Status
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Gene information
General information
TC012389 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 6045497..6052894
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012389
    iBeetle screen iB_11040

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 2

    mature egg not present - Number of animals showing the phenotype: 2

    egg chamber mostlynot present - Number of animals showing the phenotype: 2

    previtellogenic egg chamber not present - Number of animals showing the phenotype: 2

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    Comment: Only few eggs are present (10-12 eggs)
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012389 RA
      MSYMQQNQN RPLPQGHFQ RASADLPMM SAKEQTQMW 
      HQNSYMGDS GIHSGASTQ VPSITGKDE EMDLFDLDT 
      HRFTQNHDE IAQPGAQTV PATIFPEAL DEGIEISSP 
      QFQQCVPEP PNDLEKQNA VNLMDYQDE ADLVTRTIP 
      QLTKLLTDE DQVVVSHAA MMVNKLSNK ESFRHAIMS 
      SPEMLLALV RAISNSNDL ETTKSAIGA VHKLSQHRQ 
      GSLAILKCG GVPVLVQLL SSQVESVLF FAITTLHNL 
      LLHQEEAKV AVRHAGGLQ KLVSLLRRN NVNFLTIVT 
      DCLQILAYG NQESKLIIL ASQGSTELV RIMRTYDYE 
      RLLWTTSRV LKVLSVCSS NKPVIIESG GLLALGKHL 
      RSPSLRLVR NCLWTLRNL SDAATKVSG LDELLVSLV 
      ESLNSKDIQ VVTCAAGIL SNLTCNNEW NKKVVYEMG 
      GIDALIRTV LIAENREEI TEPLICTLR HLTSKSGFS 
      DRARRDIIE NNGVQVIIK LLNTPCSWP LVKALIGLL 
      RNLALYPSN VGPLRECGA VHHLIQLLM RGFQDIQKR 
      GSGPQKSSN GVRMEDIVE GTVNTLHIL SRDPFTRSI 
      IRQQMVIPI FIQLLYNEV ENVQRAAAA VLSELVVDK 
      EGADAIEQE GAATVLNEL LQSRNEGVA SYAAAILFR 
      LNEEKSQNS SKRSPNAMN SLFQEDSLW TPDFEIAEL 
      QEILSSDQT YSSVYNQET SGVHNNHGS QPFQQQGFN 
      QVPVEMSGL EIGTHDSGN SSSYSALLE AMEVTMADT 
      ESDLNFNNI NQLPSTPQD NQATEWYDN NILKTEF
    CDS sequence
    • >TC012389 RA
      atgagctac atgcaacag aaccagaac agacccctg 
      ccgcaaggg cacttccaa cgtgcttca gccgacctg 
      cccatgatg tccgccaaa gagcagacc caaatgtgg 
      caccagaac tcctacatg ggggactct ggcatccac 
      tccggggct tccacccag gtcccctcg atcacgggc 
      aaggacgaa gagatggac ttgttcgac ttggacacc 
      caccgattc acgcaaaac cacgacgag atcgcccaa 
      ccgggggcc cagaccgtc cctgcgacc atcttcccc 
      gaagccctc gacgagggg atcgaaatc tcctccccg 
      cagttccag caatgcgtc ccggagccc cccaacgac 
      ctggagaag caaaacgcc gtcaacttg atggactac 
      caggacgag gccgatctg gtcactcgc acaatcccc 
      cagttgacc aaactgcta actgacgag gaccaagtg 
      gtggtctca cacgccgct atgatggtc aataagctg 
      tcgaacaag gagtcgttc cggcatgcc attatgagc 
      agccccgaa atgctgctc gctctcgtc cgtgccatt 
      tccaattcg aatgatttg gagaccacc aaaagtgcc 
      atcggggct gttcataag ttgtcgcag cacaggcaa 
      ggctcactg gcgatactc aagtgtggg ggtgtgcct 
      gttttggtg cagcttctg agctcgcaa gtcgaaagt 
      gtgctgttt tttgccatc actactttg cataatctg 
      ctgctgcat caggaggag gccaaggtg gcggtgagg 
      catgctggg gggctccag aagttggtg tcgcttttg 
      aggaggaat aatgtgaat ttcttgacg attgttacg 
      gattgtttg caaattttg gcgtatggg aatcaggag 
      agtaagctg attattttg gcgtcgcaa gggtccact 
      gagcttgtg aggattatg aggacgtat gattatgag 
      aggttgctg tggacaacc tctagagtc ttaaaggtg 
      ttatcggtt tgctctagc aacaagccg gtgataatc 
      gaatctggc ggtttgcta gccctcggc aaacacctc 
      cgcagtccc agtcttcgt ctcgtacga aactgtttg 
      tggactctt cgtaattta tcggacgcc gcaacaaaa 
      gtgagcggt ttagacgaa ctactcgta tctttggtt 
      gaatcactc aattcgaaa gatatccaa gtagtaaca 
      tgtgccgcg ggaatcctt tccaatcta acgtgcaat 
      aacgaatgg aacaaaaaa gtcgtctac gaaatgggc 
      ggcattgac gctcttatt cgcaccgtt ctcatcgcc 
      gaaaatcgt gaagaaatc accgaacct ctgatctgc 
      actttaaga caccttact tcaaaaagc ggcttctct 
      gaccgcgcc agaagagac attattgaa aataacggc 
      gtccaagtg attatcaaa ttgttaaac acgccatgt 
      agttggccc ttggttaaa gccttgatc gggcttttg 
      cgtaacttg gccctttat cccagcaat gtgggccct 
      ttgagagaa tgtggggcc gttcatcac ttgatccaa 
      ctgttgatg cgcggcttt caagacatt cagaagagg 
      ggaagtggg cctcaaaag tcctcaaat ggggtccga 
      atggaggac atcgtcgaa ggtacagtc aacacgttg 
      cacatttta tcacgtgac cctttcaca cggtccata 
      attcgtcaa caaatggtt atcccgatt tttatccaa 
      ttgttgtac aacgaagtg gagaatgtg caacgtgcc 
      gccgctgcg gtgctcagc gagcttgtg gtcgataag 
      gaaggggcc gatgctatc gagcaggag ggggcggcc 
      acggttttg aacgagttg cttcaatcg cgcaatgaa 
      ggggttgcc tcgtatgca gcggcaatt ttgttcaga 
      ctgaacgaa gagaagtcg cagaatagc tcgaagcgg 
      tcgccgaac gcgatgaat tcgctgttc caagaggac 
      agtttgtgg acgcccgac ttcgagatt gccgaattg 
      caagaaatt ttgagttct gatcaaacc tacagtagt 
      gtgtataat caagagaca agcggtgtg cacaacaat 
      cacggtagt cagcctttt cagcagcaa ggatttaac 
      caggtacca gtagagatg tccggactg gagattggc 
      acacacgat agcggtaat agttctagt tattcagcg 
      ttgttagaa gccatggaa gtcacaatg gctgataca 
      gaatccgat ttaaacttt aataatatc aatcaattg 
      ccttccaca ccacaagat aatcaagcg acagaatgg 
      tacgataat aatatttta aaaaccgag ttttaa
    mRNA sequence
    • >TC012389 RA
      cgcagccaa aacacaaat aataacttt atttttaaa 
      ccccataac caataaaaa tttggatcg aacagttgg 
      gcttagtaa ttcaattag ttcgatttc cctaatgct 
      cagttttgt ggaagcctt acttaagtt atcttccgt 
      gtaaacaag cttggattt gtatcgtgt aagcgccat 
      tttataacc ttgtttgtt atgatagtg gtgtggaaa 
      catggacgc gtttttaaa ctaattaaa ccagtgttt 
      attaattaa acaagtgga gttttttgt tggattagt 
      gtcttactg tgcgctgca atcgccatg agctacatg 
      caacagaac cagaacaga cccctgccg caagggcac 
      ttccaacgt gcttcagcc gacctgccc atgatgtcc 
      gccaaagag cagacccaa atgtggcac cagaactcc 
      tacatgggg gactctggc atccactcc ggggcttcc 
      acccaggtc ccctcgatc acgggcaag gacgaagag 
      atggacttg ttcgacttg gacacccac cgattcacg 
      caaaaccac gacgagatc gcccaaccg ggggcccag 
      accgtccct gcgaccatc ttccccgaa gccctcgac 
      gaggggatc gaaatctcc tccccgcag ttccagcaa 
      tgcgtcccg gagcccccc aacgacctg gagaagcaa 
      aacgccgtc aacttgatg gactaccag gacgaggcc 
      gatctggtc actcgcaca atcccccag ttgaccaaa 
      ctgctaact gacgaggac caagtggtg gtctcacac 
      gccgctatg atggtcaat aagctgtcg aacaaggag 
      tcgttccgg catgccatt atgagcagc cccgaaatg 
      ctgctcgct ctcgtccgt gccatttcc aattcgaat 
      gatttggag accaccaaa agtgccatc ggggctgtt 
      cataagttg tcgcagcac aggcaaggc tcactggcg 
      atactcaag tgtgggggt gtgcctgtt ttggtgcag 
      cttctgagc tcgcaagtc gaaagtgtg ctgtttttt 
      gccatcact actttgcat aatctgctg ctgcatcag 
      gaggaggcc aaggtggcg gtgaggcat gctgggggg 
      ctccagaag ttggtgtcg cttttgagg aggaataat 
      gtgaatttc ttgacgatt gttacggat tgtttgcaa 
      attttggcg tatgggaat caggagagt aagctgatt 
      attttggcg tcgcaaggg tccactgag cttgtgagg 
      attatgagg acgtatgat tatgagagg ttgctgtgg 
      acaacctct agagtctta aaggtgtta tcggtttgc 
      tctagcaac aagccggtg ataatcgaa tctggcggt 
      ttgctagcc ctcggcaaa cacctccgc agtcccagt 
      cttcgtctc gtacgaaac tgtttgtgg actcttcgt 
      aatttatcg gacgccgca acaaaagtg agcggttta 
      gacgaacta ctcgtatct ttggttgaa tcactcaat 
      tcgaaagat atccaagta gtaacatgt gccgcggga 
      atcctttcc aatctaacg tgcaataac gaatggaac 
      aaaaaagtc gtctacgaa atgggcggc attgacgct 
      cttattcgc accgttctc atcgccgaa aatcgtgaa 
      gaaatcacc gaacctctg atctgcact ttaagacac 
      cttacttca aaaagcggc ttctctgac cgcgccaga 
      agagacatt attgaaaat aacggcgtc caagtgatt 
      atcaaattg ttaaacacg ccatgtagt tggcccttg 
      gttaaagcc ttgatcggg cttttgcgt aacttggcc 
      ctttatccc agcaatgtg ggccctttg agagaatgt 
      ggggccgtt catcacttg atccaactg ttgatgcgc 
      ggctttcaa gacattcag aagagggga agtgggcct 
      caaaagtcc tcaaatggg gtccgaatg gaggacatc 
      gtcgaaggt acagtcaac acgttgcac attttatca 
      cgtgaccct ttcacacgg tccataatt cgtcaacaa 
      atggttatc ccgattttt atccaattg ttgtacaac 
      gaagtggag aatgtgcaa cgtgccgcc gctgcggtg 
      ctcagcgag cttgtggtc gataaggaa ggggccgat 
      gctatcgag caggagggg gcggccacg gttttgaac 
      gagttgctt caatcgcgc aatgaaggg gttgcctcg 
      tatgcagcg gcaattttg ttcagactg aacgaagag 
      aagtcgcag aatagctcg aagcggtcg ccgaacgcg 
      atgaattcg ctgttccaa gaggacagt ttgtggacg 
      cccgacttc gagattgcc gaattgcaa gaaattttg 
      agttctgat caaacctac agtagtgtg tataatcaa 
      gagacaagc ggtgtgcac aacaatcac ggtagtcag 
      ccttttcag cagcaagga tttaaccag gtaccagta 
      gagatgtcc ggactggag attggcaca cacgatagc 
      ggtaatagt tctagttat tcagcgttg ttagaagcc 
      atggaagtc acaatggct gatacagaa tccgattta 
      aactttaat aatatcaat caattgcct tccacacca 
      caagataat caagcgaca gaatggtac gataataat 
      attttaaaa accgagttt taatttgtg ataatcaat 
      tattgcatg ctttgtaga cattgcaaa acaggtggc 
      aatattttc attttttcg tatttgttt ttgttgtat 
      tcctattac tttaggtta aggaacgtt tagttgtat 
      cacgaccgt ataagccat ttatcttag attggtagc 
      aataacgtg tattgttag aaatactta actagagcg 
      tcattattt atttttaat acgtaacat aattttata 
      attatcgta tatttacct atgttaaaa gtcccaagc 
      actgccttt tatttagta tatgttcgt atctttaca 
      ataaatata tactccaga t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY