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Gene information
General information
TC009876 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 12812772..12814515
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC009876
      iBeetle screen iB_11029

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC009876 RA
        MRLFVLLLV ICTKSYTSA DDVNILCSS TFCSDYTEK 
        NNCRELPDS CKIQNSTYN GLMLPSPDP CNCCQYCLV 
        NLKENDFCA EGEDGGLVP DSICGPGLT CDGGVCKKM 
        SLAQVKDNT PECAKAAAD YEKRKAEGN IGTMEMPPN 
        CDDSGFYKP YKCNLGQTC SCVDKDGKR IFGEIPYTS 
        NADSILKCA CSRKYQDAF NFFERHLHP NEHFRCTES 
        GDYDTIQCI GDWCMCTDA SDGAPTFPS RSSVNISDI 
        SSTTLKDCF QEGVHEEGK YYYPCAEKY LQVLAEINS 
        YKKEGFSEV FEVEFPSCS LDGKYAPVQ KNEDGGYCA 
        NPDGTPMGD YKGDATEMN CNCAKTLLL IPEEANEKP 
        ECCSNGNFN RIQCRRGKC YCVDFDGYQ DVKNTDATK 
        EIEVDADSI EQLFCYEMD MCVYKNQ
      CDS sequence
      • >TC009876 RA
        atgcgcctt tttgtatta ttactagtt atttgtaca 
        aaaagttac accagtgcc gacgacgtt aacatattg 
        tgttcgtca acattttgt agcgactat acggaaaaa 
        aataactgt cgcgaactt ccagacagc tgtaaaata 
        cagaattca acctataat ggactaatg ctaccatcc 
        cctgatcct tgcaattgc tgccaatac tgcttagta 
        aacctcaag gagaacgat ttttgtgca gagggtgaa 
        gatggtggt ctggtacct gactcaatt tgcggccca 
        ggactcaca tgtgacggt ggagtttgc aaaaaaatg 
        agtttggca caagtcaag gataataca ccggaatgt 
        gcaaaagca gcagctgat tacgaaaaa agaaaagca 
        gagggaaac ataggcacc atggaaatg cctccaaat 
        tgtgacgat agcggtttt tacaaaccg tacaaatgc 
        aatcttgga caaacgtgt tcctgtgtg gacaaggac 
        ggtaaacga atttttggc gaaattccg tacactagc 
        aatgctgat tcaatactc aaatgcgct tgttcgaga 
        aaataccag gacgctttt aatttcttt gagcgtcat 
        ttgcaccca aatgagcat tttaggtgc acggagagt 
        ggagattat gatactata cagtgtatt ggtgattgg 
        tgtatgtgt accgatgcc tctgacgga gcccccacg 
        ttcccatcc agatcttct gttaacatt tcagatatt 
        tctagtaca acattaaaa gattgtttc caagaaggg 
        gtacacgaa gaaggcaag tattactac ccttgtgca 
        gaaaagtat ttgcaagtt ttggctgaa ataaacagc 
        tacaagaaa gaaggcttc agtgaagtt tttgaagtc 
        gaattcccc agctgtagt ctcgatggc aaatacgca 
        ccggttcaa aagaatgag gatggaggt tattgtgca 
        aaccccgac ggaacaccg atgggtgat tacaaagga 
        gacgcaacc gaaatgaac tgcaattgt gcgaaaact 
        ttgcttcta atcccggaa gaggcaaat gaaaaaccg 
        gagtgttgc agcaatggt aatttcaac cgaatacaa 
        tgtagaaga gggaagtgt tattgtgtg gattttgac 
        ggttaccag gatgtgaaa aatacagac gcgaccaaa 
        gaaatagaa gtcgatgct gatagcatc gagcagctg 
        ttttgttat gaaatggat atgtgtgtc tacaaaaac 
        caataa
      mRNA sequence
      • >TC009876 RA
        cgcaataaa accacaaaa cattgttaa aaatgcgcc 
        tttttgtat tattactag ttatttgta caaaaagtt 
        acaccagtg ccgacgacg ttaacatat tgtgttcgt 
        caacatttt gtagcgact atacggaaa aaaataact 
        gtcgcgaac ttccagaca gctgtaaaa tacagaatt 
        caacctata atggactaa tgctaccat cccctgatc 
        cttgcaatt gctgccaat actgcttag taaacctca 
        aggagaacg atttttgtg cagagggtg aagatggtg 
        gtctggtac ctgactcaa tttgcggcc caggactca 
        catgtgacg gtggagttt gcaaaaaaa tgagtttgg 
        cacaagtca aggataata caccggaat gtgcaaaag 
        cagcagctg attacgaaa aaagaaaag cagagggaa 
        acataggca ccatggaaa tgcctccaa attgtgacg 
        atagcggtt tttacaaac cgtacaaat gcaatcttg 
        gacaaacgt gttcctgtg tggacaagg acggtaaac 
        gaatttttg gcgaaattc cgtacacta gcaatgctg 
        attcaatac tcaaatgcg cttgttcga gaaaatacc 
        aggacgctt ttaatttct ttgagcgtc atttgcacc 
        caaatgagc attttaggt gcacggaga gtggagatt 
        atgatacta tacagtgta ttggtgatt ggtgtatgt 
        gtaccgatg cctctgacg gagccccca cgttcccat 
        ccagatctt ctgttaaca tttcagata tttctagta 
        caacattaa aagattgtt tccaagaag gggtacacg 
        aagaaggca agtattact acccttgtg cagaaaagt 
        atttgcaag ttttggctg aaataaaca gctacaaga 
        aagaaggct tcagtgaag tttttgaag tcgaattcc 
        ccagctgta gtctcgatg gcaaatacg caccggttc 
        aaaagaatg aggatggag gttattgtg caaaccccg 
        acggaacac cgatgggtg attacaaag gagacgcaa 
        ccgaaatga actgcaatt gtgcgaaaa ctttgcttc 
        taatcccgg aagaggcaa atgaaaaac cggagtgtt 
        gcagcaatg gtaatttca accgaatac aatgtagaa 
        gagggaagt gttattgtg tggattttg acggttacc 
        aggatgtga aaaatacag acgcgacca aagaaatag 
        aagtcgatg ctgatagca tcgagcagc tgttttgtt 
        atgaaatgg atatgtgtg tctacaaaa accaataaa 
        atgtaccta attctgact gaaaaaata aaaaaataa 
        aaagcaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY