adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

appendages partially remaining

cuticle remnants with some identifiable segments

embryo/egg not developed/not fertilized

larval musculature irregular

larva number hatched none (< 10 %)

adult lethality

adult lethality


adult lethality

previtellogenic egg chamber number decreased

vitellogenic egg chamber partially not present

egg chamber number decreased

mature egg not present

egg chamber position anteriorized

tropharium size shortened

vitellogenic egg chamber shape irregular

egg production: number no/almost no eggs

adult lethality

elytra dorsal not closed

eclosion not fulfilled (with pupal and adult features)

thorax segment sometimes not present

abdomen segment number decreased

legs segment shortened

head capsule size decreased

head & thorax & abdomen partially not present

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number hatched none (< 10 %)


adult lethality

adult lethality

larva number hatched many (ca. 70-100 %)


eclosion not fulfilled (died as pupa)

larva lethality

larva number hatched none (< 10 %)

adult lethality


eclosion not fulfilled (died as pupa)

adult lethality

larva lethality

egg shape rounded

egg size decreased

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

egg size decreased

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

egg size decreased

larva number hatched none (< 10 %)

egg production: number <50% of expected


adult lethality

adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

larval musculature irregular

abdomen segment number decreased

head & thorax & abdomen segment partially random not present

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality

adult lethality

elytra dorsal not closed

eclosion not fulfilled (died as pupa)

elytra surface irregular

head & thorax

Head and pronotum bent inwards towards the ventral side!!!

dorsal thoracic muscles pattern irregular

larva lethality

larva number hatched none (< 10 %)


adult lethality

elytra dorsal not closed

eclosion not fulfilled (died as pupa)

larva number few (< 50 %) hatched


adult lethality

adult lethality

adult lethality immediately after eclosion

eclosion not fulfilled (died as pupa)

larva lethality


adult lethality

eclosion delayed (still pupa)

pupal molt delayed (still larva)


adult lethality

ovary without detectable defects

adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected


larva lethality

larva lethality

larva number hatched none (< 10 %)

adult lethality

larva number hatched none (< 10 %)


ovary without detectable defects

egg production: number <50% of expected

No phenotype found!!!

eclosion not fulfilled (died as pupa)

larva lethality


larva lethality

larva number hatched none (< 10 %)

adult lethality


larva lethality

legs segment cuticularisation decreased

legs femur potentially fused

legs tibiotarsus potentially fused

legs tibiotarsus shortened

larval musculature mostly fluorescence not present

no fluorescence, because larvae are dead

larva number hatched none (< 10 %)


adult lethality

adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

larva number few (< 50 %) hatched


larva lethality

adult lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


adult lethality

adult lethality

eclosion not fulfilled (died as pupa)


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

larva number few (< 50 %) hatched


eclosion not fulfilled (died as pupa)

larva lethality

abdomen partially everted (inside-out)

larva number few (< 50 %) hatched


adult lethality

larva lethality

larva number hatched none (< 10 %)


pupal molt delayed (still larva)

larva lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


adult lethality

larva lethality

adult lethality


abdominal odoriferous glands sometimes size increased

ovary without detectable defects

egg production: number <50% of expected

No ovary phenotype!

larva lethality


eclosion not fulfilled (died as pupa)

larval musculature irregular


adult lethality

eclosion not fulfilled (died as pupa)

larval musculature fluorescence decreased

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected


adult lethality

egg chamber need term shape irregular

lateral oviduct complete not present

egg production: number <50% of expected

adult lethality

larva lethality

ovary without detectable defects

egg production: number <50% of expected

abdomen circumference decreased

larva partially everted (inside-out)

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

ovary without detectable defects

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

adult lethality

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

ovary without detectable defects

egg production: number <50% of expected

No phenotype found!!!!

adult lethality

eclosion not fulfilled (died as pupa)

larva cuticle duplicated

larva number few (< 50 %) hatched


eclosion not fulfilled (died as pupa)

larva lethality

larva number few (< 50 %) hatched


adult lethality

eclosion not fulfilled (not completely eclosed)

eclosion not fulfilled (died as pupa)

larva lethality

larva number few (< 50 %) hatched


larva lethality

adult lethality

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality

adult lethality


adult lethality

larva lethality

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larval musculature irregular

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)


adult lethality

larva number hatched many (ca. 70-100 %)


eclosion not fulfilled (died as pupa)

larva lethality

adult lethality

ovary without detectable defects

No phenotype!!!

adult lethality

head & thorax orientation irregular

Head and pronotum affected and curled inwards towards ventral surface of he adult. No effect observed during pupal stage.

elytra orientation irregular

larva lethality

legs segment shortened

legs sometimes fused

antenna sometimes not present

abdomen segment number decreased

thorax partially not present

head appendages orientation irregular

head & thorax & abdomen irregular

larval musculature irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

adult lethality

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult nutritional state bad (cachectic)

egg production: number <50% of expected

labrum size decreased

mandible potentially transformed

mandible not present

maxilla number increased

affected eggs/embryos/larvae

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality

larva lethality

head & thorax partially not present

head & thorax & abdomen partially not present

cuticle remnants with unidentifyable segments

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched

labrum size decreased

mandible potentially transformed

mandible not present

maxilla number increased

affected eggs/embryos/larvae

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality

adult lethality


eclosion not fulfilled (died as pupa)

elytra orientation irregular

antenna shape rounded

larva lethality

legs tibiotarsus shortened

legs pretarsus orientation irregular

abdomen musculature ventral potentially thinner

abdomen musculature dorsal potentially thinner

abdomen musculature lateral sometimes not present

one of the two oblique muscles sometimes in single abdominal segments misssing

abdomen musculature lateral orientation irregular

oblique lateral muscles not parellel to each other

larva number hatched none (< 10 %)


adult lethality

adult lethality

larva lethality

labrum size decreased

mandible potentially transformed

mandible not present

maxilla number increased

affected eggs/embryos/larvae

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

larva lethality

adult lethality


ovary without detectable defects

No phenotype found!

elytra size shortened

elytra dorsal not closed

antenna orientation bent

antenna orientation bent

elytra size shortened

larva lethality

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

adult lethality


adult lethality

ovary without detectable defects

No phenotype found!


adult lethality

eclosion not fulfilled (died as pupa)

adult lethality


adult lethality

larva lethality

cuticle remnants without visible segmentation, differentiation or axis

larva number hatched none (< 10 %)

adult lethality

adult lethality


larva lethality


elytra dorsal not closed

eclosion not fulfilled (died as pupa)

prothoracic leg partially not present

elytra size shortened

antenna orientation irregular

larva lethality

larva lethality late (L7)

larva lethality

adult lethality

adult lethality

larva number hatched none (< 10 %)


elytra dorsal not closed

eclosion not fulfilled (died as pupa)

prothoracic leg partially not present

elytra size shortened

antenna orientation irregular

larva lethality

adult lethality

adult lethality

larva number hatched none (< 10 %)


larva lethality

larva number hatched none (< 10 %)

adult lethality


larva lethality

larva number hatched none (< 10 %)


eclosion not fulfilled (died as pupa)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult nutritional state bad (cachectic)

larva number few (< 50 %) hatched

egg production: number <50% of expected


eclosion not fulfilled (died as pupa)

larva lethality


larva lethality

head & thorax cuticle dorsal irregular

head & thorax partially not present

larva number hatched none (< 10 %)


larva lethality

adult lethality

larva number hatched none (< 10 %)

adult lethality


pupal molt not fulfilled (died as prepupa)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still prepupa)

larva lethality

embryo/egg not developed/not fertilized

ovary without detectable defects

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (with pupal and adult features)

egg production: number no/almost no eggs


larva lethality

abdomen partially constricted

head partially remaining

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number few (< 50 %) hatched

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

embryo/egg not developed/not fertilized


eclosion not fulfilled (died as pupa)

wings not closed

embryo/egg not developed/not fertilized

egg shape rounded

previtellogenic egg chamber stage sequence irregular

vitellogenic egg chamber not present

mature egg not present

vitellarium shortened

mature egg deposition 3 >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


pupal molt not fulfilled (died as L7 or prepupa)

pupal molt not fulfilled (died with pupal and larval features)

pupal molt not completely molted

larva lethality

larva posterior not present

cuticle remnants

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larval musculature pattern irregular

larva number hatched none (< 10 %)


fertility after larval injection decreased (no living offspring)

adult lethality

larva lethality

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality

adult lethality


adult lethality

larva lethality

larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

eclosion not fulfilled (not completely eclosed)

eclosion not fulfilled (died as pupa)

wings shortened

wings size shortened

larva lethality

head appendages partially not present

prothorax segment irregular

prothorax appears to be shiftet towards anterior and fused with the head

legs partially sometimes bent

head capsule complete shape irregular


adult lethality

adult lethality

adult nutritional state bad (cachectic)

adult lethality

elytra not closed

adult lethality

posterior terminus segmentation/division irregular

urogomphi size decreased

legs random sometimes shape irregular

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


larva lethality

ovary without detectable defects

egg production: number <50% of expected

adult lethality


adult lethality

larva lethality

larva number hatched none (< 10 %)

embryo/egg not developed/not fertilized

adult lethality

adult lethality


eclosion delayed (still pupa)

pupal molt delayed (still prepupa)


adult lethality

larva lethality

larval musculature partially not present

adult lethality


larva lethality

head & thorax partially not present

cuticle remnants with unidentifyable segments

pupa eclosion not fulfilled (died as pupa)

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality

larval musculature irregular

larva number hatched none (< 10 %)

adult lethality

egg production: number <50% of expected

adult lethality

adult lethality


adult lethality immediately after eclosion

eclosion delayed (still pupa)

pupal molt delayed (still larva)


larva lethality late (L7)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality

larva cuticle cuticularisation decreased

labium distal structure irregular

maxillary palp structure irregular

legs tibiotarsus elongated

antenna elongated

larva appendages distal irregular

urogomphi mostly not present

legs pretarsus shape rounded

antenna antennal flagellum not present

larva bristle complete not present

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

larva number hatched none (< 10 %)

adult lethality


larva lethality

thorax musculature specific subset partially dorsal not present

thorax musculature specific subset dorsal partially rounded

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected


larva lethality

egg production: number no/almost no eggs

adult lethality


eclosion delayed (still pupa)

larva lethality


egg chamber mostly deposition

it seems that there is sometimes one egg-chamber depositioned in the tropharium

egg chamber mostly not present

tropharium shape rounded

tropharium size decreased

adult lethality

legs orientation irregular

wings size shortened

antenna size shortened

larva lethality

egg chamber stage sequence irregular

previtellogenic egg chamber number decreased

vitellogenic egg chamber mostly stage sequence not present

tropharium structure irregular

Tropharia seem to be filled with proocytes

tropharium shortened

larva number hatched none (< 10 %)

egg production: number <50% of expected


pupal molt not fulfilled (died as prepupa)

larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

ovary without detectable defects

egg production: number <50% of expected


larva lethality


eclosion delayed (still pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)


larva lethality late (L7)

younger than L7

pupal molt delayed (still larva)

larva lethality

adult nutritional state bad (cachectic)


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

adult lethality


larva lethality

abdomen musculature partially not present


fertility after larval injection decreased (no living offspring)

adult lethality

ovary without detectable defects

egg production: number <50% of expected

elytra structure irregular

eclosion not fulfilled (not completely eclosed)

elytra not closed

larva number hatched none (< 10 %)


larva lethality

legs trochanter not present

legs tibiotarsus not present

legs pretarsus not present

head & thorax & abdomen partially not present

abdomen partially constricted

larva appendages shortened

abdomen partially not present

legs segment distal not present

The upper joint of the trochanter, normally easily seen due to a strong fluorescence, could not be found.

larva appendages shape irregular

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

larval musculature irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence


adult lethality

larva lethality

eclosion not fulfilled (with pupal and adult features)


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

oblique dorsal thoracic muscles size increased

the inner oblique muscles are thicker

longitudinal dorsal thoracic muscles fluorescence decreased

larva lethality

abdomen musculature partially orientation irregular

abdomen musculature pattern irregular

larval musculature partially thinner

larval musculature partially not present

It's not sure whether larvae are still alive. Almost none of them hatched.

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number hatched none (< 10 %)


adult lethality

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae


larva lethality

adult lethality


adult lethality

procephalic head partially not present

procephalic head is either strangly malformed with the appengages barely visible or partial missing

head capsule size decreased

larval musculature irregular


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

egg production: number no/almost no eggs

adult lethality

adult lethality


adult lethality

legs pretarsus decreased

no pretarsal claws

eyes size decreased

antenna shortened

club fused

the 3 segments of the club seems to be fused

died with pupal and adult features

legs tibia thinner

funicle number decreased

oblique dorsal thoracic muscles size shortened

oblique dorsal thoracic muscles thinner

labial palp broader

labial palps distance broader

labrum size decreased

female genital lobes size decreased

head bristle mostly not present

abdomen gin-trap setae not present

antenna size shortened

abdomen gin-traps not present

eyes size decreased

tropharium structure irregular

egg chamber not present

tropharium rounded

tropharium shortened

vitellarium not present


mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult nutritional state bad (cachectic)

eclosion not fulfilled (died as pupa)

pupal molt not completely molted

larva lethality

larva number hatched none (< 10 %)

embryo/egg not developed/not fertilized

adult lethality

pupa eclosion not fulfilled (died as pupa)

adult lethality


abdominal odoriferous glands potentially size decreased

abdominal odoriferous glands colour darker

prothoracic odoriferous glands colour darker

larva lethality

larva number hatched many (ca. 70-100 %)

adult lethality

eclosion not fulfilled (died as pupa)


larva lethality

adult lethality

eclosion not fulfilled (died as pupa)


legs tarsus distal cuticularisation lighter

eclosion not fulfilled (not completely eclosed)

legs tarsus partially necrotic

larva lethality

ovary without detectable defects

egg production: number <50% of expected


pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still prepupa)

pupal molt not fulfilled (died as L7 or prepupa)

pupal molt not completely molted

pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

adult nutritional state bad (cachectic)

adult lethality


fertility after larval injection decreased (no living offspring)

adult lethality

abdomen posterior # of remaining segments  remaining

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number hatched none (< 10 %)

eclosion not fulfilled (with pupal and adult features)


ovary without detectable defects

egg production: number <50% of expected

gut partially remaining

head remaining

larva number few (< 50 %) hatched

larval musculature pattern irregular

embryo/egg not developed/not fertilized


adult lethality

larva lethality


adult lethality

eclosion delayed (still pupa)

eclosion delayed (still pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)


adult lethality immediately after eclosion

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

younger than L7 larva

pupal molt delayed (still larva)

younger than L7 larva

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

mature egg not present

vitellarium shortened

vitellogenic egg chamber mostly not present

ovary size decreased

egg production: number no/almost no eggs

adult lethality

mature egg not present

ovary without detectable defects

egg chamber mostly not present

adult nutritional state potentially bad (cachectic)

adult lethality


larva lethality

abdomen dorsal partially not closed

head & thorax & abdomen partially not present

cuticle remnants with unidentifyable segments

larval musculature pattern irregular

larva number few (< 50 %) hatched


adult lethality

larva lethality

larva number hatched many (ca. 70-100 %)


larva lethality

adult lethality


ovary without detectable defects

egg production: number <50% of expected


adult lethality

ovary size decreased

egg chamber mostly not present

only found one previtellogenic egg chamber in one ovariole

vitellarium size decreased

tropharium size decreased

larva lethality

abdomen partially dorsal not closed

larva partially everted (inside-out)

larval musculature pattern irregular

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched


larva lethality late (L7)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

gena triplet seta number increased

larval musculature thinner

ovary without detectable defects

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

pupa eclosion not fulfilled (died as pupa)


adult lethality

ovary without detectable defects

egg production: number <50% of expected

adult lethality

adult lethality

female genital lobes not present

eyes fluorescence present

we use crossings where only the female larvae show red fluorescence. because of the fact, that the pupa have no genital lobes, I checked the red fluorescence to be sure that they are females and no males


adult lethality


adult lethality

eclosion not fulfilled (not completely eclosed)

eclosion not fulfilled (died as pupa)

tropharium partially structure irregular

vitellarium potentially fused

vitellarium shortened

egg chamber complete not present

ovary surface rough

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


adult lethality

eclosion delayed (still pupa)

pupal molt delayed (still larva)

wings not closed

adult lethality

pupal molt delayed (still larva)

larva lethality

adult lethality


adult lethality

eclosion delayed (still pupa)

adult lethality

pupal molt delayed (still larva)

larva lethality


eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

head & thorax partially not present


adult lethality

ovary without detectable defects

egg production: number <50% of expected


embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

larva number hatched none (< 10 %)

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality

adult lethality


larva lethality

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)


eclosion not fulfilled (died as pupa)

pupal molt not fulfilled (died as prepupa)

adult lethality

pupal molt delayed (still prepupa)

wings size shortened

pupal molt not fulfilled (died with pupal and larval features)

larva lethality

adult nutritional state bad (cachectic)

egg production: number <50% of expected


larva lethality


fertility after larval injection decreased (no living offspring)

adult lethality

previtellogenic egg chamber broader

distance between egg chambers seems to be broader

previtellogenic egg chamber number increased

mature egg not present

vitellogenic egg chamber not present

egg production: number <50% of expected

larva lethality


pupal molt delayed (still prepupa)

larva lethality

adult nutritional state bad (cachectic)


eclosion delayed (still pupa)

adult lethality

pupal molt delayed (still larva)

larva lethality


larva lethality

adult lethality


larva lethality

eclosion delayed (still pupa)

adult lethality

pupal molt delayed (still larva)

larva lethality


mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva lethality


pupal molt not completely molted

died as larva or prepupa

pupal molt not fulfilled (died as L7 or prepupa)

larva lethality


larva lethality

pupal molt not completely molted

pupal molt delayed (still larva)


adult lethality

adult lethality

wings surface irregular

wings not closed

larva lethality

larva segment partially fused

appears like a segmentpolarity-phenotype

larva appendages shape irregular

Appendages are shorter and broader.

abdomen mostly not present

head partially anteroposterior-axis not present

larva cuticularisation decreased

head partially remaining

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larva number hatched none (< 10 %)

adult lethality


larva lethality

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality

larva number few (< 50 %) hatched


larva lethality

larva number hatched many (ca. 70-100 %)


died with pupal and adult features

larva lethality

larval musculature lateral slightly pattern irregular

larval musculature dorsal mostly shortened

larval musculature ventral sometimes shortened

larval musculature sometimes rounded

larval musculature ventral mostly not present

larval musculature dorsal partially not present

longitudinal ventral muscles are affected the most.irregularities of the lateral muscles possibly due to defect in the ventral and dorsal muscles.leg muscles normal

larva number hatched none (< 10 %)


adult lethality

pupal molt not fulfilled (died with pupal and larval features)

larva lethality


fertility after larval injection decreased (no living offspring)

ovary without detectable defects

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

elytra not closed

elytra shortened

wings shortened

larva lethality

cuticle remnants with unidentifyable segments

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

larval musculature irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number hatched none (< 10 %)

adult lethality

adult lethality immediately after eclosion


pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

larva lethality late (L7)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality late (L7)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

adult lethality

larva number hatched none (< 10 %)

adult lethality


egg chamber partially not present

tropharium partially shape irregular

tropharium partially present

vitellarium not present

abdomen partially everted (inside-out)

larva complete everted (inside-out)

larva cuticle dorsal not closed

abdomen complete everted (inside-out)

larva dorsal bent

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larval musculature irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larva number hatched none (< 10 %)

eclosion not fulfilled (with pupal and adult features)


adult lethality

adult lethality

died with pupal and adult features

adult lethality

eclosion not completely molted

all four beatles dead

oblique dorsal thoracic muscles partially rounded

larva lethality

larval musculature fluorescence not present

all larvae were dead

larva number hatched none (< 10 %)


adult lethality

larva lethality

larva number hatched many (ca. 70-100 %)

adult lethality


eclosion not fulfilled (not completely eclosed)

larva lethality

legs segment shortened

thorax partially not present

thorax & abdomen irregular

abdomen segment number # of remaining segments  decreased

in most cases 7 abdominal segments remaining, in some few cases less segments

thorax complete not present

larva constricted


larva lethality

larva number few (< 50 %) hatched

adult lethality


larva lethality


eclosion not completely molted

oblique dorsal thoracic muscles median shortened

oblique dorsal thoracic muscles median broader

muscle potentially split/not fused or ectopic muscle present

larva lethality


adult lethality

larva lethality

larva appendages rounded

legs segment potentially fused

larva appendages shortened

larval musculature irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

adult lethality


adult lethality

larva cuticle dorsal irregular

head & thorax & abdomen segment number decreased

cuticle remnants with unidentifyable segments

remnants inside-outed

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number few (< 50 %) hatched


pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still prepupa)

larva lethality

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality

adult lethality


wings anterior size shortened

eclosion not fulfilled (died as pupa)

wings partially necrotic

eclosion not fulfilled (died as pupa)

wings size decreased

larva lethality

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


adult lethality

larva lethality

larval musculature partially random not present

larvae dead --> muscle defect probably secundary effect

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

egg production: number <50% of expected

ovary without detectable defects

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


fertility after larval injection decreased (no living offspring)

larva lethality

larval musculature partially not present

larval musculature partially shortened

larval musculature partially rounded

unclear, whether phenotype is a real muscle phenotype or effect is secundary due to the death of the larvaeleg muscles not affected

larva number few (< 50 %) hatched


adult lethality

funicle number decreased

eclosion not fulfilled (not completely eclosed)

antenna shortened

eclosion not fulfilled (died as pupa)

antenna shortened

larva lethality

egg slightly shape rounded

exact percentage of eggs, which are smaller and rounded is hard to tell, because most of the empty eggs burst open during preparation

egg slightly size decreased

larva number hatched none (< 10 %)

adult lethality


larva lethality

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched

adult lethality


adult lethality

larva number hatched many (ca. 70-100 %)

adult lethality


pupal molt not fulfilled (died as L7 or prepupa)

longitudinal dorsal thoracic muscles fluorescence decreased

head orientation irregular

pupal molt delayed (still prepupa)

oblique dorsal thoracic muscles shortened

wings shortened

antenna orientation irregular

pupal molt delayed (still larva)

larva lethality

vitellogenic egg chamber number decreased

egg production: number <50% of expected

previtellogenic egg chamber mostly not present

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult lethality

wings not closed

larva lethality

abdomen partially everted (inside-out)

cuticle remnants with some identifiable segments

abdomen segment number decreased

head & thorax not present

gut remaining

cuticle remnants with unidentifyable segments

remnants inside-outed

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


larva lethality

larva lethality

larva lethality

labrum size decreased

mandible potentially transformed

mandible not present

maxilla number increased

affected eggs/embryos/larvae

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (died as pupa)


larva lethality

adult lethality


adult lethality

ovary without detectable defects

adult nutritional state potentially bad (cachectic)

motility decreased

adult lethality

elytra distal bent

elytra not closed

wings distal bent

head orientation irregular


adult lethality

egg production: number no/almost no eggs

adult lethality

adult nutritional state bad (cachectic)

motility decreased

adult lethality

elytra distal bent

elytra not closed

eclosion not fulfilled (died as pupa)

wings distal bent

head orientation irregular

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)

adult lethality

adult lethality


larva lethality


larva lethality

embryo/egg not developed/not fertilized

larval musculature irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence


fertility after larval injection decreased (no living offspring)

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

larva number few (< 50 %) hatched

adult lethality


larva lethality

larva number hatched many (ca. 70-100 %)


adult lethality

adult lethality

adult nutritional state bad (cachectic)

motility decreased

adult size decreased

adult lethality immediately after eclosion

head & thorax & abdomen dorsal not closed

head & thorax & abdomen partially not present

larva number few (< 50 %) hatched


adult lethality

larva lethality


adult lethality


adult lethality

adult lethality

larva lethality

larval musculature irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

larva number few (< 50 %) hatched

adult lethality

adult lethality


adult lethality

eclosion not fulfilled (not completely eclosed)

metathoracic leg orientation irregular

wings not closed

larva lethality

larva shape irregular

larval musculature mostly fluorescence not present

larval musculature thinner

larval musculature partially not present

larval musculature fluorescence decreased

larva number hatched none (< 10 %)


eclosion delayed (still pupa)

larva lethality early (L5/6)


larva lethality

abdominal odoriferous glands partially colour darker

egg production: number <50% of expected

ovary without detectable defects

larva number few (< 50 %) hatched

egg production: number <50% of expected


larva lethality

larva dorsal not closed

abdomen segment number decreased

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

larval musculature irregular

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched

adult lethality

adult lethality


larva lethality

larva anterior bent

T1 not missing, although it looks like that

larval musculature mostly rounded

larval musculature mostly not present

larval musculature complete fluorescence not present

dead larvae

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number hatched none (< 10 %)

eclosion not fulfilled (with pupal and adult features)


larva lethality

cuticle remnants with unidentifyable segments

cuticle remnants without visible segmentation but polarity

cuticle remnants were inside-outed

larval musculature irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number hatched none (< 10 %)


larva lethality

larva number few (< 50 %) hatched

larva number few (< 50 %) hatched

abdominal odoriferous glands size decrease


larva lethality

adult lethality

larva number hatched many (ca. 70-100 %)

adult lethality


egg production: number no/almost no eggs

adult nutritional state bad (cachectic)

died with pupal and adult features

eclosion not completely molted

all four beatles dead

eclosion not fulfilled (died as pupa)

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected


adult lethality

abdomen segment number decreased

thorax partially not present

larval musculature irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

larva number few (< 50 %) hatched

adult lethality

adult lethality


larva lethality

adult lethality


adult lethality

adult lethality

motility decreased

adult lethality

eclosion not fulfilled (died as pupa)

pupal molt not completely molted

larva lethality

thorax not present

head mostly not present

head & thorax & abdomen partially not present

abdomen partially upended (inside-outed)

egg shape rounded

larval musculature irregular

egg size decreased

larva number few (< 50 %) hatched

head appendages random not present

head & thorax cuticle dorsal irregular

head & thorax not present


larva lethality late (L7)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

adult lethality

gena triplet bristle number increased

head bristle pattern irregular

head capsule potentially size decreased

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


fertility after larval injection decreased (no living offspring)

motility decreased

adult lethality

ommatidia shape irregular

eclosion delayed (still pupa)

eyes size decreased

adult lethality immediately after eclosion

eclosion not completely molted

ommatidia number decreased

eyes fluorescence decreased

pupal molt not fulfilled (died as L7 or prepupa)


larva lethality


larva lethality

larva number few (< 50 %) hatched

adult lethality


larva lethality

abdomen sometimes partially everted (inside-out)

head & thorax & abdomen cuticle dorsal irregular

abdomen partially everted (inside-out)

gnathal appendages partially remaining

cuticle remnants with some identifiable segments

abdomen segment number decreased

head & thorax & abdomen segment partially not present

procephalic appendages partially remaining

cuticle remnants strongly fragmented (cuticle crumbs)

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larval musculature irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number few (< 50 %) hatched

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality

larva number hatched many (ca. 70-100 %)


died with pupal and adult features

larva lethality


adult lethality

larva lethality


adult lethality

cuticle remnants with unidentifyable segments

remnants inside-outed

larval musculature irregular

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected


eclosion delayed (still pupa)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality


pupal molt delayed (still larva)

pupal molt delayed (still larva)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

wings blistered

wings orientation irregular

pupal molt not completely molted

larva lethality early (L5/6)

mature egg number increased

potential held egg, but the division between the vitellogenic egg chambers does not seem to be normal.

egg chamber segmentation/division decreased

vitellarium shape irregular

adult nutritional state bad (cachectic)

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality early (L5/6)

eclosion not fulfilled (died as pupa)


larva lethality early (L5/6)

head & thorax partially not present

head & thorax & abdomen partially not present

head partially not present

head shape irregular

there are many different phenotypes on the slide

cuticle random remaining

cuticle remnants without visible segmentation, differentiation or axis

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

embryo/egg not developed/not fertilized

larval musculature pattern irregular

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

larva number few (< 50 %) hatched


adult lethality

larva lethality early (L5/6)

adult lethality


larva lethality early (L5/6)

larva partially not present

posterior terminus sometimes remaining

abdomen partially not present

abdomen partially everted (inside-out)

head sometimes partially remaining

embryo/egg without visible larval cuticle in cuticle preparation

larval musculature partially ectopically present

larval musculature partially not present

larval musculature pattern irregular

larva number few (< 50 %) hatched

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

adult lethality

adult lethality

adult lethality

eclosion not fulfilled (died as pupa)


larva lethality early (L5/6)

adult lethality

eclosion not fulfilled (died as pupa)


antenna orientation irregular

adult nutritional state

adult lethality

club distal size decreased

antenna orientation irregular

larva appendages shortened

urogomphi shortened

legs pretarsus shortened

larva appendages shape irregular


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality early (L5/6)

adult lethality

larva number few (< 50 %) hatched

about 50%?

adult lethality

eclosion not fulfilled (died as pupa)


abdominal odoriferous glands content darker

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

larva lethality early (L5/6)

larval musculature fluorescence decreased


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

adult lethality

adult lethality

adult lethality


adult lethality

larva segment partially not present

affected eggs/embryos/larvae


adult lethality

larva lethality early (L5/6)

larval musculature partially degenerated

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number few (< 50 %) hatched

adult lethality


adult lethality

wings slightly shortened

eclosion not fulfilled (not completely eclosed)

eclosion not fulfilled (died as pupa)

wings not closed

wings not closed

wings shortened

head not bent

egg chamber mostly not present

eclosion not fulfilled (with pupal and adult features)


adult lethality

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality early (L5/6)

larval musculature pattern irregular

affected eggs/embryos/larvae

mature egg deposition 5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

ovary without detectable defects

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number few (< 50 %) hatched

egg production: number

egg number questionable. eggs are potentially dessicated or smaller.

egg potentially size decreased

egg production: number

adult lethality


adult lethality

larva lethality early (L5/6)

adult lethality

labium not present

mandible not present

appendages sometimes partially remaining

gut sometimes remaining

abdomen segment number decreased

head partially not present

Mandibles and labium seem to be the most often affected head parts.

posterior terminus sometimes remaining

cuticle remnants with some identifiable segments

larva number few (< 50 %) hatched


eclosion delayed (still pupa)

pupa seta number decreased

abdomen gin-trap setae not present

pupa bristle number decreased

larva lethality early (L5/6)

vitellogenic egg chamber late partially degenerated

vitellogenic egg chamber potentially partially fused

tropharium potentially partially degenerated

tropharium slightly shortened

tropharium structure irregular

egg production: number no/almost no eggs

adult lethality

eclosion not fulfilled (died as pupa)


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

larva lethality early (L5/6)

larva cuticle thinner

most animals die potentially just before secreting a cuticle

embryo/egg without visible larval cuticle in cuticle preparation

larval musculature not present

Larval musculature development is either disrupted or the animals die before the muscles are formed.

larval musculature potentially degenerated

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number hatched none (< 10 %)

adult lethality


larva lethality early (L5/6)

larva number hatched many (ca. 70-100 %)

adult lethality


fertility after larval injection decreased (no living offspring)

labrum not present

only two larval cuticles were on the slide. both showed absence of the labrum. this might be the weak phenotype.

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (died as pupa)


adult lethality

eclosion not fulfilled (died as pupa)

oblique dorsal thoracic muscles thinner

oblique dorsal thoracic muscles not present

oblique dorsal thoracic muscles irregular

wings slightly shortened

larva lethality early (L5/6)

larval musculature partially not present

larval musculature partially thinner

larval musculature irregular

Penetrance: about 50%The muscle bundles seem to be thinner and more fragile than usual

vitellogenic egg chamber potentially partially degenerated

vitellogenic egg chamber structure irregular

vitellogenic egg chamber late not present

egg chamber number decreased

adult nutritional state potentially bad (cachectic)

larva number few (< 50 %) hatched

egg production: number <50% of expected

about 50%

adult lethality

eclosion not fulfilled (with pupal and adult features)


pupal molt not fulfilled (died as prepupa)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult nutritional state bad (cachectic)

egg production: number no/almost no eggs

adult lethality

adult lethality


egg chamber potentially number increased

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

eclosion delayed (still pupa)

larva lethality early (L5/6)

adult lethality


larva lethality early (L5/6)

adult lethality

ovary without detectable defects

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality

adult nutritional state bad (cachectic)

larva lethality

larva number hatched many (ca. 70-100 %)

adult lethality

abdomen segment number decreased

legs partially decreased

head & thorax & abdomen segment number decreased

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality late (L7)

female genital lobes not present

antenna size decreased

legs irregular

wings not present

gnathal appendages orientation irregular

pupa appendages irregular

pupal molt delayed (still larva)

larva lethality late (L7)

adult lethality

adult lethality


larva lethality early (L5/6)

adult lethality

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


larva lethality early (L5/6)

adult lethality

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


hindwing orientation irregular

elytra orientation irregular

larva lethality early (L5/6)


elytra not closed

larva lethality early (L5/6)

adult lethality


larva lethality

adult lethality


larva lethality early (L5/6)

adult lethality


larva lethality early (L5/6)

eclosion not fulfilled (died as pupa)


adult lethality

elytra posterior slightly colour decreased

larva lethality late (L7)

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg not developed/not fertilized

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

mature egg deposition 2 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


larva lethality early (L5/6)


adult lethality

adult lethality


larva lethality early (L5/6)

adult lethality

eclosion not fulfilled (died as pupa)


fertility after larval injection decreased (no living offspring)

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)


hindwing partially folding irregular

flagellomeres fused

elytra distal colour lighter

elytra partially blistered

elytra shortened

elytra surface irregular

flagellomeres number decreased

hindwing orientation irregular

elytra orientation irregular

wings orientation irregular

gnathal appendages partially orientation irregular

pupal molt delayed (still prepupa)

larva lethality early (L5/6)

adult lethality

adult nutritional state bad (cachectic)

adult lethality

eclosion not fulfilled (died as pupa)


eclosion delayed (still pupa)

larva lethality early (L5/6)

eclosion not fulfilled (died as pupa)

adult lethality


eclosion not fulfilled (not completely eclosed)

larva lethality early (L5/6)

mature egg deposition 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

mature egg deposition 2 >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

eclosion not fulfilled (with pupal and adult features)


eclosion delayed (still pupa)

larva lethality early (L5/6)

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality late (L7)

adult lethality

egg production: number no/almost no eggs

adult lethality

adult lethality


adult lethality

larva lethality early (L5/6)

larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

larva lethality early (L5/6)

adult lethality


larva lethality early (L5/6)

head partially not present

prothorax not present

head shape irregular

head & thorax partially not present

larval musculature potentially partially degenerated

larval musculature partially not present

larval musculature pattern irregular

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

larva number few (< 50 %) hatched


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

adult lethality

adult lethality

adult lethality

eclosion not fulfilled (died as pupa)


pupal molt not fulfilled (died as prepupa)

larva lethality late (L7)

pupal molt delayed (still prepupa)

larva lethality early (L5/6)


larva lethality early (L5/6)

posterior terminus not present

abdomen segment number decreased

thorax & abdomen partially not present

head partially remaining

cuticle remnants with some identifiable segments

usually at least the antenna is visible

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larval musculature pattern irregular

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

egg production: number <50% of expected

about 50%

adult lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected


eclosion not fulfilled (died as pupa)

larva lethality early (L5/6)

cuticle random remaining

cuticle remnants without visible segmentation, differentiation or axis

embryo/egg not developed/not fertilized

larval musculature pattern irregular

larva number hatched none (< 10 %)

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

adult lethality

adult lethality


larva lethality early (L5/6)

larval musculature pattern irregular

only very few animals, by far the most are not developed

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (died as pupa)

adult lethality

adult lethality


larva lethality early (L5/6)


larva lethality early (L5/6)

adult lethality

adult lethality

adult lethality


eclosion not fulfilled (died as pupa)

affected eggs/embryos/larvae

adult lethality

adult lethality

abdominal odoriferous glands structure irregular

abdominal odoriferous glands content not present

abdominal odoriferous glands partially content darker

prothoracic odoriferous glands colour darker

abdominal odoriferous glands colour darker


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

adult lethality

egg production: number no/almost no eggs

adult lethality

eclosion not fulfilled (with pupal and adult features)

eclosion not fulfilled (died as pupa)


tropharium size decreased

ovariole decreased

mature egg deposition 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

wings shortened

larva lethality early (L5/6)

larva number hatched many (ca. 70-100 %)


larva lethality early (L5/6)

adult lethality

adult nutritional state bad (cachectic)

adult lethality

adult lethality


pupal molt delayed (still larva)

pupal molt not fulfilled (died with pupal and larval features)

larva lethality early (L5/6)

antenna antennal flagellum shortened

larva bristle shortened

about 70%

adult lethality


adult lethality

wings not closed

wings distal orientation irregular

head & thorax & abdomen partially not present

cuticle remnants with unidentifyable segments

larva constricted

legs podomer potentially fused

legs shortened

larva segment not present

appendages random remaining

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larval musculature pattern irregular

larval musculature fluorescence decreased

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

about 50-60%

adult lethality


pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality

pupal molt not fulfilled (died as L7 or prepupa)

eclosion delayed (still pupa)

adult lethality


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

adult lethality


larva lethality

adult lethality


larva lethality


larva lethality

larva cuticle dorsal not closed

legs podomer shortened

head appendages shape irregular

abdomen segment number decreased

thorax partially not present

head appendages random not present

head & thorax & abdomen irregular

appendages random partially remaining

embryo/egg without visible larval cuticle in cuticle preparation

larval musculature irregular

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched

adult lethality


eclosion delayed (still pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality


eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality

thorax segment number decreased

head appendages orientation irregular

abdomen segment number decreased

legs podomer shortened

head appendages shape irregular

larva cuticle dorsal not closed

appendages partially random remaining

larval musculature irregular

adult lethality


eclosion delayed (still pupa)

larva lethality


ovary without detectable defects

only one ovary of four showed degraded vitellogenic egg chambers

adult lethality

eclosion delayed (still pupa)

larva lethality

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


eclosion not fulfilled (died as pupa)

pupal molt not completely molted

larva lethality

larva number hatched none (< 10 %)

adult lethality


larva lethality

abdomen musculature partially thinner

abdomen musculature sometimes rounded

abdomen musculature partially random not present

abdomen musculature pattern irregular

the leg musculature seems to be normal

larva number few (< 50 %) hatched


larva lethality

adult lethality

adult lethality


eclosion delayed (still pupa)

eclosion delayed (still pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality


adult lethality

eclosion delayed (still pupa)

eclosion delayed (still pupa)

eclosion delayed (still pupa)

larva lethality late (L7)

wings size shortened

pupal molt delayed (still larva)

larva lethality

egg chamber mostly not present

tropharium shape irregular

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality

appendages random remaining

larval musculature irregular

larva number few (< 50 %) hatched

adult lethality


abdominal odoriferous glands not present

adult lethality

larva lethality

larva number hatched many (ca. 70-100 %)


eclosion not fulfilled (died as pupa)

eclosion not fulfilled (died as pupa)

pupal molt not completely molted

larva lethality

labium shape irregular

maxilla shape irregular

antenna shape irregular

larva number few (< 50 %) hatched


fertility after larval injection decreased (no living offspring)

abdomen potentially constricted

abdomen circumference decreased

legs pretarsus shortened

legs pretarsus rounded

labium split

head appendages orientation irregular

larva cuticle dorsal not closed

abdomen segment number decreased

legs podomer shortened

head appendages shape irregular

larva circumference decreased

larval musculature irregular

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


larva lethality

larval musculature irregular


adult lethality

adult nutritional state bad (cachectic)

larva lethality

head appendages random not present

head capsule mostly not present

labrum size decreased

labrum potentially split

head & thorax & abdomen partially not present

abdomen partially not present

thorax partially not present

labrum not present

head & thorax partially not present


eclosion delayed (still pupa)

larva lethality

head appendages rounded

head appendages shortened

abdomen bristle pattern irregular

legs podomer shortened

head appendages shape irregular

head & thorax & abdomen irregular

larva number few (< 50 %) hatched


larva lethality

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

larval musculature irregular

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)


larva lethality


adult lethality

larva lethality

embryo/egg not developed/not fertilized

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number hatched none (< 10 %)


pupal molt delayed (still prepupa)

larva lethality

thorax segment partially not present

head appendages orientation irregular

head appendages shape irregular

head appendages random not present

head & thorax & abdomen irregular

abdomen segment partially not present

legs podomer shortened

head capsule size decreased

head & thorax irregular

adult lethality

adult lethality


adult lethality

egg production: number

adult lethality

adult lethality

eclosion not fulfilled (not completely eclosed)

eclosion not fulfilled (died as pupa)

larva lethality

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)


adult lethality

ovary without detectable defects

egg production: number <50% of expected

larva lethality


eclosion not fulfilled (died as pupa)

adult lethality

pupal molt delayed (still larva)

larva lethality

ovary without detectable defects

larva number few (< 50 %) hatched

egg production: number <50% of expected


eclosion delayed (still pupa)

larva lethality


adult lethality

larva lethality


adult lethality

larva lethality

adult lethality


adult lethality

larva lethality

embryo/egg not developed/not fertilized

larval musculature irregular

larva number hatched none (< 10 %)


pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still prepupa)

larva lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

adult lethality


adult lethality

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

larva lethality


pupal molt delayed (still larva)

pupal molt delayed (still larva)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

ovary without detectable defects

larva number hatched none (< 10 %)


pupal molt delayed (still larva)

pupal molt delayed (still larva)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

ovary without detectable defects

larva number hatched none (< 10 %)

adult lethality

adult lethality

adult lethality

adult lethality


eclosion delayed (still pupa)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

larva lethality

head & thorax partially not present

appendages random remaining

cuticle remnants with some identifiable segments

larval musculature irregular

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


larva lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


larva lethality

larva cuticle dorsal not closed

larva shape irregular

abdomen partially everted (inside-out)

larva complete everted (inside-out)

larval musculature irregular

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


adult lethality

adult lethality

adult lethality

larva lethality

larva number few (< 50 %) hatched

adult lethality


egg chamber stage sequence irregular

egg chamber partially degenerated

vitellogenic egg chamber partially not present

no late vitellogenic egg chambers

mature egg not present

tropharium anterior irregular

egg production: number <50% of expected

larva lethality

embryo/egg not developed/not fertilized

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


larva lethality


larva lethality

adult lethality


adult lethality

adult nutritional state bad (cachectic)

motility decreased

pupal molt delayed (still larva)

larva lethality

abdomen musculature partially not present

abdomen musculature lateral pattern irregular

leg musculature seems to be normal

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

larva number hatched none (< 10 %)


larva lethality

abdomen segment number decreased

thorax partially not present

head appendages random not present

head & thorax partially not present

larval musculature irregular

adult lethality

adult lethality


pupal molt not fulfilled (died as L7 or prepupa)

larva lethality late (L7)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality

egg chamber shape rounded

previtellogenic egg chamber slightly number decreased

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

adult lethality


larva lethality

larva number hatched many (ca. 70-100 %)

head mostly remaining

cuticle remnants strongly fragmented (cuticle crumbs)

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

head appendages random not present

abdomen segment number decreased

thorax partially not present

head capsule mostly not present

head & thorax & abdomen irregular

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

adult lethality

adult nutritional state bad (cachectic)

adult lethality


adult lethality


odoriferous glands size increased

abdominal odoriferous glands size increased

ovary structure without detectable defects

egg production: number <50% of expected

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


adult lethality

larva lethality

larva number hatched none (< 10 %)


adult lethality

pupal molt delayed (still prepupa)


larva lethality

adult lethality

adult lethality

adult lethality


adult lethality

adult lethality

larva lethality

gnathal appendages partially present

head mostly not present

head & thorax partially not present

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality

adult lethality


adult lethality

larva lethality

adult lethality


adult lethality

head & thorax & abdomen partially not present

cuticle remnants with some identifiable segments

larva number hatched none (< 10 %)

adult lethality


adult lethality

larva lethality


adult lethality

adult lethality

oblique dorsal thoracic muscles structure shortened

larva lethality

larva appendages shortened


pupal molt not fulfilled (died as prepupa)

embryo/egg not developed/not fertilized

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

egg size decreased

larva number hatched none (< 10 %)

adult lethality

vitellogenic egg chamber potentially degenerated

previtellogenic egg chamber not present

mature egg not present

egg chamber mostly not present


larva lethality


adult lethality

embryo/egg not developed/not fertilized

larval musculature irregular

larva number hatched none (< 10 %)


oblique dorsal thoracic muscles distal mostly structure shortened

longitudinal dorsal thoracic muscles mostly structure not present

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

antenna orientation bent

larva lethality

abdomen posterior partially not present

head & thorax & abdomen segment partially not present

cuticle everted (inside-out)

larval musculature irregular

larva cuticle partially dorsal not closed


eclosion not fulfilled (died as pupa)

larva lethality


larva lethality late (L7)

pupal molt delayed (still larva)

adult lethality

egg production: number <50% of expected

egg production: number no/almost no eggs

adult lethality

eclosion not fulfilled (with pupal and adult features)


pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

adult lethality

adult lethality

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)


larva lethality

larva number hatched many (ca. 70-100 %)


larva lethality

head capsule size decreased

head & thorax partially not present

prothorax partially not present

gnathal appendages mostly not present

larva everted (inside-out)

larval musculature irregular

larva number few (< 50 %) hatched

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

adult lethality


adult nutritional state

larva lethality

larva dorsal not closed

larva anterior not present

head mostly not present

larval musculature irregular

larva number few (< 50 %) hatched


adult lethality

ovary without detectable defects

larva lethality


larva lethality

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

adult lethality

adult lethality


larva lethality

larva cuticularisation decreased

egg shells look kind of wrinkled on one pole

larva number hatched none (< 10 %)


mature egg number increased

In each ovariole there is not more than one old follicle/mature egg, hence not held egg.

vitellogenic egg chamber number increased

antenna shape irregular

antenna cuticularisation decreased

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

ovary without detectable defects

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected


adult lethality

adult lethality


larva lethality

abdomen segment potentially partially fused

legs shape irregular

head & thorax & abdomen partially not present

adult lethality


larva lethality

motility decreased

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

abdomen mostly not present

posterior terminus shape irregular

abdomen partially not present

head capsule size decreased

head partially remaining


eclosion not fulfilled (died as pupa)

larva lethality

larva lethality

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

cuticle remnants without visible segmentation, differentiation or axis

adult lethality

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected


eclosion not fulfilled (died as pupa)

elytra ventral not closed

pronotum orientation bent

Transverse furrow present.

head size decreased

labrum shape irregular

labrum swollen

head orientation irregular

legs size shortened

antenna orientation irregular

larva lethality


adult lethality

larva lethality

adult lethality

adult lethality immediately after eclosion


adult lethality

larva lethality

adult lethality


adult lethality

larva lethality

ovary without detectable defects

egg production: number <50% of expected

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

adult lethality


adult lethality

larva lethality

adult lethality

eclosion not fulfilled (died as pupa)


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult nutritional state bad (cachectic)

adult lethality


adult lethality

ovary without detectable defects

egg production: number <50% of expected

adult lethality

larva lethality

adult lethality


larva lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


larva lethality

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality

eclosion not fulfilled (died as pupa)


larva lethality

adult lethality


adult lethality

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality


ovary without detectable defects

egg production: number <50% of expected

adult lethality

larva lethality

abdomen posterior not present

procephalic head partially remaining

cuticle remnants strongly fragmented (cuticle crumbs)

larval musculature irregular


larva lethality


abdominal odoriferous glands sometimes content not present

abdominal odoriferous glands partially structure not present

abdominal odoriferous glands colour not present

adult lethality

posterior terminus segmentation/division irregular

abdomen segment number decreased

pygopodium not present


adult lethality

abdomen segment number decreased

pygopodium not present


adult lethality

eclosion not fulfilled (died as pupa)

oblique dorsal thoracic muscles median sometimes structure duplicated

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

larva lethality

pygopodium size decreased

urogomphi size decreased

legs shape irregular

head bristle pattern irregular

Very few larvae also show missing head appendages and /or irregular shape/orientation.

adult lethality


adult lethality

larva lethality

adult lethality


wings shortened

abdomen gin-traps shortened

eclosion not fulfilled (died as pupa)

elytra partially necrotic

female genital lobes shortened

elytra ventral not closed

antenna orientation irregular

embryo/egg not developed/not fertilized

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality


antenna size shortened

larva lethality

larva cuticle ventral cuticularisation irregular

larva cuticle dorsal cuticularisation not closed

abdomen shortened

cuticle remnants with some identifiable segments

abdomen partially transformed

legs shortened

abdomen segment number decreased

mesothorax segment number increased

head size decreased

larva dorsal bent

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larval musculature irregular

larval musculature fluorescence decreased

larva number few (< 50 %) hatched


adult lethality

larva lethality

larva number hatched many (ca. 70-100 %)

adult lethality


eclosion not fulfilled (died as pupa)

antenna unilateral partially not present

antenna unilateral not present

antenna unilateral shape irregular

larva number few (< 50 %) hatched


larva lethality

adult lethality

adult lethality

adult lethality


ovary without detectable defects

egg production: number <50% of expected

larva lethality

head mostly not present

larva anterior not present

adult lethality

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

female genital lobes structure rounded

wings structure shortened

antenna orientation irregular

larva lethality


adult lethality

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

ovary without detectable defects

egg production: number <50% of expected

egg production: number <50% of expected


eclosion not fulfilled (died as pupa)

ommatidia number decreased

eyes shape irregular

dorsal thoracic muscles structure irregular

antenna orientation bent

larva lethality

egg production: number <50% of expected

egg production: number <50% of expected


eclosion not fulfilled (died as pupa)

ommatidia number decreased

eyes shape irregular

antenna orientation bent

larva lethality

egg production: number <50% of expected

egg production: number <50% of expected


elytra dorsal not closed

larva lethality

legs podomer shortened

head appendages random not present

head ventral bent

head & thorax & abdomen irregular

abdomen segment number decreased

thorax partially not present

head capsule mostly not present

larva cuticle partially darker


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

larval musculature irregular


adult lethality

No phenotype found!

pupal molt delayed (still larva)

embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg not developed/not fertilized

embryo/egg with embryonic tissue, no cuticle, no fluorescence


larva lethality

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


egg production: number <50% of expected

No phenotype found!

eclosion not fulfilled (died as pupa)

larva lethality


egg production: number <50% of expected

No phenotype!

larva lethality


larva lethality


adult lethality

adult lethality

larva lethality

head capsule size decreased

abdomen segment number decreased

thorax partially not present

head bent

head & thorax & abdomen irregular


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality


eclosion not fulfilled (died as pupa)

larva lethality

larval musculature irregular

larva number few (< 50 %) hatched

adult lethality


mature egg not present

tropharium shape irregular

egg chamber number decreased

eclosion not fulfilled (died as pupa)

larva lethality

head appendages shape irregular

abdomen segment number decreased

legs podomer shortened

head capsule size decreased

head & thorax & abdomen irregular

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality

adult lethality immediately after eclosion


eclosion not fulfilled (died as pupa)

larva lethality

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

legs podomer shortened

head appendages random not present

thorax partially not present

head capsule mostly not present

head & thorax partially not present

larva number few (< 50 %) hatched


adult lethality

adult lethality

larva lethality

larva number hatched none (< 10 %)


larva lethality

larval musculature irregular

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality


adult lethality

larva lethality

gut remaining

larva cuticle dorsal not closed

legs podomer potentially fused

legs podomer shortened

thorax segment number decreased

abdomen segment number decreased

head capsule size decreased

head & thorax & abdomen partially not present

cuticle everted (inside-out)

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg not developed/not fertilized

larval musculature irregular

larva number few (< 50 %) hatched

adult lethality

eclosion not fulfilled (with pupal and adult features)


eclosion not fulfilled (died as pupa)


adult lethality

ommatidia potentially fused

antenna orientation bent

eclosion delayed (still pupa)

eyes structure irregular

adult lethality

oblique dorsal thoracic muscles size shortened

ommatidia potentially structure fused

longitudinal dorsal thoracic muscles structure not present

antenna orientation irregular

larva lethality

head & thorax & abdomen irregular

legs duplicated

legs pretarsus split

legs pretarsus shortened

legs pretarsus rounded

legs podomer shortened

thorax segment number decreased

larval musculature irregular

larva number hatched none (< 10 %)


adult lethality

eclosion delayed (still pupa)

mandible structure broader

elytra dorsal not closed

adult lethality

antenna size shortened

labial palp proximal position increased

maxillary palp structure broader

larva lethality

legs podomer shortened

thorax partially not present

head appendages rounded

head appendages shortened

abdomen segment number decreased

thorax complete not present

labium not present

head & thorax & abdomen irregular

larval musculature irregular

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)


egg production: number no/almost no eggs

adult lethality

elytra dorsal not closed

labial palp potentially structure not fused

larva lethality

head appendages orientation irregular

head appendages shape irregular

labium not present

thorax & abdomen not present

larval musculature irregular

larva number hatched none (< 10 %)


egg production: number <50% of expected

No phenotype found!

adult lethality

adult lethality

larva lethality


adult lethality

larva lethality

adult lethality


larva lethality


larva lethality

head appendages random not present

thorax partially not present

legs podomer potentially fused

legs podomer shortened

abdomen segment potentially transformed

abdomen segment number decreased

legs number increased

head capsule size decreased

cuticle everted (inside-out)

cuticle remnants strongly fragmented (cuticle crumbs)

larval musculature pattern irregular

larva number hatched none (< 10 %)


egg production: number >150% of expected

No phenotype found!!

eclosion not fulfilled (died as pupa)

larva lethality

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

larval musculature irregular

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide


adult lethality

larva lethality

larva number hatched none (< 10 %)

adult lethality


elytra surface irregular

elytra colour decreased

larva lethality

legs segment shortened

head appendages random not present

larva cuticle dorsal not closed

head & thorax partially not present

abdomen segment number decreased

thorax segment number decreased

head capsule size decreased

head & thorax & abdomen segment number decreased

larval musculature irregular

larva number few (< 50 %) hatched


adult lethality

egg production: number <50% of expected

No phenotype found!

larva lethality

larva number hatched many (ca. 70-100 %)


adult lethality

larva constricted

larval musculature fluorescence decreased


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

legs podomer shortened

head appendages shape irregular

head appendages orientation irregular

larva cuticle dorsal not closed

abdomen segment number decreased

thorax segment number decreased

head capsule size decreased

larval musculature irregular

ovary without detectable defects

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality

larva lethality


adult lethality

egg production: number <50% of expected

No phenotype found!

larva lethality

head capsule size decreased

maxilla potentially fused

antenna potentially fused

antenna fused with the maxilla

antenna size decreased

thorax & abdomen partially everted (inside-out)

abdomen complete everted (inside-out)

appendages partially random remaining

cuticle remnants with some identifiable segments

abdomen partially everted (inside-out)

metathorax everted (inside-out)

head appendages shape irregular

larva complete everted (inside-out)

cuticle everted (inside-out)

cuticle remnants without visible segmentation, differentiation or axis

embryo/egg without visible larval cuticle in cuticle preparation

larval musculature irregular

larva number hatched none (< 10 %)


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality


adult lethality

larva lethality

larval musculature irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number hatched none (< 10 %)

egg production: number <50% of expected


eclosion not fulfilled (died as pupa)

larva lethality

larva number few (< 50 %) hatched

adult lethality


larva lethality

larva number few (< 50 %) hatched

adult lethality

eclosion not fulfilled (died as pupa)


larva lethality

larva number hatched none (< 10 %)

adult lethality


larva lethality

adult lethality

adult lethality

adult lethality


adult lethality

legs podomer shortened

larva cuticle dorsal not closed

abdomen segment number decreased

thorax segment number decreased

thorax & abdomen partially not present

appendages partially remaining

larval musculature irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

larva number few (< 50 %) hatched


eclosion not fulfilled (died as pupa)

larva lethality

abdomen partially everted (inside-out)

abdomen segment size decreased

head ventral bent

larva dorsal bent

larval musculature irregular

larva number hatched none (< 10 %)


adult lethality

larva lethality

legs podomer shortened

head appendages size decreased

head appendages random not present

head appendages orientation irregular

head & thorax & abdomen partially not present

abdomen segment number decreased

thorax partially not present

head capsule size decreased

head & thorax irregular

larval musculature irregular

larva number hatched none (< 10 %)

eclosion not fulfilled (with pupal and adult features)


adult lethality

adult lethality

larva lethality

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


larva lethality

legs podomer shortened

head appendages shape irregular

larva shape broader

larva number few (< 50 %) hatched


egg production: number <50% of expected

No phenotype found.

eclosion not fulfilled (died as pupa)

antenna orientation bent

antenna orientation irregular

larva lethality

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality


adult lethality

adult lethality

elytra surface irregular

elytra dorsal not closed

larva lethality

larval musculature irregular

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

adult lethality


adult lethality

elytra colour irregular

Elytra surface irregular.

elytra not closed

oblique dorsal thoracic muscles need term distal size shortened

larva lethality

larva complete everted (inside-out)


larva lethality


adult lethality

larva lethality

head appendages shape irregular

head appendages random not present

thorax partially not present

head capsule mostly not present

head & thorax partially not present


adult lethality

larva lethality


larva lethality

adult lethality


pupal molt not fulfilled (died as prepupa)

adult lethality


adult lethality


larva lethality

larva cuticle dorsal not closed

legs femur potentially fused

legs tibiotarsus potentially fused

legs podomer shortened

head appendages shape rounded

abdomen shortened

thorax partially not present

head appendages size decreased

head & thorax & abdomen irregular


adult lethality

adult lethality

elytra pigmentation/tanning decreased

eclosion delayed (still pupa)

eclosion not fulfilled (died as pupa)

elytra colour irregular

larva lethality


eclosion not fulfilled (died as pupa)

larva lethality

larva complete everted (inside-out)

larval musculature irregular

larva number hatched none (< 10 %)


vitellogenic egg chamber not present

mature egg not present

egg production: number no/almost no eggs

eclosion not fulfilled (died as pupa)

larva lethality

egg chamber not present

larva number hatched none (< 10 %)

adult lethality


adult lethality

egg production: number <50% of expected

eclosion not fulfilled (died as pupa)

larva lethality

adult lethality


eclosion not fulfilled (died as pupa)

antenna orientation bent

antenna orientation bent

head complete orientation bent


eclosion not fulfilled (died as pupa)

larva lethality

antenna bent

head appendages orientation irregular

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality


egg production: number <50% of expected

No phenotype found!!!

eclosion not fulfilled (died as pupa)

antenna orientation irregular

larva lethality


adult lethality

elytra dorsal not closed

larva lethality

legs podomer shortened

head appendages random not present

head appendages orientation irregular

head & thorax irregular

abdomen posterior circumference decreased

abdomen everted (inside-out)

thorax segment number decreased

head capsule mostly not present

larva complete everted (inside-out)

larval musculature irregular


adult lethality

adult lethality

eyes shape irregular

oblique dorsal thoracic muscles potentially structure irregular

ommatidia sometimes number decreased

larva lethality


adult lethality

larva lethality

adult lethality


larva lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality

adult lethality

larva lethality

adult lethality


elytra not closed

adult lethality

dorsal thoracic muscles structure thinner

larva lethality

larva number few (< 50 %) hatched


eclosion not fulfilled (died as pupa)

larva lethality

larva number hatched many (ca. 70-100 %)


eclosion not fulfilled (died as pupa)

larva lethality

adult lethality


egg production: number >150% of expected

No phenotype found!!!

adult lethality

larva lethality

adult lethality


larva lethality


eclosion not fulfilled (died as pupa)

larva lethality

adult lethality

adult lethality

adult lethality


adult lethality

larva lethality

head appendages random not present

abdomen segment number decreased

legs podomer shortened

head capsule mostly not present

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

larva number few (< 50 %) hatched

larval musculature irregular

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality

larva lethality

eclosion not fulfilled (died as pupa)

antenna orientation bent

larva lethality

adult lethality

head appendages random not present

abdomen segment number decreased

legs podomer shortened

head capsule mostly not present

larva number few (< 50 %) hatched

larval musculature irregular

adult lethality

adult lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larval musculature fluorescence decreased

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

adult lethality

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


eclosion not fulfilled (died as pupa)

antenna orientation bent

larva lethality

adult lethality

adult lethality

affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

larva lethality

embryo/egg not developed/not fertilized

adult lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

adult lethality


adult lethality

antenna orientation irregular

head capsule size decreased

legs segment slightly shortened

abdomen constricted

antenna not present

head appendages orientation irregular

abdomen segment number decreased

thorax segment number decreased

head split

larva number few (< 50 %) hatched

adult lethality


eclosion

larva lethality


eclosion not fulfilled (died as pupa)

larva lethality


larva lethality

adult lethality


larva lethality

egg production: number <50% of expected

No phenotype found!

ovary without detectable defects

adult lethality

larva lethality

adult lethality

adult lethality


adult lethality

abdominal odoriferous glands complete structure necrotic

ovary without detectable defects

egg production: number <50% of expected

No phenotype found!

adult lethality

larva lethality

abdomen partially remaining

gut remaining

gnathal appendages random remaining

antenna not present

abdomen partially everted (inside-out)

procephalic appendages random remaining

cuticle remnants with some identifiable segments

larval musculature partially random not present

larval musculature rounded

larvae showing decreased muscle fluorescence have also thinner muscles, and the oblique lateral muscles are also missing.

abdomen musculature specific subset lateral thinner

thorax musculature thinner

thorax musculature specific subset partially not present

the oblique thoracic musculature was nt present. remaining longitudinal thoracic musculature was thinner than normal. penetrance of this phenotype is not sure (not able to see thoracic muscles when larvae is lying on the dorsal side).

larval musculature thinner

abdomen musculature specific subset lateral orientation irregular

abdomen musculature specific subset lateral not present

wt larvae have two oblique lateral muscles. in larvae showing the phenotype one or both oblique muslces were missing. if remaining, these muscles showed a wrong orientation and were thinner than in wt larvae. leg musculature seems to be unaffected.


eclosion not fulfilled (died as pupa)

adult lethality

larva lethality

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality

embryo/egg not developed/not fertilized

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number hatched none (< 10 %)


larva lethality

larva number hatched none (< 10 %)

adult lethality


larva lethality

abdomen constricted

abdomen mostly not present

gnathal appendages random remaining

abdomen everted (inside-out)

thorax complete not present

head appendages random not present

head & thorax & abdomen partially not present

procephalic appendages sometimes remaining

larval musculature irregular

larva number hatched many (ca. 70-100 %)


larva lethality

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

tropharium potentially shape irregular

egg chamber potentially fused

vitellogenic egg chamber number decreased

previtellogenic egg chamber not present

larva number hatched none (< 10 %)

egg production: number no/almost no eggs


larva lethality

tropharium potentially shape irregular

egg chamber potentially fused

previtellogenic egg chamber not present

larva number hatched none (< 10 %)

larva number hatched none (< 10 %)


larva lethality

tropharium potentially shape irregular

egg chamber potentially fused

previtellogenic egg chamber not present

larva number hatched none (< 10 %)


previtellogenic egg chamber number decreased

eclosion not fulfilled (died as pupa)

adult lethality

larva lethality

only six larvae on the slide showing this phenotype

larval musculature irregular

larval musculature fluorescence decreased

larva number few (< 50 %) hatched


adult lethality

eclosion not fulfilled (died as pupa)

adult lethality immediately after eclosion

antenna orientation irregular

larva lethality


larva lethality


adult lethality

eclosion not fulfilled (died as pupa)

oblique dorsal thoracic muscles sometimes size shortened

larva lethality

larva number hatched none (< 10 %)


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

larva number hatched none (< 10 %)