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Gene information
General information
TC001773 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7754702..7758736
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001773
    iBeetle screen iB_11011

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001773 RA
      MSQKVEKMS STVYVVFLS LLLDLLAFT MILPLLPSL 
      LEYYKSNDS TGLYAWLSR RIQFFQELV GAPEKYNSV 
      LFGGFLGSM FSFLQFVVS PIVGGVSDV VGRKKVMVT 
      CLIGISTSY VLWCLSSNL VLFILARFI GGISKGNVS 
      LSMAIIADV STMRTRGKG MAFVGIAFS LGFIVGPLI 
      GALFAVWAK KKVGDWFIV PALFALLLS LADLIFFIF 
      CFKETLPEE KRAKSVKTS LNSAKSFIN WKDLFQFKA 
      VTGLKSSDM FELRRLGRV YFVYLFIYS GLEFTLTFL 
      THHIFNYNS MQQGWMFFS IGVTMALMQ GGYVRRISP 
      QKIRPTAIC GLWIIVPSF VCVGLAQGP LLLYFGLFL 
      FAVSTAMVV PCIMTMASE HGKESQKGT VMGIFRSLG 
      ALARALGPI FASIAFWSV GSRITYLCG AFGLLWPVL 
      TLTKKL
    CDS sequence
    • >TC001773 RA
      atgtcgcag aaagttgaa aaaatgagt tccactgtt 
      tacgtagtt tttctttcc cttttgttg gatttattg 
      gcgtttact atgatcctg ccattgttg ccttcgctg 
      ctcgagtat tacaagagt aacgacagc acggggctg 
      tacgcatgg ctctcacgg cgtattcag ttcttccaa 
      gagctggtc ggggccccc gagaagtac aattcggtc 
      ctttttggt ggttttctc gggtccatg ttcagcttt 
      ttgcagttt gttgtctcc cccatcgta gggggagtc 
      tccgacgtg gtcgggagg aagaaagtc atggtcact 
      tgtctgatt gggatatca acatcgtac gttctgtgg 
      tgcttgtcg agtaattta gttttgttt attttggcg 
      cggtttatt ggcggaatc agcaaaggc aacgtttcc 
      ttaagcatg gccataata gccgacgtc tcgacgatg 
      cgaacgcgg ggaaaaggc atggctttc gtcggcatt 
      gccttttcc ctgggcttc atcgttggc cccctgata 
      ggcgccctg ttcgcggtt tgggcgaag aaaaaagtg 
      ggcgattgg ttcatagtt cctgcactg ttcgcactt 
      ttgttgtcg cttgctgac ttgatcttt ttcattttt 
      tgtttcaag gagacgtta ccagaggaa aaacgtgcc 
      aaaagcgtg aaaacgagt ctaaacagt gccaaatcg 
      ttcatcaac tggaaggac ttgttccag tttaaggcc 
      gtaacgggc ttgaaatcg tccgatatg ttcgaattg 
      aggcgtctg ggacgcgtc tatttcgtc tatttgttc 
      atctacagc ggtctcgag ttcactctg acttttttg 
      acgcatcat atttttaat tataattca atgcaacaa 
      gggtggatg tttttcagt attggggtc acaatggcg 
      ctcatgcaa gggggatac gtgcgccga atctcgccc 
      caaaaaatc agacccacc gctatttgc ggactgtgg 
      attatagtg ccttcgttt gtctgcgtg gggctggcc 
      caagggccc cttttgctc tacttcgga ctgtttttg 
      ttcgcagtt tcgactgcg atggtggtt ccgtgcatc 
      atgacgatg gcgtcggag cacggcaaa gagagccag 
      aagggcaca gtcatgggc attttcagg tccctgggg 
      gccctagcc cgggccttg ggcccaatt ttcgccagt 
      atcgcgttc tggagcgta ggctctcgg attacatat 
      ttgtgcggt gctttcggg ctgctttgg cccgtcctg 
      actttaacg aaaaagctt taa
    mRNA sequence
    • >TC001773 RA
      aatcgccca aaaaatgta aataaaagt cgcgatttg 
      tgcgttttt gcttgcggt gggccctcg tgttccgct 
      ttgttttgc ttttgcccg aaaatgtga gtctagtga 
      tactttatt tcgcaatca tctgggagc atcaaatta 
      gaaagtagc acttccgct tttactttc caagacgaa 
      aatgtcgca gaaagttga aaaaatgag ttccactgt 
      ttacgtagt ttttctttc ccttttgtt ggatttatt 
      ggcgtttac tatgatcct gccattgtt gccttcgct 
      gctcgagta ttacaagag taacgacag cacggggct 
      gtacgcatg gctctcacg gcgtattca gttcttcca 
      agagctggt cggggcccc cgagaagta caattcggt 
      cctttttgg tggttttct cgggtccat gttcagctt 
      tttgcagtt tgttgtctc ccccatcgt agggggagt 
      ctccgacgt ggtcgggag gaagaaagt catggtcac 
      ttgtctgat tgggatatc aacatcgta cgttctgtg 
      gtgcttgtc gagtaattt agttttgtt tattttggc 
      gcggtttat tggcggaat cagcaaagg caacgtttc 
      cttaagcat ggccataat agccgacgt ctcgacgat 
      gcgaacgcg gggaaaagg catggcttt cgtcggcat 
      tgccttttc cctgggctt catcgttgg ccccctgat 
      aggcgccct gttcgcggt ttgggcgaa gaaaaaagt 
      gggcgattg gttcatagt tcctgcact gttcgcact 
      tttgttgtc gcttgctga cttgatctt tttcatttt 
      ttgtttcaa ggagacgtt accagagga aaaacgtgc 
      caaaagcgt gaaaacgag tctaaacag tgccaaatc 
      gttcatcaa ctggaagga cttgttcca gtttaaggc 
      cgtaacggg cttgaaatc gtccgatat gttcgaatt 
      gaggcgtct gggacgcgt ctatttcgt ctatttgtt 
      catctacag cggtctcga gttcactct gactttttt 
      gacgcatca tatttttaa ttataattc aatgcaaca 
      agggtggat gtttttcag tattggggt cacaatggc 
      gctcatgca agggggata cgtgcgccg aatctcgcc 
      ccaaaaaat cagacccac cgctatttg cggactgtg 
      gattatagt gccttcgtt tgtctgcgt ggggctggc 
      ccaagggcc ccttttgct ctacttcgg actgttttt 
      gttcgcagt ttcgactgc gatggtggt tccgtgcat 
      catgacgat ggcgtcgga gcacggcaa agagagcca 
      gaagggcac agtcatggg cattttcag gtccctggg 
      ggccctagc ccgggcctt gggcccaat tttcgccag 
      tatcgcgtt ctggagcgt aggctctcg gattacata 
      tttgtgcgg tgctttcgg gctgctttg gcccgtcct 
      gactttaac gaaaaagct ttaacagta tttgtatca 
      gtattttat tgtttgtta cc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY