Status
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Gene information
General information
TC031720
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004071
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451702.1: 162481..171115
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031720
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_11051

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031720 RA
      MSENVHRMA DGTMAPVNY DANVDYVIT EYMERLGTR 
      LNILETELK YAWRALDLL SQEYIKMWE RLEKLEGLL 
      YEQQSVISQ LLDFYTTGG CRNMGAGAT AGELEVIRE 
      ILGNGTVED GLEEGQDVS RTQELELEE LHVPDEAFY 
      RSLNQAYRE DLGGQGSQL GMIWEEKEE TDDNNEKKN 
      DCCLVEGEE VFSALDYKD YRGNSPCVS EQDIAQLSR 
      IDSIDHVAI EKLNELDRL SSKLQQDSD NIKQLQRKL 
      LESPLSMSQ DDEPKDDPN WSFSNSNQT DLLILKTEL 
      PNFAPRPSS RLSVTDSVI ATDNEVAEV LAGGLRGST 
      PTSPRRVAS CTAPFTTVT GRLAYSAAV QNIDSKNPF 
      YSSLVAASY ETIPCSTPS IFNARAETA PSPSLSIRT 
      RQDGYIATN DQKGSPSPP PPAPEPNPV FLATEPAPQ 
      QSIKSPKSS PKRKNVVKS DSGLSSMSG WSSLEKSPG 
      SPKINKGHH HDNTILPGG HHLSAFTTV KSPCSLETS 
      EGYGTFVPA PAPASDINV SPRRKKHTK ANSFTWNDQ 
      PAIYSVAGS NRETYTSVY TSNSADYIT QTINYPDLV 
      EPYENNELQ RTTSGTTER RRSLSRMFS TSNGDGVAN 
      HEGYKTAMQ RTMFPTGNI TDALSYYPT STRYDEYNE 
      AWLNPQEQQ ESPSSSMRS TEPSEYNQS PYLDQRRIY 
      ENQDRWNQR QEIPYLELD LRNRNVPEY VVQQNENKF 
      FDPSSVIIT QSGYITINS DTIDSSREE KKKFKRGNT 
      LKSAMSSVS HWLPDLHLT KRHRSYSLP SGVRREDLS 
      KPKSNVGGR KKKKNAIVS TVSGILQKA KRKSYFHHH 
      TQSLSDPEQ SENEWVLQR ASSVVSEDD RSEDSYSMF 
      EGQAERDLF AKVAKPKPE DLQVVKVSP PPLNDHVEE 
      EVSGGSASS SIFPTVGEP SKKSSESEK SDETVITES 
      KMVVVVGGA SMEFAVSRA LGKYRQQKQ TSLPDDLSL 
      EEPQGVVIE EIVEEEPKV DKLVEQIST ESEVSHTEP 
      VLRAQNRYL PKHQQSLEI PGHKEDDDS RSTHSWRST 
      SRVSSRRQS TEDSIDSED EWYCYELRK LEEMERQSQ 
      LEAEMGETL REEPELEEE YEPFEEVKE RMSFVLREL 
      RMKAKVVEG VLEEGDNNL HTLQEAKNR ERKSIHLEE 
      AHMLFDKIA QNIKEEEEE QAKDEEHSS GETSGPDSP 
      QQSENEEEN TEEFARCHV TPREEEQAE EASAVPEIQ 
      VEPEVAKPE TKETAGSKW KLLKALKER KAEEKSNQD 
      KVEPASEKD KVSMLGMVQ KFYEIHVKT
    CDS sequence
    • >TC031720 RA
      atgagcgaa aacgtgcac cggatggcc gacggtacc 
      atggcaccc gtcaactac gacgccaac gttgactat 
      gtcatcacc gaatacatg gaacggctc ggaaccaga 
      ctcaacatc cttgaaacg gagctgaaa tacgcctgg 
      cgtgccctt gatttactc tcacaggag tatatcaaa 
      atgtgggag aggttggag aagttggag gggttgttg 
      tacgagcaa cagagtgtc atttcccaa ttgttggat 
      ttttacacc acggggggc tgtcgcaac atgggggcg 
      ggagcaaca gctggggaa ctggaagtg attcgtgag 
      attctgggc aatgggact gtggaggac gggctggag 
      gaggggcaa gacgtgtca aggacacaa gagttggag 
      ttggaggag ttgcatgtg ccggatgag gcgttctat 
      cgcagcttg aaccaggcg tatcgggag gacttgggg 
      gggcaaggg tcacagttg gggatgatt tgggaggag 
      aaggaggaa actgacgac aacaatgag aagaaaaat 
      gattgttgt ttggttgaa ggcgaggag gtttttagt 
      gctttggac tacaaggac tatcggggg aattccccg 
      tgtgttagt gaacaggat atagctcaa ttgtcacgc 
      attgactcc atagatcac gtcgccatt gaaaaatta 
      aacgaactg gaccgtttg agcagtaaa ttgcaacaa 
      gactcagat aatattaaa caacttcaa cgtaaactc 
      ctcgaatct cctctttcc atgtcgcaa gacgacgaa 
      ccaaaagac gatcctaac tggagtttc tccaattcc 
      aaccaaacc gacctcctc atcctcaaa accgaactt 
      ccaaacttc gccccacgt cctagttcc cgcctctcc 
      gtcactgat agtgtcatt gccactgat aacgaagtc 
      gccgaagtg ctcgccggt ggtttacgc gggagcacc 
      cccacgtcc ccccggcgc gtcgcctcc tgcaccgcc 
      cccttcacc accgtcacc ggccgcctc gcctacagc 
      gccgctgtc caaaacatc gactccaaa aacccgttt 
      tacagtagt ctagtggcc gcctcctac gagaccatc 
      ccctgctcc accccttcg atcttcaac gcgcgcgcc 
      gaaaccgcc ccctcgccc tccctctcc atccgcacg 
      cgccaagac ggctacatt gcaacgaac gaccagaaa 
      ggttcaccg agtccgccc ccaccggcc cccgagccc 
      aacccagtc ttcctagcc actgagccg gcgccacag 
      cagtcaatt aagtcgccc aaaagctcc cccaagcgc 
      aaaaacgtc gtcaaatcc gactcaggg ctgtcgtca 
      atgagtggg tggtcaagt ctcgagaaa tcaccagga 
      agtccgaaa atcaacaaa gggcaccac catgacaac 
      accatccta cctggtggc caccacctt tcggcgttc 
      acgacggtg aagtcaccg tgtagtttg gagacgagc 
      gaagggtac ggtactttt gtcccagca ccggcccca 
      gcctccgat attaatgtg agtccaagg aggaaaaaa 
      cacaccaag gcgaattct ttcacttgg aacgaccaa 
      ccagcgatt tactccgtg gcggggagc aaccgcgag 
      acttacact tctgtctat actagtaac agtgccgat 
      tatatcacc cagactatc aattacccg gatttggtc 
      gagccttac gaaaacaac gaattgcaa cgaactact 
      tcaggtacc accgagagg aggaggtca ctctcgagg 
      atgttctcc acgagtaac ggcgacggg gtcgctaac 
      catgaaggt tacaagacg gcaatgcaa cgcacgatg 
      ttccccacc ggtaacatc accgacgcc ttgtcgtac 
      tacccgacc tccaccaga tacgacgag tacaacgag 
      gcctggctt aacccccaa gaacaacaa gaaagtccg 
      agcagttcc atgcgttcc acggaaccc tcggagtac 
      aaccaaagc ccctacctg gaccagagg agaatctac 
      gagaaccag gacaggtgg aaccaacgg caggagatc 
      ccttacttg gagctcgac ctccggaac cggaacgtc 
      cctgaatat gtggtccaa caaaacgag aacaagttc 
      tttgacccc agctcggtc ataatcact cagtcgggc 
      tatattacc atcaattcg gacacaatc gactcatcc 
      cgggaggaa aaaaagaaa ttcaagcgg ggtaatacg 
      ttaaaatcg gccatgagt tcggtaagt cattggtta 
      ccagacctg cacctcacc aagcgacac cgctcgtat 
      tccttaccg tcgggggtc aggcgggag gatttgtcc 
      aaacctaaa agtaacgtc ggcggtcgc aagaagaag 
      aaaaacgcg attgtgtcc acggtttcg gggattttg 
      caaaaagcc aagcggaaa agctacttc caccaccac 
      acccagtca ctctctgac cccgaacag tccgagaac 
      gagtgggtg ttacaacgg gccagttct gtggtctct 
      gaagatgat agaagtgag gatagttac agtatgttt 
      gaaggacaa gctgagcga gacctcttc gccaaagtg 
      gcgaaaccc aaacccgag gacctccaa gtggttaaa 
      gtgtctcca ccaccactc aacgaccac gtggaggag 
      gaagtctcc ggcgggagc gcctccagt agcattttc 
      cccacggtc ggcgaaccg tcgaagaag agttccgag 
      tcggagaaa agtgacgaa acggtcatc accgaaagc 
      aagatggtc gtggtggtt ggaggcgcc tccatggag 
      ttcgccgtg tcccgagcc ttgggcaaa taccgccaa 
      cagaagcaa acctctctc cccgacgat ctctccttg 
      gaggaaccc caaggggtc gtgatcgag gaaatcgtc 
      gaagaggaa ccaaaagtg gacaagttg gtcgaacaa 
      atcagtact gaaagcgaa gtcagtcac acggaaccg 
      gtcctaagg gcccaaaat cgctacttg cccaaacac 
      caacaaagt ttggaaata ccaggacat aaggaggac 
      gatgacagt cggagcacg cactcctgg cggagcacc 
      tcccgagtg tcctcgcgc cgccaaagc accgaagac 
      agcatcgac agtgaggac gagtggtat tgctacgag 
      ttgcggaag ctggaggag atggaacgt cagtcgcag 
      cttgaagcc gagatgggt gaaaccctc cgggaggag 
      cccgagttg gaggaggag tacgaaccg ttcgaggag 
      gtcaaggag cggatgtcc ttcgtgttg cgagagcta 
      cgcatgaag gccaaggtc gtggagggg gttctggag 
      gagggcgac aacaacctc cacaccctc caagaggcg 
      aagaaccgc gaacgcaaa tccatccat ttggaggag 
      gcgcacatg ttgttcgac aaaatcgcc caaaacatc 
      aaggaggag gaggaggag caagccaag gacgaggaa 
      cactcctcg ggggagacc tccgggccg gactccccc 
      cagcagagc gagaacgag gaggagaac acggaggag 
      tttgccagg tgtcacgtc accccccgt gaggaggag 
      caagctgag gaggcttcc gccgtcccc gaaatccag 
      gtcgaaccg gaagtggcg aagcccgag accaaggaa 
      accgccggg agtaagtgg aagctcttg aaggctttg 
      aaggagcgg aaggcggag gagaagagc aaccaggac 
      aaggttgag ccagctagt gagaaggac aaagtgagt 
      atgctgggg atggtgcaa aagttctac gaaatccac 
      gtcaaaacc tag
    mRNA sequence
    • >TC031720 RA
      catgtgaga tcgaaacga accgtaaag ctgaccggt 
      ctcccctgt taagaagcg tcttaacac ggtggacct 
      tttactttt tttattttg taaattttg gccactaat 
      tgctgattt tataggact tggggggcg tttaggagc 
      gtaatagat gttgaagca tgccttctt ccacacgga 
      cgaaacaat gactttagt gttgccagt gtctcgtga 
      tgttcgcga gcacgccaa tccgcacca tgccgcgaa 
      aaaagacta aaatgtagt gtaaattct ggtgaaaaa 
      tgagcgaaa acgtgcacc ggatggccg acggtacca 
      tggcacccg tcaactacg acgccaacg ttgactatg 
      tcatcaccg aatacatgg aacggctcg gaaccagac 
      tcaacatcc ttgaaacgg agctgaaat acgcctggc 
      gtgcccttg atttactct cacaggagt atatcaaaa 
      tgtgggaga ggttggaga agttggagg ggttgttgt 
      acgagcaac agagtgtca tttcccaat tgttggatt 
      tttacacca cggggggct gtcgcaaca tgggggcgg 
      gagcaacag ctggggaac tggaagtga ttcgtgaga 
      ttctgggca atgggactg tggaggacg ggctggagg 
      aggggcaag acgtgtcaa ggacacaag agttggagt 
      tggaggagt tgcatgtgc cggatgagg cgttctatc 
      gcagcttga accaggcgt atcgggagg acttggggg 
      ggcaagggt cacagttgg ggatgattt gggaggaga 
      aggaggaaa ctgacgaca acaatgaga agaaaaatg 
      attgttgtt tggttgaag gcgaggagg tttttagtg 
      ctttggact acaaggact atcggggga attccccgt 
      gtgttagtg aacaggata tagctcaat tgtcacgca 
      ttgactcca tagatcacg tcgccattg aaaaattaa 
      acgaactgg accgtttga gcagtaaat tgcaacaag 
      actcagata atattaaac aacttcaac gtaaactcc 
      tcgaatctc ctctttcca tgtcgcaag acgacgaac 
      caaaagacg atcctaact ggagtttct ccaattcca 
      accaaaccg acctcctca tcctcaaaa ccgaacttc 
      caaacttcg ccccacgtc ctagttccc gcctctccg 
      tcactgata gtgtcattg ccactgata acgaagtcg 
      ccgaagtgc tcgccggtg gtttacgcg ggagcaccc 
      ccacgtccc cccggcgcg tcgcctcct gcaccgccc 
      ccttcacca ccgtcaccg gccgcctcg cctacagcg 
      ccgctgtcc aaaacatcg actccaaaa acccgtttt 
      acagtagtc tagtggccg cctcctacg agaccatcc 
      cctgctcca ccccttcga tcttcaacg cgcgcgccg 
      aaaccgccc cctcgccct ccctctcca tccgcacgc 
      gccaagacg gctacattg caacgaacg accagaaag 
      gttcaccga gtccgcccc caccggccc ccgagccca 
      acccagtct tcctagcca ctgagccgg cgccacagc 
      agtcaatta agtcgccca aaagctccc ccaagcgca 
      aaaacgtcg tcaaatccg actcagggc tgtcgtcaa 
      tgagtgggt ggtcaagtc tcgagaaat caccaggaa 
      gtccgaaaa tcaacaaag ggcaccacc atgacaaca 
      ccatcctac ctggtggcc accaccttt cggcgttca 
      cgacggtga agtcaccgt gtagtttgg agacgagcg 
      aagggtacg gtacttttg tcccagcac cggccccag 
      cctccgata ttaatgtga gtccaagga ggaaaaaac 
      acaccaagg cgaattctt tcacttgga acgaccaac 
      cagcgattt actccgtgg cggggagca accgcgaga 
      cttacactt ctgtctata ctagtaaca gtgccgatt 
      atatcaccc agactatca attacccgg atttggtcg 
      agccttacg aaaacaacg aattgcaac gaactactt 
      caggtacca ccgagagga ggaggtcac tctcgagga 
      tgttctcca cgagtaacg gcgacgggg tcgctaacc 
      atgaaggtt acaagacgg caatgcaac gcacgatgt 
      tccccaccg gtaacatca ccgacgcct tgtcgtact 
      acccgacct ccaccagat acgacgagt acaacgagg 
      cctggctta acccccaag aacaacaag aaagtccga 
      gcagttcca tgcgttcca cggaaccct cggagtaca 
      accaaagcc cctacctgg accagagga gaatctacg 
      agaaccagg acaggtgga accaacggc aggagatcc 
      cttacttgg agctcgacc tccggaacc ggaacgtcc 
      ctgaatatg tggtccaac aaaacgaga acaagttct 
      ttgacccca gctcggtca taatcactc agtcgggct 
      atattacca tcaattcgg acacaatcg actcatccc 
      gggaggaaa aaaagaaat tcaagcggg gtaatacgt 
      taaaatcgg ccatgagtt cggtaagtc attggttac 
      cagacctgc acctcacca agcgacacc gctcgtatt 
      ccttaccgt cgggggtca ggcgggagg atttgtcca 
      aacctaaaa gtaacgtcg gcggtcgca agaagaaga 
      aaaacgcga ttgtgtcca cggtttcgg ggattttgc 
      aaaaagcca agcggaaaa gctacttcc accaccaca 
      cccagtcac tctctgacc ccgaacagt ccgagaacg 
      agtgggtgt tacaacggg ccagttctg tggtctctg 
      aagatgata gaagtgagg atagttaca gtatgtttg 
      aaggacaag ctgagcgag acctcttcg ccaaagtgg 
      cgaaaccca aacccgagg acctccaag tggttaaag 
      tgtctccac caccactca acgaccacg tggaggagg 
      aagtctccg gcgggagcg cctccagta gcattttcc 
      ccacggtcg gcgaaccgt cgaagaaga gttccgagt 
      cggagaaaa gtgacgaaa cggtcatca ccgaaagca 
      agatggtcg tggtggttg gaggcgcct ccatggagt 
      tcgccgtgt cccgagcct tgggcaaat accgccaac 
      agaagcaaa cctctctcc ccgacgatc tctccttgg 
      aggaacccc aaggggtcg tgatcgagg aaatcgtcg 
      aagaggaac caaaagtgg acaagttgg tcgaacaaa 
      tcagtactg aaagcgaag tcagtcaca cggaaccgg 
      tcctaaggg cccaaaatc gctacttgc ccaaacacc 
      aacaaagtt tggaaatac caggacata aggaggacg 
      atgacagtc ggagcacgc actcctggc ggagcacct 
      cccgagtgt cctcgcgcc gccaaagca ccgaagaca 
      gcatcgaca gtgaggacg agtggtatt gctacgagt 
      tgcggaagc tggaggaga tggaacgtc agtcgcagc 
      ttgaagccg agatgggtg aaaccctcc gggaggagc 
      ccgagttgg aggaggagt acgaaccgt tcgaggagg 
      tcaaggagc ggatgtcct tcgtgttgc gagagctac 
      gcatgaagg ccaaggtcg tggaggggg ttctggagg 
      agggcgaca acaacctcc acaccctcc aagaggcga 
      agaaccgcg aacgcaaat ccatccatt tggaggagg 
      cgcacatgt tgttcgaca aaatcgccc aaaacatca 
      aggaggagg aggaggagc aagccaagg acgaggaac 
      actcctcgg gggagacct ccgggccgg actcccccc 
      agcagagcg agaacgagg aggagaaca cggaggagt 
      ttgccaggt gtcacgtca ccccccgtg aggaggagc 
      aagctgagg aggcttccg ccgtccccg aaatccagg 
      tcgaaccgg aagtggcga agcccgaga ccaaggaaa 
      ccgccggga gtaagtgga agctcttga aggctttga 
      aggagcgga aggcggagg agaagagca accaggaca 
      aggttgagc cagctagtg agaaggaca aagtgagta 
      tgctgggga tggtgcaaa agttctacg aaatccacg 
      tcaaaacct agcttagtc aagcgcttc gccttattg 
      taaccggct tttagaggt caccacgag tcaattcga 
      atccaggag tttgtgttt agtcatatc gcactcata 
      tcaatgtaa ttatcatca cggagattg acattatgc 
      agtgtttat actatcgtt gacaataac gcgagcgta 
      cgattctaa accactcta ctgtacata acgaccaag 
      acgtgatag tataacgcg ctgattttc ctactttag 
      cacctctag actggccat aatgacaat ctcgactag 
      atcggtgtt agcagtatt atgcttgac ttagggaca 
      ttgtgctaa gatttgttg atttgaata aaatctgtt 
      acccctt
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