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Gene information
General information
TC013541 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8260870..8264073
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013541
    iBeetle screen iB_11025

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013541 RA
      MKSNYFNVQ IIALFMLSL IPWPGPTVF KISCPRDNA 
      RIVRKIIQN KWIPILEKY KVSLPIECP FHPIRDIFG 
      PQQAAKQQH RPSQWTCGL CGKSFFEEK YLDMHFDNR 
      HKGFINMAE DAVCLADYC DIMRCDVLI TQDPKTVYP 
      DTVNTDIEI WREASSYTK ALATSGPRE VAKYTFKDG 
      IYPHLQKSG KATKPTKHC QKNEAPEAP NSNEERQSA 
      GDDDKLQNS SNNVCQNDL VDSALPAPD NRQQRIAEL 
      QKLKANCKP EELQKIKTK CEILVRDCI AGLLIQLSV 
      KDFKEVEDE LNRAVCWYL TCDRYWEDT QGDIRSFPW 
      GLLCMIIMV LSLGICMCY YIIWVLFDT DDVSVASAS 
      MTDRSTPSP AHVHRHEPP PQADGHQIY TEDYYLAPD 
      KDNEYIYVT YPPDLKRRL LESCYNRTT RL
    CDS sequence
    • >TC013541 RA
      atgaagtcc aattatttc aacgtgcag ataattgcg 
      ttgtttatg ctttcgttg atcccatgg ccggggccc 
      acagttttc aaaatctcg tgtccgcga gataatgcc 
      cgaatcgtg cggaaaata atccagaac aaatggata 
      ccgatttta gaaaaatac aaagtcagt ctgcccatc 
      gagtgcccc ttccacccc attagggac atcttcggg 
      ccccagcag gccgccaaa cagcagcac cggccctcg 
      caatggacg tgcggcctc tgcggcaag tcgtttttc 
      gaggagaaa tacctggac atgcatttc gacaatcgc 
      cataagggt ttcataaat atggccgaa gacgccgtg 
      tgccttgca gactactgc gacattatg agatgtgac 
      gtcttgata acgcaagac cccaaaacc gtctatcca 
      gacaccgtc aacacggat atagaaatc tggcgcgaa 
      gcttcctcg tacaccaaa gccttggcg acgtccggc 
      ccacgcgaa gtcgccaaa tacaccttc aaggatggg 
      atttacccc catctgcaa aaaagcgga aaagcaacg 
      aagcccacc aagcattgc cagaagaat gaagcaccg 
      gaagcgcct aatagcaac gaagagcgc caatctgca 
      ggcgacgac gataaattg cagaatagt tcgaataat 
      gtgtgtcaa aatgatttg gtggattcg gctttgccg 
      gcaccggac aacaggcag cagagaata gccgaactg 
      caaaagttg aaagcgaat tgtaaaccg gaagaactg 
      cagaaaatt aagacgaag tgcgagatt ttggtgcgc 
      gattgcatc gcggggctg ctaattcaa ctctcggtg 
      aaagatttt aaagaagtt gaagacgag ttaaacagg 
      gctgtctgt tggtacttg acgtgtgac cggtactgg 
      gaggatacg cagggggat atccgcagc ttcccttgg 
      ggtttgctc tgtatgata ataatggtt ctgtccctc 
      ggcatctgc atgtgttac tacatcatt tgggtcttg 
      ttcgacacc gatgacgtc agtgttgct agtgctagt 
      atgaccgat agatcgacg ccttcgccg gctcatgtc 
      catcggcat gagcctcca ccacaggca gacggtcac 
      caaatttac acagaggat tattacctg gctccagat 
      aaagataac gaatacata tacgtaact tatcctcct 
      gatttaaaa cgacggctt ttagaaagc tgctacaat 
      cgaactacc cgcttgtga
    mRNA sequence
    • >TC013541 RA
      aagtggcgt aataagtga ataattaac cgatttccg 
      aaggctaaa tgaccgtga atgtcgcct agcaacttg 
      cggcgacgt ccagcgtct tcggcgtcg tagcatacg 
      atgctttct aaatgccac aagaaaggt agagttttc 
      tttcatcct agtaccggt aaagaatat gaatgaagt 
      ccaattatt tcaacgtgc agataattg cgttgttta 
      tgctttcgt tgatcccat ggccggggc ccacagttt 
      tcaaaatct cgtgtccgc gagataatg cccgaatcg 
      tgcggaaaa taatccaga acaaatgga taccgattt 
      tagaaaaat acaaagtca gtctgccca tcgagtgcc 
      ccttccacc ccattaggg acatcttcg ggccccagc 
      aggccgcca aacagcagc accggccct cgcaatgga 
      cgtgcggcc tctgcggca agtcgtttt tcgaggaga 
      aatacctgg acatgcatt tcgacaatc gccataagg 
      gtttcataa atatggccg aagacgccg tgtgccttg 
      cagactact gcgacatta tgagatgtg acgtcttga 
      taacgcaag accccaaaa ccgtctatc cagacaccg 
      tcaacacgg atatagaaa tctggcgcg aagcttcct 
      cgtacacca aagccttgg cgacgtccg gcccacgcg 
      aagtcgcca aatacacct tcaaggatg ggatttacc 
      cccatctgc aaaaaagcg gaaaagcaa cgaagccca 
      ccaagcatt gccagaaga atgaagcac cggaagcgc 
      ctaatagca acgaagagc gccaatctg caggcgacg 
      acgataaat tgcagaata gttcgaata atgtgtgtc 
      aaaatgatt tggtggatt cggctttgc cggcaccgg 
      acaacaggc agcagagaa tagccgaac tgcaaaagt 
      tgaaagcga attgtaaac cggaagaac tgcagaaaa 
      ttaagacga agtgcgaga ttttggtgc gcgattgca 
      tcgcggggc tgctaattc aactctcgg tgaaagatt 
      ttaaagaag ttgaagacg agttaaaca gggctgtct 
      gttggtact tgacgtgtg accggtact gggaggata 
      cgcaggggg atatccgca gcttccctt ggggtttgc 
      tctgtatga taataatgg ttctgtccc tcggcatct 
      gcatgtgtt actacatca tttgggtct tgttcgaca 
      ccgatgacg tcagtgttg ctagtgcta gtatgaccg 
      atagatcga cgccttcgc cggctcatg tccatcggc 
      atgagcctc caccacagg cagacggtc accaaattt 
      acacagagg attattacc tggctccag ataaagata 
      acgaataca tatacgtaa cttatcctc ctgatttaa 
      aacgacggc ttttagaaa gctgctaca atcgaacta 
      cccgcttgt gagacctag tacgaagag atcgtttag 
      ctcaatttt tatttattt tgtagactt tttaaacag 
      actccatac tgaaaacat tccaaaatt ataagtagg 
      ggaatactc aaatgtatt aaagtttta caaaactta 
      atactttta agtaatttt ctgtttcta tctagattg 
      tagtttaga cataattcg aatttggta gccaatagc 
      tggactgag cctttttcc cttaaaaat actttaatt 
      ttaatcgaa cagtagatg gtggcataa tttttgaac 
      gtcatacat ttcgtacga aataaaatc ggaattttg 
      tttcactgc ttgctgtct catgccttg accattaac 
      atgaaaaaa acggaattt aaattcttg tccttgtac 
      ctaagataa tccaagctg aattccatc ctacgatta 
      ccaaacaaa ctcagatac aatttgtta caactaggt 
      gtagtcttt agcgtaata attagcttg atgttactt 
      tgtttttat tgcaacgat gtacataat gatagttgg 
      ataactttt gtacatgtg aaatacttt actatgcac 
      ctttttgag cattgcaat gaatgtaaa tttacatct 
      ttaaaacca tgttcataa tttataaaa atgtaataa 
      tattcaagg atcataata aagatttaa tattgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY