Status
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Gene information
General information
TC031720
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004071 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451702.1: 162481..171115
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC031720
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_11051

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031720 RA
      MSENVHRMA DGTMAPVNY DANVDYVIT EYMERLGTR 
LNILETELK YAWRALDLL SQEYIKMWE RLEKLEGLL 
YEQQSVISQ LLDFYTTGG CRNMGAGAT AGELEVIRE 
ILGNGTVED GLEEGQDVS RTQELELEE LHVPDEAFY 
RSLNQAYRE DLGGQGSQL GMIWEEKEE TDDNNEKKN 
DCCLVEGEE VFSALDYKD YRGNSPCVS EQDIAQLSR 
IDSIDHVAI EKLNELDRL SSKLQQDSD NIKQLQRKL 
LESPLSMSQ DDEPKDDPN WSFSNSNQT DLLILKTEL 
PNFAPRPSS RLSVTDSVI ATDNEVAEV LAGGLRGST 
PTSPRRVAS CTAPFTTVT GRLAYSAAV QNIDSKNPF 
YSSLVAASY ETIPCSTPS IFNARAETA PSPSLSIRT 
RQDGYIATN DQKGSPSPP PPAPEPNPV FLATEPAPQ 
QSIKSPKSS PKRKNVVKS DSGLSSMSG WSSLEKSPG 
SPKINKGHH HDNTILPGG HHLSAFTTV KSPCSLETS 
EGYGTFVPA PAPASDINV SPRRKKHTK ANSFTWNDQ 
PAIYSVAGS NRETYTSVY TSNSADYIT QTINYPDLV 
EPYENNELQ RTTSGTTER RRSLSRMFS TSNGDGVAN 
HEGYKTAMQ RTMFPTGNI TDALSYYPT STRYDEYNE 
AWLNPQEQQ ESPSSSMRS TEPSEYNQS PYLDQRRIY 
ENQDRWNQR QEIPYLELD LRNRNVPEY VVQQNENKF 
FDPSSVIIT QSGYITINS DTIDSSREE KKKFKRGNT 
LKSAMSSVS HWLPDLHLT KRHRSYSLP SGVRREDLS 
KPKSNVGGR KKKKNAIVS TVSGILQKA KRKSYFHHH 
TQSLSDPEQ SENEWVLQR ASSVVSEDD RSEDSYSMF 
EGQAERDLF AKVAKPKPE DLQVVKVSP PPLNDHVEE 
EVSGGSASS SIFPTVGEP SKKSSESEK SDETVITES 
KMVVVVGGA SMEFAVSRA LGKYRQQKQ TSLPDDLSL 
EEPQGVVIE EIVEEEPKV DKLVEQIST ESEVSHTEP 
VLRAQNRYL PKHQQSLEI PGHKEDDDS RSTHSWRST 
SRVSSRRQS TEDSIDSED EWYCYELRK LEEMERQSQ 
LEAEMGETL REEPELEEE YEPFEEVKE RMSFVLREL 
RMKAKVVEG VLEEGDNNL HTLQEAKNR ERKSIHLEE 
AHMLFDKIA QNIKEEEEE QAKDEEHSS GETSGPDSP 
QQSENEEEN TEEFARCHV TPREEEQAE EASAVPEIQ 
VEPEVAKPE TKETAGSKW KLLKALKER KAEEKSNQD 
KVEPASEKD KVSMLGMVQ KFYEIHVKT
    CDS sequence
    • >TC031720 RA
      atgagcgaa aacgtgcac cggatggcc gacggtacc 
atggcaccc gtcaactac gacgccaac gttgactat 
gtcatcacc gaatacatg gaacggctc ggaaccaga 
ctcaacatc cttgaaacg gagctgaaa tacgcctgg 
cgtgccctt gatttactc tcacaggag tatatcaaa 
atgtgggag aggttggag aagttggag gggttgttg 
tacgagcaa cagagtgtc atttcccaa ttgttggat 
ttttacacc acggggggc tgtcgcaac atgggggcg 
ggagcaaca gctggggaa ctggaagtg attcgtgag 
attctgggc aatgggact gtggaggac gggctggag 
gaggggcaa gacgtgtca aggacacaa gagttggag 
ttggaggag ttgcatgtg ccggatgag gcgttctat 
cgcagcttg aaccaggcg tatcgggag gacttgggg 
gggcaaggg tcacagttg gggatgatt tgggaggag 
aaggaggaa actgacgac aacaatgag aagaaaaat 
gattgttgt ttggttgaa ggcgaggag gtttttagt 
gctttggac tacaaggac tatcggggg aattccccg 
tgtgttagt gaacaggat atagctcaa ttgtcacgc 
attgactcc atagatcac gtcgccatt gaaaaatta 
aacgaactg gaccgtttg agcagtaaa ttgcaacaa 
gactcagat aatattaaa caacttcaa cgtaaactc 
ctcgaatct cctctttcc atgtcgcaa gacgacgaa 
ccaaaagac gatcctaac tggagtttc tccaattcc 
aaccaaacc gacctcctc atcctcaaa accgaactt 
ccaaacttc gccccacgt cctagttcc cgcctctcc 
gtcactgat agtgtcatt gccactgat aacgaagtc 
gccgaagtg ctcgccggt ggtttacgc gggagcacc 
cccacgtcc ccccggcgc gtcgcctcc tgcaccgcc 
cccttcacc accgtcacc ggccgcctc gcctacagc 
gccgctgtc caaaacatc gactccaaa aacccgttt 
tacagtagt ctagtggcc gcctcctac gagaccatc 
ccctgctcc accccttcg atcttcaac gcgcgcgcc 
gaaaccgcc ccctcgccc tccctctcc atccgcacg 
cgccaagac ggctacatt gcaacgaac gaccagaaa 
ggttcaccg agtccgccc ccaccggcc cccgagccc 
aacccagtc ttcctagcc actgagccg gcgccacag 
cagtcaatt aagtcgccc aaaagctcc cccaagcgc 
aaaaacgtc gtcaaatcc gactcaggg ctgtcgtca 
atgagtggg tggtcaagt ctcgagaaa tcaccagga 
agtccgaaa atcaacaaa gggcaccac catgacaac 
accatccta cctggtggc caccacctt tcggcgttc 
acgacggtg aagtcaccg tgtagtttg gagacgagc 
gaagggtac ggtactttt gtcccagca ccggcccca 
gcctccgat attaatgtg agtccaagg aggaaaaaa 
cacaccaag gcgaattct ttcacttgg aacgaccaa 
ccagcgatt tactccgtg gcggggagc aaccgcgag 
acttacact tctgtctat actagtaac agtgccgat 
tatatcacc cagactatc aattacccg gatttggtc 
gagccttac gaaaacaac gaattgcaa cgaactact 
tcaggtacc accgagagg aggaggtca ctctcgagg 
atgttctcc acgagtaac ggcgacggg gtcgctaac 
catgaaggt tacaagacg gcaatgcaa cgcacgatg 
ttccccacc ggtaacatc accgacgcc ttgtcgtac 
tacccgacc tccaccaga tacgacgag tacaacgag 
gcctggctt aacccccaa gaacaacaa gaaagtccg 
agcagttcc atgcgttcc acggaaccc tcggagtac 
aaccaaagc ccctacctg gaccagagg agaatctac 
gagaaccag gacaggtgg aaccaacgg caggagatc 
ccttacttg gagctcgac ctccggaac cggaacgtc 
cctgaatat gtggtccaa caaaacgag aacaagttc 
tttgacccc agctcggtc ataatcact cagtcgggc 
tatattacc atcaattcg gacacaatc gactcatcc 
cgggaggaa aaaaagaaa ttcaagcgg ggtaatacg 
ttaaaatcg gccatgagt tcggtaagt cattggtta 
ccagacctg cacctcacc aagcgacac cgctcgtat 
tccttaccg tcgggggtc aggcgggag gatttgtcc 
aaacctaaa agtaacgtc ggcggtcgc aagaagaag 
aaaaacgcg attgtgtcc acggtttcg gggattttg 
caaaaagcc aagcggaaa agctacttc caccaccac 
acccagtca ctctctgac cccgaacag tccgagaac 
gagtgggtg ttacaacgg gccagttct gtggtctct 
gaagatgat agaagtgag gatagttac agtatgttt 
gaaggacaa gctgagcga gacctcttc gccaaagtg 
gcgaaaccc aaacccgag gacctccaa gtggttaaa 
gtgtctcca ccaccactc aacgaccac gtggaggag 
gaagtctcc ggcgggagc gcctccagt agcattttc 
cccacggtc ggcgaaccg tcgaagaag agttccgag 
tcggagaaa agtgacgaa acggtcatc accgaaagc 
aagatggtc gtggtggtt ggaggcgcc tccatggag 
ttcgccgtg tcccgagcc ttgggcaaa taccgccaa 
cagaagcaa acctctctc cccgacgat ctctccttg 
gaggaaccc caaggggtc gtgatcgag gaaatcgtc 
gaagaggaa ccaaaagtg gacaagttg gtcgaacaa 
atcagtact gaaagcgaa gtcagtcac acggaaccg 
gtcctaagg gcccaaaat cgctacttg cccaaacac 
caacaaagt ttggaaata ccaggacat aaggaggac 
gatgacagt cggagcacg cactcctgg cggagcacc 
tcccgagtg tcctcgcgc cgccaaagc accgaagac 
agcatcgac agtgaggac gagtggtat tgctacgag 
ttgcggaag ctggaggag atggaacgt cagtcgcag 
cttgaagcc gagatgggt gaaaccctc cgggaggag 
cccgagttg gaggaggag tacgaaccg ttcgaggag 
gtcaaggag cggatgtcc ttcgtgttg cgagagcta 
cgcatgaag gccaaggtc gtggagggg gttctggag 
gagggcgac aacaacctc cacaccctc caagaggcg 
aagaaccgc gaacgcaaa tccatccat ttggaggag 
gcgcacatg ttgttcgac aaaatcgcc caaaacatc 
aaggaggag gaggaggag caagccaag gacgaggaa 
cactcctcg ggggagacc tccgggccg gactccccc 
cagcagagc gagaacgag gaggagaac acggaggag 
tttgccagg tgtcacgtc accccccgt gaggaggag 
caagctgag gaggcttcc gccgtcccc gaaatccag 
gtcgaaccg gaagtggcg aagcccgag accaaggaa 
accgccggg agtaagtgg aagctcttg aaggctttg 
aaggagcgg aaggcggag gagaagagc aaccaggac 
aaggttgag ccagctagt gagaaggac aaagtgagt 
atgctgggg atggtgcaa aagttctac gaaatccac 
gtcaaaacc tag
    mRNA sequence
    • >TC031720 RA
      catgtgaga tcgaaacga accgtaaag ctgaccggt 
ctcccctgt taagaagcg tcttaacac ggtggacct 
tttactttt tttattttg taaattttg gccactaat 
tgctgattt tataggact tggggggcg tttaggagc 
gtaatagat gttgaagca tgccttctt ccacacgga 
cgaaacaat gactttagt gttgccagt gtctcgtga 
tgttcgcga gcacgccaa tccgcacca tgccgcgaa 
aaaagacta aaatgtagt gtaaattct ggtgaaaaa 
tgagcgaaa acgtgcacc ggatggccg acggtacca 
tggcacccg tcaactacg acgccaacg ttgactatg 
tcatcaccg aatacatgg aacggctcg gaaccagac 
tcaacatcc ttgaaacgg agctgaaat acgcctggc 
gtgcccttg atttactct cacaggagt atatcaaaa 
tgtgggaga ggttggaga agttggagg ggttgttgt 
acgagcaac agagtgtca tttcccaat tgttggatt 
tttacacca cggggggct gtcgcaaca tgggggcgg 
gagcaacag ctggggaac tggaagtga ttcgtgaga 
ttctgggca atgggactg tggaggacg ggctggagg 
aggggcaag acgtgtcaa ggacacaag agttggagt 
tggaggagt tgcatgtgc cggatgagg cgttctatc 
gcagcttga accaggcgt atcgggagg acttggggg 
ggcaagggt cacagttgg ggatgattt gggaggaga 
aggaggaaa ctgacgaca acaatgaga agaaaaatg 
attgttgtt tggttgaag gcgaggagg tttttagtg 
ctttggact acaaggact atcggggga attccccgt 
gtgttagtg aacaggata tagctcaat tgtcacgca 
ttgactcca tagatcacg tcgccattg aaaaattaa 
acgaactgg accgtttga gcagtaaat tgcaacaag 
actcagata atattaaac aacttcaac gtaaactcc 
tcgaatctc ctctttcca tgtcgcaag acgacgaac 
caaaagacg atcctaact ggagtttct ccaattcca 
accaaaccg acctcctca tcctcaaaa ccgaacttc 
caaacttcg ccccacgtc ctagttccc gcctctccg 
tcactgata gtgtcattg ccactgata acgaagtcg 
ccgaagtgc tcgccggtg gtttacgcg ggagcaccc 
ccacgtccc cccggcgcg tcgcctcct gcaccgccc 
ccttcacca ccgtcaccg gccgcctcg cctacagcg 
ccgctgtcc aaaacatcg actccaaaa acccgtttt 
acagtagtc tagtggccg cctcctacg agaccatcc 
cctgctcca ccccttcga tcttcaacg cgcgcgccg 
aaaccgccc cctcgccct ccctctcca tccgcacgc 
gccaagacg gctacattg caacgaacg accagaaag 
gttcaccga gtccgcccc caccggccc ccgagccca 
acccagtct tcctagcca ctgagccgg cgccacagc 
agtcaatta agtcgccca aaagctccc ccaagcgca 
aaaacgtcg tcaaatccg actcagggc tgtcgtcaa 
tgagtgggt ggtcaagtc tcgagaaat caccaggaa 
gtccgaaaa tcaacaaag ggcaccacc atgacaaca 
ccatcctac ctggtggcc accaccttt cggcgttca 
cgacggtga agtcaccgt gtagtttgg agacgagcg 
aagggtacg gtacttttg tcccagcac cggccccag 
cctccgata ttaatgtga gtccaagga ggaaaaaac 
acaccaagg cgaattctt tcacttgga acgaccaac 
cagcgattt actccgtgg cggggagca accgcgaga 
cttacactt ctgtctata ctagtaaca gtgccgatt 
atatcaccc agactatca attacccgg atttggtcg 
agccttacg aaaacaacg aattgcaac gaactactt 
caggtacca ccgagagga ggaggtcac tctcgagga 
tgttctcca cgagtaacg gcgacgggg tcgctaacc 
atgaaggtt acaagacgg caatgcaac gcacgatgt 
tccccaccg gtaacatca ccgacgcct tgtcgtact 
acccgacct ccaccagat acgacgagt acaacgagg 
cctggctta acccccaag aacaacaag aaagtccga 
gcagttcca tgcgttcca cggaaccct cggagtaca 
accaaagcc cctacctgg accagagga gaatctacg 
agaaccagg acaggtgga accaacggc aggagatcc 
cttacttgg agctcgacc tccggaacc ggaacgtcc 
ctgaatatg tggtccaac aaaacgaga acaagttct 
ttgacccca gctcggtca taatcactc agtcgggct 
atattacca tcaattcgg acacaatcg actcatccc 
gggaggaaa aaaagaaat tcaagcggg gtaatacgt 
taaaatcgg ccatgagtt cggtaagtc attggttac 
cagacctgc acctcacca agcgacacc gctcgtatt 
ccttaccgt cgggggtca ggcgggagg atttgtcca 
aacctaaaa gtaacgtcg gcggtcgca agaagaaga 
aaaacgcga ttgtgtcca cggtttcgg ggattttgc 
aaaaagcca agcggaaaa gctacttcc accaccaca 
cccagtcac tctctgacc ccgaacagt ccgagaacg 
agtgggtgt tacaacggg ccagttctg tggtctctg 
aagatgata gaagtgagg atagttaca gtatgtttg 
aaggacaag ctgagcgag acctcttcg ccaaagtgg 
cgaaaccca aacccgagg acctccaag tggttaaag 
tgtctccac caccactca acgaccacg tggaggagg 
aagtctccg gcgggagcg cctccagta gcattttcc 
ccacggtcg gcgaaccgt cgaagaaga gttccgagt 
cggagaaaa gtgacgaaa cggtcatca ccgaaagca 
agatggtcg tggtggttg gaggcgcct ccatggagt 
tcgccgtgt cccgagcct tgggcaaat accgccaac 
agaagcaaa cctctctcc ccgacgatc tctccttgg 
aggaacccc aaggggtcg tgatcgagg aaatcgtcg 
aagaggaac caaaagtgg acaagttgg tcgaacaaa 
tcagtactg aaagcgaag tcagtcaca cggaaccgg 
tcctaaggg cccaaaatc gctacttgc ccaaacacc 
aacaaagtt tggaaatac caggacata aggaggacg 
atgacagtc ggagcacgc actcctggc ggagcacct 
cccgagtgt cctcgcgcc gccaaagca ccgaagaca 
gcatcgaca gtgaggacg agtggtatt gctacgagt 
tgcggaagc tggaggaga tggaacgtc agtcgcagc 
ttgaagccg agatgggtg aaaccctcc gggaggagc 
ccgagttgg aggaggagt acgaaccgt tcgaggagg 
tcaaggagc ggatgtcct tcgtgttgc gagagctac 
gcatgaagg ccaaggtcg tggaggggg ttctggagg 
agggcgaca acaacctcc acaccctcc aagaggcga 
agaaccgcg aacgcaaat ccatccatt tggaggagg 
cgcacatgt tgttcgaca aaatcgccc aaaacatca 
aggaggagg aggaggagc aagccaagg acgaggaac 
actcctcgg gggagacct ccgggccgg actcccccc 
agcagagcg agaacgagg aggagaaca cggaggagt 
ttgccaggt gtcacgtca ccccccgtg aggaggagc 
aagctgagg aggcttccg ccgtccccg aaatccagg 
tcgaaccgg aagtggcga agcccgaga ccaaggaaa 
ccgccggga gtaagtgga agctcttga aggctttga 
aggagcgga aggcggagg agaagagca accaggaca 
aggttgagc cagctagtg agaaggaca aagtgagta 
tgctgggga tggtgcaaa agttctacg aaatccacg 
tcaaaacct agcttagtc aagcgcttc gccttattg 
taaccggct tttagaggt caccacgag tcaattcga 
atccaggag tttgtgttt agtcatatc gcactcata 
tcaatgtaa ttatcatca cggagattg acattatgc 
agtgtttat actatcgtt gacaataac gcgagcgta 
cgattctaa accactcta ctgtacata acgaccaag 
acgtgatag tataacgcg ctgattttc ctactttag 
cacctctag actggccat aatgacaat ctcgactag 
atcggtgtt agcagtatt atgcttgac ttagggaca 
ttgtgctaa gatttgttg atttgaata aaatctgtt 
acccctt
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