Status
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Gene information
General information
TC032146
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000532
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10125021..10131818
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC032146
      Gene ontology

      GO terms for Tribolium

      • {{ goTerm.term }}
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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
      {{ blurIdResult }}
      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_11050

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Adult morphology
      prothoracic odoriferous glands potentially not present - Number of animals showing the phenotype: 10 (13 dpi)
      Days post injection



      abdominal odoriferous glands not present - Number of animals showing the phenotype: 10 (13 dpi)
      Days post injection



      abdominal odoriferous glands content not present - Number of animals showing the phenotype: 10 (13 dpi)
      Days post injection



      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      larval musculature random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      head capsule anterior partiallyshape broader - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      labium potentially posteriorstructure swollen - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC032146 RA
        MQSQMHFGH SFDSRWAQG GQGLSKTNL RHDSPDRDV 
        DDDRRDYKR QSSEDSHRS SHRSQSDLS HKSSDRFGD 
        DSHDYRHQD YDYNRKNRD EDKWQKENE KKKHDDDWH 
        GDRRDKYRD DKHERPQRP DSRDSRASR ESRQSRDSA 
        RDSEPRDHI GSWADTVFE PTYEEKRRD HGREDRRQV 
        PGPITKERI EADDMKNEK RGLTQLKRG QMPDKKIAE 
        KKDEKIQDG SIGENKLQD QLQDSGTKA WADAVSPNT 
        IDPENPKYM DTFEKNKSS SSTEALKQE NDLKKSDNS 
        EFKDKDDKR GRNRNYPSS KGWSGNEYH RSTNWPKRQ 
        TSRGARPGG PQRPGDFHG TDSEGSMDE VNVDGVPLK 
        SPKTVKKSE KDDKNKEIK QNEKTATLE RNKDKQDSK 
        RENYVPRGE PSRHGRGGG NFRGRGGLS KRIDGYGPP 
        PSKSPFGHH DDKEKKLSS DENSGDITI EDKAKQNQQ 
        ALSAGIIGS ARKEMGAKL DDKAKSRKP DHRRGKSKT 
        KADGKRDDE TVDSNSDNS DDKDGKGLK KTHKTNPPL 
        RGPLSMNQT RRTNAPPRL TSGEKKTHP SRNENPIPR 
        QNSSGALRS QSVIKKDEK SGEPNSLCS GIADISLKN 
        KPETEPEDG DEKVSLQSD SDGFQEVKS KKTGKERQK 
        SIDEKPGKA PIKPDKDAG KQDRKPKSS STVQLTQQQ 
        ISNIPPLMA TPVNPPPVL PQANKNPFE RPRQSKLAP 
        RFAKQRENN RLQKAQMQQ QQNICDVND MNKVNQNMN 
        VYGMKDSSS VVMPLANAW DKSLGGPPL RNIDSENVL 
        GVSGDGCKT LDHVQASQS NSPSNDKII GKSSQLQDK 
        AILDGTTPP VNTIIFENT NFKSAPGSR NSRSDKPRS 
        KLDEGMDGS VVSGFNKPI SDLLCKNDK QSESIQIPL 
        SFKEDNADI KLDFFDSEL SHLTEEKSS KNLCMPRSM 
        HAITTGNNT ITTPDALNF KIQSVKKMW ETPADHNVG 
        QEDNNSSFS SSFVSDPNT LDPNAFGKG NDTPDDNHE 
        GYSPSPNQV ASNSTTNVC KVKPTQQVS GGGAQAVLN 
        SATHQQHSG IMGPSLMGN PLSPPPLQP VIGTGVNLG 
        QPPQQYTAN QHISYQAGL GGSNQYGIS AIPSPPTVM 
        YNSTQQIQQ TGLYGPFQI DQTGVLGGQ GRSQYSQYP 
        NHYSLGQTA SSPYSTQSV YLQTQPHPP PAAQAPPDL 
        YQNISNYRL SATGPFGQN QQLNNPTTV LISSTSSNS 
        LMSASVKPS SQQISAIGT KAGGVGQAY QQQSQQSQQ 
        VYMAYEPAL QANYLQSAA GVMQRGPTG PVQNNVVPA 
        LQPSSSYYS GSTGQTGYF QQPGSSTIQ SAQLQQHQA 
        GYGLQGNVF GTHNQSHTN AGLQSFNSH FLSTPMQLA 
        AALNAQQFR SSLPTQFMK GVGSQQIGD QTGRSQQLK 
        SPGSQEVLS SVFNSGSQI SSPKLRQNC KQPPPQPSP 
        TAQHKYNLY QGVGNQQSG NMQRYPPPI QRPVNFQQM 
        GSVQQNNSA NNQKHRSNN PNNKASSRQ YYQNNSISS 
        QSEKIDDSK LNDNSLSNS NSNSVNSSV ANKTVIGTS 
        AATTVASPE ISKEAINIE ETTALKD
      CDS sequence
      • >TC032146 RA
        atgcagagt caaatgcat ttcgggcat agttttgat 
        agtcgatgg gctcaagga ggacaagga ttgtcaaaa 
        acgaatttg agacatgat agtcccgat cgtgatgtg 
        gatgatgat agaagggat tataaaaga caaagttct 
        gaagatagt caccgaagt tcacatcgg tcacaatcg 
        gacttgtct cataaatcg tcggatcgg tttggtgat 
        gactcgcac gattatagg catcaggat tacgattat 
        aacaggaag aatcgagac gaggataag tggcaaaag 
        gaaaacgaa aagaaaaag catgatgat gattggcat 
        ggtgatagg agagataag tatcgtgat gataagcat 
        gaaaggcca caaaggccc gatagtcga gattcgaga 
        gcgtctagg gaatcacgt caatcgagg gattcggca 
        cgtgattca gaaccaagg gatcatatc ggctcgtgg 
        gctgataca gtgtttgaa cctacatat gaagaaaaa 
        agacgggat catggcaga gaggaccga cgtcaagtt 
        ccaggtcca attacgaaa gaaagaata gaggctgac 
        gatatgaag aacgagaag cgaggtttg acacaattg 
        aagagaggc caaatgccc gataagaaa attgcggaa 
        aagaaagat gagaagatc caagatgga tcaattggt 
        gaaaataaa ttgcaggac cagttgcag gattctggg 
        acaaaagca tgggctgat gccgtatca cccaatact 
        atagacccc gagaatcct aaatatatg gatacgttt 
        gaaaagaat aaaagcagc tcgagcacg gaagcgctt 
        aagcaagag aatgatttg aaaaagtct gataactcg 
        gaattcaag gataaagat gataagcgc ggtagaaac 
        cgcaattat ccgtcgagt aaaggttgg agtggtaac 
        gaatatcac cggtctact aattggccg aaaagacaa 
        acaagtcga ggagcgcgc cccggaggg cctcagaga 
        ccgggtgat ttccacgga acggattca gaaggttca 
        atggacgag gtaaatgtt gatggtgtc ccactaaag 
        agtcctaaa accgttaaa aaatcggag aaggacgac 
        aaaaataag gaaatcaaa caaaacgaa aagaccgcg 
        acgttggaa cgaaacaaa gataagcag gacagcaaa 
        cgcgagaat tacgtaccg agaggggaa ccgtcgagg 
        catggacgt ggaggtggc aatttccgt ggtagaggt 
        ggtttaagt aaaagaatc gatggttac ggtcctcca 
        ccgtcgaaa agtcctttc ggtcatcac gacgataaa 
        gaaaaaaaa ttatcaagt gatgagaat tccggtgat 
        ataacaatt gaagacaaa gctaagcaa aatcagcaa 
        gcactatct gctggaatc atcggaagt gctcgaaag 
        gaaatggga gccaagttg gatgacaaa gccaagagt 
        cggaaacct gaccacaga agaggaaaa tcgaagact 
        aaagctgat gggaaacgg gacgacgaa accgttgat 
        tctaattcg gataattcc gatgataaa gatggcaaa 
        ggtttgaaa aaaacgcac aaaactaac cctcctctg 
        agaggccct ctaagcatg aatcaaacc cggcgcact 
        aacgctccg ccacgttta accagtggt gagaaaaaa 
        actcatcca tctcgtaat gaaaatccc attccgaga 
        caaaattca agcggagcg ttgcgttct caaagtgta 
        ataaagaag gatgaaaag tcgggtgaa ccgaacagt 
        ttgtgtagc ggcattgcc gatatttct ctcaagaat 
        aaaccggag accgaacca gaagatggt gatgaaaaa 
        gtctccctt cagagtgat tctgatggt ttccaagaa 
        gtcaaaagt aagaaaact gggaaggaa cgtcagaaa 
        tcaatcgac gagaaaccg ggcaaggcg ccgataaag 
        ccggacaag gatgcagga aaacaggac cggaaacct 
        aagagtagt tcgactgta caattaaca cagcaacaa 
        atctcgaac attccgcca ctgatggca actcctgtt 
        aatccgcca ccggtttta ccacaagct aataaaaat 
        ccgtttgaa aggccgcgt cagagtaaa ttggcgccg 
        aggtttgcg aagcaaaga gaaaataat agactgcaa 
        aaagcgcaa atgcaacag caacagaat atttgtgac 
        gtgaatgat atgaacaaa gtgaatcaa aacatgaac 
        gtttacggt atgaaagat tcgtcaagt gttgtgatg 
        ccgttggcg aatgcttgg gataagtcg ttagggggg 
        cccccgttg agaaatatc gattcggaa aatgtgttg 
        ggagtttct ggggatgga tgcaagact ttggaccat 
        gtccaagcg agtcagagc aacagcccc agcaatgat 
        aagattatc ggtaaaagc agtcagttg caggacaag 
        gcaattctc gatggtacg acgcctcct gtcaacacc 
        atcattttc gaaaacaca aattttaaa tcagctcct 
        ggttctcga aattcaaga tccgataag ccacgttca 
        aagttggat gaaggcatg gacggatct gtggtatca 
        ggtttcaat aaacctata tcggatttg ctttgtaaa 
        aacgacaaa cagtccgag tcgattcag attccgctc 
        tcttttaaa gaagataat gccgatata aagttggat 
        ttttttgat tccgaactg tctcatctg accgaagag 
        aaaagttcg aaaaatttg tgtatgcca cgttcgatg 
        cacgccatc acaacgggc aataacacg attacaacg 
        cctgatgcg ttgaatttt aaaatacaa tcagtgaaa 
        aaaatgtgg gaaacccca gctgatcac aatgttgga 
        caagaagat aacaattcg agtttctcg agttcgttt 
        gtttcggat cctaatacg ttggatccg aacgctttc 
        ggtaaaggg aacgatact cccgacgat aatcacgaa 
        ggttatagt ccttctccc aatcaagtt gcttcaaac 
        agcacaaca aatgtgtgc aaggtaaaa cctactcag 
        caagtgtca ggtggtggt gcccaagct gtgttaaat 
        tcagcgact catcaacag cattcgggc atcatggga 
        ccaagtctg atggggaat cccttgtca ccgcctcct 
        ctacagccg gtcatcgga actggcgtt aatttaggc 
        caaccccct cagcaatac acggccaat caacacatt 
        agctatcaa gcgggtctt ggtggcagt aatcaatac 
        ggcatctcg gccatacct tcaccgcca accgttatg 
        tataattcg actcaacaa atacaacag acgggtctc 
        tacggacca ttccagatc gatcagact ggagtgtta 
        ggtggtcaa ggacgttcc caatattcc caatatcct 
        aaccattat agtttgggt cagacagcc tctagtccg 
        tattcgacg caatcggtg tatttacag acccaacct 
        cacccccct cctgcggcc caagcgccg cccgacctt 
        taccaaaat attagtaat tacagactg tctgcgaca 
        ggaccgttc ggacaaaat caacaattg aataatccc 
        acaactgtc ctcatttcg tccacttcc tccaattcg 
        ttgatgtcc gcttcggtc aaacccagc agccaacaa 
        atcagcgcg attggtacc aaagctggc ggagttggg 
        caagcctac caacagcag tctcaacag agccagcaa 
        gtgtacatg gcgtacgag cccgcgctt caggccaat 
        tatttgcaa agtgctgca ggtgtgatg caaagagga 
        cccactggt cccgtgcag aacaatgtg gttcccgct 
        ttgcagcct tcttcatca tattattca ggatcgaca 
        ggtcagaca gggtatttc caacagcct ggaagttct 
        actattcag tctgctcag ttacagcaa caccaagct 
        gggtacgga cttcaaggc aacgttttt ggtacccat 
        aaccagtct catactaac gccggattg caaagtttc 
        aactcgcat tttttatcg actccaatg cagttggct 
        gcagcacta aatgcgcaa caatttcgg tccagcctt 
        ccaacacag tttatgaaa ggcgtcggc agccaacaa 
        attggtgat caaacagga aggtcacaa caactcaaa 
        agccccggc agtcaagaa gtgctgtcg tcggtcttc 
        aactctggt tcgcagatt tcgtcccct aagttaagg 
        cagaactgt aaacaaccc ccgcctcaa ccgagccca 
        acagcccaa cataaatac aatctctat caaggggtg 
        ggcaaccaa cagtctgga aatatgcag aggtaccct 
        cctccaatt cagcgaccc gtcaatttc cagcaaatg 
        ggttccgta caacaaaac aattctgcc aacaaccaa 
        aagcatcgt tctaacaat cctaacaat aaagcttct 
        tctcgacaa tattatcaa aataattca atttcatct 
        cagagtgaa aaaatcgac gacagtaag ctgaatgat 
        aattccctg agcaacagc aattccaac agcgttaat 
        tcgtctgtt gctaacaaa accgtaatt gggacaagt 
        gccgcaact acggtagct tctccggag atttcaaaa 
        gaggctata aatatagaa gaaaccacc gctttgaag 
        gattaa
      mRNA sequence
      • >TC032146 RA
        ccaaacgat acaggaagt gacgagaaa agttcaaat 
        tccttcaga caattgacc caaatcgag ggtaaaaac 
        ttcgcacct cgcaagcac gtcaaaggg tctgaaaga 
        gatcgtgac gttcgcgaa cagaacggt ccgagtttt 
        tcgcgacat tttcaattg cctccgagg tttcaaaaa 
        caaaggaat aacccgaat tcgaatatg cagagtcaa 
        atgcatttc gggcatagt tttgatagt cgatgggct 
        caaggagga caaggattg tcaaaaacg aatttgaga 
        catgatagt cccgatcgt gatgtggat gatgataga 
        agggattat aaaagacaa agttctgaa gatagtcac 
        cgaagttca catcggtca caatcggac ttgtctcat 
        aaatcgtcg gatcggttt ggtgatgac tcgcacgat 
        tataggcat caggattac gattataac aggaagaat 
        cgagacgag gataagtgg caaaaggaa aacgaaaag 
        aaaaagcat gatgatgat tggcatggt gataggaga 
        gataagtat cgtgatgat aagcatgaa aggccacaa 
        aggcccgat agtcgagat tcgagagcg tctagggaa 
        tcacgtcaa tcgagggat tcggcacgt gattcagaa 
        ccaagggat catatcggc tcgtgggct gatacagtg 
        tttgaacct acatatgaa gaaaaaaga cgggatcat 
        ggcagagag gaccgacgt caagttcca ggtccaatt 
        acgaaagaa agaatagag gctgacgat atgaagaac 
        gagaagcga ggtttgaca caattgaag agaggccaa 
        atgcccgat aagaaaatt gcggaaaag aaagatgag 
        aagatccaa gatggatca attggtgaa aataaattg 
        caggaccag ttgcaggat tctgggaca aaagcatgg 
        gctgatgcc gtatcaccc aatactata gaccccgag 
        aatcctaaa tatatggat acgtttgaa aagaataaa 
        agcagctcg agcacggaa gcgcttaag caagagaat 
        gatttgaaa aagtctgat aactcggaa ttcaaggat 
        aaagatgat aagcgcggt agaaaccgc aattatccg 
        tcgagtaaa ggttggagt ggtaacgaa tatcaccgg 
        tctactaat tggccgaaa agacaaaca agtcgagga 
        gcgcgcccc ggagggcct cagagaccg ggtgatttc 
        cacggaacg gattcagaa ggttcaatg gacgaggta 
        aatgttgat ggtgtccca ctaaagagt cctaaaacc 
        gttaaaaaa tcggagaag gacgacaaa aataaggaa 
        atcaaacaa aacgaaaag accgcgacg ttggaacga 
        aacaaagat aagcaggac agcaaacgc gagaattac 
        gtaccgaga ggggaaccg tcgaggcat ggacgtgga 
        ggtggcaat ttccgtggt agaggtggt ttaagtaaa 
        agaatcgat ggttacggt cctccaccg tcgaaaagt 
        cctttcggt catcacgac gataaagaa aaaaaatta 
        tcaagtgat gagaattcc ggtgatata acaattgaa 
        gacaaagct aagcaaaat cagcaagca ctatctgct 
        ggaatcatc ggaagtgct cgaaaggaa atgggagcc 
        aagttggat gacaaagcc aagagtcgg aaacctgac 
        cacagaaga ggaaaatcg aagactaaa gctgatggg 
        aaacgggac gacgaaacc gttgattct aattcggat 
        aattccgat gataaagat ggcaaaggt ttgaaaaaa 
        acgcacaaa actaaccct cctctgaga ggccctcta 
        agcatgaat caaacccgg cgcactaac gctccgcca 
        cgtttaacc agtggtgag aaaaaaact catccatct 
        cgtaatgaa aatcccatt ccgagacaa aattcaagc 
        ggagcgttg cgttctcaa agtgtaata aagaaggat 
        gaaaagtcg ggtgaaccg aacagtttg tgtagcggc 
        attgccgat atttctctc aagaataaa ccggagacc 
        gaaccagaa gatggtgat gaaaaagtc tcccttcag 
        agtgattct gatggtttc caagaagtc aaaagtaag 
        aaaactggg aaggaacgt cagaaatca atcgacgag 
        aaaccgggc aaggcgccg ataaagccg gacaaggat 
        gcaggaaaa caggaccgg aaacctaag agtagttcg 
        actgtacaa ttaacacag caacaaatc tcgaacatt 
        ccgccactg atggcaact cctgttaat ccgccaccg 
        gttttacca caagctaat aaaaatccg tttgaaagg 
        ccgcgtcag agtaaattg gcgccgagg tttgcgaag 
        caaagagaa aataataga ctgcaaaaa gcgcaaatg 
        caacagcaa cagaatatt tgtgacgtg aatgatatg 
        aacaaagtg aatcaaaac atgaacgtt tacggtatg 
        aaagattcg tcaagtgtt gtgatgccg ttggcgaat 
        gcttgggat aagtcgtta ggggggccc ccgttgaga 
        aatatcgat tcggaaaat gtgttggga gtttctggg 
        gatggatgc aagactttg gaccatgtc caagcgagt 
        cagagcaac agccccagc aatgataag attatcggt 
        aaaagcagt cagttgcag gacaaggca attctcgat 
        ggtacgacg cctcctgtc aacaccatc attttcgaa 
        aacacaaat tttaaatca gctcctggt tctcgaaat 
        tcaagatcc gataagcca cgttcaaag ttggatgaa 
        ggcatggac ggatctgtg gtatcaggt ttcaataaa 
        cctatatcg gatttgctt tgtaaaaac gacaaacag 
        tccgagtcg attcagatt ccgctctct tttaaagaa 
        gataatgcc gatataaag ttggatttt tttgattcc 
        gaactgtct catctgacc gaagagaaa agttcgaaa 
        aatttgtgt atgccacgt tcgatgcac gccatcaca 
        acgggcaat aacacgatt acaacgcct gatgcgttg 
        aattttaaa atacaatca gtgaaaaaa atgtgggaa 
        accccagct gatcacaat gttggacaa gaagataac 
        aattcgagt ttctcgagt tcgtttgtt tcggatcct 
        aatacgttg gatccgaac gctttcggt aaagggaac 
        gatactccc gacgataat cacgaaggt tatagtcct 
        tctcccaat caagttgct tcaaacagc acaacaaat 
        gtgtgcaag gtaaaacct actcagcaa gtgtcaggt 
        ggtggtgcc caagctgtg ttaaattca gcgactcat 
        caacagcat tcgggcatc atgggacca agtctgatg 
        gggaatccc ttgtcaccg cctcctcta cagccggtc 
        atcggaact ggcgttaat ttaggccaa ccccctcag 
        caatacacg gccaatcaa cacattagc tatcaagcg 
        ggtcttggt ggcagtaat caatacggc atctcggcc 
        ataccttca ccgccaacc gttatgtat aattcgact 
        caacaaata caacagacg ggtctctac ggaccattc 
        cagatcgat cagactgga gtgttaggt ggtcaagga 
        cgttcccaa tattcccaa tatcctaac cattatagt 
        ttgggtcag acagcctct agtccgtat tcgacgcaa 
        tcggtgtat ttacagacc caacctcac ccccctcct 
        gcggcccaa gcgccgccc gacctttac caaaatatt 
        agtaattac agactgtct gcgacagga ccgttcgga 
        caaaatcaa caattgaat aatcccaca actgtcctc 
        atttcgtcc acttcctcc aattcgttg atgtccgct 
        tcggtcaaa cccagcagc caacaaatc agcgcgatt 
        ggtaccaaa gctggcgga gttgggcaa gcctaccaa 
        cagcagtct caacagagc cagcaagtg tacatggcg 
        tacgagccc gcgcttcag gccaattat ttgcaaagt 
        gctgcaggt gtgatgcaa agaggaccc actggtccc 
        gtgcagaac aatgtggtt cccgctttg cagccttct 
        tcatcatat tattcagga tcgacaggt cagacaggg 
        tatttccaa cagcctgga agttctact attcagtct 
        gctcagtta cagcaacac caagctggg tacggactt 
        caaggcaac gtttttggt acccataac cagtctcat 
        actaacgcc ggattgcaa agtttcaac tcgcatttt 
        ttatcgact ccaatgcag ttggctgca gcactaaat 
        gcgcaacaa tttcggtcc agccttcca acacagttt 
        atgaaaggc gtcggcagc caacaaatt ggtgatcaa 
        acaggaagg tcacaacaa ctcaaaagc cccggcagt 
        caagaagtg ctgtcgtcg gtcttcaac tctggttcg 
        cagatttcg tcccctaag ttaaggcag aactgtaaa 
        caacccccg cctcaaccg agcccaaca gcccaacat 
        aaatacaat ctctatcaa ggggtgggc aaccaacag 
        tctggaaat atgcagagg taccctcct ccaattcag 
        cgacccgtc aatttccag caaatgggt tccgtacaa 
        caaaacaat tctgccaac aaccaaaag catcgttct 
        aacaatcct aacaataaa gcttcttct cgacaatat 
        tatcaaaat aattcaatt tcatctcag agtgaaaaa 
        atcgacgac agtaagctg aatgataat tccctgagc 
        aacagcaat tccaacagc gttaattcg tctgttgct 
        aacaaaacc gtaattggg acaagtgcc gcaactacg 
        gtagcttct ccggagatt tcaaaagag gctataaat 
        atagaagaa accaccgct ttgaaggat taagtgttg 
        tcttttatt gttttaagc actaaacag tttgtatcg 
        tgttaacca atttccaaa ttttgtaca ttattacta 
        attaatgaa aggaaccgc aaaaatgta tttattttt 
        tccattata ttaagtatt gaagttttc ttgtattac 
        taatagtaa ttatttatt tttcgtggt actgggact 
        tgatttgtg accggtttt tccataaat tctttgtac 
        accgatttt agaactagg agttgaagt tttatgctt 
        tgcctgaaa tataaaaat agatttttt tggtaaacg 
        tatatcctg tatcttatt gtactggtc cattgttaa 
        ctgggccca gtacgatag tttagccag accttagta 
        gttttttta ctgtgctct gtaaaaatg ttattactg 
        tgtgcactt gccaaacga tagcttcaa ctgcccaca 
        gtaacagaa atttttgag ccctttgcc gtgcaatgc 
        cgacagtgg ttctagcaa ttgtagttt tgtgatatg 
        ttaagtgta tagatattt gaaatcaag gcattgaac 
        tcgcaaatc tcaaatttc ggtaaatgt tttctacaa 
        tttgtctcc aaatcgtgg agcttctaa tttttctcg 
        agattgtat aggcttgcc taaccaact tagcgacac 
        aatctcacc tgtagctca ttagcagtg ttagtaaat 
        cgtagccag catggaatg tatcgtgta aaaattatt 
        cagtgagtg aaaaactgc actaatgtt ttatcacac 
        aaactccaa gctttatta aacctcagt gttgacgga 
        aattcgact gaaaagact gcattcgga aggtgaagt 
        gtgtgacct aaaagactc tggattcgc tctgttttg 
        ttctcttct accggcatc agcaaatta ctcagatta 
        tgaaacaaa attgtatat ctttggtta agatattta 
        attctataa atattatta cttcgtaaa ataaatggt 
        ttaaataca g
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