Status
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Gene information
General information
TC015701 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5497282..5501172
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015701
    iBeetle screen iB_11048

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    vitellarium degenerated - Number of animals showing the phenotype: 3

    vitellogenic egg chamber - Number of animals showing the phenotype: 3
    previtellogenic egg chamber not present - Number of animals showing the phenotype: 1

    previtellogenic egg chamber degenerated - Number of animals showing the phenotype: 3
    mature egg not present - Number of animals showing the phenotype: 4
    lateral oviduct degenerated - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015701 RA
      MAGEEHVEI VENGENHQK TNKRKRESS VLDNFTKLT 
      NGQEKVRVK AGIDLIRHL TSDKNGEQS NDELKYALG 
      RLIRGLGSS KIHAKTGFF AALVALLNL KTITIEEIF 
      THVEKELHK GGSNSKSEN ADICSGQIL LCGAILRSN 
      LYESCDEKE KIKVLEMLL KAGKERSYL NLASCKFLT 
      DLFQQVEQK EFETVIFPL IKNDLAKPW QDQNLDSLF 
      LFLQIQNHF PGFLHAKFL KNNLGTEEL ICSESLYDL 
      ALTLTSIPR VSSLNHPIF DLFIKTLSQ TDLTVSFFN 
      ELDRTLIRQ NRNKFFVAT KLLTGILDE LNDFSLIPE 
      LLTRNYLQQ LLSTYKTAV GKQKDKEFQ NLTQKLFET 
      LVSCLKKDG VKSKIKIKV LKTLLFFPG TFIFEKVTR 
      SKLVQNITA TLNTEGVKK LAGIYRDVI LATNDKVIN 
      ENSTEKWWN VDRIYASHL LVKLLNHPS MHENNDWKV 
      DQLVFLMKL GLLKDDNIG NDLADSFRE TFYSSLDLK 
      LSKLDDLRQ ILSKIAQEF DALATLVRQ PLSDETAEM 
      WQRTLDVAT KLQNSSKKK NSVFHTLFL HLGLQLFNG 
      NNAKLASDS LQELIICYD KTKKKSVDE SELAWIEVV 
      VDLFLNLLS HNSHLLRSV ISCVFPHLC KYMTGSAIH 
      QILGVLDPK SDSNPLSRI DESSSDEEV EEEEEDEDE 
      EEEEISDEE EEEPVNDKL RMALHQALG ASVDSEAES 
      VDLDDLDDE EGEKLDEAL SQAFKQFKP NLGRRKKQK 
      KDEEALTHF RIRVLDLIE IYLNSNPTM ILTLEIMLP 
      LLQCLEFCI RDDHQKPLQ DRLKSCLKK LSSLKKFDD 
      CEGVNEEVL VELLKSLLE KGTRNVIII QEMAEKLAE 
      CCIFVINCS EFVPKKKKI KAVIKDGLE AFFTRRDCV 
      IPYVLFKNL VQTNWEGVL DLVPQLFGF VFANSIRPF 
      RRGQAVELL RLFFSNHRF FSTHCEQVK SIEDHTRNF 
      LRDCVDFFR EFYANPTER KVKEKFLAS LFELLAQLK 
      SYEFESWAV LGDAVRDCR SILTFSKDT KVAFNKLCS 
      SLKISNVVE MKQKTVKLN TVEECESEE KEEKRRKKK 
      LSKDKLKLK KESKELRLQ SLSEGLKRK FEWEEGEEE 
      AKKAKTDDF
    CDS sequence
    • >TC015701 RA
      atggcgggc gaagagcac gtggaaatt gtggaaaac 
      ggtgaaaat catcaaaaa acaaataaa agaaaacgt 
      gaatcgtcc gttttggac aatttcacc aaattaaca 
      aacgggcaa gagaaagtg cgggttaaa gccggcatt 
      gatttaatc cggcattta acaagtgac aaaaacggg 
      gaacaaagt aacgatgag ttgaaatac gcactaggg 
      cgtttgata cgtggcctc ggctcgtct aaaattcac 
      gcaaaaact ggctttttt gccgctttg gttgcctta 
      cttaacctt aaaactatc acgattgag gagattttc 
      acacacgtg gagaaggaa ttgcacaaa gggggcagc 
      aacagtaaa agtgaaaat gccgatatt tgttcagga 
      cagattttg ctctgtggg gctattctt aggtctaat 
      ttgtacgaa agctgtgac gaaaaagaa aaaattaaa 
      gttttggaa atgttgctc aaggccggg aaagaaaga 
      tcatacttg aatctagcc tcctgtaaa tttttgacc 
      gatttattt cagcaggtt gagcaaaaa gaattcgaa 
      accgtgatt tttccactc attaaaaac gatttagcg 
      aaaccgtgg caagaccaa aacttggac tcacttttt 
      ttgtttcta caaatacaa aatcatttt cccggcttt 
      ttacatgcg aaattcttg aaaaataat ttagggacc 
      gaagaatta atttgtagc gaaagtttg tacgacttg 
      gccctgact ttgacttct attccacga gtatcgtcc 
      ttaaatcac ccaatcttt gacttgttt atcaaaacc 
      ttatcacag acagactta acagttagt tttttcaac 
      gaattagat cgcaccttg attaggcaa aaccgtaac 
      aaatttttc gtcgcaact aaactcttg accggaatt 
      ttggacgaa cttaacgat ttttcccta attcccgaa 
      cttttgaca cgaaattac ctccaacaa ttgctaagc 
      acttacaag acggctgtt ggaaaacaa aaagataaa 
      gaattccaa aatctgacc caaaaatta ttcgaaaca 
      ctcgtcagt tgtttgaaa aaagacgga gtcaagtcg 
      aagataaaa atcaaagtt ttgaagaca cttttgttc 
      ttccctggg acgtttatt tttgaaaaa gtcaccaga 
      agcaaactt gtacaaaac ataacagca accttgaac 
      accgaaggt gttaaaaaa ttggctgga atttaccgg 
      gacgtaatt ttagccaca aacgataaa gttattaat 
      gaaaattcg acggaaaag tggtggaat gttgatagg 
      atttatgcg tctcattta ttggtcaaa ttattaaat 
      cacccctcc atgcacgaa aacaacgac tggaaggtc 
      gaccaatta gtttttcta atgaaattg gggcttttg 
      aaggacgat aacatcggt aacgacttg gccgactcg 
      tttagagaa accttctac tcgtcactt gatttaaaa 
      ttatcaaaa ctggacgat ttgaggcaa attttaagc 
      aaaatcgcc caagagttt gacgcactc gctacgctc 
      gtacgtcaa ccgttgagt gacgaaacg gccgaaatg 
      tggcaacgc acgcttgac gtagccacg aaattgcaa 
      aactcatcg aagaagaaa aattcggtc tttcacaca 
      ctctttctc catcttggg cttcaattg ttcaatggt 
      aataacgcg aagttggcg tctgattcg ctccaagag 
      cttatcatt tgttacgat aaaacgaaa aaaaagagc 
      gtcgatgaa agcgagctg gcttggatc gaagtcgtt 
      gttgattta ttcctaaat ttgttatcg cacaattcg 
      catttgttg cgcagtgtg ataagttgt gtctttccg 
      cacttgtgt aaatacatg actgggagc gcgattcac 
      cagattttg ggcgttttg gatccgaaa agtgattcg 
      aatccgttg agtcgaatc gatgagagt agtagcgac 
      gaagaggtg gaggaggag gaggaagat gaggacgaa 
      gaagaggaa gaaataagt gacgaagaa gaggaagaa 
      ccggttaat gataaactg cgaatggcg ttacatcaa 
      gcgttgggt gcaagtgtt gattcggaa gccgaaagt 
      gtagatctt gacgatttg gacgatgaa gaaggggaa 
      aaattagac gaagctttg agccaagca ttcaagcaa 
      tttaaaccg aacttgggg aggagaaaa aagcagaag 
      aaggacgaa gaagccttg actcatttc cggattcgg 
      gttttggac ttgatcgag atttatcta aattcaaat 
      ccgaccatg atcctcact ttggagata atgttgcct 
      ttgctccaa tgtctcgag ttttgcatc agagacgac 
      caccaaaag cccctccaa gacaggcta aaatcctgt 
      ttgaagaaa ctctcttcg ttgaagaaa ttcgacgac 
      tgtgagggc gtgaatgag gaggttttg gtcgaactg 
      ctcaaaagt ctcctcgag aaagggacg cgaaacgta 
      attatcatc caagaaatg gccgaaaaa ctcgccgaa 
      tgctgcatt ttcgtaatc aattgttcc gaatttgtc 
      cctaagaag aagaagatc aaagccgtg ataaaggac 
      ggtcttgag gcgtttttc acgcgccgt gattgcgtg 
      attccttac gtcctgttc aaaaattta gtccaaacc 
      aactgggag ggggtgctg gacttggtg ccccaattg 
      ttcggtttt gttttcgca aattccatt cggcccttc 
      cgtcgtggt caagccgtc gagcttttg cggcttttc 
      ttcagcaat catcgattt ttctcgacg cattgtgaa 
      caagtcaag tcgattgaa gaccacact aggaatttt 
      ctccgtgat tgtgtcgat ttcttccgc gagttttac 
      gcgaatccg actgagagg aaagtgaag gagaagttt 
      ttggcgtcg ctgtttgag cttttggcg caattgaag 
      agttacgag tttgagagt tgggcagtt ttgggtgat 
      gcggtcagg gattgtcgc tcgatttta acgttttcg 
      aaggatacg aaagttgcg ttcaataaa ttgtgcagt 
      agtttgaag atttccaat gttgtggaa atgaagcag 
      aagacggtg aagttgaat acggtcgag gagtgtgaa 
      agtgaggag aaggaggag aagaggagg aagaagaag 
      ttgagtaag gataagttg aagttgaag aaggagtcc 
      aaggagttg aggttgcaa agcttgtcg gaagggtta 
      aagaggaag tttgagtgg gaggaaggt gaagaagag 
      gcgaagaaa gcgaaaact gatgatttt taa
    mRNA sequence
    • >TC015701 RA
      ctcaattcg cgtcatggc gggcgaaga gcacgtgga 
      aattgtgga aaacggtga aaatcatca aaaaacaaa 
      taaaagaaa acgtgaatc gtccgtttt ggacaattt 
      caccaaatt aacaaacgg gcaagagaa agtgcgggt 
      taaagccgg cattgattt aatccggca tttaacaag 
      tgacaaaaa cggggaaca aagtaacga tgagttgaa 
      atacgcact agggcgttt gatacgtgg cctcggctc 
      gtctaaaat tcacgcaaa aactggctt ttttgccgc 
      tttggttgc cttacttaa ccttaaaac tatcacgat 
      tgaggagat tttcacaca cgtggagaa ggaattgca 
      caaaggggg cagcaacag taaaagtga aaatgccga 
      tatttgttc aggacagat tttgctctg tggggctat 
      tcttaggtc taatttgta cgaaagctg tgacgaaaa 
      agaaaaaat taaagtttt ggaaatgtt gctcaaggc 
      cgggaaaga aagatcata cttgaatct agcctcctg 
      taaattttt gaccgattt atttcagca ggttgagca 
      aaaagaatt cgaaaccgt gatttttcc actcattaa 
      aaacgattt agcgaaacc gtggcaaga ccaaaactt 
      ggactcact ttttttgtt tctacaaat acaaaatca 
      ttttcccgg ctttttaca tgcgaaatt cttgaaaaa 
      taatttagg gaccgaaga attaatttg tagcgaaag 
      tttgtacga cttggccct gactttgac ttctattcc 
      acgagtatc gtccttaaa tcacccaat ctttgactt 
      gtttatcaa aaccttatc acagacaga cttaacagt 
      tagtttttt caacgaatt agatcgcac cttgattag 
      gcaaaaccg taacaaatt tttcgtcgc aactaaact 
      cttgaccgg aattttgga cgaacttaa cgatttttc 
      cctaattcc cgaactttt gacacgaaa ttacctcca 
      acaattgct aagcactta caagacggc tgttggaaa 
      acaaaaaga taaagaatt ccaaaatct gacccaaaa 
      attattcga aacactcgt cagttgttt gaaaaaaga 
      cggagtcaa gtcgaagat aaaaatcaa agttttgaa 
      gacactttt gttcttccc tgggacgtt tatttttga 
      aaaagtcac cagaagcaa acttgtaca aaacataac 
      agcaacctt gaacaccga aggtgttaa aaaattggc 
      tggaattta ccgggacgt aattttagc cacaaacga 
      taaagttat taatgaaaa ttcgacgga aaagtggtg 
      gaatgttga taggattta tgcgtctca tttattggt 
      caaattatt aaatcaccc ctccatgca cgaaaacaa 
      cgactggaa ggtcgacca attagtttt tctaatgaa 
      attggggct tttgaagga cgataacat cggtaacga 
      cttggccga ctcgtttag agaaacctt ctactcgtc 
      acttgattt aaaattatc aaaactgga cgatttgag 
      gcaaatttt aagcaaaat cgcccaaga gtttgacgc 
      actcgctac gctcgtacg tcaaccgtt gagtgacga 
      aacggccga aatgtggca acgcacgct tgacgtagc 
      cacgaaatt gcaaaactc atcgaagaa gaaaaattc 
      ggtctttca cacactctt tctccatct tgggcttca 
      attgttcaa tggtaataa cgcgaagtt ggcgtctga 
      ttcgctcca agagcttat catttgtta cgataaaac 
      gaaaaaaaa gagcgtcga tgaaagcga gctggcttg 
      gatcgaagt cgttgttga tttattcct aaatttgtt 
      atcgcacaa ttcgcattt gttgcgcag tgtgataag 
      ttgtgtctt tccgcactt gtgtaaata catgactgg 
      gagcgcgat tcaccagat tttgggcgt tttggatcc 
      gaaaagtga ttcgaatcc gttgagtcg aatcgatga 
      gagtagtag cgacgaaga ggtggagga ggaggagga 
      agatgagga cgaagaaga ggaagaaat aagtgacga 
      agaagagga agaaccggt taatgataa actgcgaat 
      ggcgttaca tcaagcgtt gggtgcaag tgttgattc 
      ggaagccga aagtgtaga tcttgacga tttggacga 
      tgaagaagg ggaaaaatt agacgaagc tttgagcca 
      agcattcaa gcaatttaa accgaactt ggggaggag 
      aaaaaagca gaagaagga cgaagaagc cttgactca 
      tttccggat tcgggtttt ggacttgat cgagattta 
      tctaaattc aaatccgac catgatcct cactttgga 
      gataatgtt gcctttgct ccaatgtct cgagttttg 
      catcagaga cgaccacca aaagcccct ccaagacag 
      gctaaaatc ctgtttgaa gaaactctc ttcgttgaa 
      gaaattcga cgactgtga gggcgtgaa tgaggaggt 
      tttggtcga actgctcaa aagtctcct cgagaaagg 
      gacgcgaaa cgtaattat catccaaga aatggccga 
      aaaactcgc cgaatgctg cattttcgt aatcaattg 
      ttccgaatt tgtccctaa gaagaagaa gatcaaagc 
      cgtgataaa ggacggtct tgaggcgtt tttcacgcg 
      ccgtgattg cgtgattcc ttacgtcct gttcaaaaa 
      tttagtcca aaccaactg ggagggggt gctggactt 
      ggtgcccca attgttcgg ttttgtttt cgcaaattc 
      cattcggcc cttccgtcg tggtcaagc cgtcgagct 
      tttgcggct tttcttcag caatcatcg atttttctc 
      gacgcattg tgaacaagt caagtcgat tgaagacca 
      cactaggaa ttttctccg tgattgtgt cgatttctt 
      ccgcgagtt ttacgcgaa tccgactga gaggaaagt 
      gaaggagaa gtttttggc gtcgctgtt tgagctttt 
      ggcgcaatt gaagagtta cgagtttga gagttgggc 
      agttttggg tgatgcggt cagggattg tcgctcgat 
      tttaacgtt ttcgaagga tacgaaagt tgcgttcaa 
      taaattgtg cagtagttt gaagatttc caatgttgt 
      ggaaatgaa gcagaagac ggtgaagtt gaatacggt 
      cgaggagtg tgaaagtga ggagaagga ggagaagag 
      gaggaagaa gaagttgag taaggataa gttgaagtt 
      gaagaagga gtccaagga gttgaggtt gcaaagctt 
      gtcggaagg gttaaagag gaagtttga gtgggagga 
      aggtgaaga agaggcgaa gaaagcgaa aactgatga 
      tttttaaat aaattattt ttgaagacg ctttctggt 
      tttaacgca ttttccaaa cctatttgt tcccttctt 
      catttgaaa ttatgaaaa tgttccggt ttttttatt 
      ttattccca tccaagaaa aaggtcaaa agtaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY