Choose the stage and click on a term on the list of morphological structures, which will appear upon typing into the respective field.
Optional: Choosing a penetrance of >50% will strongly reduce false positive hits.

Search strategy

The search at iBeetle-Base allows for very specific searches (e.g. “larva, leg, size decreased”). However, in order not to miss interesting phenotypes, we recommend initiating your search broadly for defects of the structure and stage you are interested in (larva, leg). Further, a penetrance of >50% is recommended in order to increase the portion of reproducible datasets. Next, the list should be consolidated by browsing the pictures and detailed annotations displayed in the search results. Interesting results can be marked by checkboxes. Finally, the respective details pages should be browsed in order to learn more about additional phenotypes, the gene structure and sequences and its orthologs.

Reproducibility

Highly penetrant phenotypes with a direct phenotype-genotype relationship (e.g. lethality, wing blistering) were reproducible at a close to 100% rate in our hands. Regarding processes where the relation between gene function and phenotype is complex (e.g. embryonic development), the rate of reproducibility was between 70% and 80%. Reproducibility of low penetrance phenotypes (<50%) was significantly lower.