Status
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Gene information
General information
TC002611 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 21538632..21545289
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC002611
      iBeetle screen iB_11016

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC002611 RA
        MSEIFKVGQ KFHSYSAVE KSLKDYEEQ TFAKFWKRD 
        ARLINSSRT LRRIREPLK YEEVKFCCI YGGQKFRPS 
        GSGQKEIRT FKKEELCPA HVSLRVTTD GKDLQVRSV 
        SDNHNHERS MVQFQYLCQ QRRLGRKAK ETVEELPAM 
        QANKMLVKQ KLQQETGKM LTLKDLSKN GGQKSEKNL 
        DECVDVLRT QYNAEVNIL KDEDNNFKG MLIQTSNMK 
        NTLKAYPEF LGIDGTYKL VNIDIPVFL IVSEDGNCQ 
        TEIVSFCLL TTADKDSFT WFLETFKAN NPQTRFTKC 
        IMADKDLLE KTVIKDKFP DANVNICIF HSMQIFSRA 
        ITCEKMSIT HAERVKSLK ILQKMVYSE TEEEFEQLH 
        YLLKISAPK PVTEYFDKN WYPVKDEWS LSKSFIKGC 
        FQNTTNNHI EKINAKLKS VINKNSSLV QFMHSFFTI 
        MSSLESEHD FKAISQLQK RLVTSYSPE SDEQKYLDF 
        LTNEAGKIV IKQIELSKK IQVPSVENN GINSTEGRQ 
        TCDPTNCTC NFKTSMLLP CRHIFAIRK RLELPLFLK 
        ELVHKRWTL DYYKICHRR LAQCPVFDT DIETSEAPN 
        VVVANKNNT KIYEQKRKE VLSVMESLV NLVAISSND 
        SFDQKMDNL KTLEMLWKQ GSRTGIVII KEEDEGELP 
        LENLNEQVI IKEEDEADE GELPLVNLN EIDEGKRTR 
        PVMIKEENE ADESEFHLV NSNELVMIK EEDDADEDE 
        LPLNLNEIA PDESNEDTI IQEPTISEV ASIKISPKI 
        KERGRPRGS ELTTAGLPK RQKLHVPFP KLTLEPKQK 
        KILGWFVSD EVVALAINE RLIIEEKYV ETRPENIPH 
        AVLHENVDM NIVRNFFSD TAWSVVENV IALKRQNKL 
        LCN
      CDS sequence
      • >TC002611 RA
        atgtcggag atattcaaa gttggccaa aagttccac 
        agctattct gctgtggaa aagtccctg aaagattac 
        gaggagcaa acttttgcg aaattctgg aaacgtgat 
        gcacgtctg attaattcc tcaagaacc ttacgtcga 
        attagagaa cctctaaaa tatgaagaa gtaaaattc 
        tgttgcatt tatggaggt cagaagttc agaccatcc 
        gggagtgga caaaaagaa attagaact ttcaagaaa 
        gaagagtta tgtcctgcc cacgtgtct ttgcgagtt 
        acaaccgac gggaaagat ctgcaagtg agaagtgtc 
        tcagacaac cataatcac gagagaagc atggttcag 
        tttcaatat ctctgtcaa caaaggaga ttgggcaga 
        aaagctaaa gagactgta gaagaatta ccagctatg 
        caagctaac aaaatgttg gtaaagcaa aaattacaa 
        caagaaacg ggaaaaatg cttacgtta aaagatctt 
        tccaaaaat ggtggacaa aaatctgaa aaaaactta 
        gatgaatgt gtagatgtt cttaggaca caatataat 
        gcagaagta aacatactt aaagatgag gataacaat 
        tttaaaggc atgctgatt caaacatct aatatgaaa 
        aatactctc aaggcatac ccggaattt ttgggaatt 
        gatggtacc tataaatta gtaaacatc gatatacct 
        gtgttccta atagtgtca gaagatggc aactgtcag 
        actgaaata gtttctttc tgtctattg accactgca 
        gacaaagac agttttact tggtttttg gaaaccttt 
        aaagcaaat aatccacag actagattc acaaagtgc 
        atcatggct gataaagac ttactagaa aaaacagtt 
        ataaaagac aagttccca gacgctaat gtgaacatt 
        tgtatcttt cactcaatg caaatattc agccgagca 
        attacctgt gaaaaaatg agtatcact cacgccgaa 
        agagttaag tcgctgaaa attttacaa aaaatggtt 
        tattccgag acagaagaa gagtttgaa cagttgcat 
        taccttcta aaaatatca gctccaaaa ccggttact 
        gaatatttt gacaaaaac tggtatcca gttaaggat 
        gaatggtca ttgtctaaa agtttcata aaaggttgt 
        tttcaaaac accacaaat aatcatatc gaaaaaata 
        aatgcgaaa ctcaagtcc gttatcaat aaaaacagc 
        tcattagta caatttatg cattccttt ttcaccatt 
        atgtcatct cttgagagt gaacatgat tttaaagcc 
        atatcccag ttacaaaaa agactcgtc acctcgtac 
        tcaccagaa agcgacgaa caaaaatat ctggatttt 
        ttaaccaac gaagctggt aaaatagtg ataaaacaa 
        attgagttg agcaaaaaa attcaagtt ccttcagtt 
        gaaaacaat ggtatcaac agtacggaa ggacgacaa 
        acatgtgat ccaactaat tgcacttgt aattttaaa 
        actagtatg ttgcttcct tgcagacac atttttgca 
        attcgaaag cgtctggaa ttgcctttg tttttgaaa 
        gagcttgtg cataagcgt tggacacta gattattat 
        aaaatttgt cacaggcgt ttggcacag tgcccagtg 
        tttgataca gatattgaa acatcggaa gcgcctaat 
        gtagttgta gcaaataaa aacaacacg aaaatatat 
        gaacagaaa cgaaaagaa gtgttaagt gtaatggag 
        tcgttggtg aatttggtc gccatatcc agcaatgat 
        tcatttgat caaaaaatg gataattta aaaactctt 
        gaaatgttg tggaagcaa ggcagtagg acaggaata 
        gtaataatc aaagaggag gatgaaggc gagttacca 
        ttagagaat ttgaacgaa caagtaata atcaaagag 
        gaggatgaa gcagatgaa ggcgaatta ccactagtc 
        aatttgaac gaaattgac gaaggcaaa aggacaaga 
        ccagtaatg atcaaagag gagaatgaa gcagatgaa 
        agcgaattt catttggtg aattcaaac gaattagta 
        atgattaaa gaggaagat gacgctgat gaagacgag 
        ttaccattg aatttgaac gaaattgcc cctgacgag 
        tcaaatgaa gacaccatt attcaagaa cccaccatc 
        agtgaggta gcaagtatt aaaatatct ccaaagatt 
        aaagaaaga ggacgtcct agaggtagc gaattaaca 
        actgctggt ctgccaaaa cggcaaaag ctacatgtg 
        ccctttccc aaacttaca ttggaacct aaacagaaa 
        aaaatattg ggttggttt gtttctgac gaagttgtg 
        gccttggct attaacgaa agactgatc attgaagag 
        aagtatgtg gaaacaaga ccggaaaat attcctcac 
        gcggtttta catgaaaat gtagatatg aatatagtt 
        cgtaacttt ttttctgat actgcgtgg agtgttgta 
        gaaaatgta atcgccctg aaaagacag aacaaattg 
        ctgtgtaat tag
      mRNA sequence
      • >TC002611 RA
        tgaaaattg tgttctgaa attgtgcct tcccctata 
        ataggttag gtcaagatg tcggagata ttcaaagtt 
        ggccaaaag ttccacagc tattctgct gtggaaaag 
        tccctgaaa gattacgag gagcaaact tttgcgaaa 
        ttctggaaa cgtgatgca cgtctgatt aattcctca 
        agaacctta cgtcgaatt agagaacct ctaaaatat 
        gaagaagta aaattctgt tgcatttat ggaggtcag 
        aagttcaga ccatccggg agtggacaa aaagaaatt 
        agaactttc aagaaagaa gagttatgt cctgcccac 
        gtgtctttg cgagttaca accgacggg aaagatctg 
        caagtgaga agtgtctca gacaaccat aatcacgag 
        agaagcatg gttcagttt caatatctc tgtcaacaa 
        aggagattg ggcagaaaa gctaaagag actgtagaa 
        gaattacca gctatgcaa gctaacaaa atgttggta 
        aagcaaaaa ttacaacaa gaaacggga aaaatgctt 
        acgttaaaa gatctttcc aaaaatggt ggacaaaaa 
        tctgaaaaa aacttagat gaatgtgta gatgttctt 
        aggacacaa tataatgca gaagtaaac atacttaaa 
        gatgaggat aacaatttt aaaggcatg ctgattcaa 
        acatctaat atgaaaaat actctcaag gcatacccg 
        gaatttttg ggaattgat ggtacctat aaattagta 
        aacatcgat atacctgtg ttcctaata gtgtcagaa 
        gatggcaac tgtcagact gaaatagtt tctttctgt 
        ctattgacc actgcagac aaagacagt tttacttgg 
        tttttggaa acctttaaa gcaaataat ccacagact 
        agattcaca aagtgcatc atggctgat aaagactta 
        ctagaaaaa acagttata aaagacaag ttcccagac 
        gctaatgtg aacatttgt atctttcac tcaatgcaa 
        atattcagc cgagcaatt acctgtgaa aaaatgagt 
        atcactcac gccgaaaga gttaagtcg ctgaaaatt 
        ttacaaaaa atggtttat tccgagaca gaagaagag 
        tttgaacag ttgcattac cttctaaaa atatcagct 
        ccaaaaccg gttactgaa tattttgac aaaaactgg 
        tatccagtt aaggatgaa tggtcattg tctaaaagt 
        ttcataaaa ggttgtttt caaaacacc acaaataat 
        catatcgaa aaaataaat gcgaaactc aagtccgtt 
        atcaataaa aacagctca ttagtacaa tttatgcat 
        tcctttttc accattatg tcatctctt gagagtgaa 
        catgatttt aaagccata tcccagtta caaaaaaga 
        ctcgtcacc tcgtactca ccagaaagc gacgaacaa 
        aaatatctg gatttttta accaacgaa gctggtaaa 
        atagtgata aaacaaatt gagttgagc aaaaaaatt 
        caagttcct tcagttgaa aacaatggt atcaacagt 
        acggaagga cgacaaaca tgtgatcca actaattgc 
        acttgtaat tttaaaact agtatgttg cttccttgc 
        agacacatt tttgcaatt cgaaagcgt ctggaattg 
        cctttgttt ttgaaagag cttgtgcat aagcgttgg 
        acactagat tattataaa atttgtcac aggcgtttg 
        gcacagtgc ccagtgttt gatacagat attgaaaca 
        tcggaagcg cctaatgta gttgtagca aataaaaac 
        aacacgaaa atatatgaa cagaaacga aaagaagtg 
        ttaagtgta atggagtcg ttggtgaat ttggtcgcc 
        atatccagc aatgattca tttgatcaa aaaatggat 
        aatttaaaa actcttgaa atgttgtgg aagcaaggc 
        agtaggaca ggaatagta ataatcaaa gaggaggat 
        gaaggcgag ttaccatta gagaatttg aacgaacaa 
        gtaataatc aaagaggag gatgaagca gatgaaggc 
        gaattacca ctagtcaat ttgaacgaa attgacgaa 
        ggcaaaagg acaagacca gtaatgatc aaagaggag 
        aatgaagca gatgaaagc gaatttcat ttggtgaat 
        tcaaacgaa ttagtaatg attaaagag gaagatgac 
        gctgatgaa gacgagtta ccattgaat ttgaacgaa 
        attgcccct gacgagtca aatgaagac accattatt 
        caagaaccc accatcagt gaggtagca agtattaaa 
        atatctcca aagattaaa gaaagagga cgtcctaga 
        ggtagcgaa ttaacaact gctggtctg ccaaaacgg 
        caaaagcta catgtgccc tttcccaaa cttacattg 
        gaacctaaa cagaaaaaa atattgggt tggtttgtt 
        tctgacgaa gttgtggcc ttggctatt aacgaaaga 
        ctgatcatt gaagagaag tatgtggaa acaagaccg 
        gaaaatatt cctcacgcg gttttacat gaaaatgta 
        gatatgaat atagttcgt aactttttt tctgatact 
        gcgtggagt gttgtagaa aatgtaatc gccctgaaa 
        agacagaac aaattgctg tgtaattag aaaattacg 
        caaaatcaa tctgattgt atcatacgt cccttatgc 
        cttttgtgg tggcatgtt aatggacaa cgttaacaa 
        agcacaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY