Status
Please wait ...
Gene information
General information
TC034831 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7358744..7368404
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034831
    iBeetle screen iB_11042

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034831 RA
      MPTSPGLRA DMTSWGQHI DEKFHKFGV KCQGDGKLT 
      EKCTSLTPL SVIVSTIST PRMLNHQTP KKTLLVALL 
      LLAIIFLTK PQDSGLFNQ LYYFFHPKE SIQCYHTKS 
      QTLPDISDA RPAKGRSIF FHETSCNSF LNGKITITA 
      RQACAVESA ARLNPNFEI HLLFASPGI FKFEGTQSD 
      RFLQNLMTY PNVRIHHVD YERYTKGTP VETLYRKGK 
      IEVSGYAQS HASDVLRYI TLWKFGGIY LDLDVIVTK 
      PLESLPLNY AGAESDRNV AAGVLSFSP EGLGHELAQ 
      RCLQDLSEN FKGYDWGYN GPGVITRLL KKLCGAETA 
      KEMQSKDCE GFKVFPVDA FYPIPWWDW RLYFDENLT 
      EKVLNISKD SHVIHVWNK HSGGTRVAA RGNSAYAVL 
      AQKFCPGIV NECGYFF
    CDS sequence
    • >TC034831 RA
      atgcccacg tctccgggt ctcagagca gatatgaca 
      tcgtgggga cagcatatc gatgaaaaa tttcataag 
      tttggtgtc aagtgtcaa ggcgatggt aaactgacc 
      gagaaatgc acatctctc acaccctta tctgtgata 
      gtctccacc atatccact cctagaatg ctcaaccac 
      caaaccccg aaaaagacc ctccttgtc gcactcctc 
      ctcttagca ataattttc ctgaccaag ccccaagac 
      tccggtctt ttcaaccag ctttactac tttttccac 
      cccaaagag agcatccaa tgctaccac accaaatct 
      cagactctt cccgacata tcggacgcg cgtcccgcc 
      aaaggaaga tccattttc ttccacgaa acctcctgt 
      aattcgttc ctcaacggc aaaatcacg ataacggcc 
      cggcaggcg tgtgccgtc gagtccgca gctagactc 
      aacccgaat ttcgaaatt cacctcctg ttcgcatcc 
      cccgggatc ttcaaattc gaggggacg cagtccgac 
      cgatttttg caaaatctg atgacctat cccaacgtg 
      cggatacac cacgttgac tacgaaagg tacacgaag 
      ggcacgccg gtcgagacg ctctacaga aagggcaaa 
      atcgaggtc tcgggctac gcacagtcg cacgccagc 
      gatgtcctc agatacata actttgtgg aaattcggc 
      ggaatttac ctcgacttg gacgtcatc gtcacaaag 
      cccctcgaa tcactcccc ctgaactac gcaggggcg 
      gaatcagac cgaaacgtg gccgccggc gtcctgagc 
      ttcagcccc gagggcctg ggccacgaa ctggcccag 
      cggtgcctc caggacctc agtgagaat ttcaagggg 
      tacgattgg ggctacaac gggcctgga gtcatcacc 
      cgcctcctc aaaaagctc tgcggggcg gaaacggcc 
      aaagaaatg cagagcaaa gactgtgaa gggttcaag 
      gtgtttccc gtcgacgcc ttctacccc atcccttgg 
      tgggactgg aggctctat ttcgatgaa aatctgacc 
      gaaaaagtg ctcaatatt tcgaaagat agccacgtt 
      attcatgtc tggaataag cacagtggc ggcacgagg 
      gtggcggca agggggaat tcggcgtat gccgttttg 
      gcgcagaaa ttttgcccc gggattgtt aacgaatgc 
      gggtatttt ttttag
    mRNA sequence
    • >TC034831 RA
      gatcaataa aatgcccac gtctccggg tctcagagc 
      agatatgac atcgtgggg acagcatat cgatgaaaa 
      atttcataa gtttggtgt caagtgtca aggcgatgg 
      taaactgac cgagaaatg cacatctct cacaccctt 
      atctgtgat agtctccac catatccac tcctagaat 
      gctcaacca ccaaacccc gaaaaagac cctccttgt 
      cgcactcct cctcttagc aataatttt cctgaccaa 
      gccccaaga ctccggtct tttcaacca gctttacta 
      ctttttcca ccccaaaga gagcatcca atgctacca 
      caccaaatc tcagactct tcccgacat atcggacgc 
      gcgtcccgc caaaggaag atccatttt cttccacga 
      aacctcctg taattcgtt cctcaacgg caaaatcac 
      gataacggc ccggcaggc gtgtgccgt cgagtccgc 
      agctagact caacccgaa tttcgaaat tcacctcct 
      gttcgcatc ccccgggat cttcaaatt cgaggggac 
      gcagtccga ccgattttt gcaaaatct gatgaccta 
      tcccaacgt gcggataca ccacgttga ctacgaaag 
      gtacacgaa gggcacgcc ggtcgagac gctctacag 
      aaagggcaa aatcgaggt ctcgggcta cgcacagtc 
      gcacgccag cgatgtcct cagatacat aactttgtg 
      gaaattcgg cggaattta cctcgactt ggacgtcat 
      cgtcacaaa gcccctcga atcactccc cctgaacta 
      cgcaggggc ggaatcaga ccgaaacgt ggccgccgg 
      cgtcctgag cttcagccc cgagggcct gggccacga 
      actggccca gcggtgcct ccaggacct cagtgagaa 
      tttcaaggg gtacgattg gggctacaa cgggcctgg 
      agtcatcac ccgcctcct caaaaagct ctgcggggc 
      ggaaacggc caaagaaat gcagagcaa agactgtga 
      agggttcaa ggtgtttcc cgtcgacgc cttctaccc 
      catcccttg gtgggactg gaggctcta tttcgatga 
      aaatctgac cgaaaaagt gctcaatat ttcgaaaga 
      tagccacgt tattcatgt ctggaataa gcacagtgg 
      cggcacgag ggtggcggc aagggggaa ttcggcgta 
      tgccgtttt ggcgcagaa attttgccc cgggattgt 
      taacgaatg cgggtattt tttttagtg ctcaaaacg 
      tatcatggt gacatatcg ctccacatt atcattgtt 
      ctagacgta ggagagtac aataattac ctgaggcag 
      tatttacgc attgtgatt gttgtttat tgtttaacc 
      gtttggcaa taaattttt gatcatgga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY