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Gene information
General information
TC003130 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1336730..1343555
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003130
    iBeetle screen iB_11054

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003130 RA
      MSWFDASGF ASIAKSALR EAQRTIDKA LDIKEDEVN 
      VVPTNTPID TNSDDFFGT WGISQSGHI KEGKKDEEG 
      PKMAKLQSS LWGSFTGSF FDASKEYPK TPSIDSLDD 
      SVDIGSEHF SRSKLVVQQ SDDGESHYS RLSSMETDE 
      GTLVNDNEG KLVEIQLDS PDGPSNSDQ AVVVKRPEK 
      TQSSIINRL SAISNESGK NSSESVELI TCSTECTTS 
      PESELLSGE HSISTSSSA VAKQTSESV EIIADSLTS 
      PSSVEIIGS DSIDCKNQD EFASPMESP IAEEEASPC 
      AEKNTPDSV EVIPEDQEE SVAEDTMSY TSISESTSA 
      TVLDQAFLH LKPQKMLKD TYNTSMPES DIPLSPEKN 
      HTFADAITR APSRSGMHL PLTQVNQVL IDTQPQSSR 
      QDLSNLLKN TNIIDIPQE DNVEGQIES CDEGSQSDR 
      TVIAKDGGM DSSSDTSVT ENSVYLKNL IADAMTEKS 
      PEVPDCATK TSHFMDLTQ SMIDSSQGN VVLENISIS 
      QLDMPPREN SPVSSESRS DLVKIGSSG HTSGDELET 
      TTSSDIEII SSPNGDSSS TQSRQSPAK QTCTKNKSD 
      GTKVDLLCK MTMKKAKGH TRELSETSS VSDDSHSSE 
      VDRLIKRIA EMTEILESR ESKLIDINR RNAELQELN 
      TNLRHQLDS MLTKQLETA DLSQVTEEY TQRLSALEK 
      KFQQAIREK DTLRKQLEQ SKQEAATRL SKSDLDSLI 
      SEKDEIIKE LREEGEKLS KQQLQHSNI IKKLRAKEK 
      ENESTIKHL KETIEDLSS EADRLKRSL TAKEEVERS 
      QIEAVHQLT AKNKKLETE VDKFRSQLD DLTQKYDTV 
      KKSLDAAKK ELVDKNKTS SELIAREHK LESLENEKK 
      QTESQNAAI LNELEELRS KMRQLDLDY AKKEQSLRK 
      ENNDLLRRL EDAEARNEE LSQSVLEVS KPLVRQLES 
      LQATHTMKI ASFERIEQE MTLKINELQ TRLQTSLNS 
      ERTVKDESV TLKTRLSDL ESELSSLKH QNELLRVEV 
      EQNKTEKQI SEQELGREI NELKEQLVS ERHKVAELA 
      QNASSLQEQ LEKSASESE RKKSTEDAP NLGRNSPRT 
      ASNSPTLSL GKISVAESL GSSFWSQDE PFDGGQPPR 
      YTNMFEMQM LQTNLKQRD GELQQLQWE LNRREQERA 
      LLNKEISSL LTRVEELET KVQEYDVLK GQHSELQQQ 
      YDTLCQLFG EKVEENEEL KLDLQDVKE MYKSQIDEL 
      LKQQKQNL
    CDS sequence
    • >TC003130 RA
      atgagttgg tttgatgcg tcaggcttt gctagcata 
      gcaaaatcc gccctcagg gaagcgcag agaacgata 
      gacaaggct ttagatatc aaagaagac gaagtaaac 
      gtggttcct acaaacacg cccattgac acaaactct 
      gatgatttt ttcggcacg tggggcatt agtcagtct 
      ggccatatc aaagagggt aaaaaggat gaagaaggg 
      cctaaaatg gccaagctc cagtccagc ctgtggggc 
      tcattcacc ggctcattt ttcgacgcc agtaaagag 
      taccctaag acaccttcc attgatagt ctagacgat 
      tcagttgac ataggaagt gagcatttt agcagaagc 
      aaacttgtg gtgcagcaa agtgacgac ggggagagc 
      cattactcg cgcctgtcg tcaatggaa acagatgag 
      gggaccctt gtcaatgac aatgaaggc aaactagtg 
      gaaattcag ttagattca cctgacggg ccttcaaac 
      tctgaccaa gccgttgtg gtcaaaagg ccagagaag 
      acgcaaagt tcgataatt aaccgccta tcggcaatt 
      agcaatgag agtggaaaa aacagttct gaaagtgtg 
      gagctaatc acttgcagt acggaatgc accactagt 
      ccagaatcg gagcttttg tcaggggaa cacagcatt 
      tcaacttcg agctctgct gtagctaaa cagacttca 
      gagtcggtt gaaattata gcagatagc ttaactagt 
      cccagttcg gttgaaata attgggtca gatagcata 
      gactgtaag aatcaagat gaatttgca tcgccaatg 
      gaatcgcca attgcagaa gaggaggcc agcccatgt 
      gcggaaaag aacactccc gacagcgtg gaagtaatc 
      cccgaagac caagaagag agcgtcgct gaagacaca 
      atgtcgtac acatcgatt tccgaaagc acctctgcc 
      acagtcctc gaccaagca ttcctccac ctcaaaccc 
      caaaaaatg ctaaaagac acctataac acatcaatg 
      cccgaaagc gacattcca ctcagtccc gaaaaaaac 
      cacactttc gccgatgca atcacaaga gcacccagt 
      cgaagtggc atgcattta cctctaaca caagtcaac 
      caagtacta atagacacc cagccacag agcagcaga 
      caggacttg tccaacctt ttgaaaaac accaacatt 
      atcgatata cctcaggaa gacaacgtg gaaggacaa 
      atcgaatcc tgtgatgaa ggttcccaa tctgaccgc 
      actgtaata gcaaaagac ggcggaatg gacagtagt 
      agcgacaca agtgtgacg gaaaattcc gtttatttg 
      aaaaattta atcgctgat gccatgact gaaaaaagc 
      ccggaagtg cccgattgt gcaacgaaa accagccat 
      tttatggat ttgactcag agtatgatc gactcaagt 
      cagggaaat gtggttttg gagaacatt tccataagt 
      cagttggat atgccgcct cgggaaaat tcgccagtc 
      agttcggaa agtcgctcc gatttggtc aaaatcggg 
      tcttcggga cacacctct ggtgatgaa ttggaaaca 
      accacctca tcagatatt gaaattatt tcaagtccg 
      aacggagat tcgagtagt acgcaaagt aggcaaagc 
      ccagccaag caaacttgc acgaaaaat aaatcagac 
      ggcaccaaa gtcgatctt ttgtgcaaa atgacgatg 
      aaaaaagcc aaaggccac actagagaa ttgtcggaa 
      acttcgagc gtgagcgat gattcgcat tcatccgaa 
      gttgatcgg cttatcaaa aggattgcg gaaatgacc 
      gaaattttg gagtccaga gaatcgaaa ttaattgat 
      atcaatagg agaaatgcc gaattgcaa gagttgaac 
      accaacttg cgacatcag ctcgattcg atgcttacc 
      aagcaattg gaaactgca gatttgtct caagtgact 
      gaagaatat acccagcgt ttgtccgcc ttagagaaa 
      aagtttcaa caagcaatt cgggaaaaa gacacgctg 
      aggaaacaa ctggaacag agcaaacag gaggctgcc 
      actaggctc agcaaaagc gatttggac tcacttata 
      agcgaaaaa gatgaaatt attaaggag ttgcgtgag 
      gagggggaa aagttgtcc aaacagcag ttgcaacat 
      tcgaatatt atcaagaag ttgagggcg aaagaaaag 
      gaaaatgag tcaacaatc aaacattta aaagaaaca 
      attgaggac ttgtcgtct gaagctgac cgtttaaag 
      cgttcacta accgcaaaa gaggaagtt gaaaggtcg 
      caaatcgaa gcagttcac cagctaaca gcaaaaaat 
      aaaaaacta gaaactgaa gttgataaa ttccgaagt 
      cagctcgac gacctcaca caaaagtat gacaccgtg 
      aagaagtct ttagacgct gcgaaaaaa gaactcgtc 
      gacaagaac aagactagt tctgagtta atcgcacgc 
      gagcataaa ttggagagt ttagagaac gaaaaaaag 
      caaacggag tcacaaaac gcagcgatt cttaacgaa 
      ctggaggaa ctacggtca aaaatgcga caactcgat 
      ttagactac gcgaaaaaa gaacagtcg ctacgtaaa 
      gaaaacaac gatcttttg aggcgtttg gaggacgct 
      gaggccaga aacgaagaa ttatcacaa tccgttctt 
      gaagttagt aagcctttg gtgagacaa ttggagtcg 
      ttacaagca acacatacg atgaaaatc gccagtttt 
      gaaagaatt gaacaagaa atgacgctt aaaataaat 
      gagttgcaa actagatta cagacgtcc cttaactca 
      gaacggacg gtcaaagac gagtctgtg actctaaaa 
      actagactt tcagatttg gaatcggaa ctaagttcg 
      cttaagcac caaaacgag ctgcttcga gtggaagtg 
      gagcagaat aaaaccgaa aaacaaatc tccgaacag 
      gagttaggt cgtgaaatt aacgaattg aaggaacag 
      ttagtgtct gagagacac aaagtggcc gagttggcg 
      caaaacgcg tccagtttg caagaacag ctggagaaa 
      tcagcgagt gaatccgag cgcaagaaa agcactgaa 
      gatgcgcca aatttgggc cgaaactcg ccgcgcact 
      gcgagcaat tcgccaact ttaagtctg gggaaaatt 
      tccgtggcg gaatcgctt ggaagctcg ttttggtcg 
      caggatgag ccctttgac gggggacaa ccgccaagg 
      tacaccaac atgttcgag atgcaaatg cttcaaacc 
      aacttgaaa cagcgcgac ggtgaattg caacaattg 
      cagtgggag ttgaaccgg agggaacaa gagagggcg 
      ctcttgaat aaagaaatc tcgtcgctt ttgacccgt 
      gttgaggaa cttgaaacc aaagtccag gaatacgat 
      gttttaaaa ggacaacat agtgagcta cagcaacag 
      tatgatact ctttgccag ttgtttggc gagaaagtt 
      gaagaaaac gaagaactg aagcttgat ttgcaagac 
      gtgaaagag atgtacaaa agtcagatt gatgagttg 
      ttgaaacag caaaaacag aacttgtaa
    mRNA sequence
    • >TC003130 RA
      aaagatgaa acgtcaaca tgtcatagt tgtcaaaaa 
      ttaaaaaca atgccttta aatggaata attcgtgac 
      aacgacccg atatttcgc taaatttta gtagagcca 
      acgatgtaa aatgagttg gtttgatgc gtcaggctt 
      tgctagcat agcaaaatc cgccctcag ggaagcgca 
      gagaacgat agacaaggc tttagatat caaagaaga 
      cgaagtaaa cgtggttcc tacaaacac gcccattga 
      cacaaactc tgatgattt tttcggcac gtggggcat 
      tagtcagtc tggccatat caaagaggg taaaaagga 
      tgaagaagg gcctaaaat ggccaagct ccagtccag 
      cctgtgggg ctcattcac cggctcatt tttcgacgc 
      cagtaaaga gtaccctaa gacaccttc cattgatag 
      tctagacga ttcagttga cataggaag tgagcattt 
      tagcagaag caaacttgt ggtgcagca aagtgacga 
      cggggagag ccattactc gcgcctgtc gtcaatgga 
      aacagatga ggggaccct tgtcaatga caatgaagg 
      caaactagt ggaaattca gttagattc acctgacgg 
      gccttcaaa ctctgacca agccgttgt ggtcaaaag 
      gccagagaa gacgcaaag ttcgataat taaccgcct 
      atcggcaat tagcaatga gagtggaaa aaacagttc 
      tgaaagtgt ggagctaat cacttgcag tacggaatg 
      caccactag tccagaatc ggagctttt gtcagggga 
      acacagcat ttcaacttc gagctctgc tgtagctaa 
      acagacttc agagtcggt tgaaattat agcagatag 
      cttaactag tcccagttc ggttgaaat aattgggtc 
      agatagcat agactgtaa gaatcaaga tgaatttgc 
      atcgccaat ggaatcgcc aattgcaga agaggaggc 
      cagcccatg tgcggaaaa gaacactcc cgacagcgt 
      ggaagtaat ccccgaaga ccaagaaga gagcgtcgc 
      tgaagacac aatgtcgta cacatcgat ttccgaaag 
      cacctctgc cacagtcct cgaccaagc attcctcca 
      cctcaaacc ccaaaaaat gctaaaaga cacctataa 
      cacatcaat gcccgaaag cgacattcc actcagtcc 
      cgaaaaaaa ccacacttt cgccgatgc aatcacaag 
      agcacccag tcgaagtgg catgcattt acctctaac 
      acaagtcaa ccaagtact aatagacac ccagccaca 
      gagcagcag acaggactt gtccaacct tttgaaaaa 
      caccaacat tatcgatat acctcagga agacaacgt 
      ggaaggaca aatcgaatc ctgtgatga aggttccca 
      atctgaccg cactgtaat agcaaaaga cggcggaat 
      ggacagtag tagcgacac aagtgtgac ggaaaattc 
      cgtttattt gaaaaattt aatcgctga tgccatgac 
      tgaaaaaag cccggaagt gcccgattg tgcaacgaa 
      aaccagcca ttttatgga tttgactca gagtatgat 
      cgactcaag tcagggaaa tgtggtttt ggagaacat 
      ttccataag tcagttgga tatgccgcc tcgggaaaa 
      ttcgccagt cagttcgga aagtcgctc cgatttggt 
      caaaatcgg gtcttcggg acacacctc tggtgatga 
      attggaaac aaccacctc atcagatat tgaaattat 
      ttcaagtcc gaacggaga ttcgagtag tacgcaaag 
      taggcaaag cccagccaa gcaaacttg cacgaaaaa 
      taaatcaga cggcaccaa agtcgatct tttgtgcaa 
      aatgacgat gaaaaaagc caaaggcca cactagaga 
      attgtcgga aacttcgag cgtgagcga tgattcgca 
      ttcatccga agttgatcg gcttatcaa aaggattgc 
      ggaaatgac cgaaatttt ggagtccag agaatcgaa 
      attaattga tatcaatag gagaaatgc cgaattgca 
      agagttgaa caccaactt gcgacatca gctcgattc 
      gatgcttac caagcaatt ggaaactgc agatttgtc 
      tcaagtgac tgaagaata tacccagcg tttgtccgc 
      cttagagaa aaagtttca acaagcaat tcgggaaaa 
      agacacgct gaggaaaca actggaaca gagcaaaca 
      ggaggctgc cactaggct cagcaaaag cgatttgga 
      ctcacttat aagcgaaaa agatgaaat tattaagga 
      gttgcgtga ggaggggga aaagttgtc caaacagca 
      gttgcaaca ttcgaatat tatcaagaa gttgagggc 
      gaaagaaaa ggaaaatga gtcaacaat caaacattt 
      aaaagaaac aattgagga cttgtcgtc tgaagctga 
      ccgtttaaa gcgttcact aaccgcaaa agaggaagt 
      tgaaaggtc gcaaatcga agcagttca ccagctaac 
      agcaaaaaa taaaaaact agaaactga agttgataa 
      attccgaag tcagctcga cgacctcac acaaaagta 
      tgacaccgt gaagaagtc tttagacgc tgcgaaaaa 
      agaactcgt cgacaagaa caagactag ttctgagtt 
      aatcgcacg cgagcataa attggagag tttagagaa 
      cgaaaaaaa gcaaacgga gtcacaaaa cgcagcgat 
      tcttaacga actggagga actacggtc aaaaatgcg 
      acaactcga tttagacta cgcgaaaaa agaacagtc 
      gctacgtaa agaaaacaa cgatctttt gaggcgttt 
      ggaggacgc tgaggccag aaacgaaga attatcaca 
      atccgttct tgaagttag taagccttt ggtgagaca 
      attggagtc gttacaagc aacacatac gatgaaaat 
      cgccagttt tgaaagaat tgaacaaga aatgacgct 
      taaaataaa tgagttgca aactagatt acagacgtc 
      ccttaactc agaacggac ggtcaaaga cgagtctgt 
      gactctaaa aactagact ttcagattt ggaatcgga 
      actaagttc gcttaagca ccaaaacga gctgcttcg 
      agtggaagt ggagcagaa taaaaccga aaaacaaat 
      ctccgaaca ggagttagg tcgtgaaat taacgaatt 
      gaaggaaca gttagtgtc tgagagaca caaagtggc 
      cgagttggc gcaaaacgc gtccagttt gcaagaaca 
      gctggagaa atcagcgag tgaatccga gcgcaagaa 
      aagcactga agatgcgcc aaatttggg ccgaaactc 
      gccgcgcac tgcgagcaa ttcgccaac tttaagtct 
      ggggaaaat ttccgtggc ggaatcgct tggaagctc 
      gttttggtc gcaggatga gccctttga cgggggaca 
      accgccaag gtacaccaa catgttcga gatgcaaat 
      gcttcaaac caacttgaa acagcgcga cggtgaatt 
      gcaacaatt gcagtggga gttgaaccg gagggaaca 
      agagagggc gctcttgaa taaagaaat ctcgtcgct 
      tttgacccg tgttgagga acttgaaac caaagtcca 
      ggaatacga tgttttaaa aggacaaca tagtgagct 
      acagcaaca gtatgatac tctttgcca gttgtttgg 
      cgagaaagt tgaagaaaa cgaagaact gaagcttga 
      tttgcaaga cgtgaaaga gatgtacaa aagtcagat 
      tgatgagtt gttgaaaca gcaaaaaca gaacttgta 
      aagttatta ttatccagc gtgtacaga gatgctttt 
      atatgaatt tttattgat tagataaga gattaaagt 
      gtttgttgt tctattgcg gtttttatc atatgggaa 
      tattacgtt atcagttaa cacatgctg atatttggt 
      gttttgtca tggtaagga atatacttc acttaaaat 
      ttaacctgt gatataaat tatagcctc ttcaaatta 
      ttgtacaaa attccaact gaaataaat gttgtgttt 
      acgc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY