

mandible potentially transformed

mandible not present

maxilla number increased

affected eggs/embryos/larvae

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality


larval musculature fluorescence not present

larval musculature dorsal shape irregular

larval musculature ventral shape irregular

larval musculature fluorescence decreased

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number few (< 50 %) hatched


egg production: number no/almost no eggs

adult lethality

adult lethality

larva lethality early (L5/6)


affected eggs/embryos/larvae

embryo/egg

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

eclosion not fulfilled (died as pupa)

larva lethality late (L7)


affected eggs/embryos/larvae

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

ovariole mostly not present

ovary size decreased

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

pupation delayed (still larva)

1 larva killed


adult lethality

adult lethality

larva lethality


larva number hatched none (< 10 %)

adult nutritional state bad (cachectic)

adult lethality

wings blistered

pupa lethality as pupa

mature egg not present

tropharium structure irregular

egg chamber number decreased


affected eggs/embryos/larvae

egg production: number <50% of expected

Egg number seems to be a little bit reduced, maybe 60-70% of expected number.


vitellogenic egg chamber elongated

egg production: number <50% of expected

adult lethality


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

adult lethality

larva lethality

larva anterior constricted

larvae show constrictions only between the head and the thorax

head & thorax partially not present

cuticle remnants without visible segmentation, differentiation or axis

larva number few (< 50 %) hatched


pupal molt not fulfilled (died as L7 or prepupa)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

larva number hatched none (< 10 %)

adult lethality

adult nutritional state bad (cachectic)

adult lethality


larva lethality

eclosion not fulfilled (died as pupa)

adult lethality


pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

larva lethality late (L7)

previtellogenic egg chamber number decreased

vitellogenic egg chamber not present

vitellarium shortened

larva number hatched none (< 10 %)

mature egg not present

adult lethality


pupal molt delayed (still prepupa)

larva lethality

head appendages shape irregular

thorax not present

legs trochanter not present

legs coxa not present

legs tibiotarsus fused

legs femur fused

legs segment shortened

abdomen shape irregular

larval musculature sometimes thinner

larval musculature partially not present

larval musculature mostly orientation irregular

larva number few (< 50 %) hatched

eclosion not fulfilled (with pupal and adult features)


pupal molt delayed (still larva)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

wings complete not present

antenna rounded

eclosion delayed (still pupa)

pupal molt delayed (still larva)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

larva lethality

larva number hatched none (< 10 %)

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

pupa eclosion not fulfilled (died as pupa)


eclosion not fulfilled (died as pupa)

larva lethality late (L7)

pupal molt delayed (still larva)

wings blistered

pupal molt not fulfilled (died as pupa)

larva lethality

larva number hatched none (< 10 %)

mature egg deposition 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


larva lethality

head appendages random not present

prothoracic leg sometimes not present

head capsule mostly not present

head & thorax irregular

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number few (< 50 %) hatched


pupal molt delayed (still larva)

eclosion not fulfilled (died as pupa)

larva lethality late (L7)

head bent

pupal molt not fulfilled (died as pupa)

wings size decreased

antenna shortened

larva lethality

egg chamber fused

egg chamber number decreased

larva number hatched none (< 10 %)

mature egg not present

adult lethality


larva lethality

larva number hatched many (ca. 70-100 %)


adult lethality

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality


eclosion delayed (still prepupa)

pupal molt delayed (still larva)

larva number hatched none (< 10 %)

vitellarium mostly not present

adult lethality


adult lethality

larva lethality

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)


adult lethality

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

adult lethality


vitellarium shortened

previtellogenic egg chamber not present

prooocytes not present

egg production: number <50% of expected

adult lethality

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

larva lethality

larva shape irregular

cuticle remnants with unidentifyable segments

larva number hatched none (< 10 %)


larva lethality

larva number hatched none (< 10 %)

adult lethality

adult nutritional state bad (cachectic)

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality


pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

larva lethality late (L7)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

larva lethality

larva number hatched none (< 10 %)

adult lethality

adult nutritional state bad (cachectic)

egg production: number <50% of expected


eclosion not fulfilled (died as pupa)

adult lethality

eclosion delayed (still pupa)

larva lethality

adult lethality


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

adult lethality

pupal molt delayed (still prepupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


egg production: number no/almost no eggs

adult nutritional state bad (cachectic)

eclosion not fulfilled (died as pupa)

adult lethality

eclosion delayed (still pupa)

larva lethality

larva number hatched none (< 10 %)

vitellogenic egg chamber not present

vitellarium size decreased

mature egg not present

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality

adult lethality

eclosion delayed (still pupa)

larva lethality

larva number few (< 50 %) hatched

adult lethality


adult lethality

larva lethality

larva number few (< 50 %) hatched


eclosion not fulfilled (died as pupa)

adult lethality

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


larva lethality


larva lethality late (L7)

eclosion delayed (still prepupa)

eclosion delayed (still larva)

pupal molt delayed (still larva)

larva lethality

pupal molt delayed (still prepupa)

vitellarium partially thinner

vitellogenic egg chamber sometimes degenerated

egg chamber number decreased

larva number hatched none (< 10 %)

mature egg not present

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

larva lethality


larva lethality

larva number few (< 50 %) hatched

adult lethality immediately after eclosion


larva lethality

adult lethality


adult lethality

larva lethality


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva number few (< 50 %) hatched


adult lethality

eclosion delayed (still pupa)

pupal molt delayed (still larva)


adult lethality

eclosion not fulfilled (not completely eclosed)

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

hindwing blistered

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality


eclosion not fulfilled (died as pupa)

abdomen gin-traps number increased

female genital lobes rounded

eclosion delayed (still pupa)

abdomen gin-traps structure irregular

abdomen elongated

eclosion not fulfilled (died as pupa)

larva lethality

larva cuticle duplicated

larva number hatched none (< 10 %)

mature egg deposition 4 5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality


eclosion not fulfilled (died as pupa)

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

adult lethality


adult lethality

adult lethality

adult lethality

adult lethality

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

abdominal odoriferous glands shape irregular

fertility after larval injection decreased (no living offspring)

abdominal odoriferous glands partially content not present

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

ovary without detectable defects

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult nutritional state potentially bad (cachectic)

larva lethality

ovary without detectable defects

tropharium size shortened

sometimes too many cells between the egg-chambers

egg chamber shape irregular

larva number hatched none (< 10 %)

mature egg not present

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (with pupal and adult features)

labium not present

head & thorax segment partially not present

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larval musculature pattern irregular

larval musculature fluorescence decreased

larva number hatched many (ca. 70-100 %)


pupal molt not fulfilled (died as prepupa)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still prepupa)

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

egg chamber sometimes shape rounded

vitellogenic egg chamber number decreased

previtellogenic egg chamber mostly not present

mature egg not present

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality

pupa eclosion not fulfilled (died as pupa)


adult lethality

adult lethality


pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

larva lethality

larva number hatched none (< 10 %)

adult nutritional state bad (cachectic)


mature egg not present

egg production: number <50% of expected

larva lethality late (L7)

larva lethality

gnathal appendages sometimes remaining

abdomen not present

thorax segment partially not present

thorax & abdomen shortened

procephalic appendages sometimes remaining

cuticle remnants with some identifiable segments

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

larval musculature irregular

larva number few (< 50 %) hatched

adult lethality

abdominal odoriferous glands colour decreased

adult lethality

adult lethality


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

larva lethality


eclosion delayed (still pupa)

eclosion not fulfilled (died as pupa)

adult lethality

larva lethality

head appendages partially not present

abdomen segment number decreased

thorax segment partially not present

head appendages position irregular

head & thorax & abdomen irregular

appendages partially remaining

larval musculature irregular

larva number few (< 50 %) hatched

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number hatched none (< 10 %)


eclosion delayed (still pupa)

eclosion not fulfilled (died as pupa)

pupal molt not fulfilled (died as pupa)

pupal molt not fulfilled (died with pupal and larval features)

wings shortened

pupal molt delayed (still prepupa)

larva lethality

tropharium irregular

previtellogenic egg chamber number decreased

vitellogenic egg chamber not present

mature egg not present

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality

eclosion not fulfilled (with pupal and adult features)

larva cuticle duplicated

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larval musculature irregular

larva number hatched none (< 10 %)


eclosion not fulfilled (died as pupa)

adult lethality

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality


eclosion not fulfilled (died as pupa)

adult lethality

adult lethality

eclosion delayed (still pupa)

pupal molt delayed (still larva)

adult lethality


larva lethality

appendages partially remaining

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality

adult lethality


larva lethality

larva number few (< 50 %) hatched

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

adult lethality


larva lethality

larval musculature irregular

larva number few (< 50 %) hatched

adult lethality


eclosion not fulfilled (died as pupa)

pupal molt delayed (still prepupa)

appendages partially remaining

larva number hatched none (< 10 %)

adult lethality

larva dorsal not closed

head appendages shape irregular

head & thorax & abdomen segment partially not present

cuticle remnants with unidentifyable segments

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

larva lethality

larva number hatched many (ca. 70-100 %)

adult lethality


pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still prepupa)

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

larva lethality

larva number few (< 50 %) hatched

mature egg deposition 3 5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality

adult lethality

pupa eclosion not fulfilled (died as pupa)


pupal molt delayed (still prepupa)

larva lethality

larva everted (inside-out)

cuticle remnants upended (inside-outed)

embryo/egg not developed/not fertilized

vitellogenic egg chamber number decreased

tropharium irregular

larva number hatched none (< 10 %)

mature egg sometimes not present

egg production: number <50% of expected


larva lethality early (L5/6)

embryo/egg not developed/not fertilized

ovary without detectable defects

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

egg production: number <50% of expected


larva lethality early (L5/6)

thorax partially fused

larva appendages sometimes fused

larva appendages irregular

head & thorax & abdomen partially not present

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched


pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

delayed (still prepupa)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality late (L7)

previtellogenic egg chamber sometimes segmentation/division not present

vitellogenic egg chamber not present

egg production: number no/almost no eggs

egg production: number <50% of expected

adult lethality


adult lethality

adult nutritional state bad (cachectic)

motility decreased

adult lethality

larva lethality early (L5/6)

embryo/egg not developed/not fertilized

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


larva lethality early (L5/6)

adult lethality

adult lethality


eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

wings shortened

pupal molt not fulfilled (died with pupal and larval features)

larva lethality early (L5/6)

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality early (L5/6)

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

larva lethality early (L5/6)

thorax musculature partially thinner

abdomen musculature dorsal shortened

larval musculature partially not present

ovary without detectable defects

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

egg production: number <50% of expected


ovary without detectable defects

egg production: number <50% of expected

motility decreased

oblique dorsal thoracic muscles partially fragmented

oblique dorsal thoracic muscles thinner

abdomen constricted

thorax partially not present

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

embryo/egg with embryonic tissue, no cuticle, no fluorescence

embryo/egg not developed/not fertilized

larva number hatched many (ca. 70-100 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


larva lethality early (L5/6)

adult lethality


eclosion not fulfilled (died as pupa)

legs orientation irregular

wings blistered

female genital lobes rounded

oblique dorsal thoracic muscles fragmented

wings shortened

antenna orientation irregular

pupal molt not fulfilled (died as pupa)

larva lethality early (L5/6)

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

adult lethality


tropharium shape irregular

tropharium size shortened

vitellarium not present

mature egg not present

egg chamber not present

larva lethality early (L5/6)

larva number hatched none (< 10 %)

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

adult lethality


pupation delayed (still larva)

pupation not fulfilled (died as prepupa)

larva lethality early (L5/6)

adult lethality

larva number few (< 50 %) hatched

adult lethality


larva lethality late (L7)

larva lethality early (L5/6)

adult lethality


larva lethality early (L5/6)

ovary without detectable defects

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality


larva lethality early (L5/6)


adult lethality

larva lethality early (L5/6)

adult lethality


larva lethality late (L7)

larva lethality early (L5/6)

larva number hatched none (< 10 %)

adult lethality

adult lethality


larva lethality early (L5/6)

labrum size decreased

labium not present

prothorax not present

maxilla not present

head & thorax partially not present

adult lethality

adult lethality


adult lethality

pupal molt not fulfilled (died as prepupa)

elytra shortened

elytra not closed

adult lethality immediately after eclosion

eclosion not fulfilled (not completely eclosed)

pupal molt delayed (still prepupa)

wings shortened

larva lethality early (L5/6)

adult lethality


adult lethality

adult lethality


eclosion not fulfilled (died as pupa)

legs shape irregular

wings shortened

wings blistered

antenna shortened

pupal molt not fulfilled (died as pupa)

larva lethality early (L5/6)

egg chamber not present

tropharium structure irregular

vitellarium broader

vitellarium shortened

adult lethality

egg production: number <50% of expected

adult lethality

pupa eclosion not fulfilled (died as pupa)


pupal molt not fulfilled (died as L7 or prepupa)

pupal molt not fulfilled (died as pupa)

wings not closed

pupal molt not fulfilled (died with pupal and larval features)

wings shortened

antenna shortened

pupal molt delayed (still larva)

larva number hatched none (< 10 %)

previtellogenic egg chamber rounded

previtellogenic egg chamber number decreased

vitellogenic egg chamber not present

uterus size increased

ovary size decreased

egg production: number no/almost no eggs

adult lethality

adult lethality


legs necrotic

wings necrotic

pupal molt not fulfilled (died as pupa)

larva lethality early (L5/6)

larva number hatched none (< 10 %)

adult nutritional state bad (cachectic)

egg production: number <50% of expected

adult lethality


eclosion not fulfilled (with pupal and adult features)

oblique dorsal thoracic muscles thinner

longitudinal dorsal thoracic muscles number increased

wings not closed

larva lethality late (L7)

oblique dorsal thoracic muscles number increased

wings size shortened

larva lethality early (L5/6)

previtellogenic egg chamber shape rounded

vitellogenic egg chamber not present

vitellarium shortened

larva number hatched none (< 10 %)

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

egg production: number <50% of expected

adult lethality

adult lethality


larva lethality early (L5/6)

adult lethality


ovary without detectable defects

egg production: number <50% of expected

larva lethality early (L5/6)

cuticle remnants with some identifiable segments

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


adult lethality

larva number hatched many (ca. 70-100 %)

adult lethality


larva lethality

embryo/egg with embryonic tissue, no cuticle, no fluorescence

ovary without detectable defects

egg production: number <50% of expected


adult lethality

larva lethality early (L5/6)

adult lethality

adult lethality


adult lethality

adult lethality

adult lethality

elytra not closed

adult lethality immediately after eclosion

eclosion not fulfilled (not completely eclosed)

larva lethality early (L5/6)


eclosion not fulfilled (died as pupa)

pupal molt not fulfilled (died as L7 or prepupa)

oblique dorsal thoracic muscles shortened

wings shortened

antenna orientation irregular

pupal molt delayed (still larva)

adult lethality

larva number hatched none (< 10 %)

adult lethality

egg production: number <50% of expected

adult lethality


pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

larva number hatched none (< 10 %)

adult lethality

egg production: number <50% of expected

pupa eclosion not fulfilled (died as pupa)


adult lethality

adult lethality

motility decreased

larva lethality early (L5/6)

mature egg deposition 5 >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


eclosion not fulfilled (died as pupa)

pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still prepupa)

cuticle remnants with some identifiable segments

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

vitellogenic egg chamber number decreased

previtellogenic egg chamber not present

vitellogenic egg chamber sometimes shape constricted

larva number hatched none (< 10 %)

adult lethality


wings not closed

legs orientation irregular

wings not closed

antenna orientation irregular

larva number hatched none (< 10 %)

adult lethality


larva lethality early (L5/6)

larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality immediately after eclosion

adult lethality

adult lethality


larva lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

delayed (still larva)

larva lethality late (L7)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

larva number hatched none (< 10 %)

adult lethality

adult lethality


pupal molt not fulfilled (died with pupal and larval features)

ovary without detectable defects

one male found

embryo/egg not developed/not fertilized

egg rounded

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


larva lethality early (L5/6)

larval musculature thinner

larval musculature pattern irregular

larval musculature fluorescence decreased


larva lethality early (L5/6)

adult lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


ovary without detectable defects

egg production: number <50% of expected

ovary without detectable defects

egg production: number <50% of expected

adult lethality


elytra not closed

wings not closed

egg production: number <50% of expected

adult nutritional state bad (cachectic)

eclosion not fulfilled (not completely eclosed)

adult lethality

adult lethality


adult lethality


pupal molt not fulfilled (died as L7 or prepupa)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

larva number hatched none (< 10 %)

adult lethality


larva lethality early (L5/6)


mature egg degenerated

tropharium shortened

tropharium shape irregular

larva lethality early (L5/6)

previtellogenic egg chamber sometimes segmentation/division not present

previtellogenic egg chamber shape irregular

vitellogenic egg chamber not present

egg production: number no/almost no eggs

previtellogenic egg chamber number decreased

larva number few (< 50 %) hatched


larva lethality early (L5/6)

larva lethality

adult lethality

eclosion not fulfilled (with pupal and adult features)

adult lethality

adult lethality


larva lethality early (L5/6)

larva lethality

adult lethality

adult lethality

adult lethality


larva lethality early (L5/6)

adult lethality


larva lethality early (L5/6)

abdomen shortened

adult lethality


larva lethality early (L5/6)

abdomen shape irregular

thorax partially not present

head partially not present

larva mostly everted (inside-out)

cuticle remnants without visible segmentation, differentiation or axis

larval musculature irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larva number few (< 50 %) hatched

adult lethality


tropharium shape irregular

vitellarium not present

egg chamber not present

mature egg not present

larva number hatched none (< 10 %)

adult lethality

adult lethality


elytra shortened

elytra not closed

adult lethality

adult nutritional state bad (cachectic)

motility decreased

wings size shortened

pupation delayed (still larva)

abdominal odoriferous glands mostly content not present

abdominal odoriferous glands shape irregular

adult lethality

median groove shape irregular

the animals bear a small but very distinct dent at 2/3th of the length of the median groove on the metabasisternum

elytra rounded

hindwing potentially blistered

hindwing folding irregular

elytra shape irregular

larva number hatched none (< 10 %)

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

egg production: number <50% of expected

larva segment potentially fused

head & thorax segment partially not present

head decreased

larva segment partially not present

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (died as pupa)


larva lethality early (L5/6)

larva number hatched many (ca. 70-100 %)


ovary without detectable defects

egg production: number <50% of expected

adult lethality

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

adult lethality


larva lethality early (L5/6)

larval musculature partially not present


adult lethality

larva lethality early (L5/6)


pupal molt not fulfilled (died as prepupa)

eclosion not fulfilled (died as pupa)

pupal molt not fulfilled (died as pupa)

pupal molt delayed (still prepupa)

wings necrotic

pupal molt not fulfilled (died as L7 or prepupa)

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

adult lethality

adult lethality immediately after eclosion


pupal molt delayed (still prepupa)

legs partially shape irregular

egg shell robustness decreased

egg shell robustness decreased

larva number hatched none (< 10 %)

adult lethality


pupation delayed (still prepupa)

larva lethality early (L5/6)

larva number hatched none (< 10 %)

vitellogenic egg chamber not present

previtellogenic egg chamber mostly not present

vitellarium shortened

egg production: number no/almost no eggs

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

larva lethality early (L5/6)

adult lethality


larva lethality early (L5/6)

with embryonic tissue, no cuticle, but muscle fluorescence

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

adult lethality

adult lethality


adult lethality

abdomen constricted

adult lethality

pupa eclosion not fulfilled (died as pupa)


larva lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

adult lethality


eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

wings not present

pupal molt not fulfilled (died as pupa)

larva lethality early (L5/6)

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult nutritional state bad (cachectic)

adult lethality


pupation delayed (still prepupa)

pupation delayed (still larva)

larva lethality early (L5/6)

larva number hatched none (< 10 %)

adult nutritional state bad (cachectic)

adult lethality


ovary without detectable defects

egg production: number >150% of expected

larva lethality early (L5/6)

with embryonic tissue, no cuticle, no fluorescence

larval musculature irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

thorax & abdomen partially not present

cuticle remnants with some identifiable segments

larva number hatched none (< 10 %)

adult lethality


adult lethality

larva lethality early (L5/6)

larva number hatched many (ca. 70-100 %)


larva lethality early (L5/6)

adult lethality


adult lethality

ovary without detectable defects

egg production: number <50% of expected

larva lethality early (L5/6)

larva number hatched none (< 10 %)

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected


larva lethality early (L5/6)

adult lethality

adult lethality

adult lethality


pupal molt delayed (still prepupa)

larva lethality early (L5/6)


ovary without detectable defects

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

larva lethality early (L5/6)

legs tibiotarsus partially shortened

legs segment partially distal fused

adult lethality

labrum size decreased

mandible potentially transformed

mandible not present

maxilla number increased

affected eggs/embryos/larvae

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality


pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still prepupa)

larva lethality

larva number hatched none (< 10 %)

adult nutritional state bad (cachectic)


larva lethality late (L7)

pupal molt

pupation delayed (still larva)

larva number hatched none (< 10 %)

abdominal odoriferous glands colour opaque

egg chamber not present

adult lethality


larva lethality early (L5/6)

abdomen partially remaining

thorax partially remaining

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

larva number few (< 50 %) hatched

larval musculature fluorescence not present

embryo/egg not developed/not fertilized

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larval musculature irregular

adult lethality


adult lethality

ovary without detectable defects

egg production: number <50% of expected

adult nutritional state bad (cachectic)

larva lethality early (L5/6)

larva number hatched none (< 10 %)

embryo/egg not developed/not fertilized

egg production: number <50% of expected

adult lethality


eclosion delayed (still pupa)

adult lethality immediately after eclosion

pupal molt delayed (still larva)

larva lethality early (L5/6)

adult lethality

eclosion not fulfilled (with pupal and adult features)


prothoracic odoriferous glands partially colour darker

larva lethality

adult lethality


larva lethality

adult lethality


wings shortened

pupa lethality during eclosion

elytra shortened

eclosion not fulfilled (died as pupa)

larva lethality early (L5/6)

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


larva lethality late (L7)

larva lethality early (L5/6)

larva number hatched none (< 10 %)

adult lethality

egg production: number no/almost no eggs

adult lethality


larva lethality early (L5/6)

adult lethality

egg production: number <50% of expected

adult lethality

pupa eclosion not fulfilled (died as pupa)


adult lethality

tropharium shape irregular

tropharium shortened

egg production: number <50% of expected

larva lethality

abdomen cuticle irregular

head & thorax partially not present

thorax partially remaining

larva number few (< 50 %) hatched

embryo/egg with embryonic tissue, no cuticle, no fluorescence

adult lethality


adult lethality

motility decreased

eclosion not fulfilled (not completely eclosed)

adult lethality

larva lethality

larva cuticle duplicated

Larvae seem to have died right after hatching or just before molting.

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

larva number hatched none (< 10 %)


larva lethality


larva lethality early (L5/6)

vertex triplet irregular

gena triplet irregular

procephalic head mostly not present

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

abdomen musculature partially orientation irregular

abdomen musculature fragmented

abdomen musculature partially not present

larva number few (< 50 %) hatched


pupal molt delayed (still prepupa)

pupation delayed (still prepupa)

larva number hatched none (< 10 %)

adult nutritional state bad (cachectic)

adult lethality


larva lethality early (L5/6)

larva number hatched many (ca. 70-100 %)

adult lethality


larva lethality early (L5/6)

larva number few (< 50 %) hatched

abdomen partially not present

head & thorax partially not present

cuticle remnants strongly fragmented (cuticle crumbs)

larval musculature irregular

adult lethality


fertility after larval injection decreased (no living offspring)

vitellarium not present

vitellogenic egg chamber not present

previtellogenic egg chamber not present

mature egg not present

tropharium shape irregular

tropharium rounded

larva lethality early (L5/6)

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

egg chamber not present

egg production: number <50% of expected

adult lethality

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (died as pupa)


pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

larva lethality

adult lethality

larva number hatched none (< 10 %)

adult lethality


ovary without detectable defects

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

larva lethality

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)


pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

pupation delayed (still prepupa)

larva lethality early (L5/6)

adult lethality


larva lethality early (L5/6)

adult lethality

eclosion not fulfilled (with pupal and adult features)


ovary without detectable defects

egg production: number <50% of expected

adult lethality immediately after eclosion

larva number hatched none (< 10 %)

previtellogenic egg chamber shape irregular

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

larva lethality early (L5/6)


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

larva number hatched none (< 10 %)

egg chamber number decreased

ovary size decreased

egg production: number no/almost no eggs

adult lethality

adult lethality


elytra duplicated

wings blistered

eclosion not fulfilled (died as pupa)

larva lethality early (L5/6)

adult lethality

larva number hatched none (< 10 %)

adult lethality


larva number hatched none (< 10 %)

vitellogenic egg chamber deposition delayed

vitellogenic egg chamber number increased


larva lethality early (L5/6)

egg production: number <50% of expected


pupa lethality during eclosion

oblique dorsal thoracic muscles thinner

dorsal thoracic muscles number increased

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number few (< 50 %) hatched

egg chamber mostly not present

embryo/egg not developed/not fertilized

egg production: number <50% of expected


pupal molt delayed (still prepupa)

larva lethality early (L5/6)

larva number hatched none (< 10 %)

vitellarium size decreased

egg chamber mostly not present


ovary without detectable defects

egg production: number <50% of expected

larva lethality

procephalic head partially everted (inside-out)

labrum not present

legs partially remaining

cuticle remnants without visible segmentation, differentiation or axis

procephalic head not present

larva everted (inside-out)

larva number hatched none (< 10 %)

egg production: number <50% of expected


larva lethality early (L5/6)


larva lethality early (L5/6)

adult lethality


adult lethality

adult lethality

adult lethality


larva lethality late (L7)

larva lethality late (L7)

pupation delayed (still larva)

larva lethality early (L5/6)

pupal molt delayed (still larva)

pupal molt delayed (still pupa)

pupal molt not fulfilled (died as pupa)

wings shortened

pupal molt not fulfilled (died as pupa)

larva number hatched none (< 10 %)

egg chamber number decreased

adult lethality

adult lethality

egg chamber partially not present

adult lethality

adult lethality

adult lethality


pupal molt delayed (still larva)

larva lethality early (L5/6)

affected eggs/embryos/larvae

larval musculature irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


larva lethality early (L5/6)

affected eggs/embryos/larvae

adult lethality


pupal molt delayed (still prepupa)

larva lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


larva lethality early (L5/6)

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


larva lethality early (L5/6)

posterior terminus not present

abdomen shortened

labium not present

head & thorax partially not present

cuticle remnants with some identifiable segments


adult lethality

elytra not closed

thorax blistered

hindwing orientation irregular

adult lethality

larva number hatched none (< 10 %)

embryo/egg not developed/not fertilized


pupal molt not fulfilled (died with pupal and larval features)

larva lethality early (L5/6)


larva lethality

embryo/egg with embryonic tissue, no cuticle, no fluorescence

embryo/egg not developed/not fertilized

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

adult lethality

larva number hatched none (< 10 %)

eclosion not fulfilled (with pupal and adult features)


larva lethality early (L5/6)

larva number hatched none (< 10 %)

ovary size decreased

egg chamber not present


larva lethality early (L5/6)

eclosion not fulfilled (died as pupa)

larva lethality

head mostly remaining

larva number hatched none (< 10 %)

embryo/egg not developed/not fertilized

adult lethality

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

elytra shortened

pupation delayed (still larva)

eclosion not fulfilled (died as pupa)

larva number few (< 50 %) hatched

embryo/egg not developed/not fertilized

egg chamber number decreased

egg production: number <50% of expected

adult lethality


larva lethality early (L5/6)

pupa eclosion not fulfilled (died as pupa)


larva lethality

adult lethality


larva lethality early (L5/6)

affected eggs/embryos/larvae

larval musculature irregular


pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still prepupa)

larva lethality

larva number hatched none (< 10 %)

adult lethality

adult nutritional state bad (cachectic)

adult lethality


larva lethality early (L5/6)


larva lethality early (L5/6)

adult lethality

larva lethality

adult lethality

adult lethality

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


larva lethality early (L5/6)

mature egg not present

egg production: number no/almost no eggs

adult lethality

adult lethality

adult lethality immediately after eclosion


ovary without detectable defects

egg production: number <50% of expected

larval musculature irregular

larval musculature fluorescence decreased

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

egg production: number <50% of expected

adult lethality


ovary without detectable defects

egg production: number <50% of expected

larva number hatched many (ca. 70-100 %)

larval musculature irregular

egg production: number <50% of expected

adult lethality


larva lethality early (L5/6)

larval musculature fluorescence not present

abdomen musculature partially not present

adult lethality


adult lethality

larva lethality early (L5/6)

adult lethality


larva lethality early (L5/6)

adult lethality


adult lethality

wings not closed

eclosion not fulfilled (not completely eclosed)

abdomen anteroposterior-axis increased

oblique dorsal thoracic muscles fragmented

adult nutritional state bad (cachectic)


ovary without detectable defects

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


pupation delayed (still prepupa)

larva lethality early (L5/6)

larva number hatched none (< 10 %)

embryo/egg not developed/not fertilized

adult lethality

adult lethality


fertility after larval injection decreased (no living offspring)

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality early (L5/6)

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)


ovary without detectable defects

egg production: number <50% of expected

larva lethality early (L5/6)

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

egg chamber shape irregular

egg chamber size irregular

mature egg deposition 5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality


pupal molt not fulfilled (died as L7 or prepupa)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

eclosion not fulfilled (died as pupa)

larva lethality early (L5/6)

larva number hatched none (< 10 %)

egg chamber mostly not present


eclosion not fulfilled (with pupal and adult features)

wings shortened

larva lethality

larva number hatched none (< 10 %)

vitellogenic egg chamber shape irregular

egg chamber number decreased


pupal molt not fulfilled (died as prepupa)

pupation delayed (still prepupa)

larva lethality early (L5/6)

larva number hatched none (< 10 %)

egg chamber number decreased

vitellarium shortened

egg production: number <50% of expected

adult lethality


pupal molt delayed (still larva)

larva lethality early (L5/6)

adult lethality


larva lethality early (L5/6)

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

embryo/egg with embryonic tissue, no cuticle, no fluorescence


hindwing distal pigmentation/tanning not present

pupal molt not fulfilled (died as pupa)

affected eggs/embryos/larvae

larval musculature degenerated

larva number hatched none (< 10 %)

adult lethality


adult lethality

not fulfilled (died as pupa)


adult lethality immediately after eclosion

larva lethality

adult lethality

larva potentially partially fragmented

larva partially everted (inside-out)

larva everted (inside-out)

affected eggs/embryos/larvae


ovary without detectable defects

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

larva lethality

affected eggs/embryos/larvae

affected eggs/embryos/larvae

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality

adult lethality


pupal molt delayed (still prepupa)

ovariole mostly not present

ovary size decreased

egg production: number no/almost no eggs

adult lethality


pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number hatched many (ca. 70-100 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

pupa eclosion not fulfilled (died as pupa)


pupation delayed (still larva)

larva lethality early (L5/6)

adult lethality


adult lethality

labrum size decreased

mandible potentially transformed

mandible not present

maxilla number increased

affected eggs/embryos/larvae

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality


larva lethality early (L5/6)


eclosion not fulfilled (not completely eclosed)

wings blistered

larva lethality

larva number hatched none (< 10 %)

adult lethality


pupa cuticle decreased

head & thorax & abdomen potentially necrotic

pupa cuticle colour spotted

larva number hatched none (< 10 %)

adult lethality

adult lethality


wings shortened

legs position irregular

antenna position irregular

head position irregular

pupation not fulfilled (died as prepupa)

adult lethality

adult lethality


pupal molt not fulfilled (with pupal and adult features)

wings shortened

pupa shortened

pupation delayed (still larva)

larva lethality early (L5/6)

larva number hatched none (< 10 %)

vitellogenic egg chamber late number decreased

egg production: number <50% of expected

adult lethality


adult lethality

larva lethality early (L5/6)

affected eggs/embryos/larvae

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

egg chamber number decreased

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality early (L5/6)

larva number few (< 50 %) hatched


adult lethality

larva lethality early (L5/6)

thorax & abdomen dorsal not closed

head & thorax & abdomen segmentation/division irregular

abdomen segment number decreased

segment number decreased

adult lethality

adult lethality


larva lethality early (L5/6)

affected eggs/embryos/larvae

with embryonic tissue, no cuticle, no fluorescence

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


not fulfilled (died as prepupa)

pupal molt not fulfilled (died as prepupa)

larva lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


larva lethality early (L5/6)

adult lethality

adult lethality


wings potentially shortened

abdominal segment 8  differentiation irregular

wings orientation irregular

head orientation irregular

adult lethality


adult lethality

pupal molt not fulfilled (died as L7 or prepupa)

larva lethality


larva lethality


pupal molt delayed (still larva)

larva lethality early (L5/6)

adult lethality


tropharium size decreased

tropharium shape irregular

vitellogenic egg chamber early number increased

egg production: number no/almost no eggs

vitellogenic egg chamber late decreased

adult lethality immediately after eclosion

ovary without detectable defects

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality


adult lethality

larva lethality early (L5/6)

larva number hatched many (ca. 70-100 %)


adult lethality

wings orientation irregular

adult lethality

oblique dorsal thoracic muscles partially not present

oblique dorsal thoracic muscles anterior shortened


ovary without detectable defects

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality

pupa eclosion not fulfilled (died as pupa)


ovariole decreased

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva lethality early (L5/6)

larva number few (< 50 %) hatched

adult lethality


egg chamber fused

adult lethality

vitellogenic egg chamber number decreased

previtellogenic egg chamber not present

adult lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


larva lethality early (L5/6)

affected eggs/embryos/larvae

affected eggs/embryos/larvae

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


larva lethality early (L5/6)

affected eggs/embryos/larvae

ovary without detectable defects

adult lethality

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


larva lethality

lateral oviduct number degenerated

egg chamber number decreased

adult lethality


adult lethality

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality early (L5/6)


head anteroposterior-axis shortened

head appendages orientation irregular

pupal molt delayed (still larva)

larva lethality early (L5/6)

egg chamber complete not present

adult lethality


pupal molt not fulfilled (with pupal and adult features)

pupal molt not fulfilled (died as pupa)

pupal molt not fulfilled (died as pupa)

wings orientation irregular

wings partially blistered

pupal molt delayed (still larva)

adult lethality


adult lethality

ovary without detectable defects

egg production: number <50% of expected

larva lethality

adult lethality

adult lethality


pupal molt delayed (still larva)

larva lethality early (L5/6)

larva number hatched none (< 10 %)

adult lethality


adult lethality

elytra not closed

hindwing irregular

larva lethality early (L5/6)

affected eggs/embryos/larvae

head & thorax segmentation/division irregular

head structure irregular

thorax & abdomen bristle pattern irregular

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality

adult lethality

larva partially everted (inside-out)

larva everted (inside-out)

larval musculature random not present

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

hindwing irregular

elytra partially blistered

adult lethality

not fulfilled (with pupal and adult features)

wings slightly blistered

egg chamber size decreased

egg chamber shape irregular

egg chamber number decreased

egg production: number no/almost no eggs

adult lethality


adult lethality immediately after eclosion

larva number hatched many (ca. 70-100 %)

adult lethality


affected eggs/embryos/larvae

head & thorax & abdomen segment number decreased

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched


mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

ovary without detectable defects

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

affected eggs/embryos/larvae

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


adult lethality

ovary without detectable defects

egg production: number <50% of expected

larva number hatched none (< 10 %)

adult lethality

pupa eclosion not fulfilled (died as pupa)


larva lethality early (L5/6)

affected eggs/embryos/larvae

adult lethality


wings blistered

antenna orientation irregular

affected eggs/embryos/larvae

larva appendages shape irregular

larva appendages mostly size decreased

larva cuticle dorsal not closed

larva segment shape irregular

larva appendages sometimes number decreased

abdomen bristle ectopically increased

ventral ectopic stripes in AP direction.

antenna potentially transformed

larva segment number decreased

vitellogenic egg chamber late degenerated

egg chamber size decreased

egg chamber shape rounded

adult lethality

larva number hatched none (< 10 %)

adult lethality

egg production: number <50% of expected

adult lethality


larva lethality

affected eggs/embryos/larvae

affected eggs/embryos/larvae

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

larva number hatched none (< 10 %)


pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

vitellogenic egg chamber early potentially degenerated

vitellogenic egg chamber late not present

ovary size decreased

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality early (L5/6)

adult lethality


affected eggs/embryos/larvae

egg chamber degenerated

egg chamber partially number decreased

adult lethality

larva number hatched none (< 10 %)

adult lethality

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


pupal molt delayed (still larva)

larva lethality early (L5/6)

adult lethality

adult lethality


larva lethality early (L5/6)

affected eggs/embryos/larvae

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


pupal molt not fulfilled (with pupal and adult features)

adult lethality immediately after eclosion

larva lethality early (L5/6)

egg chamber mostly not present

adult lethality

egg production: number no/almost no eggs


larva lethality early (L5/6)

larva number hatched many (ca. 70-100 %)

adult lethality


not fulfilled (died as L7 or prepupa)

legs shape irregular

legs position irregular

antenna shape rounded

antenna shape shortened

female genital lobes rounded

wings decreased

gnathal appendages position irregular

pupal molt delayed (still prepupa)

larva lethality

egg chamber number decreased

adult lethality


ovary without detectable defects

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality


adult lethality

larva lethality early (L5/6)

larva number few (< 50 %) hatched

adult lethality

adult lethality


adult lethality


larva lethality early (L5/6)

adult lethality

adult lethality


ovary without detectable defects

egg production: number <50% of expected

larva lethality early (L5/6)

adult lethality


adult lethality

larva lethality

adult lethality

adult lethality


wings not closed

head & thorax & abdomen orientation irregular

larval musculature spotted

larval musculature partially not present

larva number hatched none (< 10 %)

adult lethality


wings not closed

larval musculature spotted

larva number hatched none (< 10 %)

adult lethality

larva number hatched none (< 10 %)


larva lethality

adult lethality


larva lethality early (L5/6)

adult lethality

pupa eclosion not fulfilled (died as pupa)


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

larva number hatched none (< 10 %)

adult lethality


delayed (still larva)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

pupal molt not fulfilled (died as L7 or prepupa)

adult lethality

adult lethality


ovary without detectable defects

adult lethality

affected eggs/embryos/larvae

affected eggs/embryos/larvae

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

vitellogenic egg chamber late size decreased

vitellogenic egg chamber late number increased

egg chamber potentially segmentation/division not present

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality


adult lethality

larva lethality early (L5/6)

adult lethality


larva lethality early (L5/6)

egg shell potentially irregular

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality

posterior terminus sometimes decreased

abdomen segment number decreased

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide


adult lethality

larva lethality early (L5/6)

adult lethality

adult lethality


wings not closed

pupal molt delayed (still larva)

larva lethality early (L5/6)

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality


adult lethality

hindwing potentially folding irregular

wings slightly orientation irregular

female genital lobes spotted

potentially feces at the developing proctodeum?

oblique dorsal thoracic muscles slightly broader

female genital lobes shortened

oblique dorsal thoracic muscles slightly shortened

hindwing slightly position irregular

abdomen segment partially not present

head & thorax & abdomen ventral increased

abdomen dorsal not closed

labium not fused

larval musculature fluorescence decreased

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

pupal molt not fulfilled (died as prepupa)

larva lethality

yolk potentially degenerated

yolk irregular

egg production: number no/almost no eggs

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

yolk potentially degenerated

yolk irregular

adult lethality


elytra distal not closed

wings not closed

larva lethality

larva number hatched many (ca. 70-100 %)

adult lethality

adult lethality

pupa eclosion not fulfilled (died as pupa)


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

larva number hatched none (< 10 %)

adult lethality

egg production: number <50% of expected

adult lethality


elytra duplicated

prothorax potentially transformed

prothorax size decreased

larva lethality

affected eggs/embryos/larvae

larval musculature pattern irregular

larva number hatched none (< 10 %)

adult lethality


adult lethality

affected eggs/embryos/larvae

head bristle partially not present

gena triplet gena triplet posterior mostly not present

larval musculature partially not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


larva lethality

head partially everted (inside-out)


eclosion delayed (still pupa)

eclosion delayed (still pupa)

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupation delayed (still larva)

larva number hatched none (< 10 %)

adult lethality

adult lethality


abdominal odoriferous glands size increased

vitellarium not present

tropharium not present

ovariole size decreased

tropharium size decreased

vitellarium not present


ovary without detectable defects

aimals potentially too young

egg production: number <50% of expected

affected eggs/embryos/larvae

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


abdominal odoriferous glands structure irregular

abdominal odoriferous glands shape irregular

abdominal odoriferous glands colour not present

pupal molt delayed (still larva)

eclosion not fulfilled (with pupal and adult features)


egg chamber potentially not present

vitellarium size decreased

egg chamber potentially fused

yolk ectopically deposition

tropharium size decreased

ovary without detectable defects

egg production: number <50% of expected


elytra shape irregular

elytra cuticle structure decreased

eclosion delayed (still pupa)

adult lethality

pupal molt delayed (still larva)

larva lethality

larva number few (< 50 %) hatched

adult lethality

adult lethality


pupa lethality during eclosion

female genital lobes potentially duplicated

oblique dorsal thoracic muscles partially shortened

wings shortened

antenna orientation irregular

pygopodium potentially size decreased

urogomphi potentially transformed

urogomphi not present

abdomen segment number # of remaining segments  increased

posterior terminus irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality


larva lethality

adult lethality

adult lethality

previtellogenic egg chamber position irregular

tropharium structure irregular

vitellogenic egg chamber late number decreased

mature egg not present

larva number hatched none (< 10 %)

adult lethality

adult lethality immediately after eclosion


larva lethality

adult lethality


pupal molt not fulfilled (died as prepupa)

affected eggs/embryos/larvae

larval musculature fluorescence decreased

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

pupa eclosion not fulfilled (died as pupa)


larva lethality

affected eggs/embryos/larvae

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg condensed embryonic tissue, no cuticle, cns fluorescence

abdomen musculature partially not present

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched


adult lethality

larva lethality

gena bristle number increased

labrum not present

labrum size decreased

larval musculature differentiation incomplete

larva number few (< 50 %) hatched

adult lethality


ovary without detectable defects

egg production: number <50% of expected

pupal molt delayed (still larva)

affected eggs/embryos/larvae

cuticle remnants with some identifiable segments

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

eclosion not fulfilled (with pupal and adult features)


pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

adult nutritional state bad (cachectic)

egg production: number <50% of expected

adult lethality


larva lethality

larva number hatched many (ca. 70-100 %)

adult lethality


pupa lethality as pupa

elytra necrotic

elytra blistered

dorsal thoracic muscles potentially differentiation irregular

wings shape irregular

antenna orientation irregular

eclosion not fulfilled (died as pupa)

egg chamber partially fused

egg production: number no/almost no eggs

egg chamber number decreased

larva number hatched none (< 10 %)

adult lethality


pupal molt delayed (still larva)

larva lethality late (L7)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

pupation delayed (still larva)

larva lethality late (L7)

adult nutritional state bad (cachectic)

adult lethality


eclosion delayed (still pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

adult lethality


eclosion not fulfilled (died as pupa)

wings not closed

wings shortened

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

vitellogenic egg chamber potentially differentiation aborted

mature egg not present

egg production: number <50% of expected

egg production: number <50% of expected


abdomen gin-traps partially orientation irregular

larva lethality

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

embryo/egg not developed/not fertilized

adult lethality


abdomen gin-trap setae number decreased

tropharium sometimes degenerated

mature egg not present

vitellogenic egg chamber differentiation aborted


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult nutritional state bad (cachectic)

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality late (L7)

mesonotum central colour darker

pupal molt delayed (still larva)

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

vitellogenic egg chamber mostly degenerated

egg production: number <50% of expected

adult lethality


pupal molt not fulfilled (died as prepupa)

larva number hatched none (< 10 %)

vitellogenic egg chamber number decreased

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected


eclosion not fulfilled (not completely eclosed)

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

pupa eclosion not fulfilled (died as pupa)


ovary without detectable defects

egg production: number <50% of expected

elytra partially blistered

wings not closed

adult lethality immediately after eclosion

larva lethality early (L5/6)

adult lethality

eclosion not fulfilled (with pupal and adult features)

affected eggs/embryos/larvae

affected eggs/embryos/larvae

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

larval musculature differentiation incomplete

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number few (< 50 %) hatched


pupal molt not fulfilled (died as pupa)

pupal molt not fulfilled (died as pupa)

larva lethality early (L5/6)

larva number hatched none (< 10 %)

egg production: number <50% of expected

Ovaries look wt

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality

pupal molt delayed (still pupa)

wings decreased

eclosion delayed (still pupa)

pupal molt delayed (still larva)

wings complete not present

pupal molt delayed (still larva)

larva lethality early (L5/6)

larva number few (< 50 %) hatched

egg chamber number decreased

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


wings not closed

antenna shortened

adult lethality

not fulfilled (died as pupa)

antenna orientation irregular

wings shortened

antenna shortened

pupal molt delayed (still larva)

larva lethality early (L5/6)

affected eggs/embryos/larvae

head & thorax & abdomen bristle partially not present

dorsal ridge row irregular

head & thorax & abdomen bristle pattern irregular

larval musculature pattern irregular

abdomen musculature specific subset dorsal broader

Abdominal muscles from anterior midline to posterior dorsal muscle-bundle are clearly broader than in wt. This effect was visible in weaker phenotypes.

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

ovary without detectable defects

larva number few (< 50 %) hatched

egg production: number <50% of expected


larva lethality early (L5/6)

mature egg deposition 3 blocked number 1st lateral oviduct:

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched many (ca. 70-100 %)

egg production: number <50% of expected

egg production: number <50% of expected


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality late (L7)

adult lethality

egg production: number no/almost no eggs

adult lethality


adult lethality

elytra not closed

wings shortened

wings shortened

larva lethality early (L5/6)

affected eggs/embryos/larvae

larva dorsal not closed

legs broader

larva appendages shape irregular

larval musculature irregular

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality

adult lethality


larva lethality early (L5/6)

head capsule size decreased

larval musculature potentially degenerated

larval musculature fluorescence decreased

egg production: number <50% of expected

larval musculature pattern irregular

larva number few (< 50 %) hatched

ovary without detectable defects

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

head partially not present

head & thorax & abdomen partially not present

cuticle remnants without visible segmentation, differentiation or axis

tropharium partially degenerated

vitellogenic egg chamber late degenerated

previtellogenic egg chamber number decreased

adult lethality

eclosion not fulfilled (with pupal and adult features)


pupal molt not fulfilled (died as prepupa)

wings potentially shortened

wings not closed

antenna orientation irregular

pupal molt delayed (still prepupa)

larva lethality early (L5/6)

pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

larva lethality

labium position irregular

vertex triplet vertex triplet ventral not present

gena triplet seta partially not present

mature egg deposition 2 >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

abdominal segment 1 cuticle ectopically with protrusion

legs pretarsus slightly shortened

dorsal ridge row mostly not present

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larval musculature pattern irregular

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality

egg production: number <50% of expected

abdomen segment size decreased

abdomen bristle potentially pattern irregular

head appendages random not present

head appendages shape irregular

larva bristle potentially pattern irregular

abdomen segment number decreased

thorax partially not present

head capsule size decreased

larval musculature fluorescence decreased

larval musculature irregular

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected


adult lethality

elytra surface rough

antenna shape bent

antenna slightly orientation irregular

affected eggs/embryos/larvae

larva number hatched many (ca. 70-100 %)

adult lethality

pupa eclosion not fulfilled (died as pupa)


elytra surface irregular

elytra shape irregular

elytra slightly shortened

thorax & abdomen partially not present

abdomen dorsal not closed

with embryonic tissue, no cuticle, but muscle fluorescence

larval musculature differentiation incomplete

embryo/egg not developed/not fertilized

adult lethality


larva lethality early (L5/6)

adult lethality


larva lethality late (L7)

larva lethality early (L5/6)

adult lethality

egg chamber sometimes segmentation/division not present

egg chamber number decreased

tropharium partially degenerated

adult lethality

egg production: number no/almost no eggs

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality late (L7)

larva lethality early (L5/6)

adult lethality

adult nutritional state bad (cachectic)

adult lethality


pupal molt not fulfilled (died as pupa)

affected eggs/embryos/larvae

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched

adult lethality

adult lethality


vitellogenic egg chamber increased

adult lethality

mature egg not present

larva lethality early (L5/6)

affected eggs/embryos/larvae

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)


pupal molt not fulfilled (died as pupa)

affected eggs/embryos/larvae

cuticle remnants with unidentifyable segments

embryo/egg with embryonic tissue, no cuticle, no fluorescence

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larval musculature pattern irregular

embryo/egg not developed/not fertilized

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected