Status
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Gene information
General information
TC007707 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 43346..48956
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007707
    iBeetle screen iB_11052

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007707 RA
      MAKFADYFV ICGLDLSSG LEPDNISED NLHVSPLER 
      SYKGKVLAH YPENVSHNS FDASAVCMF CLPQGLKFR 
      TQKHSVTQA PVFHSFLIT REDGKRCYG FSLIFYEEV 
      RNRDICNAM QTLQAMYIT ELSSGLKVK AIQNTPQSR 
      SLPRHFKLS THNPSGAAL TYYDISKDK LFVSKSISL 
      ICQTAYVQA AKIFLENLF RCVPKRAVS SAGLSLESY 
      VFNLIYEVQ LPAPGKSLL ISLPPSDPH LPPVNAVLQ 
      SPAPPLELP HLDYSLRLL FIWLGVDIV IQIFTCLLL 
      ENQVLLRST DCQRLMVVA EGITSLLFP FTWPHVYVP 
      ILPASLHHF LDAPVPFVM GLYASSENI KVASEATLC 
      YVDIDKCKV QRPEESATF PHLEAFTDE IWAALDKYG 
      VHVPNSENS RSQEIMSRS CTLPGRPQN RRKLSIHDT 
      LDGDRPPSP PGSARSEAL QRIADIVRR TGVALDQNE 
      ATQPTDSYI EDLRFNNSI REIFLNRFV HIFQSYENF 
      VIFPNQDKD DWFNNRDSM QNFDKASFL SDQPEQHRH 
      FLWRFLESQ MFATLIDNK ILSTWGELD NNLIIFDNR 
      IKLLKQRYG GENLIRSLC YEPCTSAHD TQKLLERRL 
      TNPDFEAPP PREIMNVKS NPSRYFPLL DKEVLNKTP 
      VVNKGSIPR ASALRKGLS FTRNLPAEK TKNHQDMSP 
      ALIAQANWT FVEKLLKDC KAKTKRMLL AKLGAEGVT 
      LSSNNAADN FGAVEENTL VASLCEFLE RVWSHGLQR 
      KKGKSALWS HLTIYQETQ HQNRNKYLS ESRNIKGDL 
      PTLPDSLLF DIANVQAMT DVKTGIGMA KAWVRLALE 
      KKQLSKHLR TLLSDQTIL RSLYKRSAF LRCEEEKEQ 
      FLYHLLSLN AVDYFCFTS TYATTVIPY RIVIIPNKK 
      GSTTSANAW VVLSGTLSE TRRIPVPKS NNFEYKHHN 
      LGVLTTLRI GHDNSGLYA KWMIDYVLV RNEITGHTY 
      KFPCGRWLG RGVDDGSTE RLLVGNLMA LKVEGEETN 
      QSGSMGRTP PRCPSPAGL PRTELKQSQ IQHMLGDCV 
      NNIVKWHYR RNSERNTTL TALLCGENG LVHCLENVF 
      FMGFKSARL FVGKNHLWD YFVRVKEQF ESDLLEESS 
      GNRTASLER NHQETVAVW RCYCHLIDE IMCTSKALG 
      KDGKFQLFI CLSVREHLL HRMLGPMAL CKVTQEMYE 
      ESSFLRNRG LLTFLRQIL LPLDELDVV LENSVTHGI 
      SSPSSHV
    CDS sequence
    • >TC007707 RA
      atggcaaaa tttgctgat tatttcgta atttgtggc 
      ttagacttg agttcaggt ctcgaacca gacaatata 
      tcagaagac aacttgcat gtctctccg ttggaacgt 
      tcatacaaa ggcaaagtt ttggcacac tatcccgaa 
      aacgtgtct cataattcc tttgacgct tctgcagtt 
      tgcatgttc tgtttgccc caaggatta aaattccgg 
      actcaaaaa cactctgtc actcaagcc ccagttttc 
      cactcattt cttataact cgagaagac ggcaagcgg 
      tgctacggc tttagcttg attttctac gaagaagtc 
      cgtaataga gacatttgc aacgccatg caaacgctt 
      caagctatg tacatcacg gaattatca agtggatta 
      aaagtcaaa gcgatccaa aacacacct caatcccga 
      tctctacca cgtcatttc aaactcagt acacataat 
      cccagtggt gcagctctc acctattac gatatttct 
      aaagacaag ctttttgta tcaaaaagt atcagtctg 
      atatgccag acagcttac gtacaagct gctaaaatt 
      tttctcgag aatttattc cgttgtgtg ccgaaacgt 
      gcggtatct tcggcaggt ttaagcctt gaaagttac 
      gtatttaat ttaatttat gaagtacaa ttgccggca 
      cccggaaaa agtctttta atctcattg cctccaagt 
      gacccacat ttacctcca gttaacgca gttttacaa 
      agtccagct cctccactt gaactgccc catctcgat 
      tattctcta agattgttg tttatttgg cttggagtt 
      gatatcgtt atacaaatc ttcacttgt ctcctattg 
      gaaaatcaa gtcttgtta cgtagcacc gattgccaa 
      cgtttaatg gtagtggct gaaggtatc acttcgttg 
      ttatttcct ttcacatgg ccacatgtt tacgttcct 
      atactacct gcctccttg caccatttt ttggatgct 
      cctgttcca tttgtaatg gggctttac gcctcttct 
      gaaaacatc aaggtggcg tcggaagca actctgtgt 
      tacgttgat attgataag tgtaaagta caaagaccg 
      gaggaaagt gcaacattt ccccatttg gaagctttc 
      accgacgaa atatgggca gctttggac aaatatgga 
      gtccatgtt cctaattct gagaatagt cgtagtcag 
      gaaatcatg tcgcggagt tgtaccctg ccggggcgc 
      ccccaaaat cggcgcaaa ctctcaatt catgacact 
      ctcgatggg gacagaccc ccatctccc ccaggttca 
      gctcgatct gaggcttta caacgaatc gctgatata 
      gttagaaga acaggcgtg gccttggac cagaacgaa 
      gccactcaa cctacagat tcctacatt gaggattta 
      cgttttaat aattcaatt cgagagatt ttcctcaat 
      cgtttcgtt cacattttc caatcgtac gaaaatttc 
      gttatattt cccaatcag gataaggac gattggttc 
      aataatcgt gattcaatg caaaatttc gataaggcg 
      tcttttttg tccgatcaa ccggaacaa catcgccac 
      tttctgtgg aggtttctc gaatcgcaa atgtttgcc 
      acacttatc gataataaa attttatcc acatggggc 
      gagcttgat aataattta ataattttc gataatcga 
      attaaatta ttgaaacaa cgctacggt ggtgagaat 
      ttaatcagg tctttgtgt tacgaaccg tgtacgagt 
      gcacacgat acacagaaa cttctagaa cgaagattg 
      acgaatcca gattttgaa gctccacca ccaagagaa 
      ataatgaac gtcaagtca aatccaagt cggtatttt 
      ccccttttg gataaggaa gttttaaac aagacacct 
      gtcgttaat aaaggaagt atacctaga gcgagtgca 
      ctgcgtaaa ggtttgtca tttacgcgt aatttacct 
      gctgagaaa acgaaaaat catcaagac atgtcgccg 
      gctttaata gcacaagca aattggaca tttgtcgaa 
      aaattactc aaagattgt aaagccaaa actaaaaga 
      atgctattg gcaaagttg ggagccgaa ggtgtaaca 
      ctttcaagt aacaatgct gctgataat ttcggagca 
      gttgaagaa aatactttg gtggcttca ttgtgtgag 
      tttttggaa cgagtttgg tcgcatgga cttcagcgg 
      aaaaagggc aaatcggct ttgtggtca cacttgacc 
      atatatcag gagacacaa caccaaaac aggaataaa 
      tatctgagt gagagtaga aacattaag ggggattta 
      ccaacactt cccgattcg cttttattt gatatcgcg 
      aacgtgcaa gcgatgacc gacgtgaaa accggtatc 
      ggaatggcc aaagcctgg gtgcgttta gccctcgag 
      aaaaaacaa ctatcaaaa cacctccgc accctcctt 
      tcggaccaa accatcttg cgcagtctt tacaaacgt 
      tcggctttt ctccgttgt gaagaagaa aaagaacaa 
      ttcctctac catctctta tcactaaac gccgtcgat 
      tatttctgt tttaccagt acatatgcc accactgtg 
      ataccctac cgcattgtg ataattcca aacaaaaag 
      ggttcaacc acctcggca aatgcctgg gttgtactc 
      tcgggtact ctttcggaa actcggcgc attcctgta 
      cctaaatca aacaatttt gaatacaaa catcacaat 
      ttgggcgta ttgacaaca ctgaggata ggacatgac 
      aattcgggg ttgtacgca aaatggatg attgactat 
      gttttagta cgaaacgaa atcacgggt cacacttac 
      aaatttcct tgtggaaga tggttaggt cgaggagtt 
      gatgatgga tcaactgaa cgacttttg gtgggtaat 
      ttaatggcg ttgaaagtc gaaggcgaa gaaacgaac 
      cagagtgga agtatggga cgaacacct ccaaggtgt 
      ccgtcccca gcgggactt ccccgaacc gaattaaaa 
      caatcacaa attcagcac atgttaggt gactgtgtt 
      aataatatc gtcaaatgg cactatagg agaaactct 
      gagcgaaat acaacattg acggctcta ttatgtggc 
      gaaaatggt ttggtccat tgtttggaa aatgttttt 
      ttcatgggg ttcaagtcg gcgagatta ttcgttggg 
      aaaaatcat ctctgggat tatttcgta cgtgtaaag 
      gaacaattt gagtcagat ttactagag gaaagttca 
      ggtaatcga acggcgagt ttagaacgt aatcatcaa 
      gagaccgtc gctgtttgg agatgctat tgtcattta 
      attgacgaa attatgtgt acgagtaaa gcgttgggg 
      aaagacgga aaatttcag ttgtttatt tgtctgagt 
      gtcagggag cacttgctg catcggatg ttgggacca 
      atggcgtta tgtaaagtg acacaagaa atgtatgaa 
      gaaagttcg tttttgaga aatcgtggt ttattaacg 
      tttctcaga caaatttta ttgccattg gacgaactt 
      gatgttgtt cttgaaaat tcagtaact catggaatt 
      tcatcacct tcatctcat gtctaa
    mRNA sequence
    • >TC007707 RA
      ttaaagaaa aatgttttt ttgaataaa acaacaata 
      cttgtgttg caaaatcat gtattgttt gattctagc 
      tttgataaa tatgggctt ggatcaaaa tttgtataa 
      ttggttttg ttggtgtag atccaaagt tccagtcgg 
      tgtaatgtt caagatggc tgaaaaacg aaaagaaaa 
      tagagcgta aatttgtaa catattttt tattaaaga 
      cgcataaag tacaaaccg ataatatta agcaacaat 
      gcattgtga taaaaccat ctagaatca gctctctgc 
      gagacaaat cgtcttaag cttaggtta agtgaaaat 
      ggcaaaatt tgctgatta tttcgtaat ttgtggctt 
      agacttgag ttcaggtct cgaaccaga caatatatc 
      agaagacaa cttgcatgt ctctccgtt ggaacgttc 
      atacaaagg caaagtttt ggcacacta tcccgaaaa 
      cgtgtctca taattcctt tgacgcttc tgcagtttg 
      catgttctg tttgcccca aggattaaa attccggac 
      tcaaaaaca ctctgtcac tcaagcccc agttttcca 
      ctcatttct tataactcg agaagacgg caagcggtg 
      ctacggctt tagcttgat tttctacga agaagtccg 
      taatagaga catttgcaa cgccatgca aacgcttca 
      agctatgta catcacgga attatcaag tggattaaa 
      agtcaaagc gatccaaaa cacacctca atcccgatc 
      tctaccacg tcatttcaa actcagtac acataatcc 
      cagtggtgc agctctcac ctattacga tatttctaa 
      agacaagct ttttgtatc aaaaagtat cagtctgat 
      atgccagac agcttacgt acaagctgc taaaatttt 
      tctcgagaa tttattccg ttgtgtgcc gaaacgtgc 
      ggtatcttc ggcaggttt aagccttga aagttacgt 
      atttaattt aatttatga agtacaatt gccggcacc 
      cggaaaaag tcttttaat ctcattgcc tccaagtga 
      cccacattt acctccagt taacgcagt tttacaaag 
      tccagctcc tccacttga actgcccca tctcgatta 
      ttctctaag attgttgtt tatttggct tggagttga 
      tatcgttat acaaatctt cacttgtct cctattgga 
      aaatcaagt cttgttacg tagcaccga ttgccaacg 
      tttaatggt agtggctga aggtatcac ttcgttgtt 
      atttccttt cacatggcc acatgttta cgttcctat 
      actacctgc ctccttgca ccatttttt ggatgctcc 
      tgttccatt tgtaatggg gctttacgc ctcttctga 
      aaacatcaa ggtggcgtc ggaagcaac tctgtgtta 
      cgttgatat tgataagtg taaagtaca aagaccgga 
      ggaaagtgc aacatttcc ccatttgga agctttcac 
      cgacgaaat atgggcagc tttggacaa atatggagt 
      ccatgttcc taattctga gaatagtcg tagtcagga 
      aatcatgtc gcggagttg taccctgcc ggggcgccc 
      ccaaaatcg gcgcaaact ctcaattca tgacactct 
      cgatgggga cagaccccc atctccccc aggttcagc 
      tcgatctga ggctttaca acgaatcgc tgatatagt 
      tagaagaac aggcgtggc cttggacca gaacgaagc 
      cactcaacc tacagattc ctacattga ggatttacg 
      ttttaataa ttcaattcg agagatttt cctcaatcg 
      tttcgttca cattttcca atcgtacga aaatttcgt 
      tatatttcc caatcagga taaggacga ttggttcaa 
      taatcgtga ttcaatgca aaatttcga taaggcgtc 
      ttttttgtc cgatcaacc ggaacaaca tcgccactt 
      tctgtggag gtttctcga atcgcaaat gtttgccac 
      acttatcga taataaaat tttatccac atggggcga 
      gcttgataa taatttaat aattttcga taatcgaat 
      taaattatt gaaacaacg ctacggtgg tgagaattt 
      aatcaggtc tttgtgtta cgaaccgtg tacgagtgc 
      acacgatac acagaaact tctagaacg aagattgac 
      gaatccaga ttttgaagc tccaccacc aagagaaat 
      aatgaacgt caagtcaaa tccaagtcg gtattttcc 
      ccttttgga taaggaagt tttaaacaa gacacctgt 
      cgttaataa aggaagtat acctagagc gagtgcact 
      gcgtaaagg tttgtcatt tacgcgtaa tttacctgc 
      tgagaaaac gaaaaatca tcaagacat gtcgccggc 
      tttaatagc acaagcaaa ttggacatt tgtcgaaaa 
      attactcaa agattgtaa agccaaaac taaaagaat 
      gctattggc aaagttggg agccgaagg tgtaacact 
      ttcaagtaa caatgctgc tgataattt cggagcagt 
      tgaagaaaa tactttggt ggcttcatt gtgtgagtt 
      tttggaacg agtttggtc gcatggact tcagcggaa 
      aaagggcaa atcggcttt gtggtcaca cttgaccat 
      atatcagga gacacaaca ccaaaacag gaataaata 
      tctgagtga gagtagaaa cattaaggg ggatttacc 
      aacacttcc cgattcgct tttatttga tatcgcgaa 
      cgtgcaagc gatgaccga cgtgaaaac cggtatcgg 
      aatggccaa agcctgggt gcgtttagc cctcgagaa 
      aaaacaact atcaaaaca cctccgcac cctcctttc 
      ggaccaaac catcttgcg cagtcttta caaacgttc 
      ggcttttct ccgttgtga agaagaaaa agaacaatt 
      cctctacca tctcttatc actaaacgc cgtcgatta 
      tttctgttt taccagtac atatgccac cactgtgat 
      accctaccg cattgtgat aattccaaa caaaaaggg 
      ttcaaccac ctcggcaaa tgcctgggt tgtactctc 
      gggtactct ttcggaaac tcggcgcat tcctgtacc 
      taaatcaaa caattttga atacaaaca tcacaattt 
      gggcgtatt gacaacact gaggatagg acatgacaa 
      ttcggggtt gtacgcaaa atggatgat tgactatgt 
      tttagtacg aaacgaaat cacgggtca cacttacaa 
      atttccttg tggaagatg gttaggtcg aggagttga 
      tgatggatc aactgaacg acttttggt gggtaattt 
      aatggcgtt gaaagtcga aggcgaaga aacgaacca 
      gagtggaag tatgggacg aacacctcc aaggtgtcc 
      gtccccagc gggacttcc ccgaaccga attaaaaca 
      atcacaaat tcagcacat gttaggtga ctgtgttaa 
      taatatcgt caaatggca ctataggag aaactctga 
      gcgaaatac aacattgac ggctctatt atgtggcga 
      aaatggttt ggtccattg tttggaaaa tgttttttt 
      catggggtt caagtcggc gagattatt cgttgggaa 
      aaatcatct ctgggatta tttcgtacg tgtaaagga 
      acaatttga gtcagattt actagagga aagttcagg 
      taatcgaac ggcgagttt agaacgtaa tcatcaaga 
      gaccgtcgc tgtttggag atgctattg tcatttaat 
      tgacgaaat tatgtgtac gagtaaagc gttggggaa 
      agacggaaa atttcagtt gtttatttg tctgagtgt 
      cagggagca cttgctgca tcggatgtt gggaccaat 
      ggcgttatg taaagtgac acaagaaat gtatgaaga 
      aagttcgtt tttgagaaa tcgtggttt attaacgtt 
      tctcagaca aattttatt gccattgga cgaacttga 
      tgttgttct tgaaaattc agtaactca tggaatttc 
      atcaccttc atctcatgt ctaattttt tttgtctag 
      tcctttttt ttaccaccg ttgatatta tgttaattt 
      tccagagtg atattgtat atatttttc taagtttgt 
      atatattgt ttaaagtgg cctaaatca tttacaaag 
      aacaagttt aattcctat aaccttgcc aaagttaaa 
      tctagtaag cagaattta tttttattt tacataatt 
      tgcatatca gtaatttta cgtgactgg ggcagctgt 
      cttcctttt ctttttttg ttggtctca gtgtttgag 
      aaattaaat tttgtgagt tcattgtta tgtgatatt 
      gtaagaaaa ttgtgaaat tattttgat gttgacaca 
      attctttgt gtgtaggat ttggaagcc aaagtagtt 
      ttgcaactt atagactcg taaaaagat ccgttagat 
      gagattgta attaattga aaagttgta ataataaat 
      tccatgtaa actt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY