Status
Please wait ...
Gene information
General information
TC015536 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2041503..2043844
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015536
    iBeetle screen iB_11030

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 2 - Number of animals showing the phenotype: 3
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015536 RA
      MPSSSSISV EGWDEESAY HEVLEYKDS 
      LKKEAEELL LKGFPATIV KLNALLETP 
      QFKNRKFTD VHQELNIPH IARRHQGHH 
      HHHHQHNHR PPVVILSGL GNSKEVPGL 
      VFGFDINEQ LLSEDNNKD FCCDDFIAR 
      YVAPETYTA TSHNHDKIV SFIGAAWEE 
      ALVNLSNGK VQYYNDLL
    CDS sequence
    • >TC015536 RA
      atgccctct tcgagctcc atttccgtg gagggctgg 
      gacgaggag tcggcctac cacgaggtg ttggaatac 
      aaagacagt ctgaagaaa gaggccgaa gagttgctg 
      cttaagggg ttccccgcg acgattgtg aagctcaac 
      gctttgcta gaaacgccc cagttcaaa aatcggaaa 
      ttcactgac gtgcaccag gagctgaac atcccgcat 
      atcgcaaga cggcaccaa ggtcaccac caccaccac 
      caccaacat aatcatcgt cctccagtg gttatttta 
      agtggttta ggcaacagt aaagaagtg ccaggattg 
      gtgttcggt ttcgatata aacgaacaa ctcctatcc 
      gaagacaac aataaagat ttctgctgc gatgatttc 
      attgcacgc tatgtggca ccggaaaca tacacggca 
      acgtcgcac aatcacgac aaaatcgtc agttttata 
      ggcgcagcc tgggaggaa gcactagtg aatttatcg 
      aacggtaaa gtgcagtac tataatgac ttactgtaa
    mRNA sequence
    • >TC015536 RA
      ctggtcaaa atttgaagt gatgccctc ttcgagctc 
      catttccgt ggagggctg ggacgagga gtcggccta 
      ccacgaggt gttggaata caaagacag tctgaagaa 
      agaggccga agagttgct gcttaaggg gttccccgc 
      gacgattgt gaagctcaa cgctttgct agaaacgcc 
      ccagttcaa aaatcggaa attcactga cgtgcacca 
      ggagctgaa catcccgca tatcgcaag acggcacca 
      aggtcacca ccaccacca ccaccaaca taatcatcg 
      tcctccagt ggttatttt aagtggttt aggcaacag 
      taaagaagt gccaggatt ggtgttcgg tttcgatat 
      aaacgaaca actcctatc cgaagacaa caataaaga 
      tttctgctg cgatgattt cattgcacg ctatgtggc 
      accggaaac atacacggc aacgtcgca caatcacga 
      caaaatcgt cagttttat aggcgcagc ctgggagga 
      agcactagt gaatttatc gaacggtaa agtgcagta 
      ctataatga cttactgta agtactaac tagtcaaat 
      tgtgtatag tgcaattgt gggaacaat gctcgaaga 
      gggtgacta tttattttg atttttaat ttatcaagg 
      ggtcgataa gatataaat aatgtaaaa tttgtaaca 
      tagctgtat tatgtacca aaattgtag gcttttttg 
      atttttgaa gacgctgtg catagttta gacaaaaga 
      cacgtcgta tcgctgtag cgtttgtat agattatgg 
      aagaaaaat cataaaaaa atgtttgat gtacattag 
      acatattac atatatcgg gcgtgttgc ggcatttaa 
      gtgacactg aaacgtgca atgcgcccc catcttaat 
      agatatcac gttaaagtt ggtctttaa cattgttta 
      tttattttt ctgatcaag tcgccacac ggttttact 
      ctttgaaaa tgacacgat ttcaaacag ttaaaaccc 
      gattaatgt gtaaaaaat agttgtatt aagaaaaag 
      tgcgctaag tcatttatg atttcgttt ttgaataac 
      tagagtcta gctagtgaa gcgctagat tttataccg 
      atgatataa taatcgaat cgacggctc tagatactg 
      tagaccggc tcgattgta tttttgttg tggtgagga 
      aaaattgtt aaattcatt tcaagattg tacttacag 
      ttgtctctc ttttgggta actttaagt ttgtatttg 
      attaaaatt tattgttac acttttaaa tatgtttac 
      cgtgaacag aatttaact atgttcatt gctttgata 
      aaaatatac gattattta ttca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY