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Gene information
General information
TC007873 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2022349..2025826
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007873
    iBeetle screen iB_11028

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007873 RA
      MTVTYTAEV ATCRGLGCF LKLLRRWKG SIYKLVWLD 
      LIVFLLVYY SFNFMYRFL LDDQGKEIF ENVVRYCKE 
      YSNLIPLSF VLGFYVSVV MTRWWNQYT AIPFPDPLA 
      VFVSATVHG QDERGRVMR RTIMRYVCL CVTMMFAMI 
      SPRVKKRFP TLDHFIEAG LLHQSEKDI IADMNKKFP 
      NYSKHWLPI MWAASIVTR ARKEGRIRD DFAVKTMLD 
      ELNKFRGQC GLLLSYDMI SVPLVYTQV VTLAVYSYF 
      LTTLMGQQW VEGNEYKFD LYFPVFTTL QFFFYVGWL 
      KVAESLINP FGDDDDDFE INWMVDRNL QVSYLIVDE 
      MHHDHPELV RDQYWDEVF PQQLPYTAS STRESHPLP 
      STANIQVHP NVESDLIGP SSLKIDELS GDMMNSGAI 
      TFSAKPGRG NSQTSMGMG NSVPDSRPI SRAGSVTSM 
      LKRFLSKED KPKPEESQE LTQVNVKNP TTSKKFATE 
      VIEEVDEQT TITSQKSAK ESRPSVLGV FGPPPHSEP 
      ISVPNSLNT RTRTVYDPF PLSTSAPVT QDDYKDTTD 
      NVSIGSSSS EKDEFSELK KKRDEERLN RLKVNLLRS 
      ISTTPNEDG EECEVLLDK DSSKEPQSP SSPASII
    CDS sequence
    • >TC007873 RA
      atgactgtc acatacaca gccgaagtg gcaacttgt 
      cgaggattg ggttgcttt ctcaaatta ttaagaaga 
      tggaaaggc agtatttac aagctggtc tggctggac 
      ctgatcgtt ttcctattg gtctattac agcttcaat 
      ttcatgtac cgctttctg ttagacgat cagggcaaa 
      gagattttc gaaaacgtg gtccgctat tgcaaagaa 
      tacagcaat ctgatcccg ttgtctttt gtgttgggt 
      ttctatgta tctgtagtg atgacacgt tggtggaac 
      caatacacc gccatacca ttccctgat cctttggcc 
      gtttttgtc agtgctacc gtccacgga caggatgag 
      cgagggcgt gtgatgaga cgcacgatc atgcgttat 
      gtgtgcctg tgtgttacc atgatgttc gctatgata 
      tcaccacga gtcaaaaaa cggttcccg actttggac 
      cattttata gaagccgga ctgctgcac cagagcgag 
      aaggacatc atcgccgac atgaacaaa aaattcccc 
      aactattcc aaacactgg ttgccgata atgtgggcg 
      gccagtata gtgacaagg gcgcgcaaa gagggccgt 
      attcgggac gattttgcc gtcaaaacg atgctcgac 
      gagttgaac aaatttcga ggacagtgc ggactttta 
      ctaagttac gacatgata agtgtgcct ctagtctac 
      acccaagtc gtcactttg gccgtttac agttatttc 
      ctcacgact ttaatgggc caacagtgg gtcgaggga 
      aacgagtac aaattcgac ttatatttc cccgttttt 
      acaactttg caatttttt ttctacgtc gggtggctc 
      aaagtggcc gaatcgttg atcaacccc ttcggggac 
      gacgacgac gacttcgaa atcaactgg atggtggac 
      cgaaacttg caagtctcg tatttaata gtcgatgaa 
      atgcaccac gaccatcca gaactggtc cgtgatcag 
      tactgggac gaagttttc ccgcaacaa ctcccctac 
      acggcgtcc tcgactcgc gaaagccac ccgctgccc 
      tcgacggcc aatatccaa gtgcacccg aacgtcgag 
      tccgattta atcggtcct tcttccctg aaaatcgac 
      gagctttcg ggcgatatg atgaacagt ggagcgatc 
      acgttctcg gcaaagccg ggacgtggt aattcgcaa 
      acttcgatg ggtatgggc aattctgtg cctgactcg 
      aggcccatt tcacgcgct gggtcggtc actagcatg 
      ttgaagcgg tttttgagc aaggaagat aagcccaag 
      ccggaggaa agtcaggag ttgacgcaa gtcaatgtt 
      aaaaatccc acgacttcg aagaagttt gccacggaa 
      gttatcgaa gaggtggac gagcagacg acgataacg 
      tcgcagaaa tcagccaaa gaaagccga cctagtgtt 
      ctgggggtg ttcgggccg ccgccgcac tcggaaccc 
      atcagtgtt cccaactct ctcaacact cggacaagg 
      accgtttat gatcctttt ccgttgtcc acgtctgcc 
      cccgtgacg caagacgac tataaagac accaccgat 
      aatgtcagt attggcagt tcatcgtct gagaaagac 
      gaattcagt gaactgaag aagaaacga gatgaagag 
      agactgaac agattaaaa gttaatctg ttgaggtct 
      atcagtacg acgccgaac gaagacggc gaagagtgc 
      gaagttttg ctagataaa gacagttcg aaagaacca 
      caaagtcca agttcacct gcttcaata atttaa
    mRNA sequence
    • >TC007873 RA
      cgttttgaa ttttttcaa attgtcaaa tcaaaaaaa 
      ttgcgcatg cgtgaatcg cccctaacc tatcttttt 
      ttgtgacag gttgagttt tttgtgtga atttgaatc 
      tttttttag aggtgttag gaagcatgt cagagtgaa 
      tcatgtttt ttgtaaatt ttgacaact ccctatagc 
      aaacatcac atgtttgca tatatcatt tagtttgtt 
      gtaattatt atgttgttg gtcaggtga cagtcatta 
      ttacttatt tgtctgcat taaattgca ttcaaatcg 
      attgattcg agttgcaat catgactgt cacatacac 
      agccgaagt ggcaacttg tcgaggatt gggttgctt 
      tctcaaatt attaagaag atggaaagg cagtattta 
      caagctggt ctggctgga cctgatcgt tttcctatt 
      ggtctatta cagcttcaa tttcatgta ccgctttct 
      gttagacga tcagggcaa agagatttt cgaaaacgt 
      ggtccgcta ttgcaaaga atacagcaa tctgatccc 
      gttgtcttt tgtgttggg tttctatgt atctgtagt 
      gatgacacg ttggtggaa ccaatacac cgccatacc 
      attccctga tcctttggc cgtttttgt cagtgctac 
      cgtccacgg acaggatga gcgagggcg tgtgatgag 
      acgcacgat catgcgtta tgtgtgcct gtgtgttac 
      catgatgtt cgctatgat atcaccacg agtcaaaaa 
      acggttccc gactttgga ccattttat agaagccgg 
      actgctgca ccagagcga gaaggacat catcgccga 
      catgaacaa aaaattccc caactattc caaacactg 
      gttgccgat aatgtgggc ggccagtat agtgacaag 
      ggcgcgcaa agagggccg tattcggga cgattttgc 
      cgtcaaaac gatgctcga cgagttgaa caaatttcg 
      aggacagtg cggactttt actaagtta cgacatgat 
      aagtgtgcc tctagtcta cacccaagt cgtcacttt 
      ggccgttta cagttattt cctcacgac tttaatggg 
      ccaacagtg ggtcgaggg aaacgagta caaattcga 
      cttatattt ccccgtttt tacaacttt gcaattttt 
      tttctacgt cgggtggct caaagtggc cgaatcgtt 
      gatcaaccc cttcgggga cgacgacga cgacttcga 
      aatcaactg gatggtgga ccgaaactt gcaagtctc 
      gtatttaat agtcgatga aatgcacca cgaccatcc 
      agaactggt ccgtgatca gtactggga cgaagtttt 
      cccgcaaca actccccta cacggcgtc ctcgactcg 
      cgaaagcca cccgctgcc ctcgacggc caatatcca 
      agtgcaccc gaacgtcga gtccgattt aatcggtcc 
      ttcttccct gaaaatcga cgagctttc gggcgatat 
      gatgaacag tggagcgat cacgttctc ggcaaagcc 
      gggacgtgg taattcgca aacttcgat gggtatggg 
      caattctgt gcctgactc gaggcccat ttcacgcgc 
      tgggtcggt cactagcat gttgaagcg gtttttgag 
      caaggaaga taagcccaa gccggagga aagtcagga 
      gttgacgca agtcaatgt taaaaatcc cacgacttc 
      gaagaagtt tgccacgga agttatcga agaggtgga 
      cgagcagac gacgataac gtcgcagaa atcagccaa 
      agaaagccg acctagtgt tctgggggt gttcgggcc 
      gccgccgca ctcggaacc catcagtgt tcccaactc 
      tctcaacac tcggacaag gaccgttta tgatccttt 
      tccgttgtc cacgtctgc ccccgtgac gcaagacga 
      ctataaaga caccaccga taatgtcag tattggcag 
      ttcatcgtc tgagaaaga cgaattcag tgaactgaa 
      gaagaaacg agatgaaga gagactgaa cagattaaa 
      agttaatct gttgaggtc tatcagtac gacgccgaa 
      cgaagacgg cgaagagtg cgaagtttt gctagataa 
      agacagttc gaaagaacc acaaagtcc aagttcacc 
      tgcttcaat aatttaaat cagtattaa ttacggatc 
      tgcgcctaa gtaacgtta agcaaataa aatgttaga 
      taagtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY