Status
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Gene information
General information
TC001978 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450289.1: 65285..70509
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001978
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_11055

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001978 RA
      MATGLKKVG EIKLYIQDV EYTVKTDEV TPNTSLNSY 
      IRDTLQLTG TKSLCYEGG CGSCVVVLY NVDPTTEKD 
      IYLAVNSCL VPLLSCNGW RIYTIEGIG NPLSGYHPI 
      QEVLAKYNG TQCGFCSPG MVMNMYALY ESGKLTMEE 
      VENSFGGNI CRCTGYRPI LSAFKSLCT DASSEILGK 
      YPDIEDLRL CKDDKCGKK RITKCDREP FCLEFAESK 
      WIKVYTLQD LLTIMNQSK DLTYKLVGG NTAKGVFKS 
      YGTTVCVYI DVNSVPELK VQEVKDTTF VLGAGTSLT 
      TAMELFNQV GDKNSQFSY LKQLANHID LVANVPVRN 
      VGTLAGNLM MKHDIHDFP SDVFLILET IGATFTVVG 
      TDGKEVELT PHDFINYDM TLKVLKTIT FPSYPDTVK 
      YVSYKIMPR AQNSHAHVN AGFFFKFST DSILESATI 
      VYGNINPTF IHASESEKL LAGKNLFDN NTLQQVFAT 
      LSKELITDV IPPDPTPEF RKQLAIALF YKAVLTVAP 
      PDKLSPKNV SGGPVLTRP VSSGTQDYE TNESLYPLT 
      EAVPKLEAL AQTSGQAQY IHDMPEVPH QLHGTLILA 
      EAPPNSTIK TIDASKALE VEGIVAFYS KNDIPGDNN 
      FTPTDIFPA KEEIFCSGR VLYYEQPIG ILVGTNTSV 
      LKEAASLVE VTYDPPTVG PLLSVRQVL AAGRTDRIQ 
      EIKTITPTR KGDDVTHVV TGSFDIYHQ YHFHMETQC 
      CNVIPNENG LDVYPSSQW MDQIQSAIA RMLQIQNNK 
      INVTVRRLG GAFGAKISR NGLVSCAAA LAAWKLRQP 
      VKLSLSLQE NMVAIGKRW PLSTDYEVG VNDQGVIQY 
      LTCTHYSDL GAMVNEIGA EELLNLFTA NYVNDTFAI 
      HMDVVVTDT HTNTWARAP GSTEGLASI ESIMEHVAY 
      EIGVDPLDL RIANFPKDS QLLKYVNDL KTWADIEKR 
      KQEIATFNQ ENRWKKKGL SVVPMAYFL DVGGPFSVM 
      VSIFHGDGT VQISHGGIE VGQGINTKA AQVCAYKLG 
      IPLEKVAVL PSNSFIAPN NTTTGGSVT SEAVCYGVI 
      QACDQLLIR IQPYQDENP NGTWEDWIK ACFNDYVNL 
      SAIGLFSPN EPNVNTYLI YGVCATEVL VDVLTGQHI 
      ISRVDLIED TGQSMSPAI DIGQVEGAF VMGMGYYTT 
      EKIVYNYEG KILTNNTWT YYPPGPKDI PVDFRVKFP 
      KDNPNPVGV LKSKATAEP PLCMTISVP LAIRNAVAS 
      ARLDSGKAT SKWFPFDGT TNVENVFMN SKNDYAQYI 
      L
    CDS sequence
    • >TC001978 RA
      atggctact gggctaaaa aaggttggc gaaattaaa 
      ctgtatata caggatgtc gagtatacc gttaaaacc 
      gatgaagtc acccctaat acgtccctc aattcgtac 
      attcgtgac actctgcaa ttgactggg accaagtcc 
      ttatgctat gaagggggt tgtgggtct tgcgtcgtt 
      gtgctgtac aatgtggac cccacgacg gaaaaagac 
      atctacctg gcggtgaat tcgtgtttg gttccgctt 
      ttatcgtgt aatggctgg cgaatttac acaatcgaa 
      ggaatcgga aatccttta agcggttat cacccaatt 
      caagaagtt ctggcgaaa tataatggc acgcaatgc 
      ggtttttgt tcgcctgga atggtgatg aacatgtat 
      gcgttgtac gaatcgggg aaactaaca atggaagaa 
      gttgaaaat tcatttggt ggcaacata tgtcggtgt 
      accggttat agaccaata ctttccgct tttaaatcg 
      ctttgcaca gatgctagt tcggaaata cttggcaaa 
      tatcctgat atagaagac ttaagactg tgtaaggat 
      gataaatgt ggaaagaaa cgtataaca aaatgtgat 
      cgagagccg ttttgtttg gaatttgcg gaatcaaaa 
      tggattaaa gtttacact ttacaagat ttgctcact 
      atcatgaac cagtcgaaa gatctcact tataagcta 
      gtcgggggg aacacagcc aaaggagta tttaaatca 
      tatgggacc actgtatgt gtttacatc gacgtgaat 
      tcggtcccg gaactgaaa gttcaagaa gtcaaagac 
      acgactttt gtcttaggc gctggtact agtcttaca 
      acagcgatg gaacttttc aaccaagtt ggggataaa 
      aactcgcaa ttctcttac ttaaaacaa ttagctaat 
      catattgac ttagtcgca aacgtccct gtgaggaat 
      gtgggaacg ctggccgga aatctaatg atgaaacac 
      gacattcac gatttccca tccgatgtt tttctcata 
      ttggaaaca atcggtgcc acgtttaca gttgttgga 
      actgatgga aaagaagtt gagctaacc cctcatgac 
      tttattaat tacgatatg acgctgaaa gttttaaaa 
      actatcact tttcccagc tatccagac acggttaag 
      tacgttagt tataaaatc atgccgagg gcccaaaat 
      tcgcacgcg catgtaaat gccgggttt ttcttcaaa 
      ttcagtact gattcgata cttgagtct gcgactatc 
      gtgtacgga aacataaat ccgacgttt attcacgca 
      tctgagtcg gaaaagctt ctagcaggg aaaaacttg 
      ttcgataat aacactctt cagcaagtt tttgcaacc 
      ctttccaag gaattgatc accgatgtt attccaccg 
      gacccgaca ccggaattt aggaaacaa ctagcaatt 
      gcacttttc tacaaagca gtattaaca gttgctccg 
      cctgataaa ctatccccc aaaaatgta tcaggaggt 
      cctgtgctc acaagacca gtttcttca gggacacaa 
      gactacgaa accaatgag tcattgtat ccactcacc 
      gaagccgtg ccaaaattg gaagcactt gcccaaact 
      tctggccaa gcgcagtac atccacgat atgccagaa 
      gtgcctcat cagttgcac ggcactttg atcctggct 
      gaagcgcca ccaaattcc acaataaaa acaattgac 
      gcaagcaaa gctttggaa gtcgaaggc attgttgcg 
      ttttacagc aagaacgat attcccgga gacaataat 
      ttcaccccc actgacata tttccagca aaggaagaa 
      atattttgc agcggccgt gtcctgtat tacgaacaa 
      ccgatcgga attttggtc ggaactaac accagcgtt 
      ctgaaagag gccgcaagt ttggttgaa gtgacgtac 
      gatccccca actgtgggt cctctcctg tcagttcgt 
      caggttctg gcagctggt cgaactgac cgcatccaa 
      gaaatcaaa acaataaca ccaacacga aaaggcgac 
      gatgtcaca cacgtggtg actgggtca ttcgatatc 
      taccaccag taccacttc cacatggag acgcaatgc 
      tgcaacgtg atacccaac gagaatggg ttggacgtt 
      tacccgtcg tcccagtgg atggaccag attcagtca 
      gccatcgcc cgcatgctc caaatccag aacaacaaa 
      attaacgta acagttcgg agactcggc ggcgccttt 
      ggtgccaaa atcagcaga aacggctta gtgtcctgt 
      gcggccgcc ttggctgcg tggaaactg agacagccg 
      gtgaaattg tccctctcg ctgcaggaa aacatggtg 
      gcaattggg aaaaggtgg ccgctttct accgactac 
      gaagtggga gtcaacgac caaggagtc atccagtat 
      ttgacttgc acccactac tccgattta ggagccatg 
      gttaacgaa atcggagcc gaagagtta ctcaacctg 
      ttcacggct aattacgtt aatgacact tttgccata 
      cacatggac gtcgtcgtg acagacacg cacacgaac 
      acgtgggct cgagctcca ggctcgacc gaaggcttg 
      gcctccatt gaatcgata atggagcat gtggcttac 
      gaaatcgga gtggatcct ctcgatttg cggatcgca 
      aattttccg aaagactcc cagcttttg aaatacgtc 
      aacgaccta aaaacctgg gctgatata gagaaaagg 
      aagcaggaa attgcgact ttcaaccag gaaaatcgg 
      tggaaaaag aaaggttta tccgtggta ccaatggcg 
      tattttttg gacgttgga ggcccgttt tctgtgatg 
      gtttcaatt ttccacggc gatggtact gtgcaaatt 
      tcccacggt gggattgaa gtcgggcaa gggatcaac 
      accaaggcg gcacaagtg tgtgcttac aaacttggg 
      attccgtta gaaaaagtt gcggttttg cctagcaat 
      agttttatt gcgccaaat aacacgaca actggcggt 
      agtgtcact tcagaggcc gtttgctac ggcgtgatt 
      caagcttgc gatcagtta ttaattcgg atccaaccg 
      tatcaagac gaaaatcct aacgggact tgggaggac 
      tggattaag gcttgtttt aatgattat gtcaattta 
      tcggcaatt ggattgttt tcgcctaac gagccaaat 
      gtgaatacg tatctaatt tatggagtt tgtgcgact 
      gaagtactt gtcgatgtt ttaaccgga caacatata 
      atttcaaga gtcgattta attgaagat acgggacaa 
      tcaatgagt cctgctatt gacataggt caagtcgag 
      ggtgctttc gtgatggga atgggttat tatacgacc 
      gaaaaaatt gtttacaat tacgagggc aagatttta 
      accaataac acttggact tattatccg ccaggaccg 
      aaagatatt ccggtcgat tttcgcgta aaattcccg 
      aaggataat ccgaatcct gtgggtgta ttgaagtcg 
      aaagcgact gcggaacct cctttgtgt atgacgata 
      tcggttcca ttggcaatt cgaaatgcc gtagcttcg 
      gccagattg gattcgggg aaagctact tcaaaatgg 
      tttcctttt gacgggact acgaatgtg gagaacgtt 
      tttatgaac agcaagaat gattacgcg caatatatt 
      ttgtga
    mRNA sequence
    • >TC001978 RA
      ggtgagtga ctaactata aacctagta atcaccttg 
      tatccaata tgttaaact tggactaaa atattaatt 
      tcgataaac tggttttaa aatggctac tgggctaaa 
      aaaggttgg cgaaattaa actgtatat acaggatgt 
      cgagtatac cgttaaaac cgatgaagt cacccctaa 
      tacgtccct caattcgta cattcgtga cactctgca 
      attgactgg gaccaagtc cttatgcta tgaaggggg 
      ttgtgggtc ttgcgtcgt tgtgctgta caatgtgga 
      ccccacgac ggaaaaaga catctacct ggcggtgaa 
      ttcgtgttt ggttccgct tttatcgtg taatggctg 
      gcgaattta cacaatcga aggaatcgg aaatccttt 
      aagcggtta tcacccaat tcaagaagt tctggcgaa 
      atataatgg cacgcaatg cggtttttg ttcgcctgg 
      aatggtgat gaacatgta tgcgttgta cgaatcggg 
      gaaactaac aatggaaga agttgaaaa ttcatttgg 
      tggcaacat atgtcggtg taccggtta tagaccaat 
      actttccgc ttttaaatc gctttgcac agatgctag 
      ttcggaaat acttggcaa atatcctga tatagaaga 
      cttaagact gtgtaagga tgataaatg tggaaagaa 
      acgtataac aaaatgtga tcgagagcc gttttgttt 
      ggaatttgc ggaatcaaa atggattaa agtttacac 
      tttacaaga tttgctcac tatcatgaa ccagtcgaa 
      agatctcac ttataagct agtcggggg gaacacagc 
      caaaggagt atttaaatc atatgggac cactgtatg 
      tgtttacat cgacgtgaa ttcggtccc ggaactgaa 
      agttcaaga agtcaaaga cacgacttt tgtcttagg 
      cgctggtac tagtcttac aacagcgat ggaactttt 
      caaccaagt tggggataa aaactcgca attctctta 
      cttaaaaca attagctaa tcatattga cttagtcgc 
      aaacgtccc tgtgaggaa tgtgggaac gctggccgg 
      aaatctaat gatgaaaca cgacattca cgatttccc 
      atccgatgt ttttctcat attggaaac aatcggtgc 
      cacgtttac agttgttgg aactgatgg aaaagaagt 
      tgagctaac ccctcatga ctttattaa ttacgatat 
      gacgctgaa agttttaaa aactatcac ttttcccag 
      ctatccaga cacggttaa gtacgttag ttataaaat 
      catgccgag ggcccaaaa ttcgcacgc gcatgtaaa 
      tgccgggtt tttcttcaa attcagtac tgattcgat 
      acttgagtc tgcgactat cgtgtacgg aaacataaa 
      tccgacgtt tattcacgc atctgagtc ggaaaagct 
      tctagcagg gaaaaactt gttcgataa taacactct 
      tcagcaagt ttttgcaac cctttccaa ggaattgat 
      caccgatgt tattccacc ggacccgac accggaatt 
      taggaaaca actagcaat tgcactttt ctacaaagc 
      agtattaac agttgctcc gcctgataa actatcccc 
      caaaaatgt atcaggagg tcctgtgct cacaagacc 
      agtttcttc agggacaca agactacga aaccaatga 
      gtcattgta tccactcac cgaagccgt gccaaaatt 
      ggaagcact tgcccaaac ttctggcca agcgcagta 
      catccacga tatgccaga agtgcctca tcagttgca 
      cggcacttt gatcctggc tgaagcgcc accaaattc 
      cacaataaa aacaattga cgcaagcaa agctttgga 
      agtcgaagg cattgttgc gttttacag caagaacga 
      tattcccgg agacaataa tttcacccc cactgacat 
      atttccagc aaaggaaga aatattttg cagcggccg 
      tgtcctgta ttacgaaca accgatcgg aattttggt 
      cggaactaa caccagcgt tctgaaaga ggccgcaag 
      tttggttga agtgacgta cgatccccc aactgtggg 
      tcctctcct gtcagttcg tcaggttct ggcagctgg 
      tcgaactga ccgcatcca agaaatcaa aacaataac 
      accaacacg aaaaggcga cgatgtcac acacgtggt 
      gactgggtc attcgatat ctaccacca gtaccactt 
      ccacatgga gacgcaatg ctgcaacgt gatacccaa 
      cgagaatgg gttggacgt ttacccgtc gtcccagtg 
      gatggacca gattcagtc agccatcgc ccgcatgct 
      ccaaatcca gaacaacaa aattaacgt aacagttcg 
      gagactcgg cggcgcctt tggtgccaa aatcagcag 
      aaacggctt agtgtcctg tgcggccgc cttggctgc 
      gtggaaact gagacagcc ggtgaaatt gtccctctc 
      gctgcagga aaacatggt ggcaattgg gaaaaggtg 
      gccgctttc taccgacta cgaagtggg agtcaacga 
      ccaaggagt catccagta tttgacttg cacccacta 
      ctccgattt aggagccat ggttaacga aatcggagc 
      cgaagagtt actcaacct gttcacggc taattacgt 
      taatgacac ttttgccat acacatgga cgtcgtcgt 
      gacagacac gcacacgaa cacgtgggc tcgagctcc 
      aggctcgac cgaaggctt ggcctccat tgaatcgat 
      aatggagca tgtggctta cgaaatcgg agtggatcc 
      tctcgattt gcggatcgc aaattttcc gaaagactc 
      ccagctttt gaaatacgt caacgacct aaaaacctg 
      ggctgatat agagaaaag gaagcagga aattgcgac 
      tttcaacca ggaaaatcg gtggaaaaa gaaaggttt 
      atccgtggt accaatggc gtatttttt ggacgttgg 
      aggcccgtt ttctgtgat ggtttcaat tttccacgg 
      cgatggtac tgtgcaaat ttcccacgg tgggattga 
      agtcgggca agggatcaa caccaaggc ggcacaagt 
      gtgtgctta caaacttgg gattccgtt agaaaaagt 
      tgcggtttt gcctagcaa tagttttat tgcgccaaa 
      taacacgac aactggcgg tagtgtcac ttcagaggc 
      cgtttgcta cggcgtgat tcaagcttg cgatcagtt 
      attaattcg gatccaacc gtatcaaga cgaaaatcc 
      taacgggac ttgggagga ctggattaa ggcttgttt 
      taatgatta tgtcaattt atcggcaat tggattgtt 
      ttcgcctaa cgagccaaa tgtgaatac gtatctaat 
      ttatggagt ttgtgcgac tgaagtact tgtcgatgt 
      tttaaccgg acaacatat aatttcaag agtcgattt 
      aattgaaga tacgggaca atcaatgag tcctgctat 
      tgacatagg tcaagtcga gggtgcttt cgtgatggg 
      aatgggtta ttatacgac cgaaaaaat tgtttacaa 
      ttacgaggg caagatttt aaccaataa cacttggac 
      ttattatcc gccaggacc gaaagatat tccggtcga 
      ttttcgcgt aaaattccc gaaggataa tccgaatcc 
      tgtgggtgt attgaagtc gaaagcgac tgcggaacc 
      tcctttgtg tatgacgat atcggttcc attggcaat 
      tcgaaatgc cgtagcttc ggccagatt ggattcggg 
      gaaagctac ttcaaaatg gtttccttt tgacgggac 
      tacgaatgt ggagaacgt ttttatgaa cagcaagaa 
      tgattacgc gcaatatat tttgtgaag attttattg 
      gttatgtaa atttgcgta aaataaatt acttcataa 
      tccgtttat tttctttag gcaaatttc gaaagattc 
      gaaagtatt aaacctccc aacatcttc atccgagcg 
      caccctgac atcgggcat tttccgtat tctctcaac 
      ttgacttcc atcaatatt caattcgag acaacatct 
      ttccattag ggttctata agacaagtt gtgagaatc 
      catttctat ttcgatgta gtccgcatc ggcaccggc 
      aatccgaat tttgaaaac atgtttacc taatgaaag 
      agctggtca agcgagtgt ccgatatgt gattttatt 
      tattacctg gggctggtc gatacattt cggtggaac 
      tgttttacg acaacatac gagcgtaca aaagacaac 
      gtaaaactg acaatacca gcgctatca tctttttat 
      aaaggct
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