Status
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Gene information
General information
TC008818 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10371509..10387256
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008818
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_11013

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008818 RA
      MNVESSGKG QNNFNENHA EPKNNSVAN APPPTPLNK 
      TKLTTGTVQ GPMIKKDKR HSSSRFNIT KNRELEMLP 
      SLKDAAPAD REELFIQKL RQCCVLFDF ASDPLSDLK 
      WKEVKRAAL HEMVEYVTQ QKGVITEAI YPEAVNMFA 
      VNLFRTLPP SSNPNGAEF DPEEDEPTL EAAWPHLQL 
      VYELFLRVL EAPDFQPNI AKRYIDQKF VLQLLDLFD 
      SEDPRERDL LKTTLHRIY GKFLGLRAF IRKQINNVF 
      YRFIYETEH HNGIAELLE ILGSIINGF ALPLKEEHK 
      VFLLRVLMP LHKVKSLSV YHPQLAYCV VQFLEKDPS 
      LTQPVIRSL LKYWPKTHS PKEVMFLNE LEEILDVIE 
      PGEFQKVMV PLFKQLAKC VSSPHFQVA ERALYYWNN 
      EYVMSLVSE NATEILPLM FPSLYKNSK SHWNKTIHG 
      LIYNALKLF MEMNQKLFD ECTQLYREE RRKEREREN 
      QRMELWIRV ESLAAQKPE YELIKAQNP EIKSIITSP 
      DPDDEVTLD NIEQQAQET KKTRNTNKP LVRRKSELP 
      QDQYTVKAL NDHKRADEY LTTPPDVNK C
    • >TC008818 RB
      MVHSTFAQG HVMGGRDSS PLGIPKSPS FHSGLDLVA 
      SASVDSLVA AQTAAKHLD NDTPARPDD GKMPGQRRN 
      TTFRGMQDA ELLPQLEDA APADREELF IQKLRQCCV 
      LFDFASDPL SDLKWKEVK RAALHEMVE YVTQQKGVI 
      TEAIYPEAV NMFAVNLFR TLPPSSNPN GAEFDPEED 
      EPTLEAAWP HLQLVYELF LRVLEAPDF QPNIAKRYI 
      DQKFVLQLL DLFDSEDPR ERDLLKTTL HRIYGKFLG 
      LRAFIRKQI NNVFYRFIY ETEHHNGIA ELLEILGSI 
      INGFALPLK EEHKVFLLR VLMPLHKVK SLSVYHPQL 
      AYCVVQFLE KDPSLTQPV IRSLLKYWP KTHSPKEVM 
      FLNELEEIL DVIEPGEFQ KVMVPLFKQ LAKCVSSPH 
      FQVAERALY YWNNEYVMS LVSENATEI LPLMFPSLY 
      KNSKSHWNK TIHGLIYNA LKLFMEMNQ KLFDECTQL 
      YREERRKER ERENQRMEL WIRVESLAA QKPEYELIK 
      AQNPEIKSI ITSPDPDDE VTLDNIEQQ AQETKKTRN 
      TNKPLVRRK SELPQDQYT VKALNDHKR ADEYLTTPP 
      DVNKC
    CDS sequence
    • >TC008818 RA
      atgaacgtc gagtcgtct ggcaagggg caaaacaac 
      ttcaacgag aatcatgcg gagccaaag aataactcc 
      gtggcaaat gcgcccccg cctacgccc ctcaacaaa 
      accaagctg acgacggga accgttcaa gggcccatg 
      atcaaaaag gacaagcga cattccagc tccaggttc 
      aatatcacc aaaaacagg gaactggag atgcttccg 
      tcgttaaaa gacgccgct ccggccgat cgagaagag 
      ctcttcatc caaaaactc cgtcaatgt tgcgttctt 
      tttgatttt gcttccgac cccttgtct gatttaaaa 
      tggaaggaa gtgaaacga gccgccctt cacgaaatg 
      gtggaatat gtaacgcag cagaaaggc gtcatcacc 
      gaagctatt tatccagag gccgtcaat atgttcgcc 
      gtgaacctg ttccgcacg ttgcccccg tcgtcgaac 
      ccaaacggt gccgaattc gacccagaa gaggacgag 
      ccaacctta gaggccgct tggccccac ctccagctg 
      gtctacgaa ttgtttttg cgcgtgctg gaggcgccc 
      gatttccag ccgaacatt gccaaacgt tacatagac 
      caaaagttc gtcctccag ctgctcgat ctgttcgat 
      tcggaagat ccccgcgaa cgggatctt ctgaaaacg 
      actcttcat cggatttac ggcaagttc ctcggactt 
      cgcgccttc attcgcaaa caaatcaac aacgtgttt 
      tatcgcttc atctacgaa accgagcac cacaacggc 
      atcgcagag cttcttgaa atcctcgga tcgataatc 
      aacggtttc gcattgccg ctgaaggaa gaacacaaa 
      gtgtttctt ttgcgagtt ttgatgccg ctgcataaa 
      gtcaagtcg ttgtcggtg taccatccg cagttggcc 
      tactgtgtt gtgcagttt ttggagaag gacccgtcg 
      ttgacccaa ccagtgatt aggagtctg ttgaagtac 
      tggcccaag acgcatagt cccaaagaa gtgatgttt 
      ctgaacgaa ctggaggag atcctcgac gttatagaa 
      ccaggggaa ttccagaaa gtcatggtt ccgttgttc 
      aaacaactg gcgaagtgt gtcagctcg ccgcatttt 
      caagtcgcc gagagggcg ctctattac tggaataat 
      gaatatgtg atgtctctg gtgtcggaa aacgccacc 
      gagattcta ccactgatg ttccccagt ctttacaaa 
      aactcgaaa agtcactgg aacaagact atacacggc 
      ctgatttac aatgcactt aagttgttt atggaaatg 
      aatcaaaaa ttgtttgac gagtgtaca caactatat 
      cgtgaggag agacggaaa gagcgcgag cgtgaaaac 
      caacgtatg gaactatgg atcagagtg gagtcttta 
      gcagctcag aaacccgaa tacgagctt attaaagca 
      caaaatcca gaaatcaag agcatcatc acgagtcct 
      gatcccgac gatgaagtc actttggat aacatagaa 
      caacaagcc caggagaca aagaagact cgcaataca 
      aacaagccg ttagtccga cgtaaatcg gaattacca 
      caagatcaa tacaccgtc aaagcactg aacgatcac 
      aaacgagca gacgagtat ctcacgacg ccaccggat 
      gtcaacaag tgctag
    • >TC008818 RB
      atggttcac tcaactttc gcgcaaggt catgttatg 
      ggaggtcgg gattcgtct ccattaggc atacccaaa 
      tcgccgtcg tttcacagc ggcttggat ttggtggcg 
      tcggccagc gtcgattcg ctcgtcgcg gcgcaaacc 
      gctgccaag catctcgac aacgacacg ccagcaagg 
      cccgacgac gggaaaatg ccggggcag aggaggaac 
      accaccttc agaggcatg caggacgca gagctgctg 
      ccccagtta gaagacgcc gctccggcc gatcgagaa 
      gagctcttc atccaaaaa ctccgtcaa tgttgcgtt 
      ctttttgat tttgcttcc gaccccttg tctgattta 
      aaatggaag gaagtgaaa cgagccgcc cttcacgaa 
      atggtggaa tatgtaacg cagcagaaa ggcgtcatc 
      accgaagct atttatcca gaggccgtc aatatgttc 
      gccgtgaac ctgttccgc acgttgccc ccgtcgtcg 
      aacccaaac ggtgccgaa ttcgaccca gaagaggac 
      gagccaacc ttagaggcc gcttggccc cacctccag 
      ctggtctac gaattgttt ttgcgcgtg ctggaggcg 
      cccgatttc cagccgaac attgccaaa cgttacata 
      gaccaaaag ttcgtcctc cagctgctc gatctgttc 
      gattcggaa gatccccgc gaacgggat cttctgaaa 
      acgactctt catcggatt tacggcaag ttcctcgga 
      cttcgcgcc ttcattcgc aaacaaatc aacaacgtg 
      ttttatcgc ttcatctac gaaaccgag caccacaac 
      ggcatcgca gagcttctt gaaatcctc ggatcgata 
      atcaacggt ttcgcattg ccgctgaag gaagaacac 
      aaagtgttt cttttgcga gttttgatg ccgctgcat 
      aaagtcaag tcgttgtcg gtgtaccat ccgcagttg 
      gcctactgt gttgtgcag tttttggag aaggacccg 
      tcgttgacc caaccagtg attaggagt ctgttgaag 
      tactggccc aagacgcat agtcccaaa gaagtgatg 
      tttctgaac gaactggag gagatcctc gacgttata 
      gaaccaggg gaattccag aaagtcatg gttccgttg 
      ttcaaacaa ctggcgaag tgtgtcagc tcgccgcat 
      tttcaagtc gccgagagg gcgctctat tactggaat 
      aatgaatat gtgatgtct ctggtgtcg gaaaacgcc 
      accgagatt ctaccactg atgttcccc agtctttac 
      aaaaactcg aaaagtcac tggaacaag actatacac 
      ggcctgatt tacaatgca cttaagttg tttatggaa 
      atgaatcaa aaattgttt gacgagtgt acacaacta 
      tatcgtgag gagagacgg aaagagcgc gagcgtgaa 
      aaccaacgt atggaacta tggatcaga gtggagtct 
      ttagcagct cagaaaccc gaatacgag cttattaaa 
      gcacaaaat ccagaaatc aagagcatc atcacgagt 
      cctgatccc gacgatgaa gtcactttg gataacata 
      gaacaacaa gcccaggag acaaagaag actcgcaat 
      acaaacaag ccgttagtc cgacgtaaa tcggaatta 
      ccacaagat caatacacc gtcaaagca ctgaacgat 
      cacaaacga gcagacgag tatctcacg acgccaccg 
      gatgtcaac aagtgctag
    mRNA sequence
    • >TC008818 RA
      gtttaattg aaaaaaata aaattggaa caattaaaa 
      gaagtcgat tgaagatga cttaaactg ggccacgtg 
      acgtccggc gtccaatga gcggtcgga tcgtcgtca 
      ttcggtagt cgttcgccg ccagtagat gtcgcatgt 
      gtcgttgat gcagtcctt cgtgtgtct tttaaccct 
      agtgcattt cctttaatt ttaatgact ttttggctg 
      ttatttggt tttgttcgt cgttcagca cctccgcaa 
      gtgtccgcc tggtagtcc ttcgagcga agtgtacac 
      gttggaggt tgacggcgc ctggagtgt cggtaaacg 
      tgatgaaaa gtctgacgc cgtggctgt gaatgtttt 
      cttaattta gtgtcttga tcgcgaacc gtcatcgtc 
      gtcaacgac gtcaacatg gtctgtatc cggcgttgt 
      cttccgcaa attgtcgac aattaggtt agtcggcgc 
      aacaatcaa gtttgtcca tgttgcagg acgcaagga 
      tcatgaacg tcgagtcgt ctggcaagg ggcaaaaca 
      acttcaacg agaatcatg cggagccaa agaataact 
      ccgtggcaa atgcgcccc cgcctacgc ccctcaaca 
      aaaccaagc tgacgacgg gaaccgttc aagggccca 
      tgatcaaaa aggacaagc gacattcca gctccaggt 
      tcaatatca ccaaaaaca gggaactgg agatgcttc 
      cgtcgttaa aagacgccg ctccggccg atcgagaag 
      agctcttca tccaaaaac tccgtcaat gttgcgttc 
      tttttgatt ttgcttccg accccttgt ctgatttaa 
      aatggaagg aagtgaaac gagccgccc ttcacgaaa 
      tggtggaat atgtaacgc agcagaaag gcgtcatca 
      ccgaagcta tttatccag aggccgtca atatgttcg 
      ccgtgaacc tgttccgca cgttgcccc cgtcgtcga 
      acccaaacg gtgccgaat tcgacccag aagaggacg 
      agccaacct tagaggccg cttggcccc acctccagc 
      tggtctacg aattgtttt tgcgcgtgc tggaggcgc 
      ccgatttcc agccgaaca ttgccaaac gttacatag 
      accaaaagt tcgtcctcc agctgctcg atctgttcg 
      attcggaag atccccgcg aacgggatc ttctgaaaa 
      cgactcttc atcggattt acggcaagt tcctcggac 
      ttcgcgcct tcattcgca aacaaatca acaacgtgt 
      tttatcgct tcatctacg aaaccgagc accacaacg 
      gcatcgcag agcttcttg aaatcctcg gatcgataa 
      tcaacggtt tcgcattgc cgctgaagg aagaacaca 
      aagtgtttc ttttgcgag ttttgatgc cgctgcata 
      aagtcaagt cgttgtcgg tgtaccatc cgcagttgg 
      cctactgtg ttgtgcagt ttttggaga aggacccgt 
      cgttgaccc aaccagtga ttaggagtc tgttgaagt 
      actggccca agacgcata gtcccaaag aagtgatgt 
      ttctgaacg aactggagg agatcctcg acgttatag 
      aaccagggg aattccaga aagtcatgg ttccgttgt 
      tcaaacaac tggcgaagt gtgtcagct cgccgcatt 
      ttcaagtcg ccgagaggg cgctctatt actggaata 
      atgaatatg tgatgtctc tggtgtcgg aaaacgcca 
      ccgagattc taccactga tgttcccca gtctttaca 
      aaaactcga aaagtcact ggaacaaga ctatacacg 
      gcctgattt acaatgcac ttaagttgt ttatggaaa 
      tgaatcaaa aattgtttg acgagtgta cacaactat 
      atcgtgagg agagacgga aagagcgcg agcgtgaaa 
      accaacgta tggaactat ggatcagag tggagtctt 
      tagcagctc agaaacccg aatacgagc ttattaaag 
      cacaaaatc cagaaatca agagcatca tcacgagtc 
      ctgatcccg acgatgaag tcactttgg ataacatag 
      aacaacaag cccaggaga caaagaaga ctcgcaata 
      caaacaagc cgttagtcc gacgtaaat cggaattac 
      cacaagatc aatacaccg tcaaagcac tgaacgatc 
      acaaacgag cagacgagt atctcacga cgccaccgg 
      atgtcaaca agtgctagt ccactcaaa acacctttt 
      cttagtgag cttagtgcc aacaggtta tgttatgcg 
      acctaggga accaccggg tattttcat tcatttcaa 
      ttgatttga ggtgacaag tattgattt tatgctagg 
      gcgatgtga ttttaggtc tttttttat aaagattga 
      tgtttttta ttggtttga gagaaaagt caaaatatt 
      tttagatac cgtagttga tagacaact ttcgagtac 
      aattttttc gcgctaagg ttattattt ctatgcaaa 
      tattctatc tagatccat agttgtaaa tgactgcat 
      gaaaggatt tatgtgttg ttagccgtt actaaatta 
      gcatgtggc cttttataa aataatata tttaagatg 
      tttataacg atatcgagt tggtcgttc ttaccaaac 
      gctgtttct tttttaaca cttttctct ctgtttatt 
      tattctttt tataatcga acaatcggg ggttagttg 
      aaaaagagt gtacagaca ttatttacg tatttagaa 
      attaaatgt gtggcactt gctttgaat gtgttatta 
      aacaaaaac gaatgagca tattgttag ttaactgat 
      aaaagaaag tgtaagtct agtgttgta actcgaaat 
      acattcaat aattagctg taaggatat attttttat 
      gtttcatga tggaaggaa agtgtacat acggatatg 
      gagttgata ttatgtctt agacagtat ttttttatt 
      gtacagctt tgctttgtc aatgatatt gatataaat 
      tacatttct ttaattcac tcaaatgtt catttttgc 
      caaacg
    • >TC008818 RB
      cttcacccc ttatcagta cagtttggc ggagagccc 
      gaccgcgac aaacattcc gcgatgatg acttgacgc 
      attgcgtct tcttcatct cgccaataa ttagctcaa 
      ttcgcgaat tgcccacgg taaggtcgt gcgtgtgtg 
      cgtgcgtgc gtggagtgc caccacatt tcaaaaacg 
      ggagttgag agcttcacc atggttcac tcaactttc 
      gcgcaaggt catgttatg ggaggtcgg gattcgtct 
      ccattaggc atacccaaa tcgccgtcg tttcacagc 
      ggcttggat ttggtggcg tcggccagc gtcgattcg 
      ctcgtcgcg gcgcaaacc gctgccaag catctcgac 
      aacgacacg ccagcaagg cccgacgac gggaaaatg 
      ccggggcag aggaggaac accaccttc agaggcatg 
      caggacgca gagctgctg ccccagtta gaagacgcc 
      gctccggcc gatcgagaa gagctcttc atccaaaaa 
      ctccgtcaa tgttgcgtt ctttttgat tttgcttcc 
      gaccccttg tctgattta aaatggaag gaagtgaaa 
      cgagccgcc cttcacgaa atggtggaa tatgtaacg 
      cagcagaaa ggcgtcatc accgaagct atttatcca 
      gaggccgtc aatatgttc gccgtgaac ctgttccgc 
      acgttgccc ccgtcgtcg aacccaaac ggtgccgaa 
      ttcgaccca gaagaggac gagccaacc ttagaggcc 
      gcttggccc cacctccag ctggtctac gaattgttt 
      ttgcgcgtg ctggaggcg cccgatttc cagccgaac 
      attgccaaa cgttacata gaccaaaag ttcgtcctc 
      cagctgctc gatctgttc gattcggaa gatccccgc 
      gaacgggat cttctgaaa acgactctt catcggatt 
      tacggcaag ttcctcgga cttcgcgcc ttcattcgc 
      aaacaaatc aacaacgtg ttttatcgc ttcatctac 
      gaaaccgag caccacaac ggcatcgca gagcttctt 
      gaaatcctc ggatcgata atcaacggt ttcgcattg 
      ccgctgaag gaagaacac aaagtgttt cttttgcga 
      gttttgatg ccgctgcat aaagtcaag tcgttgtcg 
      gtgtaccat ccgcagttg gcctactgt gttgtgcag 
      tttttggag aaggacccg tcgttgacc caaccagtg 
      attaggagt ctgttgaag tactggccc aagacgcat 
      agtcccaaa gaagtgatg tttctgaac gaactggag 
      gagatcctc gacgttata gaaccaggg gaattccag 
      aaagtcatg gttccgttg ttcaaacaa ctggcgaag 
      tgtgtcagc tcgccgcat tttcaagtc gccgagagg 
      gcgctctat tactggaat aatgaatat gtgatgtct 
      ctggtgtcg gaaaacgcc accgagatt ctaccactg 
      atgttcccc agtctttac aaaaactcg aaaagtcac 
      tggaacaag actatacac ggcctgatt tacaatgca 
      cttaagttg tttatggaa atgaatcaa aaattgttt 
      gacgagtgt acacaacta tatcgtgag gagagacgg 
      aaagagcgc gagcgtgaa aaccaacgt atggaacta 
      tggatcaga gtggagtct ttagcagct cagaaaccc 
      gaatacgag cttattaaa gcacaaaat ccagaaatc 
      aagagcatc atcacgagt cctgatccc gacgatgaa 
      gtcactttg gataacata gaacaacaa gcccaggag 
      acaaagaag actcgcaat acaaacaag ccgttagtc 
      cgacgtaaa tcggaatta ccacaagat caatacacc 
      gtcaaagca ctgaacgat cacaaacga gcagacgag 
      tatctcacg acgccaccg gatgtcaac aagtgctag 
      tccactcaa aacaccttt tcttagtga gcttagtgc 
      caacaggtt atgttatgc gacctaggg aaccaccgg 
      gtattttca ttcatttca attgatttg aggtgacaa 
      gtattgatt ttatgctag ggcgatgtg attttaggt 
      cttttttta taaagattg atgtttttt attggtttg 
      agagaaaag tcaaaatat ttttagata ccgtagttg 
      atagacaac tttcgagta caatttttt cgcgctaag 
      gttattatt tctatgcaa atattctat ctagatcca 
      tagttgtaa atgactgca tgaaaggat ttatgtgtt 
      gttagccgt tactaaatt agcatgtgg ccttttata 
      aaataatat atttaagat gtttataac gatatcgag 
      ttggtcgtt cttaccaaa cgctgtttc ttttttaac 
      acttttctc tctgtttat ttattcttt ttataatcg 
      aacaatcgg gggttagtt gaaaaagag tgtacagac 
      attatttac gtatttaga aattaaatg tgtggcact 
      tgctttgaa tgtgttatt aaacaaaaa cgaatgagc 
      atattgtta gttaactga taaaagaaa gtgtaagtc 
      tagtgttgt aactcgaaa tacattcaa taattagct 
      gtaaggata tatttttta tgtttcatg atggaagga 
      aagtgtaca tacggatat ggagttgat attatgtct 
      tagacagta tttttttat tgtacagct ttgctttgt 
      caatgatat tgatataaa ttacatttc tttaattca 
      ctcaaatgt tcatttttg ccaaacg
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