eclosion delayed (still pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

legs segment potentially fused

abdomen circumference increased

abdomen bent

abdomen circumference decreased

legs segment shortened

head capsule size decreased

larva shape irregular

larval musculature irregular

larval musculature mostly fluorescence not present

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number few (< 50 %) hatched


eclosion not fulfilled (with pupal and adult features)

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality

larva number hatched many (ca. 70-100 %)

pupa eclosion not fulfilled (died as pupa)


pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult nutritional state

egg production: number no/almost no eggs


pupal molt not fulfilled (died as prepupa)

eclosion not fulfilled (died as pupa)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still prepupa)

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality

adult lethality


eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

oblique dorsal thoracic muscles shortened

pupal molt not fulfilled (died as pupa)

longitudinal dorsal thoracic muscles position irregular

wings shape irregular

pupal molt delayed (still larva)

larva lethality

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


larva lethality


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality

adult lethality

adult nutritional state bad (cachectic)


adult lethality

vitellogenic egg chamber number decreased

previtellogenic egg chamber not present

tropharium shape irregular

adult lethality

larva lethality

abdomen segment number decreased

abdomen complete everted (inside-out)

legs segment shortened

larva cuticle dorsal not closed

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg with embryonic tissue, no cuticle, no fluorescence

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larva number few (< 50 %) hatched


adult lethality

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)


larva lethality

adult lethality

adult lethality

adult lethality


abdominal odoriferous glands irregular

tropharium shortened

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)


previtellogenic egg chamber not present

egg production: number <50% of expected

eclosion not fulfilled (died as pupa)

adult lethality

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

prothorax not present

head capsule complete not present

thorax cuticle dorsal irregular

head capsule mostly not present

larval musculature fluorescence decreased

larva number few (< 50 %) hatched


larva lethality

adult lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)


eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality


adult lethality

eclosion delayed (still pupa)

larva lethality

antenna random not present

head appendages sometimes random not present

head appendages shape irregular

prothoracic leg not present

abdomen segment number decreased

thorax cuticle dorsal irregular

head capsule size decreased

head & thorax cuticle dorsal irregular

larval musculature irregular

larva number few (< 50 %) hatched


pupal molt delayed (still larva)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

cuticle remnants with some identifiable segments

appendages random remaining

larval musculature irregular

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

legs number increased

labium not present

abdomen segment number decreased

maxilla not present

affected eggs/embryos/larvae

adult lethality


previtellogenic egg chamber not present

mature egg not present

vitellarium size decreased

egg production: number <50% of expected

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

maxillary palp orientation irregular

maxillary palp shape irregular


adult lethality

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

larva lethality

vertex triplet irregular

head capsule mostly not present

larva cuticle dorsal irregular

larval musculature mostly fluorescence not present

larval musculature shape irregular

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number hatched many (ca. 70-100 %)


larva lethality

adult lethality

adult lethality

eclosion not fulfilled (with pupal and adult features)


eclosion not fulfilled (not completely eclosed)

pupal molt delayed (still prepupa)

larva lethality

abdomen segment number decreased

thorax & abdomen segment number decreased

appendages partially remaining

larval musculature irregular


mature egg deposition 4 >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

larva lethality


vitellarium complete not present

egg production: number no/almost no eggs

tropharium shortened

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality


vitellarium mostly decreased

tropharium mostly shortened

larval musculature irregular


adult lethality

adult lethality

adult lethality

abdomen partially not present

head & thorax & abdomen partially not present

larval musculature irregular


fertility after larval injection decreased (no living offspring)

adult lethality

larva lethality

adult lethality


adult lethality

adult lethality

larva lethality

abdomen segment number decreased

thorax segment number decreased

head segment sometimes not present

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


ovary thinner

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

larva lethality early (L5/6)

affected eggs/embryos/larvae

affected eggs/embryos/larvae

head slightly posterior segment boundary irregular

larval musculature fluorescence degenerated


adult lethality

larva lethality early (L5/6)

adult lethality


adult lethality

larva lethality early (L5/6)

affected eggs/embryos/larvae

larva appendages irregular


larva lethality early (L5/6)


larva lethality early (L5/6)

adult lethality

adult lethality


adult lethality

larva lethality early (L5/6)


adult lethality

larva lethality early (L5/6)

affected eggs/embryos/larvae

affected eggs/embryos/larvae

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

adult lethality

adult lethality


larva lethality late (L7)

larva lethality early (L5/6)

adult lethality

adult lethality


adult lethality

eclosion delayed (still pupa)

larva lethality early (L5/6)


larva lethality late (L7)

pupal molt delayed (still larva)

pupal molt not fulfilled (died as prepupa)

larva lethality

adult lethality

adult lethality

adult lethality


adult lethality


not fulfilled (died as prepupa)

pupal molt delayed (still prepupa)

pupal molt delayed (still prepupa)

vitellogenic egg chamber late not present

adult lethality


ovary without detectable defects

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality

larva lethality early (L5/6)

larva number hatched many (ca. 70-100 %)

adult lethality

adult lethality


larva lethality early (L5/6)

eclosion not fulfilled (with pupal and adult features)


adult lethality

larva lethality


larva lethality early (L5/6)

adult lethality

adult lethality

adult lethality


larva lethality early (L5/6)

affected eggs/embryos/larvae

affected eggs/embryos/larvae

gena triplet seta number increased

head & thorax & abdomen bristle pattern irregular

larval musculature fluorescence decreased

adult lethality


adult lethality

larva lethality early (L5/6)

adult lethality


larva lethality early (L5/6)

adult lethality

larva number hatched none (< 10 %)


abdominal odoriferous glands colour darker

adult lethality

larva lethality early (L5/6)

affected eggs/embryos/larvae

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


ovariole mostly decreased

larva lethality early (L5/6)

larva partially everted (inside-out)

abdomen partially not present

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larval musculature pattern irregular

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched


adult lethality

adult lethality

pupal molt delayed (still larva)

affected eggs/embryos/larvae

head anterior not present

embryo/egg not developed/not fertilized

larval musculature pattern irregular

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

larva number few (< 50 %) hatched


eclosion not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

egg chamber potentially fused

egg chamber segmentation/division not present

egg production: number no/almost no eggs

adult lethality


adult lethality

larva lethality early (L5/6)

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


adult lethality

wings not closed

eclosion not fulfilled (died as pupa)

wings slightly shortened

larva lethality early (L5/6)

affected eggs/embryos/larvae

larva dorsal not closed

legs shape irregular

larva appendages irregular

legs shortened

larva appendages shortened

vitellogenic egg chamber late number increased

mature egg deposition 4 5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


adult lethality

wings not closed

adult lethality

delayed (still larva)

wings blistered

wings shortened

pupal molt not fulfilled (died as prepupa)

larva lethality early (L5/6)

previtellogenic egg chamber not present


adult lethality

pupal molt delayed (still larva)

pupal molt delayed (still larva)

wings blistered

wings shortened

pupal molt not fulfilled (died as pupa)

larva lethality early (L5/6)


larva lethality early (L5/6)

larval musculature partially spotted

larval musculature partially not present

larva number few (< 50 %) hatched

adult lethality


larva lethality early (L5/6)

affected eggs/embryos/larvae

increased amount of presumably dead larvae shortly before/after hatching, without muscle pattern but diffuse fluorescence

larval musculature not present

larva number few (< 50 %) hatched

adult lethality


adult lethality

larva lethality early (L5/6)


adult lethality

larva lethality early (L5/6)

larva lethality

adult lethality

legs pretarsus shortened

legs tibiotarsus shortened

legs pretarsus broader

legs tibiotarsus broader

legs femur shortened

pygopodium shortened

pygopodium broader

urogomphi shortened

mandible shape irregular

antenna shortened

urogomphi broader

legs femur broader

antenna broader

larva appendages mostly irregular

affected eggs/embryos/larvae

ovary without detectable defects

mature egg deposition 3 >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


larva lethality early (L5/6)

adult lethality

adult lethality


ovary without detectable defects

egg production: number <50% of expected

adult lethality

larva lethality early (L5/6)

larva number hatched many (ca. 70-100 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)

pupa eclosion not fulfilled (died as pupa)


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

adult lethality

adult lethality

adult lethality


adult lethality

larva lethality early (L5/6)

adult lethality


larva lethality late (L7)

mature egg deposition 5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

mature egg deposition 4 >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

egg production: number <50% of expected


adult lethality

adult lethality

ovary without detectable defects

egg production: number <50% of expected

adult lethality

eclosion delayed (still pupa)

affected eggs/embryos/larvae

thorax & abdomen bristle partially not present

thorax & abdomen bristle dorsal pattern irregular

dorsal cuticle seems to be slightly invaginated, causing loss of some bristles.

larval musculature spotted

larval musculature not present

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number few (< 50 %) hatched

About 50-60% hatch rate

adult lethality


larva lethality early (L5/6)

adult lethality

adult lethality

adult lethality


larva lethality

cuticle remnants without visible segmentation, differentiation or axis

abdomen partially not present

abdomen partially remaining

legs sometimes remaining

cuticle remnants with some identifiable segments

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

larval musculature pattern irregular

larval musculature fluorescence decreased

larva number few (< 50 %) hatched


pupal molt not fulfilled (died as L7 or prepupa)

delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

adult lethality

adult nutritional state bad (cachectic)

egg production: number no/almost no eggs

adult lethality

adult lethality


adult lethality

pupal molt delayed (still larva)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality early (L5/6)

mature egg deposition 2 >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

mature egg deposition 3 5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

mature egg deposition 2 5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

larva lethality early (L5/6)

larva number hatched many (ca. 70-100 %)


larva lethality

antenna flagellum not present

legs podomer partially fused

antenna shortened

larva appendages irregular

with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

larval musculature potentially degenerated

embryo/egg with embryonic tissue, no cuticle, no fluorescence

very weak muscle flourescence was coming up in some of those embryos

larva number few (< 50 %) hatched

About 50% probably, not easy to score


pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

egg chamber number decreased

vitellogenic egg chamber late not present

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

adult lethality

adult nutritional state bad (cachectic)

adult lethality

adult lethality

pupa eclosion not fulfilled (died as pupa)


larva lethality early (L5/6)

affected eggs/embryos/larvae

larva cuticle thinner

larva cuticle mostly not present

Cuticle almost not visible.

Cuticle almost not visible.

larval musculature not present

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number hatched none (< 10 %)

adult lethality


larva lethality early (L5/6)

adult lethality


fertility after larval injection decreased (no living offspring)

adult lethality

larva lethality early (L5/6)

adult lethality


ovary without detectable defects

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

hindwing irregular

larva lethality early (L5/6)

affected eggs/embryos/larvae

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

with embryonic tissue, no cuticle, but muscle fluorescence

embryo/egg not developed/not fertilized

larval musculature pattern irregular

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

mature egg deposition 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

mature egg deposition 2 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

mature egg deposition 5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality early (L5/6)

affected eggs/embryos/larvae

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)


egg production: number no/almost no eggs

wings not closed

wings slightly shortened

affected eggs/embryos/larvae

affected eggs/embryos/larvae

gena triplet seta number increased

abdomen bristle sometimes number increased

head bristle pattern irregular

larva number few (< 50 %) hatched

adult lethality


larva lethality early (L5/6)

larva number hatched many (ca. 70-100 %)


larva lethality early (L5/6)


adult lethality

larva lethality early (L5/6)

legs shortened

abdomen partially not present

legs irregular

larva appendages irregular


not fulfilled (died as pupa)

larva lethality

cuticle remnants without visible segmentation, differentiation or axis

legs sometimes remaining

head & thorax & abdomen partially not present

cuticle remnants with some identifiable segments

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg not developed/not fertilized

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larva number hatched none (< 10 %)

adult lethality

adult lethality


adult lethality

larva lethality early (L5/6)

affected eggs/embryos/larvae

thoracic segment sometimes remaining

head partially remaining

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larval musculature pattern irregular

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


larva lethality early (L5/6)

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

head & thorax partially not present

head partially not present

not developed/not fertilized

larval musculature pattern irregular

larva number few (< 50 %) hatched

adult lethality


larva lethality early (L5/6)

head & thorax partially not present

head partially not present

not developed/not fertilized

larval musculature pattern irregular

larva number few (< 50 %) hatched

adult lethality


larva lethality early (L5/6)

eclosion not fulfilled (died as pupa)

larva lethality

larva lethality

larva lethality early (L5/6)

head & thorax partially not present

head partially not present

not developed/not fertilized

larval musculature pattern irregular

larva number few (< 50 %) hatched

adult lethality

adult lethality

pupa eclosion not fulfilled (died as pupa)

adult lethality

adult lethality

adult lethality


larva lethality early (L5/6)

affected eggs/embryos/larvae

procephalic head partially not present

larval musculature pattern irregular


larva lethality early (L5/6)

affected eggs/embryos/larvae


adult lethality

larva lethality early (L5/6)

adult lethality


adult lethality

larva lethality early (L5/6)


adult lethality

adult lethality

larva lethality early (L5/6)

adult lethality


larva lethality early (L5/6)

affected eggs/embryos/larvae

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


larva lethality early (L5/6)

adult lethality


adult lethality

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

tropharium posterior structure irregular

tropharium potentially size increased

egg chamber not present

adult lethality


adult lethality

adult lethality

wings not closed

wings shortened

antenna shortened

larva lethality early (L5/6)

affected eggs/embryos/larvae

abdomen partially # of remaining segments  not present

legs shortened

embryo/egg not developed/not fertilized

larval musculature pattern irregular

slightly above 50%

adult lethality


adult lethality

larva lethality early (L5/6)

affected eggs/embryos/larvae

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


larva lethality late (L7)

larva lethality early (L5/6)

adult lethality

adult lethality

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

larva number hatched none (< 10 %)

adult lethality


larva lethality early (L5/6)


larva lethality


pupal molt not fulfilled (died with pupal and larval features)

pupal molt delayed (still larva)

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)


pupal molt delayed (still larva)

adult lethality

pupal molt delayed (still larva)

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

larva lethality

affected eggs/embryos/larvae

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality

adult lethality


larva lethality

larva number hatched none (< 10 %)

adult lethality immediately after eclosion


egg shell potentially surface irregular

eggs are not covered with flour as much usual

ovary without detectable defects

mature egg deposition 2 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected


tropharium slightly size decreased

tropharium structure irregular

vitellogenic egg chamber not present

ovary without detectable defects

egg production: number <50% of expected

larva lethality

adult lethality

adult lethality

adult lethality


eclosion not fulfilled (not completely eclosed)

wings not closed

wings shape irregular

pupal molt delayed (still larva)

affected eggs/embryos/larvae

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

eclosion not fulfilled (with pupal and adult features)

adult lethality

adult lethality


pupal molt delayed (still larva)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

vitellogenic egg chamber late not present

ovary size decreased

adult lethality

adult nutritional state potentially bad (cachectic)

egg production: number no/almost no eggs


larva lethality

adult lethality

adult lethality


wings not closed

adult lethality

eclosion not fulfilled (died as pupa)

pupal molt not completely molted

larva number hatched many (ca. 70-100 %)


pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still larva)

larva lethality

tropharium degenerated

egg chamber complete not present

adult lethality

adult lethality


abdominal odoriferous glands colour not present

abdominal odoriferous glands shape irregular

ovary size decreased

abdominal odoriferous glands size decreased

ovariole not present

ovary size decreased

adult lethality


pupal molt delayed (still larva)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

pupa eclosion not fulfilled (died as pupa)


eclosion delayed (still pupa)

wings slightly shortened

head slightly orientation irregular

ovary without detectable defects

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected


pronotum shape irregular

elytra proximal blistered

legs orientation irregular

legs shortened

elytra partially blistered

eyes surface irregular

the covering cuticle seems to be more prominent

pupal molt delayed (still larva)

affected eggs/embryos/larvae

larval musculature mostly central thinner

affected eggs/embryos/larvae

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

larva number hatched none (< 10 %)

adult lethality


larva lethality late (L7)

larva lethality late (L7)

adult lethality

adult lethality

adult lethality


elytra proximal blistered

pupal molt delayed (still larva)

larva number few (< 50 %) hatched


larva lethality

adult lethality


prothorax slightly dorsoventral-axis increased

mesoepimeron shape irregular

intercoxal process T3 shape irregular

mesothorax shape irregular

thorax & abdomen slightly thinner


larva lethality late (L7)

pupal molt delayed (still larva)

adult lethality

adult lethality

adult lethality


larva lethality

adult lethality immediately after eclosion


eclosion delayed (still pupa)

adult lethality


eclosion not fulfilled (died as pupa)

pupal molt not completely molted

abdomen gin-traps orientation irregular

eclosion not fulfilled (died as pupa)

abdomen circumference increased

dorsal thoracic muscles not present

wings blistered

ovary without detectable defects

larva number hatched

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (with pupal and adult features)


antenna potentially robustness decreased

larva lethality


ovary without detectable defects

legs tarsus potentially necrotic

legs tarsus partially colour lighter

legs tarsus partially colour darker

adult lethality


mesothoracic leg tarsus shortened

prothoracic leg tarsus shortened

flagellomeres number decreased

antenna shortened

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality

egg chamber complete not present

adult lethality


larva lethality


flagellomeres partially fused

prothoracic leg shortened

flagellomeres number decreased

maxillary palp size increased

legs orientation irregular

antenna distal broader

wings shape irregular

antenna shortened

pupa anteroposterior-axis not bent

affected eggs/embryos/larvae

antenna not present

labrum not present

mandible not present

larva appendages sometimes shape irregular

Head appendages sometimes un-identifiable.

larva cuticle dorsal decreased

cuticle remnants with some identifiable segments

head mostly decreased

cuticle everted (inside-out)

cuticle remnants without visible segmentation but polarity

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


larva lethality

egg production: number no/almost no eggs

adult lethality

adult lethality


adult lethality

wings not closed

elytra blistered

adult appendages distal colour decreased

eclosion not fulfilled (not completely eclosed)

larval musculature coxal musculature partially not present

larval musculature partially not present

larva number hatched none (< 10 %)

adult lethality


adult lethality

larva lethality

larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

wings not closed

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

affected eggs/embryos/larvae

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

larva number few (< 50 %) hatched

adult lethality

adult lethality


pupa anteroposterior-axis irregular

pupal molt not completely molted

larva segment number decreased

thorax & abdomen segment shape irregular

thorax & abdomen segment number decreased

vitellogenic egg chamber late number increased

egg production: number <50% of expected


egg production: number <50% of expected

adult lethality

ovary without detectable defects

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

adult lethality


tropharium structure irregular

previtellogenic egg chamber potentially number increased

vitellogenic egg chamber late not present

ovariole shape irregular

egg production: number <50% of expected

adult lethality

tropharium irregular

vitellogenic egg chamber shape rounded

vitellogenic egg chamber size decreased

previtellogenic egg chamber complete not present

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality


tropharium structure irregular

ovariole shape irregular

egg production: number <50% of expected

adult lethality

tropharium irregular

vitellogenic egg chamber shape rounded

vitellogenic egg chamber size decreased

adult lethality

adult lethality

egg production: number <50% of expected

adult lethality


pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality

adult lethality


tropharium slightly size decreased

egg production: number <50% of expected

larva lethality

affected eggs/embryos/larvae

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

larva number hatched none (< 10 %)


cuticle remnants without visible segmentation but dv-polarity

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

with embryonic tissue, no cuticle, but muscle fluorescence

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number hatched none (< 10 %)


larva lethality late (L7)

pupal molt not fulfilled (died as pupa)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still pupa)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality

ovary without detectable defects

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number no/almost no eggs

adult lethality


larva lethality

adult lethality


pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still larva)

delayed (still prepupa)

legs orientation irregular

eclosion not fulfilled (died as pupa)

wings size decreased

pupal molt delayed (still larva)

larva lethality

ovary size decreased

vitellogenic egg chamber complete not present

adult lethality


eclosion not fulfilled (not completely eclosed)

wings shape irregular

wings slightly shortened

egg chamber mostly not present

adult lethality


mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

pupal molt not fulfilled (died as pupa)

eclosion delayed (still pupa)

eclosion not fulfilled (died as pupa)

with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larva number hatched none (< 10 %)

adult lethality


ovary without detectable defects

adult lethality

larva lethality

larva number hatched many (ca. 70-100 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality

adult lethality

adult lethality

adult lethality


larva lethality


pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

larva lethality

vitellogenic egg chamber mostly not present

previtellogenic egg chamber complete not present

adult lethality

adult lethality


ovary without detectable defects

egg production: number <50% of expected

adult nutritional state bad (cachectic)

abdominal segment 7 sternum slightly surface irregular

larva lethality

larva number few (< 50 %) hatched

adult lethality


adult lethality

dorsal thoracic muscles partially not present

abdomen segment number decreased

posterior terminus shape irregular

abdomen bristle pattern irregular

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number few (< 50 %) hatched


pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality


elytra shape irregular

head ventral surface irregular

adult lethality

pupa eclosion not fulfilled (died as pupa)


adult lethality

flagellomeres distal shape irregular

flagellomeres number decreased

metathoracic leg tibia bent

legs tibia shortened

legs femur shortened

legs shortened

wings shape irregular

wings shortened

antenna shortened

eclosion not fulfilled (died as pupa)

legs orientation irregular

wings orientation irregular

wings shortened

antenna shortened

pupa size decreased

pupal molt delayed (still larva)

vitellogenic egg chamber late degenerated

ovary size decreased

egg chamber complete not present

egg production: number no/almost no eggs

adult lethality


pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

larva lethality late (L7)

pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still larva)

egg chamber mostly not present

adult nutritional state potentially bad (cachectic)

larva number hatched none (< 10 %)

adult nutritional state bad (cachectic)

prothoracic odoriferous glands darker

adult lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)


pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

larva lethality late (L7)

pupal molt delayed (still larva)

egg chamber mostly not present

larva number hatched none (< 10 %)

adult nutritional state bad (cachectic)

prothoracic odoriferous glands darker

adult lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)


adult lethality

elytra slightly blistered

elytra not closed

adult lethality

pupal molt delayed (still pupa)

larva lethality early (L5/6)

abdomen partially everted (inside-out)

abdomen segment partially not present

abdomen dorsoventral-axis shortened

larval musculature pattern irregular

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched

adult lethality


wings shortened

pupal molt delayed (still larva)

affected eggs/embryos/larvae

larva dorsal not closed

larva appendages shape irregular

Phenotype is identical to the phenotype of iB1172

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larval musculature irregular

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected

adult lethality


larva lethality

egg chamber mostly not present

adult lethality


larva lethality

head sometimes partially remaining

abdomen segment number decreased

thorax & abdomen partially not present

thorax sometimes partially remaining

cuticle remnants with some identifiable segments

with embryonic tissue, no cuticle, no fluorescence

larval musculature partially not present

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larva number hatched many (ca. 70-100 %)


larva lethality

adult lethality


eclosion not fulfilled (with pupal and adult features)

one animal developed adult structures still inside the larval cuticle, pupal cuticular structures (gin traps) are visible as well

pupal molt delayed (still larva)

larva lethality

egg chamber number decreased

ovary without detectable defects

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

antenna orientation irregular

pupal molt not fulfilled (died with pupal and larval features)

head orientation irregular

pupal molt delayed (still larva)

adult lethality


oblique dorsal thoracic muscles median slightly elongated

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


wings potentially shortened

wings potentially blistered

animals are dead for too long to make definitive statements and wings are clotted with flour

legs tarsus shape irregular

head orientation irregular

wings orientation irregular

wings shortened

elytra necrotic

eclosion not fulfilled (died as pupa)

elytra blistered

labrum size decreased

affected eggs/embryos/larvae

larva appendages shortened

larva appendages shape irregular

larva partially dorsal not closed

head shape irregular

embryo/egg not developed/not fertilized

larval musculature irregular

vitellogenic egg chamber late number increased

egg chamber segmentation/division irregular

Contact between mid stage egg chambers is potentially to large. Maybe due to missing stalk cells?

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


previtellogenic egg chamber not present

tropharium size decreased

vitellarium not present

motility decreased

ovary without detectable defects

elytra slightly surface irregular

larva lethality

larval musculature fluorescence decreased

larval musculature not present

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

egg chamber slightly number decreased

ovary without detectable defects

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected

pupa eclosion not fulfilled (died as pupa)


larva lethality

larva number few (< 50 %) hatched

about 40-50%

adult lethality


fertility after larval injection decreased (no living offspring)

affected eggs/embryos/larvae

larval musculature not present

Many hatched, dead animals with diffuse green fluorescence. They presumably died directly after hatching. Most unhatched larvae show normal muscle fluorescence.

larva number hatched none (< 10 %)

adult lethality


adult lethality immediately after eclosion

eclosion delayed (still pupa)

head partially anterior not present

head & thorax & abdomen partially not present

cuticle random remaining

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larval musculature irregular

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched

adult lethality


larva lethality late (L7)

larva lethality early (L5/6)

adult lethality

egg production: number no/almost no eggs

adult lethality

adult lethality


oblique dorsal thoracic muscles need term anterior shape irregular

metathoracic leg musculature pl1-tr1 potentially shortened

larval musculature partially not present

larval musculature shape thinner

mesothoracic leg musculature pl1-tr1 potentially shortened

larval musculature pattern irregular

larva number hatched many (ca. 70-100 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


eclosion not fulfilled (died as pupa)

eclosion not fulfilled (died as pupa)

pupal molt not fulfilled (died with pupal and larval features)

pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still larva)

affected eggs/embryos/larvae

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

ovary without detectable defects

egg chamber number decreased

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected


eclosion not fulfilled (died as pupa)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

affected eggs/embryos/larvae

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

ovary without detectable defects

egg chamber number decreased

egg production: number <50% of expected

larva number hatched none (< 10 %)


pupal molt not fulfilled (died as L7 or prepupa)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

tropharium shortened

tropharium shape irregular

egg chamber potentially ectopically present

egg chamber potentially sometimes fused

vitellarium shortened

egg production: number <50% of expected

adult lethality

pupa eclosion not fulfilled (died as pupa)


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

tropharium shortened

tropharium shape irregular

egg chamber potentially sometimes fused

vitellarium shortened

egg production: number <50% of expected

adult lethality


eclosion

legs tarsus thinner

elytra potentially blistered

elytra shape rounded

labial palp potentially not fused

labial palp elongated

labial palp position irregular

labial palp shape thinner

maxillary palp shape thinner

female genital lobes slightly rounded

wings shortened

maxillary palp elongated

pupal molt delayed (still larva)

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number hatched none (< 10 %)

adult lethality


pupal molt delayed (still larva)

adult lethality


larva lethality

adult lethality

adult lethality

adult lethality


larva lethality

adult lethality


eclosion not fulfilled (died as pupa)

wings partially potentially necrotic

wings shortened

antenna orientation irregular

prothoracic leg shortened

antenna shortened

larva lethality

vitellogenic egg chamber late number increased

larva number hatched none (< 10 %)

egg production: number <50% of expected


pupal molt delayed (still pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

legs irregular

legs shortened

cuticle remnants with some identifiable segments

larva dorsal not closed

head & thorax & abdomen partially not present

thorax & abdomen partially not present

appendages random remaining

cuticle remnants without visible segmentation, differentiation or axis

larval musculature pattern irregular

larva number few (< 50 %) hatched

adult lethality


larva lethality

affected eggs/embryos/larvae

head partially not present

head shape irregular


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

egg chamber number decreased

vitellogenic egg chamber late not present

adult lethality

adult nutritional state potentially bad (cachectic)

egg production: number no/almost no eggs

adult lethality

adult lethality

adult lethality

adult nutritional state bad (cachectic)

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

larva number hatched none (< 10 %)

adult lethality

adult lethality


larva lethality

larva number hatched many (ca. 70-100 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality

affected eggs/embryos/larvae

larval musculature pattern irregular

affected eggs/embryos/larvae

head & thorax partially not present

head & thorax complete not present

head partially not present

All kind of head defects or missing head parts are possible. From just shortened antennae (as shown) to several completely missing head appendages.

head musculature not present

larva number few (< 50 %) hatched

about 40-50%


larva lethality late (L7)

larva lethality early (L5/6)

adult lethality

adult nutritional state bad (cachectic)

adult lethality


pupal molt delayed (still larva)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

mesobasisternum posterior dv-orientation irregular

prosternum anterior shape irregular

prosternum anterior rim irregular

eclosion not fulfilled (died as pupa)

nearly completely eaten up

wings not closed

pupal molt delayed (still larva)

wings shortened

antenna slightly orientation irregular

wings not closed

head slightly orientation irregular

pupal molt delayed (still larva)

affected eggs/embryos/larvae

larva cuticle thinner

larva appendages shortened

larva segmentation/division incomplete

adult lethality


larva lethality

affected eggs/embryos/larvae

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

with embryonic tissue, no cuticle, but muscle fluorescence

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larval musculature irregular

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality

egg production: number <50% of expected

adult lethality


larva lethality late (L7)

adult lethality

adult lethality

adult lethality


adult lethality

ovary without detectable defects

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

adult lethality

adult nutritional state bad (cachectic)

mesothorax coxa protruding

intercoxal process T3 not closed

intercoxal process T3 potentially shortened

motility decreased

all animals cannot walk or turn themselves; joint between femur and tibia cannot be mved by the animal although the joint itself is functional and movable by external manipulation

mesobasisternum posterior irregular

appendages sometimes remaining

larval musculature irregular

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched

adult lethality


pupal molt delayed (still larva)

affected eggs/embryos/larvae

gena triplet seta number increased

head bristle pattern irregular

legs slightly shape irregular

head potentially size decreased

All head appendages are present. Their shape looks irregular in a few cases. Especially when looking at the distance between the median and posterior vertex bristles the head size seems to be reduced.

larval musculature partially not present

larval musculature irregular

larva number few (< 50 %) hatched

adult lethality


previtellogenic egg chamber potentially number decreased

somatic plug may be reduced or is at least not visible

egg chamber not present

tropharium shortened

egg production: number <50% of expected

adult appendages distal colour decreased

pupal molt

pupal molt not completely molted

affected eggs/embryos/larvae

larva dorsal not closed

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larval musculature irregular

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

vitellogenic egg chamber late number decreased

vitellogenic egg chamber late number increased

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

About 50%

egg production: number <50% of expected

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


eclosion delayed (still larva)

ovary without detectable defects

egg production: number <50% of expected

egg production: number <50% of expected


larva lethality

adult lethality

pupa eclosion not fulfilled (died as pupa)


pupal molt not fulfilled (died as prepupa)

head appendages shortened

abdomen segment number decreased

legs podomer potentially fused

head partially not present

The head often appears smaller, in some cases also complete parts (appendages etc.) or almost the whole head are missing.

larva appendages shortened

larval musculature fluorescence decreased

embryo/egg not developed/not fertilized

larva number hatched many (ca. 70-100 %)

eclosion not fulfilled (with pupal and adult features)


larva lethality

larval musculature pattern irregular

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

larva number few (< 50 %) hatched

egg production: number <50% of expected

About 50%

adult lethality


ovary without detectable defects

mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

affected eggs/embryos/larvae

legs podomer partially fused

legs shortened

cuticle remnants without visible segmentation, differentiation or axis

legs shape irregular

head & thorax & abdomen partially not present

legs sometimes remaining

cuticle remnants with some identifiable segments

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality

egg production: number <50% of expected

adult lethality


larva lethality

adult lethality


larva lethality late (L7)

larva lethality early (L5/6)

adult lethality

adult nutritional state bad (cachectic)

adult lethality

adult lethality


adult lethality

elytra surface irregular

hindwing folding irregular

elytra blistered

wings not closed

eclosion not completely molted

oblique dorsal thoracic muscles median slightly shortened

oblique dorsal thoracic muscles median broader

elytra blistered

antenna orientation irregular

head & thorax partially not present

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larval musculature irregular

embryo/egg not developed/not fertilized

larva number few (< 50 %) hatched

adult lethality


fertility after larval injection decreased (no living offspring)

larva lethality

affected eggs/embryos/larvae

affected eggs/embryos/larvae

cuticle remnants with unidentifyable segments

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality

adult lethality


ovary without detectable defects

egg production: number <50% of expected


eclosion not fulfilled (died as pupa)

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

pupa eclosion not fulfilled (died as pupa)

adult lethality


eclosion delayed (still pupa)

eclosion delayed (still pupa)

adult lethality


larva lethality late (L7)

affected eggs/embryos/larvae

legs podomer partially shortened

egg production: number <50% of expected

adult lethality

adult lethality


larva lethality late (L7)

larva lethality early (L5/6)

adult lethality

adult lethality


adult lethality

adult lethality

adult lethality


eclosion not fulfilled (died as pupa)

hindwing partially blistered

longitudinal dorsal thoracic muscles partially not present

elytra blistered

larva lethality

vitellogenic egg chamber partially fused

vitellogenic egg chamber late number decreased

vitellogenic egg chamber late partially rounded

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


thorax & abdomen dorsal not closed

larva appendages shortened

larval musculature potentially degenerated

larval musculature partially not present

vitellogenic egg chamber late number increased

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality early (L5/6)

tropharium structure irregular

vitellogenic egg chamber late degenerated

mature egg not present

adult lethality

egg production: number no/almost no eggs

adult lethality


eclosion not fulfilled (died as pupa)

adult lethality

hindwing folding irregular

elytra not closed

elytra shortened

elytra surface irregular

postcoxal bridge shortened

prosternum differentiation irregular

the prosternum has openings/slits at the coxae pointing antero-lateral

prothorax anterior rim rounded

eclosion not fulfilled (not completely eclosed)

prothorax size decreased

adult slightly size decreased

pronotum slightly shape irregular

gin-traps shape irregular

legs slightly orientation irregular

wings not closed

antenna orientation irregular

abdomen gin-traps size decreased

wings shortened

head slightly orientation irregular

pupal molt delayed (still larva)

larva lethality

affected eggs/embryos/larvae

head seta partially not present

head & thorax bristle dorsal partially not present

thorax partially potentially not closed

Looks like an invagination of dorsal cuticle.

vertex triplet seta partially not present

head & thorax & abdomen bristle pattern irregular

larval musculature fluorescence decreased

larval musculature fluorescence degenerated

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number hatched none (< 10 %)

adult lethality


larva lethality

affected eggs/embryos/larvae

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

with embryonic tissue, no cuticle, no fluorescence

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


adult lethality

elytra surface irregular

wings dorsal not closed

gula shape irregular

boarders lowered in, surface folded

adult lethality

affected eggs/embryos/larvae

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

ovary need term without detectable defects

vitellogenic egg chamber late partially number increased

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

pupa eclosion not fulfilled (died as pupa)


larva lethality

affected eggs/embryos/larvae

larval musculature irregular

larva number hatched many (ca. 70-100 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

ovary without detectable defects

egg production: number <50% of expected

adult lethality


fertility after larval injection decreased (no living offspring)

adult lethality

previtellogenic egg chamber potentially number decreased

tropharium posterior potentially size decreased

the number of proocytes and the somatic plug seems to be reduced

tropharium structure irregular

tropharium shape irregular

tropharium size decreased

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number no/almost no eggs

adult bristle pattern irregular

the depressions of the sensory bristles seem to be enlarged (diameter increased, maybe deeper)

affected eggs/embryos/larvae

larval musculature partially not present

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larval musculature pattern irregular

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult nutritional state bad (cachectic)

adult lethality

adult lethality


larva lethality

affected eggs/embryos/larvae

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


pupal molt delayed (still larva)


larva lethality

adult lethality

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality early (L5/6)

abdomen segment number decreased

abdomen potentially transformed

abdomen partially not present

legs number increased

abdomen segment number decreased

thorax segment number decreased

head appendages random not present

cuticle remnants with some identifiable segments

adult lethality


pupal molt not fulfilled (died as prepupa)

larva lethality

tropharium sometimes irregular

vitellogenic egg chamber sometimes degenerated

previtellogenic egg chamber decreased

vitellogenic egg chamber late decreased


larva lethality late (L7)

larva lethality early (L5/6)

pupal molt delayed (still larva)

larva lethality early (L5/6)

adult lethality

adult lethality

adult lethality


larva lethality

adult lethality


affected eggs/embryos/larvae

affected eggs/embryos/larvae

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (died as pupa)


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality

adult lethality


mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

ovary without detectable defects

egg production: number <50% of expected

with embryonic tissue, no cuticle, no fluorescence

abdomen bristle ventral pattern irregular

thorax & abdomen segment number decreased

cuticle remnants strongly fragmented (cuticle crumbs)

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)

pupa eclosion not fulfilled (died as pupa)


larva lethality

3 died during a larval molt

adult lethality

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

vitellogenic egg chamber shape irregular

vitellogenic egg chamber mostly number decreased

previtellogenic egg chamber complete not present

egg production: number no/almost no eggs

adult lethality


fertility after larval injection decreased (no living offspring)

adult lethality

eclosion delayed (still pupa)

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)


larva lethality

larva number hatched many (ca. 70-100 %)


ovary without detectable defects

tropharium slightly shape irregular

vitellogenic egg chamber slightly number decreased

egg production: number <50% of expected

hindwing shape irregular

elytra partially blistered

gula surface irregular

eclosion not fulfilled (died as pupa)

elytra proximal partially blistered

larva lethality

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality early (L5/6)

egg chamber mostly not present

egg chamber not present


larva lethality

affected eggs/embryos/larvae

affected eggs/embryos/larvae

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


eclosion delayed (still pupa)

pupal molt delayed (still larva)


elytra partially colour lighter

pupal molt delayed (still larva)

larva lethality


pronotum seta shortened

larva lethality

adult lethality


legs orientation irregular

prothoracic leg shortened

legs potentially shortened

wings orientation irregular

wings shortened

head slightly shape irregular

wings orientation irregular

legs orientation irregular

legs potentially shortened

wings shortened

head slightly shape irregular

pupal molt delayed (still larva)

larva lethality

affected eggs/embryos/larvae

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

ovary without detectable defects

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


abdominal odoriferous glands slightly colour darker

waited 1 week longer to let the phenotype develop

adult lethality

eclosion delayed (still pupa)

adult lethality


vitellogenic egg chamber sometimes differentiation irregular

some of the egg chambers seem polynucleated

ovariole sometimes duplicated

previtellogenic egg chamber potentially position irregular

egg chamber number decreased

tropharium size decreased

egg production: number <50% of expected


larva lethality

adult lethality

adult lethality

adult lethality


larva lethality


pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still prepupa)

tropharium potentially irregular

vitellogenic egg chamber potentially degenerated

vitellogenic egg chamber number decreased

eclosion not fulfilled (with pupal and adult features)


larva lethality

affected eggs/embryos/larvae

adult lethality


adult lethality immediately after eclosion

elytra blistered

affected eggs/embryos/larvae

larval musculature fluorescence decreased

embryo/egg with embryonic tissue, no cuticle, no fluorescence

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larva number few (< 50 %) hatched


larva lethality

head & thorax partially present

cuticle remnants with unidentifyable segments

larva number few (< 50 %) hatched

adult lethality

pupa eclosion not fulfilled (died as pupa)


fertility after larval injection decreased (no living offspring)

eclosion delayed (still pupa)

head slightly orientation irregular

pupal molt delayed (still larva)

abdomen everted (inside-out)

larva appendages shape irregular

head & thorax & abdomen everted (inside-out)

larva number hatched none (< 10 %)

adult lethality

adult lethality


eclosion delayed (still pupa)

larva lethality

affected eggs/embryos/larvae

affected eggs/embryos/larvae

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larva number hatched none (< 10 %)


egg chamber mostly not present

tropharium size decreased

ovariole sometimes not present

ovary size decreased

vitellogenic egg chamber late sometimes number increased

egg production: number no/almost no eggs

larva number hatched many (ca. 70-100 %)


adult lethality

larva lethality late (L7)

pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality


not fulfilled (died as prepupa)

larva lethality

affected eggs/embryos/larvae

affected eggs/embryos/larvae

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

larva number hatched none (< 10 %)

adult lethality

egg production: number <50% of expected


larva lethality

affected eggs/embryos/larvae

head & thorax decreased

head decreased

head & thorax complete not present

abdomen posterior remaining

larva number hatched none (< 10 %)

adult lethality


mature egg deposition 2 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

ovary without detectable defects

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

wings not closed

eclosion delayed (still pupa)

wings shortened

antenna slightly orientation irregular

pupal molt delayed (still larva)

larva lethality

adult lethality

eclosion not fulfilled (with pupal and adult features)


posterior terminus shape irregular

Same phenotype present in hatched animals.

posterior terminus complete not present

abdomen segment number decreased

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

vitellogenic egg chamber number decreased

previtellogenic egg chamber complete not present

ovary size decreased

adult lethality

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

pupal molt not fulfilled (died as prepupa)

larva lethality

pupal molt delayed (still larva)

pupal molt delayed (still larva)

egg chamber complete not present

adult lethality


pupal molt delayed (still larva)

larva lethality

affected eggs/embryos/larvae

abdomen shape irregular

thorax & abdomen segment number decreased

cuticle remnants with unidentifyable segments

larva number few (< 50 %) hatched

adult lethality

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

egg chamber mostly not present

ovary size decreased

adult lethality

adult lethality

larval musculature pattern irregular

affected eggs/embryos/larvae

adult lethality


larva lethality early (L5/6)

larva lethality late (L7)

adult lethality

adult lethality

adult lethality


fertility after larval injection decreased (no living offspring)

affected eggs/embryos/larvae

affected eggs/embryos/larvae

cuticle remnants without visible segmentation but polarity

cuticle everted (inside-out)

cuticle remnants strongly fragmented (cuticle crumbs)

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

GFP-positive tube-like structure present 30-50% (potentially gut?)

larva number hatched none (< 10 %)


larva lethality

affected eggs/embryos/larvae

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality

adult lethality


ovary without detectable defects

egg production: number <50% of expected

eclosion not fulfilled (died as pupa)

eclosion not fulfilled (not completely eclosed)

elytra partially blistered

elytra partially blistered

larva number hatched none (< 10 %)

adult lethality


larva lethality

larva number hatched many (ca. 70-100 %)


vitellogenic egg chamber shape rounded

tropharium size decreased

egg production: number no/almost no eggs

larva lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality

pupa eclosion not fulfilled (died as pupa)


larva lethality late (L7)

egg chamber complete not present

adult lethality

adult lethality


adult lethality

ovary without detectable defects

egg production: number <50% of expected

hindwing folding irregular

wings slightly shortened

metathoracic leg slightly shortened

elytra shape irregular

elytra surface irregular

gula shape irregular

prothorax anterior rim bent

head shape irregular

pregnathal and gnathal head is somehow bent to the ventral side in relation to the postgnathal head

wings slightly orientation irregular

head size decreased

larva lethality

affected eggs/embryos/larvae

abdomen cuticle number decreased

head appendages sometimes not present

larva number hatched none (< 10 %)

adult lethality


abdominal odoriferous glands colour decreased

ovaries were extremely held-egg (>10 eggs in one lateral oviduct)

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

pronotum bristle dorsal median surface not present

dorsal medium line without bristles

wings not closed

pupa seta partially shortened

affected eggs/embryos/larvae

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)


larva lethality

egg production: number <50% of expected

adult lethality


larva lethality late (L7)

eclosion not fulfilled (died as pupa)

pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality

adult lethality


larva lethality late (L7)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality

adult lethality


pupal molt delayed (still larva)

pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still larva)

pupal molt not fulfilled (died with pupal and larval features)

all dead animals partially kannibalized

pupal molt not fulfilled (died as pupa)

larva lethality

tropharium potentially size decreased

vitellogenic egg chamber late not present

ovary size decreased

vitellogenic egg chamber number decreased

previtellogenic egg chamber complete not present

adult lethality

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality

adult lethality


larva lethality

adult lethality


pupal molt delayed (still prepupa)

larva lethality late (L7)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

larva lethality

adult lethality

adult nutritional state bad (cachectic)

adult lethality


larva lethality

adult lethality

adult lethality


adult lethality

larva lethality

adult lethality

adult nutritional state bad (cachectic)

egg production: number <50% of expected

egg production: number <50% of expected


eclosion not fulfilled (died as pupa)

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality


ovariole mostly not present

tropharium shape irregular

tropharium size decreased

tropharium structure irregular

cells of tropharia appear bigger than usual

vitellarium mostly not present

egg chamber not present

adult lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

egg production: number <50% of expected


adult lethality

adult lethality

elytra shortened

elytra not closed

adult lethality

wings shortened

larva lethality

cuticle remnants without visible segmentation, differentiation or axis

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg not developed/not fertilized

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

larva number hatched none (< 10 %)


fertility after larval injection decreased (no living offspring)

adult lethality

adult lethality

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

wings bent

wings shortened

pupal molt delayed (still prepupa)

gut mostly remaining

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number hatched none (< 10 %)

adult lethality


abdominal odoriferous glands sometimes darker

adult lethality

oblique dorsal thoracic muscles median not present

larva number hatched many (ca. 70-100 %)

adult lethality


larva lethality

abdomen musculature random partially not present

adult lethality


adult lethality

adult lethality

pupa eclosion not fulfilled (died as pupa)


pupal molt delayed (still prepupa)

pupal molt delayed (still prepupa)

larva lethality late (L7)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

larva lethality

vitellarium complete thinner

vitellogenic egg chamber complete not present

previtellogenic egg chamber partially number decreased

tropharium partially structure irregular

egg production: number no/almost no eggs

eclosion not fulfilled (with pupal and adult features)


pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

larva lethality

mature egg deposition irregular

egg chamber number decreased

mature egg number increased

mature egg posterior colour darker

mature egg posterior protruding

egg production: number <50% of expected

adult lethality


previtellogenic egg chamber number decreased

mature egg not present

vitellarium shortened

larva lethality

vitellarium size decreased

previtellogenic egg chamber position irregular

vitellogenic egg chamber not present

larva number hatched none (< 10 %)

egg production: number <50% of expected


adult lethality

adult lethality

larva lethality

larva everted (inside-out)

abdomen segmentation/division irregular

head & thorax partially not present

abdomen partially remaining

cuticle remnants without visible segmentation, differentiation or axis

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larval musculature pattern irregular

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

adult lethality


eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality

adult lethality


pupal molt not fulfilled (died as prepupa)

pupal molt delayed (still prepupa)

larva lethality

adult lethality

adult lethality


larva lethality

larval musculature irregular

larva number few (< 50 %) hatched


adult lethality

larva lethality

larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

larva lethality

adult lethality


adult lethality

adult lethality

eclosion not fulfilled (died as pupa)

adult lethality

elytra dorsal not closed

eclosion delayed (still pupa)

larva lethality

ovary without detectable defects

mature egg deposition 3 4 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

egg production: number <50% of expected


pupal molt not fulfilled (died with pupal and larval features)

pupal molt delayed (still prepupa)

larva lethality

adult lethality


larva lethality

abdomen partially dorsoventral-axis broader

adult lethality

embryo/egg with embryonic tissue, no cuticle, no fluorescence

abdomen segmentation/division irregular

larval musculature pattern irregular

larva number few (< 50 %) hatched


adult lethality

elytra shape irregular

elytra not closed

adult lethality

larva bristle mostly not present


adult lethality

adult lethality

motility decreased

adult lethality

elytra dorsal not closed

eclosion delayed (still pupa)

wings shortened

larva lethality


fertility after larval injection decreased (no living offspring)

larva lethality

abdomen dorsal not fused

larva bristle mostly number decreased

legs segment mostly fused

seems that trochanter & coxa are missing

larva dorsal bent

larva appendages shortened

larva size decreased

abdomen ectopically segmentation/division irregular

whole abdomen appears crumpled-up -> no segments identifyable

head capsule shape irregular

larva everted (inside-out)

larval musculature pattern irregular


eclosion not fulfilled (died as pupa)

adult lethality

eclosion not fulfilled (died as pupa)

elytra dorsal not closed

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality

embryo/egg not developed/not fertilized

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality

adult lethality


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality

legs pretarsus shape irregular

head appendages random shape irregular

abdomen central dorsoventral-axis increased

legs tibiotarsus shape irregular

head capsule size decreased

larva appendages shape irregular

leg musculature tb1-ptar1 random not present

leg musculature femural musculature random not present

50-80 % of the larvae seemed to have no muscles in the femur & tibiotarsus, randomly in 1 up to all 6 legs.

larva number few (< 50 %) hatched

adult lethality


adult lethality

adult lethality

larva lethality

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

eclosion not fulfilled (with pupal and adult features)

adult lethality


adult lethality

adult lethality


larva lethality

adult lethality

egg production: number no/almost no eggs

adult lethality

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult nutritional state

adult lethality


adult lethality

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)


eclosion not fulfilled (died as pupa)

longitudinal dorsal thoracic muscles sometimes thinner

oblique dorsal thoracic muscles decreased

oblique dorsal thoracic muscles thinner

wings bent

antenna orientation irregular

pupal molt delayed (still prepupa)

larva lethality

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg not developed/not fertilized

ovary without detectable defects

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality

pupa eclosion not fulfilled (died as pupa)


adult lethality

larva lethality

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


larva lethality

larva number hatched many (ca. 70-100 %)


pupal molt not fulfilled (died as prepupa)

pupal molt not fulfilled (died as L7 or prepupa)

pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

larva lethality

adult nutritional state bad (cachectic)


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality

larval musculature irregular

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality


adult lethality

larva lethality

adult lethality


eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

eclosion not fulfilled (died as pupa)

longitudinal dorsal thoracic muscles not present

pupal molt delayed (still larva)

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

adult lethality


eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

adult lethality

head & thorax & abdomen partially not present

legs segment shortened

procephalic appendages mostly not present

sometimes one deformed antenna remaining

head & thorax partially not present

larval musculature irregular

larva number hatched none (< 10 %)

adult lethality immediately after eclosion


eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

adult lethality


pupal molt not fulfilled (died with pupal and larval features)

pupal molt delayed (still prepupa)

larva lethality

adult lethality


adult lethality

elytra not closed

adult lethality

larva lethality

legs random sometimes shape irregular

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number few (< 50 %) hatched

eclosion not fulfilled (with pupal and adult features)


larva lethality

egg production: number <50% of expected

adult lethality


eclosion not fulfilled (died as pupa)

legs sometimes not present

wings size decreased

antenna orientation irregular

pupal molt delayed (still prepupa)

larva lethality

larva number hatched none (< 10 %)

adult lethality


adult lethality

vitellarium sometimes shape irregular

egg chamber number decreased

vitellarium sometimes shortened

tropharium size decreased

eclosion delayed (still pupa)

mature egg not present

egg production: number no/almost no eggs

pupal molt delayed (still prepupa)

pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

larva lethality

head mostly remaining

Cuticle ball with antennae, labrum, residues of maxillae und mandibles.No labium.


adult lethality

eclosion not fulfilled (died as pupa)

elytra posterior necrotic

pupal molt delayed (still larva)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

gena triplet pattern irregular

head appendages position irregular

abdomen complete anterior constricted

labrum shape irregular

larval musculature pattern irregular

ovary without detectable defects

larva number hatched none (< 10 %)

adult lethality


larva lethality

adult lethality


pupal molt delayed (still larva)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality

adult lethality


pupal molt delayed (still larva)

ovary without detectable defects


adult lethality

eclosion delayed (still pupa)

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still prepupa)

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality

abdominal odoriferous glands mostly colour darker

adult nutritional state bad (cachectic)

egg production: number <50% of expected


pupal molt delayed (still larva)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still larva)

pupal molt delayed (still larva)

eclosion delayed (still pupa)

larva lethality late (L7)

wings bent

pupal molt delayed (still larva)

larva lethality

adult lethality

adult lethality


fertility after larval injection decreased (no living offspring)

egg chamber mostly not present

tropharium shortened

mature egg not present

egg production: number <50% of expected

vitellarium mostly decreased

larva lethality

ovary without detectable defects

mature egg deposition 5 >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected


larva lethality

adult lethality

adult lethality

adult lethality


pupal molt delayed (still prepupa)

pupal molt delayed (still larva)

larva lethality

mature egg deposition 3 >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larval musculature irregular

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality


pupal molt delayed (still prepupa)

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

larva lethality late (L7)

pupal molt delayed (still larva)

adult lethality

eclosion delayed (still pupa)

pupal molt delayed (still larva)

pupal molt not fulfilled (died as pupa)

pupal molt delayed (still larva)

pupal molt delayed (still prepupa)

larva lethality

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


pupal molt not fulfilled (died as prepupa)

wings bent

pupal molt delayed (still prepupa)

larva lethality

previtellogenic egg chamber sometimes deposition irregular

Early oocytes are not lined up, but appear accumulated right below the tropharium.

previtellogenic egg chamber number decreased

vitellogenic egg chamber mostly not present

vitellarium shortened

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


adult lethality

ovary without detectable defects

adult lethality

larva lethality

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality

pupa eclosion not fulfilled (died as pupa)


adult lethality

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva number hatched many (ca. 70-100 %)


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality

adult lethality


adult lethality

larva lethality

adult lethality


adult lethality

tropharium shortened

mature egg not present

egg chamber number decreased

tropharium shape irregular

egg production: number <50% of expected


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

adult nutritional state bad (cachectic)

larva number hatched none (< 10 %)

egg production: number <50% of expected

adult lethality


adult lethality

eclosion delayed (still pupa)

larva lethality

embryo/egg not developed/not fertilized

embryo/egg with embryonic tissue, no cuticle, no fluorescence

larva number hatched none (< 10 %)


adult lethality

pupal molt delayed (still prepupa)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still prepupa)

eclosion not fulfilled (died as pupa)

pupal molt delayed (still prepupa)

oblique dorsal thoracic muscles thinner

pupal molt delayed (still larva)

longitudinal dorsal thoracic muscles mostly not present

sometimes attachment point visible, but never 3 on one side

wings blistered

antenna orientation irregular

pupal molt delayed (still prepupa)

adult nutritional state bad (cachectic)

adult lethality


larva lethality

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality

larval musculature pattern irregular


eclosion not fulfilled (died as pupa)

wings blistered

wings orientation irregular

hindwing blistered

pupal molt not fulfilled (died as pupa)

larva lethality

gnathal appendages shape irregular

head capsule size decreased

head appendages shortened

larval musculature partially rounded

larval musculature partially orientation irregular

larval musculature partially shortened

larval musculature partially not present

mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected


eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

gena triplet seta pattern irregular

thorax bristle pattern irregular

gena triplet seta number increased

Due to the lack of GFP-Signal, status of tissue, eg. presence of muscles, is not scorable.

larval musculature fluorescence not present

Majority of animals still lays in the egg shell. If outside they appear in an u-shape, with the head bent towards the abdomen. All fluorescence is missing, musculature as well as nervous system and eyes.

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality

adult lethality


adult lethality

adult lethality

larva lethality

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

adult lethality

adult lethality

adult lethality


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

adult lethality

pupal molt delayed (still larva)

pupal molt delayed (still larva)


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

adult lethality

pupal molt delayed (still larva)

pupal molt delayed (still larva)

adult lethality

adult lethality


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality


ovary without detectable defects

mature egg deposition 2 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

egg production: number <50% of expected

eclosion not fulfilled (died as pupa)

adult lethality

eclosion not fulfilled (died as pupa)

elytra not closed

eclosion delayed (still pupa)

pupal molt delayed (still prepupa)

larva lethality

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)

adult lethality

adult lethality immediately after eclosion

larval musculature irregular


pupal molt delayed (still prepupa)

pupal molt not fulfilled (died as L7 or prepupa)

labrum elongated

maxilla size increased

antenna elongated

head capsule slightly size decreased

Bristle pattern possible impaired

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality)

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

legs pretarsus rounded

legs pretarsus shortened

cuticle remnants with unidentifyable segments

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

leg musculature sometimes rounded

leg musculature not present

larva number few (< 50 %) hatched


pupal molt delayed (still larva)

pupal molt delayed (still larva)

larva lethality

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

adult lethality

adult lethality


larva lethality


adult lethality

adult lethality

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

larva lethality

adult lethality


eclosion not fulfilled (died as pupa)

adult lethality

eclosion not fulfilled (not completely eclosed)

eclosion delayed (still pupa)

elytra orientation irregular

mature egg deposition 3 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

larva number hatched none (< 10 %)

egg production: number <50% of expected


eclosion delayed (still pupa)

larva lethality

prothoracic leg pretarsus not present

prothoracic leg shape irregular

mesothorax not present

abdomen not present

metathorax not present

antenna mostly not present

appendages partially remaining

cuticle remnants with some identifiable segments

embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence

larval musculature irregular

larva number few (< 50 %) hatched

adult lethality


larva lethality

adult lethality

larva number hatched many (ca. 70-100 %)

adult lethality


adult lethality

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

larva lethality

larva bent


adult lethality

adult lethality

eclosion not fulfilled (died as pupa)

dorsal thoracic muscles sometimes structure irregular

larva lethality


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

adult lethality


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult nutritional state potentially bad (cachectic)

larva number hatched none (< 10 %)

egg production: number <50% of expected


eclosion not fulfilled (died as pupa)

larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected


eclosion not fulfilled (died as pupa)

larva lethality

larval musculature irregular


eclosion not fulfilled (died as pupa)

larva lethality


adult lethality

oblique dorsal thoracic muscles

larva lethality

larval musculature irregular

embryo/egg with embryonic tissue, no cuticle, no fluorescence


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

larva cuticle duplicated

leg musculature sometimes not present

larval musculature partially random not present

abdomen musculature ventral partially not present

larval musculature dorsal partially not present

effect clearly stronger in the abdominal musculature

larva number few (< 50 %) hatched

adult lethality

eclosion not fulfilled (with pupal and adult features)


adult lethality

adult lethality immediately after eclosion

oblique dorsal thoracic muscles partially shortened

longitudinal dorsal thoracic muscles number irregular

elytra ventral not closed

larva lethality

larva cuticle dorsal irregular

abdomen segment potentially size decreased

legs segment shortened

head & thorax & abdomen partially not present

appendages partially remaining

egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide

larval musculature irregular

larva number hatched none (< 10 %)


mature egg deposition >5 blocked number 1st lateral oviduct: number 2nd lateral oviduct:

adult lethality

larva lethality

head & thorax cuticle dorsal irregular

abdomen segment number decreased

legs segment sometimes shortened

head capsule partially not present

appendages partially remaining

cuticle remnants with some identifiable segments

larval musculature irregular

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

eclosion not fulfilled (died as pupa)

head & thorax partially not present

appendages partially remaining

larval musculature irregular

larva number few (< 50 %) hatched


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

legs number increased

antenna shape irregular

abdomen segment number decreased

antenna not present

larval musculature irregular

larva number few (< 50 %) hatched


adult lethality

eclosion not fulfilled (died as pupa)

larva lethality

embryo/egg not developed/not fertilized

vitellarium shortened

egg chamber number decreased

adult lethality

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected

adult lethality


pupal molt not fulfilled (died as prepupa)

larva lethality

vitellogenic egg chamber number decreased

previtellogenic egg chamber not present

larva number hatched none (< 10 %)

egg production: number no/almost no eggs

egg production: number <50% of expected

adult lethality


adult lethality

larva lethality

larva number hatched many (ca. 70-100 %)

adult lethality


eclosion not fulfilled (died as pupa)

larva lethality

fertility after larval injection decreased (no living offspring)

adult lethality

eclosion not fulfilled (not completely eclosed)

larva lethality

legs segment slightly shortened

larva number few (< 50 %) hatched

adult lethality

embryo/egg without visible larval cuticle in cuticle preparation

affected eggs/embryos/larvae

embryo/egg not developed/not fertilized

affected eggs/embryos/larvae

larva number hatched none (< 10 %)

adult lethality

eclosion not fulfilled (with pupal and adult features)

eclosion not fulfilled (died as pupa)


adult lethality

eclosion not fulfilled (died as pupa)

elytra need term size elongated

elytra dorsal not closed

antenna orientation bent

larva lethality

ovary without detectable defects

larva number few (< 50 %) hatched

egg production: number <50% of expected

egg production: number <50% of expected


egg production: number >150% of expected

No phenotype!

eclosion not fulfilled (died as pupa)

larva lethality

larva number few (< 50 %) hatched

adult lethality


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

eclosion not fulfilled (died as pupa)

larva lethality

larval musculature irregular

larva number few (< 50 %) hatched


adult lethality immediately after eclosion

eclosion not fulfilled (died as pupa)

larva lethality

mesothorax not present

abdomen complete not present

maxilla fragmented

mandible remaining

labrum remaining

abdomen segment number decreased

metathorax not present

antenna remaining

larval musculature irregular

larva number few (< 50 %) hatched

adult lethality


larva lethality

larva number few (< 50 %) hatched


adult lethality

vitellogenic egg chamber not present

Probably compound egg chambers!

eclosion not fulfilled (died as pupa)

larva lethality

egg chamber complete not present

larva number hatched none (< 10 %)


larva lethality late (L7)

pupal molt delayed (still larva)

larva lethality

adult lethality

larva number hatched none (< 10 %)

adult lethality

egg production: number <50% of expected

adult lethality


adult lethality

not fulfilled (died as pupa)

larva lethality

antenna not present

maxilla not present

labrum not present

head appendages shape irregular

abdomen posterior constricted

abdomen segment number decreased

head size decreased

appendages partially remaining

larval musculature irregular

larva number few (< 50 %) hatched


eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

eclosion not fulfilled (died as pupa)

gena size decreased

legs tibia structure irregular

legs femur structure irregular

Femur and tibia were thicker/fatter than usual.

antenna orientation bent

labial palp structure irregular

In some cases, the labial palps were completely swollen and maxillary palps seemed to be transformed into antennae!

elytra shortened

head appendages pattern broader

The distance between the labrum/mandible to the labial and maxillary palps increased!

larva lethality

head capsule size decreased

legs tibiotarsus shortened

posterior terminus shape irregular

legs femur shortened

head appendages shape irregular

leg musculature tb1-ptar1 shortened

Muscle defect due to defects in the leg morphology (tibiotarsus looks much sorter than normal)

larva number few (< 50 %) hatched


eclosion not fulfilled (died as pupa)

adult lethality

eclosion delayed (still pupa)

larva lethality

embryo/egg with embryonic tissue, no cuticle, no fluorescence

ovary without detectable defects

larva number hatched none (< 10 %)

egg production: number <50% of expected

egg production: number <50% of expected


adult lethality

eclosion not fulfilled (died as pupa)

eclosion delayed (still pupa)

larva lethality

labrum potentially not present

head dorsal protruding

antenna not present

in many cases only one antenna missing

head capsule mostly not present

larva number few (< 50 %) hatched


egg production: number <50% of expected

No phenotype present!

adult lethality

eclosion delayed (still pupa)

larva lethality

embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence

embryo/egg not developed/not fertilized

larva number hatched none (< 10 %)