| General information | |||
| TC009548 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 2266087..2313982 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC009548 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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|---|---|---|
| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
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* : mandatory fields
For help on how to submit larger datasets or non experimental data please contact us.
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_11057 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Analysis of embryonic musculature and early development
number of eggs/embryos/larvae on slide: > 50
larval musculature random pattern irregular -
Penetrance (percentage of animals/eggs on slide): 30% - 50%
larval musculature random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
leg musculature potentially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
larval musculature random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
leg musculature potentially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: > 50
larva dorsal partiallypattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
larva shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
legs tibiotarsus size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
legs tibiotarsus distal shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
legs pretarsus shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
legs pretarsus proximal size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
larva dorsal partiallypattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
larva shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
legs tibiotarsus size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
legs tibiotarsus distal shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
legs pretarsus shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
legs pretarsus proximal size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC009548 RA
MRCDFLLGL LMMVWGAQG GTTTAPATK TASTCDSDQ FQCLDGPCI PSHWRCDGQ PDCADGSDE PFECIQTQT CRPEQFQCA LTRKCLPLG WVCDEDQDC GTSTTLGVD TSDEDPSQC HKEIKCPWN QAKCGFGPE CAPIKKFCD KQRDCVNGG DEWDFCRNS TFSCDKLVC SYGCRPTGE GPKCFCPEG QRPEGSRCV DADECELDN TCAQVCKNT VGSFECSCV SGYEKNGTD CVALNVPPA LDASIVFST QAEIARLKL SGEAWSSNS SLQLLNSTA LEFVFNNHS ICYVHHNFS KASLVCVNI DDFSQKTEL KTQSTLLEF ESVQQMAMD WVSRNWYFL DDQREIFLV CSERLEWCN ILIENDLSK PRALALDPT RGYMFFTKW GHSPPMVER CKMDGSERK AVVNHKIVY PYGVTVDYP TQHIYWVDT YLDFVERVD YDGKDRKTI MRGLKVQNL YGISVFQRK LYLSSWYSN DIIELDKFT MKEKILVQN ISRPFNVLV YHRQRQPVV PHPCNSHSD CSHLCISNW KRDIAVKKC LCAAGYQMT ENNQCVVKP SQRYLLLAK GKPASIKGI ELDSNKETM IPITGINQP MAIDYDVKT GTIIYSDSH RKVIEGAKI NDSSHKTIF QKNVRRCDG LAFDWMSRN VYWTDEEMG SIGVFKLAN SSQNRILLQ DAFYNPLSI VLDPGRGVM YWADWSSIY PNKGRIHVV NMDGKNLKD FVEVNIDWP SGLTIDFVE KRLYWSDRH LRKIESVDF NGVNRRVEL AKVMETPFG LALGPGKTL YFTEFTKGT VMAYNNKTG FRQLDQSNP PIFDIKLYD ATAQQGENE CTKNTPDCP ELCLPTPSD PVCECSSGY KNVNKTCVR DVAPNQAFS VCGPHDFQC KNLKCIPYQ QTCDRIDNC GDQSDEALG PDGPCKDVI CPANQIKCD NQTCISKYW ACDGEQDCV DGSDEDEKR CSEVCSAAQ FKCAVSKRC IPSVWKCDN VADCGPEDM SDEADCVKK QCEVNEFTC ANGRCISQV LYCDGVDDC KDSSDEINC TECQVTEFF CPSTATCLP NSKKCDGQI DCNGGYDEY ECNENLNCG KTEFKCANN LECIPESYV CDGDLDCLD ASDEKHCNK TAHHNTTSP ATSPTCHHP SRFCDNSTK CITVDHLCD NIADCSDGS DEGGRCSEQ LCDLRLDCS HYCHNAPEG LICSCPPDL HLQPDRIHC SKAHPCDTW GVCSQKCIA VGERYKCGC FKGYVLESD RFTCKSTSG ATPYVIFSN RHELKAVEL VTFNVKSFI SSLKNTIAL DFYYTKNAN MVFWTDVID DKIYRGTVV GGSLSNIEV VVQTGLSTA EGLAVDWIG ENLYWVESN LDQIEVAKL DGRFRRTLV AGEMESPRA VAVDPRDGY LFWTDWDNN APRIERCSL AGLDREIVV RVDKIADAG WPNGLTLDY VLRRIYWID ARSDSIHTT KYDGSEHHE VMRNHETLS HPFAIALFE NYVYWTDWR TNSVVRANK FTGGDVAVI QRTLTQPFD IQIMHPARQ PRDGPNPCG VNNGGCSHL CLLHTNRTY RCDCPHVMR LSADNKTCV VNERVLLIA RTSEIRGVD ILQPYYHTI PTISAPQVL NPVQLEYLA RNNTLYWAD SQISEIKRS GLTTGPIQT LIDTGLKNP SGLAIDWLS NLMFVSSPQ GITVSNLDA EFITTIIDN LNVLSVAAH PRLGQLFWI ATTNETFIE TSAMDGSKR TKIVTGLQS HSHSLCVDL HADKLYWVS EFEIYFSNL DGKDVTKLK LPHVSVLAA TVYQEHVYY ADDDDHTIH IVDKMTGGN DTILRNNTG SVFALRIYD PMEQKGFHP CANSQCSDL CLPTSATTF RCRCATGYH TDPQNPNKC VGVEEFIFY SVNWEIRGL ALNGNNQTA VLGPISRIS MATAIDFVA ESDLLFWAD SDRGSITSI KRDGTQRRH IIEPTEAMD SVPVDWLTG LAVDWIAGN MYWCDSKRG VIEVARLDG SKQHVLLAN EIGKPTAIA VDPARGVMV WAGGPRLEI ATMDGRNRN LLVNSSVAI SDVTLDSDQ RKVYFCDTS RHTIEVIGY DGSGHEVLV NDTLENPTA LTILDDKLY WLDKTFEKG SLLSAPLSN LSNYKILLR DVGDFLKDI QIFSKRKQT GTNPCAQNN GGCEQLCLY NGTHPVCVC SHGKISSDG KTCEAFKTF VMYSRVVSI DSIQMLDDE NLQNAPHPS IKNNTLLKN AIGLSFSYK HQRLFYSDI QKGSINAVY YNGTDHRII VEGQGSVEG LAYEQVHNM LYWTCNNHA TINRVNLTD QMTNSSQVE QIVRLRSQD KPRGIAVDS CGARVFWTN WNSHQPSIE RVFLSGYNR EAIIKTDIR MPNALTLDH KAQKLYWGD ARLDKIERC EYDGSNRVI LAKVTPQHP FALAVYADY IYWTDWMLH AVLRADKLT GQNVVWLRR DVARPMGIV TIANDTEDC FYNPCKILN GGCEEICTL NASAMVQCS CMDGRILAE DRKRCYSNT KACDQYDSF RCSDGGCVP FKLTCDGIA HCSDKSDEE PGYCGHRTC LQGWFHCNN KRCVERKDK CNGVDDCGD ASDEENCSC SEDEYFRCS SGECIQKVL RCDNDPDCD DASDEMGCE VRNCTLDFH DGNMINCEN TTACIHKDW FCDGENDCW DWADEKNCT GREKRCDIF QFQCSNGTC ISLEARCDG KDDCHDAKS TRGLSSDEE NCRVMEGHC QNDQFMCGD SRCIPLSWH CNGNPDCLD NSDEYDCHH QCRSDQFKC DNSECIPLS WQCDGHPDC MDQSDESKH CELRECENG DFRCNSTGR CISRLWLCD GEADCLDGA DEHKDQGCG VCTSEHFQC VNGVCINKM YYCDGDKDC NDGSDEPPE CHKTCTSDE FACNNGKCI MDLLKCDGN DDCGDGSDE GKDCHNEGD YCKGKGWFH CGNGVCIND TLLCNGENN CGDFSDETK CRINECTAQ PPPCSQKCV DKPIGYECQ CHTGYQTSA KDKHLCEDI NECLARPCS QLCRNTRGS FHCSCAPGY ILHPDGRTC GSNSIVPVT LILANRYYI REIDMTGQS TLLAHNLTN AVALDYDWS SKCIYWSDV TQLGSSIKR LCNYKNSSS EIEVLHSPT LQNPDGLAV DWVGRNLYW CDKGLDTLE VSSLDGRFR RVLISKGLE EPRAVALDP LRGYLYWTD WGTHAHIGK AGMDGSNQQ IIVNSSLGW PNALTISYE TKELFWADA REDYIAVSD LDGHNIHRI MSREKNHYL QLHHVFAID IWEDYIYWT DWETKSVER CHKYTGNNC TKIMTTVHR PMDIRVVHP LKQPKIKNP CEKANCSAL CLLTPQEPF YKCVCPENY VLKEDGKSC EANCTSAHF ECKKSYKCI PFWWKCDTQ DDCGDGSDE PSDCRPFKC MPGQYQCDN GHCTHPSDL CNGNDDCGD QSDEKDCEH YTCLNTQFR CPGNGTIAP RCIPSKFRC NKHPDCPLG EDESSCPPA TCPPNQFKC ANDKCIPAV WVCDTDNDC GDNSDEQQD CQSRTCSPQ HYRCSSGRC IPMSWRCDG DPDCANNED EPPSCSQPE FHTCEPTYF KCKNNKCIP GRWRCDYDN DCGDSSDEV DCVPRNCSE SEFRCGDGR CIRGAQKCD GEFQCEDRS DEANCHTHC KKNEFQCAN PQVCIYLEW KCDGEADCS DGSDEANCS DTCPDNGFK CHNGLCINE DWRCDGQKD CEDGSDEMF CSLVGCLPG RFRCKNHTC VPVSFLCDG HDQCEDGSD EDPHICHRF NICPPDQFT CKNGHCIKN SLRCDGRND CSDNSDEEN CNNSTCKWN TCSQICIEA KHNVPVCDW ANLVLVSEA ELRLMSPYK PGDSNKLRS KTLATAPGY KVDAVDILY GRTESVAFW TDHQNKRIQ SVVLRLNES RARRDSDVR TVTSGLHDP RGISVDWVA RRLYVTDGV RILASTIDG LYVYTVLRG EMKQPRDIV VVPQDGVLF WSDWGPPAR IETAHMDGN KRRVLVNSA ILYPTGLAV DYTTRRLYW ADPKTMTVE TVKFDGSDR HVVRHFGKE MKPYKIEVF ENYLFVTTY QKHDVLRLN KFGNGNVTH LSQGLTRIA DILILHEQK QDQSVNNTC VDFCHHTEF CLLSPNTAT CTCADGYVK DNLTCKAVA SRTPNCPLN CNSGTCKIV QGQNPKCFC PAQYTGPRC EHFRCSQYC KNKGMCYVD LAAPQSPDS LPPLRCNCA PQWTGERCE TPVNLCDGR CYNGGTCFT PKPEMPVCN CPSGFTGSR CQNCAQLVC QNGGVCVKD TCKCPVGYS GRHCEISFC GKNGKPITT SSGLKCSCL PGFGGEKCE QDRCYQHCQ NGGTCRMGT KQPECDCPA FFGGRRCEI DLCTGGEPP KGCAKRCVC GNGGSCVIM NGRPVCKCQ RPWGGLKCE IYVGNADTC AGLCQNGGV CQILSPHVD PICQCPQSW TGPRCTQLA VCANYCQNG GTCDVSDGL PYCRCPADF DGVKCELLA KLDEAVAHK DVGRGVVVP VIAAIVVII VILVVFLAF DYFYRKRQS FSHERLQEN DFNNPMYQD RDAEPFTLD ADKSGNFAN PVYESVYNG TGSGRGEEK AVLLQHPAD EAPTPAPEE I
- >TC009548 RA
atgaggtgc gattttttg ctgggactg ctgatgatg gtctggggg gcccaaggg ggcaccacc accgctccg gccaccaaa acagcatca acttgcgac agcgaccag ttccaatgc ctggacggc ccttgcatt ccgtcccat tggcgttgc gacggccag ccagactgc gccgacggc agcgacgag cccttcgag tgcatccag acccagacc tgccgtcca gagcaattc caatgtgcc ctcacgcga aagtgccta ccgttgggt tgggtttgc gacgaagac caggactgc gggacctcg acgacgctg ggcgtcgac acctccgac gaggacccc agccagtgc cacaaggag atcaaatgt ccctggaac caggccaag tgcggcttc ggcccagaa tgcgcccca atcaaaaag ttttgtgat aagcagagg gactgtgtt aacggaggc gacgaatgg gatttttgc cggaattcg acgtttagc tgtgataaa ttggtgtgc agctatggg tgcagaccc acaggggag ggacccaag tgcttctgt cccgaggga cagaggccc gaagggagc aggtgtgtg gatgcggac gaatgcgag ctggacaac acttgcgct caagtgtgc aaaaataca gttgggtcg tttgagtgc tcgtgcgtt tcggggtat gagaaaaat gggacggat tgtgtcgca cttaatgtt cctccagcc ctggacgcc tcgatcgtc ttctcaacg caggcggaa atcgcgcgt ttgaagctc tcaggcgaa gcctggtcc tcaaactcc tccctccag ctcctcaac agcacagct ttggagttt gtcttcaac aaccactca atttgctac gtccatcac aacttctcc aaagcgtcg ctagtttgc gtcaacatc gacgatttc tcgcaaaaa accgaacta aaaacccaa tccactttg ctcgaattt gaatccgtc caacaaatg gcaatggat tgggtgtct cgtaattgg tactttttg gacgaccag cgggagatt ttcctggtt tgttccgaa cgtttggag tggtgtaac attttgata gagaacgat ttgagcaag cccagagct ctggctttg gacccgact cgggggtac atgtttttc accaaatgg gggcactcc ccgcccatg gtggagcgg tgtaagatg gacgggagc gagcgcaag gctgtggtg aaccacaag atcgtgtac ccgtatggg gtgactgtt gattatccc acgcagcat atttattgg gttgatacg tatctggat tttgttgag agagttgat tacgacggg aaggacagg aagacgatt atgagggga cttaaggtg caaaatttg tacgggatc agcgttttt caacggaag ttgtatctg tcgtcatgg tatagcaac gatatcatc gagttggat aagtttacc atgaaggag aagattctg gtgcagaat atcagcagg ccgttcaat gtgctggtg taccatcgg cagaggcaa ccagtggtt cctcatcct tgcaactct cactccgac tgttcgcat ctttgcatc tccaactgg aagcgcgac atcgccgtg aaaaagtgt ctctgcgct gccggttac caaatgacc gaaaacaac caatgtgta gtgaaacca agccaaaga tacctctta ttggccaaa ggcaaacca gcctcaatc aaaggaatc gaattggac agcaacaaa gaaacaatg atccctatc accggaatc aaccaacca atggcaata gactacgac gtcaaaacc ggcactatt atctactcc gacagccac cgaaaagta atcgaaggc gcgaaaatc aacgactcc tcgcacaaa accatcttc cagaaaaac gtccgcaga tgcgacggc ctggccttc gactggatg tcgcgcaac gtctactgg acggacgaa gaaatgggc agtatcggc gttttcaag ttggccaac tccagccaa aaccgaatt ttgctgcaa gacgctttc tacaaccct ttgtccata gtgctagat ccaggccgg ggtgtgatg tattgggcc gattggtcg agtatttac cccaacaaa gggaggatc cacgtggtt aatatggac ggaaaaaat ttgaaagat ttcgtggaa gttaacata gactggccg agtggtctc acgattgat ttcgtcgag aaacggttg tattggagc gaccgacat ttgcgtaaa atcgaaagt gtcgatttt aacggagtt aacaggaga gtcgagttg gcgaaagtg atggaaact ccgttcgga ttggcctta gggccgggg aaaactttg tattttact gaatttacc aaagggact gttatggcg tataataat aagacaggg tttcggcag ttggatcaa agcaatccg ccgattttt gatatcaag ctttatgat gcgacggct cagcaaggc gagaacgaa tgcaccaag aacacacca gattgcccc gaattgtgc ctcccgacg ccctcggac cccgtgtgc gagtgctcc agtggctac aaaaacgtc aacaagacg tgcgtgcga gatgtggcc ccaaatcaa gcgttcagt gtttgcggc cctcacgat ttccagtgt aaaaatttg aaatgtatt ccgtatcag cagacttgc gaccgtata gataactgt ggggaccaa tccgatgaa gctctaggt cctgacggt ccttgcaag gatgtcatt tgtccggcc aatcaaatc aaatgcgat aatcagact tgcatctcc aagtattgg gcttgcgat ggagagcaa gattgcgtt gacggaagc gacgaagat gagaaacgg tgttcggaa gtttgctca gcggctcag ttcaagtgt gcggtgtcg aaacggtgc atcccctcg gtgtggaag tgcgataat gtggcggat tgtgggccc gaagatatg agcgatgag gccgattgt gtgaaaaag cagtgcgaa gtgaacgaa ttcacgtgt gcgaatggc cgttgcatt tcgcaagtt ttgtattgt gacggtgtt gatgactgt aaggacagt tcggatgaa atcaactgc actgagtgt caagtcacg gagtttttc tgtccaagt actgcgact tgtcttcca aatagtaag aaatgtgac ggacagatt gattgtaac ggaggatac gatgaatat gagtgtaac gaaaatctg aactgtggg aaaacggag ttcaaatgc gccaacaat ttggagtgc atcccagag tcttatgtt tgcgacggc gacctggac tgtctcgac gccagcgac gagaaacac tgcaacaaa accgcccac cacaacacc acatcccca gccacctcg ccaacatgc caccacccg tcccgcttt tgcgacaac tccaccaaa tgcataacc gtcgaccac ttatgcgac aacatcgcc gattgttcc gacggttcg gacgaaggt ggccgttgt tccgaacaa ctttgcgac ctccgactc gattgctcc cactactgt cacaacgcc cccgaaggc ctgatctgc tcctgcccc cctgatttg cacctccaa cccgaccga attcactgc tccaaagcg cacccttgc gacacctgg ggcgtctgc tcacagaaa tgcatcgca gtgggcgaa cgctacaaa tgcggctgt ttcaaaggc tacgtcctc gaatcggat cgcttcacg tgcaagagc acaagtggc gccaccccg tatgtgatt ttcagtaat cggcatgaa ttgaaagca gtggagttg gttactttc aacgtcaag tcgtttatt tcaagtttg aagaatacg attgctttg gatttttat tacacgaag aatgcgaat atggtgttt tggaccgat gttattgac gataaaatc tacagaggg acagttgtt ggtggttct ttgagtaat atagaagtt gtggtgcag acaggactt tcaacggct gaaggcctt gctgttgac tggattggt gaaaatttg tattgggtt gaaagcaat cttgaccag atcgaggtg gctaaactt gatggtcga ttccggcga acgcttgtg gcaggtgag atggaaagt ccacgtgca gtagcagtg gacccacgt gatggttat ctcttctgg accgattgg gacaacaat gcgcctagg attgagcgc tgctctttg gctggacta gaccgggaa atagttgtg agagttgat aaaattgca gatgccggt tggcccaat ggtctaaca cttgattac gttctgaga cgcatttat tggattgac gctcgttct gactccatc cacacgact aaatacgac ggtagtgag caccatgaa gttatgcga aatcacgag acgctctcg catccgttc gccatagcg ctgtttgag aattacgtc tattggacg gattggagg acgaatagt gtggttagg gcgaataag tttacaggg ggcgatgtt gcggtaatt caaaggacg ttgacgcaa ccgtttgat atacagatt atgcatccg gcgcggcag ccgagagat gggcctaat ccgtgtggg gttaataat gggggctgt tcgcacttg tgtctgctg cacacgaat cggacgtac aggtgtgat tgtccgcat gtgatgagg ttgagtgcc gataataag acttgtgtg gtaaacgaa cgcgtgctg ctcatcgcc cgcacgagt gaaatccgc ggagtggac atcctccag ccctactac cacacaatc cccaccata agcgccccc caggtgctc aaccccgtc caactggag tacctcgcc cgtaacaac accctctac tgggccgac tcccaaatc agcgaaatc aaacgctcg ggcctcacc accggcccc atccaaacc ctcattgac accggcctc aaaaacccc tcaggacta gcaattgac tggttatcg aacctaatg ttcgtgtca tcacctcaa ggaataaca gtctcgaac ttagacgcc gagttcata accacaatc atcgataac ttaaacgtg ctatcagtg gctgcacat ccgcgtcta ggccaactg ttctggatc gccacaacc aacgagacc ttcattgag acgagcgca atggacgga agcaaacgc acgaaaatc gtgactgga ttacagtca cactcgcac agtctgtgt gtggatttg cacgcggat aagttgtat tgggtcagc gagtttgag atttatttc agtaatttg gacgggaag gatgtaaca aagttgaag ttgccgcat gtttcggtc cttgcagcg acagtttac caggaacat gtgtactat gcggacgat gacgatcat acgattcac attgttgat aaaatgacg ggcggtaat gacacgatt ttgcgcaac aatacgggg agtgttttc gcgttacgt atttacgac ccgatggaa cagaaaggt ttccaccct tgtgccaac tcgcagtgt tccgatctc tgtttaccg acgtctgcc acgactttc cgttgtcgc tgtgctacc ggttaccac accgaccca caaaacccc aacaagtgt gtgggagtc gaagagttt attttctat tcagtcaat tgggaaatc cgcgggttg gccctcaat ggcaacaac cagacggcc gttttaggg cccatctcg cgcatttcc atggcgaca gcgattgat ttcgtggcg gaatccgat ttgctgttc tgggctgat agtgacagg ggttccatc acgagtatc aagcgcgac gggacgcag agacggcac ataatcgag ccgactgag gccatggac agcgtgcca gttgattgg ctgacgggg ctggcggtt gactggatt gccgggaat atgtactgg tgtgattcg aaacgtggg gttattgag gtggcgagg cttgatggg agtaagcag cacgttctg ctggccaat gagattggg aagccgacg gcgatcgcg gtggatccg gccagaggg gtgatggtg tgggctggg gggcccagg ttggagatt gcgaccatg gatgggcgc aatcgcaac ttgttggtg aatagttcg gtggcgatt tccgatgtc acgcttgat tcggatcag aggaaggtg tatttttgt gatactagt aggcatacg attgaagtg atagggtat gatgggagt gggcatgag gttttggtt aatgatacg ctggagaat cccacggcg ttgaccatt ttggacgac aagttgtat tggcttgac aaaacgttc gagaagggc agcctcctc tcagccccc ttatccaac ctctccaac tacaaaatc ctcctccgc gacgttggc gacttcctc aaagacatc caaatcttc tcaaaacgc aaacaaacc ggcaccaac ccatgcgct caaaacaac ggcggttgc gaacaactc tgcctttac aacggaacc catcccgta tgcgtctgc tcgcacgga aaaatctcc tcagacggt aaaacatgc gaagctttc aaaaccttc gtcatgtat tcccgtgtc gtaagcatt gactcaatc caaatgctt gacgatgaa aacctccaa aacgcccct caccccagc atcaaaaac aacaccctt ttgaaaaat gccatcggc ttatcgttc agttacaaa caccaacgc ctcttctac tccgacata cagaagggc tcaatcaac gcagtttac tacaacggg actgatcac cgcattatc gtggagggc caaggctcg gtcgaaggc ctggcctac gaacaagtg cacaacatg ctctactgg acgtgcaac aaccatgcg accattaac cgggttaac ctaaccgac cagatgacc aactcgagt caagtcgag caaatcgtt cggctgcgt tcgcaagac aaaccgcgc gggattgct gtggacagt tgcggagcg cgcgttttc tggaccaat tggaattcg caccagccg agcatcgag cgcgtcttc ctatccggt tataaccgc gaggcgata atcaaaacc gacattcgc atgcccaac gcattgaca ctcgaccac aaagcgcaa aaactgtac tggggcgac gcccgactt gataaaatc gaacgttgt gaatatgac ggttcaaat cgtgtgatt ctggccaaa gtcacccca cagcacccg ttcgcccta gctgtgtac gccgattac atctactgg actgattgg atgctgcat gccgttcta cgggccgat aaactaacc ggccagaac gtggtttgg ttgagacgc gatgtggcc aggcctatg ggtatcgta accattgcc aacgacacc gaggactgt ttctacaac ccttgcaag attctgaac gggggctgt gaggagatt tgcactttg aacgcctcc gccatggtg cagtgctcc tgcatggac ggcaggatt ctggcagag gaccggaaa cgctgctat agcaacacg aaggcgtgc gatcagtac gacagtttc cggtgctcg gacgggggg tgtgttccg tttaagcta acgtgtgac ggtatagcg cactgttcg gacaaatcg gacgaagag ccgggctat tgcgggcac aggacgtgc ctccaaggc tggttccat tgcaacaac aagcgctgt gttgaacgg aaggataag tgcaacggg gtggacgat tgtggcgat gccagcgat gaggagaac tgctcgtgc agtgaggac gagtatttc cggtgcagt agcggggag tgcatccag aaggtgtta aggtgtgac aatgacccc gattgtgac gatgcgagt gacgagatg ggttgtgag gtgcgcaac tgtacgtta gatttccac gatggtaat atgattaat tgcgaaaat acaaccgcc tgtattcac aaggattgg ttctgtgat ggggaaaat gattgttgg gactgggct gatgagaag aattgcaca ggacgtgag aaacggtgc gatatattc cagtttcag tgctccaat ggcacgtgt attagtctg gaggcgaga tgtgacggg aaagatgac tgtcatgat gccaagtcg acgcgcgga ttgagttcg gatgaggag aattgtcga gttatggaa ggccactgc caaaacgac caattcatg tgtggcgac tcgcgttgc attccacta tcgtggcat tgcaacgga aaccccgac tgtcttgac aatagcgac gaatacgac tgccatcac caatgtcgc agcgaccag ttcaaatgc gacaacagc gagtgtatc cccctgagt tggcaatgt gacggccat cctgactgc atggaccag tccgacgaa agcaaacat tgcgaactt cgcgaatgc gaaaatggt gattttcgt tgcaacagc acaggtcgt tgtatctct cgtttgtgg ctgtgtgac ggcgaggct gattgtctg gacggcgcc gatgagcac aaggaccaa ggatgtggt gtttgcact tcggagcac ttccagtgc gttaacggt gtttgcatt aacaaaatg tattattgc gacggtgat aaggactgt aatgatggg agcgatgaa ccgccagag tgccacaag acttgcact tcggacgag ttcgcgtgc aataatggg aaatgcatc atggatctg ctgaagtgt gatgggaat gacgactgt ggtgatggt agcgatgaa gggaaggat tgccacaat gaaggcgat tattgcaag ggcaaaggg tggttccat tgcgggaat ggtgtttgc atcaatgat acgttgttg tgcaacggc gagaacaac tgtggggat ttcagcgac gaaacgaaa tgtcgcatc aacgaatgc acggcacag ccgccgccc tgctcgcaa aaatgtgtg gacaaaccg atcggttac gaatgtcag tgtcacacc ggttaccag accagcgca aaagacaag cacttgtgc gaagacatc aacgaatgt cttgcgaga ccttgcagt caactgtgt cgcaacacc agaggctca ttccattgc agttgtgcc cccggttat atactacac ccagacggt cgaacctgc ggttcaaat tcaattgtt ccggttaca cttatctta gctaaccgt tactacatt cgtgaaatt gatatgacg ggtcagtcg actctcttg gcacataat ctcacaaat gccgtggca ttggattat gactggagc tccaagtgc atttattgg tcggatgtg acacaatta gggagttcc attaagaga ttgtgcaat tataagaac agttcaagc gagattgag gttttgcat tcaccgact ttgcaaaat ccggatggt ttggccgtg gattgggtt ggacgaaac ctctactgg tgcgataag ggcttggac acgttggaa gtctcgtct ttggacggc cgtttccgg cgcgtgctc atctccaaa ggcttggag gagccccga gctgtggca ttggaccct ctccgtggt tacctctat tggaccgac tggggcacc catgcccac atcggcaaa gccggaatg gacggttcc aaccaacaa atcatcgta aattcaagc ctgggttgg cccaacgct ctcaccatc tcctacgaa accaaagaa ctgttctgg gccgatgcc cgtgaagat tatatcgca gtttcggac ctcgatggt cacaacatc caccgaatt atgagccga gagaaaaac cactacctg caactccac cacgtcttc gccatcgac atctgggaa gactacatt tactggacg gactgggag acgaaatcg gtcgaacgc tgtcacaaa tacaccgga aataactgc accaaaatc atgacgacg gtgcatcgt cccatggac atcagagtt gtgcatccg ttaaaacaa cccaagatt aaaaatccc tgcgagaag gctaattgc tccgctttg tgtctgtta acaccgcag gagccgttc tacaagtgc gtgtgtccg gagaattac gttttgaaa gaggatggg aaaagctgt gaggcgaat tgcacgtca gcgcatttc gagtgcaag aagagctac aaatgtatt ccgttttgg tggaaatgt gatacgcag gatgactgt ggagatggg agtgatgag ccgagtgat tgtcggccg ttcaagtgc atgccaggg cagtaccag tgcgataat gggcactgc acacatcct tcggacttg tgcaatggg aacgacgat tgcggggat cagtccgat gagaaggac tgcgagcat tacacgtgt ctgaacacg cagtttagg tgcccagga aacggcacg attgccccc agatgcatc ccctcgaaa ttccgctgc aacaaacac cccgactgc cccctcggc gaggacgag tcctcctgt cccccagcc acgtgcccc cccaaccaa ttcaaatgc gccaacgac aagtgcatc ccggccgtc tgggtctgc gacacggac aacgactgc ggcgacaac tccgacgag caacaggac tgccaatcg cgcacctgc tcgccccag cactaccgc tgcagctcg ggccgctgc atccccatg tcgtggcgc tgcgacggc gaccccgac tgcgccaac aacgaggac gagccgccg tcgtgcagc cagcccgag ttccacacg tgcgagccc acctacttc aagtgcaag aacaacaag tgcatcccg ggccgctgg cgctgcgac tacgacaac gactgcggc gacagctcc gacgaggtc gactgcgtg ccgcggaac tgctccgag tcggagttc cgctgcggg gacggccgc tgcatccgc ggcgcgcag aaatgcgac ggggagttc cagtgcgag gatcgctcg gacgaggcc aactgtcac acgcattgc aagaagaac gagttccag tgtgcgaac ccccaggtt tgcatctac ttggagtgg aagtgtgac ggcgaagcc gactgttcg gacggctca gatgaggcc aattgtagc gatacttgc ccggataac gggttcaag tgccacaat ggactgtgt attaacgag gattggaga tgtgacggc cagaaggat tgtgaggac ggttccgac gagatgttt tgttcgcta gttggttgt ttaccggga cgtttccgg tgcaagaac cacacttgt gtcccggtt agtttcctg tgtgacgga cacgaccag tgtgaggat gggagcgac gaagacccg cacatctgt cacaggttt aatatctgc cccccggat caattcact tgtaagaac ggtcactgc atcaaaaac tcactacgt tgtgacggt cggaacgac tgtagtgac aatagtgac gaagaaaat tgcaacaat tccacctgt aagtggaac acttgttcc caaatctgt attgaggcg aaacataac gtcccggtt tgtgactgg gcgaatcta gtgctcgtg tcggaagcc gagttgcga ttaatgtcg ccgtataaa ccaggcgac agcaataaa ttacgtagt aaaaccctg gccacagcc cccggttat aaagtcgat gctgttgat attctctac gggaggacg gaatcggtg gcgttctgg accgaccac cagaacaag cgcatccag agcgtggtt ttgcgcttg aatgagagc cgagctcgg cgcgattcc gacgtgcgg acggtcacg agtggtttg cacgacccc agagggatc agtgttgac tgggtggcg agacggttg tacgtcacg gatggggtc aggattttg gcttcgacc atcgatggg ttgtacgtt tacacggtc ctgagaggt gagatgaag caacccagg gatattgtg gtcgtgccc caggacggt gtgttgttt tggtcggac tggggaccg ccggcgcgg atcgaaacc gcgcacatg gacgggaat aagaggagg gttttggtg aatagtgcc attctgtac ccgacgggt ttggccgtg gattatacc acgaggagg ttgtattgg gccgatccg aagaccatg actgtggag acggtgaag tttgacggg tcggatagg catgtcgtg aggcatttt ggcaaggag atgaagccg tacaagatt gaggttttt gaaaattat ctctttgtt acgacgtat cagaagcac gatgtgtta cggttgaat aagtttggc aatgggaat gtcacgcat ttgtcgcaa gggttgact cgaattgcg gatattctt atacttcac gagcagaaa caggatcag agtgttaat aatacgtgc gttgatttt tgtcatcat acggaattc tgtttgttg agtccgaat acggctacg tgtacgtgc gccgatgga tacgtcaaa gataatctg acgtgtaaa gccgtcgcc agccgcacc cccaactgc cccctgaac tgcaacagc ggcacctgt aaaatcgtc caaggccaa aaccccaaa tgtttctgt ccggcccaa tacaccggc ccccgttgc gagcacttc cgttgctcc caatactgt aaaaacaaa ggcatgtgt tacgtcgac ttagccgcc ccccaatca cccgattcc ctgccccca ctgcgctgt aactgcgcc ccccaatgg accggcgaa cgctgcgaa acccccgtc aatctctgc gacggccgt tgctacaac ggtggcact tgtttcacc cccaaaccc gaaatgccc gtctgtaac tgtccctcg ggattcacc ggctcccgg tgccagaat tgcgcacaa ctcgtctgc caaaacggc ggcgtttgt gtcaaagat acgtgcaaa tgtccggtg ggttacagt ggacgccat tgcgaaatc tcattctgt gggaaaaac ggaaaaccg ataacgacc tcatcgggg ttgaaatgt agttgtctg cctggcttt ggcggcgag aaatgcgaa caggatcgg tgctaccaa cattgccag aacggcggc acttgccga atggggacg aagcaaccc gagtgcgat tgcccggcg tttttcggg gggaggcgt tgcgagatt gacttgtgc acaggcggg gaacccccg aaagggtgt gccaagaga tgcgtctgt gggaatggc ggcagttgt gttattatg aacggacgg ccggtgtgc aagtgccag aggccctgg ggtgggctc aagtgtgag atttacgtt ggcaacgct gatacgtgt gccgggctg tgccagaac gggggagtt tgccagatt ttgtcgccg catgtggac cccatttgc cagtgtccg cagtcgtgg acggggccc cggtgcaca cagttggcc gtctgtgcc aattactgc caaaatggg ggcacgtgt gacgtcagc gatgggctg ccgtactgt cgatgtcct gctgatttt gacggtgtt aagtgcgag ttgttggcc aagttggac gaagctgtg gcacataag gatgtaggg cgaggagtt gtagtcccg gttattgcc gcaattgtc gttattatt gtgattttg gttgttttc ctcgcattt gattacttc tacaggaag agacagtcg ttctcgcat gagaggtta caagagaac gatttcaac aatccgatg tatcaggat agggacgct gaaccgttc actttggac gctgataag tctggcaac tttgccaac cccgtatac gagtccgtc tacaacggc acgggcagc ggccggggc gaggaaaag gccgtgctt ttgcaacac ccagccgac gaggccccg acaccggcc cctgaggag atctag
- >TC009548 RA
tagaaaaga aacaggtga taaactgtc agagtgcga tagctcgac ggcgtgctg tccatcgag cgtttcttg ctccaactt cgttaattt gaatgaatt gtgtgaaaa accggacaa aaaccgcga atttccttt gattgtgtg cgatgacca ttgacaaaa atcgctttg ttctgcgat gttggtgaa aaagtgagt gaatctaga gtgccggca caatgaggt gcgattttt tgctgggac tgctgatga tggtctggg gggcccaag ggggcacca ccaccgctc cggccacca aaacagcat caacttgcg acagcgacc agttccaat gcctggacg gcccttgca ttccgtccc attggcgtt gcgacggcc agccagact gcgccgacg gcagcgacg agcccttcg agtgcatcc agacccaga cctgccgtc cagagcaat tccaatgtg ccctcacgc gaaagtgcc taccgttgg gttgggttt gcgacgaag accaggact gcgggacct cgacgacgc tgggcgtcg acacctccg acgaggacc ccagccagt gccacaagg agatcaaat gtccctgga accaggcca agtgcggct tcggcccag aatgcgccc caatcaaaa agttttgtg ataagcaga gggactgtg ttaacggag gcgacgaat gggattttt gccggaatt cgacgttta gctgtgata aattggtgt gcagctatg ggtgcagac ccacagggg agggaccca agtgcttct gtcccgagg gacagaggc ccgaaggga gcaggtgtg tggatgcgg acgaatgcg agctggaca acacttgcg ctcaagtgt gcaaaaata cagttgggt cgtttgagt gctcgtgcg tttcggggt atgagaaaa atgggacgg attgtgtcg cacttaatg ttcctccag ccctggacg cctcgatcg tcttctcaa cgcaggcgg aaatcgcgc gtttgaagc tctcaggcg aagcctggt cctcaaact cctccctcc agctcctca acagcacag ctttggagt ttgtcttca acaaccact caatttgct acgtccatc acaacttct ccaaagcgt cgctagttt gcgtcaaca tcgacgatt tctcgcaaa aaaccgaac taaaaaccc aatccactt tgctcgaat ttgaatccg tccaacaaa tggcaatgg attgggtgt ctcgtaatt ggtactttt tggacgacc agcgggaga ttttcctgg tttgttccg aacgtttgg agtggtgta acattttga tagagaacg atttgagca agcccagag ctctggctt tggacccga ctcgggggt acatgtttt tcaccaaat gggggcact ccccgccca tggtggagc ggtgtaaga tggacggga gcgagcgca aggctgtgg tgaaccaca agatcgtgt acccgtatg gggtgactg ttgattatc ccacgcagc atatttatt gggttgata cgtatctgg attttgttg agagagttg attacgacg ggaaggaca ggaagacga ttatgaggg gacttaagg tgcaaaatt tgtacggga tcagcgttt ttcaacgga agttgtatc tgtcgtcat ggtatagca acgatatca tcgagttgg ataagttta ccatgaagg agaagattc tggtgcaga atatcagca ggccgttca atgtgctgg tgtaccatc ggcagaggc aaccagtgg ttcctcatc cttgcaact ctcactccg actgttcgc atctttgca tctccaact ggaagcgcg acatcgccg tgaaaaagt gtctctgcg ctgccggtt accaaatga ccgaaaaca accaatgtg tagtgaaac caagccaaa gatacctct tattggcca aaggcaaac cagcctcaa tcaaaggaa tcgaattgg acagcaaca aagaaacaa tgatcccta tcaccggaa tcaaccaac caatggcaa tagactacg acgtcaaaa ccggcacta ttatctact ccgacagcc accgaaaag taatcgaag gcgcgaaaa tcaacgact cctcgcaca aaaccatct tccagaaaa acgtccgca gatgcgacg gcctggcct tcgactgga tgtcgcgca acgtctact ggacggacg aagaaatgg gcagtatcg gcgttttca agttggcca actccagcc aaaaccgaa ttttgctgc aagacgctt tctacaacc ctttgtcca tagtgctag atccaggcc ggggtgtga tgtattggg ccgattggt cgagtattt accccaaca aagggagga tccacgtgg ttaatatgg acggaaaaa atttgaaag atttcgtgg aagttaaca tagactggc cgagtggtc tcacgattg atttcgtcg agaaacggt tgtattgga gcgaccgac atttgcgta aaatcgaaa gtgtcgatt ttaacggag ttaacagga gagtcgagt tggcgaaag tgatggaaa ctccgttcg gattggcct tagggccgg ggaaaactt tgtatttta ctgaattta ccaaaggga ctgttatgg cgtataata ataagacag ggtttcggc agttggatc aaagcaatc cgccgattt ttgatatca agctttatg atgcgacgg ctcagcaag gcgagaacg aatgcacca agaacacac cagattgcc ccgaattgt gcctcccga cgccctcgg accccgtgt gcgagtgct ccagtggct acaaaaacg tcaacaaga cgtgcgtgc gagatgtgg ccccaaatc aagcgttca gtgtttgcg gccctcacg atttccagt gtaaaaatt tgaaatgta ttccgtatc agcagactt gcgaccgta tagataact gtggggacc aatccgatg aagctctag gtcctgacg gtccttgca aggatgtca tttgtccgg ccaatcaaa tcaaatgcg ataatcaga cttgcatct ccaagtatt gggcttgcg atggagagc aagattgcg ttgacggaa gcgacgaag atgagaaac ggtgttcgg aagtttgct cagcggctc agttcaagt gtgcggtgt cgaaacggt gcatcccct cggtgtgga agtgcgata atgtggcgg attgtgggc ccgaagata tgagcgatg aggccgatt gtgtgaaaa agcagtgcg aagtgaacg aattcacgt gtgcgaatg gccgttgca tttcgcaag ttttgtatt gtgacggtg ttgatgact gtaaggaca gttcggatg aaatcaact gcactgagt gtcaagtca cggagtttt tctgtccaa gtactgcga cttgtcttc caaatagta agaaatgtg acggacaga ttgattgta acggaggat acgatgaat atgagtgta acgaaaatc tgaactgtg ggaaaacgg agttcaaat gcgccaaca atttggagt gcatcccag agtcttatg tttgcgacg gcgacctgg actgtctcg acgccagcg acgagaaac actgcaaca aaaccgccc accacaaca ccacatccc cagccacct cgccaacat gccaccacc cgtcccgct tttgcgaca actccacca aatgcataa ccgtcgacc acttatgcg acaacatcg ccgattgtt ccgacggtt cggacgaag gtggccgtt gttccgaac aactttgcg acctccgac tcgattgct cccactact gtcacaacg cccccgaag gcctgatct gctcctgcc cccctgatt tgcacctcc aacccgacc gaattcact gctccaaag cgcaccctt gcgacacct ggggcgtct gctcacaga aatgcatcg cagtgggcg aacgctaca aatgcggct gtttcaaag gctacgtcc tcgaatcgg atcgcttca cgtgcaaga gcacaagtg gcgccaccc cgtatgtga ttttcagta atcggcatg aattgaaag cagtggagt tggttactt tcaacgtca agtcgttta tttcaagtt tgaagaata cgattgctt tggattttt attacacga agaatgcga atatggtgt tttggaccg atgttattg acgataaaa tctacagag ggacagttg ttggtggtt ctttgagta atatagaag ttgtggtgc agacaggac tttcaacgg ctgaaggcc ttgctgttg actggattg gtgaaaatt tgtattggg ttgaaagca atcttgacc agatcgagg tggctaaac ttgatggtc gattccggc gaacgcttg tggcaggtg agatggaaa gtccacgtg cagtagcag tggacccac gtgatggtt atctcttct ggaccgatt gggacaaca atgcgccta ggattgagc gctgctctt tggctggac tagaccggg aaatagttg tgagagttg ataaaattg cagatgccg gttggccca atggtctaa cacttgatt acgttctga gacgcattt attggattg acgctcgtt ctgactcca tccacacga ctaaatacg acggtagtg agcaccatg aagttatgc gaaatcacg agacgctct cgcatccgt tcgccatag cgctgtttg agaattacg tctattgga cggattgga ggacgaata gtgtggtta gggcgaata agtttacag ggggcgatg ttgcggtaa ttcaaagga cgttgacgc aaccgtttg atatacaga ttatgcatc cggcgcggc agccgagag atgggccta atccgtgtg gggttaata atgggggct gttcgcact tgtgtctgc tgcacacga atcggacgt acaggtgtg attgtccgc atgtgatga ggttgagtg ccgataata agacttgtg tggtaaacg aacgcgtgc tgctcatcg cccgcacga gtgaaatcc gcggagtgg acatcctcc agccctact accacacaa tccccacca taagcgccc cccaggtgc tcaaccccg tccaactgg agtacctcg cccgtaaca acaccctct actgggccg actcccaaa tcagcgaaa tcaaacgct cgggcctca ccaccggcc ccatccaaa ccctcattg acaccggcc tcaaaaacc cctcaggac tagcaattg actggttat cgaacctaa tgttcgtgt catcacctc aaggaataa cagtctcga acttagacg ccgagttca taaccacaa tcatcgata acttaaacg tgctatcag tggctgcac atccgcgtc taggccaac tgttctgga tcgccacaa ccaacgaga ccttcattg agacgagcg caatggacg gaagcaaac gcacgaaaa tcgtgactg gattacagt cacactcgc acagtctgt gtgtggatt tgcacgcgg ataagttgt attgggtca gcgagtttg agatttatt tcagtaatt tggacggga aggatgtaa caaagttga agttgccgc atgtttcgg tccttgcag cgacagttt accaggaac atgtgtact atgcggacg atgacgatc atacgattc acattgttg ataaaatga cgggcggta atgacacga ttttgcgca acaatacgg ggagtgttt tcgcgttac gtatttacg acccgatgg aacagaaag gtttccacc cttgtgcca actcgcagt gttccgatc tctgtttac cgacgtctg ccacgactt tccgttgtc gctgtgcta ccggttacc acaccgacc cacaaaacc ccaacaagt gtgtgggag tcgaagagt ttattttct attcagtca attgggaaa tccgcgggt tggccctca atggcaaca accagacgg ccgttttag ggcccatct cgcgcattt ccatggcga cagcgattg atttcgtgg cggaatccg atttgctgt tctgggctg atagtgaca ggggttcca tcacgagta tcaagcgcg acgggacgc agagacggc acataatcg agccgactg aggccatgg acagcgtgc cagttgatt ggctgacgg ggctggcgg ttgactgga ttgccggga atatgtact ggtgtgatt cgaaacgtg gggttattg aggtggcga ggcttgatg ggagtaagc agcacgttc tgctggcca atgagattg ggaagccga cggcgatcg cggtggatc cggccagag gggtgatgg tgtgggctg gggggccca ggttggaga ttgcgacca tggatgggc gcaatcgca acttgttgg tgaatagtt cggtggcga tttccgatg tcacgcttg attcggatc agaggaagg tgtattttt gtgatacta gtaggcata cgattgaag tgatagggt atgatggga gtgggcatg aggttttgg ttaatgata cgctggaga atcccacgg cgttgacca ttttggacg acaagttgt attggcttg acaaaacgt tcgagaagg gcagcctcc tctcagccc ccttatcca acctctcca actacaaaa tcctcctcc gcgacgttg gcgacttcc tcaaagaca tccaaatct tctcaaaac gcaaacaaa ccggcacca acccatgcg ctcaaaaca acggcggtt gcgaacaac tctgccttt acaacggaa cccatcccg tatgcgtct gctcgcacg gaaaaatct cctcagacg gtaaaacat gcgaagctt tcaaaacct tcgtcatgt attcccgtg tcgtaagca ttgactcaa tccaaatgc ttgacgatg aaaacctcc aaaacgccc ctcacccca gcatcaaaa acaacaccc ttttgaaaa atgccatcg gcttatcgt tcagttaca aacaccaac gcctcttct actccgaca tacagaagg gctcaatca acgcagttt actacaacg ggactgatc accgcatta tcgtggagg gccaaggct cggtcgaag gcctggcct acgaacaag tgcacaaca tgctctact ggacgtgca acaaccatg cgaccatta accgggtta acctaaccg accagatga ccaactcga gtcaagtcg agcaaatcg ttcggctgc gttcgcaag acaaaccgc gcgggattg ctgtggaca gttgcggag cgcgcgttt tctggacca attggaatt cgcaccagc cgagcatcg agcgcgtct tcctatccg gttataacc gcgaggcga taatcaaaa ccgacattc gcatgccca acgcattga cactcgacc acaaagcgc aaaaactgt actggggcg acgcccgac ttgataaaa tcgaacgtt gtgaatatg acggttcaa atcgtgtga ttctggcca aagtcaccc cacagcacc cgttcgccc tagctgtgt acgccgatt acatctact ggactgatt ggatgctgc atgccgttc tacgggccg ataaactaa ccggccaga acgtggttt ggttgagac gcgatgtgg ccaggccta tgggtatcg taaccattg ccaacgaca ccgaggact gtttctaca acccttgca agattctga acgggggct gtgaggaga tttgcactt tgaacgcct ccgccatgg tgcagtgct cctgcatgg acggcagga ttctggcag aggaccgga aacgctgct atagcaaca cgaaggcgt gcgatcagt acgacagtt tccggtgct cggacgggg ggtgtgttc cgtttaagc taacgtgtg acggtatag cgcactgtt cggacaaat cggacgaag agccgggct attgcgggc acaggacgt gcctccaag gctggttcc attgcaaca acaagcgct gtgttgaac ggaaggata agtgcaacg gggtggacg attgtggcg atgccagcg atgaggaga actgctcgt gcagtgagg acgagtatt tccggtgca gtagcgggg agtgcatcc agaaggtgt taaggtgtg acaatgacc ccgattgtg acgatgcga gtgacgaga tgggttgtg aggtgcgca actgtacgt tagatttcc acgatggta atatgatta attgcgaaa atacaaccg cctgtattc acaaggatt ggttctgtg atggggaaa atgattgtt gggactggg ctgatgaga agaattgca caggacgtg agaaacggt gcgatatat tccagtttc agtgctcca atggcacgt gtattagtc tggaggcga gatgtgacg ggaaagatg actgtcatg atgccaagt cgacgcgcg gattgagtt cggatgagg agaattgtc gagttatgg aaggccact gccaaaacg accaattca tgtgtggcg actcgcgtt gcattccac tatcgtggc attgcaacg gaaaccccg actgtcttg acaatagcg acgaatacg actgccatc accaatgtc gcagcgacc agttcaaat gcgacaaca gcgagtgta tccccctga gttggcaat gtgacggcc atcctgact gcatggacc agtccgacg aaagcaaac attgcgaac ttcgcgaat gcgaaaatg gtgattttc gttgcaaca gcacaggtc gttgtatct ctcgtttgt ggctgtgtg acggcgagg ctgattgtc tggacggcg ccgatgagc acaaggacc aaggatgtg gtgtttgca cttcggagc acttccagt gcgttaacg gtgtttgca ttaacaaaa tgtattatt gcgacggtg ataaggact gtaatgatg ggagcgatg aaccgccag agtgccaca agacttgca cttcggacg agttcgcgt gcaataatg ggaaatgca tcatggatc tgctgaagt gtgatggga atgacgact gtggtgatg gtagcgatg aagggaagg attgccaca atgaaggcg attattgca agggcaaag ggtggttcc attgcggga atggtgttt gcatcaatg atacgttgt tgtgcaacg gcgagaaca actgtgggg atttcagcg acgaaacga aatgtcgca tcaacgaat gcacggcac agccgccgc cctgctcgc aaaaatgtg tggacaaac cgatcggtt acgaatgtc agtgtcaca ccggttacc agaccagcg caaaagaca agcacttgt gcgaagaca tcaacgaat gtcttgcga gaccttgca gtcaactgt gtcgcaaca ccagaggct cattccatt gcagttgtg cccccggtt atatactac acccagacg gtcgaacct gcggttcaa attcaattg ttccggtta cacttatct tagctaacc gttactaca ttcgtgaaa ttgatatga cgggtcagt cgactctct tggcacata atctcacaa atgccgtgg cattggatt atgactgga gctccaagt gcatttatt ggtcggatg tgacacaat tagggagtt ccattaaga gattgtgca attataaga acagttcaa gcgagattg aggttttgc attcaccga ctttgcaaa atccggatg gtttggccg tggattggg ttggacgaa acctctact ggtgcgata agggcttgg acacgttgg aagtctcgt ctttggacg gccgtttcc ggcgcgtgc tcatctcca aaggcttgg aggagcccc gagctgtgg cattggacc ctctccgtg gttacctct attggaccg actggggca cccatgccc acatcggca aagccggaa tggacggtt ccaaccaac aaatcatcg taaattcaa gcctgggtt ggcccaacg ctctcacca tctcctacg aaaccaaag aactgttct gggccgatg cccgtgaag attatatcg cagtttcgg acctcgatg gtcacaaca tccaccgaa ttatgagcc gagagaaaa accactacc tgcaactcc accacgtct tcgccatcg acatctggg aagactaca tttactgga cggactggg agacgaaat cggtcgaac gctgtcaca aatacaccg gaaataact gcaccaaaa tcatgacga cggtgcatc gtcccatgg acatcagag ttgtgcatc cgttaaaac aacccaaga ttaaaaatc cctgcgaga aggctaatt gctccgctt tgtgtctgt taacaccgc aggagccgt tctacaagt gcgtgtgtc cggagaatt acgttttga aagaggatg ggaaaagct gtgaggcga attgcacgt cagcgcatt tcgagtgca agaagagct acaaatgta ttccgtttt ggtggaaat gtgatacgc aggatgact gtggagatg ggagtgatg agccgagtg attgtcggc cgttcaagt gcatgccag ggcagtacc agtgcgata atgggcact gcacacatc cttcggact tgtgcaatg ggaacgacg attgcgggg atcagtccg atgagaagg actgcgagc attacacgt gtctgaaca cgcagttta ggtgcccag gaaacggca cgattgccc ccagatgca tcccctcga aattccgct gcaacaaac accccgact gccccctcg gcgaggacg agtcctcct gtcccccag ccacgtgcc cccccaacc aattcaaat gcgccaacg acaagtgca tcccggccg tctgggtct gcgacacgg acaacgact gcggcgaca actccgacg agcaacagg actgccaat cgcgcacct gctcgcccc agcactacc gctgcagct cgggccgct gcatcccca tgtcgtggc gctgcgacg gcgaccccg actgcgcca acaacgagg acgagccgc cgtcgtgca gccagcccg agttccaca cgtgcgagc ccacctact tcaagtgca agaacaaca agtgcatcc cgggccgct ggcgctgcg actacgaca acgactgcg gcgacagct ccgacgagg tcgactgcg tgccgcgga actgctccg agtcggagt tccgctgcg gggacggcc gctgcatcc gcggcgcgc agaaatgcg acggggagt tccagtgcg aggatcgct cggacgagg ccaactgtc acacgcatt gcaagaaga acgagttcc agtgtgcga acccccagg tttgcatct acttggagt ggaagtgtg acggcgaag ccgactgtt cggacggct cagatgagg ccaattgta gcgatactt gcccggata acgggttca agtgccaca atggactgt gtattaacg aggattgga gatgtgacg gccagaagg attgtgagg acggttccg acgagatgt tttgttcgc tagttggtt gtttaccgg gacgtttcc ggtgcaaga accacactt gtgtcccgg ttagtttcc tgtgtgacg gacacgacc agtgtgagg atgggagcg acgaagacc cgcacatct gtcacaggt ttaatatct gccccccgg atcaattca cttgtaaga acggtcact gcatcaaaa actcactac gttgtgacg gtcggaacg actgtagtg acaatagtg acgaagaaa attgcaaca attccacct gtaagtgga acacttgtt cccaaatct gtattgagg cgaaacata acgtcccgg tttgtgact gggcgaatc tagtgctcg tgtcggaag ccgagttgc gattaatgt cgccgtata aaccaggcg acagcaata aattacgta gtaaaaccc tggccacag cccccggtt ataaagtcg atgctgttg atattctct acgggagga cggaatcgg tggcgttct ggaccgacc accagaaca agcgcatcc agagcgtgg ttttgcgct tgaatgaga gccgagctc ggcgcgatt ccgacgtgc ggacggtca cgagtggtt tgcacgacc ccagaggga tcagtgttg actgggtgg cgagacggt tgtacgtca cggatgggg tcaggattt tggcttcga ccatcgatg ggttgtacg tttacacgg tcctgagag gtgagatga agcaaccca gggatattg tggtcgtgc cccaggacg gtgtgttgt tttggtcgg actggggac cgccggcgc ggatcgaaa ccgcgcaca tggacggga ataagagga gggttttgg tgaatagtg ccattctgt acccgacgg gtttggccg tggattata ccacgagga ggttgtatt gggccgatc cgaagacca tgactgtgg agacggtga agtttgacg ggtcggata ggcatgtcg tgaggcatt ttggcaagg agatgaagc cgtacaaga ttgaggttt ttgaaaatt atctctttg ttacgacgt atcagaagc acgatgtgt tacggttga ataagtttg gcaatggga atgtcacgc atttgtcgc aagggttga ctcgaattg cggatattc ttatacttc acgagcaga aacaggatc agagtgtta ataatacgt gcgttgatt tttgtcatc atacggaat tctgtttgt tgagtccga atacggcta cgtgtacgt gcgccgatg gatacgtca aagataatc tgacgtgta aagccgtcg ccagccgca cccccaact gccccctga actgcaaca gcggcacct gtaaaatcg tccaaggcc aaaacccca aatgtttct gtccggccc aatacaccg gcccccgtt gcgagcact tccgttgct cccaatact gtaaaaaca aaggcatgt gttacgtcg acttagccg ccccccaat cacccgatt ccctgcccc cactgcgct gtaactgcg ccccccaat ggaccggcg aacgctgcg aaacccccg tcaatctct gcgacggcc gttgctaca acggtggca cttgtttca cccccaaac ccgaaatgc ccgtctgta actgtccct cgggattca ccggctccc ggtgccaga attgcgcac aactcgtct gccaaaacg gcggcgttt gtgtcaaag atacgtgca aatgtccgg tgggttaca gtggacgcc attgcgaaa tctcattct gtgggaaaa acggaaaac cgataacga cctcatcgg ggttgaaat gtagttgtc tgcctggct ttggcggcg agaaatgcg aacaggatc ggtgctacc aacattgcc agaacggcg gcacttgcc gaatgggga cgaagcaac ccgagtgcg attgcccgg cgtttttcg gggggaggc gttgcgaga ttgacttgt gcacaggcg gggaacccc cgaaagggt gtgccaaga gatgcgtct gtgggaatg gcggcagtt gtgttatta tgaacggac ggccggtgt gcaagtgcc agaggccct ggggtgggc tcaagtgtg agatttacg ttggcaacg ctgatacgt gtgccgggc tgtgccaga acgggggag tttgccaga ttttgtcgc cgcatgtgg accccattt gccagtgtc cgcagtcgt ggacggggc cccggtgca cacagttgg ccgtctgtg ccaattact gccaaaatg ggggcacgt gtgacgtca gcgatgggc tgccgtact gtcgatgtc ctgctgatt ttgacggtg ttaagtgcg agttgttgg ccaagttgg acgaagctg tggcacata aggatgtag ggcgaggag ttgtagtcc cggttattg ccgcaattg tcgttatta ttgtgattt tggttgttt tcctcgcat ttgattact tctacagga agagacagt cgttctcgc atgagaggt tacaagaga acgatttca acaatccga tgtatcagg atagggacg ctgaaccgt tcactttgg acgctgata agtctggca actttgcca accccgtat acgagtccg tctacaacg gcacgggca gcggccggg gcgaggaaa aggccgtgc ttttgcaac acccagccg acgaggccc cgacaccgg cccctgagg agatctagt gacatatcc cacaaaata tttaggttt attttcaat tgctgtgaa ttgtgccat ttattttaa aatatttcg gcagctgta tattatagc tgtacatat tattatctg atgtaattc ttcgatatt attgttgtt acatgaaat cttgcccga tggtgttaa cacacgaac cgaataacg tccttgtac atagaaatt atgtgactg atgataaat gatatattt tttaatgca ttgcttggt tgtgcagga acaaacgtt gtgctcaat actcatcta taaatgtat cgtaattat ttcttttta tgtcagatt ttataaaaa tg
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