Status
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Gene information
General information
TC016092 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 1620015..1627819
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016092
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_11015

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016092 RA
      MRFKGLWEL ADLYPNCDV NVWLRSCIE EIPEPITGT 
      TTGTIPKWL RGCLLRNGP GSIKVGDDY FGHIFDSSA 
      LLHRFCIDE GGVTYQCRF VQSEVFKRN RAANRIVVT 
      EFGTKAVPD PCRTIFQRV ASVFNPKSE DIPDNSMIS 
      VYPFGDEVY AFGELPIIH KIDQETLET KERVDVRDF 
      VSIVHHTSH PHVMNDETV FNLGETIYP TGPYHTIVR 
      FPPGKNMVE GAEIVAGIP ARWPLHPTY MHTFGLTEN 
      YFVIVEQPL SVSVGGMIM NKIKNEPMK GSLRWYQHE 
      KTQFSLISR ETGQLAYKF YSEAFFYLH IINQYETCD 
      HVVVDICTY RNPEMLDCM YISAMKNMQ QNPNYAELF 
      RSKPLRFVL PLKPPETNS NLVQLSNTK CEAYYQPDG 
      EIYVIPEKL SDLGCETPR INYGQYLGK QYRYFYAIS 
      ADVDAANPG TMIKVDTVT KTAKTWCED NCYPSEPIF 
      VPRPNCAFE DDGLILAAM AWGREDTNH VGLIILDAE 
      SFTEVARTE FRTPGPVPK CLHGWFLPK
    • >TC016092 RB
      MASQSSNQK ADLYPNCDV NVWLRSCIE EIPEPITGT 
      TTGTIPKWL RGCLLRNGP GSIKVGDDY FGHIFDSSA 
      LLHRFCIDE GGVTYQCRF VQSEVFKRN RAANRIVVT 
      EFGTKAVPD PCRTIFQRV ASVFNPKSE DIPDNSMIS 
      VYPFGDEVY AFGELPIIH KIDQETLET KERVDVRDF 
      VSIVHHTSH PHVMNDETV FNLGETIYP TGPYHTIVR 
      FPPGKNMVE GAEIVAGIP ARWPLHPTY MHTFGLTEN 
      YFVIVEQPL SVSVGGMIM NKIKNEPMK GSLRWYQHE 
      KTQFSLISR ETGQLAYKF YSEAFFYLH IINQYETCD 
      HVVVDICTY RNPEMLDCM YISAMKNMQ QNPNYAELF 
      RSKPLRFVL PLKPPETNS NLVQLSNTK CEAYYQPDG 
      EIYVIPEKL SDLGCETPR INYGQYLGK QYRYFYAIS 
      ADVDAANPG TMIKVDTVT KTAKTWCED NCYPSEPIF 
      VPRPNCAFE DDGLILAAM AWGREDTNH VGLIILDAE 
      SFTEVARTE FRTPGPVPK CLHGWFLPK
    CDS sequence
    • >TC016092 RA
      atgcgtttt aagggactt tgggaattg gcagacttg 
      tacccaaac tgtgacgtc aatgtgtgg ttacggtcc 
      tgtattgaa gaaatccca gaacccatc accggtacc 
      accactggt accattcca aaatggttg cggggttgc 
      cttctacgt aatggaccc ggttcgatc aaagtcggt 
      gatgactat ttcggacat atttttgac agttcggcc 
      ctcctccac cggttttgc atcgacgag ggcggggtt 
      acttatcag tgccgtttc gtccaatca gaagtgttc 
      aaacggaac cgagcagcc aatcggatt gtcgtcacc 
      gaatttggc actaaagcc gtccctgat ccgtgccgt 
      accatcttc caaagagtt gcttcagtt tttaatcca 
      aaatctgag gatattccg gataattca atgatttcg 
      gtttatccg ttcggggac gaagtttat gcattcggg 
      gaactcccg ataattcac aaaattgat caagagact 
      ttggaaacg aaagaacgg gttgacgtt cgcgatttt 
      gtttcaatt gtccatcac acctctcat ccgcatgtt 
      atgaacgat gaaactgtt ttcaacttg ggggaaaca 
      atatacccg actggaccc tatcacacg attgttagg 
      tttccaccg gggaaaaac atggttgaa ggtgcagaa 
      atcgtagcg ggaatcccc gccagatgg ccactgcac 
      cctacatac atgcacact tttggcctg acggaaaat 
      tattttgtc attgttgaa caacctcta agtgtttct 
      gttggtggt atgattatg aataagatt aaaaatgag 
      ccgatgaaa gggagtctt cggtggtac caacacgaa 
      aaaacacaa ttcagttta ataagtcgt gaaactggc 
      caactggct tacaaattt tactcagaa gcgttcttt 
      tatttacac attatcaac caatacgag acttgcgat 
      cacgtggtt gttgacatt tgcacgtac cgcaacccc 
      gaaatgctg gactgtatg tacatcagt gcaatgaag 
      aacatgcaa caaaacccg aactatgcc gaactgttc 
      cgttcaaaa ccgctccgt ttcgtactt cccctaaaa 
      ccgccggaa acaaattcg aacctagtc caactgtca 
      aacactaaa tgcgaagcg tattaccaa cctgacggc 
      gaaatttac gtaataccg gaaaaatta tcagatttg 
      ggatgtgaa acacccaga atcaactat gggcaatat 
      ttgggaaag cagtatcga tatttttac gctattagc 
      gctgacgtc gacgcggca aacccggga actatgatc 
      aaagttgat accgtaaca aaaaccgcc aaaacttgg 
      tgtgaggat aattgttac cccagtgaa cccattttt 
      gtaccacgg cccaattgt gcctttgag gacgatggg 
      ttgatttta gccgccatg gcatggggt agagaagac 
      acaaaccat gtcggtttg attattttg gatgcagaa 
      agtttcaca gaagtggca agaactgag tttaggacg 
      cctggacct gtacccaaa tgtttacat ggctggttt 
      ttgccgaaa taa
    • >TC016092 RB
      atggcatca cagagttca aaccaaaaa gcagacttg 
      tacccaaac tgtgacgtc aatgtgtgg ttacggtcc 
      tgtattgaa gaaatccca gaacccatc accggtacc 
      accactggt accattcca aaatggttg cggggttgc 
      cttctacgt aatggaccc ggttcgatc aaagtcggt 
      gatgactat ttcggacat atttttgac agttcggcc 
      ctcctccac cggttttgc atcgacgag ggcggggtt 
      acttatcag tgccgtttc gtccaatca gaagtgttc 
      aaacggaac cgagcagcc aatcggatt gtcgtcacc 
      gaatttggc actaaagcc gtccctgat ccgtgccgt 
      accatcttc caaagagtt gcttcagtt tttaatcca 
      aaatctgag gatattccg gataattca atgatttcg 
      gtttatccg ttcggggac gaagtttat gcattcggg 
      gaactcccg ataattcac aaaattgat caagagact 
      ttggaaacg aaagaacgg gttgacgtt cgcgatttt 
      gtttcaatt gtccatcac acctctcat ccgcatgtt 
      atgaacgat gaaactgtt ttcaacttg ggggaaaca 
      atatacccg actggaccc tatcacacg attgttagg 
      tttccaccg gggaaaaac atggttgaa ggtgcagaa 
      atcgtagcg ggaatcccc gccagatgg ccactgcac 
      cctacatac atgcacact tttggcctg acggaaaat 
      tattttgtc attgttgaa caacctcta agtgtttct 
      gttggtggt atgattatg aataagatt aaaaatgag 
      ccgatgaaa gggagtctt cggtggtac caacacgaa 
      aaaacacaa ttcagttta ataagtcgt gaaactggc 
      caactggct tacaaattt tactcagaa gcgttcttt 
      tatttacac attatcaac caatacgag acttgcgat 
      cacgtggtt gttgacatt tgcacgtac cgcaacccc 
      gaaatgctg gactgtatg tacatcagt gcaatgaag 
      aacatgcaa caaaacccg aactatgcc gaactgttc 
      cgttcaaaa ccgctccgt ttcgtactt cccctaaaa 
      ccgccggaa acaaattcg aacctagtc caactgtca 
      aacactaaa tgcgaagcg tattaccaa cctgacggc 
      gaaatttac gtaataccg gaaaaatta tcagatttg 
      ggatgtgaa acacccaga atcaactat gggcaatat 
      ttgggaaag cagtatcga tatttttac gctattagc 
      gctgacgtc gacgcggca aacccggga actatgatc 
      aaagttgat accgtaaca aaaaccgcc aaaacttgg 
      tgtgaggat aattgttac cccagtgaa cccattttt 
      gtaccacgg cccaattgt gcctttgag gacgatggg 
      ttgatttta gccgccatg gcatggggt agagaagac 
      acaaaccat gtcggtttg attattttg gatgcagaa 
      agtttcaca gaagtggca agaactgag tttaggacg 
      cctggacct gtacccaaa tgtttacat ggctggttt 
      ttgccgaaa taa
    mRNA sequence
    • >TC016092 RA
      gtgggtgga aaaaatatg gccagcgtg attaaatca 
      agtgtggga tgctgaccc acatcgggg gctgatcga 
      tgcgtttta agggacttt gggaattgg cagacttgt 
      acccaaact gtgacgtca atgtgtggt tacggtcct 
      gtattgaag aaatcccag aacccatca ccggtacca 
      ccactggta ccattccaa aatggttgc ggggttgcc 
      ttctacgta atggacccg gttcgatca aagtcggtg 
      atgactatt tcggacata tttttgaca gttcggccc 
      tcctccacc ggttttgca tcgacgagg gcggggtta 
      cttatcagt gccgtttcg tccaatcag aagtgttca 
      aacggaacc gagcagcca atcggattg tcgtcaccg 
      aatttggca ctaaagccg tccctgatc cgtgccgta 
      ccatcttcc aaagagttg cttcagttt ttaatccaa 
      aatctgagg atattccgg ataattcaa tgatttcgg 
      tttatccgt tcggggacg aagtttatg cattcgggg 
      aactcccga taattcaca aaattgatc aagagactt 
      tggaaacga aagaacggg ttgacgttc gcgattttg 
      tttcaattg tccatcaca cctctcatc cgcatgtta 
      tgaacgatg aaactgttt tcaacttgg gggaaacaa 
      tatacccga ctggaccct atcacacga ttgttaggt 
      ttccaccgg ggaaaaaca tggttgaag gtgcagaaa 
      tcgtagcgg gaatccccg ccagatggc cactgcacc 
      ctacataca tgcacactt ttggcctga cggaaaatt 
      attttgtca ttgttgaac aacctctaa gtgtttctg 
      ttggtggta tgattatga ataagatta aaaatgagc 
      cgatgaaag ggagtcttc ggtggtacc aacacgaaa 
      aaacacaat tcagtttaa taagtcgtg aaactggcc 
      aactggctt acaaatttt actcagaag cgttctttt 
      atttacaca ttatcaacc aatacgaga cttgcgatc 
      acgtggttg ttgacattt gcacgtacc gcaaccccg 
      aaatgctgg actgtatgt acatcagtg caatgaaga 
      acatgcaac aaaacccga actatgccg aactgttcc 
      gttcaaaac cgctccgtt tcgtacttc ccctaaaac 
      cgccggaaa caaattcga acctagtcc aactgtcaa 
      acactaaat gcgaagcgt attaccaac ctgacggcg 
      aaatttacg taataccgg aaaaattat cagatttgg 
      gatgtgaaa cacccagaa tcaactatg ggcaatatt 
      tgggaaagc agtatcgat atttttacg ctattagcg 
      ctgacgtcg acgcggcaa acccgggaa ctatgatca 
      aagttgata ccgtaacaa aaaccgcca aaacttggt 
      gtgaggata attgttacc ccagtgaac ccatttttg 
      taccacggc ccaattgtg cctttgagg acgatgggt 
      tgattttag ccgccatgg catggggta gagaagaca 
      caaaccatg tcggtttga ttattttgg atgcagaaa 
      gtttcacag aagtggcaa gaactgagt ttaggacgc 
      ctggacctg tacccaaat gtttacatg gctggtttt 
      tgccgaaat aaatgaatc atactgtag attttgttg 
      tattcaggt gtgacaaag atagcatga ttgggagaa 
      acgtcaaga aaactagga aatgtcaaa atctttaca 
      tttttattt tttttattg tatcaaaaa ggaataaag 
      atgtgttgg tggt
    • >TC016092 RB
      aaacgatgg catcacaga gttcaaacc aaaaagcag 
      acttgtacc caaactgtg acgtcaatg tgtggttac 
      ggtcctgta ttgaagaaa tcccagaac ccatcaccg 
      gtaccacca ctggtacca ttccaaaat ggttgcggg 
      gttgccttc tacgtaatg gacccggtt cgatcaaag 
      tcggtgatg actatttcg gacatattt ttgacagtt 
      cggccctcc tccaccggt tttgcatcg acgagggcg 
      gggttactt atcagtgcc gtttcgtcc aatcagaag 
      tgttcaaac ggaaccgag cagccaatc ggattgtcg 
      tcaccgaat ttggcacta aagccgtcc ctgatccgt 
      gccgtacca tcttccaaa gagttgctt cagttttta 
      atccaaaat ctgaggata ttccggata attcaatga 
      tttcggttt atccgttcg gggacgaag tttatgcat 
      tcggggaac tcccgataa ttcacaaaa ttgatcaag 
      agactttgg aaacgaaag aacgggttg acgttcgcg 
      attttgttt caattgtcc atcacacct ctcatccgc 
      atgttatga acgatgaaa ctgttttca acttggggg 
      aaacaatat acccgactg gaccctatc acacgattg 
      ttaggtttc caccgggga aaaacatgg ttgaaggtg 
      cagaaatcg tagcgggaa tccccgcca gatggccac 
      tgcacccta catacatgc acacttttg gcctgacgg 
      aaaattatt ttgtcattg ttgaacaac ctctaagtg 
      tttctgttg gtggtatga ttatgaata agattaaaa 
      atgagccga tgaaaggga gtcttcggt ggtaccaac 
      acgaaaaaa cacaattca gtttaataa gtcgtgaaa 
      ctggccaac tggcttaca aattttact cagaagcgt 
      tcttttatt tacacatta tcaaccaat acgagactt 
      gcgatcacg tggttgttg acatttgca cgtaccgca 
      accccgaaa tgctggact gtatgtaca tcagtgcaa 
      tgaagaaca tgcaacaaa acccgaact atgccgaac 
      tgttccgtt caaaaccgc tccgtttcg tacttcccc 
      taaaaccgc cggaaacaa attcgaacc tagtccaac 
      tgtcaaaca ctaaatgcg aagcgtatt accaacctg 
      acggcgaaa tttacgtaa taccggaaa aattatcag 
      atttgggat gtgaaacac ccagaatca actatgggc 
      aatatttgg gaaagcagt atcgatatt tttacgcta 
      ttagcgctg acgtcgacg cggcaaacc cgggaacta 
      tgatcaaag ttgataccg taacaaaaa ccgccaaaa 
      cttggtgtg aggataatt gttacccca gtgaaccca 
      tttttgtac cacggccca attgtgcct ttgaggacg 
      atgggttga ttttagccg ccatggcat ggggtagag 
      aagacacaa accatgtcg gtttgatta ttttggatg 
      cagaaagtt tcacagaag tggcaagaa ctgagttta 
      ggacgcctg gacctgtac ccaaatgtt tacatggct 
      ggtttttgc cgaaataaa tgaatcata ctgtagatt 
      ttgttgtat tcaggtgtg acaaagata gcatgattg 
      ggagaaacg tcaagaaaa ctaggaaat gtcaaaatc 
      tttacattt ttatttttt ttattgtat caaaaagga 
      ataaagatg tgttggtgg t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY