embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

eclosion not fulfilled (died as pupa)


head & thorax segment random not present

abdomen segment number decreased

larva appendages partially position irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

eclosion not fulfilled (with pupal and adult features)


larva number hatched many (ca. 70-100 %)

adult lethality


eclosion not fulfilled (died as pupa)


leg musculature partially degenerated

leg musculature potentially partially broader

Specifically the muscles of the praetarsus are affected.

affected eggs/embryos/larvae

adult lethality


adult lethality

adult lethality


adult lethality

adult lethality

adult lethality

egg production: number no/almost no eggs


abdominal odoriferous glands darker

adult lethality


pygopodium orientation irregular

pygopods spread

urogomphi size decreased

posterior terminus shape irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

head & thorax & abdomen segment random not present

head posterior not fused

head & thorax & abdomen segment shape irregular

affected eggs/embryos/larvae

larval musculature random not present

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


legs femur potentially fused

legs tibiotarsus potentially fused

legs shortened

larva partially everted (inside-out)

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)

adult lethality


adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult nutritional state potentially bad (cachectic)

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality


antenna antennal flagellum not present

affected eggs/embryos/larvae

adult lethality

larval musculature random not present

larval musculature random rounded

larval musculature thinner

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)

adult lethality


adult lethality

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


abdomen segment number decreased

legs sometimes shortened

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)

adult lethality


head mostly not present

abdomen segment partially fused

abdomen segment number decreased

thorax segment number decreased

head appendages random not present

larva segment number decreased

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva potentially everted (inside-out)

abdomen segment number decreased

thorax segment number decreased

larva segment number decreased

affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality immediately after eclosion

eclosion not fulfilled (died as pupa)


adult lethality

egg production: number <50% of expected

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)


head & thorax & abdomen segment number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


larval musculature lateral rounded

larval musculature lateral sometimes shortened

larval musculature mostly not present

larval musculature lateral number decreased

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


cuticle remnants strongly fragmented (cuticle crumbs)

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

eclosion not fulfilled (with pupal and adult features)


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

larva number hatched many (ca. 70-100 %)

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


head appendages random not present

head capsule size decreased

affected eggs/embryos/larvae

adult lethality

adult lethality


affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

egg production: number no/almost no eggs

adult lethality


adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

larval musculature fluorescence decreased

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality


antenna median swollen

labial palp swollen

legs pretarsus swollen

maxillary palp swollen

affected eggs/embryos/larvae

larval musculature sometimes rounded

larval musculature anterior partially not present

larval musculature anterior mostly not present

leg musculature not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


eclosion not fulfilled (died as pupa)


adult lethality

adult lethality

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality


larva everted (inside-out)

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult nutritional state bad (cachectic)

adult lethality

adult lethality


affected eggs/embryos/larvae

adult lethality

adult lethality

adult lethality


adult lethality

head appendages position irregular

head & thorax & abdomen segment random not present

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


head appendages sometimes random not present

abdomen posterior rounded

thorax & abdomen segment number decreased

abdomen segment number decreased

thorax segment number decreased

head appendages position irregular

larva partially everted (inside-out)

sometimes remaining head separat from everted thorax/abdomen

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult lethality

adult lethality

adult lethality


legs segment shortened

head segment random number decreased

head & thorax segment irregular

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

adult lethality

adult lethality

larva number hatched none (< 10 %)

adult lethality


egg production: number <50% of expected


adult lethality

adult lethality

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected


abdominal odoriferous glands content darker

abdominal odoriferous glands content decreased

abdominal odoriferous glands size decreased

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

larva number few (< 50 %) hatched


adult lethality

adult lethality

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


legs everted (inside-out)

legs potentially fused

labrum shape irregular

antenna not present

egg shell partially surface with protrusion

abdomen segment not present

thorax segment number decreased

head capsule shape irregular

head & thorax & abdomen segment irregular

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

tropharium shortened

vitellarium complete not present

egg chamber complete not present

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality

adult lethality


adult lethality

vitellogenic egg chamber posterior fused

egg chamber sometimes stage sequence irregular

mature egg not present

adult lethality


adult lethality


larva number few (< 50 %) hatched

adult lethality


adult lethality

follicular epithelium partially potentially decreased

egg chamber number decreased

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched

adult lethality


adult lethality

egg production: number <50% of expected


affected eggs/embryos/larvae

adult lethality

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

egg shell potentially robustness decreased

larva number hatched none (< 10 %)

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)


head & thorax mostly not present

head & thorax segment number decreased

head mostly not present

head & thorax & abdomen segment number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


legs segment shortened

legs segment shape irregular

metathorax potentially transformed

mesothorax number increased

metathorax not present

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

larval musculature fluorescence decreased

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality


head appendages sometimes position irregular

legs potentially number decreased

labrum sometimes not present

head appendages sometimes not present

head & thorax not present

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


maxilla distal bent

maxilla shape irregular

labrum orientation irregular

antenna shortened

legs tibiotarsus shape irregular

larva partially posterior everted (inside-out)

legs femur shape irregular

antenna orientation irregular

larva complete everted (inside-out)

affected eggs/embryos/larvae

leg musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

eclosion not fulfilled (with pupal and adult features)


maxilla distal bent

maxilla shape irregular

labrum orientation irregular

antenna shortened

legs tibiotarsus shape irregular

legs femur shape irregular

antenna orientation irregular

larva complete everted (inside-out)

affected eggs/embryos/larvae

leg musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


larva number hatched many (ca. 70-100 %)

adult lethality


vitellogenic egg chamber partially number increased

mature egg not present

egg production: number <50% of expected

adult lethality


head complete not present

head capsule size decreased

mandible not present

labrum not present

legs sometimes shape irregular

mesothorax not present

posterior terminus complete present

affected eggs/embryos/larvae

abdomen segment number decreased

prothorax not present

antenna not present

head & thorax segment number decreased

embryo/egg without visible larval cuticle in cuticle preparation

larva number hatched none (< 10 %)

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality


adult lethality

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

head capsule mostly not present

abdominal segment 1 constricted

Constriction often affecting A1 or T3/-A2

head appendages random not present

abdomen partially constricted

head appendages position irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


egg production: number <50% of expected

adult lethality


adult lethality

adult lethality

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


maxilla shape irregular

head appendages mostly not present

mesothorax partially not present

thorax mostly not present

antenna not present

just on one side

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality


adult nutritional state bad (cachectic)


adult lethality

larva number few (< 50 %) hatched

adult lethality


adult lethality


head capsule decreased

labrum not present

head appendages potentially partially not present

legs proximal mostly not present

abdomen segment number decreased

head appendages shape irregular

larva segment number decreased

affected eggs/embryos/larvae

adult lethality


larva segment partially not present

abdomen constricted

larva appendages shortened

affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


legs shape irregular

labium present

head appendages mostly not present

abdomen segment number decreased

legs shortened

head size decreased

head & thorax & abdomen irregular

just one larva on slide

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


legs shape irregular

labium not present

prothorax not present

mandible not present

head & thorax irregular

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae


affected eggs/embryos/larvae

larva number hatched none (< 10 %)

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae


tropharium shortened

also empty. potentially due to preperation

vitellarium not present

egg chamber not present

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


head capsule not present

larva cuticle thinner

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality

eclosion not fulfilled (died as pupa)


egg production: number <50% of expected

ovary without detectable defects

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality


affected eggs/embryos/larvae

adult lethality

adult lethality

larva number few (< 50 %) hatched


adult lethality

adult lethality immediately after eclosion

eclosion not fulfilled (died as pupa)


affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


larva appendages shape irregular

affected eggs/embryos/larvae

adult lethality


larval musculature thinner

affected eggs/embryos/larvae

egg production: number <50% of expected

ovary without detectable defects

adult nutritional state potentially bad (cachectic)

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


adult lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

adult lethality

adult nutritional state bad (cachectic)

egg production: number no/almost no eggs


adult lethality

adult lethality


antenna not present

antenna shape irregular

labrum size decreased

abdomen partially constricted

head size decreased

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


abdomen segment number decreased

head appendages random number decreased

thorax & abdomen segment number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality

eclosion not fulfilled (died as pupa)


adult lethality

eclosion not fulfilled (died as pupa)


adult lethality


adult lethality

larval musculature partially rounded

larval musculature partially not present

larval musculature thinner

larval musculature mostly not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


urogomphi size decreased

affected eggs/embryos/larvae

adult lethality

abdomen musculature anterior partially not present

larva bent

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


adult lethality

adult lethality

adult lethality


affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

abdomen tracheal opening size irregular

head capsule size decreased

urogomphi potentially everted (inside-out)

prothorax potentially not present

abdomen segment number decreased

thorax segment number decreased

head appendages unilateral mostly not present

larva segment number decreased

affected eggs/embryos/larvae

tropharium structure irregular

previtellogenic egg chamber not present

vitellogenic egg chamber number decreased

mature egg not present

larva number few (< 50 %) hatched

adult lethality

egg production: number <50% of expected

eclosion not fulfilled (with pupal and adult features)

larva number few (< 50 %) hatched

kein Öl

egg production: number <50% of expected


larva number hatched many (ca. 70-100 %)

adult lethality

eclosion not fulfilled (died as pupa)


affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


head & thorax & abdomen segment number decreased

cuticle remnants with unidentifyable segments

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

ovary without detectable defects

adult lethality

adult nutritional state bad (cachectic)

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (died as pupa)


affected eggs/embryos/larvae

larva number few (< 50 %) hatched

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality


follicular epithelium potentially not present

egg chamber shape irregular

egg production: number no/almost no eggs

egg chamber number decreased

adult lethality


adult lethality

adult lethality


abdomen segment number decreased

thorax mostly not present

head shape irregular

larva segment number decreased

head partially remaining

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

eclosion not fulfilled (died as pupa)

affected eggs/embryos/larvae

adult lethality

ovary without detectable defects

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality

mature egg deposition 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

ovary without detectable defects


legs number increased

larva random constricted

affected eggs/embryos/larvae

adult lethality

adult lethality

adult lethality

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae


adult lethality


larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality

adult lethality


larva number few (< 50 %) hatched

egg production: number <50% of expected


adult lethality

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

adult lethality


previtellogenic egg chamber mostly not present

previtellogenic egg chamber shape irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva random segmentation/division not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


antenna pedicellus sometimes not present

labrum size decreased

labrum shape irregular

abdomen shape irregular

labrum not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult nutritional state bad (cachectic)

adult lethality

adult lethality


abdomen partially everted (inside-out)

abdomen potentially everted (inside-out)

abdomen circumference irregular

abdomen dv-orientation irregular

abdomen segment mostly not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


abdomen partially everted (inside-out)

abdomen circumference irregular

abdomen dv-orientation irregular

abdomen segment mostly not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


head appendages shape irregular

antenna not present

prothorax partially not present

head capsule mostly not present

head & thorax pattern irregular

affected eggs/embryos/larvae

adult lethality

adult lethality

eclosion not fulfilled (died as pupa)


vitellogenic egg chamber sometimes structure degenerated

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (with pupal and adult features)

eclosion not fulfilled (died as pupa)


adult nutritional state bad (cachectic)

adult lethality


affected eggs/embryos/larvae

adult lethality

adult lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality immediately after eclosion


abdomen posterior shape irregular

legs size decreased

affected eggs/embryos/larvae

larval musculature lateral potentially thinner

larval musculature fluorescence decreased

affected eggs/embryos/larvae

larva number hatched many (ca. 70-100 %)

adult lethality


legs cuticle shape irregular

posterior terminus shape irregular

abdomen bristle random number increased

thorax bristle random number increased

gena triplet bristle duplicated

larva bristle number increased

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature thinner

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


larva potentially partially everted (inside-out)

cuticle remnants strongly fragmented (cuticle crumbs)

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae


antenna potentially transformed

legs number increased

at antenna locus

antenna not present

affected eggs/embryos/larvae

larval musculature pattern irregular

spineless

affected eggs/embryos/larvae

adult lethality


adult lethality

egg production: number no/almost no eggs

adult lethality

adult lethality


larval musculature sometimes rounded

larval musculature thinner

abdomen musculature partially random not present

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

adult lethality


adult lethality

previtellogenic egg chamber late deposition irregular

previtellogenic egg chamber number decreased

yolk late deposition irregular

mature egg not present

larva number hatched none (< 10 %)


larva cuticle duplicated

larvae without detectable defects but die during shedding of the skin

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched

adult lethality

adult lethality


larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

adult lethality


head capsule shape irregular

head appendages structure irregular

head appendages shape irregular

thorax segment number decreased

legs podomer fused

posterior terminus not present

abdomen segment number decreased

larva appendages shape irregular

abdomen segmentation/division irregular

legs size decreased

head capsule size decreased

larva segment number decreased

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

egg production: number <50% of expected

adult lethality

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality


head appendages random shape irregular

posterior terminus not present

legs tibiotarsus sometimes fused

legs femur sometimes fused

abdomen segment number decreased

abdomen cuticle segmentation/division irregular

legs swollen

head capsule structure irregular

larva segmentation/division irregular

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality


maxillary palp orientation irregular

labium distal bent

affected eggs/embryos/larvae

adult lethality

adult lethality


larva segmentation/division irregular

thorax partially not present

abdomen segment number decreased

thorax potentially not present

head capsule shape irregular

larva segment random number decreased

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality

adult lethality


maxilla shape irregular

head appendages orientation irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 4 5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


abdomen bristle mostly not present

larva bristle mostly not present

head & thorax cuticle dorsal potentially robustness decreased

abdomen potentially partially everted (inside-out)

abdomen segment number decreased

thorax bristle mostly not present

head bristle number decreased

head & thorax bent

affected eggs/embryos/larvae

mature egg not present

vitellogenic egg chamber potentially late degenerated

yolk late deposition irregular

adult nutritional state potentially bad (cachectic)

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (with pupal and adult features)


embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

adult lethality

larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality


adult lethality

adult lethality

larva number hatched none (< 10 %)

adult lethality


adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

previtellogenic egg chamber orientation irregular

previtellogenic egg chamber position irregular

previtellogenic egg chamber potentially number decreased

tropharium structure irregular

mature egg not present

egg production: number no/almost no eggs


antenna antennal flagellum not present

labrum shape irregular

labrum size decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched

adult lethality

vertex triplet vertex triplet posterior unilateral not present

head potentially slightly shape irregular

vertex triplet vertex triplet posterior not present


adult lethality

adult lethality

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


head cuticle ventral structure irregular

head capsule potentially size decreased

maxilla cuticle proximal irregular

maxilla potentially orientation irregular

labrum not present

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality

labrum not present

vertex vertex triplet anterior not present

gena bristle number increased

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


legs podomer structure irregular

head appendages random not present

cuticle remnants with unidentifyable segments

head capsule structure irregular

thorax partially not present

head capsule size decreased

head & thorax segment mostly not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

eclosion not fulfilled (with pupal and adult features)


adult lethality

adult lethality


legs position irregular

head appendages random not present

head capsule mostly not present

legs number decreased

legs structure irregular

legs potentially size decreased

thorax & abdomen partially not present

thorax not present

abdomen segment number decreased

thorax partially not present

head capsule structure irregular

larva segment posterior number decreased

head

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


ovary without detectable defects

adult nutritional state bad (cachectic)

larva number hatched many (ca. 70-100 %)

egg production: number <50% of expected

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


posterior terminus not present

abdomen segment number decreased

affected eggs/embryos/larvae

adult lethality

adult lethality

abdomen sometimes number decreased

affected eggs/embryos/larvae


affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)

adult lethality


adult lethality


previtellogenic egg chamber potentially present

egg chamber number decreased

vitellogenic egg chamber early potentially degenerated

tropharium structure irregular

vitellogenic egg chamber late posteriorized

mature egg not present

adult lethality

egg production: number no/almost no eggs

adult lethality


abdomen bristle random number increased

thorax bristle random number increased

head bristle random number increased

larva bristle random number increased

affected eggs/embryos/larvae

adult lethality

adult lethality immediately after eclosion


tropharium structure irregular

vitellarium size decreased

egg chamber number decreased

mature egg not present

adult lethality

larva number hatched none (< 10 %)


adult lethality

adult lethality

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

mature egg potentially deposition irregular

previtellogenic egg chamber number decreased

vitellarium anterior constricted

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


larva cuticle duplicated

larvae are not able to shed off old skin

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult lethality

adult lethality

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality


antenna distal structure irregular

head appendages random shape irregular

posterior terminus not present

abdomen segment number decreased

head appendages orientation irregular

affected eggs/embryos/larvae

abdominal odoriferous glands colour darker

adult lethality

larval musculature fluorescence decreased

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult lethality


adult lethality

adult lethality

head appendages random not present

abdomen ventral irregular

head capsule potentially not present

abdomen dorsal irregular

head appendages position irregular

head & thorax & abdomen segment random not present

affected eggs/embryos/larvae

adult lethality


adult lethality

egg production: number <50% of expected

adult lethality


head bristle not present

head appendages shape irregular

thorax bristle not present

mesothoracic tracheal opening not present

abdomen bristle not present

abdomen tracheal opening not present

abdomen shape irregular

larva shape irregular

legs swollen

larva shortened

larva appendages swollen

posterior terminus not present

legs podomer fused

head appendages swollen

larva bristle not present

cuticle remnants strongly fragmented (cuticle crumbs)

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

mature egg deposition 5 >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

adult lethality


previtellogenic egg chamber potentially number decreased

previtellogenic egg chamber orientation irregular

vitellogenic egg chamber late structure irregular

tropharium surface irregular

mature egg not present

egg production: number <50% of expected

adult lethality


thorax partially anterior constricted

abdomen ventral potentially not closed

thorax anterior ventral bent

affected eggs/embryos/larvae

prothorax cuticle potentially posterior decreased

larval musculature random rounded

probably dying

larval musculature fluorescence broader

larvae die after hatching

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality

larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality


head appendages orientation irregular

labrum position irregular

antenna not present

affected eggs/embryos/larvae

adult lethality

adult lethality


legs potentially number increased

labium structure irregular

affected eggs/embryos/larvae

labium potentially transformed

into 1 leg

prothorax musculature dorsal pattern irregular

embryos in egg not quite scorable

affected eggs/embryos/larvae

adult lethality


abdomen cuticle structure irregular

labrum not present

antenna not present

abdomen shape irregular

head capsule mostly not present

cuticle remnants with unidentifyable segments

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


abdominal odoriferous glands content fragmented

abdominal odoriferous glands colour darker

prothoracic odoriferous glands colour darker

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

adult lethality


adult lethality

affected eggs/embryos/larvae


maxilla swollen

antenna potentially size decreased

affected eggs/embryos/larvae

antenna proximal shape irregular

abdomen musculature anterior partially rounded

abdomen musculature anterior lateral not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality


larval musculature fluorescence decreased

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

adult lethality

eclosion not fulfilled (with pupal and adult features)


abdomen cuticle structure irregular

head capsule structure irregular

abdomen not present

labium structure irregular

head & thorax potentially fused

abdomen segment number decreased

thorax partially not present

labium not present

head & thorax partially not present

affected eggs/embryos/larvae

larval musculature thinner

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


abdomen segmentation/division irregular

legs podomer sometimes fused

head appendages orientation irregular

legs potentially fused

legs structure irregular

thorax potentially partially not present

abdomen segment number decreased

legs number decreased

head capsule structure irregular

larva segmentation/division irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult lethality


adult lethality

egg production: number no/almost no eggs

adult lethality

adult lethality

adult lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


adult lethality

adult nutritional state bad (cachectic)


affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

adult lethality

adult lethality


adult lethality

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

adult lethality


affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched


prothoracic odoriferous glands darker

adult lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)


prothoracic odoriferous glands darker

adult lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

larva number hatched none (< 10 %)


larva segment anterior number decreased

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

ovary without detectable defects

larva number hatched none (< 10 %)

adult lethality


larva number hatched many (ca. 70-100 %)

larva number few (< 50 %) hatched


adult lethality


vitellogenic egg chamber potentially shape irregular

previtellogenic egg chamber potentially not present

adult lethality

adult nutritional state potentially bad (cachectic)

adult lethality

adult nutritional state bad (cachectic)


adult lethality

egg production: number <50% of expected

adult lethality

ovary without detectable defects


adult lethality

adult lethality

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number few (< 50 %) hatched


adult lethality

adult lethality

adult lethality

adult lethality

adult nutritional state


larval musculature lateral size shortened

affected eggs/embryos/larvae


gena triplet gena triplet dorsal not present

affected eggs/embryos/larvae

abdominal odoriferous glands size increased

abdominal odoriferous glands content opaque

adult lethality

adult lethality


adult lethality

adult lethality


adult lethality

adult lethality

eclosion not fulfilled (died as pupa)


adult lethality

adult lethality

egg production: number <50% of expected

adult lethality

adult lethality


egg production: number <50% of expected

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

adult lethality

adult lethality

larva number hatched none (< 10 %)


gena triplet bristle number increased

antenna scapus structure not present

Stigma missing

antenna antennal flagellum number increased

affected eggs/embryos/larvae

egg production: number <50% of expected

adult lethality

adult lethality immediately after eclosion

eclosion not fulfilled (with pupal and adult features)

mature egg deposition 2 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:


adult lethality

labium potentially structure broader

labium shape irregular

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

adult lethality


previtellogenic egg chamber mostly fused

vitellogenic egg chamber mostly not present

egg chamber structure irregular

egg production: number no/almost no eggs


adult lethality

larva number hatched many (ca. 70-100 %)


head appendages shape irregular

legs sometimes shape irregular

head size decreased

affected eggs/embryos/larvae

adult lethality

adult lethality


labium potentially structure broader

labium shape irregular

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality


adult lethality

adult lethality

adult lethality


adult lethality

adult lethality

adult lethality

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva shape irregular

legs potentially transformed

potentially transformed into maxilla and labium

legs not present

labium number increased

larva size decreased

abdomen segment number decreased

thorax segment number decreased

maxilla number increased

larva segment potentially number decreased

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

adult lethality

egg colour darker

larva number hatched none (< 10 %)


head bristle pattern irregular

head bristle number increased

head capsule shape irregular

urogomphi size decreased

posterior terminus shape irregular

abdomen circumference decreased

abdomen partially everted (inside-out)

head capsule size decreased

affected eggs/embryos/larvae

adult lethality

egg production: number <50% of expected

adult lethality

larva number few (< 50 %) hatched


adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

previtellogenic egg chamber mostly potentially not present

vitellogenic egg chamber shape irregular

larva number few (< 50 %) hatched


head & thorax partially not present

larva number hatched none (< 10 %)

abdomen segment potentially number decreased

thorax not present

head not present

head & thorax not present

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

adult lethality

adult lethality

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

adult lethality

adult lethality

adult lethality

adult lethality


affected eggs/embryos/larvae

adult lethality


affected eggs/embryos/larvae

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:


affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

eclosion not fulfilled (with pupal and adult features)


thorax partially unilateral fused

legs partially not present

thorax symmetry irregular

labium sometimes not present

head & thorax segment unilateral fused

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

ovary

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality


cuticle remnants without visible segmentation but ap-polarity

larva number hatched none (< 10 %)

cuticle remnants strongly fragmented (cuticle crumbs)

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae


adult lethality

larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

adult lethality


adult lethality

adult lethality immediately after eclosion


adult lethality

egg production: number <50% of expected

adult lethality


egg production: number no/almost no eggs

adult lethality


ovary without detectable defects

mature egg deposition 4 5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality

adult lethality


adult lethality


affected eggs/embryos/larvae

adult lethality

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

adult lethality

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

head mostly remaining

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


metathorax not present

mesothorax not present

head appendages random not present

larva partially everted (inside-out)

abdomen everted (inside-out)

thorax partially not present

head appendages shape irregular

larva fragmented

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


larva number few (< 50 %) hatched


abdomen potentially partially everted (inside-out)

posterior terminus not present

head size decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

ovary without detectable defects

adult nutritional state potentially bad (cachectic)

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

adult lethality

adult lethality


larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


egg production: number <50% of expected

adult lethality


ovary without detectable defects

adult lethality

egg production: number <50% of expected

adult lethality


larva segment number decreased

larva cuticle dorsal not fused

abdomen segment number decreased

thorax segment number decreased

head appendages not present

larva everted (inside-out)

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


adult nutritional state bad (cachectic)

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality

adult lethality


larva everted (inside-out)

abdomen segment number decreased

thorax segment number decreased

head capsule size decreased

thorax & abdomen segment number decreased

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality


abdomen segment number decreased

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality

embryo/egg not developed/not fertilized

2 eggs

affected eggs/embryos/larvae

yolk late structure fragmented


larva number few (< 50 %) hatched

adult lethality


cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


mature egg not present

adult lethality

egg chamber not present

vitellarium size decreased


vertex triplet anterior not present

head appendages orientation irregular

antenna shape irregular

labrum size decreased

labrum not present

head shape irregular

head size decreased

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


abdomen segment number decreased

legs size decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


larva partially everted (inside-out)

larva everted (inside-out)

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


adult lethality

adult nutritional state bad (cachectic)

adult lethality


head appendages random not present

head capsule not present

head capsule size decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult lethality

adult lethality

adult lethality


adult lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


egg production: number <50% of expected

adult lethality

adult lethality


abdomen segment number decreased

thorax segment number decreased

head capsule size decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


antenna partially shape thinner

antenna partially not present

head appendages orientation irregular

head capsule size decreased

vertex triplet seta partially not present

affected eggs/embryos/larvae

larval musculature fluorescence decreased

Decreased fluorescence included

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality

adult lethality


adult lethality

adult nutritional state bad (cachectic)

adult lethality

adult lethality


labrum size decreased

mandible potentially transformed

mandible not present

maxilla number increased

affected eggs/embryos/larvae

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality


labrum size decreased

mandible potentially transformed

mandible not present

maxilla number increased

affected eggs/embryos/larvae

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality

adult lethality


labrum size decreased

mandible potentially transformed

mandible not present

maxilla number increased

affected eggs/embryos/larvae

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


labrum size decreased

mandible potentially transformed

mandible not present

maxilla number increased

affected eggs/embryos/larvae

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality

adult lethality

adult lethality


labrum size decreased

mandible potentially transformed

mandible not present

maxilla number increased

affected eggs/embryos/larvae

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


labrum size decreased

mandible potentially transformed

mandible not present

maxilla number increased

affected eggs/embryos/larvae

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality

larva number hatched none (< 10 %)


labrum size decreased

mandible potentially transformed

mandible not present

maxilla number increased

affected eggs/embryos/larvae

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality

larva sometimes everted (inside-out)

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

abdominal odoriferous glands less secretion


labrum size decreased

mandible potentially transformed

mandible not present

maxilla number increased

affected eggs/embryos/larvae

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality

odoriferous glands

abdominal odoriferous glands colour not present

abdominal odoriferous glands content darker

abdominal odoriferous glands colour darker

prothoracic odoriferous glands colour darker


labrum size decreased

mandible potentially transformed

mandible not present

maxilla number increased

affected eggs/embryos/larvae

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality

adult lethality

larval musculature thinner

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


labrum size decreased

mandible potentially transformed

mandible not present

maxilla number increased

affected eggs/embryos/larvae

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality

affected eggs/embryos/larvae


follicular epithelium potentially posterior amount decreased

egg chamber number decreased

vitellogenic egg chamber shape irregular

mature egg not present

adult lethality


antenna antennal flagellum not present

pygopodium not present

urogomphi not present

labial palp potentially fused

abdomen segment number decreased

legs segment distal mostly not present

maxilla size decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


antenna antennal flagellum not present

pygopodium not present

urogomphi not present

labial palp potentially fused

abdomen segment number decreased

legs segment distal mostly not present

maxilla size decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

legs femur partially not present

abdomen mostly number decreased

legs tibiotarsus mostly not present

antenna pedicellus mostly not present

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


eclosion not fulfilled (with pupal and adult features)


adult lethality

egg chamber structure irregular

egg chamber mostly not present

tropharium structure degenerated

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


adult lethality

adult lethality


gena triplet bristle number increased

head bristle pattern irregular

head capsule potentially size decreased

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


antenna antennal flagellum not present

head capsule size decreased

labrum not present

labium size decreased

antenna orientation irregular

affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

larval musculature pattern irregular

Decreased fluorescence included

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


legs segment mostly not present

head capsule size decreased

abdomen not present

thorax segment number decreased

head appendages shape irregular

thorax & abdomen segment number decreased

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

larva number few (< 50 %) hatched


legs segment mostly not present

head capsule size decreased

abdomen not present

thorax segment number decreased

head appendages shape irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality


cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

mature egg not present

larva number hatched none (< 10 %)

adult lethality


abdomen circumference decreased

larva partially everted (inside-out)

affected eggs/embryos/larvae

ovary without detectable defects

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality

adult lethality

adult lethality

larval musculature fluorescence not present

affected eggs/embryos/larvae

larva number hatched many (ca. 70-100 %)


affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult nutritional state bad (cachectic)

adult lethality

egg production: number no/almost no eggs

adult lethality

adult lethality


adult lethality

adult nutritional state bad (cachectic)

adult lethality


adult lethality

adult lethality

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


head appendages potentially slightly size decreased

head capsule size decreased

affected eggs/embryos/larvae


head capsule potentially not present

head appendages random not present

larva complete everted (inside-out)

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


larva partially everted (inside-out)

larva everted (inside-out)

larval musculature random not present

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality


head capsule partially not present

procephalic head partially not present

procephalic appendages random mostly shape irregular

Mostly Antenna is absent and Labrum'shape is irregular as result head capsule is shortened.

affected eggs/embryos/larvae

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae


egg production: number <50% of expected


adult lethality


legs segment partially fused

larva dorsal pattern irregular

antenna antennal flagellum mostly not present

head appendages size shortened

legs shape irregular

abdomen segment partially shape irregular

legs size shortened

Leg shape is irregular & can not differenciate between Femur & Tt.

head appendages structure irregular

head & thorax appendages irregular

affected eggs/embryos/larvae

larval musculature random not present

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

larva number few (< 50 %) hatched


abdomen ventral circumference irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


abdomen segment sometimes number decreased

legs shape irregular

head appendages random not present

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

affected eggs/embryos/larvae


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

ovary without detectable defects

adult lethality

adult nutritional state potentially bad (cachectic)


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


embryo/egg with embryonic tissue, no cuticle, no fluorescence

adult lethality

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

affected eggs/embryos/larvae

adult lethality

larva dorsal not closed

larva everted (inside-out)

abdomen circumference decreased

larva partially everted (inside-out)

affected eggs/embryos/larvae

larval musculature pattern irregular


head & thorax & abdomen segment random not present

head posterior not fused

head & thorax & abdomen segment shape irregular

affected eggs/embryos/larvae

larval musculature random not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


head posterior not fused

affected eggs/embryos/larvae

larval musculature random not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

larva number few (< 50 %) hatched


larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)


head & thorax appendages shape irregular

affected eggs/embryos/larvae


head & thorax & abdomen segment irregular

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


mature egg not present

vitellogenic egg chamber late shape irregular

egg production: number no/almost no eggs


embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality


head appendages potentially random not present

head capsule shape irregular

affected eggs/embryos/larvae

adult lethality


affected eggs/embryos/larvae

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)


head & thorax & abdomen segment random irregular

affected eggs/embryos/larvae

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


eclosion not fulfilled (with pupal and adult features)


adult lethality

adult lethality


adult nutritional state potentially bad (cachectic)

adult lethality


mature egg stage sequence irregular

vitellogenic egg chamber shape irregular

previtellogenic egg chamber not present

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality


head & thorax & abdomen segment random not present

abdomen segment number decreased

head & thorax & abdomen irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

egg chamber mostly not present

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected


cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

larva number hatched many (ca. 70-100 %)


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


head appendages random position irregular

affected eggs/embryos/larvae

adult lethality


head & thorax appendages potentially irregular

affected eggs/embryos/larvae

larval musculature random irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


mature egg not present

head & thorax & abdomen segment irregular

affected eggs/embryos/larvae

vitellogenic egg chamber late potentially shape irregular

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

egg production: number <50% of expected


adult lethality

adult nutritional state bad (cachectic)


cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality


thorax & abdomen circumference decreased

head & thorax & abdomen shape irregular

larva partially potentially everted (inside-out)

Abdomen and thorax probably not deleted but reduced to a string of cuticle. Unclear whether these remains are inside out.

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


vitellogenic egg chamber fused

tropharium size decreased

previtellogenic egg chamber not present

adult nutritional state potentially bad (cachectic)

egg production: number <50% of expected

adult lethality


embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


legs segment potentially fused

Femur and Tibiatarsus are mostly affected.

head & thorax & abdomen segment random irregular

legs shape irregular

head & thorax & abdomen segment random not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

larva number hatched many (ca. 70-100 %)

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


abdomen segment number decreased

thorax dorsal partially not fused

urogomphi mostly present

thorax segment number decreased

larva segment number decreased

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

eclosion not fulfilled (died as pupa)


affected eggs/embryos/larvae

abdominal odoriferous glands content decreased

odoriferous glands darker

larval musculature pattern irregular

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


vitellogenic egg chamber late potentially degenerated

mature egg not present

egg production: number no/almost no eggs

adult lethality


affected eggs/embryos/larvae

ovary without detectable defects

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality

adult lethality


adult lethality

adult lethality

adult lethality


adult lethality

adult lethality

adult lethality

head & thorax & abdomen segment potentially shape irregular

affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult nutritional state bad (cachectic)

adult lethality


vitellogenic egg chamber late shape rounded

mature egg not present

previtellogenic egg chamber position irregular

previtellogenic egg chamber number increased

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

adult lethality

adult lethality

adult lethality


adult lethality

abdomen segment number decreased

thorax segment number decreased

thorax & abdomen segment number decreased

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

egg robustness decreased

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)


adult lethality


adult lethality

vitellarium size increased

vitellogenic egg chamber late number increased

mature egg not present

adult lethality


adult lethality

egg production: number <50% of expected

adult lethality


affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

ovary without detectable defects

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected

eclosion not fulfilled (died as pupa)

adult lethality


adult lethality

larva number hatched many (ca. 70-100 %)

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


egg chamber sometimes complete not present

yolk late potentially degenerated

mature egg not present

adult lethality


head appendages partially not present

larva segment number decreased

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


egg production: number <50% of expected

adult lethality


mature egg not present

yolk late amount decreased

adult lethality

adult lethality


cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

eclosion not fulfilled (died as pupa)


larva number few (< 50 %) hatched


adult lethality

adult lethality

adult lethality


adult lethality

adult lethality


adult lethality

head not present

legs potentially partially fused

thorax segment partially number decreased

abdomen segment number decreased

legs size decreased

head appendages position irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality immediately after eclosion


adult lethality

egg production: number no/almost no eggs

adult lethality

adult lethality


affected eggs/embryos/larvae

adult lethality

3 of 3 eggs

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

egg production: number <50% of expected

eclosion not fulfilled (died as pupa)

egg production: number <50% of expected


adult lethality


adult lethality

adult nutritional state bad (cachectic)

adult lethality

adult lethality

eclosion not fulfilled (died as pupa)


affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


head appendages random not present

abdomen segment number decreased

thorax segment number decreased

head capsule size decreased

larva segment number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality


thorax segment unilateral number decreased

thorax & abdomen cuticle dorsal partially not fused

affected eggs/embryos/larvae

adult lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

adult nutritional state potentially bad (cachectic)

adult nutritional state bad (cachectic)

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected


larval musculature random not present

larval musculature random rounded

larval musculature late fluorescence broader

meaning they died after hatching

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


abdomen segment number decreased

affected eggs/embryos/larvae

larval musculature fluorescence decreased

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


head partially shape irregular

A dent near the labrum

affected eggs/embryos/larvae

adult lethality

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


abdomen segment potentially transformed

legs size decreased

abdomen segment number decreased

thorax segment number increased

head appendages shape irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


ovary without detectable defects

mature egg not present

yolk late potentially degenerated

vitellogenic egg chamber late potentially degenerated

vitellogenic egg chamber late number increased

larva number hatched many (ca. 70-100 %)

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected


urogomphi size decreased

affected eggs/embryos/larvae

larval musculature lateral partially rounded

larval musculature dorsal partially rounded

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

embryo/egg not developed/not fertilized

2 out of 2

affected eggs/embryos/larvae

mature egg not present

previtellogenic egg chamber number decreased

tropharium structure irregular


adult lethality

ovary without detectable defects

adult lethality

egg production: number <50% of expected

egg production: number <50% of expected


adult lethality

adult lethality


adult lethality

adult lethality

adult lethality


gena triplet gena triplet posterior not present

bell row not present

affected eggs/embryos/larvae

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

adult lethality


abdomen segment number decreased

thorax segment number decreased

head appendages partially not present

larva segment number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

tropharium potentially degenerated

egg chamber number decreased

mature egg not present

egg production: number no/almost no eggs

adult lethality


larval musculature pattern irregular

affected eggs/embryos/larvae

ovary without detectable defects

egg production: number <50% of expected


larval musculature pattern irregular

affected eggs/embryos/larvae

ovary without detectable defects

egg production: number <50% of expected

adult lethality


adult lethality


adult nutritional state bad (cachectic)


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

egg production: number <50% of expected


previtellogenic egg chamber number increased

vitellogenic egg chamber potentially fused

previtellogenic egg chamber position irregular

vitellogenic egg chamber late rounded

vitellogenic egg chamber potentially late degenerated

follicular epithelium surface irregular

potentially detached from basal membrane

mature egg not present

eclosion not fulfilled (with pupal and adult features)


affected eggs/embryos/larvae

adult lethality


adult lethality

adult lethality

adult lethality immediately after eclosion


larval musculature partially random rounded

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality


abdominal odoriferous glands content decreased

prothoracic odoriferous glands partially darker

prothoracic odoriferous glands darker

abdominal odoriferous glands darker

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

2 out of 2

vitellogenic egg chamber not present

mature egg not present


abdomen partially circumference decreased

affected eggs/embryos/larvae

larva number hatched many (ca. 70-100 %)

adult lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

adult lethality

eclosion not fulfilled (died as pupa)


adult lethality

adult lethality


adult lethality

adult lethality

adult lethality

adult lethality


larva number hatched none (< 10 %)


thorax constricted

head appendages random not present

head & thorax appendages partially not present

abdomen number decreased

thorax segment not present

More often prothorax segment are absent.

head capsule not present

head & thorax segment partially not present

May be all blastoderm segments are affected (gnathal and thorasic).

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


ovary without detectable defects

adult nutritional state

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality


affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

previtellogenic egg chamber shape irregular

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

egg production: number no/almost no eggs


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


head & thorax & abdomen segment random not present

larva complete everted (inside-out)

affected eggs/embryos/larvae

abdomen musculature lateral posterior not present

embryo/egg not developed/not fertilized

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


prothoracic leg partially fused

Femur and Tibiatarus are mainly affected.  Specifically, Only one leg of prothorax is affected.

abdomen segment number decreased

legs size decreased

larva dorsal ventral pattern irregular

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

larval musculature fluorescence decreased

larval musculature thinner

affected eggs/embryos/larvae

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

ovary without detectable defects

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

adult lethality


embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

ovary without detectable defects

egg production: number <50% of expected


adult lethality

adult nutritional state bad (cachectic)

adult lethality


larva dorsal not closed

affected eggs/embryos/larvae

larval musculature thinner

larval musculature random not present

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


previtellogenic egg chamber symmetry irregular

Position & size of eggs of previtellogenic are altered.


previtellogenic egg chamber potentially not present

larva number hatched many (ca. 70-100 %)

egg production: number <50% of expected


adult lethality

adult lethality immediately after eclosion


adult lethality

larval musculature potentially fluorescence not present

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult nutritional state

egg production: number <50% of expected

egg production: number <50% of expected


adult lethality

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult nutritional state potentially bad (cachectic)

adult lethality

adult nutritional state bad (cachectic)

adult lethality


cuticle remnants with some identifiable segments

affected eggs/embryos/larvae


head capsule not present

head capsule dorsal size decreased

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


abdomen anterior ventral cuticularisation not closed

affected eggs/embryos/larvae

larval musculature potentially fused

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality


affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched


adult lethality

eclosion not fulfilled (died as pupa)


adult lethality

egg production: number no/almost no eggs

adult lethality


cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

adult lethality

larval musculature thinner

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

eclosion not fulfilled (with pupal and adult features)


adult nutritional state


ovary without detectable defects

adult nutritional state bad (cachectic)

adult lethality


adult nutritional state bad (cachectic)

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva segment pattern irregular

abdomen dorsal pattern irregular

larva segment random not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

abdomen musculature random not present

affected eggs/embryos/larvae


head capsule not present

thorax segment partially not present

head appendages random not present

head & thorax & abdomen random not present

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)

adult lethality


vitellogenic egg chamber deposition degenerated

previtellogenic egg chamber mostly not present

ovary without detectable defects

egg production: number <50% of expected


posterior terminus shape irregular

urogomphi not present

pygopodium not present

urogomphi shape irregular

affected eggs/embryos/larvae

abdomen segment number decreased

larval musculature random not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality


abdominal odoriferous glands mostly content decreased

larva number hatched many (ca. 70-100 %)

adult lethality


mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


cuticle remnants

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

previtellogenic egg chamber shape irregular

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


pygopodium not present

urogomphi not present

abdomen segment number # of remaining segments  decreased

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


head & thorax & abdomen segment random not present

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature random not present

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched

adult lethality


ovary without detectable defects

mature egg deposition 4 >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected


affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

previtellogenic egg chamber orientation irregular

previtellogenic egg chamber number increased

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

adult lethality


larva bristle number increased

thorax bristle random number increased

gena triplet gena triplet dorsal number increased

larva bristle random not present

affected eggs/embryos/larvae

larval musculature fluorescence decreased

larval musculature pattern irregular

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 4 5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (with pupal and adult features)


affected eggs/embryos/larvae

adult lethality

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, no fluorescence

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality


adult lethality

adult lethality

posterior terminus shape irregular

abdomen segment number decreased

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

adult lethality


mesothorax circumference decreased

antenna proximal size decreased

affected eggs/embryos/larvae

prothorax musculature partially lateral not present

larval musculature pattern irregular

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


adult lethality

larva number hatched many (ca. 70-100 %)

adult lethality


ovary without detectable defects

egg production: number <50% of expected

adult lethality


ovary without detectable defects

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


ovary without detectable defects

adult lethality

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality


adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae


larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality


bell row anteroposterior-axis elongated

vertex triplet vertex triplet ventral potentially position ventralized

gena triplet potentially position irregular

head bristle pattern irregular

head bristle number decreased

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae


abdomen segment potentially fused

abdomen bristle pattern irregular

posterior terminus shape irregular

abdomen segment number decreased

abdomen shape irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

larval musculature dorsal median shape irregular

affected eggs/embryos/larvae

adult lethality

egg production: number <50% of expected

adult lethality


larval musculature pattern irregular

affected eggs/embryos/larvae

ovary without detectable defects

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality

adult lethality


adult nutritional state bad (cachectic)

adult nutritional state potentially bad (cachectic)

larva number hatched none (< 10 %)

egg production: number no/almost no eggs


larva partially everted (inside-out)

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality


adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

adult lethality

adult lethality


larva partially everted (inside-out)

larva everted (inside-out)

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

previtellogenic egg chamber orientation irregular

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

adult lethality


affected eggs/embryos/larvae


ovary without detectable defects

egg production: number <50% of expected

adult lethality


adult lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


larva dorsal not closed

vertex triplet vertex triplet posterior not present

head appendages random not present

head capsule size decreased

larva partially everted (inside-out)

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality