Status
Please wait ...
Gene information
General information
TC012378 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5860497..5863515
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012378
    iBeetle screen iB_11018

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012378 RA
      MGKRQHQKD KMYLTYTEW TTLYGGKKS GYTTAEEDY 
      FKRLPFDHC CLSLQPWEI PYCDLDGNI FDLEPLLPF 
      LKKFKINPV TGKPLDFKS LVQLNFHKN EEGEFECPV 
      LYKQFTKSS HIVAIATTG NVYLMEAVE QLNIKNKNW 
      KDLISDTPF ERKDIIMLQ DPLDLNKFN ISNFHHVKN 
      NLRIETEEE IKEKNDPQG HLKNVNPET KYTLEELKR 
      DYKEPTIEI NKVEVKEKA DKFNAAHYS TGAVAASFT 
      STAMVPRLV HEAAVKHED EVRYDRVKK KGYVRLITN 
      LGPLNLEIY CDEVPRASE NFIRHCASG YYNGTKFHR 
      SIRNFMVQG GDPTGTGTG GESIWGKKF VDEFRPNLQ 
      HVGRGVLSM ANSGKNTNG SQFFITYRS CKHLNNKHT 
      IFGRLVGGM ETLNEMEKI EVDNKDKPI EDIVLIRAQ 
      VFVNPFEEA DEQLAKERE EELKRQKEE EEAKRRKAE 
      AQKTLKVYK SGVGKYINP DNVKSSTLD ESDLTEPQQ 
      KKKKKMKFT FNDW
    CDS sequence
    • >TC012378 RA
      atggggaag cgccagcac cagaaagac aaaatgtac 
      ttgacgtac acagagtgg accactctt tacgggggc 
      aaaaaatcg ggttatacg accgcggag gaggactat 
      tttaagcgg ctcccgttt gaccactgc tgcctgtcg 
      ttgcaaccg tgggaaata ccctactgt gacttggac 
      ggcaatatt tttgatttg gagcctttg ctccccttt 
      ttgaagaag ttcaagata aacccagtc acgggaaag 
      cctctggat ttcaagtcg ttggtgcag ttgaatttc 
      cacaaaaat gaagaaggc gagtttgag tgccccgtc 
      ctttacaaa caattcacc aaaagctcc cacattgtg 
      gcgattgcc accactgga aacgtgtac ttgatggaa 
      gcagtggaa cagcttaat attaagaac aaaaactgg 
      aaggactta atcagtgat acgcccttc gaacgcaag 
      gatatcatc atgttacag gacccacta gacttaaat 
      aagttcaac atttccaac ttccaccac gtcaaaaac 
      aatttacga atcgaaacc gaggaggaa atcaaggag 
      aagaacgac cctcaaggc cacttgaaa aacgtgaac 
      cctgaaaca aaatacacc cttgaggag ctaaaacgg 
      gactacaaa gagccaaca attgaaata aacaaagtc 
      gaagttaag gaaaaggcg gacaagttc aacgctgcc 
      cattattca acgggggct gtggctgcg agtttcacc 
      tcaacagcg atggtcccc cgattggtg cacgaagcc 
      gcagtgaag cacgaagat gaggtgcga tacgacaga 
      gtcaagaaa aaaggctat gtgaggtta atcacgaac 
      ttggggccc ctcaacttg gagatttac tgcgacgaa 
      gtgccacgt gcttcggag aattttatc aggcattgt 
      gcgagtggc tattacaac gggacgaaa tttcacaga 
      tctattcgg aattttatg gttcaaggg ggcgacccg 
      acggggaca gggactggg ggcgaatcc atttggggg 
      aagaaattc gttgatgaa ttcaggccc aatttgcaa 
      catgtgggg cgaggggtc ttgtctatg gccaacagt 
      gggaaaaac acaaatggg tcacagttt ttcataaca 
      tacaggtcg tgcaagcac ttgaataat aagcacacg 
      atttttggt cgtttagtt ggtggcatg gagacttta 
      aacgaaatg gaaaaaatt gaagtggac aataaggat 
      aagcccatt gaggatatt gtgcttata agggcgcaa 
      gtttttgtg aatccattt gaagaggcg gacgaacaa 
      ttggcgaaa gaacgcgaa gaggaattg aaaagacag 
      aaggaggaa gaagaggcg aagagacgt aaagcggaa 
      gctcaaaaa accctaaaa gtgtataaa agtggtgtt 
      gggaaatat atcaatcct gataacgtg aaaagttca 
      acccttgat gagagtgac ttgacagaa ccgcaacag 
      aagaagaag aaaaaaatg aaatttacg tttaatgat 
      tggtaa
    mRNA sequence
    • >TC012378 RA
      gttgtgaca gcgagctaa cctcaaagt cgtgtgatt 
      tatattttt taattttac ctctttaca agttacagc 
      aaaatgggg aagcgccag caccagaaa gacaaaatg 
      tacttgacg tacacagag tggaccact ctttacggg 
      ggcaaaaaa tcgggttat acgaccgcg gaggaggac 
      tattttaag cggctcccg tttgaccac tgctgcctg 
      tcgttgcaa ccgtgggaa ataccctac tgtgacttg 
      gacggcaat atttttgat ttggagcct ttgctcccc 
      tttttgaag aagttcaag ataaaccca gtcacggga 
      aagcctctg gatttcaag tcgttggtg cagttgaat 
      ttccacaaa aatgaagaa ggcgagttt gagtgcccc 
      gtcctttac aaacaattc accaaaagc tcccacatt 
      gtggcgatt gccaccact ggaaacgtg tacttgatg 
      gaagcagtg gaacagctt aatattaag aacaaaaac 
      tggaaggac ttaatcagt gatacgccc ttcgaacgc 
      aaggatatc atcatgtta caggaccca ctagactta 
      aataagttc aacatttcc aacttccac cacgtcaaa 
      aacaattta cgaatcgaa accgaggag gaaatcaag 
      gagaagaac gaccctcaa ggccacttg aaaaacgtg 
      aaccctgaa acaaaatac acccttgag gagctaaaa 
      cgggactac aaagagcca acaattgaa ataaacaaa 
      gtcgaagtt aaggaaaag gcggacaag ttcaacgct 
      gcccattat tcaacgggg gctgtggct gcgagtttc 
      acctcaaca gcgatggtc ccccgattg gtgcacgaa 
      gccgcagtg aagcacgaa gatgaggtg cgatacgac 
      agagtcaag aaaaaaggc tatgtgagg ttaatcacg 
      aacttgggg cccctcaac ttggagatt tactgcgac 
      gaagtgcca cgtgcttcg gagaatttt atcaggcat 
      tgtgcgagt ggctattac aacgggacg aaatttcac 
      agatctatt cggaatttt atggttcaa gggggcgac 
      ccgacgggg acagggact gggggcgaa tccatttgg 
      gggaagaaa ttcgttgat gaattcagg cccaatttg 
      caacatgtg gggcgaggg gtcttgtct atggccaac 
      agtgggaaa aacacaaat gggtcacag tttttcata 
      acatacagg tcgtgcaag cacttgaat aataagcac 
      acgattttt ggtcgttta gttggtggc atggagact 
      ttaaacgaa atggaaaaa attgaagtg gacaataag 
      gataagccc attgaggat attgtgctt ataagggcg 
      caagttttt gtgaatcca tttgaagag gcggacgaa 
      caattggcg aaagaacgc gaagaggaa ttgaaaaga 
      cagaaggag gaagaagag gcgaagaga cgtaaagcg 
      gaagctcaa aaaacccta aaagtgtat aaaagtggt 
      gttgggaaa tatatcaat cctgataac gtgaaaagt 
      tcaaccctt gatgagagt gacttgaca gaaccgcaa 
      cagaagaag aagaaaaaa atgaaattt acgtttaat 
      gattggtaa tttgtgtaa ataaataaa taaaatttt 
      aagtatttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY