Status
Please wait ...
Gene information
General information
TC012315 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 4098582..4100505
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012315
    iBeetle screen iB_11031

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012315 RA
      MENSHEKDG FLYIAINEV EPDFSDFAI KKLNELASV 
      IIINFGINR NIKPLLNPN HFKFVLTDP EGDLSYAND 
      IFKRVRVSR AKIVLILTR KPLPIFITY LLNSYAKIK 
      CFNVIVVYE SDGKIEVMT HNPFLPTFD KIVNLTNSP 
      LPFFYDKTR DLHGSTINC LFTFHDRTK IKKVRRNGK 
      VDFEGKDYN AIATIVAHL NGKLQIETM DNAFINPWF 
      EAINWPDRA SRKIKILEK RDISILIKS EQFTVDDQI 
      VENLYPHTQ NDLSILVPK ASEIPVGEL MAAIYKPST 
      WALNVTISL IVFLLVVTV RKLCKQRII IKDVFFEVW 
      SVLVINHVI HFPRHSLER LLIFGWILS CFVVNMFLQ 
      TKITSFLAI RLNHPDINT IEQLFESGL PLYSLPNYA 
      EEVQKKYNG TQYEKFTLA LLPLASNEG LMDQMTYKA 
      DVSQLPAFI IEHDIALFV SRCKNLRRN GAQMYHLVK 
      ESIIPNYQS YKVIHNSPL LPVLNKKLR RLGEGGFIE 
      LWAKRSIHN ATLEGFLSP EGVADADAV KPLTLDMTL 
      FLFYGLLLG LLFATMAFL AEVFVYEVT KPKQFIFLH
    CDS sequence
    • >TC012315 RA
      atggaaaac agccatgaa aaagacggc ttcctctac 
      atcgccatt aacgaagtc gagcccgac tttagcgac 
      tttgccata aaaaaactg aacgaactt gcaagcgtc 
      attattatt aatttcggc attaaccga aatattaag 
      ccattactc aatccaaat cattttaag ttcgtcctg 
      actgacccg gagggtgat ctctcgtac gccaatgat 
      attttcaaa agagtgaga gtttcacga gccaagatt 
      gtcctaatt ctcactcga aaacccctt ccgattttt 
      ataacgtat cttctaaac tcgtatgca aaaataaag 
      tgttttaac gtcatcgtt gtgtacgaa tcggacgga 
      aaaattgaa gtgatgacc cacaatcca tttttgcct 
      acattcgac aaaattgtt aacttgact aactcgcct 
      ttgcctttc ttctacgac aagacgaga gatctgcac 
      gggagcaca attaattgt ttgtttact tttcatgac 
      aggacgaaa ataaaaaaa gtgaggagg aacggaaaa 
      gtggatttt gagggaaag gattataac gcaattgcc 
      acaattgtg gcacactta aacgggaaa ctgcaaatt 
      gaaacgatg gacaatgcg tttataaat ccctggttt 
      gaggcaatc aattggcct gatagggca agtcgaaaa 
      atcaaaatt ttggagaag cgggacatt tcgattttg 
      atcaaatcg gagcagttt actgtcgat gaccaaatt 
      gtggagaat ttatatccc cacactcag aacgacttg 
      agtattttg gttccgaaa gcttcggaa ataccggtg 
      ggtgaatta atggcggca atttacaag ccctccact 
      tgggccctt aatgtgaca atttcttta attgttttt 
      ctcttagtt gtgactgtt agaaaatta tgcaaacaa 
      cgtattatc atcaaagac gtctttttc gaagtgtgg 
      tctgtcttg gtaataaac catgtaatc cactttcca 
      cggcacagt ttggaacgc ttgttaatt ttcgggtgg 
      atattgtcc tgttttgtt gtaaacatg ttcctgcaa 
      actaaaata accagcttt ctggccatc agactgaat 
      caccctgac ataaacacc atcgaacaa ttgtttgaa 
      tcaggactt ccactctac tcgctccca aactacgcc 
      gaagaagtc cagaagaaa tacaacgga acgcagtac 
      gaaaagttc actcttgca ttactacct ctggccagt 
      aatgaaggc ttgatggac caaatgact tacaaagcc 
      gatgtttca caactccct gcatttata atcgaacac 
      gatatcgca cttttcgtc tctagatgc aaaaatctg 
      aggagaaat ggggcgcaa atgtaccat ttggtcaaa 
      gagagtatc atcccgaat taccaatcg tacaaagtc 
      attcataat tctcctctt ttgcccgta ttgaataaa 
      aaattgagg aggttgggc gaaggtggt ttcattgaa 
      ctgtgggct aaaaggtcc attcataat gcgacactt 
      gaggggttt ttgtcaccg gaaggtgtt gcggacgct 
      gacgcagtc aaacctttg actctagac atgacttta 
      ttcttgttt tatgggcta cttttgggt cttttgttc 
      gcaacaatg gcgtttctg gctgaggtt tttgtttac 
      gaagtaacc aaacccaag cagtttata tttttacat 
      taa
    mRNA sequence
    • >TC012315 RA
      tcatggttc gcataatca tggaaaaca gccatgaaa 
      aagacggct tcctctaca tcgccatta acgaagtcg 
      agcccgact ttagcgact ttgccataa aaaaactga 
      acgaacttg caagcgtca ttattatta atttcggca 
      ttaaccgaa atattaagc cattactca atccaaatc 
      attttaagt tcgtcctga ctgacccgg agggtgatc 
      tctcgtacg ccaatgata ttttcaaaa gagtgagag 
      tttcacgag ccaagattg tcctaattc tcactcgaa 
      aaccccttc cgattttta taacgtatc ttctaaact 
      cgtatgcaa aaataaagt gttttaacg tcatcgttg 
      tgtacgaat cggacggaa aaattgaag tgatgaccc 
      acaatccat ttttgccta cattcgaca aaattgtta 
      acttgacta actcgcctt tgcctttct tctacgaca 
      agacgagag atctgcacg ggagcacaa ttaattgtt 
      tgtttactt ttcatgaca ggacgaaaa taaaaaaag 
      tgaggagga acggaaaag tggattttg agggaaagg 
      attataacg caattgcca caattgtgg cacacttaa 
      acgggaaac tgcaaattg aaacgatgg acaatgcgt 
      ttataaatc cctggtttg aggcaatca attggcctg 
      atagggcaa gtcgaaaaa tcaaaattt tggagaagc 
      gggacattt cgattttga tcaaatcgg agcagttta 
      ctgtcgatg accaaattg tggagaatt tatatcccc 
      acactcaga acgacttga gtattttgg ttccgaaag 
      cttcggaaa taccggtgg gtgaattaa tggcggcaa 
      tttacaagc cctccactt gggccctta atgtgacaa 
      tttctttaa ttgtttttc tcttagttg tgactgtta 
      gaaaattat gcaaacaac gtattatca tcaaagacg 
      tctttttcg aagtgtggt ctgtcttgg taataaacc 
      atgtaatcc actttccac ggcacagtt tggaacgct 
      tgttaattt tcgggtgga tattgtcct gttttgttg 
      taaacatgt tcctgcaaa ctaaaataa ccagctttc 
      tggccatca gactgaatc accctgaca taaacacca 
      tcgaacaat tgtttgaat caggacttc cactctact 
      cgctcccaa actacgccg aagaagtcc agaagaaat 
      acaacggaa cgcagtacg aaaagttca ctcttgcat 
      tactacctc tggccagta atgaaggct tgatggacc 
      aaatgactt acaaagccg atgtttcac aactccctg 
      catttataa tcgaacacg atatcgcac ttttcgtct 
      ctagatgca aaaatctga ggagaaatg gggcgcaaa 
      tgtaccatt tggtcaaag agagtatca tcccgaatt 
      accaatcgt acaaagtca ttcataatt ctcctcttt 
      tgcccgtat tgaataaaa aattgagga ggttgggcg 
      aaggtggtt tcattgaac tgtgggcta aaaggtcca 
      ttcataatg cgacacttg aggggtttt tgtcaccgg 
      aaggtgttg cggacgctg acgcagtca aacctttga 
      ctctagaca tgactttat tcttgtttt atgggctac 
      ttttgggtc ttttgttcg caacaatgg cgtttctgg 
      ctgaggttt ttgtttacg aagtaacca aacccaagc 
      agtttatat ttttacatt aattgtagg tacggtgtt 
      ttttacatt gccagttag acacatttt tttgaattt 
      ttattaata taaatattt aacgtaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY