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Gene information
General information
TC003127 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1368598..1373785
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003127
    iBeetle screen iB_11045

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003127 RA
      MFLLYNRTH EHSRERERE RDRDRERER DRERERERE 
      RERERERER DREIRDRER EREEKKSTK DLLKEKEME 
      EEAKEKKKA ERRAREKEA AYQERLRAW ETRERRKAK 
      EYEKEREKE RLKEEERER EAKRLKEFL EDYDDERDD 
      IKYYKGREL QRRLAERVK EAELDTRDR QKEKEEIEE 
      LKNRIFSGD HEDPNAEFE RVKREKEEQ YRPRLLIDV 
      NLDHWKQKE KEKERELER QKARERDRY REVERQRDR 
      QRAREEREK YVLQQAAQE LAAVEAEPI DSDSDMDEN 
      HYSPAPPEP DGNSNDDSE STKWTQVQV GEDDSRHSF 
      TSNHDENPP PPTGIGMKI TLAKPITFS PSTQQPKRK 
      KLDLREVFN PDEDDSNTQ VKKRKLVPL DYNEDKKGK 
      KDKDGEKKS EESLKSQEE KKKTIKSLI EKIPTEKEA 
      LFAYQLDWS AIDNSLMER KIRPWINKK IIEYIGEPE 
      PTLVDFICS KVLAGSQPQ VILQDVQMV LDEEAEVFV 
      VKMWRLIIY EVEAKKLGL VK
    • >TC003127 RB
      MSYPGRPPI MQGLPMAYM PMGGPAPPL MPAVMPLQH 
      MVPIQVTSA PQIRALPRL NASREQLKQ LQGPAVTVF 
      VGNITERAP DAMIRQILA ACGHVMSWK KVKAFGFCE 
      LAGPDAGLR AVRLLHDLM VGDKRLVAK VDAKTKTVL 
      DEYKEARRR KKQKGSSPL QDEPEEDVL DEETQALDA 
      VAVERIKQI LNEYEDEMN NFELKKEEE VIEKCHKVL 
      EEADVEEEK RDLITREIG KFREIMKKQ EEEKEVSKR 
      KKEEKEKNR EKSPSHSPS PRRRDKDDK RRRRSRSKS 
      RTHEHSRER ERERDRDRE RERDRERER ERERERERE 
      RERDREIRD REREREEKK STKDLLKEK EMEEEAKEK 
      KKAERRARE KEAAYQERL RAWETRERR KAKEYEKER 
      EKERLKEEE REREAKRLK EFLEDYDDE RDDIKYYKG 
      RELQRRLAE RVKEAELDT RDRQKEKEE IEELKNRIF 
      SGDHEDPNA EFERVKREK EEQYRPRLL IDVNLDHWK 
      QKEKEKERE LERQKARER DRYREVERQ RDRQRAREE 
      REKYVLQQA AQELAAVEA EPIDSDSDM DENHYSPAP 
      PEPDGNSND DSESTKWTQ VQVGEDDSR HSFTSNHDE 
      NPPPPTGIG MKITLAKPI TFSPSTQQP KRKKLDLRE 
      VFNPDEDDS NTQVKKRKL VPLDYNEDK KGKKDKDGE 
      KKSEESLKS QEEKKKTIK SLIEKIPTE KEALFAYQL 
      DWSAIDNSL MERKIRPWI NKKIIEYIG EPEPTLVDF 
      ICSKVLAGS QPQVILQDV QMVLDEEAE VFVVKMWRL 
      IIYEVEAKK LGLVK
    • >TC003127 RC
      MSYPGRPPI MQGLPMAYM PMGGPAPPL MPAVMPLQH 
      MVPIQVTSA PQIRALPRL NASREQLKQ LQGPAVTVF 
      VGNITERAP DAMIRQILA ACGHVMSWK KVKAFGFCE 
      LAGPDAGLR AVRLLHDLM VGDKRLVAK VDAKTKTVL 
      DEYKEARRR KKQKGSSPL QDEPEEDVL DEETQALDA 
      VAVERIKQI LNEYEDEMN NFELKKEEE VIEKCHKVL 
      EEADVEEEK RDLITREIG KFREIMKVS ITFFNKNGG 
      QFV
    CDS sequence
    • >TC003127 RA
      atgtttttg ctttataac aggacgcat gaacatagc 
      agggaacgc gagagggaa cgggataga gatagggaa 
      cgagaaagg gatcgcgaa cgcgagcga gaaagagaa 
      cgagaacgt gaacgcgaa cgggagaga gatcgtgaa 
      atcagggat cgcgaacgc gagcgagaa gaaaaaaag 
      agcactaaa gatttacta aaggagaag gagatggaa 
      gaagaagcg aaagaaaag aaaaaagct gagagaaga 
      gccagggaa aaggaagca gcttatcag gaaagactc 
      cgcgcttgg gagactcgc gaacgccga aaagccaag 
      gaatatgag aaagaaagg gaaaaggaa agactgaag 
      gaggaggaa cgcgaacgt gaagccaag agattgaaa 
      gagtttctg gaggattat gacgacgaa cgcgacgat 
      atcaaatat tacaagggc agggagctt caaaggagg 
      ctggctgaa cgggtcaaa gaggcagaa cttgatact 
      agagacagg cagaaggag aaagaggaa attgaagaa 
      ttgaagaac aggatattt tcaggtgac cacgaagat 
      cccaacgct gaattcgaa agagtgaaa cgagaaaag 
      gaggaacaa tacaggccc agattattg attgacgtt 
      aatttggat cactggaag cagaaagaa aaggaaaaa 
      gaacgggag ctggaaaga caaaaggcg agagaacga 
      gaccgttat cgtgaagtt gagaggcaa agggacaga 
      cagagggcg agggaagaa agggaaaaa tatgtatta 
      caacaagca gcgcaagaa ttggctgcg gttgaagca 
      gagcccatt gacagtgat agtgacatg gacgaaaat 
      cactacagt ccagcgccg ccagaacct gatggtaac 
      tcaaacgac gattcagaa tcgactaaa tggactcag 
      gtgcaggtt ggtgaggac gattctaga cactctttc 
      acctcaaat cacgacgaa aacccgcct cctccaacc 
      ggaatcggg atgaaaatt actttagct aaaccaatt 
      acctttagt ccttcaaca caacaaccg aaacgaaag 
      aagcttgat cttcgggaa gtgttcaat cctgatgaa 
      gacgacagt aatacacaa gtgaaaaaa cgaaaactt 
      gtcccactt gattacaac gaagacaag aaaggtaag 
      aaggacaaa gacggtgag aaaaaatca gaagagtcg 
      ttgaagtca caagaggaa aagaaaaag accataaag 
      tcattaatc gagaaaata ccgacagag aaggaggcg 
      ttatttgcg tatcagctt gactggtca gctatcgac 
      aatagtttg atggaacga aagattcgt ccgtggatt 
      aacaagaaa attatagag tacattggg gagcctgag 
      cccactttg gttgatttt atctgttcc aaagtgttg 
      gcaggctcc cagcctcaa gtcatcttg caggatgtt 
      caaatggta ttggatgaa gaagccgaa gtttttgtt 
      gttaaaatg tggaggttg ataatttat gaagtggaa 
      gcaaagaag ctaggttta gtgaagtag
    • >TC003127 RB
      atgtcgtat ccaggccgc cccccgatc atgcagggc 
      cttccgatg gcgtacatg cccatgggg ggccccgcg 
      cccccgctt atgcccgcc gttatgccc ctccaacat 
      atggtacct attcaggta acaagcgcc ccgcaaatc 
      cgggctttg ccgcgactg aacgccagc cgggagcag 
      ctgaagcaa ctgcagggg cctgcggtg acggtgttt 
      gttggtaat attacggaa agagccccc gatgcaatg 
      attcgtcaa attttggcc gcgtgcgga cacgtcatg 
      agctggaag aaggtaaaa gcatttggt ttttgtgaa 
      ctagctggg cctgacgcc ggcttaagg gctgttagg 
      ttgcttcat gatctcatg gttggggac aagaggctg 
      gtggcgaag gtcgacgct aagacaaag actgtgcta 
      gatgaatat aaagaagct cggcgccgt aaaaagcaa 
      aaggggtcg tcacctctt caagacgaa cctgaagaa 
      gatgtcttg gacgaggag acccaagcg ttggacgct 
      gtggccgtc gaaagaatt aaacaaatc ctcaacgaa 
      tatgaagat gaaatgaac aattttgaa ttaaagaaa 
      gaagaggaa gtgatcgag aagtgtcat aaagtatta 
      gaagaggcc gatgttgag gaagaaaaa cgtgatttg 
      atcacaaga gagattgga aagtttcgc gaaatcatg 
      aaaaaacaa gaggaagag aaggaagtc agcaagcgg 
      aaaaaagaa gaaaaggag aagaacaga gagaagtca 
      ccctcacat tcgcccagt ccgcgaaga cgtgataaa 
      gatgacaaa aggagacgt cgttcaaga tctaagtca 
      aggacgcat gaacatagc agggaacgc gagagggaa 
      cgggataga gatagggaa cgagaaagg gatcgcgaa 
      cgcgagcga gaaagagaa cgagaacgt gaacgcgaa 
      cgggagaga gatcgtgaa atcagggat cgcgaacgc 
      gagcgagaa gaaaaaaag agcactaaa gatttacta 
      aaggagaag gagatggaa gaagaagcg aaagaaaag 
      aaaaaagct gagagaaga gccagggaa aaggaagca 
      gcttatcag gaaagactc cgcgcttgg gagactcgc 
      gaacgccga aaagccaag gaatatgag aaagaaagg 
      gaaaaggaa agactgaag gaggaggaa cgcgaacgt 
      gaagccaag agattgaaa gagtttctg gaggattat 
      gacgacgaa cgcgacgat atcaaatat tacaagggc 
      agggagctt caaaggagg ctggctgaa cgggtcaaa 
      gaggcagaa cttgatact agagacagg cagaaggag 
      aaagaggaa attgaagaa ttgaagaac aggatattt 
      tcaggtgac cacgaagat cccaacgct gaattcgaa 
      agagtgaaa cgagaaaag gaggaacaa tacaggccc 
      agattattg attgacgtt aatttggat cactggaag 
      cagaaagaa aaggaaaaa gaacgggag ctggaaaga 
      caaaaggcg agagaacga gaccgttat cgtgaagtt 
      gagaggcaa agggacaga cagagggcg agggaagaa 
      agggaaaaa tatgtatta caacaagca gcgcaagaa 
      ttggctgcg gttgaagca gagcccatt gacagtgat 
      agtgacatg gacgaaaat cactacagt ccagcgccg 
      ccagaacct gatggtaac tcaaacgac gattcagaa 
      tcgactaaa tggactcag gtgcaggtt ggtgaggac 
      gattctaga cactctttc acctcaaat cacgacgaa 
      aacccgcct cctccaacc ggaatcggg atgaaaatt 
      actttagct aaaccaatt acctttagt ccttcaaca 
      caacaaccg aaacgaaag aagcttgat cttcgggaa 
      gtgttcaat cctgatgaa gacgacagt aatacacaa 
      gtgaaaaaa cgaaaactt gtcccactt gattacaac 
      gaagacaag aaaggtaag aaggacaaa gacggtgag 
      aaaaaatca gaagagtcg ttgaagtca caagaggaa 
      aagaaaaag accataaag tcattaatc gagaaaata 
      ccgacagag aaggaggcg ttatttgcg tatcagctt 
      gactggtca gctatcgac aatagtttg atggaacga 
      aagattcgt ccgtggatt aacaagaaa attatagag 
      tacattggg gagcctgag cccactttg gttgatttt 
      atctgttcc aaagtgttg gcaggctcc cagcctcaa 
      gtcatcttg caggatgtt caaatggta ttggatgaa 
      gaagccgaa gtttttgtt gttaaaatg tggaggttg 
      ataatttat gaagtggaa gcaaagaag ctaggttta 
      gtgaagtag
    • >TC003127 RC
      atgtcgtat ccaggccgc cccccgatc atgcagggc 
      cttccgatg gcgtacatg cccatgggg ggccccgcg 
      cccccgctt atgcccgcc gttatgccc ctccaacat 
      atggtacct attcaggta acaagcgcc ccgcaaatc 
      cgggctttg ccgcgactg aacgccagc cgggagcag 
      ctgaagcaa ctgcagggg cctgcggtg acggtgttt 
      gttggtaat attacggaa agagccccc gatgcaatg 
      attcgtcaa attttggcc gcgtgcgga cacgtcatg 
      agctggaag aaggtaaaa gcatttggt ttttgtgaa 
      ctagctggg cctgacgcc ggcttaagg gctgttagg 
      ttgcttcat gatctcatg gttggggac aagaggctg 
      gtggcgaag gtcgacgct aagacaaag actgtgcta 
      gatgaatat aaagaagct cggcgccgt aaaaagcaa 
      aaggggtcg tcacctctt caagacgaa cctgaagaa 
      gatgtcttg gacgaggag acccaagcg ttggacgct 
      gtggccgtc gaaagaatt aaacaaatc ctcaacgaa 
      tatgaagat gaaatgaac aattttgaa ttaaagaaa 
      gaagaggaa gtgatcgag aagtgtcat aaagtatta 
      gaagaggcc gatgttgag gaagaaaaa cgtgatttg 
      atcacaaga gagattgga aagtttcgc gaaatcatg 
      aaagtaagt ataactttt ttcaacaaa aacgggggt 
      caatttgtt taa
    mRNA sequence
    • >TC003127 RA
      taccatgtt tatgaattt ttaaaagaa ctttgaaat 
      atcccaaaa taattatca tctaaagaa aagaattaa 
      catcgggga agaataatt cgtgggtca gttttagaa 
      tattttcga atgcttgta aataatatt gattcatag 
      attaacatg acagcaatc ccagtctaa atcccgagt 
      cactaacct gtttaaaaa tttctaact cgttacagc 
      attgaatga aattgccaa caataaaca aaaacgata 
      aaactgaac gagctttaa tatttaaaa ccagtaacg 
      tatacctga tcatcactg gtatgagaa atatttttt 
      taataaaaa ccatcaaac ataatagcg aggtgatta 
      attggcgtt tgtttttat tttagaaac aagaggaag 
      agaaggaag tcagcaagc ggaaaaaag aagaaaagg 
      agaagaaca gagagaagt caccctcac attcgccca 
      gtccgcgaa gacgtgata aagatgaca aaaggagac 
      gtcgttcaa gatctaagt caaggttgc ttgtttttt 
      gcattcgtt gccgataat aatgttttt gctttataa 
      caggacgca tgaacatag cagggaacg cgagaggga 
      acgggatag agataggga acgagaaag ggatcgcga 
      acgcgagcg agaaagaga acgagaacg tgaacgcga 
      acgggagag agatcgtga aatcaggga tcgcgaacg 
      cgagcgaga agaaaaaaa gagcactaa agatttact 
      aaaggagaa ggagatgga agaagaagc gaaagaaaa 
      gaaaaaagc tgagagaag agccaggga aaaggaagc 
      agcttatca ggaaagact ccgcgcttg ggagactcg 
      cgaacgccg aaaagccaa ggaatatga gaaagaaag 
      ggaaaagga aagactgaa ggaggagga acgcgaacg 
      tgaagccaa gagattgaa agagtttct ggaggatta 
      tgacgacga acgcgacga tatcaaata ttacaaggg 
      cagggagct tcaaaggag gctggctga acgggtcaa 
      agaggcaga acttgatac tagagacag gcagaagga 
      gaaagagga aattgaaga attgaagaa caggatatt 
      ttcaggtga ccacgaaga tcccaacgc tgaattcga 
      aagagtgaa acgagaaaa ggaggaaca atacaggcc 
      cagattatt gattgacgt taatttgga tcactggaa 
      gcagaaaga aaaggaaaa agaacggga gctggaaag 
      acaaaaggc gagagaacg agaccgtta tcgtgaagt 
      tgagaggca aagggacag acagagggc gagggaaga 
      aagggaaaa atatgtatt acaacaagc agcgcaaga 
      attggctgc ggttgaagc agagcccat tgacagtga 
      tagtgacat ggacgaaaa tcactacag tccagcgcc 
      gccagaacc tgatggtaa ctcaaacga cgattcaga 
      atcgactaa atggactca ggtgcaggt tggtgagga 
      cgattctag acactcttt cacctcaaa tcacgacga 
      aaacccgcc tcctccaac cggaatcgg gatgaaaat 
      tactttagc taaaccaat tacctttag tccttcaac 
      acaacaacc gaaacgaaa gaagcttga tcttcggga 
      agtgttcaa tcctgatga agacgacag taatacaca 
      agtgaaaaa acgaaaact tgtcccact tgattacaa 
      cgaagacaa gaaaggtaa gaaggacaa agacggtga 
      gaaaaaatc agaagagtc gttgaagtc acaagagga 
      aaagaaaaa gaccataaa gtcattaat cgagaaaat 
      accgacaga gaaggaggc gttatttgc gtatcagct 
      tgactggtc agctatcga caatagttt gatggaacg 
      aaagattcg tccgtggat taacaagaa aattataga 
      gtacattgg ggagcctga gcccacttt ggttgattt 
      tatctgttc caaagtgtt ggcaggctc ccagcctca 
      agtcatctt gcaggatgt tcaaatggt attggatga 
      agaagccga agtttttgt tgttaaaat gtggaggtt 
      gataattta tgaagtgga agcaaagaa gctaggttt 
      agtgaagta gcagttatt tcaaatgta tataatgag 
      atggacatt aggtttaca tttatataa ttcatgtac 
      atcaattta tcaagtata taatttgct gcaggtatt 
      taattcaat aattgtgtt tatctgaat gggtcagga 
      tatgtcacg ttcgttgaa tcactgaga aaaagtttt 
      attttatgt aagcatgtc caagatttt aaaaaataa 
      aattaattt tcaagc
    • >TC003127 RB
      accttctgt agttgagtg acatgtgag attcggtcg 
      ttagttttt taccgaaat tatcaaacc gagcgtact 
      tgtgtttgc caatctacc gcagaaata atacattag 
      ttacataga atccaaata atacttggt gttttgcaa 
      aaaacgccc gagaagcag gcactcttt aggtcgaac 
      ctcccctca tctgatgtc ctgtttggc aagttgatc 
      gtgcccgtt aacttccac aatgtcgta tccaggccg 
      ccccccgat catgcaggg ccttccgat ggcgtacat 
      gcccatggg gggccccgc gcccccgct tatgcccgc 
      cgttatgcc cctccaaca tatggtacc tattcaggt 
      aacaagcgc cccgcaaat ccgggcttt gccgcgact 
      gaacgccag ccgggagca gctgaagca actgcaggg 
      gcctgcggt gacggtgtt tgttggtaa tattacgga 
      aagagcccc cgatgcaat gattcgtca aattttggc 
      cgcgtgcgg acacgtcat gagctggaa gaaggtaaa 
      agcatttgg tttttgtga actagctgg gcctgacgc 
      cggcttaag ggctgttag gttgcttca tgatctcat 
      ggttgggga caagaggct ggtggcgaa ggtcgacgc 
      taagacaaa gactgtgct agatgaata taaagaagc 
      tcggcgccg taaaaagca aaaggggtc gtcacctct 
      tcaagacga acctgaaga agatgtctt ggacgagga 
      gacccaagc gttggacgc tgtggccgt cgaaagaat 
      taaacaaat cctcaacga atatgaaga tgaaatgaa 
      caattttga attaaagaa agaagagga agtgatcga 
      gaagtgtca taaagtatt agaagaggc cgatgttga 
      ggaagaaaa acgtgattt gatcacaag agagattgg 
      aaagtttcg cgaaatcat gaaaaaaca agaggaaga 
      gaaggaagt cagcaagcg gaaaaaaga agaaaagga 
      gaagaacag agagaagtc accctcaca ttcgcccag 
      tccgcgaag acgtgataa agatgacaa aaggagacg 
      tcgttcaag atctaagtc aaggacgca tgaacatag 
      cagggaacg cgagaggga acgggatag agataggga 
      acgagaaag ggatcgcga acgcgagcg agaaagaga 
      acgagaacg tgaacgcga acgggagag agatcgtga 
      aatcaggga tcgcgaacg cgagcgaga agaaaaaaa 
      gagcactaa agatttact aaaggagaa ggagatgga 
      agaagaagc gaaagaaaa gaaaaaagc tgagagaag 
      agccaggga aaaggaagc agcttatca ggaaagact 
      ccgcgcttg ggagactcg cgaacgccg aaaagccaa 
      ggaatatga gaaagaaag ggaaaagga aagactgaa 
      ggaggagga acgcgaacg tgaagccaa gagattgaa 
      agagtttct ggaggatta tgacgacga acgcgacga 
      tatcaaata ttacaaggg cagggagct tcaaaggag 
      gctggctga acgggtcaa agaggcaga acttgatac 
      tagagacag gcagaagga gaaagagga aattgaaga 
      attgaagaa caggatatt ttcaggtga ccacgaaga 
      tcccaacgc tgaattcga aagagtgaa acgagaaaa 
      ggaggaaca atacaggcc cagattatt gattgacgt 
      taatttgga tcactggaa gcagaaaga aaaggaaaa 
      agaacggga gctggaaag acaaaaggc gagagaacg 
      agaccgtta tcgtgaagt tgagaggca aagggacag 
      acagagggc gagggaaga aagggaaaa atatgtatt 
      acaacaagc agcgcaaga attggctgc ggttgaagc 
      agagcccat tgacagtga tagtgacat ggacgaaaa 
      tcactacag tccagcgcc gccagaacc tgatggtaa 
      ctcaaacga cgattcaga atcgactaa atggactca 
      ggtgcaggt tggtgagga cgattctag acactcttt 
      cacctcaaa tcacgacga aaacccgcc tcctccaac 
      cggaatcgg gatgaaaat tactttagc taaaccaat 
      tacctttag tccttcaac acaacaacc gaaacgaaa 
      gaagcttga tcttcggga agtgttcaa tcctgatga 
      agacgacag taatacaca agtgaaaaa acgaaaact 
      tgtcccact tgattacaa cgaagacaa gaaaggtaa 
      gaaggacaa agacggtga gaaaaaatc agaagagtc 
      gttgaagtc acaagagga aaagaaaaa gaccataaa 
      gtcattaat cgagaaaat accgacaga gaaggaggc 
      gttatttgc gtatcagct tgactggtc agctatcga 
      caatagttt gatggaacg aaagattcg tccgtggat 
      taacaagaa aattataga gtacattgg ggagcctga 
      gcccacttt ggttgattt tatctgttc caaagtgtt 
      ggcaggctc ccagcctca agtcatctt gcaggatgt 
      tcaaatggt attggatga agaagccga agtttttgt 
      tgttaaaat gtggaggtt gataattta tgaagtgga 
      agcaaagaa gctaggttt agtgaagta gcagttatt 
      tcaaatgta tataatgag atggacatt aggtttaca 
      tttatataa ttcatgtac atcaattta tcaagtata 
      taatttgct gcaggtatt taattcaat aattgtgtt 
      tatctgaat gggtcagga tatgtcacg ttcgttgaa 
      tcactgaga aaaagtttt attttatgt aagcatgtc 
      caagatttt aaaaaataa aattaattt tcaagc
    • >TC003127 RC
      accttctgt agttgagtg acatgtgag attcggtcg 
      ttagttttt taccgaaat tatcaaacc gagcgtact 
      tgtgtttgc caatctacc gcagaaata atacattag 
      ttacataga atccaaata atacttggt gttttgcaa 
      aaaacgccc gagaagcag gcactcttt aggtcgaac 
      ctcccctca tctgatgtc ctgtttggc aagttgatc 
      gtgcccgtt aacttccac aatgtcgta tccaggccg 
      ccccccgat catgcaggg ccttccgat ggcgtacat 
      gcccatggg gggccccgc gcccccgct tatgcccgc 
      cgttatgcc cctccaaca tatggtacc tattcaggt 
      aacaagcgc cccgcaaat ccgggcttt gccgcgact 
      gaacgccag ccgggagca gctgaagca actgcaggg 
      gcctgcggt gacggtgtt tgttggtaa tattacgga 
      aagagcccc cgatgcaat gattcgtca aattttggc 
      cgcgtgcgg acacgtcat gagctggaa gaaggtaaa 
      agcatttgg tttttgtga actagctgg gcctgacgc 
      cggcttaag ggctgttag gttgcttca tgatctcat 
      ggttgggga caagaggct ggtggcgaa ggtcgacgc 
      taagacaaa gactgtgct agatgaata taaagaagc 
      tcggcgccg taaaaagca aaaggggtc gtcacctct 
      tcaagacga acctgaaga agatgtctt ggacgagga 
      gacccaagc gttggacgc tgtggccgt cgaaagaat 
      taaacaaat cctcaacga atatgaaga tgaaatgaa 
      caattttga attaaagaa agaagagga agtgatcga 
      gaagtgtca taaagtatt agaagaggc cgatgttga 
      ggaagaaaa acgtgattt gatcacaag agagattgg 
      aaagtttcg cgaaatcat gaaagtaag tataacttt 
      tttcaacaa aaacggggg tcaatttgt ttaaaatcg 
      gtctgacgg aaagtatac atgactttc agttgtttc 
      tattcaaaa ttaaagtca gggcggggg gtttgagaa 
      ttttctaaa aagtattag aagtaaggt tgcgctcat 
      gtggttctt gaaaccaaa caggcaaat ggaagcaac 
      gcagtccca cctaggttc ggcccatgg cgccgttgg 
      tgagtaacg tgtcaacag ttatctcca ctgtacctt 
      tttaaattc acacacata ttttttcaa tttcaataa 
      tagtctcat ttctgatag aattaatcc aaatatgtg 
      tgcgagctt taaaacatg atttttctc ctgttattg 
      ttaatacgt aaatggtta caattgcct tgcttcatt 
      tatttggtc caatttctt tcacagttt gcgaaagtg 
      atagtaacc atttatttg acatttttt gtccttttt 
      actagtcca ctttttggt atattagtt agtttttgt 
      gccacgggc tgtatttgt aagttatag tccgtggtg 
      taatatgtt tcgtcgaaa ttttggaga ttcgcaagt 
      tggggacgt atcaagttc agtaaggat ttatgtcgt 
      ctgttacaa gagtaagtt aaaacagta catgtagaa 
      atgtcttca cattacttt taagtaatt cattcgaaa 
      atatttttt acttttgtt ttattgttc ccacagggt 
      gtatggact tattgattt tttattatc taaaggtat 
      aatttacaa acgaaaatc atttctttg tattctgaa 
      gcagtaacc aattatttg attgttttt ttattctgt 
      tcaatacga agaaacgag ttctctttg tttcagcga 
      ctgggatat ttctgaaaa tgtcgaatt gagtatcgt 
      gttaaaatt tttatttcc gcttaagtt tttcgtggt 
      tatatgacg agtaagaac ggtctaatt tatgtttat 
      ttgctgtac tttttacct gctattttt aatcacaag 
      aaataatgg gaattattc aattacgtt ccgaattct 
      attctgttt taccgggtc ctcacaacc gcttaacgc 
      tttgagtta gcgaattca gtttttata ttctaaaaa 
      gatagaatg gattgaaaa ttactattc ctcccaagt 
      ttatctccg attagaact tcgtagttt aattatagg 
      gtaataatt atggattta ccaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY