legs size shortened

affected eggs/embryos/larvae

adult lethality

egg production: number <50% of expected

adult lethality


labrum shape irregular

vertex triplet vertex triplet posterior not present

vertex triplet vertex triplet anterior not present

labrum size decreased

affected eggs/embryos/larvae

larval musculature partially not present

affected eggs/embryos/larvae

larva number hatched many (ca. 70-100 %)

egg production: number <50% of expected

eclosion not fulfilled (with pupal and adult features)


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

mature egg not present

previtellogenic egg chamber orientation irregular

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality


adult lethality

adult lethality


adult lethality

adult lethality

legs sometimes number decreased

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae


adult lethality

adult lethality

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


abdomen segment number decreased

head shape irregular

mostly legs

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality

previtellogenic egg chamber number decreased

lateral oviduct degenerated

mature egg not fused

vitellarium size decreased

vitellogenic egg chamber degenerated

previtellogenic egg chamber not present

adult nutritional state bad (cachectic)


cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality


affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larval musculature fluorescence decreased

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

adult lethality


affected eggs/embryos/larvae

egg production: number <50% of expected

head appendages partially not present

head capsule partially size decreased

head & thorax sometimes partially not present

affected eggs/embryos/larvae


affected eggs/embryos/larvae

egg production: number <50% of expected


adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality


pygopodium shape irregular

urogomphi orientation irregular

urogomphi number decreased

urogomphi not present

posterior terminus shape irregular

affected eggs/embryos/larvae

larval musculature random not present

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

previtellogenic egg chamber shape irregular

mature egg not present

adult lethality


eclosion not fulfilled (died as pupa)


larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

eclosion not fulfilled (died as pupa)


larva dorsal bent

larva partially everted (inside-out)

larva everted (inside-out)

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


legs tibiotarsus size decreased

legs tibiotarsus shape irregular

affected eggs/embryos/larvae

adult lethality

adult lethality

adult lethality immediately after eclosion


urogomphi not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult nutritional state potentially bad (cachectic)

adult lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

adult lethality


larva number few (< 50 %) hatched

adult lethality

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

egg colour darker


larval musculature thinner

abdomen musculature specific subset not present

larval musculature random rounded

larval musculature lateral partially not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


larva number hatched many (ca. 70-100 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)

eclosion not fulfilled (died as pupa)


bell row potentially orientation irregular

vertex triplet potentially orientation irregular

gena triplet bristle potentially number increased

affected eggs/embryos/larvae

adult lethality

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number no/almost no eggs

adult lethality


adult lethality

adult lethality


adult lethality


head shape irregular

mandible shape rounded

mandible unilateral not present

abdomen segment number decreased

head appendages orientation irregular

cuticle remnants with some identifiable segments

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 5 >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality

adult lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


adult lethality

mature egg not present

tropharium structure irregular

previtellogenic egg chamber not present

egg chamber stage sequence irregular

previtellogenic egg chamber potentially number increased

previtellogenic egg chamber orientation irregular

adult lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


legs tibiotarsus potentially fused

legs trochanter not present

legs size decreased

gena triplet gena triplet anterior not present

gena triplet gena triplet posterior not present

gena triplet bristle number decreased

legs femur potentially fused

maxilla bristle number decreased

larva dorsal bent

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

egg colour darker

larva number hatched none (< 10 %)


adult lethality

egg production: number >150% of expected


larval musculature pattern irregular

affected eggs/embryos/larvae

abdominal odoriferous glands colour darker

mature egg deposition 4 5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched many (ca. 70-100 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


affected eggs/embryos/larvae

adult lethality


larva partially everted (inside-out)

thorax & abdomen dorsal not closed

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

egg production: number <50% of expected

adult lethality


egg production: number <50% of expected

adult lethality


affected eggs/embryos/larvae

adult lethality

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult nutritional state bad (cachectic)

adult nutritional state potentially bad (cachectic)

larva number hatched none (< 10 %)

adult lethality

adult lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

ovary without detectable defects

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


head appendages shape irregular

head capsule size decreased

head shape irregular

cuticle remnants strongly fragmented (cuticle crumbs)

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

adult lethality

head capsule mostly not present

labium size decreased

labrum mostly not present

antenna mostly not present

affected eggs/embryos/larvae


adult lethality

adult lethality

adult lethality

egg production: number no/almost no eggs

adult lethality

adult lethality


adult lethality

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

adult lethality

ovary without detectable defects


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality


prothorax musculature specific subset oblique not present

prothorax musculature specific subset oblique thinner

or missing

affected eggs/embryos/larvae

adult lethality


posterior terminus shape irregular

abdomen posterior shape irregular

affected eggs/embryos/larvae

adult lethality

larval musculature fluorescence broader

Larvae look wildtypish within their eggshells. As soon as they hatch they seem to be dieing

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (died as pupa)


adult lethality

adult lethality


adult lethality

adult lethality

abdominal odoriferous glands size decrease


adult lethality

adult lethality

eclosion not fulfilled (died as pupa)


mature egg not present

vitellogenic egg chamber late structure irregular

yolk structure irregular

vitellogenic egg chamber late shape irregular

larva number hatched none (< 10 %)

adult lethality


adult lethality

larva number hatched none (< 10 %)

adult lethality


larva dorsal not closed

larva everted (inside-out)

larva partially everted (inside-out)

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

egg production: number <50% of expected

adult lethality

adult lethality immediately after eclosion


larva dorsal not closed

larva everted (inside-out)

larva partially everted (inside-out)

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

egg production: number <50% of expected

adult lethality


larva dorsal not closed

larva everted (inside-out)

larva partially everted (inside-out)

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

egg production: number <50% of expected

adult lethality

adult lethality


antenna unilateral not present

labrum not present

affected eggs/embryos/larvae

ovary without detectable defects

egg production: number <50% of expected


affected eggs/embryos/larvae

adult lethality

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

affected eggs/embryos/larvae

adult lethality


affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


labrum not present

mandible not present

antenna not present

head mostly not present

larva dorsal not closed

affected eggs/embryos/larvae

adult lethality

abdomen musculature random not present

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality


adult lethality

adult lethality

labium shape irregular

labrum size decreased

affected eggs/embryos/larvae

adult lethality

adult lethality


larval musculature pattern irregular

larval musculature lateral thinner

larval musculature fluorescence decreased

affected eggs/embryos/larvae

previtellogenic egg chamber sometimes not present

tropharium potentially size decreased

adult lethality

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality


adult lethality

adult lethality


legs shape irregular

labium potentially not present

legs partially potentially fused

head shape irregular

larva dorsal not closed

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

egg production: number <50% of expected

adult lethality

mature egg not present

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)


adult lethality

previtellogenic egg chamber position irregular

tropharium structure irregular

vitellogenic egg chamber late number decreased

mature egg not present

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


previtellogenic egg chamber shape bent

adult nutritional state bad (cachectic)

adult lethality


legs tibiotarsus size shortened

head antennal flagellum not present

affected eggs/embryos/larvae

adult lethality

adult lethality


eclosion not fulfilled (died as pupa)


eclosion not fulfilled (died as pupa)

larva number few (< 50 %) hatched

egg production: number <50% of expected


eclosion not fulfilled (died as pupa)

adult lethality


eclosion not fulfilled (died as pupa)

larva complete everted (inside-out)

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larval musculature not present

abdomen seta pattern irregular

abdomen seta shape bent

legs size decreased

head capsule size decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

ovary without detectable defects

yolk amount decreased

mature egg not present

tropharium structure irregular

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


adult lethality

adult nutritional state potentially bad (cachectic)

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality


dorsal ridge row not present

prothorax tracheal opening ectopically present

labium orientation irregular

prothorax potentially transformed

thorax tracheal opening number increased

labium proximal swollen

affected eggs/embryos/larvae

adult lethality

abdomen musculature random partially not present

abdomen musculature specific subset ventral partially rounded

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

tropharium structure irregular

mature egg not present

vitellogenic egg chamber potentially fused

vitellogenic egg chamber position anteriorized

previtellogenic egg chamber not present

vitellarium size decreased

tropharium size decreased

larva number hatched none (< 10 %)

adult lethality

egg production: number <50% of expected

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

larva number hatched many (ca. 70-100 %)


adult lethality


egg production: number <50% of expected


affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

adult lethality

affected eggs/embryos/larvae

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

adult lethality immediately after eclosion


adult lethality

adult lethality

adult lethality


adult lethality

vitellogenic egg chamber number decreased

previtellogenic egg chamber not present

tropharium structure irregular

mature egg not present


adult lethality

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality

affected eggs/embryos/larvae

affected eggs/embryos/larvae

vitellogenic egg chamber late not present

mature egg not present

previtellogenic egg chamber number increased

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality

ovary without detectable defects

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


egg production: number no/almost no eggs

adult lethality


egg production: number >150% of expected


eclosion not fulfilled (died as pupa)


adult lethality

adult lethality


adult lethality

ovary without detectable defects

yolk late amount decreased

egg production: number <50% of expected


adult nutritional state bad (cachectic)

adult lethality

adult lethality


larva segment number decreased

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality

egg production: number <50% of expected


adult lethality


urogomphi not present

mandible not present

abdomen segment number # of remaining segments  decreased

metathorax not present

labium not present

larva segment number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


urogomphi orientation irregular

antenna antennal flagellum size decreased

affected eggs/embryos/larvae

larval musculature fluorescence broader

dead larva

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

eclosion not fulfilled (died as pupa)


adult lethality

affected eggs/embryos/larvae

larval musculature fluorescence decreased

affected eggs/embryos/larvae

tropharium size decreased

previtellogenic egg chamber number decreased

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected


abdomen dorsal not closed

head capsule size decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality


affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)

egg production: number <50% of expected


adult lethality

ovary without detectable defects

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


adult lethality

ovary without detectable defects

larva number hatched many (ca. 70-100 %)

egg production: number <50% of expected

egg production: number <50% of expected


adult lethality

adult lethality immediately after eclosion


adult lethality

affected eggs/embryos/larvae

affected eggs/embryos/larvae


adult lethality

mandible proximal swollen

antenna segment proximal size decreased

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

larva shape irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


larva everted (inside-out)

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

egg colour darker

larva number hatched none (< 10 %)

adult lethality


head capsule size decreased

head appendages position irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

ovary without detectable defects

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality

adult lethality


larva everted (inside-out)

larva segment number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

affected eggs/embryos/larvae

mature egg potentially degenerated

vitellogenic egg chamber number decreased

tropharium structure irregular

mature egg not present


adult lethality

ovary without detectable defects

adult lethality

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality

larva number hatched none (< 10 %)

adult lethality


adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

previtellogenic egg chamber orientation irregular

previtellogenic egg chamber number increased

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality

abdominal odoriferous glands colour darker

adult lethality


adult lethality

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality

hindgut potentially everted (inside-out)

posterior terminus shape irregular

affected eggs/embryos/larvae

larval musculature random not present

terminus musculature potentially size increased

larval musculature random rounded

affected eggs/embryos/larvae

tropharium structure irregular

previtellogenic egg chamber not present

ovary without detectable defects

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected


adult lethality

adult lethality

eclosion not fulfilled (died as pupa)


head capsule size decreased

thorax & abdomen ventral not closed

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

potentially nervous system outside of larva

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

egg production: number <50% of expected

head bristle pattern irregular

head appendages orientation irregular

labium not present

thorax & abdomen segment shape thinner


egg production: number <50% of expected

adult lethality


abdomen segment number decreased

cuticle remnants with unidentifyable segments

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

abdominal odoriferous glands content fragmented

abdominal odoriferous glands content decreased

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


head appendages partially not present

head capsule size decreased

larva everted (inside-out)

affected eggs/embryos/larvae

abdominal odoriferous glands colour lighter

adult lethality

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

eclosion not fulfilled (with pupal and adult features)


mature egg not present

egg chamber mostly not present

vitellarium size decreased

tropharium structure irregular

adult lethality


mature egg not present

ovary without detectable defects

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


follicular epithelium potentially structure irregular

vitellogenic egg chamber amount decreased

mature egg not present

adult lethality


antenna present

head partially everted (inside-out)

labrum not present

larva mostly everted (inside-out)

thorax & abdomen mostly everted (inside-out)

antenna potentially fused

head & thorax fragmented

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


larval musculature fluorescence decreased

affected eggs/embryos/larvae

larva number hatched many (ca. 70-100 %)


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality immediately after eclosion

eclosion not fulfilled (died as pupa)


larva number few (< 50 %) hatched

adult lethality


adult lethality

mature egg not present

yolk late partially amount decreased

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

adult lethality


vitellogenic egg chamber not present

previtellogenic egg chamber not present

mature egg not present

tropharium structure irregular

adult lethality


adult lethality


affected eggs/embryos/larvae

abdominal odoriferous glands size decreased

larval musculature pattern irregular

affected eggs/embryos/larvae

previtellogenic egg chamber amount increased

mature egg not present

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


legs potentially fused

legs partially number increased

affected eggs/embryos/larvae

adult lethality

larval musculature potentially partially not present

larval musculature thinner

larval musculature pattern irregular

affected eggs/embryos/larvae

yolk mostly amount decreased

mature egg not present

tropharium size decreased

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality

vitellogenic egg chamber early amount increased

vitellarium potentially size increased

vitellogenic egg chamber late not present

mature egg not present

egg chamber stage sequence irregular

adult lethality

egg production: number no/almost no eggs


larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

ovary without detectable defects

egg production: number <50% of expected

adult lethality


adult lethality

adult lethality


abdomen tracheal opening size irregular

head capsule size decreased

urogomphi potentially everted (inside-out)

prothorax potentially not present

abdomen segment number decreased

thorax segment number decreased

head appendages unilateral mostly not present

larva segment number decreased

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

tropharium structure irregular

previtellogenic egg chamber not present

vitellogenic egg chamber number decreased

mature egg not present

larva number few (< 50 %) hatched

adult lethality

egg production: number <50% of expected

eclosion not fulfilled (with pupal and adult features)


adult lethality

adult lethality

adult lethality


adult nutritional state potentially bad (cachectic)


head appendages shape irregular

head capsule size decreased

thorax segment number decreased

legs size decreased

legs podomer number decreased

head split

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


larva number few (< 50 %) hatched


adult lethality

adult nutritional state bad (cachectic)

adult lethality

eclosion not fulfilled (died as pupa)


abdominal odoriferous glands central content lighter

abdominal odoriferous glands content fragmented

adult lethality

adult lethality

larva number hatched many (ca. 70-100 %)

egg production: number <50% of expected

adult lethality


abdomen musculature random rounded

prothorax musculature specific subset oblique posterior number decreased

affected eggs/embryos/larvae

mature egg not present

previtellogenic egg chamber stage sequence irregular

previtellogenic egg chamber number increased

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality

tropharium partially structure irregular

egg chamber not present

mature egg not present

adult lethality


larva partially everted (inside-out)

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


larva partially everted (inside-out)

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality immediately after eclosion


adult lethality


abdomen segment number decreased

affected eggs/embryos/larvae

adult lethality


adult lethality

adult lethality

affected eggs/embryos/larvae


adult lethality

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

adult lethality


abdomen segment number decreased

thorax segment number decreased

thorax & abdomen segment number decreased

affected eggs/embryos/larvae

abdominal odoriferous glands partially content darker

abdominal odoriferous glands content spotted

adult lethality

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

eclosion not fulfilled (with pupal and adult features)


affected eggs/embryos/larvae

adult lethality


affected eggs/embryos/larvae

adult lethality

affected eggs/embryos/larvae

yolk late sometimes amount decreased

mature egg not present

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


adult lethality

adult lethality


adult lethality

adult lethality

adult lethality

egg production: number no/almost no eggs

adult lethality

adult lethality


abdomen tracheal opening potentially size decreased

mesothorax tracheal opening potentially size decreased

affected eggs/embryos/larvae

abdominal odoriferous glands content decreased

larva number hatched many (ca. 70-100 %)


adult lethality

head & thorax sometimes not present

labrum sometimes not present

larva sometimes everted (inside-out)

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

adult lethality

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


abdominal odoriferous glands partially colour lighter

abdominal odoriferous glands content fragmented


abdominal odoriferous glands size decreased


mature egg not present

vitellogenic egg chamber late amount increased

yolk late amount decreased

adult lethality

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:


abdominal odoriferous glands content lighter

adult lethality


adult lethality

adult lethality immediately after eclosion


adult lethality


affected eggs/embryos/larvae

adult lethality

adult lethality

egg production: number <50% of expected


adult lethality

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected


adult nutritional state bad (cachectic)

adult lethality

adult lethality


larval musculature potentially dorsal fluorescence decreased

affected eggs/embryos/larvae

adult lethality


abdominal odoriferous glands mostly content not present

abdominal odoriferous glands sometimes content darker


adult lethality

embryo/egg without visible larval cuticle in cuticle preparation

Few empty eggs while remaining shows empty hatched eggs shells.

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

egg production: number no/almost no eggs

adult lethality


larva partially everted (inside-out)

head & thorax & abdomen segment random not present

head & thorax & abdomen segment shape irregular

larva dorsal bent

larva complete everted (inside-out)

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva number hatched many (ca. 70-100 %)


head & thorax & abdomen segment irregular

affected eggs/embryos/larvae

adult lethality


larva segment shape irregular

affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult nutritional state bad (cachectic)

egg production: number <50% of expected


adult lethality

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


head capsule size decreased

head appendages shape irregular

head appendages random not present

Antenna is the most sensitive part

affected eggs/embryos/larvae

adult lethality

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality


head appendages irregular

larva irregular

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality


ovary without detectable defects

mature egg not present

egg chamber complete not present

adult lethality


affected eggs/embryos/larvae


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


legs tibiotarsus proximal division boundary fused

Tibiotarsus and femur fused and shortened.

maxilla size broader

head capsule shape irregular

head dorsal not closed

At the boundary beetween head and thorax the cuticle is either constricted or ruptured.

antenna shape irregular

affected eggs/embryos/larvae

larval musculature random not present

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

abdomen segment sometimes not present

larva sometimes everted (inside-out)

abdomen segment sometimes number decreased

legs sometimes number decreased

affected eggs/embryos/larvae


head & thorax segment shape irregular

affected eggs/embryos/larvae

adult lethality


adult lethality

adult lethality


adult lethality

adult lethality

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larval musculature irregular

affected eggs/embryos/larvae

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult nutritional state potentially bad (cachectic)

adult lethality


head appendages position irregular

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


mature egg not present

previtellogenic egg chamber not present

adult nutritional state bad (cachectic)


mature egg not present

adult lethality

vitellogenic egg chamber late degenerated

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:


adult lethality

adult lethality

adult lethality


mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult nutritional state bad (cachectic)


head appendages position irregular

head & thorax & abdomen irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


vitellogenic egg chamber shape irregular

mature egg not present

vitellogenic egg chamber degenerated

previtellogenic egg chamber not present

egg production: number no/almost no eggs


adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult nutritional state bad (cachectic)


head appendages random not present

head capsule shape irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


head appendages random not present

head & thorax & abdomen segment random not present

head appendages shape irregular

head & thorax & abdomen segment shape irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


head & thorax segment position irregular

affected eggs/embryos/larvae

larva number hatched many (ca. 70-100 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


head & thorax appendages random not present

abdomen segment random constricted

larva segment random irregular

affected eggs/embryos/larvae

adult lethality


affected eggs/embryos/larvae

larval musculature random not present

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


legs pretarsus shape broader

Pretarsus potentially not bent

affected eggs/embryos/larvae

larval musculature irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


vitellogenic egg chamber potentially late fused

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality


head & thorax & abdomen

cuticle remnants strongly fragmented (cuticle crumbs)

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


antenna shape irregular

abdomen shape irregular

posterior terminus present

abdomen partially everted (inside-out)

abdomen segment number decreased

labrum shape irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

adult lethality


adult lethality

adult lethality


head appendages orientation irregular

head shape irregular

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)


adult lethality


larval musculature pattern irregular

affected eggs/embryos/larvae

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

adult lethality


larval musculature pattern irregular

affected eggs/embryos/larvae

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


abdomen musculature specific subset oblique not present

affected eggs/embryos/larvae

adult lethality


adult lethality

egg production: number <50% of expected

adult lethality

vitellogenic egg chamber late colour darker

vitellogenic egg chamber late structure irregular

yolk deposition irregular

larva number hatched none (< 10 %)


adult lethality

previtellogenic egg chamber number decreased

tropharium structure irregular

tropharium size decreased

mature egg not present

adult lethality

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)


larva number hatched none (< 10 %)

adult lethality

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae


posterior terminus not present

prothorax potentially transformed

head appendages sometimes fused

legs shape irregular

legs number increased

metathorax sometimes partially not present

labium potentially transformed

labium position posteriorized

mandible sometimes not present

abdomen segment mostly number decreased

prothorax segment duplicated

antenna sometimes not present

larva segment number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 5 >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

adult lethality

adult lethality

adult lethality


larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


adult lethality

abdomen musculature random rounded

affected eggs/embryos/larvae

adult lethality


larva dorsal not closed

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

adult lethality

affected eggs/embryos/larvae

previtellogenic egg chamber early shape irregular

previtellogenic egg chamber stage sequence irregular

egg chambers appear too big towards the plug

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


adult lethality

larval musculature fluorescence broader

majority of hatched larvae on slide is dead

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


tropharium without detectable defects

vitellarium irregular

mature egg not present

based on observations during preparation

adult lethality


adult lethality

adult lethality

adult lethality


adult lethality


labium not present

head capsule shape irregular

mandible not present

cuticle remnants with unidentifyable segments

abdomen dorsal not closed

head size decreased

cuticle remnants strongly fragmented (cuticle crumbs)

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number few (< 50 %) hatched

egg production: number <50% of expected


affected eggs/embryos/larvae

abdominal odoriferous glands content irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


larval musculature fluorescence decreased

affected eggs/embryos/larvae

adult lethality


adult lethality

vitellogenic egg chamber late structure degenerated

mature egg not present

egg production: number no/almost no eggs

adult lethality


labium shape irregular

labrum size decreased

affected eggs/embryos/larvae

adult lethality

adult lethality


labium shape irregular

labrum size decreased

affected eggs/embryos/larvae

adult lethality

adult lethality

legs partially number decreased

head appendages sometimes not present

head & thorax sometimes partially not present

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae


abdominal odoriferous glands colour darker

adult lethality

eclosion not fulfilled (with pupal and adult features)

head & thorax sometimes not present

abdomen sometimes number decreased

legs sometimes number decreased

larva sometimes everted (inside-out)

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva number hatched many (ca. 70-100 %)

adult lethality


affected eggs/embryos/larvae

adult lethality

larval musculature potentially thinner

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


abdominal odoriferous glands partially colour lighter

abdominal odoriferous glands unilateral colour darker

preparation insufficient

adult lethality


adult lethality

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult lethality


larva segment anterior number decreased

larva dorsal bent

abdomen dorsal not closed

head appendages shape irregular

larva potentially everted (inside-out)

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


labrum slightly size decreased

antenna antennal flagellum unilateral not present

affected eggs/embryos/larvae

adult lethality


larva ventral not closed

legs tibiotarsus potentially fused

with femur

larva appendages shape irregular

legs size decreased

mandible shape irregular

larva segment size decreased

affected eggs/embryos/larvae

larval musculature thinner

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched

adult lethality


eclosion not fulfilled (died as pupa)


antenna proximal size decreased

legs thinner

legs size decreased

head appendages shape irregular

larva partially everted (inside-out)

affected eggs/embryos/larvae

larval musculature pattern irregular

larval musculature fluorescence broader

larvae seem to die some time after hatching

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

adult lethality

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

previtellogenic egg chamber orientation irregular

previtellogenic egg chamber number increased

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult nutritional state potentially bad (cachectic)

adult lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

adult lethality


adult lethality

larva number hatched none (< 10 %)

adult lethality


mandible shape irregular

antenna shape irregular

abdomen cuticle posterior structure irregular

legs potentially size decreased

antenna not present

affected eggs/embryos/larvae

larval musculature fluorescence decreased

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larval musculature ventral thinner

affected eggs/embryos/larvae

mature egg not present

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


dorsal ridge row bristle dorsal present

antenna antennal flagellum not present

head appendages random not present

head appendages shape irregular

head capsule size decreased

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched

adult lethality

adult lethality


posterior terminus shape irregular

abdomen segment number decreased

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


larva number few (< 50 %) hatched

adult lethality


abdominal odoriferous glands content fragmented

adult lethality


adult lethality

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality

adult lethality


adult lethality immediately after eclosion


adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

cuticle remnants strongly fragmented (cuticle crumbs)

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

ovary without detectable defects

previtellogenic egg chamber slightly orientation irregular

mature egg deposition 2 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


thorax partially constricted

mesothoracic tracheal opening size increased

abdomen segment number decreased

head capsule size decreased

head appendages orientation irregular

head appendages shape irregular

antenna mostly not present

larva segment number decreased

abdomen tracheal opening sometimes size increased

legs shape irregular

labrum not present

larva cuticle irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)


adult lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


larva number hatched many (ca. 70-100 %)


head appendages not present

head appendages random shape irregular

head capsule not present

cuticle remnants with unidentifyable segments

abdomen shape irregular

thorax segment number decreased

head capsule size decreased

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality


adult lethality

adult lethality


adult lethality

mature egg not present

egg chamber number decreased

tropharium size decreased

ovary structure irregular

ovary size decreased


labium not present

posterior terminus not present

thorax segment number increased

abdomen segment number decreased

legs number increased

maxilla not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


head & thorax segment shape irregular

head & thorax segment random not present

larva dorsal not closed

larva everted (inside-out)

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched


ovary without detectable defects

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality


gena triplet seta number increased

abdomen segment number decreased

vertex triplet vertex triplet posterior not present

head capsule shape irregular

Especially, Labrum shape is irregular.

abdomen anterior constricted

head antennal flagellum not present

head & thorax not present

affected eggs/embryos/larvae

larval musculature fluorescence decreased

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

egg production: number no/almost no eggs

adult lethality

adult lethality


adult lethality

egg production: number <50% of expected


affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


larva appendages shape swollen

mandible shape irregular

labium shape swollen

legs femur shape swollen

legs tibiotarsus shape swollen

legs tibiotarsus size shortened

legs femur potentially size shortened

head appendages partially shape irregular

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

leg musculature partially not present

larval musculature thinner

head musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


head & thorax & abdomen random not present

affected eggs/embryos/larvae

larval musculature potentially thinner

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

eclosion not fulfilled (with pupal and adult features)


legs random not present

head capsule shape irregular

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

mature egg not present

adult lethality


head & thorax & abdomen segment random not present

larva complete everted (inside-out)

In most of the cases,  hind gut of larvae are outside.

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult nutritional state potentially bad (cachectic)

adult lethality

adult nutritional state bad (cachectic)

adult lethality


adult lethality

affected eggs/embryos/larvae


adult lethality


adult lethality

abdomen constricted

head & thorax & abdomen segment partially random not present

abdomen segment number decreased

legs podomer fused

head & thorax & abdomen segment shape irregular

affected eggs/embryos/larvae

This phenotype is quite varaible. At least all tagmata are present in 50-80% cases & there segment/appendages are randomly absent or their shape is affected.

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality

adult lethality

adult lethality


larva number hatched many (ca. 70-100 %)

adult lethality


affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


legs tibiotarsus size shortened

larva dorsal bent

affected eggs/embryos/larvae

larval musculature random not present

abdomen musculature dorsal pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality


head & thorax random shape irregular

legs segment partially fused

legs shape irregular

Femur & tibiotarsus are fused & metathorasic leg is more affected compare to other legs.

antenna antennal flagellum not present

Only one out of two antenna flagellum is absent.

larva need term

Larva are enclosed in egg cell.

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larval musculature thinner

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

abdomen musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larval musculature pattern irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

previtellogenic egg chamber shape irregular

previtellogenic egg chamber potentially not present

adult lethality


adult lethality

adult lethality

adult lethality


labrum position irregular

labrum complete not present

labrum size decreased

larva everted (inside-out)

affected eggs/embryos/larvae

larval musculature random not present

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva shape irregular

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


cuticle remnants strongly fragmented (cuticle crumbs)

head & thorax & abdomen random partially not present

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

previtellogenic egg chamber not present

previtellogenic egg chamber

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number no/almost no eggs

eclosion not fulfilled (with pupal and adult features)


affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality


Egg shell appears irregular or shrunk.

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality


ovary without detectable defects

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality


adult lethality

adult lethality

adult nutritional state bad (cachectic)


adult nutritional state potentially bad (cachectic)

ovary without detectable defects

adult nutritional state bad (cachectic)


larva number hatched many (ca. 70-100 %)


adult lethality

adult lethality


larva number hatched none (< 10 %)

affected eggs/embryos/larvae


abdomen segment random not present

thorax & abdomen appendages random structure irregular

affected eggs/embryos/larvae

only 4 to 5 larvae's abdomen are affected & in 2 to 3 cases thorax & its legs are affected.

larval musculature random not present

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


larva shape irregular

head & thorax & abdomen segment random not present

larva partially everted (inside-out)

larva complete everted (inside-out)

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva complete everted (inside-out)

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

affected eggs/embryos/larvae

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality


affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


affected eggs/embryos/larvae

adult lethality

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


abdominal odoriferous glands size decreased

abdominal odoriferous glands partially colour lighter

abdominal odoriferous glands content split

adult lethality


abdominal odoriferous glands content decreased

adult lethality

adult lethality


abdominal odoriferous glands potentially content not present

Stink glands were not visible

abdominal odoriferous glands content fragmented

eclosion not fulfilled (with pupal and adult features)


head not present

antenna circumference decreased

labrum orientation irregular

labrum not present

abdomen segment number decreased

thorax segment number decreased

antenna not present

thorax & abdomen segment number decreased

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


larva number hatched many (ca. 70-100 %)

adult lethality


content looks compartimentet

abdominal odoriferous glands partially content lighter

adult lethality

adult lethality


mandible not present

head appendages partially not present

head capsule size decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

labium not present

maxilla not present

dorsal ridge row not present


adult lethality

adult lethality

adult lethality immediately after eclosion


legs size decreased

abdomen segment number decreased

thorax segment number decreased

head capsule size decreased

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


head cuticle lateral ventral constricted

abdomen tracheal opening present

Tracheal system disrupted, but tracheal openings not affected

maxilla bristle position ventralized

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


abdomen musculature random not present

abdomen musculature random rounded

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

adult lethality


abdomen posterior rounded

abdomen dorsal partially not fused

abdomen segment number decreased

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched

adult lethality


adult lethality


affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


head appendages partially not present

mandible not present

abdomen segment number decreased

legs podomer partially not present

head capsule size decreased

affected eggs/embryos/larvae

abdominal odoriferous glands content opaque

abdominal odoriferous glands size decreased

larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


head appendages random not present

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

egg production: number no/almost no eggs

adult lethality

adult lethality


abdomen partially dorsal not fused

head appendages shape irregular

head & thorax not present

abdomen segment number decreased

head capsule size decreased

larva segment number decreased

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


mature egg not present

previtellogenic egg chamber potentially amount increased

vitellogenic egg chamber late amount increased

vitellogenic egg chamber late size decreased

adult lethality

eclosion not fulfilled (with pupal and adult features)


thorax & abdomen segment partially number decreased

thorax & abdomen partially dorsal not fused

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


larva partially everted (inside-out)

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

adult lethality

adult lethality

adult lethality


larva number hatched many (ca. 70-100 %)


legs podomer number decreased

abdomen segment number decreased

thorax segment number decreased

thorax & abdomen segment number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


legs sometimes size decreased

head appendages random partially not present

abdomen segment number decreased

thorax segment number decreased

head capsule size decreased

larva segment number decreased

usually more missing on one side than on the other

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


vitellarium stage sequence irregular

tropharium anterior structure irregular

previtellogenic egg chamber number decreased

egg production: number <50% of expected

adult lethality


larva potentially everted (inside-out)

larva segment potentially number decreased

larva potentially circumference decreased

cuticle remnants with unidentifyable segments

there seems to be an A-P-polarity present

affected eggs/embryos/larvae

abdominal odoriferous glands size decreased

abdominal odoriferous glands colour irregular

abdominal odoriferous glands content split

abdominal odoriferous glands complete content not present

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

previtellogenic egg chamber potentially position irregular

vitellogenic egg chamber not present

vitellarium size decreased

mature egg not present

adult lethality

egg production: number no/almost no eggs

eclosion not fulfilled (with pupal and adult features)


adult lethality


abdomen dorsal partially not fused

abdomen segment number decreased

thorax segment number decreased

head segment number decreased

larva segment number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

adult lethality

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

prothorax musculature specific subset dorsal oblique rounded

prothorax musculature dorsal pattern irregular

affected eggs/embryos/larvae

larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

adult lethality


adult lethality

larval musculature fluorescence decreased

affected eggs/embryos/larvae

mature egg not present

apart from that, without detectable defects

ovary without detectable defects

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

adult lethality


thorax segment thinner

abdomen segment thinner

legs size decreased

head capsule size decreased

thorax & abdomen segment thinner

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

abdominal odoriferous glands content decreased

adult lethality

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)


adult lethality

adult lethality

adult lethality


adult lethality

adult nutritional state

adult lethality

adult lethality


abdomen segment number decreased

thorax segment number decreased

head capsule size decreased

thorax & abdomen segment number decreased

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


abdomen segment number decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


larva circumference decreased

the not everted part as well as the "bent" larvae

larva dorsal bent

larva partially everted (inside-out)

larva complete everted (inside-out)

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


previtellogenic egg chamber sometimes number increased

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

motility decreased

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


tropharium circumference increased

tropharium size decreased

vitellarium size decreased

vitellogenic egg chamber potentially position anteriorized

previtellogenic egg chamber not present

mature egg not present

tropharium structure irregular

adult lethality


antenna pedicellus size decreased

antenna antennal flagellum sometimes not present

gena triplet bristle number increased

affected eggs/embryos/larvae

adult lethality

head musculature specific subset potentially size increased

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality


abdomen segment number decreased

affected eggs/embryos/larvae

abdominal odoriferous glands size decreased

abdominal odoriferous glands content split

larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

eclosion not fulfilled (with pupal and adult features)


adult lethality

adult nutritional state bad (cachectic)

egg production: number no/almost no eggs


head capsule partially not present

head appendages random not present

head & thorax & abdomen appendages random not present

affected eggs/embryos/larvae

larva number hatched many (ca. 70-100 %)

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

adult lethality


affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


posterior terminus shape irregular

posterior terminus not present

abdomen segment shape irregular

abdomen segment random number decreased

cuticle remnants strongly fragmented (cuticle crumbs)

affected eggs/embryos/larvae

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult lethality

adult lethality

adult lethality


vitellarium size decreased

vitellogenic egg chamber number decreased

abdominal odoriferous glands content not present

ovary without detectable defects

previtellogenic egg chamber not present

vitellarium shape irregular

mature egg not present


head capsule size decreased

head appendages random not present

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality

adult lethality


ovariole size decreased

egg chamber potentially degenerated

egg chamber number decreased

mature egg not present


cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


previtellogenic egg chamber potentially not present

egg chamber number decreased

mature egg not present

ovariole size shortened

adult lethality

adult lethality


ovary without detectable defects

mature egg not present

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:


head & thorax & abdomen random not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality


adult nutritional state bad (cachectic)

adult lethality

adult nutritional state bad (cachectic)

adult lethality


abdominal odoriferous glands potentially content not present

abdominal odoriferous glands partially content darker

abdominal odoriferous glands content darker

odoriferous glands colour darker

adult lethality


affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

eclosion not fulfilled (with pupal and adult features)


larval musculature potentially posterior shape irregular

It's same pic which is already used in above annotation.

larval musculature random pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

legs sometimes number decreased

head & thorax mostly not present

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


larva segment random not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

mature egg number increased

egg chamber pattern irregular

vitellogenic egg chamber fused

larva number hatched none (< 10 %)

egg production: number <50% of expected


adult lethality

adult lethality

egg production: number no/almost no eggs


adult lethality

larva number hatched none (< 10 %)


head & thorax & abdomen appendages random not present

head appendages partially not present

head & thorax appendages shape irregular

cuticle remnants strongly fragmented (cuticle crumbs)

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

ovary without detectable defects

adult nutritional state bad (cachectic)


adult lethality

adult lethality

adult lethality


egg production: number <50% of expected


head & thorax appendages random shape irregular

head & thorax & abdomen segment random not present

affected eggs/embryos/larvae

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

larval musculature fluorescence decreased

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

previtellogenic egg chamber not present

mature egg not present

ovary

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:


adult lethality


larva segment random not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


larva segment random not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

adult lethality

adult lethality

adult lethality

adult lethality


adult lethality

adult lethality


abdominal odoriferous glands potentially content not present

abdominal odoriferous glands content darker

vitellarium size shortened

egg chamber number decreased

vitellarium degenerated

In 3 cases,  morphology of egg chamber are irregular, & probably going to be disappear. But in one case, all egg chamber are completely absent.

vitellarium thinner

mature egg not present

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

larval musculature random potentially not present

larval musculature thinner

affected eggs/embryos/larvae

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


pygopodium potentially ectopically structure present

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

adult nutritional state bad (cachectic)


larva segment central constricted

Constriction of abdomen segment is major phenotype but with this phenotype there is also head & thorax appendages shape irregular & larvae segements are randomly partially not present in all three tagmata.

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


larva number hatched many (ca. 70-100 %)

adult lethality


head & thorax & abdomen segment shape irregular

cuticle remnants with some identifiable segments

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larval musculature random not present

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

larval musculature thinner

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality

adult lethality


larval musculature pattern irregular

affected eggs/embryos/larvae


larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality


larva cuticle dorsal irregular

head appendages shape irregular

abdomen segment potentially number decreased

legs number increased

head appendages partially random not present

larva appendages number irregular

cuticle remnants with unidentifyable segments

affected eggs/embryos/larvae

adult lethality

larval musculature fluorescence decreased

larval musculature pattern irregular

affected eggs/embryos/larvae

previtellogenic egg chamber potentially number increased

yolk late amount decreased

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality

adult lethality


vitellogenic egg chamber orientation irregular

previtellogenic egg chamber number increased

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

adult lethality

egg production: number <50% of expected

adult lethality


cuticle remnants strongly fragmented (cuticle crumbs)

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


larva number hatched many (ca. 70-100 %)


labrum size decreased

dorsal ridge row dorsal not present

head appendages random shape irregular

abdomen segment number decreased

antenna not present

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult lethality

adult lethality


adult lethality

adult lethality


thorax cuticle irregular

thorax segment number decreased

head potentially size decreased

thorax constricted

head appendages shape irregular

head & thorax segment number decreased

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

adult lethality

adult lethality


just 1

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

mature egg potentially deposition irregular

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


just 1

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


abdominal odoriferous glands partially content opaque

abdominal odoriferous glands content split


head appendages shape irregular

legs shortened

abdomen shape irregular

legs podomer potentially fused

head capsule size decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


affected eggs/embryos/larvae

previtellogenic egg chamber orientation irregular

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality


abdominal odoriferous glands content fragmented

adult lethality

adult lethality


affected eggs/embryos/larvae

adult lethality

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality

adult lethality


affected eggs/embryos/larvae

abdominal odoriferous glands content irregular

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality


mandible potentially transformed

labrum not present

maxilla number increased

mandible not present

3 / 5 larvae on slide

affected eggs/embryos/larvae

adult lethality

egg production: number <50% of expected

adult lethality


affected eggs/embryos/larvae

adult lethality


adult lethality

previtellogenic egg chamber sometimes potentially number increased

yolk late deposition irregular

vitellogenic egg chamber late structure degenerated

mature egg number decreased

larva number hatched none (< 10 %)

adult lethality


adult lethality

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (with pupal and adult features)


affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

egg chamber not present

tropharium potentially structure degenerated

mature egg shape irregular

vitellogenic egg chamber late not present

mature egg not present

larva number hatched none (< 10 %)

adult lethality


larva number hatched many (ca. 70-100 %)

adult lethality

adult lethality immediately after eclosion


larva partially everted (inside-out)

antenna not present

larva everted (inside-out)

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


abdomen size decreased

mesothorax structure irregular

head appendages shape irregular

head capsule size decreased

larva segment number decreased

abdomen cuticle structure irregular

metathorax not present

antenna not present

larva fragmented

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult lethality

adult lethality

adult lethality


previtellogenic egg chamber potentially number increased

vitellogenic egg chamber late sometimes structure degenerated

adult lethality

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality


embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

adult lethality

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)

adult lethality


adult lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

adult lethality

adult lethality


adult lethality

vitellarium size decreased

vitellogenic egg chamber stage sequence irregular

egg chamber number decreased

mature egg not present

adult lethality


affected eggs/embryos/larvae

abdominal odoriferous glands partially content darker

adult lethality

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


adult lethality


head posterior potentially constricted

head cuticle ventral posterior structure irregular

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

ovary without detectable defects

mature egg not present

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


head appendages potentially partially fused

labium potentially transformed

into leg like structure. Just one embryo on slide

legs pretarsus number increased

maxilla potentially everted (inside-out)

head capsule shape irregular

antenna not present

cuticle remnants with some identifiable segments

legs sometimes everted (inside-out)

labium not present

cuticle remnants strongly fragmented (cuticle crumbs)

affected eggs/embryos/larvae

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

eclosion not fulfilled (with pupal and adult features)


head not present

antenna shape irregular

antenna not present

antenna not present

labrum not present

mandible not present

labrum orientation irregular

cuticle remnants with unidentifyable segments

labrum size decreased

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality

adult lethality


larva number hatched many (ca. 70-100 %)

adult lethality


maxilla sometimes not present

antenna sometimes not present

mandible sometimes not present

labium not present

labrum shape irregular

head appendages random not present

legs number decreased

head capsule size decreased

head & thorax potentially partially fused

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

previtellogenic egg chamber orientation irregular

yolk sometimes deposition irregular

previtellogenic egg chamber number increased

adult lethality

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality


thorax mostly not present

abdomen segment number decreased

head capsule structure irregular

head & thorax & abdomen constricted

abdomen anterior constricted

thorax posterior constricted

head split

larva fragmented

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larval musculature pattern irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


posterior terminus potentially not present

abdomen cuticle shape irregular

labrum size decreased

abdomen segment potentially number decreased

head appendages orientation irregular

larva partially everted (inside-out)

affected eggs/embryos/larvae

adult lethality

larval musculature thinner

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched


adult lethality

head & thorax & abdomen segment random not present

head capsule shape irregular

head & thorax & abdomen segment shape irregular

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

larva number hatched none (< 10 %)


head capsule shape irregular

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva fragmented

procephalic head mostly not present

head appendages position irregular

head capsule size decreased

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


head appendages position irregular

abdomen constricted

head capsule not present

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)


legs segment potentially fused

legs size shortened

affected eggs/embryos/larvae

larval musculature random fluorescence not present

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult lethality


adult lethality

adult lethality

adult lethality

adult lethality


vitellarium potentially size shortened

mature egg not present

egg chamber mostly not present

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


abdominal odoriferous glands colour decreased

ovary without detectable defects

egg chamber potentially fused

mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality


ovary without detectable defects

egg chamber potentially fused

adult lethality


adult lethality

larval musculature fluorescence decreased

affected eggs/embryos/larvae


adult lethality

eclosion not fulfilled (with pupal and adult features)


cuticle remnants with some identifiable segments

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality


adult lethality

head & thorax & abdomen appendages partially random not present

head mostly not present

head & thorax appendages partially random not present

affected eggs/embryos/larvae

egg production: number no/almost no eggs


ovary without detectable defects

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected


head appendages shape irregular

antenna shape irregular

legs size shortened

Femur and tibiotarsus are potentially fused

labrum not present

affected eggs/embryos/larvae

larval musculature random pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


tropharium structure irregular

vitellarium potentially size shortened

mature egg not present

egg chamber mostly not present


gena triplet bristle potentially pattern irregular

head appendages potentially position irregular

head capsule potentially shape irregular

affected eggs/embryos/larvae

adult lethality

larva number hatched none (< 10 %)

adult lethality


mature egg not present

egg chamber mostly not present

adult lethality


head appendages shape irregular

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected


adult lethality

adult nutritional state bad (cachectic)

adult lethality


head & thorax & abdomen segment random not present

head & thorax & abdomen segment shape irregular

affected eggs/embryos/larvae

embryo/egg with embryonic tissue, no cuticle, no fluorescence

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


affected eggs/embryos/larvae


affected eggs/embryos/larvae

larval musculature random irregular

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


head appendages shape irregular

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

adult lethality


affected eggs/embryos/larvae

adult lethality


vitellogenic egg chamber late degenerated

mature egg not present

adult nutritional state bad (cachectic)

adult lethality


larva number hatched many (ca. 70-100 %)


abdominal odoriferous glands content darker

mature egg not present

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number no/almost no eggs

adult lethality


adult lethality

adult nutritional state bad (cachectic)


larva dorsal not closed

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality


mature egg not present


head capsule shape irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


mandible potentially shape irregular

labrum not fused

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality


head appendages shape irregular

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality


head appendages random not present

head capsule not present

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva complete everted (inside-out)

larva dorsal not closed

affected eggs/embryos/larvae

larval musculature pattern irregular

larval musculature random not present

affected eggs/embryos/larvae

larva number few (< 50 %) hatched

adult lethality