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Gene information
General information
TC012424 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7143279..7171283
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012424
    iBeetle screen iB_11035

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012424 RA
      MECGVTGGY TNGTTPAGI LADVRSERS 
      PSKTGLHVN FKDKGEIKE RREKFLTAK 
      YGHHQMSLI RKRLAVEMW LYEELKKLY 
      ETSKQVDDV NNDEVEVDI DELLDMDND 
      DERRRHLQN LLINAKGPQ SDVKKFIND 
      LLDKAKTL
    • >TC012424 RB
      MECGVTGGY TNGTTPAGI LADVRSERS 
      PSKTGLHVN FKDKGEIKE RREKFLTAK 
      YGHHQMSLI RKRLAVEMW LYEELKKLY 
      ETSKQVDDV NNDEVEVDI DELLDMDND 
      DERRRHLQV TFPFKHLVL
    CDS sequence
    • >TC012424 RA
      atggaatgc ggcgtgacg gggggctac accaacggc 
      accactccg gccggcatc ctggccgac gtccggtcc 
      gaaaggtcg ccctcgaag acgggcctc cacgtcaat 
      ttcaaggac aaaggcgag atcaaggag cgcagagag 
      aagtttctc actgccaaa tacggacac catcagatg 
      agtctcatc agaaaacga ctggctgtg gaaatgtgg 
      ctttacgaa gagcttaaa aagctctac gaaacttcg 
      aaacaagta gacgatgta aataacgac gaagtagaa 
      gtggacata gacgaactg ctagatatg gacaacgac 
      gacgagcga aggaggcat ttacagaat ctgctgata 
      aatgctaaa gggccgcag agtgacgta aaaaaattc 
      atcaacgat ctgttggac aaagcaaag actctgtaa
    • >TC012424 RB
      atggaatgc ggcgtgacg gggggctac accaacggc 
      accactccg gccggcatc ctggccgac gtccggtcc 
      gaaaggtcg ccctcgaag acgggcctc cacgtcaat 
      ttcaaggac aaaggcgag atcaaggag cgcagagag 
      aagtttctc actgccaaa tacggacac catcagatg 
      agtctcatc agaaaacga ctggctgtg gaaatgtgg 
      ctttacgaa gagcttaaa aagctctac gaaacttcg 
      aaacaagta gacgatgta aataacgac gaagtagaa 
      gtggacata gacgaactg ctagatatg gacaacgac 
      gacgagcga aggaggcat ttacaggta acgttccca 
      ttcaaacac ttggtccta taa
    mRNA sequence
    • >TC012424 RA
      gcagtcgtt cgtggggtt tgcgtggat ttttagtgt 
      tcgtacctt tattgaaca ttttatgtg ttagtgttg 
      gtgattttg acgagaagg gtgtgcaga tcgggcggt 
      gcctttgtc ctttcggga ttatcattc atccagtgt 
      gagaaaaaa aataaaccc ccaatcaaa atggaatgc 
      ggcgtgacg gggggctac accaacggc accactccg 
      gccggcatc ctggccgac gtccggtcc gaaaggtcg 
      ccctcgaag acgggcctc cacgtcaat ttcaaggac 
      aaaggcgag atcaaggag cgcagagag aagtttctc 
      actgccaaa tacggacac catcagatg agtctcatc 
      agaaaacga ctggctgtg gaaatgtgg ctttacgaa 
      gagcttaaa aagctctac gaaacttcg aaacaagta 
      gacgatgta aataacgac gaagtagaa gtggacata 
      gacgaactg ctagatatg gacaacgac gacgagcga 
      aggaggcat ttacagaat ctgctgata aatgctaaa 
      gggccgcag agtgacgta aaaaaattc atcaacgat 
      ctgttggac aaagcaaag actctgtaa ataaagtga 
      agtgccaag taggttcag tgaagaaga cttcggccc 
      tatggcgcc tgtttgcac aagttttag tgattatcc 
      aattattgc atcatcgat gttgatcgt tcgaaactg 
      tattccatc ctaatctat caggaaggt caacgtggc 
      ttaagacag tttggaccg attcttttg taatagacg 
      aaattagtt tgtcgttgt ggccagttt gccaattgt 
      taatcgaga aaaatctct aacgtcttt ttctaattt 
      tttatattt ttagcgcta attttattg taatggata 
      caatgaatg ttgttacta tttgtgcat taaacgcgt 
      gtagctagt cctggtata gatcatatc acagaagcc 
      gccttgggg taattatca tggcttgag tagctctta 
      tcattggtt ctcatatcg aatgtgctc aaaattttt 
      atgtaatgt gatcatgaa tgtgagctt ccccaaatt 
      atcatcata cctatcgtc atattggcc tcactttgt 
      ttcgttaga tcgtttagt ataatgtaa ttgtttggt 
      gtacttata agatattta tctgtaaac tgcaatcga 
      tcgttgtga tctcagccc tctcaaatc tgacatgtg 
      tatcatagg atgtatgta ggaagtttt agctgtaag 
      acattttat gaattaaat agttatgat ccac
    • >TC012424 RB
      gcagtcgtt cgtggggtt tgcgtggat ttttagtgt 
      tcgtacctt tattgaaca ttttatgtg ttagtgttg 
      gtgattttg acgagaagg gtgtgcaga tcgggcggt 
      gcctttgtc ctttcggga ttatcattc atccagtgt 
      gagaaaaaa aataaaccc ccaatcaaa atggaatgc 
      ggcgtgacg gggggctac accaacggc accactccg 
      gccggcatc ctggccgac gtccggtcc gaaaggtcg 
      ccctcgaag acgggcctc cacgtcaat ttcaaggac 
      aaaggcgag atcaaggag cgcagagag aagtttctc 
      actgccaaa tacggacac catcagatg agtctcatc 
      agaaaacga ctggctgtg gaaatgtgg ctttacgaa 
      gagcttaaa aagctctac gaaacttcg aaacaagta 
      gacgatgta aataacgac gaagtagaa gtggacata 
      gacgaactg ctagatatg gacaacgac gacgagcga 
      aggaggcat ttacaggta acgttccca ttcaaacac 
      ttggtccta taactacaa gtcatcgat aaggaaaat 
      gtcaaggct tgtggcaga cccttcgac caatcaatt 
      tttataaag tattcaaac gatcgttgc tattttaag 
      caaacaaac cgcgcattg ctttcgtat ttatttttc 
      cagcgttcc ctcggggtc gcactatta aatatcctg 
      cgagagagc acttggctc ctgctgggc gtcgacctc 
      tcacatcgt tattgcgcg ctcaggatc acttttatt 
      tcaattttt taggccgct tcgcccccg gaattctga 
      ctacaaatt gaacgggtt atctggcgg gaatttcct 
      gttttttat tatttacac attaaacaa acactataa 
      ggcgctatg tcgccgccg accgtttcc tttcttcgc 
      cacgaggat aaacagaca atatttggc gttttgtaa 
      ttaaccttg tgattcgta aattacaat gtttagatt 
      ttgtggcct tcggctcca aaatacaaa ctgtaattt 
      gtagagttt tgcgtcaat aaatcgatt tattgcaat 
      gatgaaacg acttttgtt ccagaatct gctgataaa 
      tgctaaagg gccgcagag tgacgtaaa agtgagtgt 
      gtttcacgt ttggcgtgt gataacagt ttgtttcag 
      aaattcatc aacgatctg ttggacaaa gcaaagact 
      ctgtaaata aagtgaagt gccaagtag gttcagtga 
      agaagactt cggccctat ggcgcctgt ttgcacaag 
      ttttagtga ttatccaat tattgcatc atcgatgtt 
      gatcgttcg aaactgtat tccatccta atctatcag 
      gaaggtcaa cgtggctta agacagttt ggaccgatt 
      cttttgtaa tagacgaaa ttagtttgt cgttgtggc 
      cagtttgcc aattgttaa tcgagaaaa atctctaac 
      gtctttttc taatttttt atattttta gcgctaatt 
      ttattgtaa tggatacaa tgaatgttg ttactattt 
      gtgcattaa acgcgtgta gctagtcct ggtatagat 
      catatcaca gaagccgcc ttggggtaa ttatcatgg 
      cttgagtag ctcttatca ttggttctc atatcgaat 
      gtgctcaaa atttttatg taatgtgat catgaatgt 
      gagcttccc caaattatc atcatacct atcgtcata 
      ttggcctca ctttgtttc gttagatcg tttagtata 
      atgtaattg tttggtgta cttataaga tatttatct 
      gtaaactgc aatcgatcg ttgtgatct cagccctct 
      caaatctga catgtgtat cataggatg tatgtagga 
      agttttagc tgtaagaca ttttatgaa ttaaatagt 
      tatgatcca c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY