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Gene information
General information
TC009701 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4641999..4645055
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009701
    iBeetle screen iB_11026

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4

    egg chamber not present - Number of animals showing the phenotype: 4

    vitellarium size decreased - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009701 RA
      MKRLLLLVC LSTLIICTT PKKEKCLTQ EDCENMTSE 
      YEKYRACME RRTKRSTDC YDEDSCDNC DCNTCSYNS 
      CDSCSHKDS CCGQCCQSN ACTTTNCCH QTCRAECRS 
      NSCRTSCKK RCLDRTTQT QSSSQMSHR HESTSKHNI 
      TTVIHLNNV INNTNLINL PINVNNTVL NNITNEGHS 
      GGGYFGSYV QGASKEQCC TTVGPRQCV PQNDFPFMR 
      CFHVRRQTC GSMCTAPVV HYQKHEICD NDVNLQQPC 
      RQQIVYIPQ PQPRCAYQP TWPYVTCGN SQSQSCEGC 
      YDHYIKRED LTKCSNFCY DDGYGAGPY YRQGPFYRP 
      GFAHVPSCV QTGTCGYGW DGYEGYGGA PSGFPYMYP 
      PQTYPYIIE DCENGTCAE PAIQWSGPV DEKSIDIKD 
      DKNIQWFQP DKMMNIETE VKNSPRSGR YAEIRIKHA 
      KKHKTKATT STTVESSSA GTSPTTAAS VVIATTTAS 
      N
    CDS sequence
    • >TC009701 RA
      atgaaacgc cttttgctc cttgtttgc ctctcaacc 
      ctcatcatc tgcaccacc cccaagaaa gaaaaatgc 
      ctaactcaa gaagattgc gaaaacatg acatccgaa 
      tacgaaaaa taccgcgct tgtatggaa cgccggacc 
      aagcgttcg accgattgc tacgacgaa gattcctgc 
      gacaactgc gactgcaac acttgcagt tacaattcc 
      tgcgactcc tgcagccac aaagacagc tgttgcggg 
      caatgctgc caaagcaac gcctgcaca acgaccaac 
      tgctgccac caaacctgc cgtgcagaa tgccgctcc 
      aattcctgc agaacctcc tgcaaaaaa cgctgtctg 
      gacagaaca acccaaacg caaagctcg tcccaaatg 
      tcgcacaga cacgaaagc accagcaaa cacaacata 
      accaccgtg attcatttg aacaacgtt atcaacaac 
      acgaattta attaacttg cccattaat gtaaataac 
      actgtcttg aacaacata acgaatgaa gggcatagt 
      ggagggggt tatttcggg agttacgtc caaggggcg 
      tcaaaggag caatgttgc acgacagtg ggacctcgc 
      caatgcgtc cctcagaac gattttccc ttcatgagg 
      tgtttccac gtccggaga caaacctgt ggcagcatg 
      tgcactgct ccagtcgtc cactaccaa aagcacgaa 
      atttgcgac aacgacgtc aatttgcaa caaccctgt 
      cggcagcaa atcgtctac atcccgcag ccgcaacct 
      aggtgtgcc taccagcca acctggccc tacgtcacg 
      tgcgggaac agccagtca cagtcgtgt gaaggctgt 
      tacgaccac tacatcaag cgcgaggat ttgaccaag 
      tgctcgaat ttctgctac gatgatggg tacggtgct 
      gggccctat tacaggcaa gggccgttc tacaggccg 
      gggttcgcc catgtgcct tcgtgcgtc cagaccggg 
      acctgcggc tatgggtgg gatgggtac gagggttat 
      gggggcgcg cccagcggg ttcccctac atgtacccg 
      cctcagacc tacccgtat attatcgag gattgtgag 
      aatgggacc tgtgccgag ccagccatt caatggagt 
      gggccagtg gatgagaag tcgattgat attaaggat 
      gataagaat attcagtgg tttcagccc gataaaatg 
      atgaatatt gagactgaa gtgaagaat tcgccaagg 
      tcggggagg tatgcggag ataaggatc aagcacgcc 
      aagaaacat aaaaccaaa gctacgact agcactacg 
      gtggaaagt tctagtgct ggtactagc cctacgact 
      gcggcgtct gttgtaata gcgactacg actgcgtca 
      aattga
    mRNA sequence
    • >TC009701 RA
      tattgcatt agtcgatgt ggtgaaggt ggtggttaa 
      cgacttgag ccgggaccg atccctcaa agatgaaac 
      gccttttgc tccttgttt gcctctcaa ccctcatca 
      tctgcacca cccccaaga aagaaaaat gcctaactc 
      aagaagatt gcgaaaaca tgacatccg aatacgaaa 
      aataccgcg cttgtatgg aacgccgga ccaagcgtt 
      cgaccgatt gctacgacg aagattcct gcgacaact 
      gcgactgca acacttgca gttacaatt cctgcgact 
      cctgcagcc acaaagaca gctgttgcg ggcaatgct 
      gccaaagca acgcctgca caacgacca actgctgcc 
      accaaacct gccgtgcag aatgccgct ccaattcct 
      gcagaacct cctgcaaaa aacgctgtc tggacagaa 
      caacccaaa cgcaaagct cgtcccaaa tgtcgcaca 
      gacacgaaa gcaccagca aacacaaca taaccaccg 
      tgattcatt tgaacaacg ttatcaaca acacgaatt 
      taattaact tgcccatta atgtaaata acactgtct 
      tgaacaaca taacgaatg aagggcata gtggagggg 
      gttatttcg ggagttacg tccaagggg cgtcaaagg 
      agcaatgtt gcacgacag tgggacctc gccaatgcg 
      tccctcaga acgattttc ccttcatga ggtgtttcc 
      acgtccgga gacaaacct gtggcagca tgtgcactg 
      ctccagtcg tccactacc aaaagcacg aaatttgcg 
      acaacgacg tcaatttgc aacaaccct gtcggcagc 
      aaatcgtct acatcccgc agccgcaac ctaggtgtg 
      cctaccagc caacctggc cctacgtca cgtgcggga 
      acagccagt cacagtcgt gtgaaggct gttacgacc 
      actacatca agcgcgagg atttgacca agtgctcga 
      atttctgct acgatgatg ggtacggtg ctgggccct 
      attacaggc aagggccgt tctacaggc cggggttcg 
      cccatgtgc cttcgtgcg tccagaccg ggacctgcg 
      gctatgggt gggatgggt acgagggtt atgggggcg 
      cgcccagcg ggttcccct acatgtacc cgcctcaga 
      cctacccgt atattatcg aggattgtg agaatggga 
      cctgtgccg agccagcca ttcaatgga gtgggccag 
      tggatgaga agtcgattg atattaagg atgataaga 
      atattcagt ggtttcagc ccgataaaa tgatgaata 
      ttgagactg aagtgaaga attcgccaa ggtcgggga 
      ggtatgcgg agataagga tcaagcacg ccaagaaac 
      ataaaacca aagctacga ctagcacta cggtggaaa 
      gttctagtg ctggtacta gccctacga ctgcggcgt 
      ctgttgtaa tagcgacta cgactgcgt caaattgag 
      atgattgta cttaatttt ttatttaga tttaattgt 
      gttccagta tttaatcga actaaaaga ccaaacttg 
      ttttctatt ctgttgtca tatgttttt atctaactt 
      gctgttctt tatttgcct tcatcacac atctattaa 
      aactttgtg gaaaaaacg aatttaaaa aaataaaaa 
      cttttttaa atcgatata tttccactc ttgtaattt 
      catagtttc aacactatt tcctaatta tcaatgtct 
      cagcttttc acaatttga gataaatga ttgtaccta 
      cttaattat tgccgcaga taagtttaa gttgtattt 
      cagtattgc tattgtatt taaacaatc aaaaggagc 
      agacgattt tttttattc tgctataat tatattttt 
      acttgatat ttcaaaata tatatcgtg atttatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY