larva number few (< 50 %) hatched

adult lethality


larva partially partially not closed

abdomen segment number decreased

head & thorax segment random not present

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

eclosion not fulfilled (with pupal and adult features)


larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality


affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larval musculature irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (died as pupa)


adult lethality

abdomen segment number decreased

abdomen segment partially fused

abdomen irregular

larva partially dorsal not closed

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae


abdomen segment number decreased

abdomen constricted

head remaining

abdomen irregular

head & thorax & abdomen segment random not present

affected eggs/embryos/larvae

App. 30%.

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

adult lethality


adult lethality

adult lethality


adult lethality

legs slightly swollen

legs number irregular

abdomen segment number decreased

legs shape irregular

affected eggs/embryos/larvae

larval musculature irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected


larva everted (inside-out)

larva partially dorsal not closed

affected eggs/embryos/larvae

vitellogenic egg chamber late not present

Females potentially too young.

adult lethality

larva number few (< 50 %) hatched

egg production: number <50% of expected


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


abdomen musculature random partially irregular

Hatched larvae stayed in a bend position (app. 30%).

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


labium potentially transformed

Diistal part of the labium potentially transformed to leg.

legs number increased

maxilla potentially transformed

Distal part of the maxialla potentially transformed to leg.

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

larva number few (< 50 %) hatched


legs potentially transformed

Coxa seems unaffected. Trochanter, femur, tibiatarsus, pretarsus mostly not present, potentially transformed to antenna.

legs pretarsus not present

legs tibiotarsus decreased

legs shape irregular

legs femur decreased

prothoracic leg shortened

Complete leg reduced to stumps.

prothorax decreased

affected eggs/embryos/larvae

larval musculature dorsal partially not fused

thorax musculature irregular

thorax musculature dorsal partially not present

thorax musculature partially not present

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality

adult lethality

adult lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality


head not present

head appendages random not present

Maxilla mostly present.

abdomen segment partially fused

thorax partially not present

head capsule not present

larva partially not closed

affected eggs/embryos/larvae

larval musculature irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality


ovary without detectable defects

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality


egg production: number no/almost no eggs

vitellogenic egg chamber late mostly not present

Females potentially too young.


affected eggs/embryos/larvae


abdomen segment number decreased

head & thorax segment random not present

cuticle remnants with some identifiable segments

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

eclosion not fulfilled (with pupal and adult features)


head & thorax partially not present

Head and prothorax not present, potentially due to defects concerned dorsal closure.

larva partially dorsal not closed

head capsule decreased

head appendages random not present

affected eggs/embryos/larvae

larval musculature irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

adult lethality


abdomen segment number decreased

thorax segment partially not present

larva partially dorsal not closed

affected eggs/embryos/larvae

abdomen musculature random irregular

larval musculature irregular

affected eggs/embryos/larvae

larva number hatched many (ca. 70-100 %)


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


ovary without detectable defects

egg production: number <50% of expected


pygopodium decreased

urogomphi decreased

posterior terminus irregular

larva shape irregular

Larvae seem not to be streched and stay in a compaqued position.

affected eggs/embryos/larvae

larval musculature fluorescence decreased

larval musculature irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


head capsule decreased

labrum potentially position irregular

abdomen segment number decreased

antenna not present

head & thorax segment partially not present

affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

abdomen musculature random irregular

App. 35% are affected.

larval musculature irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

adult lethality

adult lethality

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


abdomen number decreased

affected eggs/embryos/larvae

larval musculature fluorescence irregular

larval musculature irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


abdomen musculature partially irregular

larval musculature irregular

affected eggs/embryos/larvae

adult lethality


mature egg deposition 4 5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:


adult nutritional state bad (cachectic)


head appendages orientation irregular

larva appendages partially shape irregular

larva partially dorsal not closed

head capsule decreased

larva everted (inside-out)

affected eggs/embryos/larvae

larval musculature irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

egg chamber shape irregular

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

embryonic tissue strongly fragmented (crumbs)

egg production: number no/almost no eggs

adult lethality

adult lethality


adult lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

adult lethality


larval musculature irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


head bristle irregular

The irregular head bristle pattern is potentially due to the decreased head capsule (secondary defect).

head capsule irregular

head capsule decreased

larva partially dorsal not closed

affected eggs/embryos/larvae

larval musculature irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected


adult lethality

ovary without detectable defects

egg production: number <50% of expected

affected eggs/embryos/larvae

abdominal odoriferous glands size decreased

larval musculature pattern irregular

affected eggs/embryos/larvae

previtellogenic egg chamber amount increased

mature egg not present

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


adult lethality

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

eclosion not fulfilled (with pupal and adult features)


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number

egg production: number <50% of expected

adult lethality


larva number hatched many (ca. 70-100 %)


ovary without detectable defects

Females potentially too young.

larva number hatched none (< 10 %)


adult lethality

eclosion not fulfilled (died as pupa)


larva partially everted (inside-out)

larva everted (inside-out)

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

adult lethality

adult lethality


adult lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


larva segment partially fused

larva partially potentially not closed

affected eggs/embryos/larvae

larval musculature dorsal partially irregular

larval musculature irregular

affected eggs/embryos/larvae

adult lethality

eclosion not fulfilled (died as pupa)


adult nutritional state potentially bad (cachectic)


adult lethality


antenna not present

head capsule irregular

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


larva everted (inside-out)

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

More than 80% hatched larvae on slide, no phenotype scorable. Due to the muscle phenotype, larvae potentially not able to move.

larval musculature fluorescence not present

larval musculature potentially not present

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)


head irregular

head segment random not present

affected eggs/embryos/larvae

larval musculature irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


vitellogenic egg chamber early not present

vitellogenic egg chamber late not present

vitellarium decreased

adult lethality


adult lethality

adult lethality


abdomen segment number decreased

abdomen irregular

larva partially dorsal not closed

affected eggs/embryos/larvae

larval musculature irregular

Hatched larvae show specifically abdominal muscle defects.

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


abdomen segment partially fused

abdomen segment number decreased

abdomen irregular

affected eggs/embryos/larvae

adult lethality


adult lethality

antenna shape irregular

affected eggs/embryos/larvae

larval musculature fluorescence decreased

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

egg production: number no/almost no eggs

adult lethality


adult lethality

No fluorescence potentially due to dead/ dying.

larval musculature potentially degenerated

larval musculature poetentially degenerated and irregular due to dead/ dying.

larval musculature irregular

affected eggs/embryos/larvae


larval musculature irregular

affected eggs/embryos/larvae

eclosion not fulfilled (died as pupa)


larva everted (inside-out)

affected eggs/embryos/larvae

larval musculature irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


larva partially dorsal not closed

affected eggs/embryos/larvae

adult lethality

ovary without detectable defects

mature egg deposition 2 5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected


adult lethality

ovary without detectable defects

adult lethality

adult lethality


abdomen segment number decreased

larva circumference irregular

affected eggs/embryos/larvae

larval musculature irregular

Potentially due to death.

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult nutritional state bad (cachectic)

adult lethality

labrum size decreased

mandible potentially transformed

mandible not present

maxilla number increased

affected eggs/embryos/larvae

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected


adult lethality


adult lethality

ovary without detectable defects


egg production: number <50% of expected


ovary without detectable defects

mature egg deposition 2 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

eclosion not fulfilled (with pupal and adult features)


abdomen segment partially fused

abdomen bristle irregular

abdomen irregular

gena triplet orientation irregular

affected eggs/embryos/larvae

larval musculature irregular

App. 35% are affected.

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

adult lethality


abdomen segment partially fused

head capsule decreased

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched


larva number hatched none (< 10 %)


affected eggs/embryos/larvae

adult lethality


tropharium potentially structure irregular

vitellogenic egg chamber late number decreased

adult lethality

vitellogenic egg chamber early number decreased


ovary without detectable defects

adult lethality

adult nutritional state bad (cachectic)

adult lethality


abdomen partially everted (inside-out)

abdomen segment number decreased

head & thorax & abdomen partially not present

cuticle remnants with unidentifyable segments

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larval musculature irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number few (< 50 %) hatched

adult lethality


larva number few (< 50 %) hatched

adult lethality

adult lethality


abdomen segment number decreased

larva partially dorsal not closed

affected eggs/embryos/larvae

adult lethality


adult lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

vitellogenic egg chamber late mostly degenerated

vitellogenic egg chamber early not present

egg chamber mostly not present

adult lethality

adult lethality

egg production: number no/almost no eggs


adult lethality

posterior terminus shape irregular

abdomen partially shape irregular

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected


adult lethality


larva everted (inside-out)

larva partially everted (inside-out)

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


vitellogenic egg chamber late potentially degenerated

egg chamber potentially number decreased

adult lethality

adult lethality


dorsal ridge row irregular

labrum not present

head bristle partially not present

head bristle pattern irregular

head capsule size decreased

affected eggs/embryos/larvae

abdomen musculature specific subset dorsal partially not present

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality


larva number hatched many (ca. 70-100 %)


adult lethality

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality


larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

adult lethality


affected eggs/embryos/larvae

embryo/egg

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality

adult lethality

adult lethality


mature egg deposition 4 5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected


embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

vitellogenic egg chamber late sometimes fused

vitellogenic egg chamber late mostly not present

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality

vitellogenic egg chamber early mostly not present

egg chamber number decreased


adult lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larval musculature fluorescence decreased

affected eggs/embryos/larvae

adult lethality


adult lethality

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

abdomen segment number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality


egg production: number no/almost no eggs

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality


eclosion not fulfilled (died as pupa)


adult lethality


ovary without detectable defects

adult lethality


antenna sometimes shape irregular

head appendages random not present

labrum shape irregular

labrum size decreased

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult lethality

adult lethality


head appendages random not present

head capsule size decreased

larva partially dorsal not closed

affected eggs/embryos/larvae

adult lethality

adult lethality


adult nutritional state

adult lethality


larva partially constricted

abdomen segment number decreased

thorax segment partially random not present

larva partially dorsal not closed

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

adult lethality


adult lethality

head appendages size decreased

abdomen segment number decreased

head appendages rounded

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


egg chamber mostly not present

egg chamber number decreased

lateral oviduct potentially structure irregular


adult lethality

adult lethality


abdomen segment number decreased

larva appendages sometimes shape irregular

posterior terminus sometimes lighter

legs podomer partially not present

head appendages sometimes position irregular

head & thorax segment random not present

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

eclosion not fulfilled (with pupal and adult features)


larva number hatched many (ca. 70-100 %)

adult lethality

adult lethality


vitellogenic egg chamber late degenerated

vitellogenic egg chamber late potentially fused

abdominal odoriferous glands content darker

egg chamber number decreased

adult lethality


head appendages random not present

head capsule decreased

larva everted (inside-out)

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched


abdomen segment number decreased

abdomen circumference irregular

larva partially dorsal not closed

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


abdomen segment number decreased

abdomen circumference irregular

larva partially dorsal not closed

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


eclosion not fulfilled (died as pupa)

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


head & thorax appendages partially shape irregular

head & thorax appendages partially position irregular

head & thorax & abdomen segment random not present

Strong phenotypes do not show any head or thoracic segments.

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

egg production: number no/almost no eggs

adult lethality


ovary without detectable defects

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality


larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

adult lethality


adult nutritional state

adult lethality


adult lethality

adult lethality


adult lethality

adult lethality

adult lethality

abdominal odoriferous glands partially content opaque

abdominal odoriferous glands content split


adult lethality

adult lethality

adult lethality


adult lethality

adult lethality

adult lethality

adult lethality


adult lethality

larva number few (< 50 %) hatched


adult lethality

egg production: number <50% of expected


adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality

adult lethality


previtellogenic egg chamber potentially not present

vitellogenic egg chamber late mostly not present

lateral oviduct structure irregular

egg chamber decreased

abdominal odoriferous glands content fragmented

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva number hatched many (ca. 70-100 %)


adult lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (died as pupa)


abdomen circumference irregular

abdomen segment number decreased

abdomen segment partially fused

abdomen irregular

abdomen seta pattern irregular

head & thorax segment bilateral complete not present

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


larva number few (< 50 %) hatched

larval musculature partially not present

larval musculature partially spotted

larval musculature partially degenerated

affected eggs/embryos/larvae


adult lethality

ovary without detectable defects

egg chamber late partially not present

egg chamber partially degenerated

adult lethality


adult lethality


vitellogenic egg chamber late decreased

adult lethality

adult lethality


larval musculature lateral potentially size decreased

larval musculature lateral distal irregular

affected eggs/embryos/larvae

adult lethality


embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult lethality

ovary without detectable defects

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality

adult lethality


adult lethality

adult lethality

ovary without detectable defects

egg production: number <50% of expected

adult lethality

adult lethality


adult lethality

egg chamber irregular

egg chamber potentially fused

vitellogenic egg chamber late potentially degenerated

egg production: number no/almost no eggs

adult lethality


adult nutritional state potentially bad (cachectic)

egg chamber decreased

adult lethality


vertex triplet vertex triplet ventral not present

legs femur not present

legs trochanter potentially not present

legs shape irregular

legs size decreased

head appendages mostly size decreased

labium distal structure not present

maxilla distal structure not present

legs podomer mostly not present

Coxa, tibiotarsus (potentially) and pretarsus present.

head appendages mostly shape irregular

affected eggs/embryos/larvae


adult lethality

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

larval musculature not present

larval musculature degenerated

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality


adult lethality

head & thorax segment random not present

head & thorax & abdomen segment random not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva number hatched many (ca. 70-100 %)


adult lethality

adult lethality

cuticle remnants with unidentifyable segments

head appendages position irregular

head & thorax & abdomen segment shape irregular

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

adult lethality

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


abdomen irregular

abdomen segment number decreased

Potentially due to problems with dorsal closure.

larva partially dorsal not closed

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality


affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched

adult lethality


larva number few (< 50 %) hatched

adult lethality


head & thorax not present

head & thorax segment random partially not present

affected eggs/embryos/larvae

adult lethality


affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality

eclosion not fulfilled (died as pupa)


adult lethality

adult lethality

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult nutritional state

larva number few (< 50 %) hatched


abdominal segment 1 ectopically structure

Abdominal segment 1 shows an ectopically leg like structure.

abdominal segment 2 ectopically structure

Abdominal segment 2 shows an ectopically leg like structure.

mesothoracic leg potentially transformed

Mesothoracic leg potentially transformed to a palp like strukture.

mesothoracic leg shape irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


mesothoracic leg shape irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


mesothoracic leg shape irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


head capsule decreased

In few cases the head is missing completely.

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

affected eggs/embryos/larvae

ovary without detectable defects

adult lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

adult lethality


abdomen musculature lateral distal pattern irregular

abdomen musculature lateral potentially size decreased

affected eggs/embryos/larvae

ovary without detectable defects

adult nutritional state potentially bad (cachectic)

adult lethality

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (died as pupa)


head appendages random not present

head & thorax appendages partially shape irregular

affected eggs/embryos/larvae

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva elongated

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality

adult lethality


larva number hatched many (ca. 70-100 %)

adult lethality immediately after eclosion


abdomen irregular

abdomen segment number decreased

abdomen circumference irregular

abdomen segment potentially partially fused

larva partially dorsal not fused

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality

adult lethality


adult lethality

adult lethality

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


head appendages partially shape irregular

head capsule size decreased

head & thorax segment random not present

head appendages random not present

larva partially constricted

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult lethality


affected eggs/embryos/larvae


adult lethality


adult lethality

vitellogenic egg chamber late potentially fused

vitellogenic egg chamber late structure irregular

vitellogenic egg chamber late shape irregular

adult lethality

egg production: number no/almost no eggs

adult lethality


adult lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality

adult lethality


metathorax not present

cuticle remnants with some identifiable segments

abdomen segment number decreased

thorax shape irregular

thorax & abdomen segment partially not present

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, no fluorescence

All eggs show a slight fluorescence in the posterior part.

affected eggs/embryos/larvae

adult lethality


adult lethality

abdomen segment partially potentially fused

abdomen segment number decreased

abdomen irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

eclosion not fulfilled (with pupal and adult features)


antenna not present

head appendages partially not present

head capsule size decreased

labrum potentially position irregular

head appendages partially position irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality

adult lethality


adult lethality

affected eggs/embryos/larvae

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


larva number hatched many (ca. 70-100 %)

adult lethality


gena triplet gena triplet dorsal not present

vertex triplet vertex triplet posterior not present

abdomen seta random not present

head seta pattern irregular

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


egg production: number <50% of expected

adult lethality

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected


egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched

adult lethality

eclosion not fulfilled (died as pupa)


embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


thorax & abdomen segment potentially partially fused

legs pretarsus bent

gnathal appendages partially not present

head & thorax & abdomen partially not present

larval musculature pattern irregular


larva appendages partially shape irregular

adult lethality


abdomen segment partially fused

head & thorax partially not present

head and thorax are either partial missing or appear partial fused

prothorax not present

head appendages shape irregular

gut partially remaining

cuticle remnants with some identifiable segments

larva number few (< 50 %) hatched


abdomen segment potentially transformed

legs number increased

head mostly not present

cuticle remnants without visible segmentation but polarity

larval musculature irregular

larva number hatched none (< 10 %)


legs segment shortened

procephalic appendages mostly not present

sometimes one deformed antenna remaining

head & thorax partially not present

larval musculature irregular

larva number hatched none (< 10 %)

adult lethality immediately after eclosion

eclosion not fulfilled (with pupal and adult features)

affected eggs/embryos/larvae


adult lethality


legs segment potentially fused

legs segment shortened

cuticle remnants with unidentifyable segments

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number few (< 50 %) hatched

adult lethality


procephalic appendages sometimes remaining

abdomen partially remaining

larval musculature irregular

larva number few (< 50 %) hatched


larva number few (< 50 %) hatched

adult lethality


larva number hatched many (ca. 70-100 %)


legs segment sometimes potentially fused

gnathal appendages shape irregular

procephalic appendages not present

head & thorax & abdomen cuticle dorsal irregular

legs segment sometimes shortened

head capsule not present

cuticle remnants strongly fragmented (cuticle crumbs)

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larval musculature irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larva number hatched none (< 10 %)


larval musculature irregular

adult lethality


larval musculature irregular

larva number hatched none (< 10 %)

ovary without detectable defects

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


head bristle irregular

head appendages random not present

legs segment potentially fused

abdomen segment number decreased

legs segment shortened

head capsule partially not present

head & thorax & abdomen partially not present

larval musculature irregular

larva number hatched none (< 10 %)


thorax & abdomen partially present

larval musculature pattern irregular

adult lethality


head & thorax partially not present

legs shape irregular

cuticle remnants with some identifiable segments

embryo/egg without visible larval cuticle in cuticle preparation

larval musculature irregular

larva number hatched none (< 10 %)

adult lethality


larval musculature mostly shortened

larval musculature mostly not present

adult lethality


larva dorsal not closed

legs shape irregular

labium not present

larva everted (inside-out)

cuticle remnants cuticle remnants with some identifiable segments

larval musculature irregular

larval musculature partially not present

larva number few (< 50 %) hatched

adult lethality

eclosion not fulfilled (with pupal and adult features)


embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

pupa eclosion not fulfilled (died as pupa)


embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)


procephalic head not present

head not present

head & thorax partially not present

abdomen posterior partially remaining

larva number few (< 50 %) hatched

larval musculature pattern irregular


legs potentially fused

abdomen bristle segmentation/division irregular

thorax anterior not present

head posterior ventral not present

head & thorax partially not present

about half of the remnants appear inside out

larval musculature irregular

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched

adult lethality


adult lethality


larval musculature irregular

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


embryo/egg not developed/not fertilized

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number hatched none (< 10 %)

adult lethality


head & thorax partially not present

larva cuticle dorsal not closed

gnathal appendages random remaining

procephalic appendages random remaining

abdomen segment number decreased

legs podomer shortened

cuticle everted (inside-out)

cuticle remnants with unidentifyable segments

larva number hatched none (< 10 %)

larval musculature irregular


head & thorax partially not present

larva cuticle dorsal not closed

gnathal appendages random remaining

procephalic appendages random remaining

abdomen segment number decreased

legs podomer shortened

cuticle everted (inside-out)

larva number hatched none (< 10 %)

larval musculature irregular


affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)


eclosion not fulfilled (died as pupa)


larva appendages shortened

cuticle remnants without visible segmentation, differentiation or axis

embryo/egg not developed/not fertilized

adult lethality


procephalic head partially not present

procephalic appendages shape irregular


larva cuticle partially unilateral not present

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larval musculature irregular

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched

adult lethality

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


legs shortened

head & thorax & abdomen segment random not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality


legs shape irregular

head appendages random not present

larva appendages irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva segment random not present

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


abdomen complete not present

legs podomer shortened

cuticle remnants strongly fragmented (cuticle crumbs)

cuticle everted (inside-out)

gnathal appendages mostly remaining

cuticle remnants with some identifiable segments

abdomen segment number decreased

thorax partially not present

head appendages random not present

head & thorax & abdomen partially not present

procephalic appendages mostly remaining

embryo/egg without visible larval cuticle in cuticle preparation

larval musculature irregular

larva number few (< 50 %) hatched


adult lethality


larva number hatched none (< 10 %)


larval musculature irregular

adult lethality


labium not present

head size decreased

affected eggs/embryos/larvae

mandible not present

abdomen segment number decreased

prothorax not present

maxilla not present

larva number few (< 50 %) hatched

adult lethality


maxilla orientation irregular

labium orientation irregular

mesothorax not present

metathorax not present

legs size decreased

abdomen segment not present

legs shape irregular

head appendages shape irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

larval musculature pattern irregular

affected eggs/embryos/larvae


abdomen partially everted (inside-out)

larval musculature irregular

adult lethality


legs sometimes shape irregular

maxilla shape irregular

labium shape irregular

labium orientation irregular

legs sometimes number decreased

maxilla orientation irregular

head & thorax segment sometimes not present

affected eggs/embryos/larvae

adult lethality

eclosion not fulfilled (with pupal and adult features)


affected eggs/embryos/larvae

adult lethality


head appendages random not present

head appendages sometimes shape irregular

thorax & abdomen not present

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


larva appendages partially shape irregular

larva segment median partially decreased

affected eggs/embryos/larvae

adult lethality

larva number hatched many (ca. 70-100 %)


embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


thorax partially not present

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


prothorax sometimes not present

head & thorax segment partially not present

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

eclosion not fulfilled (died as pupa)


larva segment posterior random number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


previtellogenic egg chamber partially shape irregular

egg production: number <50% of expected

egg production: number <50% of expected


eclosion not fulfilled (died as pupa)


adult lethality


adult lethality

abdomen segment potentially fused

thorax segment potentially fused

head size decreased

abdomen segment number decreased

thorax segment number decreased

head appendages partially not present

larva segment number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched


adult lethality

adult lethality


head shape irregular

abdomen segment number decreased

legs shape irregular

head appendages partially not present

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

adult lethality

previtellogenic egg chamber anterior not present

mature egg not present

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


legs shape irregular

abdomen segment number decreased

thorax segment number decreased

head appendages partially not present

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


abdomen segment number decreased

thorax segment number decreased

thorax & abdomen not present

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

affected eggs/embryos/larvae

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality

adult lethality


adult lethality

pupa eclosion not fulfilled (died as pupa)


mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

mature egg deposition 5 blocked number 1st lateral oviduct:

ovary without detectable defects

mature egg deposition 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

about 50%

egg production: number <50% of expected

adult lethality


egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


head shape irregular

legs pretarsus bent

antenna size decreased

antenna orientation irregular

antenna irregular

labrum position irregular

labrum size decreased

legs shape irregular

labrum irregular

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult lethality immediately after eclosion

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

vitellarium shortened

mature egg not present

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

vitellarium shortened

mature egg not present

larva number hatched none (< 10 %)

adult lethality


larva number hatched many (ca. 70-100 %)

adult lethality


head & thorax segment random number decreased

affected eggs/embryos/larvae

adult lethality

leg musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

adult lethality


adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae


adult lethality

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, no fluorescence

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality


head size decreased

procephalic head partially not present

head & thorax partially not present

head & thorax & abdomen partially not present

head partially not present

larva everted (inside-out)

appendages sometimes remaining

cuticle remnants with some identifiable segments

larval musculature pattern irregular

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched


head partially not present

head shape irregular

head & thorax partially not present

affected eggs/embryos/larvae

larval musculature pattern irregular

larval musculature fluorescence decreased

larval musculature partially not present

adult lethality


egg production: number <50% of expected

adult lethality


eclosion


embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


egg production: number <50% of expected


larva number hatched none (< 10 %)


ovary without detectable defects

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (died as pupa)


abdomen partially not present

bristle partially remaining

cuticle remnants without visible segmentation, differentiation or axis

larval musculature pattern irregular

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched


adult lethality

pupa eclosion not fulfilled (died as pupa)


adult lethality


abdomen partially not present

thorax & abdomen partially not present

abdomen partially everted (inside-out)

thorax & abdomen partially everted (inside-out)

appendages partially remaining

mostly head parts remaining

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larval musculature pattern irregular

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number few (< 50 %) hatched

adult lethality

eclosion not fulfilled (with pupal and adult features)


affected eggs/embryos/larvae

adult lethality


vitellarium mostly not present

vitellarium shortened

egg chamber mostly not present

tropharium potentially partially degenerated

egg potentially size decreased

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

egg production: number <50% of expected

egg size potentially reduced

adult lethality

adult lethality immediately after eclosion


often the head or anterior larva looks more less all right whereas the more posterior is upended.

labrum not present

many of the almost wt looking animals show a missing labrum (maybe the weakest phenotype). in some cases this comes along with a fused antenna.

head partially remaining

larval musculature potentially degenerated

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number few (< 50 %) hatched

very few


larva number hatched many (ca. 70-100 %)


affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)


larval musculature pattern irregular

affected eggs/embryos/larvae


embryo/egg not developed/not fertilized

adult lethality


larva appendages shape irregular

larva appendages shortened

legs shortened

head & thorax & abdomen partially not present

appendages partially remaining

cuticle remnants with some identifiable segments

larval musculature pattern irregular

embryo/egg not developed/not fertilized


larva segment partially random not present

only very few cuticles on the slide among many empty eggs. parts of all tagma could be missing

embryo/egg not developed/not fertilized

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

Probably no real muscle fluorescence.

larva number hatched none (< 10 %)


larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality


ovary without detectable defects

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

adult lethality


larva partially everted (inside-out)

larva everted (inside-out)

thorax & abdomen partially circumference decreased

larva complete everted (inside-out)

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


head & thorax partially not present

abdomen partially not present

cuticle remnants without visible segmentation, differentiation or axis

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

larva number few (< 50 %) hatched

adult lethality


larva partially everted (inside-out)

larva potentially ventral not present

larva appendages mostly not present

larva shape irregular

cuticle remnants without visible segmentation but ap-polarity

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larval musculature pattern irregular

vitellogenic egg chamber late number decreased

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

eclosion not fulfilled (with pupal and adult features)


adult lethality

egg production: number no/almost no eggs

adult lethality


adult lethality


embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality


gena triplet seta irregular

irregular number of gena triplet bristles. sometimes one or more are missing or duplicated.

dorsal ridge row need term irregular

dorsal ridge row bristles can be either partially missing or be of irregular size (bristles replaced by setae or vice versa)

head bristle pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larval musculature partially ectopically present

larval musculature partially not present

larval musculature potentially thinner

larval musculature pattern irregular

larval musculature potentially degenerated

larval musculature fluorescence decreased

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


head & thorax & abdomen partially not present

head partially not present

head & thorax partially not present

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

larval musculature pattern irregular

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched

adult lethality


larva number hatched many (ca. 70-100 %)

adult lethality


legs pretarsus shortened

abdomen partially not present

legs shortened

larva everted (inside-out)

adult lethality


eclosion not fulfilled (died as pupa)

adult lethality


head & thorax & abdomen segment number decreased

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae


abdomen segment number decreased

larva segment number decreased

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


larva segment random number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


cuticle partially remaining

gut remaining

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

vitellarium mostly not present

vitellarium not present

mature egg not present


legs tibiotarsus potentially fused

legs femur potentially fused

urogomphi shortened

legs shortened

maxilla shape irregular

affected eggs/embryos/larvae

leg musculature pattern irregular

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality


legs tibiotarsus potentially fused

legs femur potentially fused

legs shortened

thorax segment number decreased

head appendages partially shape irregular

head & thorax mostly not present

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

egg production: number <50% of expected


head & thorax segment number decreased

abdomen segment number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult lethality

adult lethality

adult lethality


adult lethality

adult lethality


egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

eclosion not fulfilled (with pupal and adult features)


procephalic head irregular

head & thorax & abdomen random partially not present

head random partially remaining

adult lethality


egg chamber sometimes potentially fused

vitellogenic egg chamber late not present

vitellogenic egg chamber early increased

egg production: number no/almost no eggs


adult lethality

pupa eclosion not fulfilled (died as pupa)


embryo/egg not developed/not fertilized

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

larva number hatched none (< 10 %)

adult lethality


embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

larva number hatched many (ca. 70-100 %)


adult lethality

adult lethality

adult lethality


head capsule size decreased

labium not present

mandible not present

antenna not present

labrum not present

head & thorax & abdomen everted (inside-out)

adult lethality


larva everted (inside-out)


adult lethality


ovary without detectable defects

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality immediately after eclosion


larva segment random decreased

Segment shape irregular. Appendages appear rounded.

cuticle remnants with unidentifyable segments

embryo/egg not developed/not fertilized

larval musculature irregular

larva number hatched none (< 10 %)

adult lethality


abdomen decreased

Abdomen sometimes constricted.

affected eggs/embryos/larvae

adult lethality


cuticle remnants without visible segmentation, differentiation or axis

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

affected eggs/embryos/larvae

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larval musculature decreased

larval musculature irregular

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected


head & thorax irregular

Many different defects known in the background.

affected eggs/embryos/larvae

adult lethality


affected eggs/embryos/larvae

adult lethality


vitellogenic egg chamber sometimes degenerated

egg chamber sometimes potentially fused

vitellogenic egg chamber late decreased

ovary size decreased

eclosion not fulfilled (with pupal and adult features)


mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

mature egg deposition 3 >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

adult lethality

adult lethality


abdomen segment number decreased

thorax segment number decreased

head appendages random decreased

larva segment random number decreased

cuticle remnants with some identifiable segments

larva number few (< 50 %) hatched

adult lethality

adult lethality


leg musculature decreased

The penetrance of the leg phenotype is unsure.

larval musculature pattern irregular

larval musculature decreased

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


head appendages random partially not present

larva appendages slightly shortened


adult lethality

egg production: number no/almost no eggs

adult lethality


larva anterior decreased

larva dorsal not closed

pupa eclosion not fulfilled (died as pupa)


larva number hatched many (ca. 70-100 %)

adult lethality


ovary without detectable defects

adult lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected


adult lethality


embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larval musculature lateral pattern irregular

larval musculature lateral decreased

larva number few (< 50 %) hatched


vitellogenic egg chamber late not present

adult lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


abdomen segment number decreased

head irregular

larval musculature pattern irregular

affected eggs/embryos/larvae


larva appendages shape irregular

larva segment shape irregular

abdomen bristle dorsal pattern irregular

thorax & abdomen dorsal not closed

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larval musculature irregular

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality

adult lethality


vitellogenic egg chamber position irregular

egg chamber mostly decreased

adult lethality

adult nutritional state potentially bad (cachectic)

adult lethality

adult lethality

adult lethality

adult lethality


egg chamber mostly decreased

adult lethality

adult lethality

adult lethality

vitellogenic egg chamber potentially degenerated

egg chamber potentially degenerated

mature egg mostly not present


egg chamber mostly decreased

adult lethality

adult lethality

adult lethality


larva number few (< 50 %) hatched


mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality


adult lethality

abdominal odoriferous glands less secretion


adult lethality

adult lethality

adult lethality


eclosion not fulfilled (with pupal and adult features)


adult lethality

adult lethality


larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality


larva ventral not closed

In many cases, the heads look like bent ventrally. However this might be the secondary defect of the constricted head and thorax defect.

head partially remaining

In most case it is very difficult to identify head appendages. Labium seem to be missing most of the time, while anttena, maxilla, mandible are always present. Labrum are sometimes visible, sometimes not.

head & thorax constricted

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larval musculature irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

adult lethality


tropharium posterior pattern irregular

egg chamber pattern irregular

egg chamber number decreased

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality

adult lethality

adult nutritional state bad (cachectic)


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

pupa eclosion not fulfilled (died as pupa)


larva number hatched many (ca. 70-100 %)

adult lethality


abdomen dorsal not closed

legs slightly shape irregular

larval musculature pattern irregular

Only one larva shows true muscle phenotype. Other cases seem to be obscured by segmentation defects.

larval musculature dorsal irregular

Abdomens seem to be the most sensitive.

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

adult lethality


abdomen segment number decreased

thorax segment number decreased

head appendages random decreased

cuticle remnants without visible segmentation, differentiation or axis

larval musculature irregular

larva number few (< 50 %) hatched


larva appendages slightly shortened

larva appendages shape irregular

larval musculature partially decreased

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


head & thorax & abdomen segment random partially not present

thorax & abdomen dorsal not closed

cuticle remnants with some identifiable segments

larva number few (< 50 %) hatched

adult lethality


thorax & abdomen segment decreased

larva lateral bent

head remaining

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


antenna antennal flagellum not present

antenna not present

head irregular


larval musculature decreased

larva number hatched none (< 10 %)


egg chamber number decreased

adult lethality

adult lethality


larval musculature partially decreased

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

larva number hatched none (< 10 %)

adult lethality


head partially sometimes remaining

thoracic segment partially sometimes remaining

abdomen partially remaining

embryo/egg not developed/not fertilized

larval musculature irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number hatched none (< 10 %)

adult lethality


adult lethality


eclosion not fulfilled (died as pupa)


larval musculature irregular

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (died as pupa)


larval musculature pattern irregular

affected eggs/embryos/larvae


adult lethality

adult lethality

eclosion not fulfilled (died as pupa)


adult lethality

adult lethality


adult lethality

egg production: number <50% of expected

adult lethality


adult lethality

adult lethality immediately after eclosion


adult lethality

eclosion not fulfilled (died as pupa)


head & thorax segment random not present

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


legs number increased

labium not present

abdomen segment number decreased

maxilla not present

affected eggs/embryos/larvae

adult lethality


larva number few (< 50 %) hatched


head appendages partially random not present

affected eggs/embryos/larvae

adult lethality


embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


legs shape irregular

abdomen number decreased

legs podomer number decreased

larva partially dorsal not closed

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality


thorax & abdomen segment partially potentially fused

head partially remaining

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

egg production: number no/almost no eggs

adult lethality


abdomen irregular

abdomen bent

abdomen segment number decreased

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation


follicular epithelium sometimes structure irregular

previtellogenic egg chamber structure irregular

vitellogenic egg chamber potentially degenerated

vitellogenic egg chamber shape irregular

pupa eclosion not fulfilled (died as pupa)


Besides empty eggs, there are a number of various defects known in background such as head irregular, abdominal segments decreased, dorsal closure failed, etc... making up the penetrance of about 30%.

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

larva number few (< 50 %) hatched


tropharium sometimes pattern irregular

vitellogenic egg chamber early shape irregular

vitellogenic egg chamber late not present

adult lethality


leg musculature proximal partially not present

leg musculature distal not present

leg musculature mostly not present

legs femur sometimes size increased

legs partially sometimes darker

The same defect had been obseverd in muscle prep.

larva number few (< 50 %) hatched

adult lethality

eclosion not fulfilled (with pupal and adult features)


larval musculature pattern irregular

embryo/egg not developed/not fertilized

cuticle everted (inside-out)

cuticle remnants with some identifiable segments

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg not developed/not fertilized

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number hatched none (< 10 %)


larva number hatched many (ca. 70-100 %)


tropharium structure irregular

egg production: number no/almost no eggs

adult lethality

egg chamber not present

adult lethality


leg musculature thinner

larval musculature sometimes partially shortened

larval musculature sometimes partially not present

larval musculature thinner

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


larva everted (inside-out)

abdomen posterior partially everted (inside-out)

head & thorax & abdomen segment random partially not present

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

larva number hatched none (< 10 %)

adult lethality

pupa eclosion not fulfilled (died as pupa)


adult nutritional state bad (cachectic)

adult lethality

adult lethality


cuticle remnants with unidentifyable segments

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larva number hatched none (< 10 %)

adult lethality


larval musculature pattern irregular

larva number few (< 50 %) hatched

adult lethality


embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality

adult lethality


adult lethality

prothorax segment not present

head capsule size decreased

affected eggs/embryos/larvae


abdomen segment sometimes partially fused

larval musculature dorsal pattern irregular

Sometimes the lateral musculature is also affected.

abdomen dorsal potentially not closed

abdomen ventral potentially not closed

larva number few (< 50 %) hatched

eclosion not fulfilled (with pupal and adult features)


larva potentially anteroposterior-axis shortened

egg shell potentially robustness increased

larva seta not present

larva bristle sometimes not present

larva bristle shortened

legs shape irregular

legs shortened

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

leg musculature shortened

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

egg shell potentially elasticity increased

The eggs can hardly be cracked open by squeezing.

larva number hatched none (< 10 %)

adult lethality immediately after eclosion


adult lethality

egg production: number no/almost no eggs

adult lethality

adult lethality


head partially decreased

cuticle remnants with some identifiable segments

embryo/egg without visible larval cuticle in cuticle preparation

larva number few (< 50 %) hatched

adult lethality


vitellogenic egg chamber early shape irregular

vitellogenic egg chamber late not present

ovary size decreased

Ovary size strongly reduced. Tropharia shape slightly irregular.

adult lethality


adult lethality

larva number hatched many (ca. 70-100 %)

pupa eclosion not fulfilled (died as pupa)


larva everted (inside-out)

abdomen irregular

head & thorax & abdomen segment random partially not present

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

ovary without detectable defects

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

eclosion not fulfilled (with pupal and adult features)


head & thorax & abdomen segment partially random not present

thorax & abdomen dorsal not closed

abdomen everted (inside-out)

larva everted (inside-out)

cuticle remnants without visible segmentation, differentiation or axis

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larval musculature pattern irregular

larva number hatched none (< 10 %)


adult lethality


embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality


egg chamber mostly not present

adult lethality


adult lethality

adult lethality

adult lethality


affected eggs/embryos/larvae

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

ovary without detectable defects

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected


antenna sometimes decreased

legs tibiotarsus sometimes bent

mandible not present

larval musculature pattern irregular

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


egg production: number <50% of expected

adult lethality

ovary without detectable defects

adult lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected


egg chamber complete not present

tropharium structure irregular

vitellogenic egg chamber potentially degenerated

vitellogenic egg chamber late size decreased

egg chamber mostly not present

egg production: number no/almost no eggs

adult lethality


larva number hatched none (< 10 %)


larval musculature pattern irregular


embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

egg production: number <50% of expected

ovary without detectable defects

adult lethality


mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality


larval musculature pattern irregular

larval musculature lateral thinner

There are quite a lot of dead larvae.

mature egg deposition 5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected


larva number hatched many (ca. 70-100 %)


adult lethality

larva segment partially random not present

abdomen constricted

larva everted (inside-out)

cuticle remnants with some identifiable segments

embryo/egg without visible larval cuticle in cuticle preparation

larva number few (< 50 %) hatched

adult lethality

pupa eclosion not fulfilled (died as pupa)


head irregular

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

adult lethality


adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality


adult lethality

adult lethality


embryo/egg with embryonic tissue, no cuticle, no fluorescence

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


previtellogenic egg chamber potentially not present

tropharium structure irregular

vitellogenic egg chamber sometimes potentially degenerated

egg chamber number decreased

head & thorax & abdomen segment random partially not present

cuticle remnants without visible segmentation, differentiation or axis

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

vitellogenic egg chamber late not present

larva number hatched none (< 10 %)

egg production: number <50% of expected


abdomen segment number decreased

abdomen everted (inside-out)

larval musculature pattern irregular


adult lethality

adult lethality

adult lethality immediately after eclosion


larval musculature pattern irregular

embryo/egg not developed/not fertilized

ovary without detectable defects

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected


larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality


eclosion not fulfilled (died as pupa)

adult lethality

adult lethality


head & thorax segment random partially not present

larva segment random partially not present

larva ventral irregular

larva dorsal irregular

larva number hatched many (ca. 70-100 %)


affected eggs/embryos/larvae

larva number few (< 50 %) hatched


embryo/egg not developed/not fertilized

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

larva number hatched none (< 10 %)

adult lethality

egg production: number <50% of expected

adult lethality


embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


abdomen segment number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

eclosion not fulfilled (died as pupa)


affected eggs/embryos/larvae

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

eclosion not fulfilled (with pupal and adult features)


adult lethality


adult lethality

egg production: number no/almost no eggs

adult lethality


abdomen segment number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


head & thorax segment number decreased

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality

adult lethality

adult cuticle colour lighter

adult lethality


head & thorax segment number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)

adult lethality


head appendages partially not present

thorax mostly not present

head size decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality


abdominal odoriferous glands size decreased

abdominal odoriferous glands darker

tropharium size decreased

tropharium shape irregular

vitellarium not present

egg chamber not present


head size decreased

larva segment number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

eclosion not fulfilled (died as pupa)


adult lethality

adult lethality

adult lethality


abdomen segment number # of remaining segments  decreased

posterior terminus present

thorax segment partially not present

head appendages random not present

affected eggs/embryos/larvae

adult lethality

adult lethality

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

adult lethality

eclosion not fulfilled (died as pupa)


adult lethality

adult lethality


head & thorax segment number decreased

cuticle remnants strongly fragmented (cuticle crumbs)

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

larva sometimes everted (inside-out)

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae


adult lethality

abdominal odoriferous glands less secretion


mandible not present

antenna not present

abdomen segment number decreased

thorax segment number decreased

head size decreased

head & thorax & abdomen segment number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality


posterior terminus present

affected eggs/embryos/larvae

larval musculature fluorescence decreased

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


larval musculature thinner

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality

adult lethality immediately after eclosion

larva number hatched many (ca. 70-100 %)


adult lethality


mature egg deposition 4 5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality


head & thorax segment number decreased

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

eclosion not fulfilled (died as pupa)


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched


leg musculature swollen

Leg musculuature seems thicker, specifically seen in the coxa.

leg musculature shape irregular

head musculature potentially swollen

Musculature seems thicker.

head musculature fluorescence increased

affected eggs/embryos/larvae

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

adult lethality

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva partially dorsal not closed

head & thorax & abdomen random not present

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


head capsule mostly size decreased

head appendages random not present

affected eggs/embryos/larvae


adult lethality

adult lethality


head constricted

In strong cases, the head is completely seperated from the thorax.

head & thorax & abdomen segment random not present

Head and thorax are more often affected than the abdomen.

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


abdomen not present

metathorax not present

affected eggs/embryos/larvae


head & thorax & abdomen segment random mostly not present

head & thorax segment random not present

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


larva number few (< 50 %) hatched


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

egg production: number <50% of expected

adult lethality


mature egg deposition 4 >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number no/almost no eggs

adult lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


larva dorsal pattern irregular

larva constricted

Mostly between head and thorax.

thorax constricted

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality


egg production: number <50% of expected

adult lethality


affected eggs/embryos/larvae

larva number hatched none (< 10 %)

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality


head appendages potentially structure fused

antenna number decreased

head capsule size decreased

head appendages orientation irregular

mesothorax circumference decreased

labrum size decreased

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

adult lethality


larva everted (inside-out)

affected eggs/embryos/larvae

affected eggs/embryos/larvae


abdominal odoriferous glands mostly content not present

larva number hatched many (ca. 70-100 %)


larva dorsal potentially pattern irregular

posterior terminus shape irregular

In strong cases the head is completely absent.

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

larval musculature fluorescence not present

Potentially due to death.

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality


eclosion not fulfilled (with pupal and adult features)


head appendages sometimes partially not present

thorax & abdomen segment random not present

In strong cases only the head is remaining.

cuticle remnants strongly fragmented (cuticle crumbs)

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

embryo/egg not developed/not fertilized

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality


abdomen bristle potentially pattern irregular

Potentially secondary defect due to the squeezed segments.

abdomen segment dorsal dorsalized

All abdominal segments are present, but dorsal part of the segments are squeezed together.

affected eggs/embryos/larvae

larval musculature random not present

Predominatly is the abdominal musculature affected.

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva partially everted (inside-out)

larva complete everted (inside-out)

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

larval musculature pattern irregular

affected eggs/embryos/larvae


larva partially dorsal not closed

head & thorax & abdomen segment random not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality


egg production: number <50% of expected

ovary without detectable defects

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality


head capsule structure irregular

antenna not present

antenna antennal flagellum not present

antenna pedicellus not present

abdomen number decreased

antenna size decreased

larva partially dorsal not closed

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


larval musculature pattern irregular

affected eggs/embryos/larvae


affected eggs/embryos/larvae

larval musculature dorsal not fused

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality


head appendages partially potentially position irregular

labrum potentially size decreased

abdomen segment number decreased

head irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)

adult lethality

larva dorsal not closed

larva dorsal pattern irregular

abdomen segment number decreased

head & thorax random partially not present

affected eggs/embryos/larvae

larval musculature posterior pattern increased

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


larva partially everted (inside-out)

larva everted (inside-out)

affected eggs/embryos/larvae

adult lethality


cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

larval musculature fluorescence degenerated

affected eggs/embryos/larvae

larva number hatched many (ca. 70-100 %)

adult lethality