adult lethality

larva lethality early (L5/6)


eclosion not fulfilled (with pupal and adult features)

mesothoracic leg tarsus shortened

legs tibia shortened

One or two tarsomere are missing.

metathoracic leg distal not present

Tibia, Tarsus, Praetarsus are missing.

pupal molt not fulfilled (died as pupa)

legs decreased

wings orientation irregular

metathoracic leg distal necrotic

metathoracic leg distal decreased

legs distal necrotic

T1 and T2

affected eggs/embryos/larvae

affected eggs/embryos/larvae

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality

not fulfilled (with pupal and adult features)

pupal molt delayed (still larva)

larva lethality early (L5/6)

eclosion delayed (still pupa)

larva lethality

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

larva number hatched many (ca. 70-100 %)


larva lethality early (L5/6)


larva lethality early (L5/6)

affected eggs/embryos/larvae

abdomen dorsoventral-axis shortened

larva partially everted (inside-out)

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larval musculature pattern irregular


larva lethality early (L5/6)

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larval musculature irregular

larva number few (< 50 %) hatched

adult lethality


adult lethality

larva lethality early (L5/6)

adult lethality


larva lethality late (L7)

adult lethality


larva lethality early (L5/6)

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality

pupa eclosion not fulfilled (died as pupa)


larva lethality early (L5/6)

affected eggs/embryos/larvae

labrum not present

abdomen musculature partially not present

affected eggs/embryos/larvae

adult lethality


adult lethality

larva lethality

cuticle remnants without visible segmentation, differentiation or axis

head appendages partially not present

head & thorax & abdomen partially not present

head partially remaining

cuticle remnants with some identifiable segments

not developed/not fertilized

About 50% affected, 50% wt after counting

larva number few (< 50 %) hatched


adult lethality

affected eggs/embryos/larvae

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched

adult lethality


adult lethality

larval musculature pattern irregular

legs partially remaining

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


pupal molt delayed (still larva)

pupal molt delayed (still larva)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

vitellogenic egg chamber late not present

egg production: number <50% of expected

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

pupal molt not fulfilled (died as prepupa)

larva lethality early (L5/6)

adult lethality

egg production: number no/almost no eggs

adult lethality

adult lethality


abdominal odoriferous glands colour darker

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

ovary without detectable defects

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality

larva lethality early (L5/6)

larva number hatched none (< 10 %)

egg production: number <50% of expected


adult lethality

larva lethality early (L5/6)

abdomen partially remaining

appendages partially remaining

larval musculature irregular

larva number few (< 50 %) hatched

adult lethality


eclosion not fulfilled (with pupal and adult features)

elytra not closed

elytra shortened

antenna bent

pupal molt delayed (still pupa)

wings shape irregular

wings shortened

larva lethality early (L5/6)

mature egg not present

adult lethality


pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality

larva number hatched none (< 10 %)

adult lethality

adult nutritional state bad (cachectic)

egg production: number <50% of expected

adult lethality


larva lethality early (L5/6)

vitellogenic egg chamber segmentation/division irregular

vitellogenic egg chamber late rounded

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality

larva lethality early (L5/6)

labrum not present

labrum size decreased

head shape irregular

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg not developed/not fertilized

larval musculature fluorescence decreased

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

larva number few (< 50 %) hatched

adult lethality


larva lethality early (L5/6)

cuticle remnants without visible segmentation but polarity

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larval musculature pattern irregular

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


hindwing irregular

elytra not closed

larva lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality

pupa eclosion not fulfilled (died as pupa)


pupal molt delayed (still larva)

larva lethality early (L5/6)

adult lethality

adult lethality

adult lethality


larva lethality early (L5/6)

larva number hatched many (ca. 70-100 %)

adult lethality

adult lethality


adult lethality immediately after eclosion

adult lethality


not fulfilled (died as L7 or prepupa)

pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

larva lethality

egg chamber not present

larva number hatched none (< 10 %)

adult lethality

adult lethality


adult lethality

wings not closed

wings slightly shortened

larva lethality early (L5/6)

affected eggs/embryos/larvae

vitellogenic egg chamber number increased

larva number hatched none (< 10 %)

egg production: number <50% of expected

Very few eggs

egg production: number <50% of expected


ovary without detectable defects

egg production: number <50% of expected

pupal molt delayed (still larva)

pupal molt delayed (still pupa)

larva lethality early (L5/6)

adult lethality


adult lethality

larva lethality early (L5/6)

adult lethality


larva lethality early (L5/6)

cuticle remnants with unidentifyable segments

Many very different phenotypes. Most of them known from the typical backround, no identifiable system behind the phenotypes (no common defects).

embryo/egg not developed/not fertilized

larval musculature differentiation incomplete

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larva number few (< 50 %) hatched

adult lethality


pupal molt not fulfilled (died as L7 or prepupa)

pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality

adult nutritional state bad (cachectic)

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (with pupal and adult features)


pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality early (L5/6)

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality

adult nutritional state bad (cachectic)

egg production: number <50% of expected

adult lethality


larva lethality early (L5/6)


pupal molt delayed (still larva)

pupal molt delayed (still larva)

adult lethality

adult lethality

adult lethality


larva lethality early (L5/6)

adult lethality


pupal molt delayed (still larva)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality early (L5/6)

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult nutritional state bad (cachectic)

egg production: number <50% of expected

adult lethality


adult lethality

pupal molt

pupal molt delayed (still prepupa)

elytra partially colour decreased

pupal molt delayed (still larva)

larva lethality early (L5/6)

affected eggs/embryos/larvae

labrum not present

larva number hatched none (< 10 %)

vitellogenic egg chamber late not present

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality

pupal molt

pupal molt delayed (still prepupa)

elytra partially colour decreased

pupal molt delayed (still larva)

larva lethality early (L5/6)

affected eggs/embryos/larvae

labrum not present

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality

pupal molt

pupal molt delayed (still prepupa)

elytra partially colour decreased

pupal molt delayed (still larva)

larva lethality early (L5/6)

affected eggs/embryos/larvae

labrum not present

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


pupal molt not fulfilled (died as pupa)

wings slightly shortened

larva lethality early (L5/6)

affected eggs/embryos/larvae

affected eggs/embryos/larvae

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

larva number hatched none (< 10 %)


larva lethality early (L5/6)

head shape irregular

cuticle remnants without visible segmentation, differentiation or axis

larval musculature pattern irregular

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched

adult lethality


larva lethality

affected eggs/embryos/larvae

posterior terminus not present

abdomen segment partially not present

larva dorsal not closed

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched

adult lethality


larva lethality early (L5/6)

affected eggs/embryos/larvae

abdomen partially remaining

cuticle remnants with some identifiable segments

embryo/egg with embryonic tissue, no cuticle, no fluorescence

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality

adult lethality


larva lethality early (L5/6)

adult lethality

pupa eclosion not fulfilled (died as pupa)


larva lethality early (L5/6)

larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

larva lethality early (L5/6)

abdomen segment partially not present

larva cuticle thinner

larva number few (< 50 %) hatched

adult lethality


pupal molt not fulfilled (died as pupa)

larva lethality late (L7)

adult lethality

adult nutritional state bad (cachectic)

adult lethality


larva lethality early (L5/6)

affected eggs/embryos/larvae

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


adult lethality

larva lethality early (L5/6)

adult lethality


larva lethality

adult lethality

adult lethality


adult lethality

adult lethality


ovary without detectable defects

egg production: number <50% of expected

eclosion not fulfilled (died as pupa)

ovary without detectable defects

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected


adult lethality

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality


larva lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality

labrum size decreased

mandible potentially transformed

mandible not present

maxilla number increased

affected eggs/embryos/larvae

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality

adult lethality


adult lethality

delayed (still pupa)

eclosion delayed (still pupa)

elytra pattern irregular

wings shortened

pupal molt delayed (still larva)

antenna orientation bent

larva lethality

adult lethality

adult lethality


larva lethality

ovary without detectable defects

egg production: number >150% of expected

adult lethality


egg production: number <50% of expected

adult lethality

larva lethality

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched

adult lethality


delayed (still pupa)

One pupa showed necrotic tissue.

eclosion not fulfilled (died as pupa)

larva lethality

pronotum colour darker

head colour darker

adult ventral colour darker

adult lethality

adult lethality

egg production: number <50% of expected


eclosion not fulfilled (died as pupa)

larva lethality

adult lethality


adult lethality

adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

ovary without detectable defects

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

adult lethality


adult lethality

adult lethality immediately after eclosion

eclosion delayed (still pupa)

larva lethality


adult lethality

larva lethality

adult lethality

egg production: number <50% of expected

adult lethality

adult lethality

adult lethality


adult lethality

larva lethality

thorax partially not present

head partially not present

head seems to be fused with thorax sometimes

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

adult lethality


adult lethality

eclosion delayed (still pupa)

larva lethality

ovary without detectable defects

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality


larva lethality

abdomen not present

abdomen everted (inside-out)

thorax partially not present

head partially not present

larva fragmented

head partially remaining

larval musculature irregular


adult lethality

Four animals were dissected. There were 14 held eggs in 1 case and 11 in the 2nd case!The next 2 animals showed wildtype ovaries!

larva lethality

abdomen mostly not present

metathorax not present

larval musculature pattern irregular

egg production: number <50% of expected

egg production: number <50% of expected


adult lethality

eclosion delayed (still pupa)

pupal molt delayed (still larva)

adult lethality


adult lethality

delayed (still larva)

pupal molt delayed (still larva)

larva lethality

vitellogenic egg chamber mostly not present

proocytes seem completely absent and number of follicle cells is increased

previtellogenic egg chamber complete not present

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

eclosion not fulfilled (with pupal and adult features)


larva lethality

adult lethality


larva lethality

larval musculature irregular

affected eggs/embryos/larvae

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

adult lethality


adult lethality

eclosion not fulfilled (died as prepupa)

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality


ovary without detectable defects

egg production: number <50% of expected

not fulfilled (with pupal and adult features)

larva lethality

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

egg shell robustness decreased

embryo/egg not developed/not fertilized

egg shell surface sticky

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected


eclosion not fulfilled (died as pupa)

larva lethality

legs structure irregular

taken into account the hatched larvae as well!


not fulfilled (died as pupa)

larva lethality

larva everted (inside-out)

head capsule mostly not present

head & thorax partially not present

larval musculature irregular

larva number hatched none (< 10 %)

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

mature egg sometimes size multiplied

previtellogenic egg chamber mostly not present

adult lethality

egg production: number no/almost no eggs

adult lethality

adult lethality

pupa eclosion not fulfilled (died as pupa)


adult lethality

eclosion delayed (still pupa)

larva lethality

abdomen segmentation/division irregular

head & thorax partially not present

larva number hatched many (ca. 70-100 %)

adult lethality


eclosion not fulfilled (died as pupa)

metathoracic leg tarsus structure irregular

elytra blistered

larva lethality

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected


adult lethality

elytra irregular

elytra structure blistered

larva lethality

embryo/egg not developed/not fertilized

cuticle remnants with some identifiable segments

larva number few (< 50 %) hatched

adult lethality


adult lethality

pronotum ectopically sometimes surface protruding

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

prothoracic leg sometimes structure blistered

female genital lobes protruding

larva lethality

antenna irregular

Anntennal sense organ is duplicated or multiplied

urogomphi not present

abdomen segment number increased

labium not fused

ovary without detectable defects

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

larva number hatched none (< 10 %)

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality

eclosion delayed (still pupa)

larva lethality

thorax & abdomen & terminus partially not present

thorax segment partially fused

head & thorax & abdomen mostly not present

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality


ovipositor size constricted

larva lethality

larval musculature mostly degenerated


adult lethality

adult lethality

eclosion delayed (still pupa)

larva lethality

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality


No phenotype found!

larva lethality

larval musculature irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

adult lethality


larva lethality

legs pretarsus not present

head appendages partially orientation irregular

legs shape irregular

head appendages shape irregular

larva appendages structure irregular


larva lethality

head segmentation/division irregular

way of processing the larvae made it not possible to look for more head defects in the hatched larvae


larva lethality

abdomen partially constricted

abdomen partially everted (inside-out)


larva lethality

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched


adult lethality

larva lethality

adult lethality

adult lethality


adult lethality

eclosion delayed (still pupa)

larva lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

larva lethality

vitellogenic egg chamber complete not present

previtellogenic egg chamber number increased

tropharium size decreased

adult lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality

egg production: number <50% of expected

adult lethality

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


larva lethality

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


adult lethality

larva lethality

larva number hatched many (ca. 70-100 %)


larva lethality

adult lethality

adult lethality


adult lethality immediately after eclosion

not fulfilled (died as pupa)

larva lethality

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

head & thorax partially not present

abdomen segmentation/division irregular

cuticle remnants with unidentifyable segments

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

head & thorax partially not present

abdomen segmentation/division irregular

adult lethality

ovary without detectable defects

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

head & thorax partially not present

abdomen segmentation/division irregular

adult lethality

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

head & thorax partially not present

abdomen segmentation/division irregular

adult lethality


larva lethality

adult lethality

pupa eclosion not fulfilled (died as pupa)


antenna bent

elytra necrotic

mandible necrotic

eclosion delayed (still pupa)

larva lethality

mature egg deposition 4 5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

previtellogenic egg chamber number decreased

mature egg not present

vitellogenic egg chamber mostly not present

ovary size decreased

adult lethality


ovary without detectable defects

adult lethality

egg production: number <50% of expected

larva lethality

abdomen partially not present

thorax anterior partially not present

head mostly not present

head & thorax & abdomen partially not present

abdomen partially remaining

cuticle remnants without visible segmentation, differentiation or axis

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

adult lethality

larva lethality

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult lethality

adult lethality

eclosion delayed (still pupa)

larva lethality

head capsule mostly not present

adult lethality


previtellogenic egg chamber sometimes not present

vitellogenic egg chamber complete structure not present

mature egg complete not present

egg production: number no/almost no eggs

adult lethality

larva lethality

not developed/not fertilized

larva number hatched none (< 10 %)


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

previtellogenic egg chamber mostly not present

vitellogenic egg chamber not present

adult lethality


larva lethality

larva number hatched many (ca. 70-100 %)


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

thorax dorsal segmentation/division irregular

legs shape irregular

legs pretarsus not present

antenna antennal flagellum not present

larval musculature pattern irregular

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

adult lethality

adult lethality


ovary without detectable defects

egg production: number <50% of expected

eclosion delayed (still pupa)

larva lethality

head & thorax partially not present

adult lethality


ovary without detectable defects

adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

mature egg deposition 5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva lethality early (L5/6)

legs femur partially fused

legs tibiotarsus partially fused

legs shape irregular

labium size decreased

larva appendages partially size irregular

leg musculature pattern irregular

legs partially swollen

legs partially shortened

abdomen posterior circumference decreased

legs podomer sometimes potentially fused

larva appendages partially shortened

adult lethality

eclosion not fulfilled (died as pupa)


adult lethality

larva lethality

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)


adult lethality immediately after eclosion

eclosion not fulfilled (died as pupa)

larva lethality

larva number few (< 50 %) hatched


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

egg production: number <50% of expected

adult lethality


larva lethality

embryo/egg not developed/not fertilized

adult lethality


adult lethality

oblique dorsal thoracic muscles size shortened

larva lethality

adult lethality


larva lethality

adult lethality


adult lethality

eclosion delayed (still pupa)

larva lethality

adult lethality


adult lethality

previtellogenic egg chamber sometimes pattern irregular

Number of somatic cells sometimes INCREASED.

vitellogenic egg chamber shape rounded

adult lethality

delayed (still pupa)

larva lethality

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg not developed/not fertilized

ovary without detectable defects

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

thorax & abdomen partially not present

metathorax and abdomen not present

head not present

head & thorax partially not present


larva lethality

ovary without detectable defects

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected


larva lethality

ovary without detectable defects

egg production: number <50% of expected

pupa eclosion not fulfilled (died as pupa)


larva lethality

head capsule partially split

thorax & abdomen partially not present

abdomen partially remaining

Sometimes with some identifyable mouthparts

larval musculature partially not present

larval musculature pattern irregular

adult lethality

eclosion not fulfilled (with pupal and adult features)

abdomen anterior not present

metathorax not present


adult lethality

eclosion delayed (still pupa)

larva lethality

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

with embryonic tissue, no cuticle, no fluorescence

embryo/egg not developed/not fertilized

larva number hatched many (ca. 70-100 %)


larva lethality late (L7)

pupal molt delayed (still larva)

larva number hatched none (< 10 %)

adult lethality

egg production: number no/almost no eggs

adult lethality


labium not present

eclosion not fulfilled (died as pupa)

labrum size decreased

female genital lobes not present

mandible size broader

The distance between the mandibles is increased!

legs tarsus necrotic

legs shape irregular

head orientation bent

antenna orientation irregular

elytra size decreased

maxillary palp elongated

Maxillary palps has 5 segments instead of three.

larva lethality

larva cuticularisation decreased

cuticle remnants without visible segmentation, differentiation or axis

larval musculature irregular

behaviour irregular

females crawl on the wall; even after put to the ground they hurry to the wall of the well and walk along the lower part of it.Picture: left Phenotype (femals at the wall; some males on the bottom); right & bottom WT


adult lethality

eclosion delayed (still pupa)

larva lethality


adult lethality

adult lethality

eclosion delayed (still pupa)

larva lethality


larva lethality

adult lethality

adult nutritional state bad (cachectic)

motility decreased

adult lethality


larva lethality


eclosion not fulfilled (died as pupa)

adult lethality

eclosion delayed (still pupa)

larva lethality


adult lethality

larva lethality

eclosion not fulfilled (died as pupa)

larva lethality

larval musculature dorsal partially shortened

larval musculature dorsal partially not present

adult lethality

abdominal odoriferous glands colour darker

adult lethality


eclosion not fulfilled (died as pupa)

delayed (still prepupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

adult lethality


adult lethality

eclosion delayed (still pupa)

larva lethality late (L7)

antenna orientation bent

elytra partially blistered

wings shortened

pupal molt delayed (still larva)

larva lethality

vitellarium thinner

egg chamber mostly not present

ovary size decreased


adult lethality

delayed (still larva)

pupal molt delayed (still larva)

larva lethality


adult lethality

larva lethality

adult lethality


adult lethality

adult lethality

larva lethality

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence


eclosion delayed (still pupa)

larva lethality


previtellogenic egg chamber number decreased

ovary without detectable defects

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


ovary without detectable defects

egg production: number <50% of expected

legs tibiotarsus fused

legs femur fused

posterior terminus shape shortened

legs segment shortened

larva complete everted (inside-out)

larval musculature mostly not present

larval musculature not present

larva number few (< 50 %) hatched

adult lethality


wings shortened

wings blistered

pupal molt not fulfilled (died as pupa)

larva lethality early (L5/6)

egg chamber number decreased

larva number hatched none (< 10 %)

egg production: number <50% of expected


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

larva number hatched none (< 10 %)

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality

pupal molt not fulfilled (died as L7 or prepupa)

larva lethality early (L5/6)

adult lethality

larva number hatched none (< 10 %)

adult lethality

egg production: number <50% of expected


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

adult lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

adult lethality

adult lethality


ovary without detectable defects

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected


adult lethality

motility decreased

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected


adult lethality

motility decreased

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected

ovary without detectable defects

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality

larva lethality early (L5/6)

previtellogenic egg chamber mostly not present

egg chamber sometimes fused

egg chamber potentially degenerated

egg chamber mostly shape rounded

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

pupa eclosion not fulfilled (died as pupa)


larva lethality early (L5/6)

eclosion not fulfilled (with pupal and adult features)


adult lethality

motility decreased

adult lethality

elytra colour lighter

mesothoracic leg cuticle partially colour lighter

eclosion not fulfilled (died as pupa)

eclosion not fulfilled (not completely eclosed)

wings not closed

oblique dorsal thoracic muscles thinner

wings not closed

ovary without detectable defects

larva number hatched many (ca. 70-100 %)

egg production: number <50% of expected


adult lethality

larva lethality early (L5/6)

larva number few (< 50 %) hatched


larva lethality late (L7)

larva lethality early (L5/6)

adult lethality

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality

adult lethality


adult nutritional state bad (cachectic)

larval musculature shape irregular

larval musculature fluorescence decreased

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

adult lethality


ovary without detectable defects

egg production: number <50% of expected

adult lethality

larva lethality early (L5/6)


adult lethality

adult lethality

motility decreased

adult lethality

larva lethality early (L5/6)

larva number few (< 50 %) hatched

adult lethality


ovary without detectable defects

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

ovary without detectable defects

egg production: number <50% of expected


ovary without detectable defects

egg production: number <50% of expected

larva lethality early (L5/6)

head appendages position irregular

abdomen segment number decreased

gnathal appendages partially remaining

thorax segment sometimes not present

head capsule mostly not present

head & thorax irregular

procephalic appendages partially remaining

cuticle remnants with some identifiable segments

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

larval musculature irregular

larva number few (< 50 %) hatched


eclosion delayed (still pupa)

eclosion not fulfilled (died as pupa)

wings not closed

wings shortened

antenna position irregular

larva lethality early (L5/6)

egg shell spotted

egg chamber mostly not present

tropharium irregular

embryo/egg not developed/not fertilized

mature egg present

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


larva lethality early (L5/6)

larva lethality


adult lethality

adult size decreased

adult lethality


larva lethality early (L5/6)

abdomen musculature partially not present

the musculature seems to be ok in the first and in the second abdominal segments.


ovary without detectable defects

egg production: number <50% of expected


adult lethality immediately after eclosion

larva lethality early (L5/6)

leg musculature coxal musculature partially present

leg musculature mostly not present


adult lethality

motility decreased

adult lethality

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

adult lethality

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

adult lethality


larva lethality late (L7)

pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

vitellogenic egg chamber potentially degenerated

vitellogenic egg chamber number decreased

previtellogenic egg chamber not present

adult lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

eclosion not fulfilled (with pupal and adult features)


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality

larva lethality early (L5/6)

appendages partially remaining

larval musculature irregular

larva number hatched none (< 10 %)


eclosion not fulfilled (died with pupal and larval features)

eclosion delayed (still pupa)

oblique dorsal thoracic muscles partially shortened

larva lethality early (L5/6)

larva number hatched many (ca. 70-100 %)


larva lethality early (L5/6)

pupa eclosion not fulfilled (died as pupa)


ovary without detectable defects

mature egg deposition 4 5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality


adult lethality

egg production: number <50% of expected

larva lethality early (L5/6)


larva lethality late (L7)

larva lethality early (L5/6)

adult lethality

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality

adult lethality


larva lethality early (L5/6)


pupal molt not fulfilled (died as prepupa)

larva lethality early (L5/6)

thorax segment sometimes not present

head capsule mostly not present

head & thorax & abdomen cuticle dorsal not closed

cuticle remnants with unidentifyable segments

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched


eclosion not fulfilled (died as pupa)

larva lethality late (L7)

wings orientation irregular

pupal molt delayed (still larva)

larva lethality early (L5/6)

adult lethality

adult nutritional state

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality


larva lethality early (L5/6)

adult lethality


mature egg not present

previtellogenic egg chamber not present

egg chamber degenerated

tropharium shape irregular

egg production: number <50% of expected

larva lethality late (L7)

larva lethality early (L5/6)

cuticle remnants with some identifiable segments

head appendages partially not present

head appendages position irregular

larva cuticle dorsal not closed

appendages partially remaining


legs orientation irregular

wings shortened

female genital lobes broader

wings not closed

antenna orientation irregular

head & thorax & abdomen segment size decreased

legs segment broader

legs segment shortened

head & thorax & abdomen cuticle dorsal irregular

larval musculature irregular

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

labrum size decreased

mandible potentially transformed

mandible not present

maxilla number increased

affected eggs/embryos/larvae

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality

thorax segment sometimes not present

head capsule mostly not present

cuticle remnants without visible segmentation but ap-polarity

head & thorax cuticle dorsal not closed

posterior terminus remaining

larval musculature irregular

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched


mature egg not present

previtellogenic egg chamber number decreased

motility decreased

egg shape rounded

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult nutritional state

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

eclosion not fulfilled (with pupal and adult features)


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


larva lethality early (L5/6)

larva number hatched many (ca. 70-100 %)

adult lethality


larva lethality late (L7)

pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality early (L5/6)

vitellarium size increased

vitellarium shape irregular

egg chamber potentially fused

egg chamber complete not present

larva number hatched none (< 10 %)

egg production: number <50% of expected


larva lethality early (L5/6)

adult lethality

adult lethality


larva lethality early (L5/6)

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

embryo/egg with embryonic tissue, no cuticle, no fluorescence

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality

pupa eclosion not fulfilled (died as pupa)


adult lethality

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number no/almost no eggs

motility decreased

adult lethality

eclosion not fulfilled (not completely eclosed)


larva lethality early (L5/6)

head & thorax & abdomen cuticle dorsal not closed

thorax segment sometimes number decreased

head capsule mostly not present

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larval musculature irregular


larva lethality early (L5/6)

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched


ovary without detectable defects

egg production: number <50% of expected

larva number few (< 50 %) hatched

adult lethality


adult lethality

larva lethality early (L5/6)


wings partially blistered

wings partially necrotic

pupal molt not fulfilled (died as pupa)

larva lethality early (L5/6)

egg chamber slightly number decreased

egg chamber shape irregular

mature egg not present

larva number few (< 50 %) hatched

egg production: number <50% of expected


eclosion not fulfilled (died as pupa)


antenna shortened

not fulfilled (with pupal and adult features)

at this timepoint still living

ommatidia shape irregular

black border around the eye is missing. surface of the eye appears plain. the GFP expression of the ommatidia points to a reduced number of ommatidia as well as a malformation of the ommatidia

eclosion not fulfilled (died as prepupa)

pupal molt delayed (still prepupa)

female genital lobes decreased

oblique dorsal thoracic muscles fragmented

wings shortened

antenna shortened

pupal molt not fulfilled (died as pupa)

adult nutritional state

larva number hatched none (< 10 %)


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

adult lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected


larva lethality early (L5/6)

leg musculature coxal musculature shape irregular

the leg musculature is present, only the shape of the coxal muscles is irregular

larval musculature shortened

larval musculature shape irregular

larval musculature partially rounded

larval musculature partially not present

Effects seem to be stronger in the head and the thoracic musculature

larva number few (< 50 %) hatched

adult lethality


eclosion not fulfilled (with pupal and adult features)

larva lethality early (L5/6)

larval musculature shortened

larval musculature shape irregular

larval musculature partially rounded

larva number few (< 50 %) hatched

adult lethality


larva lethality early (L5/6)

larva number hatched many (ca. 70-100 %)


larva lethality early (L5/6)

larva number few (< 50 %) hatched


ovary without detectable defects

egg production: number <50% of expected

head appendages partially not present

head appendages position irregular

cuticle remnants with some identifiable segments

cuticle remnants strongly fragmented (cuticle crumbs)

head capsule not present

legs partially remaining

cuticle remnants with unidentifyable segments


larva lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


larva lethality early (L5/6)


pupal molt not fulfilled (died with pupal and larval features)

larva lethality

egg chamber potentially fused

egg chamber shape irregular

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


larva lethality early (L5/6)

adult lethality


abdomen gin-traps irregular

larva lethality early (L5/6)

thorax cuticle dorsal not fused

head capsule mostly not present

head & thorax irregular


adult lethality

larva lethality early (L5/6)

legs segment shortened

legs pretarsus sometimes shape irregular

legs tibiotarsus fused

legs femur fused

larval musculature ventral number decreased

larval musculature shape irregular

larval musculature lateral number decreased


eclosion not fulfilled (died as pupa)

larva lethality late (L7)

pupal molt not fulfilled (died as pupa)

pupal molt delayed (still larva)

larva lethality early (L5/6)

ovary without detectable defects

ovary size decreased

egg chamber number decreased

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality

motility decreased

pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

ovary without detectable defects

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


larva lethality early (L5/6)

larva everted (inside-out)

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

labrum size decreased

mandible potentially transformed

mandible not present

maxilla number increased

affected eggs/embryos/larvae

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality


larva lethality early (L5/6)

adult lethality

egg production: number no/almost no eggs

adult lethality

adult lethality


larva lethality late (L7)

larva lethality late (L7)

pupal molt delayed (still larva)

eclosion not fulfilled (died as pupa)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality

pupa eclosion not fulfilled (died as pupa)


larva lethality early (L5/6)

abdomen shape irregular

legs shape irregular

head & thorax mostly not present

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

larval musculature pattern irregular

adult lethality


mature egg number increased

ovariole number decreased

abdominal odoriferous glands partially colour darker

tropharium size increased

adult lethality

oblique dorsal thoracic muscles number increased

eclosion not fulfilled (not completely eclosed)

eclosion not fulfilled (with pupal and adult features)

female genital lobes rounded

oblique dorsal thoracic muscles size broader

larva lethality early (L5/6)

abdomen partially everted (inside-out)

abdomen mostly not present

metathorax not present

head appendages partially not present

larva appendages shape irregular

embryo/egg not developed/not fertilized

with embryonic tissue, no cuticle, no fluorescence

larval musculature irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larva number hatched none (< 10 %)


adult lethality

adult lethality

elytra partially blistered

elytra not closed

larva lethality early (L5/6)

head & thorax partially not present

cuticle remnants strongly fragmented (cuticle crumbs)

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

larval musculature pattern irregular

larva number few (< 50 %) hatched

adult lethality


larva lethality early (L5/6)

larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

larva lethality early (L5/6)

procephalic head shape irregular

head cuticle partially protruding

head partially not present

labrum not present

ovary without detectable defects

egg production: number <50% of expected

adult lethality


adult lethality immediately after eclosion

abdomen bristle pattern irregular

thorax ventral segmentation/division irregular

head partially not present

larval musculature partially not present

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number few (< 50 %) hatched

adult lethality


larval musculature dorsal shortened

larval musculature partially ap-orientation not present

adult lethality


egg production: number no/almost no eggs

adult lethality

pupal molt not fulfilled (died as pupa)

wings not closed

wings shortened

pupal molt not fulfilled (died as pupa)

adult nutritional state bad (cachectic)

adult lethality


adult lethality

eclosion delayed (still pupa)

larva lethality early (L5/6)

adult lethality


larva lethality early (L5/6)

pupa eclosion not fulfilled (died as pupa)


ovary without detectable defects

egg production: number <50% of expected

adult lethality

larva lethality early (L5/6)

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg not developed/not fertilized

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected


vitellogenic egg chamber not present

previtellogenic egg chamber number decreased

vitellarium size decreased

tropharium shape irregular

tropharium size shortened

tropharium number decreased

egg production: number <50% of expected

larva lethality early (L5/6)

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality early (L5/6)

embryo/egg not developed/not fertilized

larval musculature irregular

larva number hatched none (< 10 %)


adult lethality

eclosion not fulfilled (with pupal and adult features)

larva lethality early (L5/6)

adult lethality


adult lethality


larva lethality early (L5/6)

adult lethality


larva lethality early (L5/6)

abdomen not present

thorax partially present

embryo/egg not developed/not fertilized

adult lethality


pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still prepupa)

larva lethality early (L5/6)

adult lethality

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality early (L5/6)

adult lethality


larva lethality late (L7)

larva lethality early (L5/6)

adult lethality

adult lethality


embryo/egg not developed/not fertilized

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larva number few (< 50 %) hatched

adult lethality


larva lethality early (L5/6)

thorax partially not present

labium not present

head & thorax partially not present

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched

adult lethality


embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


larva lethality early (L5/6)


ovary without detectable defects

egg production: number <50% of expected

adult lethality


adult lethality

elytra shape irregular

elytra shortened

eclosion not fulfilled (died as pupa)

wings not closed

wings orientation irregular

wings shortened

pupal molt not fulfilled (died as pupa)

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched

adult lethality

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality

wings shortened

antenna orientation irregular

larva number few (< 50 %) hatched

adult lethality

adult lethality


larva lethality early (L5/6)

legs tibiotarsus fused

legs femur fused

abdomen partially constricted

legs shortened

head appendages shortened

larva appendages shortened

with embryonic tissue, no cuticle, no fluorescence

larval musculature irregular

larval musculature fluorescence decreased

larva number few (< 50 %) hatched


larva lethality late (L7)

pupal molt delayed (still prepupa)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

tropharium shape rounded

tropharium size decreased

egg chamber not present

vitellarium not present

adult lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


larva lethality early (L5/6)

larva number hatched many (ca. 70-100 %)

adult lethality immediately after eclosion


adult lethality

ovary without detectable defects

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality


larva lethality late (L7)

larva lethality early (L5/6)

adult lethality

motility decreased

adult lethality


larva lethality early (L5/6)

adult lethality


larva lethality early (L5/6)

adult lethality

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

egg chamber not present

vitellarium shortened

ovary size decreased

vitellarium thinner

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


larva lethality early (L5/6)

elytra dorsal not closed

larva lethality

adult lethality

larva number hatched none (< 10 %)


adult lethality

pupal molt precocious

pupal molt delayed (still larva)


larva lethality early (L5/6)

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality

adult lethality


ovary without detectable defects

egg production: number <50% of expected

larva lethality early (L5/6)

ovary without detectable defects

adult lethality

pupal molt delayed (still prepupa)

larva lethality

ovary without detectable defects

adult lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

ovary without detectable defects

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected

legs shape irregular

labium not present

prothorax not present

mandible not present

head & thorax irregular

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

labrum size decreased

mandible potentially transformed

mandible not present

maxilla number increased

affected eggs/embryos/larvae

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality


larva lethality early (L5/6)

gena triplet seta number increased

labrum not present

larva appendages shortened

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

adult lethality


mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality

adult lethality

elytra partially blistered

elytra not closed

larval musculature pattern irregular

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

egg production: number no/almost no eggs

adult lethality


adult lethality

adult lethality

adult lethality


larva lethality early (L5/6)


adult lethality

elytra not closed

eclosion not fulfilled (not completely eclosed)

larva lethality early (L5/6)

affected eggs/embryos/larvae

legs shape irregular

legs tibiotarsus sometimes shortened

leg musculature tb1-ptar1 size decreased

leg musculature femural musculature partially not fused

muscles in the femur seem not to converge

adult lethality


tropharium size decreased

egg production: number <50% of expected

adult lethality

pupal molt not fulfilled (died as pupa)

larva lethality early (L5/6)

tropharium sometimes shape rounded

previtellogenic egg chamber number increased

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality


pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still prepupa)

tropharium partially structure fragmented

egg chamber not present

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality early (L5/6)

thorax fragmented

abdomen structure irregular

head & thorax partially not present

cuticle remnants with unidentifyable segments

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg not developed/not fertilized

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larva number few (< 50 %) hatched

adult lethality


adult lethality

larva number hatched many (ca. 70-100 %)

egg production: number <50% of expected

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality immediately after eclosion

adult lethality

adult lethality


adult lethality


larva lethality early (L5/6)

larva number few (< 50 %) hatched

adult lethality

labrum size decreased

mandible potentially transformed

mandible not present

maxilla number increased

affected eggs/embryos/larvae

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality


larva lethality early (L5/6)

adult lethality


larva lethality early (L5/6)

procephalic head irregular

head with protrusion

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larval musculature fluorescence decreased

adult lethality


larva lethality early (L5/6)

adult lethality

adult lethality


eclosion not fulfilled (died as pupa)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

wings partially blistered

pupal molt delayed (still larva)

wings shortened

egg chamber not present

vitellarium elongated

adult lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


abdomen cuticle dorsal not fused

larva size decreased

larva shape irregular

Larvae seem to lack an inner support structure, appearance is softened, a-p squished, saccate. Sometimes they appear shrunk.

larva cuticle cuticularisation decreased

cuticle remnants without visible segmentation, differentiation or axis

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larval musculature complete not present

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


ovary without detectable defects

egg production: number <50% of expected

eclosion not fulfilled (died as pupa)

head & thorax mostly not present

gnathal appendages not present

thorax partially not present

head & thorax & abdomen partially not present

embryo/egg not developed/not fertilized

larval musculature pattern irregular

larva number few (< 50 %) hatched

adult lethality


adult lethality

prothorax not present

gnathal appendages not present

head & thorax partially not present


larva lethality late (L7)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality

adult lethality

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality

adult lethality


larva lethality early (L5/6)

adult lethality

pupa eclosion not fulfilled (died as pupa)

adult lethality


larva lethality early (L5/6)

gnathal appendages partially not present

thorax partially not present

head partially not present

larval musculature irregular

adult lethality

adult lethality

adult lethality


pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still prepupa)

larva lethality early (L5/6)

tropharium shape irregular

egg chamber mostly not present

egg production: number <50% of expected

adult lethality


larva lethality early (L5/6)

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

egg shell colour darker

egg shell colour darker

larva number hatched none (< 10 %)

adult lethality


adult lethality

larva lethality early (L5/6)

adult lethality


ovary without detectable defects

egg production: number <50% of expected

larva lethality early (L5/6)

larva cuticle mostly dorsoventral-axis irregular

larva complete everted (inside-out)

cuticle remnants with some identifiable segments

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

eclosion not fulfilled (with pupal and adult features)


ovary without detectable defects

larva number hatched many (ca. 70-100 %)

egg production: number >150% of expected

adult lethality

adult lethality


egg chamber sometimes fused

mature egg not present

tropharium structure fragmented

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


ovary without detectable defects

egg production: number <50% of expected

larva lethality early (L5/6)

abdomen constricted

abdomen partially present

head & thorax mostly fragmented

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

adult lethality


egg chamber not present

tropharium shape rounded

tropharium size decreased

vitellarium not present

egg production: number <50% of expected

larva lethality early (L5/6)

adult lethality

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected


eclosion not fulfilled (died as pupa)

pupal molt delayed (still prepupa)

adult lethality

pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

larva lethality

head potentially orientation irregular

adult lethality

antenna orientation bent

eclosion not fulfilled (died as pupa)

antenna potentially orientation irregular

larva lethality

tropharium irregular

egg chamber mostly not present

mature egg not present

adult lethality

larvae alive, muscles were contracting, but larvae not hatched

larva number few (< 50 %) hatched


adult lethality

egg production: number <50% of expected

adult nutritional state bad (cachectic)

adult lethality

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number hatched none (< 10 %)

adult lethality

adult lethality


larva lethality

leg musculature coxal musculature present

leg musculature sometimes not present

larva number few (< 50 %) hatched


previtellogenic egg chamber not present

prooocyten absent

egg production: number <50% of expected

larva lethality

larva number few (< 50 %) hatched


adult lethality immediately after eclosion

eclosion delayed (still pupa)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)


eclosion not fulfilled (died as pupa)

larva lethality

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

larva lethality


eclosion not fulfilled (died as pupa)

larva lethality

larva number few (< 50 %) hatched

adult lethality


adult lethality

larva lethality


adult lethality

larva lethality

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)


pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality

adult nutritional state bad (cachectic)

adult lethality


larva number hatched none (< 10 %)

adult lethality

adult nutritional state bad (cachectic)

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

larva lethality

abdomen posterior circumference decreased

abdomen partially everted (inside-out)

cuticle remnants without visible segmentation, differentiation or axis

larval musculature irregular

larva number hatched none (< 10 %)

labium slightly size increased

larval musculature pattern irregular

larva number hatched none (< 10 %)

adult lethality


eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

larva lethality

larva number hatched none (< 10 %)

adult lethality

adult nutritional state bad (cachectic)

egg production: number <50% of expected

adult lethality

adult lethality

previtellogenic egg chamber position irregular

follicular epithelium potentially anterior amount decreased

vitellogenic egg chamber not present

previtellogenic egg chamber amount increased

mature egg not present

adult lethality


eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

larva lethality

larva number hatched none (< 10 %)

adult lethality

adult nutritional state bad (cachectic)

egg production: number <50% of expected

adult lethality


adult lethality

eclosion delayed (still pupa)

pupa eclosion not fulfilled (died as pupa)


pupal molt not fulfilled (died as prepupa)

eclosion not fulfilled (with pupal and adult features)

pupal molt delayed (still larva)

antenna bent

pupal molt delayed (still prepupa)

pupal molt delayed (still prepupa)

wings orientation irregular

antenna orientation bent

pupal molt delayed (still larva)

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

egg chamber mostly not present

embryo/egg not developed/not fertilized

mature egg not present

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected


pupal molt not fulfilled (died with pupal and larval features)

pupal molt delayed (still prepupa)

larva lethality

larva number hatched none (< 10 %)

vitellogenic egg chamber not present

mature egg degenerated

mature egg not present

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

labrum size decreased

mandible potentially transformed

mandible not present

maxilla number increased

affected eggs/embryos/larvae

adult lethality

larva number hatched many (ca. 70-100 %)

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality

larval musculature partially fluorescence not present

larval musculature fluorescence decreased

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number hatched none (< 10 %)


wings size decreased

larva lethality

larva number few (< 50 %) hatched

adult lethality


adult lethality

larva number few (< 50 %) hatched

egg production: number <50% of expected

abdominal odoriferous glands less secretion


adult lethality


adult lethality

adult lethality

ommatidia shape irregular

eclosion not fulfilled (died as pupa)

wings not closed

eyes size decreased

oblique dorsal thoracic muscles broader

ommatidia shape irregular

legs orientation irregular

eyes size decreased

eclosion not fulfilled (died as pupa)

oblique dorsal thoracic muscles size shortened

wings not closed

antenna potentially size shortened

larva lethality

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

ovary without detectable defects

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


adult lethality

larva number hatched none (< 10 %)

egg production: number no/almost no eggs


adult lethality

adult lethality

eclosion not fulfilled (died as pupa)

pupal molt not fulfilled (with pupal and adult features)

larva lethality late (L7)

pupal molt delayed (still larva)

thorax & abdomen everted (inside-out)


larva lethality

larva number few (< 50 %) hatched

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

pupal molt delayed (still prepupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

larva lethality

adult lethality


ovary without detectable defects

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

ovary present

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

legs segment potentially fused

thorax tibiotarsus shortened

legs femur shortened

legs tibiotarsus broader

legs femur broader

pupa eclosion not fulfilled (died as pupa)


larva lethality

larvae with reduced fluorescence were still moving, so the decreasing fluorescence isprobably not to the death of the larvae

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)


adult lethality

eclosion delayed (still pupa)

larva lethality


larva lethality

egg size decreased

egg shape rounded

embryo/egg not developed/not fertilized

egg size decreased

larva number hatched none (< 10 %)

eclosion not fulfilled (with pupal and adult features)


eclosion not fulfilled (died as pupa)

larva lethality

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg not developed/not fertilized

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality immediately after eclosion

larva lethality

larva number few (< 50 %) hatched


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

adult lethality

eclosion delayed (still pupa)

eclosion not fulfilled (died as pupa)

larva lethality

thorax segment sometimes partially not present

antenna not present

antenna shape irregular

head capsule size decreased

abdomen constricted

legs sometimes random not present

head not present

larva number hatched many (ca. 70-100 %)

adult lethality


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

eclosion not fulfilled (died as pupa)

larva lethality


adult lethality

ovary without detectable defects

egg production: number <50% of expected

adult lethality

dorsal thoracic muscles mostly not present

larva lethality

abdomen partially everted (inside-out)

abdomen segment number decreased

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larval musculature irregular

adult lethality


larva lethality

head & thorax & abdomen partially not present

embryo/egg not developed/not fertilized

larval musculature fluorescence decreased

Fluorescence strongly decreased, but larvae were still alive

larval musculature irregular

larva number hatched none (< 10 %)


larva lethality

larva lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality

adult lethality

adult lethality


larva lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality

adult lethality


eclosion not fulfilled (died as pupa)

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality


pupal molt not fulfilled (died as L7 or prepupa)

adult lethality

eclosion not fulfilled (not completely eclosed)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva number few (< 50 %) hatched


eclosion not fulfilled (died as pupa)

adult lethality

eclosion delayed (still pupa)

larva lethality


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality


adult lethality

larva lethality

adult lethality


larva lethality

Eggs appaer to have strange black holelike structures.

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)


larva lethality late (L7)

pupal molt

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

egg production: number no/almost no eggs

adult lethality

adult lethality


larva lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


larva lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality

adult lethality


adult lethality

adult nutritional state bad (cachectic)

antenna shortened

antenna shortened

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

egg production: number <50% of expected


larva lethality early (L5/6)

larva lethality

larva number few (< 50 %) hatched

adult lethality


larva lethality early (L5/6)

larva number few (< 50 %) hatched


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality

larva number hatched none (< 10 %)


abdominal odoriferous glands colour darker

adult lethality


larva lethality

cuticle remnants without visible segmentation, differentiation or axis

Almost all the empty eggs show 2 or 3 large holelike structures (black).

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


larva lethality

abdomen partially not present

abdomen everted (inside-out)

larval musculature irregular

embryo/egg not developed/not fertilized


adult lethality

ovary without detectable defects

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

larva lethality

larval musculature partially dorsal structure irregular

larval musculature partially dorsal not present

eclosion not fulfilled (with pupal and adult features)


oblique dorsal thoracic muscles shortened

dorsal thoracic muscles fluorescence decreased

wings shortened

pupal molt not fulfilled (died as prepupa)

larva lethality early (L5/6)

ovary without detectable defects

egg chamber colour lighter

adult lethality


larva lethality early (L5/6)

larval musculature partially not present

larva number hatched many (ca. 70-100 %)

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality


ovary without detectable defects

egg production: number <50% of expected

pupal molt not fulfilled (died with pupal and larval features)

pupal molt delayed (still larva)

larva lethality

abdomen cuticle anterior constricted

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide


larva lethality early (L5/6)

adult lethality


adult lethality

larva lethality early (L5/6)

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

egg production: number <50% of expected

adult lethality


larva lethality

adult lethality

adult lethality

pupa eclosion not fulfilled (died as pupa)


adult lethality

adult lethality

adult cuticularisation sticky

adult lethality immediately after eclosion

larva lethality early (L5/6)


adult lethality

head & thorax & abdomen partially not present

head & thorax partially not present

cuticle remnants

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larval musculature irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larva number few (< 50 %) hatched


adult lethality

larva lethality early (L5/6)


larva lethality

head appendages shape irregular

head appendages orientation irregular

procephalic head mostly not present

head capsule size decreased

larval musculature irregular

adult lethality


larva lethality

previtellogenic egg chamber mostly not present

vitellogenic egg chamber not present

vitellarium thinner

egg production: number no/almost no eggs

adult lethality


larva lethality

adult lethality

adult lethality


adult lethality

larva lethality

cuticle remnants with some identifiable segments

cuticle remnants strongly fragmented (cuticle crumbs)

embryo/egg not developed/not fertilized

larval musculature irregular

larva number few (< 50 %) hatched


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

ovary without detectable defects

adult nutritional state bad (cachectic)

adult lethality


adult lethality

adult lethality

adult nutritional state bad (cachectic)

adult lethality

eclosion not fulfilled (not completely eclosed)

oblique dorsal thoracic muscles partially rounded

in some cases both oblique muscles are rounded and in other cases only the outer or the inner oblique muscle is rounded

larva lethality early (L5/6)

legs shortened

abdomen segment number # of remaining segments  decreased

thorax cuticle dorsal structure irregular

labium partially not present

larval musculature irregular

larva number few (< 50 %) hatched

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality

head partially not present

Head & appendages appear to be missing on one side.

larval musculature irregular

larva number hatched many (ca. 70-100 %)


larva lethality

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

larva number hatched none (< 10 %)

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality

adult lethality

eclosion not fulfilled (died as pupa)

wings not closed

wings not closed

wings shortened

larva lethality early (L5/6)

larva surface darker

larvae seem to have an increased development, about 3/4 are already larval stage two and appear to be unable to mold

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number hatched none (< 10 %)


adult lethality

abdomen partially everted (inside-out)

cuticle remnants

head & thorax partially not present

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

larval musculature irregular

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality

pupal molt precocious

larva lethality

adult lethality

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

egg production: number <50% of expected

adult lethality

pupa eclosion not fulfilled (died as pupa)


larva lethality


larva lethality late (L7)

larva lethality early (L5/6)

adult lethality

adult lethality


adult lethality


adult lethality

adult lethality immediately after eclosion


adult lethality

thorax & abdomen partially not present

head & thorax partially not present

head & thorax & abdomen partially not present

abdomen partially remaining

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larval musculature irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

adult lethality


larva lethality

thorax & abdomen partially not present

abdomen partially not present

abdomen not present

thorax partially not present

larva appendages partially shortened

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

larval musculature irregular


adult lethality

pupal molt delayed (still larva)

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

larva partially everted (inside-out)

larva everted (inside-out)

larval musculature partially fluorescence decreased


larva lethality early (L5/6)

adult lethality


previtellogenic egg chamber number decreased

mature egg not present

tropharium size decreased

egg production: number no/almost no eggs

adult lethality

larva lethality early (L5/6)

head shape irregular

antenna sometimes not present

head size decreased

gena triplet gena triplet posterior not present

legs shape irregular

head appendages shape irregular

larval musculature pattern irregular

larval musculature partially not present

larval musculature fluorescence decreased

mature egg deposition 4 >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected


eclosion not fulfilled (with pupal and adult features)

pupal molt not fulfilled (died as prepupa)

larva lethality

previtellogenic egg chamber mostly not present

mature egg not present

ovary size decreased

adult lethality


adult lethality

ovary without detectable defects

mature egg deposition 2 >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

larva lethality

ovary without detectable defects

adult nutritional state bad (cachectic)

larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality early (L5/6)

eclosion not fulfilled (died as pupa)

larva lethality late (L7)

antenna orientation irregular

wings shortened

antenna shortened

pupal molt delayed (still larva)

larva lethality

mature egg complete not present

ovary size decreased


adult lethality

abdomen musculature partially random not present

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide


motility increased

they climb up the well very fast

legs shortened


larva lethality

motility decreased

egg production: number <50% of expected

adult lethality


larva lethality

mature egg not present

vitellarium shortened

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

pupa eclosion not fulfilled (died as pupa)


pupal molt delayed (still prepupa)

larva lethality

adult lethality immediately after eclosion


adult lethality

head & thorax & abdomen segment partially not present

cuticle remnants without visible segmentation, differentiation or axis

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

larval musculature irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larva number few (< 50 %) hatched


ovary without detectable defects

egg production: number <50% of expected

larva lethality early (L5/6)

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality


egg chamber partially anteriorized

mature egg not present

vitellogenic egg chamber partially degenerated

previtellogenic egg chamber partially fused

tropharium shape irregular

egg production: number <50% of expected

adult lethality

legs podomer potentially fused

legs podomer random shape irregular

larval musculature irregular

adult lethality


larva lethality early (L5/6)

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


eclosion not fulfilled (died as pupa)

antenna shortened

wings not closed

wings shortened

antenna orientation irregular

pupal molt not fulfilled (died as L7 or prepupa)

larva lethality

ovary without detectable defects

adult nutritional state bad (cachectic)

egg production: number no/almost no eggs

egg production: number <50% of expected


larva lethality

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (with pupal and adult features)


fertility after larval injection decreased (no living offspring)

adult lethality

ovary without detectable defects

adult lethality

eclosion not fulfilled (not completely eclosed)

eclosion not fulfilled (died as pupa)

larva lethality

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

egg production: number <50% of expected


eclosion not fulfilled (died as pupa)

larva lethality

larva number hatched many (ca. 70-100 %)


ovary without detectable defects

egg production: number <50% of expected

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg not developed/not fertilized

egg size decreased

mature egg deposition 4 >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected


larva lethality

leg musculature sometimes random not present

leg musculature partially random thinner

adult lethality


larva lethality early (L5/6)

adult lethality


larva lethality

adult lethality

pupa eclosion not fulfilled (died as pupa)


abdominal odoriferous glands partially colour darker

died with pupal and adult features


adult lethality

adult lethality

adult nutritional state bad (cachectic)

adult lethality

cuticle remnants with some identifiable segments

head & thorax & abdomen partially not present

cuticle remnants without visible segmentation, differentiation or axis

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

egg chamber complete not present

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality early (L5/6)

mature egg not present

previtellogenic egg chamber mostly not present

ovary size decreased

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality


eclosion not fulfilled (with pupal and adult features)

larva lethality early (L5/6)


pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still prepupa)

pupal molt not fulfilled (died as pupa)

larva lethality

larva mostly everted (inside-out)

head & thorax mostly not present

abdomen mostly remaining

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

larval musculature irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number hatched none (< 10 %)

adult lethality


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality

larva number hatched many (ca. 70-100 %)

adult lethality


pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

larva lethality

ovary without detectable defects

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult nutritional state

larva number hatched none (< 10 %)

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality

leg musculature sometimes not present

body wall musculature without detectable defect


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

thorax cuticle dorsal irregular

head appendages sometimes position irregular

abdomen segment number decreased

legs sometimes not present

head appendages sometimes random not present

head & thorax & abdomen cuticle dorsal not closed

embryo/egg not developed/not fertilized

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number few (< 50 %) hatched


adult lethality

adult lethality

adult nutritional state bad (cachectic)

larva lethality

egg chamber mostly position irregular

egg chamber mostly shape irregular

yolk potentially ectopically present

egg production: number <50% of expected

egg production: number <50% of expected


eclosion not fulfilled (not completely eclosed)

eclosion not fulfilled (died as pupa)

pupal molt not fulfilled (died with pupal and larval features)

head bent

pupal molt delayed (still larva)

larva lethality

abdominal odoriferous glands complete not present

abdominal odoriferous glands colour not present

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number few (< 50 %) hatched

egg production: number no/almost no eggs

egg production: number <50% of expected

adult lethality


larva lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality

adult lethality

larva number hatched none (< 10 %)

adult lethality

egg production: number <50% of expected

adult lethality


larva lethality


adult lethality

ovary without detectable defects

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality

adult lethality


ovary without detectable defects

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

larva segment size decreased

larva appendages shortened

abdomen shape irregular

cuticle remnants with unidentifyable segments

remnants inside-outed

abdomen partially everted (inside-out)

larva cuticle dorsal irregular

cuticle remnants strongly fragmented (cuticle crumbs)

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larval musculature irregular

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched


larva lethality

adult lethality

adult nutritional state

larva number hatched many (ca. 70-100 %)


pupal molt not fulfilled (died as prepupa)

pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

larva lethality

egg chamber mostly not present

larva number few (< 50 %) hatched

egg production: number <50% of expected


larva lethality

adult lethality


adult lethality

adult lethality

elytra not closed

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality

larva number few (< 50 %) hatched

egg production: number <50% of expected


eclosion not fulfilled (died as pupa)

wings blistered

pupal molt delayed (still larva)

larva lethality

mature egg complete not present

vitellogenic egg chamber mostly not present

previtellogenic egg chamber not present

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

adult lethality


adult lethality immediately after eclosion


eclosion not fulfilled (died as pupa)

pupal molt delayed (still prepupa)

larva lethality


larva lethality

embryo/egg not developed/not fertilized

adult lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

pupa eclosion not fulfilled (died as pupa)


eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality


adult lethality

adult lethality

adult lethality

eclosion not fulfilled (died as pupa)

wings not closed

pupal molt delayed (still prepupa)

larva lethality

embryo/egg with embryonic tissue, no cuticle, no fluorescence

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)


pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult nutritional state bad (cachectic)

adult lethality


adult lethality

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

larval musculature irregular


adult lethality

eclosion not fulfilled (died as pupa)

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

larva lethality

head & thorax & abdomen segment number decreased

larva cuticle dorsal irregular

leg musculature not present

muscles in the coxa are present


eclosion not fulfilled (died as pupa)

adult lethality

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality

abdomen posterior bent

posterior terminus size decreased

thorax & abdomen cuticle dorsal not closed

abdomen musculature partially not present

vitellogenic egg chamber slightly shape irregular

ovary size decreased

egg chamber number decreased

larva number few (< 50 %) hatched

egg production: number <50% of expected

eclosion not fulfilled (with pupal and adult features)


larva lethality

mature egg not present

egg chamber shape rounded

ovary without detectable defects

egg production: number <50% of expected

adult lethality


adult lethality

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality

larva number few (< 50 %) hatched


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

larva lethality

head appendages sometimes position irregular

head appendages sometimes shape irregular

larva cuticle dorsal irregular

abdomen number decreased

legs sometimes random not present

head appendages sometimes random not present

larva complete everted (inside-out)

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larval musculature irregular


eclosion not fulfilled (died as pupa)

adult lethality

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality

head & thorax & abdomen segment sometimes number decreased

abdomen constricted

abdomen segment number decreased

legs segment shape irregular

head & thorax & abdomen cuticle dorsal irregular

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg not developed/not fertilized

larval musculature irregular

larva number few (< 50 %) hatched

adult lethality


pupal molt delayed (still prepupa)

larva lethality

larva number few (< 50 %) hatched


adult lethality

eclosion not fulfilled (died as pupa)

adult lethality

pupal molt delayed (still prepupa)

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality

adult lethality

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality


larva lethality

larva number hatched many (ca. 70-100 %)


larva lethality


larva lethality

adult lethality


fertility after larval injection decreased (no living offspring)

head appendages size decreased

thorax segment sometimes not present

head appendages random not present

head & thorax cuticle dorsal irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larval musculature irregular

larval musculature shape irregular

larval musculature fluorescence decreased

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number hatched none (< 10 %)


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)


eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

larva lethality

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality


adult lethality

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality

adult lethality


larva lethality

larval musculature irregular

embryo/egg not developed/not fertilized


eclosion delayed (still pupa)

larva lethality


larva lethality

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched


larva lethality

abdomen segment number decreased

abdomen constricted

abdomen not present

abdomen shape irregular

metathorax not present

larval musculature irregular


adult lethality

pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still prepupa)

larva lethality

larva number few (< 50 %) hatched

adult lethality


larva lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality

egg production: number >150% of expected


adult lethality

eclosion delayed (still pupa)

adult lethality


eclosion not fulfilled (died as pupa)

ovary without detectable defects

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

egg production: number <50% of expected


ovariole mostly not present

just terminal filaments left

pupal molt delayed (still prepupa)

larva lethality

legs orientation irregular

antenna shortened

head & thorax & abdomen shape irregular

embryo/egg not developed/not fertilized

larval musculature irregular

larva number few (< 50 %) hatched


adult lethality

egg production: number <50% of expected

adult lethality

larva lethality

abdomen segment number decreased

cuticle remnants strongly fragmented (cuticle crumbs)

larval musculature irregular

ovary without detectable defects

eclosion not fulfilled (with pupal and adult features)


adult lethality

eclosion delayed (still pupa)

larva number few (< 50 %) hatched

adult lethality


larva lethality

adult lethality


larva lethality