eclosion not fulfilled (died as pupa)

larva lethality

larva number few (< 50 %) hatched

egg production: number <50% of expected


eclosion not fulfilled (died as pupa)

larva lethality

larva number hatched many (ca. 70-100 %)


eclosion not fulfilled (died as pupa)

larva lethality


larva lethality

head appendages slightly shape rounded

abdomen bristle pattern irregular

head bristle pattern irregular

legs coxa potentially shortened

legs tibiotarsus shortened

abdomen segment number decreased

in all cases only one or two abdominal segments are missing.

legs femur shortened

head capsule size decreased

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larval musculature partially not present

larval musculature irregular

larva number hatched none (< 10 %)

egg production: number <50% of expected


adult lethality

elytra surface irregular

elytra dorsal not closed

eclosion not fulfilled (died as pupa)

eclosion not fulfilled (with pupal and adult features)

head & thorax orientation ventralized

Head pronotum curled inwards.

larva lethality


adult lethality

egg chamber shape irregular

egg production: number <50% of expected

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

larva lethality late (L7)

pupal molt delayed (still larva)

dorsal thoracic muscles size shortened

larva lethality

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

adult lethality


egg production: number <50% of expected

No phenotype found!

ovary without detectable defects

adult lethality

larva lethality


adult lethality

larva lethality

adult lethality

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

larva cuticle dorsal not closed

thorax sometimes partially not present

abdomen constricted

head capsule size decreased

legs tibiotarsus potentially fused

legs femur potentially fused

legs femur shortened

abdomen segment number decreased

legs tibiotarsus shortened

head appendages shape irregular

head & thorax & abdomen partially not present

appendages partially remaining

cuticle remnants with some identifiable segments

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

larva number few (< 50 %) hatched

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

abdomen segment number decreased

larval musculature fluorescence decreased


larva lethality

abdomen partially everted (inside-out)

legs pretarsus rounded

antenna flagellum shortened

abdomen posterior circumference decreased

legs pretarsus shortened

head appendages shape rounded


egg chamber mostly number not present

tropharium size shortened

eclosion not fulfilled (died as pupa)

larva lethality


eclosion not fulfilled (died as pupa)

larva lethality

legs podomer potentially fused

legs podomer shortened

abdomen segment number decreased

legs tibiotarsus shortened

head appendages shortened


eclosion not fulfilled (died as pupa)

larva lethality

legs segment shortened

larva constricted

thorax partially not present

abdomen segment number decreased

thorax complete not present

thorax & abdomen partially not present

larval musculature irregular

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

adult lethality


eclosion not fulfilled (died as pupa)

adult lethality


larva lethality


eclosion not fulfilled (died as pupa)

elytra partially swollen

larva lethality


adult lethality immediately after eclosion

larva lethality

adult lethality


adult lethality

eclosion

larva lethality


adult lethality

oblique dorsal thoracic muscles median size shortened

longitudinal dorsal thoracic muscles shape irregular

larva lethality

adult lethality


larva lethality

embryo/egg not developed/not fertilized

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


eclosion not fulfilled (died as pupa)

larva lethality

larval musculature irregular

adult lethality


adult lethality

ovary without detectable defects

egg production: number <50% of expected

No phenotype found!

adult lethality

maxillary palp size elongated

antenna orientation bent

eclosion not fulfilled (died as pupa)

maxillary palp size elongated

antenna orientation irregular


larva lethality

larva cuticle dorsal not closed

larva circumference decreased

larva complete everted (inside-out)

legs segment sometimes broader

larva number few (< 50 %) hatched


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality

adult lethality

larva lethality

eclosion not fulfilled (died as pupa)

larva lethality


adult lethality

eclosion not fulfilled (died as pupa)

abdomen bent

head appendages shortened

abdomen bristle pattern irregular

legs podomer potentially fused

abdomen segment size decreased

legs podomer shortened

head capsule mostly not present

larva number hatched none (< 10 %)

larval musculature irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality


eclosion not fulfilled (died as pupa)


larva lethality

larval musculature shortened

larval musculature lateral partially not present

larval musculature ventral partially not present

larval musculature dorsal partially not present

effect seems to be strongest in the thoracic and first abdominal segments. leg musculature seems not to be ffected at all.

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number few (< 50 %) hatched


adult lethality

No phenotype found!

eclosion

larva lethality

larva number hatched many (ca. 70-100 %)

eclosion not fulfilled (with pupal and adult features)


adult lethality

larva lethality


adult lethality


head size shortened

wings shortened

legs orientation irregular

head orientation irregular

eclosion not fulfilled (died as pupa)

antenna orientation irregular

maxillary palp structure not present

elytra size shortened

elytra ventral not closed

head appendages pattern constricted

pupal molt delayed (still larva)

larva lethality

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

larva number hatched none (< 10 %)

adult lethality


larva lethality

larva cuticle dorsal not closed

larva everted (inside-out)

abdomen segment number decreased

thorax & abdomen partially not present

cuticle everted (inside-out)

cuticle remnants with unidentifyable segments

procephalic appendages partially remaining

abdomen complete not present

thorax segment number decreased

head everted (inside-out)

head & thorax & abdomen partially not present

gnathal appendages random remaining

cuticle remnants with some identifiable segments

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

larval musculature irregular

larva number hatched none (< 10 %)

adult lethality


larva lethality


egg production: number <50% of expected

No phenotype found!

eclosion not fulfilled (died as pupa)

larva lethality

adult lethality


ovary without detectable defects

egg production: number <50% of expected

No phenotpe found!

adult lethality

larva lethality

embryo/egg not developed/not fertilized

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


eclosion not fulfilled (died as pupa)

pupal molt not fulfilled (died with pupal and larval features)

elytra dorsal orientation irregular

antenna orientation irregular

elytra partially swollen

maxillary palp size elongated

larva lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


elytra dorsal not closed

elytra sometimes surface irregular

eclosion not fulfilled (died as pupa)

elytra sometimes colour decreased

adult lethality

leg musculature not present

body wall muscle pattern ok. coxa musculature present.

larval musculature degenerated

larva number hatched none (< 10 %)


larva lethality

larval musculature irregular

larval musculature fluorescence decreased

larva number few (< 50 %) hatched


adult lethality

tropharium shape irregular

egg production: number no/almost no eggs

eclosion not fulfilled (died as pupa)

larva lethality

labium proximal shortened


eclosion delayed (still pupa)


adult lethality

adult lethality

larva lethality

adult lethality


legs shortened

legs orientation irregular

eclosion not fulfilled (died as pupa)

female genital lobes structure rounded

antenna shape irregular

maxillary palp

elytra size shortened

head orientation irregular

larva lethality

legs segment shortened

thorax mostly fragmented

abdomen complete fragmented

thorax complete fragmented

head appendages slightly shape irregular

thorax & abdomen fragmented

The fragmented thorax and abdomen look sometimes like they are inside-outed.

larval musculature irregular

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality

larva lethality


adult lethality

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva lethality

abdomen partially constricted

abdomen partially circumference constricted

larva random partially constricted

larval musculature irregular

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

larva number hatched none (< 10 %)

adult lethality


eclosion delayed (still pupa)

larva lethality

adult lethality


pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still larva)

larva lethality

mesothoracic tracheal opening not present

abdomen tracheal opening not present

leg musculature not present

head musculature not present

larva dorsal cuticularisation irregular

posterior terminus not present

larva bristle irregular

larva appendages size shortened

legs pretarsus sometimes duplicated

legs segmentation/division irregular

abdomen segment anteroposterior-axis decreased

legs size decreased

head capsule not present

larva appendages shape irregular

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

abdomen musculature ventral mostly not present

abdomen musculature specific subset lateral not present

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality

larva number hatched many (ca. 70-100 %)

adult lethality


eclosion not fulfilled (died as pupa)

adult lethality

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

elytra not closed

legs broader

Specifically the femur, tibiotarsus and pretarsus are affected.

legs shape irregular

affected eggs/embryos/larvae

larval musculature irregular

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched

egg production: number <50% of expected

legs pretarsus shortened

legs shortened

Tibiotarsus & Femur potentially fused.


fertility after larval injection decreased (no living offspring)

ovary without detectable defects

egg production: number <50% of expected

larva lethality


pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

larva lethality

adult lethality

adult lethality


mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

adult lethality

legs sometimes not present

maxillary palp thinner

labial palp elongated

maxillary palp elongated

elytra not closed

club cuticularisation not present

female genital lobes rounded

labial palp elongated

maxillary palp thinner

female genital lobes shortened

wings bent

maxillary palp elongated

larva lethality

cuticle remnants without visible segmentation, differentiation or axis

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

larval musculature irregular

larva number hatched none (< 10 %)


larva lethality

abdomen circumference decreased

larval musculature mostly not present

It seems that only one specific muscle in the head is still present.

larva number hatched none (< 10 %)

adult lethality


larva lethality


adult lethality

adult lethality

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

elytra not closed

larva lethality

adult lethality


adult lethality

ovary without detectable defects

egg production: number no/almost no eggs

larva lethality

egg production: number <50% of expected

ovary without detectable defects

mature egg deposition 5 >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


larva lethality late (L7)

pupal molt delayed (still prepupa)

larva lethality

pupa eclosion not fulfilled (died as pupa)


larva lethality

adult lethality


ovary without detectable defects

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality

larva lethality

abdomen everted (inside-out)

thorax segment posterior everted (inside-out)

thorax & abdomen mostly everted (inside-out)

larval musculature irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

egg shell surface irregular

adult lethality


adult lethality

adult lethality


adult lethality

larva lethality


larva lethality

abdomen dorsal bent

abdomen mostly everted (inside-out)

legs slightly shape irregular

head capsule slightly size decreased

adult lethality


adult lethality

adult lethality

larva lethality

adult lethality


larva lethality

abdomen segment number decreased

thorax partially not present

thorax & abdomen partially not present

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number few (< 50 %) hatched


eclosion delayed (still pupa)

adult lethality

larva lethality

labium partially not present

abdomen segment number # of remaining segments  decreased

legs sometimes shape irregular

head capsule mostly not present

larva number few (< 50 %) hatched


adult lethality

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

wings sometimes blistered

pupal molt delayed (still prepupa)

larva lethality

vertex triplet vertex triplet posterior not present

gena triplet seta number increased

head capsule sometimes size decreased

abdomen shape irregular

head appendages sometimes shape irregular

larva appendages partially shortened

larval musculature pattern irregular

larva number hatched none (< 10 %)

eclosion not fulfilled (with pupal and adult features)


larva lethality

larva number hatched many (ca. 70-100 %)


eclosion not fulfilled (died as pupa)

oblique dorsal thoracic muscles shortened

wings shortened

pupal molt not fulfilled (died as prepupa)

larva lethality

prothorax mostly not present

labium partially not present

head & thorax partially not present

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

larva number hatched none (< 10 %)

adult lethality

adult lethality


fertility after larval injection decreased (no living offspring)

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

club cuticularisation decreased

adult lethality


larva lethality

egg production: number no/almost no eggs

adult nutritional state bad (cachectic)


pupal molt not fulfilled (died as L7 or prepupa)

abdomen posterior remaining

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


larva lethality


adult lethality

ovary without detectable defects

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality

elytra not closed

larva lethality

larva number hatched none (< 10 %)


abdominal odoriferous glands darker

prothoracic odoriferous glands darker

adult lethality

ovary without detectable defects

egg production: number <50% of expected

larva number hatched none (< 10 %)

egg production: number <50% of expected


larva lethality

antenna unilateral not present

procephalic head partially not present

head capsule size decreased


adult lethality

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality

labium mostly not present

head capsule size decreased


adult lethality

adult lethality

labrum size decreased

mandible potentially transformed

mandible not present

maxilla number increased

affected eggs/embryos/larvae

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality


pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

larva lethality

larva constricted

abdomen anterior not present

thorax partially posterior not present

head partially not present

larva cuticle partially not present

abdomen partially remaining

cuticle remnants cuticle remnants with unidentifyable segments

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larval musculature irregular

ovary without detectable defects

mature egg deposition 4 >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


larva lethality

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


larva lethality

adult lethality


pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality


eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality

pupa eclosion not fulfilled (died as pupa)


pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

larva lethality


larva lethality

head & thorax partially not present

head & thorax partially constricted

larva appendages shape irregular

head & thorax & abdomen mostly not present

head appendages position irregular

cuticle remnants without visible segmentation, differentiation or axis

larval musculature irregular

larva number few (< 50 %) hatched


adult lethality

larva lethality

tropharium slightly shape irregular

egg chamber mostly not present

tropharium shortened

vitellarium shortened


ovary without detectable defects

egg production: number <50% of expected

larva lethality

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality

larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

adult nutritional state bad (cachectic)

adult lethality

elytra distal bent

legs darker

elytra darker

funicle darker

wings distal bent

larva lethality

larval musculature fluorescence decreased

ovary without detectable defects

egg production: number <50% of expected

egg production: number <50% of expected


adult lethality

adult lethality

larva lethality

head mostly remaining

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larval musculature pattern irregular

embryo/egg not developed/not fertilized


larva lethality

head & thorax & abdomen partially not present

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

adult lethality


pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality

adult lethality


pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt not fulfilled (died with pupal and larval features)

pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still larva)

larva lethality

mature egg deposition 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number no/almost no eggs


eclosion delayed (still pupa)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality

eclosion not fulfilled (died as pupa)

adult lethality


fertility after larval injection decreased (no living offspring)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

larva lethality

thorax & abdomen bristle pattern irregular

thorax & abdomen seta pattern irregular

affected eggs/embryos/larvae

adult lethality

adult lethality

larva number few (< 50 %) hatched


eclosion not fulfilled (died as pupa)

larva lethality

adult lethality

adult lethality


ovary without detectable defects

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

pupal molt delayed (still larva)

larva lethality

adult lethality


larva lethality


larva lethality late (L7)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

larva lethality

labium distal surface irregular

stripes are missing

maxillary palp surface irregular

stripes are missing

labium swollen

antenna sometimes shape irregular

legs shortened

legs tibiotarsus potentially fused

legs tibiotarsus size decreased

leg musculature pattern irregular

abdomen tracheal opening not present

legs trochanter size decreased

labrum not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva lethality late (L7)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult nutritional state bad (cachectic)


adult lethality

larva lethality

abdomen segment number decreased

head & thorax partially not present

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


larva lethality

adult lethality

adult lethality

adult lethality


eclosion not fulfilled (died as pupa)

wings shape irregular

abdomen circumference decreased

pronotum elongated

pupal molt delayed (still larva)

larva lethality

legs shape irregular

head bristle pattern irregular

ventral vt to close to dorsal gt

head appendages partially orientation irregular

thorax segment number decreased

head appendages partially not present

head & thorax segment partially not present

affected eggs/embryos/larvae

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality

larva number hatched many (ca. 70-100 %)


eclosion not fulfilled (died as pupa)

adult lethality


larva lethality

affected eggs/embryos/larvae


eclosion not fulfilled (died as pupa)

larva lethality

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

eclosion not fulfilled (died as pupa)


ovary without detectable defects

egg production: number <50% of expected

larva lethality

egg production: number >150% of expected

eclosion not fulfilled (died as pupa)


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality


adult lethality

larva lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality


adult lethality

adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

adult lethality


adult lethality

larva lethality

affected eggs/embryos/larvae

adult lethality


ovary without detectable defects

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (died as pupa)

eclosion not fulfilled (not completely eclosed)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

egg production: number <50% of expected

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected


larva lethality

adult lethality

eclosion not fulfilled (died as pupa)


ovary without detectable defects

egg production: number <50% of expected

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

adult lethality

legs trochanter potentially fused

legs femur potentially fused

legs femur shape irregular

legs tibiotarsus shape irregular

legs shape irregular

head appendages partially shape irregular

larva appendages partially shape irregular

affected eggs/embryos/larvae

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

adult lethality

adult lethality


larva lethality

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

adult lethality

adult lethality

adult lethality


eclosion not fulfilled (died as pupa)

abdomen gin-traps slightly shortened

elytra orientation irregular

antenna orientation irregular

larva lethality

adult lethality

affected eggs/embryos/larvae

larval musculature fluorescence decreased

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


larva lethality

adult lethality

adult lethality


larva lethality

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (died as pupa)


ovary without detectable defects

mature egg deposition 2 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

adult nutritional state potentially bad (cachectic)

egg production: number no/almost no eggs


larva lethality

larva segment sometimes fused

larva segment random number decreased

affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


larva lethality

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

antenna distal broader

maxillary palp broader

labium distal broader

affected eggs/embryos/larvae

adult lethality

leg musculature partially not present

thorax musculature potentially rounded

head musculature potentially rounded

abdomen musculature degenerated

thorax musculature partially degenerated

head musculature degenerated

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

larva number hatched none (< 10 %)


adult nutritional state

eclosion delayed (still pupa)

pupal molt not fulfilled (died as prepupa)

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

larva lethality

adult lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


adult lethality

tropharium shortened

mature egg not present

egg chamber number decreased

adult nutritional state bad (cachectic)

eclosion delayed (still pupa)

larva lethality

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

egg chamber mostly not present

vitellarium mostly decreased

larva number hatched none (< 10 %)


larva lethality

larva number hatched none (< 10 %)

egg shell structure irregular

potentially dried out due to defects

embryo/egg not developed/not fertilized

egg size decreased

affected eggs/embryos/larvae

adult lethality

eclosion not fulfilled (died as pupa)


larva lethality

eclosion not fulfilled (died as pupa)


adult nutritional state

eclosion delayed (still pupa)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult nutritional state bad (cachectic)


larva lethality

adult lethality

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected


eclosion delayed (still pupa)

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

thorax & abdomen mostly not present

mesothorax not present

abdomen complete not present

abdomen segment mostly not present

metathorax not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

eclosion delayed (still pupa)

abdominal odoriferous glands content fragmented

abdominal odoriferous glands darker

prothoracic odoriferous glands darker

adult lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected


adult lethality

larva lethality

abdomen segment number decreased

cuticle remnants strongly fragmented (cuticle crumbs)

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

eclosion not fulfilled (with pupal and adult features)

eclosion not fulfilled (died as pupa)


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality

adult lethality


larva lethality

larva everted (inside-out)

prothorax not present

head complete not present

abdomen partially everted (inside-out)

thorax segment partially not present

head partially random not present

larva segment anterior decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


larva lethality

adult lethality

adult lethality

adult nutritional state bad (cachectic)

adult lethality

adult lethality


adult lethality

larva lethality

larva number hatched many (ca. 70-100 %)


adult lethality

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult nutritional state bad (cachectic)


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality

adult lethality

adult nutritional state bad (cachectic)


larva lethality


pupal molt not fulfilled (died with pupal and larval features)

larva lethality

larva lethality

prothoracic odoriferous glands darker

egg chamber ap-orientation irregular

egg chambers growing potentially in the tropharium

egg chamber potentially number decreased

tropharium shortened

vitellarium shortened

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


larva lethality

adult lethality


eclosion not fulfilled (died as pupa)

abdomen gin-traps not present

female genital lobes shortened

elytra size shortened

larva lethality

larval musculature partially not present

larval musculature sometimes orientation irregular

larval musculature partially thinner

affected eggs/embryos/larvae

egg production: number <50% of expected

ovary without detectable defects

larva number hatched none (< 10 %)


adult lethality

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

larva lethality

ovary without detectable defects

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


larva lethality

legs sometimes shortened

legs sometimes shape irregular

head appendages sometimes shape irregular

larva appendages irregular

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (died as pupa)


ovary without detectable defects

egg production: number <50% of expected

adult lethality

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number no/almost no eggs

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

adult lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

adult lethality


larva lethality

adult lethality

adult lethality


adult lethality

larva lethality

adult lethality


ovary without detectable defects

egg production: number <50% of expected


larva lethality late (L7)

pupal molt delayed (still larva)

adult lethality

adult lethality

adult nutritional state bad (cachectic)

adult lethality


larva lethality

larva bent

most of the time still in egg

affected eggs/embryos/larvae

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


larva lethality

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched


eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality


larva lethality

legs shortened

larva segment dorsoventral-axis thinner

legs shape irregular

larva everted (inside-out)

affected eggs/embryos/larvae

larval musculature mostly not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

legs shape irregular

thorax & abdomen segment circumference thinner

abdomen segment number decreased

legs size decreased

Femur and Tibiotarsus potentially fused.

thorax & abdomen circumference irregular

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


eclosion not fulfilled (died as pupa)

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality

adult lethality


elytra shortened

elytra shortened

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


larva lethality

adult lethality


pupal molt delayed (still larva)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality

adult lethality

adult lethality

adult lethality


adult nutritional state

larva lethality

abdomen segment number decreased

thorax segment posterior fused

affected eggs/embryos/larvae

larval musculature dorsal partially orientation irregular

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched


larva lethality

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

egg production: number <50% of expected

adult lethality

ovary without detectable defects

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality

larva number hatched many (ca. 70-100 %)

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (died as pupa)


ovary without detectable defects

scutellum potentially broader

elytra surface blistered

head appendages partially random not present

affected eggs/embryos/larvae


larva lethality

larva segment number decreased

cuticle remnants strongly fragmented (cuticle crumbs)

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number >150% of expected


adult lethality

elytra not closed

larva lethality

legs tibiotarsus potentially fused

legs femur potentially fused

legs tibiotarsus size decreased

legs femur size decreased

legs trochanter size decreased

legs coxa size decreased

labium shortened

legs shortened

maxillary palp shortened

larva appendages partially shortened

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

ovary without detectable defects

mature egg deposition 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number few (< 50 %) hatched

egg production: number <50% of expected


larva lethality

mandible not present

head capsule not present

labrum not present

antenna not present

head appendages partially not present

affected eggs/embryos/larvae

adult lethality


larva lethality

abdomen segment number decreased

sometimes fragments left

legs partially shape irregular

antenna flagellum not present

affected eggs/embryos/larvae

larval musculature partially random not present

larval musculature pattern irregular

affected eggs/embryos/larvae

egg production: number <50% of expected

larva number hatched none (< 10 %)

adult lethality

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult nutritional state

larva lethality


egg chamber number decreased

adult nutritional state bad (cachectic)

larva lethality

affected eggs/embryos/larvae

ovary without detectable defects

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected


adult lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

wings not present

eclosion delayed (still pupa)

prosternal process broader

elytra not closed

labrum shortened

pupal molt delayed (still larva)

larva lethality

ovary without detectable defects

adult lethality

adult nutritional state potentially bad (cachectic)

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality


adult lethality

larva lethality


adult lethality

adult lethality

adult lethality


adult lethality


fertility after larval injection decreased (no living offspring)

adult lethality

affected eggs/embryos/larvae

mature egg not present

egg production: number <50% of expected

ovary without detectable defects

larva number hatched none (< 10 %)


pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

larva lethality

adult lethality

mature egg not present

adult lethality


adult lethality

tropharium size decreased

tropharium shape irregular

larva lethality

adult lethality

adult lethality


larva lethality

adult lethality


larva lethality

adult lethality

larva number few (< 50 %) hatched

adult lethality


elytra surface irregular

larva lethality

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality


adult lethality

adult lethality

larva lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

egg production: number <50% of expected

ovary without detectable defects

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected


adult lethality

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

elytra shortened

wings shortened

pupal molt delayed (still prepupa)

larva lethality

adult lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


adult lethality

ovary without detectable defects

hindwing folding not present

adults open and close elytra the hole time (trying to fold hindwings proper)

adult lethality

affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

larva lethality

abdomen segment number decreased

abdomen constricted

abdomen everted (inside-out)

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

eclosion not fulfilled (with pupal and adult features)


adult lethality

adult lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

larva number hatched many (ca. 70-100 %)


ovary without detectable defects

egg production: number <50% of expected

larva lethality

larva segment central not present

affected eggs/embryos/larvae

egg chamber mostly not present

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

cuticle remnants with some identifiable segments

head & thorax & abdomen segment random not present

cuticle remnants strongly fragmented (cuticle crumbs)

affected eggs/embryos/larvae

larval musculature random not present

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

wings distal bent

wings shortened

antenna shortened

pupal molt delayed (still larva)

larva lethality

adult lethality

adult nutritional state bad (cachectic)

adult lethality

adult lethality


larva lethality

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched


adult lethality

motility decreased

moving their legs very slow and aren't crawling around


adult lethality


larva lethality


adult lethality

adult lethality

adult lethality

larva segment posterior random not present

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

larva lethality

adult lethality

adult lethality


larva lethality

adult lethality


ovary without detectable defects

egg production: number <50% of expected

larva lethality

head & thorax & abdomen segment sometimes not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 4 >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected


larva lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

adult lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality

eclosion not fulfilled (died as pupa)

eclosion not fulfilled (died as pupa)

wings blistered

antenna orientation irregular

larva lethality

adult lethality

head segment random not present

affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

ovary without detectable defects

mature egg number increased

lateral oviduct always empty

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


adult lethality

larva lethality


larva lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality

adult lethality


larva lethality

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality

larva everted (inside-out)

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

eclosion not fulfilled (died as pupa)

adult lethality


ovary without detectable defects

egg production: number <50% of expected

adult lethality

larva lethality

head capsule not present

head transformed

thorax partially duplicated

abdomen partially duplicated

head appendages mostly not present

legs number increased

legs sometimes fused

antenna not present

labrum not present

abdomen segment number increased

thorax segment number increased

head size decreased

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality


adult lethality

larva lethality

abdomen partially everted (inside-out)

abdomen posterior thinner

abdomen segment number decreased

legs shape irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva lethality

adult lethality

adult lethality

adult lethality


larva lethality

adult lethality

adult lethality


adult lethality

adult lethality

adult lethality

elytra shape irregular

elytra not closed

larva lethality

elytra sometimes blistered

elytra shortened

adult lethality

adult lethality


adult lethality


adult lethality

ovary without detectable defects

egg production: number <50% of expected

adult nutritional state bad (cachectic)

elytra surface irregular

elytra size decreased

wings folding not present

elytra not closed

prothorax bristle number decreased

abdomen gin-trap setae number decreased

wings not closed

larva lethality


mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number no/almost no eggs

legs pretarsus cuticularisation decreased

legs tarsus cuticularisation decreased

legs tibia cuticularisation decreased

larva lethality

larva number few (< 50 %) hatched

adult lethality


larva lethality


adult lethality

prothorax bristle number decreased

larval musculature fluorescence decreased

affected eggs/embryos/larvae


fertility after larval injection decreased (no living offspring)

adult lethality

ovary without detectable defects

egg production: number <50% of expected

adult lethality

adult nutritional state bad (cachectic)

elytra surface irregular

elytra blistered

adult lethality

larva everted (inside-out)

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched

adult lethality

adult lethality immediately after eclosion


adult lethality

adult lethality

larva lethality

adult lethality


larva lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

adult lethality

eclosion not fulfilled (died as pupa)

club distal elongated

last club segment elongated

female genital lobes rounded

adult lethality

larva everted (inside-out)

affected eggs/embryos/larvae

adult lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

adult lethality

adult lethality


larva lethality

larva segment random not present

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality


pupal molt not fulfilled (died as L7 or prepupa)

pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

larva lethality

adult lethality

adult lethality

adult lethality

adult lethality


larva lethality

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

egg production: number <50% of expected


adult lethality

larva lethality

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva lethality

larva number hatched many (ca. 70-100 %)

eclosion not fulfilled (with pupal and adult features)


larva lethality

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


pupal molt delayed (still larva)

every thoratic segment was separatly blistered on the outer side. 2 blisters per segment in all dead prepupa

pupal molt delayed (still prepupa)

thorax partially blistered

pupal molt delayed (still larva)

larva lethality

adult lethality

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult nutritional state potentially bad (cachectic)


adult lethality

adult lethality

eclosion not fulfilled (died as pupa)


adult lethality

larva lethality


adult lethality

larva lethality

adult lethality

adult lethality

adult lethality


adult lethality

larva lethality

adult lethality

adult lethality

head & thorax & abdomen appendages random not present

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

larva lethality

adult lethality

adult lethality


adult lethality

larva lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

pupa eclosion not fulfilled (died as pupa)


larva lethality

adult lethality

larva lethality

adult lethality

vitellarium mostly not present

adult lethality

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

egg production: number <50% of expected


larva lethality

affected eggs/embryos/larvae

adult lethality


larva lethality

adult lethality


larva lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality

adult lethality

larva lethality late (L7)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

larval musculature pattern irregular

15 of 29 (51,72%)

affected eggs/embryos/larvae

egg production: number <50% of expected


larva lethality


ovary without detectable defects

egg production: number <50% of expected

larva lethality

larva number hatched none (< 10 %)


larva lethality late (L7)

pupal molt delayed (still larva)

adult lethality

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality


larva lethality

head appendages rounded

legs podomer slightly shortened

head appendages shortened

larva appendages shortened

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

adult lethality


larva lethality

adult lethality

adult lethality


eclosion delayed (still pupa)

eclosion delayed (still pupa)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality

adult lethality

adult lethality


adult lethality

adult nutritional state bad (cachectic)

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

adult lethality


pupal molt delayed (still prepupa)

larva lethality

affected eggs/embryos/larvae

larval musculature irregular

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality

adult lethality


adult lethality

larva lethality

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

legs pretarsus distal rounded

larva seta not present

legs pretarsus shortened

head potentially bent

larva bristle not present

adult lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

adult lethality


larva lethality

larva number hatched none (< 10 %)


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

adult lethality

adult lethality

adult nutritional state bad (cachectic)

adult lethality

egg production: number <50% of expected


larva lethality

head appendages partially not present

maxilla and labium seem to be less affected than the other head appendages.

head appendages size decreased

mandible not present

prothorax partially not present

head capsule not present

affected eggs/embryos/larvae

larval musculature irregular

larva number hatched none (< 10 %)


adult lethality

elytra colour irregular

elytra surface irregular

elytra not closed

larva lethality

thorax musculature irregular

because nearly all embryos are lying lateral, it is not possible to say what exactly is wrong with the thoracic muscles, only that there is soething wrong.leg muscles seem to be not affected (at least they are not rounded or missing)

abdomen musculature partially rounded

abdomen musculature partially random not present

larva number hatched none (< 10 %)

adult lethality


elytra not closed

adult lethality

larva lethality

legs podomer potentially fused

legs podomer shortened

head & thorax & abdomen partially not present

abdomen segment number decreased

thorax partially not present

head appendages random not present

larva everted (inside-out)

upended larvae show also missing abdominal and thoracic segments.

appendages partially remaining

embryo/egg without visible larval cuticle in cuticle preparation

larval musculature irregular

larva number few (< 50 %) hatched


adult lethality

legs tibia shortened

legs shape irregular

Femur and tibia of the thoracic legs thicker.

elytra dorsal not closed

legs sometimes swollen

legs tarsus shape irregular

antenna shape irregular

legs sometimes size shortened

elytra partially necrotic

legs shape irregular

elytra ventral not closed

female genital lobes rounded

elytra shortened

antenna shape irregular

Antennal club thickened.

larva lethality

tropharium shape irregular

egg chamber mostly not present

adult lethality

adult lethality


larva lethality

adult lethality


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

oblique dorsal thoracic muscles slightly median shortened

elytra not closed

larva lethality

ovary without detectable defects

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

adult lethality


larva lethality

larva lethality

adult lethality

head capsule mostly not present

procephalic head not present

head split

head not present

affected eggs/embryos/larvae

larval musculature irregular


adult lethality

eclosion delayed (still pupa)

adult lethality

head appendages orientation irregular

larva cuticle dorsal not closed

abdomen segment number decreased

thorax partially not present

head capsule size decreased

affected eggs/embryos/larvae

larval musculature irregular

larva number few (< 50 %) hatched

adult lethality immediately after eclosion


adult lethality

adult lethality

legs podomer shortened

abdomen bristle pattern lighter

thorax partially not present

head capsule mostly not present

head & thorax & abdomen irregular

head appendages orientation irregular

larval musculature irregular

larva number few (< 50 %) hatched


adult lethality

larva lethality


larva lethality

legs podomer shortened

gnathal appendages random partially not present

procephalic appendages not present

abdomen segment number decreased

thorax partially not present

head capsule not present

larval musculature irregular

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

larva lethality

larva number hatched many (ca. 70-100 %)

eclosion not fulfilled (with pupal and adult features)


adult lethality

larva lethality

adult lethality


eclosion not fulfilled (died as pupa)

eyes shape irregular

elytra not closed

legs potentially partially necrotic

elytra shortened

elytra partially necrotic

hindwing partially sometimes necrotic

urogomphi shape irregular

antenna orientation irregular

larva lethality

ovary without detectable defects

larva number hatched none (< 10 %)


ovary without detectable defects

egg production: number <50% of expected

adult lethality

larva number few (< 50 %) hatched

adult lethality

adult lethality


ovary without detectable defects

hindwing folding irregular

elytra size increased


adult lethality

larva lethality

affected eggs/embryos/larvae

vitellarium shortened

previtellogenic egg chamber mostly not present

egg chamber number decreased

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected


larva lethality

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality

larva everted (inside-out)

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


elytra surface irregular

elytra not closed

larva lethality

cuticle everted (inside-out)

cuticle remnants without visible segmentation, differentiation or axis

larval musculature irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

larva number hatched none (< 10 %)

adult lethality

adult lethality


adult lethality

eclosion delayed (still pupa)

eclosion not fulfilled (died as pupa)

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

adult nutritional state bad (cachectic)

egg production: number <50% of expected


larva lethality

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

affected eggs/embryos/larvae

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

abdomen partially everted (inside-out)

larva bent

affected eggs/embryos/larvae

larval musculature irregular

previtellogenic egg chamber potentially fused

egg chamber mostly not present

mature egg not present

egg production: number no/almost no eggs

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

abdomen partially everted (inside-out)

larva bent

affected eggs/embryos/larvae

larval musculature irregular

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

abdomen partially everted (inside-out)

larva bent

affected eggs/embryos/larvae

larval musculature irregular


adult lethality

larva lethality

larval musculature irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number hatched none (< 10 %)

adult lethality


eclosion delayed (still pupa)

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

larva number hatched none (< 10 %)

adult lethality


larva lethality

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)


elytra not closed

adult lethality

urogomphi orientation irregular

urogomphi size decreased

affected eggs/embryos/larvae

some larva developed to L2 larva and than died. looked normal and couldn't go through sieve (maybe CNS defect)

larva number hatched none (< 10 %)


adult lethality

legs femur shortened

elytra surface irregular

elytra not closed

female genital lobes shortened

legs shortened

antenna orientation irregular

abdomen gin-traps orientation irregular

abdomen gin-traps slightly size decreased

Gin traps pointing to dorsal instead of lateral. Whole gin trap closely attached to the trunk.

elytra shortened

larva lethality

adult lethality


adult lethality

eclosion delayed (still pupa)

head split

head capsule not present

head appendages shape irregular

posterior terminus partially remaining

prothorax not present

procephalic head not present

head & thorax & abdomen partially not present

gut remaining

cuticle remnants with some identifiable segments

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature irregular

larva number few (< 50 %) hatched


antenna orientation irregular

larva lethality

gut mostly remaining

appendages random partially remaining

affected eggs/embryos/larvae

larval musculature irregular

adult lethality

adult lethality


larva lethality


larva lethality

pygopodium not present

legs pretarsus not present

legs podomer rounded

head appendages shortened

larva appendages shape irregular

urogomphi size decreased

legs podomer shortened

head appendages shape rounded

larva appendages shortened


adult lethality

mature egg deposition 4 5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

ovary without detectable defects

larva number hatched none (< 10 %)


elytra surface irregular

adult lethality immediately after eclosion

larva lethality

larva number hatched many (ca. 70-100 %)

eclosion not fulfilled (with pupal and adult features)


adult lethality

larva lethality

larva appendages rounded

legs pretarsus shortened

head capsule mostly not present

larva appendages shortened

affected eggs/embryos/larvae

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

procephalic appendages sometimes not present

gnathal appendages mostly not present

larva appendages partially not present

abdomen segment number decreased

thorax partially not present

head capsule size decreased

head & thorax partially not present

larval musculature irregular

larva number few (< 50 %) hatched


eclosion delayed (still pupa)

larva lethality

ovary without detectable defects

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality

larva lethality

larva number few (< 50 %) hatched

adult lethality


adult lethality

elytra not closed

larva lethality

legs partially remaining

abdomen partially remaining

posterior terminus mostly remaining

gut remaining

labrum mostly remaining

antenna mostly remaining

affected eggs/embryos/larvae

larval musculature irregular

larva number hatched none (< 10 %)


adult lethality

eclosion delayed (still pupa)

larva number few (< 50 %) hatched

adult lethality


adult lethality

larva lethality

larva number few (< 50 %) hatched


eclosion delayed (still pupa)

larva lethality late (L7)

pupal molt delayed (still larva)

larva number few (< 50 %) hatched

adult lethality


ovary without detectable defects

larva cuticle dorsal not closed

larva dorsal bent

abdomen partially everted (inside-out)

larva complete everted (inside-out)

affected eggs/embryos/larvae

larval musculature irregular

larva number hatched none (< 10 %)


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


adult lethality

larva lethality

abdomen partially everted (inside-out)

head appendages random not present

cuticle partially everted (inside-out)

abdomen segment number decreased

thorax partially not present

head capsule mostly not present

head & thorax & abdomen irregular

appendages random partially remaining

larval musculature irregular

larva number few (< 50 %) hatched


adult lethality

elytra not closed

elytra surface irregular

elytra colour irregular

legs podomer shortened

head bristle pattern irregular

antenna antennal flagellum not present

head appendages random not present

head appendages orientation irregular

head appendages shape irregular

abdomen segment number decreased

thorax partially not present

head capsule size decreased

head & thorax partially not present


pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality

larva lethality


larva lethality


adult lethality

larva lethality

thorax complete not present

head capsule size decreased

abdomen segment number decreased

thorax partially not present

head appendages sometimes random not present

head & thorax partially not present

larval musculature irregular

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


elytra sometimes not closed

elytra surface irregular

adult lethality

appendages partially random remaining

cuticle remnants strongly fragmented (cuticle crumbs)

penetrance about 50 %

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

ovary without detectable defects

larva number few (< 50 %) hatched

egg production: number <50% of expected


larva lethality

larva number few (< 50 %) hatched

adult lethality


eclosion delayed (still pupa)

larva lethality


adult lethality

elytra surface irregular

larva lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

legs pretarsus shape irregular

maxilla shape irregular

legs tibiotarsus not present

antenna distal not present

larva appendages irregular

affected eggs/embryos/larvae


wings size decreased

wings shape irregular

legs size decreased

legs shape irregular

eclosion not fulfilled (died as pupa)

pronotum not present

antenna size shortened

larva lethality

thorax potentially blistered

pupal molt not fulfilled (died with pupal and larval features)

thorax potentially irregular

larva lethality

All 10 larvae show necrotic blister near the thoracic region. Pupal features are not clearly recognizable and the annotations are not certain because of this.

gena triplet complete irregular

antenna antennal flagellum not present

maxilla distal size increased

pygopodium segmentation/division irregular

the proximal part of the pygopodia, the base, with the bristles is mostly absent, and the distance between the two pygopodia is increased

urogomphi shape irregular

thorax podomer broader

antenna segment distal broader

also the sensory organ on the middle antennal segment is dublicated most of the time

larva appendages shape irregular

antenna antennal flagellum not present

antenna pedicellus shape irregular

antenna distal shape irregular

gena triplet bristle number increased

head bristle pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

eclosion not fulfilled (not completely eclosed)

eclosion not fulfilled (died as pupa)

ovipositor elongated

elytra not closed

oblique dorsal thoracic muscles median partially fragmented

oblique dorsal thoracic muscles median partially not present

abdomen circumference increased

longitudinal dorsal thoracic muscles fluorescence decreased

larva lethality

cuticle remnants without visible segmentation, differentiation or axis

embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larva number hatched none (< 10 %)

adult lethality


fertility after larval injection decreased (no living offspring)

larva lethality

larva number hatched none (< 10 %)

adult lethality

egg production: number <50% of expected


eclosion not fulfilled (died as pupa)

larva number hatched many (ca. 70-100 %)


larva lethality

adult lethality

adult lethality

adult lethality

larva lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

larva lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality

abdomen segment partially fused

head & thorax & abdomen partially not present

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched


pupal molt not completely molted

larva lethality

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


larva lethality

adult lethality


adult lethality immediately after eclosion

larva lethality

adult lethality


adult lethality

larva lethality


larva lethality

adult lethality

larval musculature fluorescence decreased

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


adult lethality

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number hatched none (< 10 %)

adult lethality


ovary without detectable defects

egg production: number <50% of expected

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

abdomen partially everted (inside-out)

cuticle remnants with some identifiable segments

sometimes insideout

abdomen partially not present

larva everted (inside-out)

head partially remaining

cuticle remnants strongly fragmented (cuticle crumbs)


larva lethality

cuticle remnants with unidentifyable segments

adult lethality

eclosion not fulfilled (died as pupa)


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

egg production: number no/almost no eggs

adult lethality

adult lethality


pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality

adult lethality

adult lethality


ovary without detectable defects

egg production: number <50% of expected

pupal molt delayed (still larva)

larva lethality

adult lethality


adult lethality

larva lethality

adult lethality


larva lethality late (L7)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

larva number hatched none (< 10 %)

embryo/egg not developed/not fertilized


pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still prepupa)

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


tropharium potentially not present

vitellarium not present

egg chamber not present

tropharium size decreased

larva lethality


eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

adult lethality


adult lethality

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larval musculature irregular


ovary without detectable defects

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

larva lethality

larva anterior not present

head mostly not present

larva everted (inside-out)

partially or complete insideout

embryo/egg without visible larval cuticle in cuticle preparation

larval musculature partially not present

larval musculature partially orientation irregular

larval musculature thinner

larval musculature irregular

larva number few (< 50 %) hatched

adult lethality

labium sometimes not present

labrum size decreased

head appendages orientation irregular

head capsule shape irregular

head capsule size decreased

head capsule not present

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

egg colour darker

larva number few (< 50 %) hatched

adult lethality


ovary without detectable defects

egg production: number <50% of expected

larva lethality

larva anterior not present

head mostly not present

larva everted (inside-out)

partially or complete insideout

larval musculature thinner

larval musculature irregular

larva number few (< 50 %) hatched

adult lethality

labium sometimes not present

labrum size decreased

head appendages orientation irregular

head capsule shape irregular

head capsule size decreased

head capsule not present

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

egg colour darker

larva number few (< 50 %) hatched

adult lethality


adult lethality

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

abdomen mostly not present

abdominal segment 1 present

legs shortened

larva number hatched many (ca. 70-100 %)


fertility after larval injection decreased (no living offspring)

adult lethality

larva lethality late (L7)

adult lethality

pupal molt delayed (still larva)

larva lethality

egg production: number <50% of expected

eclosion not fulfilled (with pupal and adult features)

eclosion not fulfilled (died as pupa)


larva lethality

adult lethality

adult lethality


larva lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality


larva lethality late (L7)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality


adult lethality

adult lethality

larva lethality

adult lethality


larva lethality

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

larva lethality early (L5/6)

adult lethality

adult lethality

pupa eclosion not fulfilled (died as pupa)


eclosion not fulfilled (died as pupa)

larva lethality

larva lethality early (L5/6)

adult lethality

adult lethality

adult lethality

adult nutritional state potentially bad (cachectic)

adult nutritional state bad (cachectic)

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

larva lethality

larva lethality early (L5/6)

adult lethality

adult lethality

adult lethality

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

larva lethality early (L5/6)

adult lethality

adult lethality


pupal molt delayed (still prepupa)

larva lethality


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

head & thorax & abdomen partially not present

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larval musculature irregular

larva number few (< 50 %) hatched


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

vitellarium shape irregular

mature egg not present

previtellogenic egg chamber stage sequence irregular

vitellogenic egg chamber mostly not present

egg production: number no/almost no eggs

adult lethality


adult lethality

eclosion delayed (still pupa)

head & thorax complete not present

head & thorax partially not present

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

larval musculature fluorescence decreased

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

larva lethality


larva lethality late (L7)

pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

larva lethality

embryo/egg not developed/not fertilized

egg chamber not present

larva number hatched none (< 10 %)

adult lethality

adult lethality


ovary without detectable defects

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

larva lethality

adult lethality


pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality

adult lethality


adult lethality

ovary without detectable defects

motility decreased

larva lethality


larva lethality

adult lethality

head & thorax & abdomen segment random not present

head & thorax & abdomen segment shape irregular

Few extra potentially head appendages are present in abdomen segment.

head appendages random not present

thorax & abdomen segment constricted

Constriction occurs between thorax and abdomen segments.

abdomen segment posterior dorsal bent

head capsule not present

thorax & abdomen segment fragmented

Fragmentation separates thorax and abdomen parts.

cuticle remnants with unidentifyable segments

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


larva lethality

larva number hatched many (ca. 70-100 %)


ovary without detectable defects

adult nutritional state bad (cachectic)

eclosion not fulfilled (died as pupa)

adult lethality

eclosion not fulfilled (not completely eclosed)

eclosion not fulfilled (with pupal and adult features)

eclosion delayed (still pupa)

eclosion not fulfilled (died as pupa)

larva lethality

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


larva lethality

head & thorax & abdomen partially not present

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg with embryonic tissue, no cuticle, no fluorescence

embryo/egg not developed/not fertilized

larval musculature irregular

larva number few (< 50 %) hatched


larva lethality

adult lethality

adult lethality


pupal molt delayed (still prepupa)

larva lethality

adult lethality

adult nutritional state bad (cachectic)

egg production: number no/almost no eggs

adult lethality


adult lethality

ovary without detectable defects

pupal molt delayed (still prepupa)

larva lethality

embryo/egg not developed/not fertilized

eclosion not fulfilled (died as pupa)


larva lethality

legs coxa potentially fused

legs trochanter potentially fused

legs segmentation/division irregular

legs shortened

abdomen musculature partially not present

larva number few (< 50 %) hatched

adult lethality


larva lethality

labium partially not present

abdomen segment number decreased

abdomen posterior not present

gnathal appendages sometimes shape irregular

thorax & abdomen partially not present

cuticle remnants with some identifiable segments


larva lethality


fertility after larval injection decreased (no living offspring)

adult lethality

larva lethality

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


ovary without detectable defects

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

adult lethality

adult lethality immediately after eclosion

abdominal segment 8  ventral median spotted

larva lethality


larva lethality

sometimes inside out

cuticle remnants strongly fragmented (cuticle crumbs)

head partially not present

larva anterior mostly not present

embryo/egg without visible larval cuticle in cuticle preparation

larval musculature irregular

adult lethality

head appendages sometimes random not present

affected eggs/embryos/larvae

larva number hatched many (ca. 70-100 %)


larva lethality

sometimes inside out

head partially not present

larva anterior mostly not present

larval musculature irregular

adult lethality

larva number hatched none (< 10 %)


larva lethality

larva anterior mostly not present

abdomen dorsal not closed

thorax partially not present

larval musculature irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larva number few (< 50 %) hatched

adult lethality

eclosion not fulfilled (with pupal and adult features)


eclosion not fulfilled (died as pupa)

pupal molt not completely molted

larva lethality


adult lethality

adult nutritional state bad (cachectic)

adult lethality

pronotum surface irregular

elytra surface irregular

hindwing folding irregular

elytra not closed

larva lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


previtellogenic egg chamber sometimes deposition present

mature egg not present

previtellogenic egg chamber segmentation/division irregular

tropharium size decreased

tropharium shape irregular

egg production: number <50% of expected

larva lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number no/almost no eggs


adult lethality

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

pupal molt delayed (still prepupa)

larva number hatched none (< 10 %)

larval musculature irregular

embryo/egg not developed/not fertilized

adult lethality


larva lethality

adult lethality


adult lethality

eclosion delayed (still pupa)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality

abdomen segment number decreased

affected eggs/embryos/larvae

larval musculature fluorescence decreased

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


eclosion delayed (still pupa)

larva lethality


larva lethality

ovary without detectable defects

mature egg deposition 3 >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

embryo/egg not developed/not fertilized

egg production: number <50% of expected


ovary without detectable defects

egg production: number <50% of expected

larva lethality


adult lethality

adult lethality

eclosion not fulfilled (not completely eclosed)

larva lethality

abdomen posterior partially remaining

some head appendages are mostly attached to it

cuticle remnants without visible segmentation, differentiation or axis

embryo/egg not developed/not fertilized

larval musculature irregular

larva number hatched none (< 10 %)


ovary without detectable defects

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

eclosion not fulfilled (died as pupa)

larva lethality

larva bent

abdomen everted (inside-out)

larva everted (inside-out)

adult lethality


ovary without detectable defects

egg production: number <50% of expected

pupal molt not completely molted

pupal molt delayed (still prepupa)

larva lethality

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality late (L7)

pupal molt not completely molted

pupal molt not fulfilled (died with pupal and larval features)

pupal molt delayed (still larva)

larva lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


larva lethality


adult lethality

ovary without detectable defects

egg production: number <50% of expected

larva lethality

larva number hatched none (< 10 %)


adult lethality

larva lethality


fertility after larval injection decreased (no living offspring)

adult lethality

larva lethality

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

larval musculature irregular

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)


adult lethality

larva lethality

adult lethality


adult lethality

ovary without detectable defects

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

wings slightly size shortened

larva lethality

ovary without detectable defects

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

egg production: number <50% of expected


adult lethality

elytra colour lighter

elytra not closed

eclosion not fulfilled (died as pupa)

elytra partially blistered

larva lethality

embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg not developed/not fertilized

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


larva lethality

larva cuticle dorsal not closed

abdomen complete not present

metathorax not present

legs podomer potentially fused

legs podomer shortened

head appendages random not present

head appendages shape irregular

head appendages orientation irregular

appendages partially random remaining

cuticle remnants with some identifiable segments

abdomen segment number decreased

thorax partially not present

head capsule size decreased

head & thorax & abdomen partially irregular

cuticle everted (inside-out)

cuticle remnants with unidentifyable segments

larval musculature irregular

larva number few (< 50 %) hatched

eclosion not fulfilled (with pupal and adult features)


larva lethality

larva number hatched many (ca. 70-100 %)


adult lethality

head capsule not present

abdomen segment number decreased

thorax not present

head appendages random not present

head & thorax & abdomen mostly not present

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larval musculature irregular

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


eclosion not fulfilled (died as pupa)

adult lethality

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality


previtellogenic egg chamber stage sequence irregular

mature egg not present

vitellogenic egg chamber not present

egg production: number <50% of expected

eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

larva lethality

egg chamber stage sequence irregular

egg chamber shape rounded

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

previtellogenic egg chamber shape irregular

previtellogenic egg chamber orientation irregular

previtellogenic egg chamber number decreased

mature egg not present

egg production: number no/almost no eggs

adult lethality


mature egg not present

vitellogenic egg chamber not present

egg production: number <50% of expected

eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

larva lethality

egg chamber stage sequence irregular

egg chamber shape rounded

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

mature egg not present

adult lethality


pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

larva lethality

adult lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


adult lethality


larva lethality

tropharium structure irregular

tropharium shape irregular

vitellarium not present

tropharium size shortened

egg chamber not present

adult lethality

adult nutritional state bad (cachectic)

eclosion delayed (still pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

vitellogenic egg chamber sometimes rounded

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


eclosion not fulfilled (died as pupa)

pupal molt not fulfilled (died as prepupa)

legs partially necrotic

hindwing necrotic

maxillary palp orientation irregular

labial palp orientation irregular

abdomen gin-traps shortened

legs shape irregular

female genital lobes rounded

elytra not closed

antenna orientation irregular

pupal molt delayed (still prepupa)

affected eggs/embryos/larvae

vitellarium shortened

larva number hatched none (< 10 %)

adult lethality


eclosion not fulfilled (died as pupa)

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality late (L7)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

longitudinal dorsal thoracic muscles fluorescence decreased

oblique dorsal thoracic muscles thinner

the outer oblique muscles are thinner, not the median ones

oblique dorsal thoracic muscles median size shortened

abdomen bristle size shortened

abdomen gin-trap setae size shortened

wings not closed

head bristle size shortened

pupal molt delayed (still larva)

adult lethality

adult nutritional state potentially bad (cachectic)


adult lethality

ovary without detectable defects

egg production: number <50% of expected

adult nutritional state bad (cachectic)

eclosion delayed (still pupa)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality


eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality


larva lethality

adult lethality

legs tibiotarsus potentially fused

legs tibiotarsus shortened

legs femur shortened

affected eggs/embryos/larvae

leg musculature mostly not present

larval musculature partially not present

larval musculature complete not present

maybe dead

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

hindwing necrotic

elytra surface irregular

elytra not closed

larval musculature fluorescence decreased

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

eclosion not fulfilled (with pupal and adult features)


adult lethality

elytra shortened

elytra not closed

elytra shortened

previtellogenic egg chamber anterior not present

larva number hatched many (ca. 70-100 %)

egg production: number <50% of expected

egg production: number <50% of expected


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

adult lethality


adult lethality

adult nutritional state bad (cachectic)

elytra surface irregular

hindwing folding irregular

eclosion not fulfilled (died as pupa)

elytra not closed

female genital lobes rounded

oblique dorsal thoracic muscles thinner

larva lethality

adult lethality

larval musculature pattern irregular

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


larva lethality


ovary without detectable defects

egg production: number <50% of expected

adult lethality

adult lethality

leg musculature partially not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


pupal molt delayed (still larva)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult nutritional state bad (cachectic)

adult lethality


eclosion delayed (still pupa)

adult complete colour lighter

head appendages partially shape irregular

legs segment shape irregular

abdomen segment number decreased

thorax segment number decreased

head appendages partially not present

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

head capsule lateral decreased

just on one side

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

adult lethality


adult lethality

eclosion not fulfilled (not completely eclosed)

larva lethality

adult lethality


fertility after larval injection decreased (no living offspring)

eclosion delayed (still pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

adult lethality

legs tibiotarsus fused

legs femur fused

legs coxa size decreased

abdomen segment number decreased

legs size decreased

labrum bent

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched

adult lethality


larva lethality

adult lethality

larva number few (< 50 %) hatched

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

adult lethality

egg production: number no/almost no eggs

adult lethality


adult lethality

prosternal process broader

affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

mature egg deposition 4 5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

adult lethality


larva lethality

thorax segment ventral size decreased

thorax segment shape irregular

affected eggs/embryos/larvae

probably dying larva

larval musculature complete not present

dead larva

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


larva lethality

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

adult lethality


larva lethality

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult lethality


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

adult nutritional state bad (cachectic)

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

adult lethality

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult nutritional state bad (cachectic)

adult lethality


ovary without detectable defects

egg production: number <50% of expected

adult cuticularisation darker

pupa cuticularisation darker

larva lethality

abdomen segment number decreased

larva segment random number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult cuticle darker

adult lethality


ovary without detectable defects

mature egg deposition 4 >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

larva lethality

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

larva lethality

mandible not present

antenna not present

abdomen segment number decreased

legs shape irregular

head capsule not present

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

ovary without detectable defects

mature egg not present

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

antenna scapus thinner

antenna scapus bent

legs tibiotarsus size decreased

antenna proximal shape irregular

larva segment partially fused

affected eggs/embryos/larvae

larval musculature partially not present

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

eclosion not fulfilled (with pupal and adult features)


larva lethality

affected eggs/embryos/larvae

larval musculature partially fluorescence decreased

larval musculature thinner

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

larva lethality

larva number hatched many (ca. 70-100 %)

adult lethality

larva number few (< 50 %) hatched