Status
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Gene information
General information
TC005306 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14570616..14573773
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005306
    Gene ontology

    GO terms for Tribolium

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      Status: {{ goTerm.status }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09430

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Adult morphology
    odoriferous glands slightly colour darker - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection



    abdominal odoriferous glands content fragmented - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection



    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_11044

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005306 RA
      MSCPWAKIA NVEPVNLQD IMSEEVARE LQAKEDKKY 
      AEMFGPPSK QDNAISSEV LNSLEDDTC ESDAAIARM 
      LQMQFDKEY DENLKRTEE KYNGASKVS ISFENYRRM 
      PHNFDFESD SEEEEIVDI RDRRDWDRF DTLQRQFDS 
      IPPCGYKMQ NGSMVTKHD VTMNGRKNA CKLLSFPPD 
      FQTGDGENF DLKLSNKVF NSLKRHSQN EESRRHKVR 
      DKKEDRATQ EFGLDEFTR LLIYKIIQQ EILDNVNGV 
      VSIGKEAVI LHADANANY TERPLPPEC AIKVFKTTL 
      SEFKQRDKY IKDDHRFKG RMGNQTARK TVHLWAEKE 
      MHNLKRLKN ANIPCPEVV VLKKHVLVM SFIGENNKP 
      APKLKDAIM DEADYIIAY DQVVEAMKT LFTEAELIH 
      ADLSEYNIL WHQRQCYFI DVSQAVLPS HENAFYFLM 
      RDCNNVINF FTKKKVPKV TSSEELFKS ITGYNYNEK 
      KDLLQLQET FKMKPHLVD KPGIEPIFD FDKGWEKSK 
      QNLETVKLV EAPLA
    CDS sequence
    • >TC005306 RA
      atgtcgtgt ccgtgggcc aaaatcgca aacgttgaa 
      cctgtcaac ttacaagac atcatgtct gaagaagtg 
      gcacgtgaa ttacaagca aaagaagac aagaaatat 
      gccgaaatg tttggtcct ccatcgaaa caagacaac 
      gcaatctcc tctgaggtg cttaactca cttgaagac 
      gacacttgt gaatcagat gctgcaata gctcgaatg 
      ctccaaatg cagtttgac aaggaatac gacgaaaat 
      ttgaaacgc acagaggag aagtataac ggagcttcg 
      aaagtttca atttcattt gaaaattat cgtcggatg 
      ccccacaat ttcgacttt gaaagtgac tctgaggaa 
      gaagaaatc gtcgatatt cgtgaccga cgcgactgg 
      gaccgtttt gacacactc caaagacag tttgattca 
      atcccccct tgtgggtac aaaatgcaa aacggcagc 
      atggtcacc aaacacgat gttacaatg aacggccga 
      aaaaacgcc tgtaaatta ctgtctttc ccacctgat 
      ttccagacc ggagacggg gagaatttt gacttgaaa 
      ctttccaat aaagtgttc aacagtttg aaacgtcat 
      tcacaaaac gaagaatct cgcagacac aaagtccgc 
      gataaaaaa gaagatcgc gcaacgcaa gaattcggt 
      ctcgacgag ttcacacgt ctcctcatc tacaaaata 
      atccaacag gaaatactc gataacgtg aacggtgtt 
      gttagcatt ggcaaagaa gcggtaatt cttcatgct 
      gatgccaat gccaattat acagaacgc cctcttcca 
      ccagaatgt gcgattaaa gtcttcaaa acaactctg 
      tctgagttt aaacaacga gataaatac atcaaagac 
      gatcacaga tttaaaggt cgcatgggg aatcagaca 
      gcgaggaaa accgttcac ttgtgggcg gagaaggaa 
      atgcacaat ttgaaacga ttgaaaaat gcgaatatt 
      ccatgtccc gaagtagta gtcttgaaa aagcacgtc 
      cttgttatg tcgtttatt ggagagaat aataaaccg 
      gcgcctaaa ttaaaagac gcgataatg gacgaagcc 
      gattatatt atagcctat gaccaagtt gttgaggca 
      atgaaaacg ctttttact gaagcagag ttgattcat 
      gctgacttg tctgaatat aatattttg tggcatcaa 
      agacagtgc tattttatt gatgtcagt caggcggtc 
      ttgccctcc catgaaaat gcgttttat tttcttatg 
      agggattgc aacaacgtt ataaatttt ttcacaaag 
      aaaaaagtg cccaaggtt acaagctca gaagaactg 
      ttcaaatcc ataacaggt tataactat aatgagaaa 
      aaagattta cttcagtta caagaaacg ttcaaaatg 
      aaacctcat ctcgttgac aaaccagga attgagcct 
      atttttgat tttgataaa ggatgggaa aaatcgaaa 
      caaaatttg gaaactgta aaattagtt gaagcaccg 
      ttagcttag
    mRNA sequence
    • >TC005306 RA
      agaatccta aattttttt tatgttttt ggggatttt 
      aaatgtcaa gttgccaag ttggtgtgt ctgtcactt 
      gtcaacaag tcttccacc tccacagag gaatacatc 
      aattttcat ttcattgca caataaatc aacataaat 
      tcgtaaatt ccgatattt ttcatttag tagtattaa 
      aaagttagt atcggaaga cttaaccag ccatgtcgt 
      gtccgtggg ccaaaatcg caaacgttg aacctgtca 
      acttacaag acatcatgt ctgaagaag tggcacgtg 
      aattacaag caaaagaag acaagaaat atgccgaaa 
      tgtttggtc ctccatcga aacaagaca acgcaatct 
      cctctgagg tgcttaact cacttgaag acgacactt 
      gtgaatcag atgctgcaa tagctcgaa tgctccaaa 
      tgcagtttg acaaggaat acgacgaaa atttgaaac 
      gcacagagg agaagtata acggagctt cgaaagttt 
      caatttcat ttgaaaatt atcgtcgga tgccccaca 
      atttcgact ttgaaagtg actctgagg aagaagaaa 
      tcgtcgata ttcgtgacc gacgcgact gggaccgtt 
      ttgacacac tccaaagac agtttgatt caatccccc 
      cttgtgggt acaaaatgc aaaacggca gcatggtca 
      ccaaacacg atgttacaa tgaacggcc gaaaaaacg 
      cctgtaaat tactgtctt tcccacctg atttccaga 
      ccggagacg gggagaatt ttgacttga aactttcca 
      ataaagtgt tcaacagtt tgaaacgtc attcacaaa 
      acgaagaat ctcgcagac acaaagtcc gcgataaaa 
      aagaagatc gcgcaacgc aagaattcg gtctcgacg 
      agttcacac gtctcctca tctacaaaa taatccaac 
      aggaaatac tcgataacg tgaacggtg ttgttagca 
      ttggcaaag aagcggtaa ttcttcatg ctgatgcca 
      atgccaatt atacagaac gccctcttc caccagaat 
      gtgcgatta aagtcttca aaacaactc tgtctgagt 
      ttaaacaac gagataaat acatcaaag acgatcaca 
      gatttaaag gtcgcatgg ggaatcaga cagcgagga 
      aaaccgttc acttgtggg cggagaagg aaatgcaca 
      atttgaaac gattgaaaa atgcgaata ttccatgtc 
      ccgaagtag tagtcttga aaaagcacg tccttgtta 
      tgtcgttta ttggagaga ataataaac cggcgccta 
      aattaaaag acgcgataa tggacgaag ccgattata 
      ttatagcct atgaccaag ttgttgagg caatgaaaa 
      cgcttttta ctgaagcag agttgattc atgctgact 
      tgtctgaat ataatattt tgtggcatc aaagacagt 
      gctatttta ttgatgtca gtcaggcgg tcttgccct 
      cccatgaaa atgcgtttt attttctta tgagggatt 
      gcaacaacg ttataaatt ttttcacaa agaaaaaag 
      tgcccaagg ttacaagct cagaagaac tgttcaaat 
      ccataacag gttataact ataatgaga aaaaagatt 
      tacttcagt tacaagaaa cgttcaaaa tgaaacctc 
      atctcgttg acaaaccag gaattgagc ctatttttg 
      attttgata aaggatggg aaaaatcga aacaaaatt 
      tggaaactg taaaattag ttgaagcac cgttagctt 
      agcttaata attaattta ttaaaaact tgttaattt 
      tatgttaaa tagacgatt taaaaaaat atttttggc 
      caaaaaaaa aattgattt attatgttt gttattcaa 
      tcaaaaaat aaacagtaa aatctcgta gtacttgaa 
      aacttattg caccttgaa gcacatttc atacgttgg 
      cggtttttc cggtatctt taacatgtc taaattatc 
      actttggaa ccactttat ctcctcttc agtcaaatc 
      ttccaactt ggagtaaaa atcttgatt tggtttagt 
      ctgatgagg tgcgccacc aaacacatt ttgttcctg 
      ctgttgcat tccaaaatt gccaaaaat actttgagt 
      atgttgaaa cgccgccgg tttattact cgttgtatc 
      cccaaactg ttgtgccgt aaattgatt tacactccc 
      aaaatgact agttgaggg gggctgttg tgctgcgac 
      aaaaggggg aggctgttg ttggaaaat attttgttg 
      gggctgcgt cgtattttg cggaaatgg tgccccaaa 
      cattgagtt actcgcttg tgttgaggc ttgtgcgaa 
      aatcgattt attttgaat gttttgttg ttctccaaa 
      aattggatt agggacctt attagcagc aaacacact 
      ctgtgtttg aaccccgcc gaaaattcc acttggttg 
      agttgtgtt accaaaagc gggtttttg ctgcatctg 
      ggagcctcc aaaaatcga attttgcgt ttgatttga 
      tggtggagt attgtagat attttttag tatgttaat 
      aacatcaag tgaggggtt ttgcaaggc tttgatttt 
      cgttattgc ctgttgtaa catcatttg caaattttg 
      tttgaattg ttcaatagt gccgttttt ggctgattc 
      gtaaaataa atatctgat ttcttccat actgtgatc 
      ttcaaaacc gggaaactg gggcttgtc acgaaatgg 
      ggaatatga cgataagag ccattggcc acccttttc 
      tgcacaagt catatcatt cactactgc tttcaccaa 
      agtgtttgt atcaacagc agttgacgc attctgggg 
      tgctggttt gtttgcgta attttggac taaaaccga 
      ctgagaacc ggcgtaact gtgatctag ggtcaattt 
      tcccacatt cataactaa taaatttta actttggta 
      ctttacata cctgcactg atttgatgt tcaaattta 
      caattttca ccaaatctg caagtacct tgcaaaaaa 
      tacttgcaa acaaccatc gcaaaaaga ggaaaaaat 
      tgttagatc tgaaaaatt gttgaggtt acttactat 
      acaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY