adult lethality

larva lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


fertility after larval injection decreased (no living offspring)

larva lethality

adult lethality

abdomen posterior everted (inside-out)

abdomen segment number decreased

legs tibiotarsus size decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality

larva number hatched many (ca. 70-100 %)

adult lethality


eclosion delayed (still pupa)

eclosion not fulfilled (died as pupa)

larva lethality

adult lethality

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

abdominal odoriferous glands potentially size decreased

odoriferous glands darker

head & thorax & abdomen segment number decreased

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

abdominal odoriferous glands secretion color lighter


eclosion not fulfilled (died as pupa)

larva lethality

odoriferous glands darker

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

affected eggs/embryos/larvae


adult lethality

larva lethality

adult lethality


larva lethality

adult lethality

legs shape irregular

abdomen segment number decreased

thorax segment number decreased

head appendages partially orientation irregular

larva segment number decreased

head partially remaining

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, no fluorescence

embryo/egg not developed/not fertilized

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality


adult lethality

larva lethality


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

adult lethality


larva lethality

adult lethality

adult lethality


pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

adult lethality


oblique dorsal thoracic muscles distal size shortened

elytra shortened

larva lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

vitellogenic egg chamber late number increased

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (with pupal and adult features)


pupal molt not fulfilled (died as prepupa)

adult lethality

egg chamber sometimes structure irregular

vitellogenic egg chamber not present

mature egg not present

larva number hatched none (< 10 %)

egg production: number no/almost no eggs


eclosion not fulfilled (died as pupa)

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected


fertility after larval injection decreased (no living offspring)

eclosion not fulfilled (died as pupa)

larva lethality

adult lethality

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


eclosion not fulfilled (died as pupa)

pupa fluorescence not present

no GFP fluorescence in the whole pupa. also no red fluorescence visible. maybe cuticle is thicker than normal

wings not present

legs partially size shortened

antenna orientation irregular

abdomen gin-traps size shortened

legs partially not present

labrum orientation irregular

pupal molt not completely molted

larva lethality

adult lethality

antenna circumference increased

affected eggs/embryos/larvae

larval musculature number decreased

larval musculature pattern irregular

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva lethality

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

thorax dorsal not closed

thorax partially not present

head appendages partially not present

larva anterior irregular

affected eggs/embryos/larvae

adult lethality

larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

legs pretarsus swollen

antenna shape irregular

affected eggs/embryos/larvae

adult lethality

larval musculature lateral number decreased

leg musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


adult lethality

eclosion not fulfilled (not completely eclosed)

eclosion not fulfilled (died as pupa)

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

adult lethality


ovary without detectable defects

egg production: number <50% of expected

eclosion delayed (still pupa)

eclosion delayed (still pupa)

legs femur size shortened

femur of all three leg-pairs shortened

pupal molt delayed (still larva)

larva lethality


adult lethality

larva lethality

adult lethality


adult lethality

funicle not present

maxillary palp slightly size elongated

hindwing necrotic

legs tibia potentially fused

legs tibia proximal division boundary not present

legs shortened

legs tarsus not present

Pretarsus present!!! Most distal part of appendages present!!!

legs swollen

ommatidia number decreased

labrum shortened

labial palp swollen

maxillary palp elongated

female genital lobes rounded

legs shortened

antenna orientation irregular

adult lethality

affected eggs/embryos/larvae

leg musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

ovary without detectable defects

tropharium shortened

mature egg deposition 3 5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


eclosion not fulfilled (died as pupa)

adult lethality

adult lethality


pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality

adult nutritional state bad (cachectic)

adult lethality

head & thorax & abdomen segment number decreased

abdomen segment number decreased

head appendages random number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

larval musculature pattern irregular

affected eggs/embryos/larvae


eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality


larva lethality


ovary without detectable defects

egg production: number <50% of expected

adult lethality

larva lethality

abdomen segment anterior number decreased

thorax segment number decreased

head appendages partially not present

larva anterior decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

legs shortened

head size decreased

head capsule not present

head & thorax not present


eclosion not fulfilled (died as pupa)

eclosion not fulfilled (died as pupa)

head appendages partially not present

legs shortened

head appendages partially shape irregular

cuticle remnants without visible segmentation but ap-polarity

affected eggs/embryos/larvae

egg production: number >150% of expected


larva lethality

thorax segment number decreased

legs femur potentially fused

legs tibiotarsus potentially fused

abdomen segment number decreased

legs sometimes shortened

labium not present

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still prepupa)

larva lethality

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


pupal molt not fulfilled (died with pupal and larval features)

pupal molt delayed (still larva)

larva lethality

adult lethality

larva number hatched many (ca. 70-100 %)


adult lethality

adult lethality immediately after eclosion

larva lethality


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

adult lethality


larva lethality

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

adult lethality

adult lethality

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

larva lethality

thorax segment number decreased

abdomen segment number decreased

legs number increased

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

larva lethality

thorax segment number decreased

abdomen segment number decreased

legs number increased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

head & thorax & abdomen segment random not present

head & thorax & abdomen segment shape irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected


adult lethality


fertility after larval injection decreased (no living offspring)

adult lethality

eclosion not fulfilled (died as pupa)

head & thorax & abdomen segment partially not present

abdomen partially remaining

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched


adult lethality

eclosion not fulfilled (not completely eclosed)

larva lethality

eclosion not fulfilled (with pupal and adult features)


eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

adult lethality


eclosion not fulfilled (died as pupa)

adult lethality

hindwing blistered

elytra surface irregular

eclosion delayed (still pupa)

elytra not closed

pupal molt delayed (still larva)

larva lethality

adult lethality

adult nutritional state potentially bad (cachectic)

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality


adult lethality

larva lethality

head & thorax & abdomen segment mostly not present

2 animals with phenotype; rest empty eggs

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


eclosion not fulfilled (died as pupa)

abdomen gin-trap setae size shortened

pronotum bristle size shortened

larva lethality

adult lethality


adult lethality

elytra size shortened

elytra surface irregular

eclosion not fulfilled (died as pupa)

elytra not closed

longitudinal dorsal thoracic muscles fluorescence decreased

oblique dorsal thoracic muscles shortened

wings size decreased

wings not closed

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected


adult lethality

adult lethality

larva lethality

larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

adult lethality immediately after eclosion

adult lethality


eclosion not fulfilled (died as pupa)

wings shape rounded

eclosion not fulfilled (died as pupa)

wings size shortened

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality

legs tibiotarsus size decreased

affected eggs/embryos/larvae

larval musculature fluorescence not present

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


eclosion delayed (still pupa)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still larva)

larva lethality

legs shape irregular

head appendages shape irregular

legs shortened

head appendages partially not present

affected eggs/embryos/larvae

adult lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality


adult lethality

hindwing folding irregular

elytra surface irregular

elytra not closed

adult lethality

larva lethality

affected eggs/embryos/larvae

adult lethality

embryo/egg with embryonic tissue, no cuticle, no fluorescence

50% are affected.

affected eggs/embryos/larvae

adult lethality

adult lethality


adult lethality

hindwing folding irregular

elytra surface irregular

elytra not closed

adult lethality

larva lethality

affected eggs/embryos/larvae

adult lethality

50% are affected.

affected eggs/embryos/larvae

adult lethality

adult lethality


larva lethality


larva lethality

adult lethality


larva lethality

adult lethality

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

adult lethality


eclosion not fulfilled (died as pupa)

adult lethality


eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

larva lethality

adult lethality


adult lethality


pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still prepupa)

eyes size decreased

pupal molt delayed (still larva)

larva lethality

affected eggs/embryos/larvae

adult lethality

mature egg not present

previtellogenic egg chamber potentially present

potentially some previtellogenic eggs in posterior region of tropharium

vitellogenic egg chamber not present

vitellarium not present

larva number hatched none (< 10 %)


fertility after larval injection decreased (no living offspring)

ovary without detectable defects

egg production: number <50% of expected

adult nutritional state bad (cachectic)

eclosion not fulfilled (not completely eclosed)

eclosion not fulfilled (died as pupa)

larva lethality

adult lethality

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality late (L7)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

larva lethality

affected eggs/embryos/larvae

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality

head & thorax & abdomen segment number decreased

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


eclosion not fulfilled (died as pupa)

adult lethality

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality


adult lethality

larva lethality

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

eclosion not fulfilled (died as pupa)

wings not closed

wings size shortened

pupal molt delayed (still larva)

larva lethality

adult lethality

antenna not present

labrum not present

larva cuticle ventral partially not closed

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

adult nutritional state potentially bad (cachectic)

larva number hatched none (< 10 %)


adult lethality

pupal molt delayed (still larva)

larva lethality

abdomen segment number decreased

thorax segment number decreased

labium not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


larva lethality

larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

larva lethality

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched


eclosion not fulfilled (died as pupa)

larva lethality late (L7)

antenna size shortened

oblique dorsal thoracic muscles partially fragmented

wings shape irregular

wings not closed

eclosion not fulfilled (died as pupa)

female genital lobes rounded

oblique dorsal thoracic muscles partially not present

wings size shortened

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality

adult nutritional state bad (cachectic)

adult lethality


eclosion not fulfilled (died as pupa)


tropharium shape irregular

tropharium size shortened

vitellogenic egg chamber not present

previtellogenic egg chamber not present

vitellarium not present

egg production: number <50% of expected

larva lethality

affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva lethality late (L7)

pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

larva lethality

adult lethality

gut remaining

potentially just hindgut

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

adult lethality


tropharium shape irregular

tropharium size shortened

egg production: number <50% of expected

larva lethality

adult lethality

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

adult lethality


adult lethality

adult lethality

elytra slightly size shortened

elytra surface irregular

elytra not closed

oblique dorsal thoracic muscles fragmented

the outer oblique muscles is fragmented, the median one is ok

wings slightly size shortened

larva lethality

adult lethality

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected


eclosion not fulfilled (died as pupa)

elytra surface irregular

larva lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality


eclosion not fulfilled (died as pupa)

elytra surface irregular

larva lethality

adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality

adult lethality


larva lethality

adult lethality


pupal molt not fulfilled (died as L7 or prepupa)

larva lethality

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

adult lethality

affected eggs/embryos/larvae

larval musculature mostly not present

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)


pupal molt delayed (still prepupa)

larva lethality

legs femur fused

legs femur shortened

legs tibiotarsus fused

legs tibiotarsus shortened

affected eggs/embryos/larvae

adult lethality

larval musculature lateral sometimes orientation irregular

leg musculature distal pattern irregular

larval musculature lateral number decreased

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

eclosion not fulfilled (with pupal and adult features)


larva lethality

adult lethality

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

adult lethality

larva number few (< 50 %) hatched

adult lethality


fertility after larval injection decreased (no living offspring)

vitellogenic egg chamber not present

previtellogenic egg chamber mostly not present

egg production: number <50% of expected

adult lethality

larva lethality

maxillary palp present

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

ovary without detectable defects

tropharium size decreased

vitellarium not present

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

adult lethality

larva number hatched many (ca. 70-100 %)


adult nutritional state bad (cachectic)

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

adult lethality immediately after eclosion

pupal molt delayed (still larva)

larva lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


motility decreased

adult lethality

adult nutritional state bad (cachectic)

motility decreased

eclosion not fulfilled (died as pupa)

larva lethality

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult nutritional state bad (cachectic)

adult lethality


eclosion not fulfilled (died as pupa)

adult lethality

eclosion delayed (still pupa)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

adult lethality

adult lethality

labrum size decreased

mandible potentially transformed

mandible not present

maxilla number increased

affected eggs/embryos/larvae

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality


eclosion not fulfilled (died as pupa)

abdomen segment circumference decreased

pupal molt delayed (still larva)

larva lethality


eclosion not fulfilled (died as pupa)

larva lethality

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality late (L7)

pupal molt not fulfilled (died with pupal and larval features)

oblique dorsal thoracic muscles median size broader

legs orientation irregular

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality

adult nutritional state bad (cachectic)


adult lethality

elytra size shortened

elytra surface irregular

eclosion not fulfilled (not completely eclosed)

eclosion not fulfilled (died as pupa)

elytra not closed

oblique dorsal thoracic muscles fragmented

the outer one of the two oblique muscles is fragented, the median one looks normal

wings size shortened

larva lethality

adult lethality


larva lethality


larva lethality

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

abdomen segment number decreased

legs distal decreased

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

eclosion not fulfilled (died as pupa)

affected eggs/embryos/larvae

adult lethality

larval musculature fluorescence decreased

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

ovary without detectable defects

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected


eclosion not fulfilled (died as pupa)

larva lethality


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

larva lethality

adult lethality


larva lethality

adult lethality

adult lethality


adult lethality

pupal molt delayed (still larva)

larva lethality

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

adult lethality

egg production: number <50% of expected


adult lethality

adult nutritional state bad (cachectic)

adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected


eclosion not fulfilled (died as pupa)

eyes size decreased

dorsal thoracic muscles partially rounded

legs tarsus shape irregular

legs orientation irregular

labrum size decreased

labrum orientation irregular

wings blistered

dorsal thoracic muscles partially not present

wings size shortened

antenna orientation irregular

pupal molt not fulfilled (died as prepupa)

larva lethality

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


adult lethality

adult lethality

adult lethality

hindwing folding irregular

elytra slightly size shortened

eclosion not fulfilled (died as pupa)

elytra surface irregular

wings not closed

wings slightly size shortened

larva lethality

head partially size decreased

adult lethality

thorax dorsal not closed

antenna partially size decreased

head & thorax & abdomen segment number decreased

affected eggs/embryos/larvae

larval musculature degenerated

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

eclosion not fulfilled (with pupal and adult features)


eclosion not fulfilled (died as pupa)

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality


adult lethality

larva lethality

labrum size decreased

mandible potentially transformed

mandible not present

maxilla number increased

affected eggs/embryos/larvae

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality


fertility after larval injection decreased (no living offspring)

adult lethality

eclosion not fulfilled (died as pupa)

adult lethality

adult lethality


adult lethality

adult lethality

adult lethality

elytra not closed

adult lethality

larva lethality

legs segment swollen

antenna distal swollen

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality


larva lethality

adult lethality

adult lethality

adult lethality

adult lethality


adult lethality

larva lethality late (L7)

larva lethality

adult lethality


larva lethality late (L7)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

egg production: number <50% of expected


larva lethality

egg production: number >150% of expected


larva lethality

adult lethality


adult lethality

larva lethality


adult lethality

egg production: number <50% of expected

adult nutritional state bad (cachectic)

adult lethality

larva lethality

adult lethality


adult lethality

adult lethality

adult lethality


adult lethality

adult lethality

adult lethality

adult lethality


adult lethality

adult lethality


larva lethality


larva lethality

mandible potentially everted (inside-out)

legs partially decreased

mandible not present

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

larval musculature partially shape irregular

larval musculature partially not present

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

larva number hatched many (ca. 70-100 %)


adult lethality

eclosion not fulfilled (died as pupa)

elytra not closed

wings size decreased

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

eclosion not fulfilled (died as pupa)


larva lethality

adult lethality

egg production: number no/almost no eggs

adult lethality


adult lethality

adult lethality

elytra not present

adult lethality

pronotum shape irregular

abdomen gin-traps not present

dorsal thoracic muscles not present

wings not present

head orientation irregular

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

egg chamber shape irregular

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


larva lethality

head size decreased

head appendages sometimes orientation irregular

legs partially distal decreased

head appendages sometimes shape irregular

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


larva lethality

affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected


larva lethality

affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


larva lethality

adult lethality

adult lethality

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality


pupal molt delayed (still larva)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

vitellarium not present

adult lethality

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality


adult lethality

adult lethality

mature egg deposition 3 >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

adult nutritional state potentially bad (cachectic)

adult lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)


larva lethality

adult lethality


adult lethality

larva lethality

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae


adult lethality

adult lethality

adult lethality


larva lethality

legs partially shortened

abdomen segment number decreased

thorax segment number decreased

labium not present

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult lethality

adult lethality

adult lethality

adult lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larval musculature fluorescence decreased

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

larva lethality

adult lethality

adult lethality


adult lethality

adult nutritional state

elytra blistered

eclosion delayed (still pupa)

elytra not closed

oblique dorsal thoracic muscles thinner

wings not closed

larva lethality

adult lethality

head musculature partially rounded

head musculature number decreased

head musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

larva lethality

adult lethality


fertility after larval injection decreased (no living offspring)

ovary without detectable defects

egg production: number <50% of expected

larva lethality

adult lethality

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality

larva lethality


larva lethality


head appendages partially not present

legs swollen

head segmentation/division not present

affected eggs/embryos/larvae

hindgut everted (inside-out)

legs shortened

head appendages shape irregular

thorax & abdomen dorsoventral-axis thinner

adult lethality

larval musculature pattern irregular

larva number hatched none (< 10 %)

adult lethality


larva lethality

adult lethality

adult lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

adult lethality


eclosion not fulfilled (died as pupa)

pupal molt not fulfilled (died as L7 or prepupa)

pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

adult lethality

adult nutritional state bad (cachectic)


adult lethality

elytra not closed

eclosion not fulfilled (died as pupa)

elytra surface irregular

wings blistered

larva lethality

adult lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

adult lethality


larva lethality

adult lethality

adult lethality


eclosion not fulfilled (died as pupa)

pupal molt delayed (still prepupa)

oblique dorsal thoracic muscles lateral decreased

oblique dorsal thoracic muscles shortened

oblique dorsal thoracic muscles orientation irregular

wings mostly decreased

head orientation irregular

pupal molt delayed (still larva)

larva lethality

legs pretarsus not fused

abdomen mostly not present

legs segment fused

head mostly not present

affected eggs/embryos/larvae

egg production: number no/almost no eggs

adult lethality

adult lethality

larva number hatched none (< 10 %)

adult lethality


adult lethality

larva number hatched many (ca. 70-100 %)

adult lethality


larva lethality

adult lethality

adult lethality

adult lethality


adult lethality

motility decreased

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


pupa size decreased

longitudinal dorsal thoracic muscles posterior position irregular

pupa bristle mostly not present

larva lethality

adult lethality

larval musculature partially not present

larval musculature mostly thinner

affected eggs/embryos/larvae

adult nutritional state potentially bad (cachectic)

adult lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality

adult lethality


adult lethality

adult lethality

adult lethality


adult lethality

larva lethality

adult lethality


adult lethality

adult lethality

adult lethality

larva lethality

adult lethality

adult lethality

adult lethality

adult lethality


adult lethality

adult lethality


adult lethality

adult lethality

wings posterior bent

adult lethality

egg production: number <50% of expected


pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

larva number hatched none (< 10 %)


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)


adult lethality

adult lethality

eclosion not fulfilled (not completely eclosed)

larva lethality

adult lethality


adult lethality

adult lethality

larva lethality

adult lethality


abdominal odoriferous glands partially colour darker

ovary without detectable defects

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (died as pupa)

head appendages sometimes not present

legs partially shape irregular

head appendages partially shape irregular

larva appendages shape irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


eclosion not fulfilled (died as pupa)

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

adult lethality

motility decreased

adult lethality

adult lethality immediately after eclosion

larva lethality

urogomphi size decreased

affected eggs/embryos/larvae

adult lethality

adult lethality

larva number few (< 50 %) hatched

adult lethality


adult lethality

larva lethality

head & thorax segment number decreased

adult lethality

head & thorax & abdomen segment number decreased

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


fertility after larval injection decreased (no living offspring)

ovary without detectable defects

egg production: number <50% of expected

eclosion not fulfilled (died as pupa)

adult lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


eclosion delayed (still pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

adult lethality


larva lethality

adult lethality


larva lethality

larva cuticle duplicated

L1 larva cuticle visible under L2 larva cuticle

affected eggs/embryos/larvae

adult lethality

mature egg deposition 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


adult lethality

tropharium size decreased

egg production: number <50% of expected

adult lethality

adult lethality

hindwing folding irregular

eclosion not fulfilled (died as pupa)

elytra not closed

adult lethality

previtellogenic egg chamber number decreased

tropharium shortened

ovary without detectable defects

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


abdominal odoriferous glands colour lighter

abdominal glands appear lighter with one dark spot inside

abdominal odoriferous glands spotted

prothoracic odoriferous glands colour darker

adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

affected eggs/embryos/larvae

prothoracic odoriferous glands darker

abdominal odoriferous glands darker

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva lethality

larva number hatched many (ca. 70-100 %)


adult lethality

adult lethality

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

abdomen segment potentially transformed

abdominal segment 2 tracheal opening not present

legs number increased

abdomen segment number decreased

thorax segment partially not present

larva segment number decreased

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


larva lethality

eclosion not fulfilled (died as pupa)

egg production: number >150% of expected


larva lethality

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality


larva lethality

adult lethality

larva appendages shape irregular

larva median constricted

affected eggs/embryos/larvae

adult lethality


eclosion not fulfilled (died as pupa)

adult lethality

larva constricted

abdomen segment number decreased

thorax segment number decreased

head appendages partially not present

larva segment number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

eclosion not fulfilled (died as pupa)

egg production: number <50% of expected

adult lethality


adult lethality

larva lethality

adult lethality

adult lethality


adult lethality

larva lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (died as pupa)


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality

adult lethality

eclosion not fulfilled (not completely eclosed)

larva lethality

mandible not present

head capsule not present

labrum not present

antenna not present

head appendages partially not present

affected eggs/embryos/larvae

adult lethality


eclosion delayed (still pupa)

pupal molt delayed (still larva)

adult lethality


eclosion delayed (still pupa)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

larva lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

egg production: number <50% of expected

labrum size decreased

mandible potentially transformed

mandible not present

maxilla number increased

affected eggs/embryos/larvae

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality

adult lethality

adult lethality


larva lethality

adult lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

adult lethality

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


eclosion not fulfilled (with pupal and adult features)

eclosion not fulfilled (died as pupa)

larva lethality

adult lethality

adult lethality


larva lethality

affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

eclosion delayed (still pupa)

eclosion delayed (still pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

adult lethality

labrum size decreased

mandible potentially transformed

mandible not present

maxilla number increased

affected eggs/embryos/larvae

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality


larva lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

larva lethality

larva number few (< 50 %) hatched

adult lethality


larva lethality

adult lethality


ovary without detectable defects

egg production: number <50% of expected

eclosion not fulfilled (died as pupa)

larva lethality

adult lethality

mature egg not present

egg production: number no/almost no eggs


adult lethality

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

adult lethality

adult lethality

abdomen segment number decreased

affected eggs/embryos/larvae

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

adult lethality

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae


eclosion delayed (still pupa)

adult lethality

larva lethality

legs tibiotarsus shape irregular

legs pretarsus duplicated

thorax segment partially not present

head appendages partially not present

head & thorax partially not present

affected eggs/embryos/larvae

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

adult lethality


adult lethality

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


abdominal odoriferous glands size decreased

adult lethality

eclosion not fulfilled (died as pupa)

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

adult lethality immediately after eclosion

pupal molt delayed (still larva)

adult lethality


eclosion not fulfilled (died as pupa)

adult lethality

larva lethality

adult lethality

egg production: number <50% of expected


pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

larva lethality

adult lethality

mandible not present

antenna not present

labrum not present

legs partially shape irregular

mesothoracic leg femur duplicated

abdomen segment number decreased

abdomen complete not present

mesothorax not present

head capsule not present

larva segment number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


eclosion not fulfilled (died as pupa)

larva lethality


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva lethality

larva number few (< 50 %) hatched

adult lethality


eclosion not fulfilled (died as pupa)

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


larva lethality

adult lethality

adult lethality


pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality


adult lethality

adult lethality

adult nutritional state bad (cachectic)

motility decreased

larva lethality

antenna shape irregular

affected eggs/embryos/larvae

ovary robustness decreased

very hard to preperate. ovaries disintegrated quickly

egg chamber number decreased

vitellarium shortened

tropharium shortened

larva number hatched none (< 10 %)

egg production: number <50% of expected


pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still prepupa)

adult lethality

adult lethality

adult nutritional state potentially bad (cachectic)

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

larva lethality

vitellarium shortened

tropharium shortened

adult lethality

egg chamber not present

adult lethality

adult lethality


fertility after larval injection decreased (no living offspring)

eclosion not fulfilled (died as pupa)

larva lethality

adult lethality


larva lethality

affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva lethality

head & thorax segment number decreased

cuticle remnants strongly fragmented (cuticle crumbs)

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


eclosion not fulfilled (died as pupa)

larva lethality

head appendages partially shape irregular

abdomen partially everted (inside-out)

legs partially shortened

head appendages random not present

larva appendages partially irregular

affected eggs/embryos/larvae

larva number hatched many (ca. 70-100 %)


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

larva number few (< 50 %) hatched

adult lethality


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

eclosion not fulfilled (died as pupa)

abdomen segment circumference decreased

abdomen size decreased

all pupae showed the decreased abdomen. 3 females were adult at this timepoint and 1 was still prepupa. so the number of analyzable females for this phenotype were 6.

pupal molt delayed (still prepupa)

larval musculature fluorescence decreased

larval musculature pattern irregular

affected eggs/embryos/larvae

egg production: number <50% of expected

ovary without detectable defects

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

adult lethality


eclosion not fulfilled (died as pupa)

pronotum shape irregular

pronotum size decreased

eyes surface opaque

eye surface seems to be still covered by a membrane

eclosion delayed (still pupa)

wings blistered

eyes potentially size decreased

pupa size decreased

larva lethality

larval musculature sometimes partially shortened

larval musculature sometimes partially not present

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

adult lethality

eclosion not fulfilled (died as pupa)


larva lethality


larva lethality early (L5/6)

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

eclosion


eclosion delayed (still pupa)

head capsule partially not present

legs partially not present

abdomen partially irregular

legs sometimes irregular

head appendages partially not present

head & thorax & abdomen segment partially not present

affected eggs/embryos/larvae

50% are affected.

eclosion

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality early (L5/6)

adult lethality

adult lethality


larva lethality

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (died as pupa)

embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

embryo/egg not developed/not fertilized


eclosion not fulfilled (died as pupa)

hindwing potentially necrotic

longitudinal dorsal thoracic muscles potentially partially not present

oblique dorsal thoracic muscles partially not present

wings blistered

pupal molt not completely molted

larva lethality

adult lethality

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected


egg chamber potentially ectopically present

yolk sometimes ectopically present

egg chamber mostly not present

tropharium structure irregular

nurse cells seem to be smaller

tropharium size decreased

ovary size decreased

vitellarium not present

no follicle cells seem to be present

vitellarium size decreased

egg production: number no/almost no eggs

elytra wing veins slightly decreased

in addition, the pattern of sensilla seems to be less regular

eclosion not fulfilled (died as pupa)

elytra surface irregular

adult slightly size decreased

larva lethality late (L7)

elytra ventral slightly not closed

ovariole degenerated

vitellogenic egg chamber early shape irregular

vitellogenic egg chamber early potentially degenerated

tropharium potentially size decreased

vitellogenic egg chamber late not present

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected


larva lethality

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (died as pupa)


adult lethality immediately after eclosion

larva lethality

larva number hatched many (ca. 70-100 %)

adult lethality


larva lethality late (L7)

larva lethality early (L5/6)

adult lethality

adult lethality


larva lethality

adult lethality


larva lethality late (L7)

pupal molt not fulfilled (died with pupal and larval features)

pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still larva)

larva lethality early (L5/6)

adult lethality

adult lethality

eclosion not fulfilled (died as pupa)


wings shortened

antenna unilateral orientation irregular

larva lethality

larva number few (< 50 %) hatched

adult lethality

eclosion not fulfilled (with pupal and adult features)

eclosion not fulfilled (died as pupa)


wings ventral not closed

wings slightly rounded

wings slightly size decreased

legs slightly orientation irregular

head irregular

the head is translucent in transmitted light, although the ds-red expression in the cns is comparable to WT

larva lethality

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larval musculature pattern irregular

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (died as pupa)


larva lethality late (L7)

eclosion not fulfilled (died as pupa)

cannibalized, not fully analyzable

pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality

tropharium structure irregular

Anterior parts seem to be more affected.

vitellarium potentially not present

egg chamber not present

ovary size decreased

Dramatically decreased.

adult lethality


pupal molt delayed (still larva)

adult lethality

eclosion not fulfilled (died as pupa)


pupal molt delayed (still larva)

adult lethality

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

larva random constricted

larva segment partially not present

cuticle remnants with some identifiable segments

Many are normal morphologically but do not hatch.

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number hatched none (< 10 %)

adult lethality


fertility after larval injection decreased (no living offspring)

larva lethality

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


larva lethality

larva segment partially not present

cuticle remnants with unidentifyable segments

embryo/egg not developed/not fertilized

larval musculature pattern irregular

larva number hatched none (< 10 %)

adult lethality


adult lethality

head slightly orientation irregular

adult lethality

eclosion not fulfilled (died as pupa)


eclosion delayed (still pupa)

larva lethality

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

adult lethality


larva lethality

adult lethality

eclosion not fulfilled (died as pupa)


eclosion not fulfilled (died as pupa)

pupal molt not fulfilled (died as L7 or prepupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality

embryo/egg not developed/not fertilized

larval musculature pattern irregular

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (died as pupa)


adult lethality

larva lethality

mandible not present

In few cases, labrum, maxilla or labium are missing.

larval musculature pattern irregular

larva number few (< 50 %) hatched


adult lethality

larva lethality

affected eggs/embryos/larvae

adult lethality


eclosion not fulfilled (died as pupa)

adult lethality

eclosion delayed (still pupa)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

adult lethality

adult lethality


adult lethality

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

mature egg deposition 2 5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality

eyes potentially slightly size decreased

ommatidia partially pigmentation/tanning not present

outer ring of ommatidia lacks black pigmentation

larva segment ventral potentially fused

larval musculature lateral partially not present

larval musculature ventral partially not present

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

larva number hatched none (< 10 %)

eclosion not fulfilled (with pupal and adult features)

eclosion not fulfilled (died as pupa)


larva lethality

gena triplet seta number increased

larval musculature pattern irregular

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (died as pupa)


eclosion not fulfilled (died as pupa)

larva lethality

abdomen shape irregular

legs shortened

labial palp ventral position not fused

abdomen gin-traps size decreased

wings not present

antenna shortened

pupal molt not fulfilled (died with pupal and larval features)

legs trochanter not present

In few cases trochanter are strongly shortened.

legs shortened

Pretarsus are not slightly bent as usual.

legs tibiotarsus potentially fused

legs femur potentially fused

labrum not present

Antennae are sometimes shortened.

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

leg musculature shortened

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

tropharium potentially size decreased

egg chamber not present

adult lethality

adult nutritional state potentially bad (cachectic)

egg production: number no/almost no eggs


eclosion delayed (still pupa)

larva number hatched many (ca. 70-100 %)

adult lethality


vitellogenic egg chamber late slightly number decreased

previtellogenic egg chamber late potentially degenerated

vitellogenic egg chamber late partially structure irregular

egg production: number <50% of expected

adult lethality

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

larval musculature sometimes partially not present

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (died as pupa)


adult lethality

adult lethality

eclosion delayed (still pupa)

adult lethality

larva lethality

larva number few (< 50 %) hatched

adult lethality


eclosion not fulfilled (died as pupa)

potentially cannibalized by adults

larva lethality

abdomen everted (inside-out)

thorax & abdomen everted (inside-out)

Thorax sometimes patially upended.

cuticle everted (inside-out)

cuticle remnants without visible segmentation, differentiation or axis

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larval musculature fluorescence decreased

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larva number hatched none (< 10 %)


eclosion delayed (still pupa)

eclosion delayed (still pupa)

adult lethality

adult lethality


eclosion delayed (still pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

adult lethality


eclosion not fulfilled (died as pupa)

pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still larva)


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

adult lethality


flagellomeres number decreased

all animals show 8 instead of 10 flagellomeres


pupal molt delayed (still larva)

head partially not present

head & thorax & abdomen partially not present

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult nutritional state bad (cachectic)

adult lethality


fertility after larval injection decreased (no living offspring)

abdomen partially not present

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larval musculature partially not present

larval musculature pattern irregular

embryo/egg not developed/not fertilized

about 25%

larva number few (< 50 %) hatched

very few

adult lethality

pupa eclosion not fulfilled (died as pupa)


larva lethality

larva number hatched many (ca. 70-100 %)


larva lethality

head partially not present

head shape irregular

appendages sometimes remaining

cuticle remnants with unidentifyable segments

adult lethality


pupal molt delayed (still larva)

larva lethality

egg production: number no/almost no eggs

adult lethality

adult lethality


larva lethality

adult lethality


eclosion not fulfilled (died as pupa)

pupal molt delayed (still prepupa)

pupal molt not fulfilled (died as L7 or prepupa)

vitellogenic egg chamber sometimes shape irregular

vitellogenic egg chamber late degenerated

larva number hatched none (< 10 %)

very few

adult lethality


abdominal odoriferous glands partially colour darker

larva lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality


larva lethality

larva lethality early (L5/6)

adult lethality

adult lethality

adult lethality


pupal molt delayed (still larva)

larva lethality early (L5/6)

head & thorax partially not present

posterior terminus potentially partially remaining

cuticle remnants without visible segmentation, differentiation or axis

legs partially remaining

cuticle remnants with some identifiable segments

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larval musculature pattern irregular

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

eclosion not fulfilled (with pupal and adult features)


adult lethality

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

adult lethality


larva lethality

larval musculature fluorescence decreased

affected eggs/embryos/larvae

adult lethality


eclosion delayed (still pupa)

pupal molt delayed (still larva)

antenna irregular

antenna can be shortened, malformed or missing

head partially not present

head irregular

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide


pupal molt delayed (still larva)

larva lethality

adult lethality


eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

adult lethality


pupal molt not fulfilled (died as prepupa)

eclosion not fulfilled (died as pupa)

legs orientation irregular

eclosion not fulfilled (died as pupa)

wings shortened

head orientation irregular

larva lethality

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


larva lethality

larva number hatched many (ca. 70-100 %)

pupa eclosion not fulfilled (died as pupa)


adult nutritional state potentially bad (cachectic)

adult nutritional state bad (cachectic)

ovary without detectable defects

egg production: number <50% of expected

adult lethality

scutellum irregular

flattened

hindwing folding irregular

elytra proximal median bent

wings not closed

wing veins pattern irregular

elytra slightly shape irregular

wings not closed


adult nutritional state bad (cachectic)

ovary without detectable defects

egg production: number <50% of expected

adult lethality

scutellum irregular

flattened

hindwing folding irregular

elytra proximal median bent

wings not closed

wing veins pattern irregular

elytra slightly shape irregular

wings not closed


eclosion delayed (still pupa)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality

head partially not present

abdomen partially not present

head shape irregular

head & thorax & abdomen partially not present

larval musculature partially potentially degenerated

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched

adult lethality


eclosion delayed (still pupa)

larva lethality


adult lethality

pupal molt delayed (still larva)


adult lethality

adult lethality

legs tarsomere sometimes distal cuticularisation decreased

labial palp thinner

maxillary palp partially not present

maxillary palp thinner

antenna distal bent

eclosion delayed (still pupa)

legs tarsomere partially not present

antenna distal colour lighter

adult appendages irregular

legs tarsus shape irregular

legs random not present

wings orientation irregular

maxillary palp elongated

labial palp elongated

labial palp thinner

maxillary palp thinner

female genital lobes mostly not present

wings not closed

antenna orientation irregular

pupal molt delayed (still larva)

larva lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality


larva lethality

adult lethality

adult nutritional state bad (cachectic)

egg production: number no/almost no eggs


larva lethality

adult lethality

adult lethality


fertility after larval injection decreased (no living offspring)

tropharium structure irregular

remaining cells seem to show no polyploidy (small nuclei)

egg chamber not present

tropharium size decreased

ovary size decreased

wings slightly orientation irregular

wings distal bent

wings not closed

egg chamber number increased

egg chamber potentially number decreased

vitellogenic egg chamber late not present

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

adult lethality

gula surface irregular

eclosion delayed (still pupa)

wings not closed

pupal molt delayed (still prepupa)

larva lethality

larva cuticle duplicated

many L1-L2 animals. First larval molt is potentially disrupted.

affected eggs/embryos/larvae

larval musculature fluorescence decreased

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number hatched none (< 10 %)

very, very few

adult lethality


head orientation irregular

wings not closed

oblique dorsal thoracic muscles median orientation irregular

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

adult nutritional state bad (cachectic)

adult lethality


pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still larva)

larva lethality

cuticle remnants strongly fragmented (cuticle crumbs)

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number hatched none (< 10 %)

adult lethality

pupa eclosion not fulfilled (died as pupa)

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


larva lethality

larva dorsal not closed

larva everted (inside-out)

larva complete everted (inside-out)

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched many (ca. 70-100 %)

adult lethality


eclosion delayed (still pupa)

larva lethality

head & thorax partially not present

labium not present

head & thorax & abdomen partially not present

affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

larval musculature pattern irregular

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched

adult lethality


fertility after larval injection decreased (no living offspring)

larva lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


larva lethality

adult lethality


eclosion delayed (still pupa)

female genital lobes with protrusion

female genital lobes size increased

female genital lobes shape irregular

pronotum shape irregular

pronotum slightly size decreased

legs orientation irregular

wings orientation irregular

antenna orientation irregular

eclosion not fulfilled (died as pupa)

abdominal segment 2 gin-traps anterior decreased

oblique dorsal thoracic muscles median shortened

elytra partially blistered

head orientation irregular

pupa bristle decreased

pupal molt delayed (still larva)

larva lethality

legs pretarsus distal duplicated

legs pretarsus not present

legs pretarsus shortened

prothorax potentially transformed

pygopodium segment potentially transformed

mesothoracic tracheal opening ectopically present

abdomen segment number increased

urogomphi potentially transformed

urogomphi not present

abdomen musculature number increased

The abdominal musculatur is increased by one segment. The urogomphy seem to be absent.

affected eggs/embryos/larvae

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg chamber late partially degenerated

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


flagellomeres number decreased

flagellomeres partially not present

ommatidia number decreased

eyes not present

antenna orientation irregular

larva lethality early (L5/6)

adult lethality

eclosion not fulfilled (with pupal and adult features)


metathoracic leg tarsus irregular

legs partially colour decreased

pupa potentially size decreased

larva lethality

vitellogenic egg chamber structure irregular

vitellogenic egg chamber number decreased

previtellogenic egg chamber not present

adult lethality

egg production: number no/almost no eggs

adult lethality

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

pupa eclosion not fulfilled (died as pupa)


abdominal odoriferous glands colour darker

ovary without detectable defects

mature egg deposition 2 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva lethality

larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

larva lethality early (L5/6)


adult lethality

adult lethality

larva lethality early (L5/6)

adult lethality


larva lethality early (L5/6)

vitellogenic egg chamber late not present

previtellogenic egg chamber not present

ovary size decreased

adult lethality


fertility after larval injection decreased (no living offspring)

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

larva appendages distal decreased

legs dorsoventral-axis irregular

larva ventral irregular

larva dorsal irregular

larva appendages shape irregular

larva dorsal not closed

abdomen segment number decreased

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larval musculature irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

abdomen decreased

larva dorsal irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


egg chamber decreased

mature egg decreased

egg production: number <50% of expected

larva lethality

Not detectable, animals lost.

adult lethality


larva lethality early (L5/6)


larva lethality early (L5/6)

abdomen segment decreased

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, no fluorescence

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


larva lethality early (L5/6)

adult lethality


larva lethality late (L7)

pupal molt not fulfilled (died with pupal and larval features)

pupal molt delayed (still larva)

larva lethality

adult lethality


ovary without detectable defects

ovariole decreased

larva lethality early (L5/6)

adult lethality

ovary without detectable defects

mature egg deposition 5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)

adult lethality

egg production: number <50% of expected


larva lethality early (L5/6)

larva number few (< 50 %) hatched


adult lethality

larva lethality early (L5/6)

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality


larva lethality early (L5/6)

larva posterior decreased

larval musculature posterior decreased

abdomen musculature posterior decreased

affected eggs/embryos/larvae


larva lethality early (L5/6)

abdomen & terminus dv-orientation irregular

larva dorsal not closed

larva appendages shape irregular

larva segmentation/division irregular

larva dorsal bent

larva bristle decreased

abdomen segment number decreased

Sometimes it is impossible to count abdominal segments.

head capsule decreased

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality immediately after eclosion

larva lethality early (L5/6)

ovary without detectable defects

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number no/almost no eggs

adult lethality

pupa eclosion not fulfilled (died as pupa)


adult lethality

larva lethality early (L5/6)

larva number few (< 50 %) hatched


larva lethality


larva lethality early (L5/6)

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

posterior terminus not present

prothorax potentially transformed

head appendages sometimes fused

legs shape irregular

legs number increased

metathorax sometimes partially not present

labium potentially transformed

labium position posteriorized

mandible sometimes not present

abdomen segment mostly number decreased

prothorax segment duplicated

antenna sometimes not present

larva segment number decreased

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

larva number hatched many (ca. 70-100 %)

adult lethality


fertility after larval injection decreased (no living offspring)

adult lethality

adult lethality


ovary without detectable defects

egg production: number <50% of expected

adult lethality

legs pretarsus irregular

Just one claw is present.

larva lethality early (L5/6)

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg not developed/not fertilized

embryo/egg with embryonic tissue, no cuticle, no fluorescence

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larva number few (< 50 %) hatched

adult lethality

larva segment partially not present

head & thorax partially not present


pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

eclosion not fulfilled (died as pupa)

larva lethality early (L5/6)

eclosion not fulfilled (with pupal and adult features)


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality early (L5/6)


hindwing irregular

elytra not closed

adult lethality

wings need term orientation irregular

wings need term not closed

wings potentially shortened

head not bent

larva lethality early (L5/6)

abdomen segment number increased

urogomphi potentially transformed

urogomphi not present

posterior terminus structure irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

ovary without detectable defects

adult lethality

adult nutritional state

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


larva lethality early (L5/6)

adult lethality


adult lethality

hindwing irregular

wings not closed

wings not closed

wings shortened

larva lethality early (L5/6)

affected eggs/embryos/larvae


pupal molt not fulfilled (died as prepupa)

head slightly orientation irregular

pupa size decreased

pupal molt delayed (still prepupa)

larva lethality early (L5/6)


adult lethality

larva lethality early (L5/6)

adult lethality


larva lethality late (L7)

larva lethality early (L5/6)


adult lethality

metathoracic leg pretarsus not present

metathoracic leg tarsus not present

metathoracic leg tibia not present

metathoracic leg partially distal necrotic

legs partially necrotic

Especially metathoracic legs are affected.

wings orientation irregular

larva lethality early (L5/6)

larval musculature fluorescence decreased

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


larva lethality

ovary without detectable defects

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality

pupa eclosion not fulfilled (died as pupa)


adult lethality

eclosion delayed (still pupa)

wings slightly not closed

pupal molt delayed (still larva)

larva lethality

labium not present

prothoracic leg sometimes present

maxilla not present

thorax & abdomen not present

Appendages shape/orientation sometimes irregular.

larval musculature irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality immediately after eclosion

larva lethality late (L7)

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

egg production: number <50% of expected


adult lethality

adult lethality

larva lethality early (L5/6)

adult lethality


adult lethality

larva lethality early (L5/6)

larva segment random not present

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


pupal molt delayed (still larva)

larva lethality late (L7)

larva lethality early (L5/6)

egg chamber mostly not present

ovary size decreased

adult lethality


larva lethality early (L5/6)

adult lethality

adult lethality


adult lethality

larva lethality early (L5/6)

There is an inscreasing number of damaged cuticles (probably from dead animals).

affected eggs/embryos/larvae

larval musculature random partially not present

affected eggs/embryos/larvae

larva number hatched many (ca. 70-100 %)


larva lethality early (L5/6)

egg production: number <50% of expected

adult lethality


wings not closed

elytra partially surface blistered

head irregular

labrum slightly orientation irregular

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


larva lethality early (L5/6)

larva number few (< 50 %) hatched

adult lethality


larva lethality early (L5/6)


adult lethality

adult lethality

larva lethality early (L5/6)

abdomen segment number decreased

head appendages random not present

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)


larva lethality early (L5/6)

larva anterior decreased

cuticle remnants without visible segmentation, differentiation or axis

affected eggs/embryos/larvae


fertility after larval injection decreased (no living offspring)

posterior terminus not present

labium not present

maxilla not present

abdomen segment number # of remaining segments  decreased

thorax not present

mandible size decreased

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


larva lethality early (L5/6)

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


larva lethality early (L5/6)

adult lethality

adult lethality

eclosion not fulfilled (with pupal and adult features)


eclosion not fulfilled (not completely eclosed)

adult lethality


larva lethality early (L5/6)

adult lethality

egg production: number <50% of expected

adult lethality


abdominal odoriferous glands content not present

adult lethality


adult lethality

larva lethality

abdomen irregular

abdomen decreased

larva lateral bent

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

embryo/egg not developed/not fertilized

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number few (< 50 %) hatched

adult lethality


adult lethality

larva lethality early (L5/6)

adult lethality

pupa eclosion not fulfilled (died as pupa)


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality early (L5/6)

egg chamber not present

Tropharia-lateral oviducts connection weakened.

tropharium structure irregular

ovary size decreased


elytra shortened

eclosion not fulfilled (died as pupa)

wings partially necrotic

wings orientation irregular

wings shortened

larva lethality early (L5/6)

ovary without detectable defects

adult lethality

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

adult lethality

adult lethality

adult lethality


larva lethality early (L5/6)

affected eggs/embryos/larvae

adult lethality


adult lethality

larva lethality early (L5/6)

adult lethality


eclosion not fulfilled (died as pupa)

wings slightly not closed

wings slightly shortened

legs orientation irregular

Especially T1 and T2 are affected.

adult lethality


pupal molt delayed (still larva)

larva lethality

larva posterior decreased

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


larva lethality early (L5/6)

adult lethality


adult lethality

adult lethality

larva lethality early (L5/6)

abdomen segment anterior number decreased

affected eggs/embryos/larvae

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched


adult lethality

wings not closed

wings slightly shortened

adult lethality


legs pretarsus fused

larva lethality early (L5/6)

adult lethality

adult nutritional state bad (cachectic)

egg production: number no/almost no eggs

adult lethality

adult lethality


adult lethality

larva lethality early (L5/6)

larva number few (< 50 %) hatched


adult lethality

larva lethality early (L5/6)


adult lethality

adult lethality

larva lethality early (L5/6)

labium not present

mandible not present

labrum not present

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

larva number few (< 50 %) hatched


adult lethality

larva lethality early (L5/6)

larva number hatched many (ca. 70-100 %)

adult lethality


larva lethality early (L5/6)

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


larva lethality


adult lethality

pupal molt delayed (still larva)

larva lethality

adult lethality

antenna not present

larva appendages swollen

legs podomer potentially fused

antenna shortened

larva appendages shortened

affected eggs/embryos/larvae

larval musculature potentially partially thinner

larval musculature fluorescence decreased


adult lethality

larva lethality early (L5/6)

posterior terminus not present

cuticle remnants with some identifiable segments

abdomen segment number decreased

head & thorax & abdomen partially not present

appendages sometimes remaining

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

very few

adult lethality

eclosion not fulfilled (with pupal and adult features)


fertility after larval injection decreased (no living offspring)

adult lethality

larva lethality early (L5/6)

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

larva lethality early (L5/6)

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

motility decreased

ovary without detectable defects

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

motility decreased

larva lethality early (L5/6)

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

larva lethality early (L5/6)

ovary without detectable defects

The number of eggs on day 13 is not much less compared to the other wells.

mature egg deposition 3 5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

adult nutritional state bad (cachectic)

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

adult lethality


tropharium decreased

egg chamber fused

larva lethality early (L5/6)

larva partially everted (inside-out)

larva complete everted (inside-out)

larval musculature pattern irregular

affected eggs/embryos/larvae

egg chamber sometimes number decreased

egg chamber partially degenerated

ovary without detectable defects

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality

adult lethality


larva lethality early (L5/6)

affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larval musculature pattern irregular

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality

adult lethality


eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality early (L5/6)

head & thorax partially not present

labium not present

head partially not present

larva partially constricted

adult lethality

pupa eclosion not fulfilled (died as pupa)


adult lethality

larva lethality early (L5/6)

adult lethality


adult lethality

larva lethality early (L5/6)

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva lethality late (L7)

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


eclosion not fulfilled (not completely eclosed)

larva lethality early (L5/6)

adult lethality


larva lethality early (L5/6)

affected eggs/embryos/larvae

The yolk in some of the eggs shows clearly more fluorescence than normal.

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


larva lethality early (L5/6)

tropharium sometimes structure irregular

mature egg not present

egg chamber number decreased

adult lethality

adult nutritional state potentially bad (cachectic)

egg production: number no/almost no eggs

adult lethality


adult lethality

larva lethality early (L5/6)

larva number hatched many (ca. 70-100 %)


adult lethality


larva lethality early (L5/6)

larval musculature partially degenerated

Potentially bad prep

affected eggs/embryos/larvae

adult lethality


larva lethality early (L5/6)

head & thorax partially not present

appendages sometimes remaining

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg with embryonic tissue, no cuticle, no fluorescence

embryo/egg not developed/not fertilized

larval musculature pattern irregular

larva number few (< 50 %) hatched

Larva collection well was leaky. Therefore difficult to score.

adult lethality


adult lethality

larva lethality early (L5/6)

cuticle remnants with some identifiable segments

head & thorax & abdomen partially not present

appendages random remaining

cuticle remnants without visible segmentation, differentiation or axis

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larval musculature pattern irregular

larva number hatched none (< 10 %)

eclosion not fulfilled (with pupal and adult features)


larva lethality early (L5/6)

head & thorax partially not present

cuticle remnants with unidentifyable segments

embryo/egg not developed/not fertilized

larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

about 50%

larva number few (< 50 %) hatched

very few

adult lethality


larva lethality early (L5/6)

larva appendages shape irregular

larva appendages shortened

adult lethality


pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

adult lethality

adult lethality


larva lethality early (L5/6)

adult lethality

pupa eclosion not fulfilled (died as pupa)


ovary without detectable defects

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality

adult lethality


larva lethality early (L5/6)

adult lethality


fertility after larval injection decreased (no living offspring)

eclosion not fulfilled (not completely eclosed)

wings partially swollen

wings partially blistered

wings slightly not closed

larva lethality early (L5/6)


eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality early (L5/6)


ovary without detectable defects

eclosion delayed (still pupa)

eclosion delayed (still pupa)

adult lethality

larva lethality early (L5/6)


larva lethality early (L5/6)

abdomen segment number decreased

legs podomer potentially fused

larva appendages shortened

larva number few (< 50 %) hatched

about 50%


adult lethality

adult lethality

larva lethality early (L5/6)

adult lethality immediately after eclosion


larva lethality

larva number hatched many (ca. 70-100 %)

adult lethality


larva lethality late (L7)

pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

larva lethality early (L5/6)

adult lethality

adult nutritional state bad (cachectic)

egg production: number no/almost no eggs

adult lethality

adult lethality


pupal molt not fulfilled (died with pupal and larval features)

tropharium slightly structure irregular

The tropharia look rougher. This was true for sthe stronger phenotypes.

egg chamber segmentation/division not present

egg chamber fused

mature egg deposition 3 >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


larva lethality early (L5/6)

cuticle remnants cuticle remnants with unidentifyable segments

abdomen partially not present

head & thorax & abdomen partially not present

abdomen is stronger affected than thorax and head

appendages partially remaining

cuticle remnants cuticle remnants without visible segmentation, differentiation or axis

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larval musculature pattern irregular

embryo/egg not developed/not fertilized

ovary without detectable defects

larva number few (< 50 %) hatched

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality early (L5/6)

cuticle remnants with some identifiable segments

appendages sometimes remaining

cuticle remnants without visible segmentation, differentiation or axis

larval musculature pattern irregular

embryo/egg not developed/not fertilized

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)


larva lethality late (L7)

larva lethality early (L5/6)

adult lethality

adult lethality

adult lethality

adult lethality


larva lethality late (L7)

larva lethality early (L5/6)

adult lethality

adult lethality

adult lethality


abdominal odoriferous glands content darker

adult lethality

adult lethality

abdomen partially not present

abdomen partially everted (inside-out)

larval musculature fluorescence decreased

larval musculature pattern irregular

larva number hatched many (ca. 70-100 %)

egg production: number <50% of expected

adult lethality


fertility after larval injection decreased (no living offspring)

egg production: number <50% of expected

antenna distal pigmentation/tanning lighter

larva lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


larva lethality early (L5/6)

abdomen segment number decreased

abdomen partially not present

head partially not present

appendages partially remaining

larval musculature pattern irregular

affected eggs/embryos/larvae


larva lethality early (L5/6)

gnathal appendages mostly not present

affected eggs/embryos/larvae

larval musculature partially not present

larval musculature pattern irregular

adult lethality


larva lethality early (L5/6)

adult lethality

egg production: number no/almost no eggs

adult lethality

adult lethality