Status
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Gene information
General information
TC014551 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 11530821..11533177
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC014551
      iBeetle screen iB_10091

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      larval musculature random pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      larva partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      iBeetle screen iB_11036

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC014551 RA
        MVNFTFRGL MAPTLTAFK NDWTVDLSV IQDYAKFLA 
        DSKIMGVLV NGTSGEGMS LNVRERKQV AEEWVKAVK 
        KTNQHLMVQ VGGAPLPDV LELAKHAES IGVHSLLCL 
        PDLYNKPAN SADLIRYLK TVGAAAPNT PLLYYHIPS 
        YTNVNIHMG QFLNDSVGK IPTFHGIKF TSTALDEGV 
        AAVEANGGK YAVFLGADQ LMAGAFTLG FDSAIATSL 
        NMFPQLGVK ILDAVKQSK LNSARETQQ LLTKVCSVV 
        TKHGPWVPT MKVAMDLFT PIKVGKARE PLTNITPEQ 
        VKEMGATMK TINYC
      CDS sequence
      • >TC014551 RA
        atggttaat tttacattt aggggcctt atggcacca 
        actctcact gcttttaaa aatgactgg acagtggat 
        ttaagtgtt attcaagat tatgcaaaa tttcttgct 
        gattcaaaa attatgggt gttttagta aatgggaca 
        agcggcgag gggatgtct ttgaacgta cgagaaaga 
        aagcaagtg gctgaagaa tgggtcaaa gcagtgaaa 
        aaaacaaac cagcatttg atggtacaa gtgggcgga 
        gcacccttg cctgatgtt ctggaactt gcaaaacat 
        gccgaatca attggagtg cattccctc ctttgctta 
        cccgacctt tacaacaaa cccgcaaat tcagctgat 
        ttgatcagg tacttgaaa actgtgggg gctgccgct 
        ccgaacacc ccccttttg tattaccac ataccgagt 
        tataccaac gtcaatatc catatggga caatttttg 
        aacgattcc gtcggtaaa atcccgact ttccacgga 
        attaaattc acttcgacc gccttagac gagggtgtg 
        gctgccgtt gaagccaat gggggcaaa tacgcggtg 
        tttttaggg gctgatcag ttaatggca ggggctttc 
        actttgggt tttgactcc gcaattgca acaagtcta 
        aatatgttt ccccaattg ggagtgaaa atcctggac 
        gcggtcaaa caatcgaaa ctaaatagc gcaagagag 
        acgcaacaa cttttaact aaagtttgc tccgtcgtt 
        accaagcat gggccttgg gtgcccacc atgaaagtc 
        gccatggac ctatttaca cccattaaa gtgggcaaa 
        gcacgagaa ccattgacc aatatcacc ccagaacaa 
        gtgaaggaa atgggggcc acaatgaaa acaatcaat 
        tattgttaa
      mRNA sequence
      • >TC014551 RA
        tttattatg tgaaagaca agataataa aaacttaaa 
        acatctttt ctgtgttat atttgtgtt gtgcctccg 
        tgcttatct ctgttaaac atggttaat tttacattt 
        aggggcctt atggcacca actctcact gcttttaaa 
        aatgactgg acagtggat ttaagtgtt attcaagat 
        tatgcaaaa tttcttgct gattcaaaa attatgggt 
        gttttagta aatgggaca agcggcgag gggatgtct 
        ttgaacgta cgagaaaga aagcaagtg gctgaagaa 
        tgggtcaaa gcagtgaaa aaaacaaac cagcatttg 
        atggtacaa gtgggcgga gcacccttg cctgatgtt 
        ctggaactt gcaaaacat gccgaatca attggagtg 
        cattccctc ctttgctta cccgacctt tacaacaaa 
        cccgcaaat tcagctgat ttgatcagg tacttgaaa 
        actgtgggg gctgccgct ccgaacacc ccccttttg 
        tattaccac ataccgagt tataccaac gtcaatatc 
        catatggga caatttttg aacgattcc gtcggtaaa 
        atcccgact ttccacgga attaaattc acttcgacc 
        gccttagac gagggtgtg gctgccgtt gaagccaat 
        gggggcaaa tacgcggtg tttttaggg gctgatcag 
        ttaatggca ggggctttc actttgggt tttgactcc 
        gcaattgca acaagtcta aatatgttt ccccaattg 
        ggagtgaaa atcctggac gcggtcaaa caatcgaaa 
        ctaaatagc gcaagagag acgcaacaa cttttaact 
        aaagtttgc tccgtcgtt accaagcat gggccttgg 
        gtgcccacc atgaaagtc gccatggac ctatttaca 
        cccattaaa gtgggcaaa gcacgagaa ccattgacc 
        aatatcacc ccagaacaa gtgaaggaa atgggggcc 
        acaatgaaa acaatcaat tattgttaa ttgtaaagg 
        gggtctcaa taaaaacgc gtcaagagt gctttgaaa 
        attttaata acacttgtg tccatcatt cattgttac 
        agaaatcta ttatctaat cacttgaaa ttctcacag 
        cactcttga ccatgtttt tgtttagga ccccgatat 
        tttattaaa aagtgttac aaataaaat atataaata 
        tggataaaa agcagtctt tagtcttga tggggcgaa 
        taaaacttt gatggcaat actggtgac acgctattg 
        cttccaaat cgttcgtta aacgagcga ttctgggac 
        tggtagcgc atttcaatg actttgtca taaaaccgt 
        gacaattta acagcattt gaggaacca gctgcgaga 
        tatcgcgcc tgtcgcgtg tccaggatg atttcatcg 
        gccccaaac tggctggca aaagtacct gtcgtagat 
        ttcctctca tcaatctga tcaagcttc gtcccaatg 
        acaagaatg ggcattgga gtattacca aagaaattc 
        tctgggtcg tattcatcc atgctactg attgaagtt 
        agcgatgtt tgattaacg tcatggttt atcaattct 
        tgcaaccat ttgtggaga ttcaattcg gactttcgg 
        tttgttaaa tcatgaact agaatcacc ccgtggcaa 
        gggttgtaa aatacaggt cttgtgttt ctgtgatta 
        ttgcttccg cccacatcc cagaattca ataaagtag 
        gttttctgg tcctttgtc ccttccttg tactcgtgg 
        agcttcact tccactgaa cacccgacc gtccaactg 
        ggggacctt atgggctcg ttgtgtgcg acaaggtgg 
        acgaaggag gttttgcca acgccggag tcgcccaac 
        accataata cgcacccgg tctattgcc gtcattatt 
        ttttatcac acaattaca ctttacaac tgggaaata 
        tttgaggtt atctgcgtt gtcaatgtc aacttgaca 
        cacaactgg tgtgcgtga ccgtaaatt tccagtcgc 
        ttatttcaa ataatgaag gtaaatttg taaattggc 
        accgtcaag tcaatttac aaattaaaa acaatgcaa 
        ataattgac taagagtca c
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY