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Gene information
General information
TC032630
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002500 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 26412785..26422443
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC032630
    iBeetle screen iB_11033

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032630 RA
      MSLLQEFGN SCVHWSLGL TFLVGLIAS ETSGVVTVI 
ALPVSALSV TLSALCGAS LNPTDTYKK RKLCDILDF 
FQIWMEILE FLAASATCA RIASATIDY ISNEKLREW 
FLGIESHSL GEPWPDILG VSIVIVVIG LFMMGLEKS 
PFFTALLLV AVLVTFGFF ASVGCFETV VDLAKWSQD 
FKTCTMQTV ISGAAMCSY GFNSSYQKI TRHRLIKIV 
TMVFFPLFL YSFTTVVFT LMSHSKELA GTATPMLRV 
FELRDVDWA RLVMSICMI CTMCLALTE IFPSLYSLF 
EVLASKEWQ IFLASLRYR NSFTGAPLL AIFSAGSLS 
AILAFACPL AYLVKILNV STLLKAIAK SLLVLYYYY 
RPEFRNESY LVAPSSTQY SKLQSKNQK ETNLKNKIT 
GIISGWKKN VSPSRANRN RTPKEGTTQ DDEYLLLNE 
YCDKSDVVL NEVSSDEDE KESIVSSDV EDSYSSTDV 
DVAVQEYRD KIQVATIDN FNENKYPTY VTATTVVIF 
LIIITIATI SLSQVLIWK FYNLLWPCF TVSVFSTLI 
ILVMPQNLY QSENGLLPK SVTPLCNLI AIQLHIILV 
STILDEVWP GIVFWVSVG CLLFWRCDC CTCDGLFPN 
NNQKSKLNI TENFTDNYV DTILISR
    CDS sequence
    • >TC032630 RA
      atgagtctt ttgcaagaa ttcggaaac tcttgcgtc 
cactggtcg ctgggtttg accttcctt gtgggcctc 
atcgcctca gaaacctcc ggtgttgtc acagttatt 
gcgttacct gtttcagcc ctgtccgta acattatca 
gcattatgt ggcgcgtcc ttaaacccg actgacaca 
tacaagaaa cgaaaatta tgtgatatt ttagacttt 
ttccaaatc tggatggag attctagaa tttctagct 
gcatcagct acctgtgct aggattgct agcgctact 
atagactat atcagcaac gagaaatta agagagtgg 
tttctaggg attgaatcg cactcgttg ggcgagccc 
tggcctgat attttaggt gtttctatt gttattgtt 
gtcattgga ttatttatg atggggctt gagaaatcg 
ccctttttt accgccctt ctgttggtg gctgtgtta 
gtgactttt gggtttttt gcttcagtt ggctgcttc 
gaaaccgta gtggattta gcaaagtgg agccaggat 
tttaaaacg tgtacaatg caaacggtg atatcaggg 
gcagctatg tgttcatac ggcttcaac agttcttat 
cagaaaatc acgagacac agacttatc aaaatagtg 
acaatggtg ttttttccg ttattcttg tacagtttt 
accaccgta gttttcacg ttgatgtca cactccaag 
gaactggca ggaacagcg acccctatg ttgcgcgtt 
tttgaactt cgggacgtc gattgggcc cgtttagta 
atgtctatt tgtatgatt tgtacaatg tgcctcgcc 
ttgacggaa attttcccc tccttgtat tcactattt 
gaggttttg gcgagtaaa gagtggcaa attttttta 
gcgtctttg aggtacagg aactcgttt acgggggcc 
ccccttctg gcgattttt tcagcaggc agtctgagt 
gccattctg gcctttgcg tgtccactg gcctatctt 
gttaaaatt ttaaacgtc tcgactctc ctcaaagcc 
atcgcgaag tcattattg gtgctatat tactactac 
cggcccgaa tttcgcaac gagtcctat ttagttgct 
ccgagtagc acgcagtac agcaaactg caatcgaaa 
aatcaaaaa gaaacgaac ttgaaaaat aaaattacg 
ggtataatt tcgggttgg aagaaaaat gtctcgccg 
agtagagcc aacaggaat cgaactcca aaagaaggg 
acgacgcaa gacgatgag tatttgctc ctgaatgag 
tattgtgat aagtctgat gttgtttta aacgaagtc 
agttcggac gaagatgag aaagagtca attgtttcg 
agcgatgtt gaagattcg tacagttcg actgatgtt 
gatgttgca gttcaagaa tacagggac aaaatacaa 
gtcgcaacg atcgacaac ttcaacgag aacaaatac 
ccgacgtac gtgacagcg acgactgtt gtaatattt 
ttgataatt ataaccatc gcgacaata agtttatcg 
caagtgttg atttggaaa ttttacaat ttattatgg 
ccgtgtttt accgtttcg gtgttttcg actcttatt 
attttagtg atgcctcag aacttatac caatcagaa 
aacggtttg ttacctaaa tcggtcact ccattatgc 
aatctaatt gcgattcaa ctgcatatc attttggtg 
tccacgata ctggacgag gtctggccg ggaattgtt 
ttttgggtc agtgttggc tgtctgctt ttttggcga 
tgcgattgc tgcacctgt gatggactt tttcccaac 
aataaccaa aaatcaaaa ctgaacatc actgagaat 
ttcaccgac aattatgtg gacacgatt ttaatcagt 
agatga
    mRNA sequence
    • >TC032630 RA
      ccccatcga tcgattaat gcagttgta atgcggaag 
atttatatt ttcgttaaa aatcagtaa aacaaacaa 
agttccatc aaaagccgt catgtcgac acaataata 
tcgcatcca caatacgta tttgaaacc gaatcggtt 
acgctaatg gattttctc tgaaaatga gtcttttgc 
aagaattcg gaaactctt gcgtccact ggtcgctgg 
gtttgacct tccttgtgg gcctcatcg cctcagaaa 
cctccggtg ttgtcacag ttattgcgt tacctgttt 
cagccctgt ccgtaacat tatcagcat tatgtggcg 
cgtccttaa acccgactg acacataca agaaacgaa 
aattatgtg atattttag actttttcc aaatctgga 
tggagattc tagaatttc tagctgcat cagctacct 
gtgctagga ttgctagcg ctactatag actatatca 
gcaacgaga aattaagag agtggtttc tagggattg 
aatcgcact cgttgggcg agccctggc ctgatattt 
taggtgttt ctattgtta ttgttgtca ttggattat 
ttatgatgg ggcttgaga aatcgccct tttttaccg 
cccttctgt tggtggctg tgttagtga cttttgggt 
tttttgctt cagttggct gcttcgaaa ccgtagtgg 
atttagcaa agtggagcc aggatttta aaacgtgta 
caatgcaaa cggtgatat caggggcag ctatgtgtt 
catacggct tcaacagtt cttatcaga aaatcacga 
gacacagac ttatcaaaa tagtgacaa tggtgtttt 
ttccgttat tcttgtaca gttttacca ccgtagttt 
tcacgttga tgtcacact ccaaggaac tggcaggaa 
cagcgaccc ctatgttgc gcgtttttg aacttcggg 
acgtcgatt gggcccgtt tagtaatgt ctatttgta 
tgatttgta caatgtgcc tcgccttga cggaaattt 
tcccctcct tgtattcac tatttgagg ttttggcga 
gtaaagagt ggcaaattt ttttagcgt ctttgaggt 
acaggaact cgtttacgg gggcccccc ttctggcga 
ttttttcag caggcagtc tgagtgcca ttctggcct 
ttgcgtgtc cactggcct atcttgtta aaattttaa 
acgtctcga ctctcctca aagccatcg cgaagtcat 
tattggtgc tatattact actaccggc ccgaatttc 
gcaacgagt cctatttag ttgctccga gtagcacgc 
agtacagca aactgcaat cgaaaaatc aaaaagaaa 
cgaacttga aaaataaaa ttacgggta taatttcgg 
gttggaaga aaaatgtct cgccgagta gagccaaca 
ggaatcgaa ctccaaaag aagggacga cgcaagacg 
atgagtatt tgctcctga atgagtatt gtgataagt 
ctgatgttg ttttaaacg aagtcagtt cggacgaag 
atgagaaag agtcaattg tttcgagcg atgttgaag 
attcgtaca gttcgactg atgttgatg ttgcagttc 
aagaataca gggacaaaa tacaagtcg caacgatcg 
acaacttca acgagaaca aatacccga cgtacgtga 
cagcgacga ctgttgtaa tatttttga taattataa 
ccatcgcga caataagtt tatcgcaag tgttgattt 
ggaaatttt acaatttat tatggccgt gttttaccg 
tttcggtgt tttcgactc ttattattt tagtgatgc 
ctcagaact tataccaat cagaaaacg gtttgttac 
ctaaatcgg tcactccat tatgcaatc taattgcga 
ttcaactgc atatcattt tggtgtcca cgatactgg 
acgaggtct ggccgggaa ttgtttttt gggtcagtg 
ttggctgtc tgctttttt ggcgatgcg attgctgca 
cctgtgatg gactttttc ccaacaata accaaaaat 
caaaactga acatcactg agaatttca ccgacaatt 
atgtggaca cgattttaa tcagtagat gacaagatt 
gtgacgaat acgaagacg caaatttta tttttgaat 
cagacgatg aaaacaatt aaatttgta tgcgatttt 
gtaccgtgt tacactatg tagaaattt tagagacat 
agggagagt attgattgc aataatgtg caataaaat 
atttgctta aaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY