| General information | |||
| TC032630
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 26412785..26422443 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC032630 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_11033 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC032630 RA
MSLLQEFGN SCVHWSLGL TFLVGLIAS ETSGVVTVI ALPVSALSV TLSALCGAS LNPTDTYKK RKLCDILDF FQIWMEILE FLAASATCA RIASATIDY ISNEKLREW FLGIESHSL GEPWPDILG VSIVIVVIG LFMMGLEKS PFFTALLLV AVLVTFGFF ASVGCFETV VDLAKWSQD FKTCTMQTV ISGAAMCSY GFNSSYQKI TRHRLIKIV TMVFFPLFL YSFTTVVFT LMSHSKELA GTATPMLRV FELRDVDWA RLVMSICMI CTMCLALTE IFPSLYSLF EVLASKEWQ IFLASLRYR NSFTGAPLL AIFSAGSLS AILAFACPL AYLVKILNV STLLKAIAK SLLVLYYYY RPEFRNESY LVAPSSTQY SKLQSKNQK ETNLKNKIT GIISGWKKN VSPSRANRN RTPKEGTTQ DDEYLLLNE YCDKSDVVL NEVSSDEDE KESIVSSDV EDSYSSTDV DVAVQEYRD KIQVATIDN FNENKYPTY VTATTVVIF LIIITIATI SLSQVLIWK FYNLLWPCF TVSVFSTLI ILVMPQNLY QSENGLLPK SVTPLCNLI AIQLHIILV STILDEVWP GIVFWVSVG CLLFWRCDC CTCDGLFPN NNQKSKLNI TENFTDNYV DTILISR
- >TC032630 RA
atgagtctt ttgcaagaa ttcggaaac tcttgcgtc cactggtcg ctgggtttg accttcctt gtgggcctc atcgcctca gaaacctcc ggtgttgtc acagttatt gcgttacct gtttcagcc ctgtccgta acattatca gcattatgt ggcgcgtcc ttaaacccg actgacaca tacaagaaa cgaaaatta tgtgatatt ttagacttt ttccaaatc tggatggag attctagaa tttctagct gcatcagct acctgtgct aggattgct agcgctact atagactat atcagcaac gagaaatta agagagtgg tttctaggg attgaatcg cactcgttg ggcgagccc tggcctgat attttaggt gtttctatt gttattgtt gtcattgga ttatttatg atggggctt gagaaatcg ccctttttt accgccctt ctgttggtg gctgtgtta gtgactttt gggtttttt gcttcagtt ggctgcttc gaaaccgta gtggattta gcaaagtgg agccaggat tttaaaacg tgtacaatg caaacggtg atatcaggg gcagctatg tgttcatac ggcttcaac agttcttat cagaaaatc acgagacac agacttatc aaaatagtg acaatggtg ttttttccg ttattcttg tacagtttt accaccgta gttttcacg ttgatgtca cactccaag gaactggca ggaacagcg acccctatg ttgcgcgtt tttgaactt cgggacgtc gattgggcc cgtttagta atgtctatt tgtatgatt tgtacaatg tgcctcgcc ttgacggaa attttcccc tccttgtat tcactattt gaggttttg gcgagtaaa gagtggcaa attttttta gcgtctttg aggtacagg aactcgttt acgggggcc ccccttctg gcgattttt tcagcaggc agtctgagt gccattctg gcctttgcg tgtccactg gcctatctt gttaaaatt ttaaacgtc tcgactctc ctcaaagcc atcgcgaag tcattattg gtgctatat tactactac cggcccgaa tttcgcaac gagtcctat ttagttgct ccgagtagc acgcagtac agcaaactg caatcgaaa aatcaaaaa gaaacgaac ttgaaaaat aaaattacg ggtataatt tcgggttgg aagaaaaat gtctcgccg agtagagcc aacaggaat cgaactcca aaagaaggg acgacgcaa gacgatgag tatttgctc ctgaatgag tattgtgat aagtctgat gttgtttta aacgaagtc agttcggac gaagatgag aaagagtca attgtttcg agcgatgtt gaagattcg tacagttcg actgatgtt gatgttgca gttcaagaa tacagggac aaaatacaa gtcgcaacg atcgacaac ttcaacgag aacaaatac ccgacgtac gtgacagcg acgactgtt gtaatattt ttgataatt ataaccatc gcgacaata agtttatcg caagtgttg atttggaaa ttttacaat ttattatgg ccgtgtttt accgtttcg gtgttttcg actcttatt attttagtg atgcctcag aacttatac caatcagaa aacggtttg ttacctaaa tcggtcact ccattatgc aatctaatt gcgattcaa ctgcatatc attttggtg tccacgata ctggacgag gtctggccg ggaattgtt ttttgggtc agtgttggc tgtctgctt ttttggcga tgcgattgc tgcacctgt gatggactt tttcccaac aataaccaa aaatcaaaa ctgaacatc actgagaat ttcaccgac aattatgtg gacacgatt ttaatcagt agatga
- >TC032630 RA
ccccatcga tcgattaat gcagttgta atgcggaag atttatatt ttcgttaaa aatcagtaa aacaaacaa agttccatc aaaagccgt catgtcgac acaataata tcgcatcca caatacgta tttgaaacc gaatcggtt acgctaatg gattttctc tgaaaatga gtcttttgc aagaattcg gaaactctt gcgtccact ggtcgctgg gtttgacct tccttgtgg gcctcatcg cctcagaaa cctccggtg ttgtcacag ttattgcgt tacctgttt cagccctgt ccgtaacat tatcagcat tatgtggcg cgtccttaa acccgactg acacataca agaaacgaa aattatgtg atattttag actttttcc aaatctgga tggagattc tagaatttc tagctgcat cagctacct gtgctagga ttgctagcg ctactatag actatatca gcaacgaga aattaagag agtggtttc tagggattg aatcgcact cgttgggcg agccctggc ctgatattt taggtgttt ctattgtta ttgttgtca ttggattat ttatgatgg ggcttgaga aatcgccct tttttaccg cccttctgt tggtggctg tgttagtga cttttgggt tttttgctt cagttggct gcttcgaaa ccgtagtgg atttagcaa agtggagcc aggatttta aaacgtgta caatgcaaa cggtgatat caggggcag ctatgtgtt catacggct tcaacagtt cttatcaga aaatcacga gacacagac ttatcaaaa tagtgacaa tggtgtttt ttccgttat tcttgtaca gttttacca ccgtagttt tcacgttga tgtcacact ccaaggaac tggcaggaa cagcgaccc ctatgttgc gcgtttttg aacttcggg acgtcgatt gggcccgtt tagtaatgt ctatttgta tgatttgta caatgtgcc tcgccttga cggaaattt tcccctcct tgtattcac tatttgagg ttttggcga gtaaagagt ggcaaattt ttttagcgt ctttgaggt acaggaact cgtttacgg gggcccccc ttctggcga ttttttcag caggcagtc tgagtgcca ttctggcct ttgcgtgtc cactggcct atcttgtta aaattttaa acgtctcga ctctcctca aagccatcg cgaagtcat tattggtgc tatattact actaccggc ccgaatttc gcaacgagt cctatttag ttgctccga gtagcacgc agtacagca aactgcaat cgaaaaatc aaaaagaaa cgaacttga aaaataaaa ttacgggta taatttcgg gttggaaga aaaatgtct cgccgagta gagccaaca ggaatcgaa ctccaaaag aagggacga cgcaagacg atgagtatt tgctcctga atgagtatt gtgataagt ctgatgttg ttttaaacg aagtcagtt cggacgaag atgagaaag agtcaattg tttcgagcg atgttgaag attcgtaca gttcgactg atgttgatg ttgcagttc aagaataca gggacaaaa tacaagtcg caacgatcg acaacttca acgagaaca aatacccga cgtacgtga cagcgacga ctgttgtaa tatttttga taattataa ccatcgcga caataagtt tatcgcaag tgttgattt ggaaatttt acaatttat tatggccgt gttttaccg tttcggtgt tttcgactc ttattattt tagtgatgc ctcagaact tataccaat cagaaaacg gtttgttac ctaaatcgg tcactccat tatgcaatc taattgcga ttcaactgc atatcattt tggtgtcca cgatactgg acgaggtct ggccgggaa ttgtttttt gggtcagtg ttggctgtc tgctttttt ggcgatgcg attgctgca cctgtgatg gactttttc ccaacaata accaaaaat caaaactga acatcactg agaatttca ccgacaatt atgtggaca cgattttaa tcagtagat gacaagatt gtgacgaat acgaagacg caaatttta tttttgaat cagacgatg aaaacaatt aaatttgta tgcgatttt gtaccgtgt tacactatg tagaaattt tagagacat agggagagt attgattgc aataatgtg caataaaat atttgctta aaa
