Status
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Gene information
General information
TC011689 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15044381..15046093
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011689
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_11017

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011689 RA
      MGSQLYLKL PSGQKLPAL GLGTWEAIS DEEIETAVN 
      NALDLDYRH IDTAWVYEN EAAIGKVLK KRFASGLKR 
      QDVFITTKL PMTGVHPDR VDMFIKKSL TNLQLDYVD 
      LYLIHFPVG CTYYEGEAR PRFNDKGEI ETEGKTDHV 
      ALWKKMEEQ VDAGRAKNI GLSNYNISQ IETVLKNAK 
      IKPAALQVE LHVYLQQRE LVEFCQKNG IAVIAYSPL 
      GNPGMNKFL QKMGQQPRQ LANILEDAK ISEIAKKHK 
      KTPAQVALR FLIQRGIAV IPKSVTPKR IDENRKLFD 
      FVLSDDEMK TLYGLNVGE AARVCDFKV FGGLDKHPD 
      FPFAQK
    CDS sequence
    • >TC011689 RA
      atgggctcg caactgtat ttgaagctc ccaagtggg 
      caaaaactg ccggctttg ggcctaggg acttgggag 
      gcgataagc gatgaagaa atcgaaact gcggtcaac 
      aacgcccta gatttagac tatcggcac attgacaca 
      gcttgggtt tatgaaaat gaggcggcg attgggaaa 
      gtgttgaaa aaacgcttt gcttccggc ttgaaaaga 
      caggacgtt tttattacg actaaactt ccgatgacg 
      ggggttcac ccagacagg gttgacatg tttataaaa 
      aagtcattg acgaatttg caattggat tacgtcgat 
      ttgtacttg attcatttc cccgttggt tgtacttat 
      tacgagggg gaagcacgg cccagattt aacgataaa 
      ggggagatt gagactgag ggaaagacc gaccacgtc 
      gccctgtgg aagaaaatg gaagagcag gttgatgcc 
      ggaagggcc aaaaatatc ggtttatca aactacaac 
      atttctcaa attgaaacc gtgctaaaa aacgcaaaa 
      attaaaccc gccgcactt caagtcgaa ctccacgtc 
      tatttgcaa caacgcgaa cttgtagaa ttttgtcaa 
      aaaaacgga attgctgtt attgcctac tctcctttg 
      gggaatccc ggcatgaac aagtttttg caaaaaatg 
      ggccaacaa cccagacag ttggcaaat attttggaa 
      gatgcaaaa attagtgag attgcaaaa aaacacaaa 
      aaaacacca gctcaagtg gcgttacgt ttcttgatt 
      cagaggggc attgcggtt attcctaaa agtgtaacg 
      ccgaaacga atcgatgaa aatcgaaaa ttgttcgat 
      tttgtgttg agtgacgat gagatgaaa actttgtat 
      ggtttaaat gtgggggag gcggccaga gtttgcgat 
      tttaaggtc tttggagga cttgataaa caccccgat 
      ttcccgttc gcacaaaaa tga
    mRNA sequence
    • >TC011689 RA
      tctggtcga ctgtactta taactggtt gcataacct 
      ccaccttat gtaataaca taaacacgg aagagccaa 
      taaaacctt tcagctgtt attttttca aggtttttc 
      taatttatt ctcatgttg aacaaactc acgagtggt 
      ctaagactg cttcgcatt tttaacaaa attcttgac 
      aatacttgt cagcaattc cattctgcg ttattttat 
      acaaaaatg ggctcgcaa ctgtatttg aagctccca 
      agtgggcaa aaactgccg gctttgggc ctagggact 
      tgggaggcg ataagcgat gaagaaatc gaaactgcg 
      gtcaacaac gccctagat ttagactat cggcacatt 
      gacacagct tgggtttat gaaaatgag gcggcgatt 
      gggaaagtg ttgaaaaaa cgctttgct tccggcttg 
      aaaagacag gacgttttt attacgact aaacttccg 
      atgacgggg gttcaccca gacagggtt gacatgttt 
      ataaaaaag tcattgacg aatttgcaa ttggattac 
      gtcgatttg tacttgatt catttcccc gttggttgt 
      acttattac gagggggaa gcacggccc agatttaac 
      gataaaggg gagattgag actgaggga aagaccgac 
      cacgtcgcc ctgtggaag aaaatggaa gagcaggtt 
      gatgccgga agggccaaa aatatcggt ttatcaaac 
      tacaacatt tctcaaatt gaaaccgtg ctaaaaaac 
      gcaaaaatt aaacccgcc gcacttcaa gtcgaactc 
      cacgtctat ttgcaacaa cgcgaactt gtagaattt 
      tgtcaaaaa aacggaatt gctgttatt gcctactct 
      cctttgggg aatcccggc atgaacaag tttttgcaa 
      aaaatgggc caacaaccc agacagttg gcaaatatt 
      ttggaagat gcaaaaatt agtgagatt gcaaaaaaa 
      cacaaaaaa acaccagct caagtggcg ttacgtttc 
      ttgattcag aggggcatt gcggttatt cctaaaagt 
      gtaacgccg aaacgaatc gatgaaaat cgaaaattg 
      ttcgatttt gtgttgagt gacgatgag atgaaaact 
      ttgtatggt ttaaatgtg ggggaggcg gccagagtt 
      tgcgatttt aaggtcttt ggaggactt gataaacac 
      cccgatttc ccgttcgca caaaaatga gctttttta 
      attgaaaca ttacaatgt gaccggatg ttggttgca 
      taacccatg atataaaat gattatgtt gtattttac 
      cctttactt aataaataa tcaaataaa ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY