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Gene information
General information
TC007731 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 283739..284919
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007731
    iBeetle screen iB_11023

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages random not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head capsule not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax segment not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment potentially number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007731 RA
      MLNQVSVEA VYSATYVEN YLDCVENLP DELQRLISR 
      MRELDVCYL ARVREAAYQ TENWKKIGN DNPAKKARG 
      FARMQQALI AAQELGDEK MNLLQSILD KIEIKTRLL 
      DQDFKNLDF GKDETTQLE NKEQQPPNN NSSSAGLVE 
      RPPKRARRT RHDTFSGLE SNHNDSAPV EHVLRSQVP 
      STANSTKKS GTGKKKKRK SKQVQAQRE ESPPREEEP 
      IIDPDEPTY CLCDQISYG EMIMCDNDL CPIEWFHFS 
      CVTLTTKPK GKWYCPKCR GDRPNVMKP KAQFLRELE 
      KYNKDKEEK T
    CDS sequence
    • >TC007731 RA
      atgttgaac caagtgtcc gtcgaggcc gtttattcc 
      gccacgtac gtagagaac tatttggat tgcgttgag 
      aacctcccc gatgaactc caacgccta atctccagg 
      atgcgagaa ctcgacgtc tgttacttg gcccgcgtg 
      cgtgaagcc gcataccag accgagaac tggaaaaaa 
      atcggtaac gataacccc gccaaaaag gcccggggc 
      ttcgcccgc atgcagcaa gccctgatc gcagcccag 
      gaactgggc gacgagaaa atgaacctc ttgcagtca 
      attctggac aagattgag atcaagacg cgcttgttg 
      gaccaggac ttcaaaaat ttagatttt ggtaaagac 
      gagacgact caactcgaa aacaaggag caacagccc 
      cctaataac aacagcagt agtgcgggt ttggtcgag 
      aggcctcca aagagggcg cggcgcacc cgtcatgac 
      actttctca ggcttggag tcgaatcat aatgattca 
      gccccagtg gagcacgtt ttgaggagc caggtgccg 
      tccacggcc aactcgacg aagaagagt gggactggg 
      aagaagaag aagcgcaaa tcgaagcaa gtgcaagca 
      cagagagag gaatctccg ccaagggag gaagagccg 
      attattgat cccgatgag cccacttat tgtctctgt 
      gatcaaatt tcttacggt gaaatgatt atgtgcgac 
      aatgatttg tgtccaatc gagtggttc cacttttct 
      tgcgttact ttaaccacc aaaccgaaa ggtaaatgg 
      tattgtcca aagtgtaga ggtgataga cccaatgtt 
      atgaaaccc aaggcgcaa tttttaaga gaactcgaa 
      aagtataat aaggataaa gaggagaaa acataa
    mRNA sequence
    • >TC007731 RA
      aacagcgat tgatgtcaa aaatggtgg cttatgttt 
      gaaatatgt gaagctgaa gtaaacctt taacctttt 
      tgtaacttt ccccgttga aaatcatgt tgaaccaag 
      tgtccgtcg aggccgttt attccgcca cgtacgtag 
      agaactatt tggattgcg ttgagaacc tccccgatg 
      aactccaac gcctaatct ccaggatgc gagaactcg 
      acgtctgtt acttggccc gcgtgcgtg aagccgcat 
      accagaccg agaactgga aaaaaatcg gtaacgata 
      accccgcca aaaaggccc ggggcttcg cccgcatgc 
      agcaagccc tgatcgcag cccaggaac tgggcgacg 
      agaaaatga acctcttgc agtcaattc tggacaaga 
      ttgagatca agacgcgct tgttggacc aggacttca 
      aaaatttag attttggta aagacgaga cgactcaac 
      tcgaaaaca aggagcaac agcccccta ataacaaca 
      gcagtagtg cgggtttgg tcgagaggc ctccaaaga 
      gggcgcggc gcacccgtc atgacactt tctcaggct 
      tggagtcga atcataatg attcagccc cagtggagc 
      acgttttga ggagccagg tgccgtcca cggccaact 
      cgacgaaga agagtggga ctgggaaga agaagaagc 
      gcaaatcga agcaagtgc aagcacaga gagaggaat 
      ctccgccaa gggaggaag agccgatta ttgatcccg 
      atgagccca cttattgtc tctgtgatc aaatttctt 
      acggtgaaa tgattatgt gcgacaatg atttgtgtc 
      caatcgagt ggttccact tttcttgcg ttactttaa 
      ccaccaaac cgaaaggta aatggtatt gtccaaagt 
      gtagaggtg atagaccca atgttatga aacccaagg 
      cgcaatttt taagagaac tcgaaaagt ataataagg 
      ataaagagg agaaaacat aagtttatt ggtgtgtgc 
      attttacag ttcttacat agcaattaa atataattt 
      catagaatg ttttaaaat aataaatat gtctaacat 
      ct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY