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Gene information
General information
TC013661 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6635008..6636738
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013661
    iBeetle screen iB_11021

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013661 RA
      MKIIIFLVC AVCFVGGLP SLKNASICK STYCKVEDD 
      CRCSSTTNP INNVEDPAP QLIAITVSE SIVTTLYHN 
      YLEHLLFGR TNPDGYPIG ATFYVNHEY TDYELVQKL 
      YLRGYEIGV HSITKNSSQ EYWRHASFN DLIEEFGGQ 
      RQIISHFAN IPAEDIRGG RTPQLQFEG DLTINAYKQ 
      VGLRYDNSW PTSSNKLIL PYTLDYLST QECLVTINC 
      PKESHEHFW IAPITNIRG VNNVECNSL VTCLVQGTA 
      EEIADWLIN EVDRVTAQN RAPLVLRLD SYWFEFTDN 
      SLEGFTLFL NEMSKRSDV FFVSVQDII DWIKNPVSV 
      TKYVTPIHK RSAECTPVN CALRFLDGS ERYMNSCVR 
      CPRTYPWKG NPLGE
    CDS sequence
    • >TC013661 RA
      atgaaaata ataattttt ctcgtctgt gctgtttgc 
      tttgttggg ggacttccc tcccttaaa aatgcaagc 
      atttgtaaa agcacatac tgcaaagtt gaagacgat 
      tgtcgctgt tcttctaca actaaccca atcaacaac 
      gtcgaagat cctgctcca caactaatt gctataaca 
      gtgagtgaa tctattgtt acaacactc tatcataac 
      tatttagaa catctgctc tttgggcgc acaaatccc 
      gatggatac ccgatcggg gcaacgttt tacgtcaac 
      cacgagtac acagattat gaattagtg caaaaactg 
      tatcttcgt ggttacgaa attggtgtc cattcaata 
      acaaaaaac tcttctcaa gagtactgg cggcacgcg 
      tcttttaat gaccttatt gaggagttc ggtggccag 
      cggcaaatt atatcgcac tttgctaac attccagct 
      gaggatatt cgaggtggt agaacacca caattgcaa 
      tttgaagga gacttaaca ataaacgca tataaacaa 
      gttggactt agatatgat aattcctgg cctacaagt 
      tctaacaaa cttattctt ccctacacg ctagactac 
      ctgtctact caagaatgt ttagttacg attaattgc 
      ccaaaggag tcacacgag catttttgg atagctcct 
      atcaccaat attagaggt gtcaacaac gtcgaatgt 
      aactctctg gtaacctgt ttagttcaa ggaaccgct 
      gaagaaatt gccgattgg ttaattaac gaagttgac 
      cgagtaaca gcacaaaat cgggcaccc ttagttttg 
      cggctagac tcgtattgg tttgaattt accgataac 
      agtttggaa ggttttact ttattttta aacgagatg 
      tcaaaaaga agtgatgtc ttctttgta tcggtccaa 
      gatattatt gattggatc aagaaccca gtttccgtt 
      acaaaatac gttacccca atacacaaa agatctgca 
      gagtgcact cctgttaat tgtgctctc aggtttttg 
      gatgggagc gaaaggtac atgaattct tgtgttcgg 
      tgtccacga acgtatccc tggaaaggg aatccattg 
      ggagaataa
    mRNA sequence
    • >TC013661 RA
      atctccaaa aaataaagg atagcaatt ttgtaatat 
      catattaaa aaattttaa taaattgtg ttacgttac 
      cgatttatt gatcaaact ttttgtacc acgcaatgt 
      ttaataatc ttgtttgta tttgaaagt caacgactt 
      gacgaattt gaaatagtt ttaggaaaa acattaaaa 
      cctttatca aatagcttc ctgcgatta ttgttgtaa 
      ttttaagaa cacttaatt ctcctgtcc cttttgatt 
      gacgagctt tgtgttatc tgatttgag gcaaccatt 
      tatattaaa ctgcacggt aaaaaatta atagttact 
      ttccgaacc acgtatatt aaagctaag aaatcatga 
      aaataataa tttttctcg tctgtgctg tttgctttg 
      ttgggggac ttccctccc ttaaaaatg caagcattt 
      gtaaaagca catactgca aagttgaag acgattgtc 
      gctgttctt ctacaacta acccaatca acaacgtcg 
      aagatcctg ctccacaac taattgcta taacagtga 
      gtgaatcta ttgttacaa cactctatc ataactatt 
      tagaacatc tgctctttg ggcgcacaa atcccgatg 
      gatacccga tcggggcaa cgttttacg tcaaccacg 
      agtacacag attatgaat tagtgcaaa aactgtatc 
      ttcgtggtt acgaaattg gtgtccatt caataacaa 
      aaaactctt ctcaagagt actggcggc acgcgtctt 
      ttaatgacc ttattgagg agttcggtg gccagcggc 
      aaattatat cgcactttg ctaacattc cagctgagg 
      atattcgag gtggtagaa caccacaat tgcaatttg 
      aaggagact taacaataa acgcatata aacaagttg 
      gacttagat atgataatt cctggccta caagttcta 
      acaaactta ttcttccct acacgctag actacctgt 
      ctactcaag aatgtttag ttacgatta attgcccaa 
      aggagtcac acgagcatt tttggatag ctcctatca 
      ccaatatta gaggtgtca acaacgtcg aatgtaact 
      ctctggtaa cctgtttag ttcaaggaa ccgctgaag 
      aaattgccg attggttaa ttaacgaag ttgaccgag 
      taacagcac aaaatcggg cacccttag ttttgcggc 
      tagactcgt attggtttg aatttaccg ataacagtt 
      tggaaggtt ttactttat ttttaaacg agatgtcaa 
      aaagaagtg atgtcttct ttgtatcgg tccaagata 
      ttattgatt ggatcaaga acccagttt ccgttacaa 
      aatacgtta ccccaatac acaaaagat ctgcagagt 
      gcactcctg ttaattgtg ctctcaggt ttttggatg 
      ggagcgaaa ggtacatga attcttgtg ttcggtgtc 
      cacgaacgt atccctgga aagggaatc cattgggag 
      aataatacc gagccacac ccaaatttc gatatccta 
      ttgtttctt gtgtaattt ttggtgaaa tttaaatta 
      aagacatat tatctat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY