embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva number hatched many (ca. 70-100 %)

adult lethality


head & thorax & abdomen appendages random shape irregular

head & thorax & abdomen segment random not present

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature random fluorescence not present

larval musculature random pattern irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

half ovary degenerated

vitellogenic egg chamber degenerated

lateral oviduct degenerated

mature egg not present

previtellogenic egg chamber number decreased

egg production: number no/almost no eggs


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality


affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality


affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


abdominal odoriferous glands content darker

odoriferous glands colour darker

abdominal odoriferous glands colour darker

ovary without detectable defects


embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae


head & thorax & abdomen appendages partially random not present

head & thorax & abdomen appendages shape irregular

head & thorax & abdomen segment shape irregular

There is something whitish stuff inside the empty eggs.

affected eggs/embryos/larvae

larval musculature thinner

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

adult lethality

adult lethality


head capsule not present

head & thorax segment not present

affected eggs/embryos/larvae

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva number hatched many (ca. 70-100 %)


adult lethality

adult lethality


adult lethality

adult lethality

adult nutritional state bad (cachectic)


lateral oviduct degenerated

In two case (ovary), randomly either vitellogenic or previtellogenic late egg chamber of different ovarioles are not present .

previtellogenic egg chamber late not present


abdominal odoriferous glands content decreased

cuticle remnants with unidentifyable segments

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

abdominal odoriferous glands potentially content not present

larval musculature random not present

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

egg production: number no/almost no eggs

adult lethality


cuticle remnants with some identifiable segments

head & thorax & abdomen appendages random shape irregular

head & thorax & abdomen segment random not present

affected eggs/embryos/larvae

larval musculature fluorescence decreased

larval musculature random pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

abdominal odoriferous glands content not present

abdominal odoriferous glands anterior content darker

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larval musculature not present

larva structure irregular

larva partially dorsal not closed

abdomen potentially shape swollen

larva partially everted (inside-out)

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


vitellarium potentially degenerated

vitellarium size decreased

mature egg not present

vitellogenic egg chamber degenerated

previtellogenic egg chamber not present


larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality


eclosion not fulfilled (with pupal and adult features)


head & thorax & abdomen appendages shape irregular

affected eggs/embryos/larvae

affected eggs/embryos/larvae

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


thorax & abdomen & terminus appendages random shape irregular

thorax & abdomen & terminus segment random not present

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae


legs tibiotarsus shape irregular

abdominal odoriferous glands content decreased

legs femur distal fused

legs size shortened

antenna antennal flagellum not present

affected eggs/embryos/larvae

abdominal odoriferous glands potentially content not present

adult lethality

larval musculature symmetry

larval musculature thinner

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


posterior terminus partially not present

abdomen cuticle dorsal central structure not present

legs swollen

head appendages swollen

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

adult nutritional state bad (cachectic)

egg production: number no/almost no eggs

adult lethality


adult nutritional state potentially bad (cachectic)

adult lethality

adult lethality

adult nutritional state bad (cachectic)


adult lethality

adult lethality


antenna antennal flagellum not present

maxilla swollen

legs tibiotarsus proximal fused

legs femur distal fused

legs pretarsus shape irregular

abdomen shape irregular

legs size shortened

labium swollen

affected eggs/embryos/larvae

vitellogenic egg chamber late size increased

vitellogenic egg chamber late not present

ovary without detectable defects

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected


antenna antennal flagellum not present

One of the antennal flagellum is absent in most larvae.

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae


adult lethality

adult nutritional state bad (cachectic)

adult lethality

adult lethality


head & thorax & abdomen segment random not present

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

eclosion not fulfilled (with pupal and adult features)


previtellogenic egg chamber size decreased

yolk deposition degenerated

adult lethality

ovariole degenerated

adult lethality


abdominal odoriferous glands position spotted

abdominal odoriferous glands anterior content darker

prothoracic odoriferous glands potentially colour darker

abdominal odoriferous glands colour darker

larva number hatched many (ca. 70-100 %)


lateral oviduct degenerated

vitellarium potentially degenerated

egg chamber mostly not present


head & thorax & abdomen appendages random shape irregular

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

larval musculature random pattern irregular

affected eggs/embryos/larvae


adult lethality

adult lethality

egg production: number no/almost no eggs

adult lethality


adult lethality

vitellarium size shortened

previtellogenic egg chamber number decreased

egg chamber stage sequence aborted

adult nutritional state bad (cachectic)

adult lethality

adult lethality


adult lethality


larva partially everted (inside-out)

larva everted (inside-out)

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)

adult lethality


cuticle remnants strongly fragmented (cuticle crumbs)

some wildtype larvae present. n=4

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)


adult lethality

eclosion not fulfilled (died as pupa)


adult lethality

adult lethality

adult lethality

adult lethality


adult nutritional state bad (cachectic)

adult lethality

adult lethality


adult lethality

adult lethality


larval musculature sometimes orientation irregular

leg musculature irregular

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

adult nutritional state bad (cachectic)

adult lethality

adult lethality


gena triplet bristle sometimes duplicated

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched

adult lethality

eclosion not fulfilled (with pupal and adult features)


larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult lethality

adult lethality


larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


legs femur potentially fused

legs tibiotarsus potentially fused

abdomen segment shape irregular

abdomen segmentation/division irregular

legs size decreased

affected eggs/embryos/larvae

adult lethality

larval musculature fluorescence decreased

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

adult lethality


adult lethality

adult lethality immediately after eclosion


adult lethality

larval musculature shape irregular

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

adult lethality


legs femur potentially fused

legs tibiotarsus potentially fused

legs structure irregular

head structure irregular

abdomen cuticle structure irregular

prothorax segment partially not present

head capsule size decreased

head & thorax segmentation/division irregular

affected eggs/embryos/larvae

previtellogenic egg chamber potentially number decreased

previtellogenic egg chamber position irregular

tropharium structure irregular

mature egg not present

ovary robustness decreased

dissecting the ovaries is almost impossible. Very fragile

larva number hatched none (< 10 %)


adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

larval musculature thinner

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)

adult lethality


antenna orientation irregular

labrum split

labrum potentially duplicated

affected eggs/embryos/larvae

adult lethality


adult lethality

egg chamber structure irregular

egg chamber position irregular

vitellogenic egg chamber late not present

egg chamber number decreased

mature egg not present

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality

adult lethality


larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality


embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)

adult lethality


adult lethality

larval musculature lateral thinner

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

adult lethality


adult lethality

adult lethality


adult nutritional state bad (cachectic)

adult nutritional state potentially bad (cachectic)

adult lethality

adult lethality


adult lethality

adult lethality

adult lethality


legs pretarsus shortened

legs pretarsus shortened

legs swollen

head appendages swollen

posterior terminus structure irregular

legs size decreased

head appendages size decreased

larva appendages shortened

affected eggs/embryos/larvae

adult lethality

adult lethality


adult lethality


egg chamber potentially fused

egg chamber early mostly not present

egg chamber number increased

vitellogenic egg chamber late potentially degenerated

previtellogenic egg chamber late orientation irregular

larva number hatched none (< 10 %)


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (died as pupa)


legs size decreased

head appendages shape irregular

labrum orientation irregular

mandible shape irregular

antenna shape irregular

head appendages random not present

legs podomer sometimes fused

head capsule size decreased

affected eggs/embryos/larvae

adult lethality

affected eggs/embryos/larvae

adult lethality


eclosion not fulfilled (died as pupa)


legs structure irregular

abdomen cuticle ectopically structure present

legs potentially number increased

antenna antennal flagellum shortened

affected eggs/embryos/larvae

larval musculature potentially thinner

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


thorax segment potentially number decreased

head appendages shape irregular

larva appendages swollen

abdomen not present

head appendages swollen

larva segmentation/division irregular

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality

eclosion not fulfilled (with pupal and adult features)


larval musculature fluorescence decreased

affected eggs/embryos/larvae

adult lethality


affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


labrum not present

labrum size decreased

antenna structure irregular

antenna not present

affected eggs/embryos/larvae

adult lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


gena triplet gena triplet dorsal number increased

labrum size decreased

labrum not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


abdomen segment number decreased

head mostly not present

posterior terminus not present

labrum position irregular

labrum size decreased

head & thorax partially not present

maxilla potentially fused

labium potentially fused

head appendages random not present

head capsule shape irregular

larva size decreased

abdomen segment shape irregular

head capsule size decreased

larva dorsal bent

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

larva number hatched none (< 10 %)

adult lethality

egg production: number <50% of expected

adult lethality


labium

legs shape irregular

legs tibiotarsus potentially fused

legs femur potentially fused

legs size decreased

mandible not present

labrum not present

antenna not present

head capsule size decreased

head capsule mostly not present

posterior terminus not present

metathorax not present

abdomen segment number decreased

thorax posterior constricted

larva constricted

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality

adult lethality

adult lethality


legs sometimes size decreased

legs tibiotarsus sometimes potentially fused

legs femur sometimes potentially fused

legs shape irregular

head structure irregular

head appendages orientation irregular

posterior terminus not present

abdomen segment number decreased

head capsule size decreased

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

adult lethality

larva number hatched many (ca. 70-100 %)

adult lethality


cuticle remnants strongly fragmented (cuticle crumbs)

abdomen posterior segmentation/division irregular

abdomen segment number decreased

head mostly not present

abdomen not present

thorax segment number decreased

head not present

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality


adult lethality


antenna structure irregular

antenna not present

thorax anterior partially not present

head capsule size decreased

affected eggs/embryos/larvae

adult lethality

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


legs sometimes structure irregular

head appendages random not present

head not present

head & thorax mostly not present

thorax anterior partially not present

head structure irregular

larva dorsal bent

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


larva cuticle dorsal potentially not fused

head appendages orientation irregular

larva everted (inside-out)

legs podomer sometimes potentially fused

legs potentially size decreased

larva cuticle dorsal irregular

abdomen segment number decreased

thorax partially not present

head capsule size decreased

larva dorsal bent

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

adult lethality


adult lethality

adult lethality


affected eggs/embryos/larvae

adult lethality

adult lethality


abdominal odoriferous glands content opaque

adult lethality

adult lethality

adult lethality immediately after eclosion


abdomen cuticle irregular

thorax unilateral mostly not present

head unilateral mostly not present

head & thorax unilateral incomplete

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

abdominal odoriferous glands content irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


abdomen cuticle irregular

thorax unilateral mostly not present

head unilateral mostly not present

head & thorax unilateral incomplete

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


larva random shape irregular

head & thorax & abdomen segment random shape irregular

head & thorax & abdomen appendages random shape irregular

head & thorax & abdomen appendages random not present

head & thorax & abdomen segment random not present

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality


adult lethality

egg production: number no/almost no eggs

adult lethality


head & thorax & abdomen segment not present

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


adult lethality

larva number hatched many (ca. 70-100 %)


affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult nutritional state bad (cachectic)

adult nutritional state potentially bad (cachectic)

eclosion not fulfilled (with pupal and adult features)


abdominal odoriferous glands content decreased

abdominal odoriferous glands anterior content darker

abdominal odoriferous glands content not present


gena triplet position irregular

legs tibiotarsus shape irregular

gena triplet number increased

affected eggs/embryos/larvae

larval musculature pattern irregular

larval musculature thinner

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

vitellarium size decreased

egg chamber not present

vitellogenic egg chamber not present

previtellogenic egg chamber not present

mature egg not present

ovary degenerated

adult lethality


adult lethality


abdomen random number decreased

head appendages partially random not present

larva dorsal pattern irregular

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature random not present

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

egg chamber stage sequence irregular

vitellogenic egg chamber late degenerated

previtellogenic egg chamber early shape irregular

mature egg not present


adult lethality

adult nutritional state bad (cachectic)

adult lethality

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae


head & thorax & abdomen segment random not present

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

adult lethality


ovary without detectable defects

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:


abdominal odoriferous glands content necrotic

abdominal odoriferous glands partially content darker

larva number hatched many (ca. 70-100 %)


adult lethality

adult nutritional state bad (cachectic)

adult lethality


adult lethality

adult lethality

adult lethality


head appendages shape irregular

affected eggs/embryos/larvae

larval musculature partially not present

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

eclosion not fulfilled (with pupal and adult features)


affected eggs/embryos/larvae

larva number few (< 50 %) hatched


larva potentially everted (inside-out)

larva partially posterior everted (inside-out)

larva complete everted (inside-out)

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larval musculature pattern irregular

affected eggs/embryos/larvae


larva number few (< 50 %) hatched

adult lethality


embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larval musculature pattern irregular

affected eggs/embryos/larvae


adult lethality

previtellogenic egg chamber number decreased

lateral oviduct degenerated

mature egg not fused

vitellarium size decreased

vitellogenic egg chamber degenerated

previtellogenic egg chamber not present

adult nutritional state bad (cachectic)


adult lethality


head appendages shape irregular

legs shape irregular

gena triplet number increased

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult nutritional state bad (cachectic)


labium not present

head appendages random position irregular

larva dorsal not closed

abdomen segment number decreased

prothorax segment not present

head appendages shape irregular

affected eggs/embryos/larvae

head musculature pattern irregular

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


larva constricted

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

abdominal odoriferous glands content not present

abdominal odoriferous glands colour darker

odoriferous glands necrotic

odoriferous glands darker

adult lethality

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality

adult nutritional state bad (cachectic)

egg production: number no/almost no eggs


vitellarium size decreased

lateral oviduct necrotic

vitellogenic egg chamber degenerated

mature egg not present

previtellogenic egg chamber not present

adult lethality


head & thorax & abdomen segment random not present

cuticle remnants strongly fragmented (cuticle crumbs)

affected eggs/embryos/larvae

larval musculature random not present

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

larval musculature random not present

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


larva number hatched many (ca. 70-100 %)


affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg

affected eggs/embryos/larvae

adult lethality


larva shape irregular

larva bent

larva partially everted (inside-out)

abdomen segment number decreased

larva complete everted (inside-out)

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


head & thorax & abdomen appendages random shape irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


eclosion not fulfilled (with pupal and adult features)


cuticle remnants

affected eggs/embryos/larvae

adult lethality


affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult nutritional state potentially bad (cachectic)


adult lethality

adult lethality


adult lethality


adult lethality

egg production: number <50% of expected

adult lethality


larval musculature fluorescence decreased

affected eggs/embryos/larvae

yolk sometimes amount decreased

adult lethality

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected


adult lethality

adult lethality

adult lethality immediately after eclosion


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

previtellogenic egg chamber sometimes not present

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (died as pupa)


adult lethality

adult lethality

adult lethality


vitellogenic egg chamber not present

vitellogenic egg chamber number decreased

previtellogenic egg chamber not present

mature egg not present

egg production: number no/almost no eggs

adult lethality

eclosion not fulfilled (died as pupa)


adult lethality

egg chamber mostly not present

mature egg not present

only undefineable yolk remaining in oviduct


affected eggs/embryos/larvae

adult lethality

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

eclosion not fulfilled (died as pupa)


larva segment number decreased

larva rounded

legs sometimes fused

larva circumference decreased

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


legs sometimes size decreased

legs segment sometimes number decreased

affected eggs/embryos/larvae

adult lethality

adult lethality


adult lethality

adult lethality

adult lethality

adult lethality


abdomen tracheal opening size increased

mesothoracic tracheal opening size increased

thorax & abdomen circumference decreased

head appendages position irregular

legs segment potentially number decreased

abdomen partially dorsal not fused

legs size decreased

head capsule size decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

follicular epithelium potentially partially amount increased

vitellogenic egg chamber potentially degenerated

vitellogenic egg chamber partially position irregular

tropharium structure irregular

previtellogenic egg chamber not present

mature egg potentially not present

larva number few (< 50 %) hatched

egg production: number <50% of expected

eclosion not fulfilled (with pupal and adult features)


adult lethality

adult nutritional state bad (cachectic)

eclosion not fulfilled (died as pupa)


adult lethality

larva number hatched many (ca. 70-100 %)

adult lethality


eclosion not fulfilled (died as pupa)


affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

adult lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (died as pupa)


cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


larva cuticle dorsal not fused

legs size decreased

larva segment number decreased

abdomen segment number decreased

thorax segment number decreased

head appendages number decreased

larva everted (inside-out)

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult lethality


adult lethality


vitellogenic egg chamber potentially sometimes degenerated

vitellogenic egg chamber potentially sometimes fused

egg chamber number decreased

yolk late amount decreased

mature egg not present

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

previtellogenic egg chamber not present

mature egg not present

egg chamber mostly not present


head & thorax & abdomen segment shape irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

abdominal odoriferous glands potentially content darker

adult lethality

embryo/egg not developed/not fertilized

egg size decreased

affected eggs/embryos/larvae

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

adult lethality


larva number hatched none (< 10 %)


larva number few (< 50 %) hatched

abdomen segment potentially partially pattern fused

abdomen segment pattern irregular

affected eggs/embryos/larvae


affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva number hatched none (< 10 %)

affected eggs/embryos/larvae


many (ca. 70-100 %)


larva number few (< 50 %) hatched


larva number hatched none (< 10 %)

egg production: number no/almost no eggs


egg production: number <50% of expected


head & thorax & abdomen segment partially not present

head mostly not present

head & thorax partially not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


labrum position irregular

head appendages random not present

labium sometimes not present

maxilla sometimes not present

mandible not present

labrum not present

antenna not present

head appendages position irregular

prothoracic leg segment not present

head capsule not present

larva everted (inside-out)

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva anterior everted (inside-out)

larva anterior circumference decreased

head & thorax segment everted (inside-out)

larva complete everted (inside-out)

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality


head & thorax & abdomen segment random not present

affected eggs/embryos/larvae

abdominal odoriferous glands content not present

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

eclosion not fulfilled (with pupal and adult features)

larva number hatched many (ca. 70-100 %)


cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

adult lethality


abdominal odoriferous glands content darker


head appendages position irregular

head & thorax segment random not present

head appendages sometimes random not present

head & thorax & abdomen segment shape irregular

cuticle remnants with some identifiable segments

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


abdomen segment posterior potentially everted (inside-out)

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

adult lethality

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


head & thorax & abdomen segment potentially shape irregular

affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

adult lethality


larval musculature pattern irregular

affected eggs/embryos/larvae


adult lethality

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number no/almost no eggs


head & thorax & abdomen segment random not present

affected eggs/embryos/larvae

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

embryo/egg with embryonic tissue, no cuticle, no fluorescence

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

larva number hatched many (ca. 70-100 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


head & thorax & abdomen segment random potentially not present

head appendages random not present

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched

adult lethality


prothoracic leg not present

prothorax segment not present

thorax & abdomen segment number decreased

larva complete potentially everted (inside-out)

prothoracic leg potentially everted (inside-out)

abdomen segment number decreased

head appendages position irregular

labrum potentially not present

cuticle remnants with some identifiable segments

abdomen shape irregular

legs shape irregular

head capsule not present

head & thorax segment constricted

Constriction often occours between head and thorax segment.

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

abdominal odoriferous glands content darker

abdominal odoriferous glands content not present

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality


adult lethality

adult lethality

larva number hatched none (< 10 %)


head appendages random not present

head appendages shape irregular

abdomen segment number decreased

abdomen posterior ventral constricted

abdomen posterior potentially everted (inside-out)

legs potentially size shortened

head capsule not present

head & thorax & abdomen segment shape irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


head appendages position irregular

head & thorax segment random not present

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


lateral oviduct degenerated

vitellarium potentially size decreased

tropharium pattern irregular

previtellogenic egg chamber not present

vitellogenic egg chamber potentially degenerated

mature egg mostly not present

egg production: number no/almost no eggs


larva number hatched many (ca. 70-100 %)


affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

eclosion not fulfilled (with pupal and adult features)


head capsule not present

head appendages random not present

head & thorax & abdomen segment random not present

legs shape irregular

head appendages position irregular

larva dorsal not closed

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

egg structure irregular

abdominal odoriferous glands content decreased

larval musculature random not present

embryo/egg not developed/not fertilized

egg potentially size decreased

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

adult lethality


abdomen bristle pattern irregular

head capsule not present

abdomen segment posterior everted (inside-out)

abdomen bristle potentially everted (inside-out)

thorax segment constricted

head & thorax & abdomen appendages shape irregular

abdomen segment posterior dorsal bent

legs segment fused

abdomen segment posterior constricted

legs random size decreased

head appendages random not present

head & thorax & abdomen appendages random not present

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


cuticle remnants with some identifiable segments

head & thorax & abdomen segment shape irregular

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)


larva fragmented

abdomen segment constricted

abdomen segment number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

adult lethality

egg production: number no/almost no eggs

adult lethality

adult lethality


adult lethality

adult nutritional state bad (cachectic)

adult lethality

adult lethality


adult lethality


affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)


legs shape irregular

head appendages position irregular

affected eggs/embryos/larvae

adult lethality

adult lethality


affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

eclosion not fulfilled (with pupal and adult features)


labrum not present

antenna bent

legs segment potentially fused

antenna antennal flagellum not present

larva shape irregular

affected eggs/embryos/larvae

larval musculature random not present

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


vertex vertex triplet posterior potentially not present

legs shape irregular

abdomen segment number decreased

antenna antennal flagellum not present

head & thorax & abdomen segment random not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


larva dorsal not closed

abdomen segment number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched


adult lethality

adult lethality

adult nutritional state bad (cachectic)

adult lethality


adult lethality

adult lethality


labrum shape irregular

labium shape irregular

head capsule size decreased

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

larval musculature fluorescence decreased

larval musculature pattern irregular

affected eggs/embryos/larvae

ovary without detectable defects

adult nutritional state bad (cachectic)

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality

adult lethality immediately after eclosion


adult lethality

adult lethality

adult lethality


legs podomer sometimes structure irregular

thorax partially not present

labrum size decreased

labrum not present

antenna not present

mandible not present

labium not present

maxilla not present

head capsule need term partially not present

abdomen segment number decreased

thorax partially not present

head partially not present

head & thorax not present

cuticle remnants with unidentifyable segments

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


legs tibiotarsus random potentially fused

legs femur random potentially fused

posterior terminus structure irregular

abdomen cuticle shape irregular

legs size decreased

affected eggs/embryos/larvae

adult lethality

adult lethality


head appendages swollen

legs partially swollen

abdomen segment number decreased

legs shape irregular

antenna structure irregular

larva constricted

cuticle remnants without visible segmentation but ap-polarity

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality


adult lethality

adult nutritional state potentially bad (cachectic)

adult nutritional state bad (cachectic)

egg production: number no/almost no eggs

adult lethality

adult lethality


legs sometimes random structure not present

thorax segmentation/division irregular

thorax not present

mesothorax not present

metathorax not present

thorax segment number decreased

legs podomer potentially fused

legs size decreased

abdomen segment number decreased

legs structure irregular

head capsule shape irregular

thorax & abdomen segment mostly not present

head remaining

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


adult lethality

vitellarium potentially size decreased

egg chamber late number decreased

mature egg not present

adult lethality


adult lethality

adult nutritional state bad (cachectic)

adult lethality


labrum not present

labrum slightly size decreased

affected eggs/embryos/larvae

larva number hatched many (ca. 70-100 %)

eclosion not fulfilled (with pupal and adult features)


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

previtellogenic egg chamber number increased

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected


affected eggs/embryos/larvae

previtellogenic egg chamber potentially number increased

mature egg not present

adult lethality


larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


abdomen cuticle structure irregular

larva partially everted (inside-out)

affected eggs/embryos/larvae

adult lethality

larval musculature potentially thinner

affected eggs/embryos/larvae

adult lethality

adult lethality

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


posterior terminus not present

abdomen segment number decreased

legs size decreased

antenna potentially slightly size decreased

affected eggs/embryos/larvae

adult lethality

larval musculature mostly degenerated

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality


legs femur potentially sometimes fused

legs sometimes shape irregular

antenna not present

abdomen not present

thorax partially not present

head mostly not present

head & thorax partially not present

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

adult lethality

adult lethality


larva potentially everted (inside-out)

larva potentially partially everted (inside-out)

cuticle remnants strongly fragmented (cuticle crumbs)

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality

adult lethality


head capsule shape irregular

head appendages shape irregular

labrum sometimes potentially not present

head structure split

head position irregular

head structure irregular

prothorax mostly not present

legs shape irregular

posterior terminus structure irregular

abdomen segment number decreased

legs potentially size decreased

head not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

ovary without detectable defects

adult lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


ovary without detectable defects

mature egg deposition 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


ovary without detectable defects

previtellogenic egg chamber position irregular

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


legs structure irregular

labrum shape irregular

antenna not present

legs pretarsus not present

antenna unilateral not present

affected eggs/embryos/larvae

adult lethality


larva partially everted (inside-out)

affected eggs/embryos/larvae

adult lethality

adult lethality


abdominal odoriferous glands colour opaque

abdominal odoriferous glands content split

adult lethality

larva number hatched many (ca. 70-100 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

adult lethality

egg production: number no/almost no eggs

adult lethality

adult lethality


cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

adult lethality


affected eggs/embryos/larvae

adult lethality


adult lethality

adult lethality

adult lethality

adult lethality

eclosion not fulfilled (died as pupa)


adult lethality


affected eggs/embryos/larvae

larva number few (< 50 %) hatched

head appendages partially not present

abdomen segment partially split

posterior terminus not present

abdomen segment number decreased

abdomen shape irregular

head appendages shape irregular

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

larval musculature thinner

larval musculature fluorescence decreased

larval musculature pattern irregular


adult lethality

adult lethality


abdominal odoriferous glands content necrotic

abdominal odoriferous glands content decreased

abdominal odoriferous glands partially content darker

abdominal odoriferous glands colour darker


adult lethality

adult nutritional state potentially bad (cachectic)

adult lethality

adult nutritional state bad (cachectic)


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


head & thorax appendages random shape irregular

head & thorax & abdomen appendages random not present

head & thorax & abdomen segment random not present

affected eggs/embryos/larvae

larval musculature random pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


larval musculature potentially pattern irregular

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


larva number hatched many (ca. 70-100 %)


cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

larval musculature random not present

embryo/egg with embryonic tissue, no cuticle, no fluorescence

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality

eclosion not fulfilled (with pupal and adult features)


egg production: number no/almost no eggs

adult lethality


head & thorax & abdomen appendages shape irregular

affected eggs/embryos/larvae

larval musculature random not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


head & thorax & abdomen appendages random shape irregular

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


head capsule posterior potentially structure irregular

head capsule posterior potentially constricted

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


labrum not present

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

abdominal odoriferous glands potentially content not present

abdominal odoriferous glands content darker

abdominal odoriferous glands content necrotic

odoriferous glands colour darker

larval musculature random pattern irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


head capsule not present

head appendages random position irregular

head appendages random shape irregular

head & thorax & abdomen segment random not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

adult nutritional state bad (cachectic)

adult lethality

adult lethality


lateral oviduct necrotic

vitellarium potentially size increased

vitellogenic egg chamber degenerated

vitellarium size decreased

previtellogenic egg chamber not present


embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

larva number hatched many (ca. 70-100 %)


affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, no fluorescence

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

eclosion not fulfilled (with pupal and adult features)


adult lethality


adult lethality

ovary without detectable defects

lateral oviduct potentially late degenerated

vitellogenic egg chamber potentially late shape broader

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number no/almost no eggs


adult lethality

labium potentially posterior structure swollen

affected eggs/embryos/larvae

adult nutritional state bad (cachectic)

ovary without detectable defects

adult lethality

adult nutritional state potentially bad (cachectic)

egg production: number <50% of expected

egg production: number <50% of expected


adult lethality

adult lethality


head & thorax appendages shape irregular

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


cuticle remnants with unidentifyable segments

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

larval musculature random pattern irregular

larval musculature random not present

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


head & thorax & abdomen segment random not present

affected eggs/embryos/larvae

larval musculature random not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva number hatched many (ca. 70-100 %)

adult lethality


larval musculature thinner

larval musculature fluorescence decreased

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


antenna not present

head appendages partially not present

head capsule not present

head appendages orientation irregular

head appendages shape irregular

labrum position irregular

labrum partially not present

labrum not present

head & thorax segment random not present

affected eggs/embryos/larvae

leg musculature potentially mostly not present

larval musculature random not present

head musculature not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


eclosion not fulfilled (with pupal and adult features)


abdominal odoriferous glands potentially content not present

abdominal odoriferous glands anterior content darker


adult lethality

ovariole size decreased

vitellogenic egg chamber

vitellogenic egg chamber degenerated

mature egg not present

previtellogenic egg chamber not present

adult nutritional state bad (cachectic)

egg production: number no/almost no eggs


adult lethality

lateral oviduct degenerated

previtellogenic egg chamber number decreased

vitellogenic egg chamber degenerated

mature egg not present

vitellarium size decreased

previtellogenic egg chamber not present


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

ovariole size shortened

vitellarium not present

egg chamber not present

mature egg not present

lateral oviduct degenerated

adult lethality


head & thorax & abdomen appendages random shape irregular

larva dorsal partially not closed

head & thorax & abdomen appendages random not present

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

adult lethality


labrum potentially size decreased

head & thorax appendages potentially size shortened

pygopodium partially size irregular

legs partially size decreased

head appendages shape swollen

head & thorax appendages shape swollen

affected eggs/embryos/larvae

leg musculature pattern irregular

leg musculature fluorescence decreased

leg musculature random not present

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

lateral oviduct degenerated

mature egg

vitellogenic egg chamber degenerated


adult lethality

adult lethality


abdomen segment posterior dorsal bent

larva appendages shape irregular

abdomen segment potentially number decreased

larva segment shape irregular

abdomen segment thinner

larva partially everted (inside-out)

cuticle remnants with some identifiable segments

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

adult lethality

adult lethality


adult lethality


cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva number hatched many (ca. 70-100 %)


head & thorax & abdomen segment random not present

affected eggs/embryos/larvae

larval musculature random not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

eclosion not fulfilled (with pupal and adult features)


larval musculature dorsoventral-axis constricted

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

vitellarium degenerated

vitellarium size increased

mature egg not present

adult lethality

adult nutritional state bad (cachectic)


larva number few (< 50 %) hatched


adult lethality

adult nutritional state potentially bad (cachectic)

egg production: number no/almost no eggs


affected eggs/embryos/larvae

larval musculature random pattern irregular

larval musculature fluorescence decreased

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

prothoracic odoriferous glands colour darker

abdominal odoriferous glands colour darker

adult lethality

adult lethality

adult lethality


adult lethality

adult nutritional state bad (cachectic)

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


abdominal odoriferous glands content not present

abdominal odoriferous glands not present

prothoracic odoriferous glands potentially not present

head appendages shape irregular

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


head & thorax & abdomen appendages random partially not present

labrum size shortened

head appendages random partially shape irregular

head appendages random partially not present

head & thorax & abdomen segment random partially not present

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


larva shape irregular

legs pretarsus proximal size decreased

legs pretarsus shape irregular

legs tibiotarsus distal shape irregular

legs tibiotarsus size decreased

larva dorsal partially pattern irregular

affected eggs/embryos/larvae

leg musculature potentially not present

larval musculature random not present

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality


head & thorax & abdomen segment random not present

head & thorax & abdomen segment shape irregular

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae


labium potentially posterior structure swollen

head capsule anterior partially shape broader

affected eggs/embryos/larvae

larval musculature random not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


lateral oviduct degenerated

mature egg not present

previtellogenic egg chamber degenerated

previtellogenic egg chamber not present

vitellogenic egg chamber

vitellarium degenerated

adult lethality

egg production: number no/almost no eggs


adult lethality

adult lethality

adult lethality

adult lethality


adult lethality

adult lethality

adult nutritional state bad (cachectic)

adult lethality


affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, no fluorescence

Only few eggs are present (10-12 eggs)

affected eggs/embryos/larvae

previtellogenic egg chamber not present

egg chamber mostly not present

mature egg not present

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae


affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

egg chamber not present

mature egg not present

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


larva dorsal bent

larva circumference decreased

larva partially everted (inside-out)

larva complete everted (inside-out)

thorax & abdomen segment random not present

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality

adult nutritional state bad (cachectic)


vitellarium size decreased

egg chamber not present

mature egg not present

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality


legs shape irregular

head capsule not present

cuticle remnants with unidentifyable segments

abdomen segment potentially number decreased

thorax segment not present

head appendages random not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva number hatched many (ca. 70-100 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

adult lethality

adult lethality


adult lethality

previtellogenic egg chamber potentially not present

ovary without detectable defects


adult lethality


adult lethality

vitellogenic egg chamber potentially degenerated

previtellogenic egg chamber not present

mature egg not present

egg chamber mostly not present

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

adult lethality


mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult nutritional state potentially bad (cachectic)

egg production: number no/almost no eggs

adult lethality

adult lethality


ovary without detectable defects

adult lethality


head appendages position irregular

larva partially potentially everted (inside-out)

head & thorax & abdomen segment random not present

head & thorax & abdomen segment shape irregular

head capsule not present

larva everted (inside-out)

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

larval musculature potentially random pattern not present

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


abdominal odoriferous glands potentially content darker


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature potentially random not present

larval musculature potentially thinner

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


abdominal odoriferous glands content darker

tropharium degenerated

egg chamber not present

abdominal odoriferous glands content not present

mature egg not present

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva number hatched many (ca. 70-100 %)


head appendages random not present

larva not closed

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


thorax segment ventral size increased

labium split

head appendages swollen

labrum not present

abdomen segment posterior dorsal bent

abdomen segment constricted

antenna antennal flagellum potentially duplicated

head appendages position irregular

abdomen segment sometimes not present

legs shape irregular

head bristle pattern irregular

head & thorax segment potentially swollen

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


head appendages position irregular

head appendages random not present

thorax segment sometimes not present

head & thorax segment random not present

legs shape irregular

head capsule not present

larva not closed

affected eggs/embryos/larvae

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


head & thorax & abdomen segment shape irregular

legs podomer potentially number decreased

legs size decreased

head appendages swollen

head appendages position irregular

thorax & abdomen segment potentially random not present

head & thorax & abdomen segment swollen

abdomen segment posterior circumference decreased

larva potentially circumference decreased

abdomen posterior dorsal bent

legs swollen

head appendages shape irregular

larva partially everted (inside-out)

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality


larva

Molting to the 2nd instar  larva  incomplete.L2 cuticle formed but not hatched from L1 cuticle.  Both cuticles remain attached. Ventrally, there are unpaired connections  between both cuticles.

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


abdomen segment ventral not closed

abdomen segment sometimes potentially not present

abdomen bristle potentially number decreased

abdomen cuticle ventral potentially structure thinner

affected eggs/embryos/larvae

larval musculature thinner

larval musculature random fluorescence not present

larval musculature complete fluorescence not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


egg chamber potentially degenerated

previtellogenic egg chamber not present

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number no/almost no eggs

adult lethality

eclosion not fulfilled (with pupal and adult features)


posterior terminus segment potentially size decreased

legs pretarsus bent

affected eggs/embryos/larvae

larval musculature potentially rounded

larval musculature random pattern not present

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


head & thorax & abdomen segment random potentially not present

head & thorax & abdomen segment shape irregular

larva everted (inside-out)

cuticle remnants with unidentifyable segments

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality


head appendages position irregular

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


head & thorax & abdomen segment random not present

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


previtellogenic egg chamber not present

vitellarium potentially size decreased

vitellogenic egg chamber orientation irregular

egg chamber number decreased

vitellogenic egg chamber number decreased

larva number hatched none (< 10 %)

adult lethality

adult lethality


adult lethality

egg production: number <50% of expected


adult lethality

adult lethality

adult lethality


egg production: number <50% of expected

adult lethality

adult lethality

adult lethality

ovary without detectable defects


adult lethality

egg production: number <50% of expected

adult lethality

abdomen segment potentially number decreased

abdomen shape irregular

legs number decreased

head not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae


affected eggs/embryos/larvae

larval musculature shape rounded

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


prothoracic odoriferous glands partially darker

affected eggs/embryos/larvae

prothoracic odoriferous glands darker

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


eclosion not fulfilled (with pupal and adult features)

larva number hatched none (< 10 %)

egg production: number no/almost no eggs


larva number hatched many (ca. 70-100 %)


abdomen tracheal opening not present

mesothoracic tracheal opening not present

affected eggs/embryos/larvae

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected


adult lethality

adult lethality


adult lethality

adult nutritional state bad (cachectic)


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult nutritional state potentially bad (cachectic)

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (died as pupa)


larva segment number decreased

abdomen segment number decreased

metathorax constricted

abdomen segment random constricted

head capsule size decreased

thorax & abdomen segment random constricted

affected eggs/embryos/larvae

larval musculature fluorescence decreased

larval musculature pattern irregular

affected eggs/embryos/larvae

ovary without detectable defects

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected


adult lethality

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected


maxilla present

larva dorsal partially not fused

larva everted (inside-out)

antenna present

abdomen segment number decreased

thorax segment symmetry irregular

head mostly not present

larva segment number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)


adult lethality


adult lethality

adult lethality

adult nutritional state bad (cachectic)

adult lethality


abdomen segment number decreased

abdomen partially segmentation/division irregular

legs femur not present

head capsule size decreased

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched


egg production: number <50% of expected

adult lethality


adult lethality

adult lethality

larva number hatched none (< 10 %)


adult lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality

eclosion not fulfilled (died as pupa)


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

larva number hatched none (< 10 %)


adult lethality

abdomen segment sometimes number decreased

thorax segment partially number decreased

head appendages sometimes not present

larva sometimes everted (inside-out)

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


larva number hatched none (< 10 %)


adult lethality

adult lethality


vitellogenic egg chamber degenerated

previtellogenic egg chamber not present

egg chamber number decreased

larva number hatched


affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


abdominal odoriferous glands content darker

adult lethality

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

adult lethality

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched


adult lethality


head appendages position irregular

head & thorax & abdomen segment random not present

adult lethality

abdomen segment number decreased

head appendages random not present

head & thorax & abdomen segment shape irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


legs shape irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

eclosion not fulfilled (with pupal and adult features)


thorax & abdomen segment random not present

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


larva number hatched many (ca. 70-100 %)


adult lethality

ovariole potentially size shortened

vitellarium potentially size increased

egg chamber not present

mature egg not present

adult nutritional state potentially bad (cachectic)

egg production: number no/almost no eggs


adult lethality

egg chamber not present

mature egg not present


cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


legs size shortened

head & thorax & abdomen segment potentially swollen

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality


abdominal odoriferous glands content decreased

adult lethality


previtellogenic egg chamber potentially not present

tropharium potentially size decreased

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality


mandible not present

labrum not present

antenna not present

head capsule not present

head appendages random not present

labrum position irregular

head appendages position irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


thorax musculature pattern irregular

larval musculature pattern irregular

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


head & thorax & abdomen segment potentially random not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult nutritional state bad (cachectic)

adult lethality


larva complete everted (inside-out)

antenna sometimes not present

labrum potentially not present

head appendages random not present

head appendages shape irregular

head & thorax & abdomen segment random not present

thorax segment random potentially not present

head appendages position irregular

head & thorax & abdomen segment shape irregular

affected eggs/embryos/larvae

larval musculature random not present

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

eclosion not fulfilled (with pupal and adult features)


head appendages random not present

cuticle remnants with unidentifyable segments

head appendages position irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


larva number hatched many (ca. 70-100 %)

adult lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

adult lethality

egg production: number no/almost no eggs

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


larval musculature random not present

larval musculature rounded

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality


head appendages random not present

antenna sometimes not present

labrum shape irregular

labrum size decreased

head appendages position irregular

head appendages potentially swollen

head capsule not present

head & thorax segment random not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


cuticle remnants with some identifiable segments

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

abdominal odoriferous glands content darker

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


abdomen segment number decreased

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched


head capsule not present

abdomen segment number decreased

thorax segment number decreased

head appendages shape irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


previtellogenic egg chamber not present

mature egg mostly not present


affected eggs/embryos/larvae

adult lethality

egg size decreased

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


head appendages potentially swollen

head capsule not present

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


legs shape irregular

affected eggs/embryos/larvae

affected eggs/embryos/larvae

egg production: number <50% of expected

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality


larval musculature complete fluorescence not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


tropharium structure irregular

Potentially yolk deposition observed in tropharium.

vitellarium degenerated

egg chamber not present

mature egg not present

adult lethality


head & thorax & abdomen segment random sometimes not present

legs shape irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality


legs potentially swollen

legs shape irregular

gena triplet bristle pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


egg chamber potentially degenerated

egg chamber potentially not present

mature egg mostly not present

adult nutritional state bad (cachectic)

adult lethality


adult lethality

adult lethality


legs shape irregular

thorax segment random not present

larva proximal constricted

head & thorax & abdomen segment not present

larva shape irregular

affected eggs/embryos/larvae

larval musculature random pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


posterior terminus partially not present

abdomen segment number decreased

abdomen segment shape irregular

legs shape irregular

head capsule partially size decreased

head & thorax & abdomen appendages shape irregular

affected eggs/embryos/larvae

larval musculature random not present

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


head capsule not present

head appendages partially not present

head appendages shape irregular

labrum potentially not present

labrum size decreased

labrum position irregular

head & thorax & abdomen segment random not present

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched many (ca. 70-100 %)


head appendages distal structure swollen

larva appendages shape irregular

pygopodium partially not present

legs shape irregular

head appendages shape irregular

larva partially everted (inside-out)

affected eggs/embryos/larvae

larval musculature random not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

egg production: number no/almost no eggs

adult lethality


antenna not present

head & thorax & abdomen appendages random shape irregular

head capsule not present

head appendages shape irregular

labrum not present

cuticle remnants with some identifiable segments

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature random not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva number few (< 50 %) hatched


ovary without detectable defects

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:


ovary without detectable defects

adult lethality


mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality


adult nutritional state potentially bad (cachectic)

adult lethality

adult nutritional state bad (cachectic)

adult lethality

adult lethality


adult lethality

egg chamber potentially degenerated

egg chamber mostly not present

adult lethality


adult lethality


embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

ovary without detectable defects

adult lethality


In this Repetition generally less eggs have been laid. Potenntially empty eggs background effect.

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva number hatched none (< 10 %)

egg production: number <50% of expected


larva number hatched none (< 10 %)


egg production: number <50% of expected


labium sometimes potentially not present

labrum sometimes potentially not present

abdomen segment potentially partially fused

abdomen segment slightly number increased

abdomen segment partially irregular

head appendages position irregular

head & thorax appendages partially not present

affected eggs/embryos/larvae


affected eggs/embryos/larvae


affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

larva number hatched none (< 10 %)


abdomen shape irregular

legs segment partially not present

larva segment partially not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


head & thorax segment partially not present

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


larva number hatched many (ca. 70-100 %)


larva dorsal partially irregular

larva dorsal partially not closed

head & thorax appendages partially random not present

affected eggs/embryos/larvae

egg production: number <50% of expected


egg production: number


antenna mostly not present

abdomen segment number decreased

head appendages partially random not present

affected eggs/embryos/larvae

egg production: number


larva number hatched none (< 10 %)

egg production: number no/almost no eggs


head & thorax & abdomen segment partially random not present

abdomen segment number decreased

larva partially potentially everted (inside-out)

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


larva number hatched none (< 10 %)

egg production: number <50% of expected

abdominal odoriferous glands less secretion


larva number hatched none (< 10 %)

abdominal odoriferous glands size decrease


larva everted (inside-out)

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected


larva number hatched none (< 10 %)


larva partially everted (inside-out)

larva everted (inside-out)

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


head capsule size irregular

head capsule shape irregular

antenna mostly not present

head appendages partially not present

abdomen segment number decreased

head appendages partially position irregular

affected eggs/embryos/larvae


larva number hatched none (< 10 %)

egg production: number <50% of expected


larva number few (< 50 %) hatched


head & thorax appendages potentially shape irregular

larva number few (< 50 %) hatched


egg production: number <50% of expected


larva number few (< 50 %) hatched

egg production: number <50% of expected


head & thorax appendages everted (inside-out)

head & thorax appendages partially size decreased

legs partially structure irregular

antenna structure irregular

head & thorax appendages partially shape irregular

Mostly antenna and one or two legs are affected.

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


larva constricted

head appendages random partially not present

head & thorax not present

affected eggs/embryos/larvae

egg production: number <50% of expected

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected


egg production: number <50% of expected

abdominal odoriferous glands irregular reservoir size


egg production: number <50% of expected

larva number hatched many (ca. 70-100 %)


abdomen segment number decreased

head mostly not present

head & thorax mostly not present

affected eggs/embryos/larvae


larva constricted

abdomen number decreased

head & thorax mostly not present

affected eggs/embryos/larvae


abdomen potentially everted (inside-out)

head & thorax appendages shape irregular

abdomen shape irregular

head & thorax appendages swollen

affected eggs/embryos/larvae


head capsule size decreased

antenna mostly not present

head appendages random not present

affected eggs/embryos/larvae


larva number hatched none (< 10 %)


egg production: number <50% of expected

larva number few (< 50 %) hatched


egg production: number no/almost no eggs

larva number hatched none (< 10 %)


larva number hatched none (< 10 %)

egg production: number <50% of expected


antenna sometimes structure decreased

antenna sometimes not present

affected eggs/embryos/larvae

egg production: number <50% of expected


affected eggs/embryos/larvae

larva number hatched none (< 10 %)


egg production: number <50% of expected


larva number few (< 50 %) hatched


affected eggs/embryos/larvae

egg production: number


head appendages position irregular

antenna mostly not present

head appendages shape irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


abdomen segment sometimes number decreased

head & thorax segment random partially not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected


antenna shape irregular

legs size decreased

legs segment shape irregular

legs segment partially not present

antenna mostly not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


head & thorax & abdomen segment random not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


affected eggs/embryos/larvae


abdomen segment mostly number decreased

head & thorax & abdomen segment partially random not present

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

larva number few (< 50 %) hatched


abdomen mostly everted (inside-out)

larva mostly everted (inside-out)

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva number hatched none (< 10 %)


affected eggs/embryos/larvae

larva number few (< 50 %) hatched

egg production: number <50% of expected


affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected


larva number hatched none (< 10 %)

egg production: number <50% of expected


legs partially size decreased

legs partially irregular

abdomen segment number decreased

legs partially not present

affected eggs/embryos/larvae


Perhaps empty egg shells from hatched larvae.

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


larva dorsal partially irregular

larva dorsal partially not closed

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


egg production: number <50% of expected


larva potentially everted (inside-out)

Predominantly head and thorax are affected.

cuticle remnants with unidentifyable segments

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae


vertex triplet vertex triplet posterior not present

labrum potentially sometimes not present

labrum shape irregular

labrum position irregular

labrum partially split

antenna mostly not present

affected eggs/embryos/larvae


larva number few (< 50 %) hatched

egg production: number <50% of expected


larva segment potentially random number decreased

head & thorax appendages shape swollen

Specifically the head appendages are affected.

larva appendages shape irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


egg production: number <50% of expected

larva number hatched none (< 10 %)


cuticle remnants with some identifiable segments

abdomen segment number decreased

head appendages partially random not present

affected eggs/embryos/larvae


larva number few (< 50 %) hatched


head capsule potentially median irregular

abdomen partially everted (inside-out)

vertex triplet partially position irregular

larva partially dorsal irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva number hatched many (ca. 70-100 %)


larva number few (< 50 %) hatched

larva number hatched none (< 10 %)


larva number hatched none (< 10 %)


pygopodium not present

urogomphi not present

abdomen segment number decreased

pygopodium size decreased

urogomphi size decreased

posterior terminus irregular

affected eggs/embryos/larvae


affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected


larva number hatched none (< 10 %)

egg production: number <50% of expected


legs partially shape irregular

head appendages shape irregular

larva segment partially random not present

affected eggs/embryos/larvae


head & thorax constricted

affected eggs/embryos/larvae


affected eggs/embryos/larvae


egg production: number <50% of expected


egg production: number <50% of expected

larva number few (< 50 %) hatched


abdomen partially median circumference broader

abdomen circumference irregular

abdomen potentially ventral not closed

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number no/almost no eggs


antenna partially not present

head appendages position irregular

head capsule not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


head & thorax & abdomen segment random partially not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


head segment position irregular

abdomen segment number decreased

head segment partially mostly decreased

affected eggs/embryos/larvae

larva number hatched none (< 10 %)