Status
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Gene information
General information
TC001557 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3459388..3463698
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC001557
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_11010

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Fertility
      egg chamber mostlynot present - Number of animals showing the phenotype: 4

      mature egg not present - Number of animals showing the phenotype: 4
      previtellogenic egg chamber not present - Number of animals showing the phenotype: 4

      vitellogenic egg chamber potentially degenerated - Number of animals showing the phenotype: 4

      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC001557 RA
        MSMQLKLLD HIQLNNHVY ANFACDYSE DGKIFLLLE 
        NGVCILTLK GSVENVFPK FSFKKDLIT LSDANICEN 
        LDIDLSSFL NDLTRHNLY EAILDVGLS GDGVNTTPT 
        PPRPVQAMW SPRGVVEKT EGALAILTN LNNIEVYVE 
        VIDENEISS FIRVANFSK DVTDYYRRS WKKVETPNA 
        DNILDELKR RVDLVAPTA FTWSHVILK DRKACCILF 
        VGHRDGAIT AWRFVERKA SEIYESRLQ FLERYNTKL 
        GKITALHWF CRKSDAGGL SLGDVNGHI GALSVSKLS 
        TDKIKFSDE VVFNSESDL KVDKLSIVN YQNITYMIA 
        VKQNFFQIY AINCYGEVF GGKLIQVGN LYITGVLHY 
        EKNVLLVLS LCGQLKQIE LSVVDNEVC VTEKIIQLN 
        IDTNKYRTH GFLFSPNKV FLGILAYPC QIKDISRGK 
        NFVNFFVFG NKSKDPFQT LMNIRAPSI RDYWDCFET 
        LRVNCLQEK VFPWKGIDR NLNYDTLPL LKLKILRWI 
        AKLSEKIYP MISRVEEYH IKAYVLLNY AVDIKLAVQ 
        RMTKLLNIY FSEKKLSLF QMQSLDLLN FFLKEMVVK 
        DVLTKANLG ETFIDEIFN VVKIANELQ YPPMPQCLW 
        CGEKIIGSS CVPFHADSR CAISLMPLN LTPGYKCPF 
        CKCVVSKEI DKEYYPIFC PYCDIPMKR RCFDRKYKK 
        STDDASETT KLEDIITSE CSSDCLQEN IHFSDIEEN 
        TVDYIVLTD SEDEKEDSV KELYSKILK LSVNTPEEF 
        DQSASLSNT KKGQELDQG EKQTEAEDV DDEEEDEQI 
        EEEGEDPAD ENYDGNADE D
      CDS sequence
      • >TC001557 RA
        atgagtatg caattaaaa ctactcgac cacatccag 
        ctaaacaac catgtttat gcaaacttt gcctgtgat 
        tattcggaa gatggaaaa atcttcctc ttgttggag 
        aatggagtg tgtatcttg accctgaag ggttcggtt 
        gaaaacgta ttcccaaag ttttccttc aagaaggat 
        ctgattacg ttatcagat gccaacatt tgtgaaaat 
        ttggacata gatttgtct tcttttttg aatgatttg 
        actcgacat aacttatat gaagcgatt ttggatgtg 
        ggtttatca ggagatggt gtcaatacc acacctacg 
        cccccaaga cccgttcag gcaatgtgg tctccaagg 
        ggggttgtg gagaagacc gaaggtgct ctggctatt 
        ctaacaaac ctcaacaat attgaagta tacgttgaa 
        gtcatcgat gagaacgaa atcagcagt tttattcgg 
        gtcgcgaat ttttccaaa gatgttaca gactattac 
        aggcggagt tggaagaaa gttgaaacg cctaatgca 
        gataatatt ttggatgaa ctgaagagg agggtcgac 
        ctggtcgca cccacggct tttacatgg agccatgtg 
        atacttaag gacagaaaa gcgtgttgt attttattt 
        gtcggtcat agagatggt gctatcaca gcttggcga 
        ttcgtagaa cgcaaagct agtgaaatc tacgaatcg 
        aggttgcaa tttttagaa agatacaac acaaaattg 
        ggtaaaatt acagcattg cattggttt tgccggaaa 
        tcggatgct ggcggttta tcacttggt gatgttaat 
        ggtcacata ggcgcactt tctgtttca aaactgtcc 
        acagataaa atcaagttt agcgatgag gtggttttt 
        aattctgaa agtgactta aaagtggac aaattaagt 
        atcgtcaac taccagaat ataacgtac atgattgca 
        gtaaagcag aattttttt caaatttat gcgattaat 
        tgttacggg gaggttttt ggagggaag ctgatacaa 
        gttggcaac ttgtacatc accggggtc ctacattat 
        gaaaagaac gtcttgctg gttcttagt ttatgcggc 
        caattaaaa caaatagaa ttatcagtg gttgataac 
        gaagtttgt gttacggaa aaaataatt cagctaaac 
        atcgacacc aataaatac cgaactcat ggattccta 
        ttctcaccg aataaagtt ttcctcggt atacttgca 
        tatccttgc caaataaaa gacataagt cggggaaaa 
        aacttcgtc aactttttt gtattcggt aacaaatcc 
        aaagacccc ttccagact ttaatgaat attcgcgcg 
        cccagtatt agggattat tgggactgt tttgaaacg 
        ttaagagta aattgtttg caagaaaaa gtctttcct 
        tggaaaggc atcgatcgt aatttaaat tacgacact 
        ttaccgtta ttaaagtta aaaatactg cgctggatt 
        gccaaatta tcggaaaaa atatatcca atgatttca 
        cgagttgaa gaatatcat atcaaagcg tacgttttg 
        ttgaattac gctgtcgat attaaatta gcagtgcaa 
        cgaatgaca aaattgttg aatatttat ttctccgaa 
        aaaaaactc tcactgttt caaatgcaa agcttggac 
        ttgcttaat ttctttttg aaagaaatg gtggtcaaa 
        gacgtacta actaaggca aatttgggc gaaacattc 
        attgatgaa atcttcaac gttgttaag atagccaac 
        gaactgcaa tacccacca atgcctcag tgtctatgg 
        tgtggagaa aagatcata ggttcgtct tgtgtacct 
        tttcacgct gatagcaga tgtgccatc tccctaatg 
        cccctaaac ctcacccct ggctacaag tgtccgttt 
        tgtaaatgt gtggtaagc aaggaaatc gacaaggag 
        tactacccc attttttgc ccctactgt gatatcccc 
        atgaaacgc cgctgtttc gatcgaaag tacaaaaaa 
        agcacagat gatgcaagc gaaacaacc aaacttgaa 
        gacatcatc acttcagaa tgtagcagc gattgcctc 
        caagaaaac atccatttc agcgacatt gaagaaaac 
        accgttgat tatatcgtg ctaactgac agcgaagac 
        gagaaagaa gactcagtt aaggaactc tattcgaaa 
        attttgaaa ctttcagtt aatacacct gaagagttc 
        gaccaatcc gcctcattg tctaacaca aagaaaggg 
        caggagttg gatcaaggt gagaaacaa accgaggct 
        gaagacgtg gacgacgag gaagaggac gagcaaatt 
        gaagaagag ggtgaagac ccagctgat gaaaattac 
        gatggcaat gcagatgag gattga
      mRNA sequence
      • >TC001557 RA
        ttcgaaaca acaagtgtt tattttaac ctcacttgt 
        agtgttatg tgtgccact gtgtaaaat cagtaaaaa 
        ccaaaacca aatattctt aactcttgc aaaacgctc 
        actgataac gttcttaag cataatgag ttattaatt 
        ccctttacg caaccatga gtatgcaat taaaactac 
        tcgaccaca tccagctaa acaaccatg tttatgcaa 
        actttgcct gtgattatt cggaagatg gaaaaatct 
        tcctcttgt tggagaatg gagtgtgta tcttgaccc 
        tgaagggtt cggttgaaa acgtattcc caaagtttt 
        ccttcaaga aggatctga ttacgttat cagatgcca 
        acatttgtg aaaatttgg acatagatt tgtcttctt 
        ttttgaatg atttgactc gacataact tatatgaag 
        cgattttgg atgtgggtt tatcaggag atggtgtca 
        ataccacac ctacgcccc caagacccg ttcaggcaa 
        tgtggtctc caagggggg ttgtggaga agaccgaag 
        gtgctctgg ctattctaa caaacctca acaatattg 
        aagtatacg ttgaagtca tcgatgaga acgaaatca 
        gcagtttta ttcgggtcg cgaattttt ccaaagatg 
        ttacagact attacaggc ggagttgga agaaagttg 
        aaacgccta atgcagata atattttgg atgaactga 
        agaggaggg tcgacctgg tcgcaccca cggctttta 
        catggagcc atgtgatac ttaaggaca gaaaagcgt 
        gttgtattt tatttgtcg gtcatagag atggtgcta 
        tcacagctt ggcgattcg tagaacgca aagctagtg 
        aaatctacg aatcgaggt tgcaatttt tagaaagat 
        acaacacaa aattgggta aaattacag cattgcatt 
        ggttttgcc ggaaatcgg atgctggcg gtttatcac 
        ttggtgatg ttaatggtc acataggcg cactttctg 
        tttcaaaac tgtccacag ataaaatca agtttagcg 
        atgaggtgg tttttaatt ctgaaagtg acttaaaag 
        tggacaaat taagtatcg tcaactacc agaatataa 
        cgtacatga ttgcagtaa agcagaatt tttttcaaa 
        tttatgcga ttaattgtt acggggagg tttttggag 
        ggaagctga tacaagttg gcaacttgt acatcaccg 
        gggtcctac attatgaaa agaacgtct tgctggttc 
        ttagtttat gcggccaat taaaacaaa tagaattat 
        cagtggttg ataacgaag tttgtgtta cggaaaaaa 
        taattcagc taaacatcg acaccaata aataccgaa 
        ctcatggat tcctattct caccgaata aagttttcc 
        tcggtatac ttgcatatc cttgccaaa taaaagaca 
        taagtcggg gaaaaaact tcgtcaact tttttgtat 
        tcggtaaca aatccaaag accccttcc agactttaa 
        tgaatattc gcgcgccca gtattaggg attattggg 
        actgttttg aaacgttaa gagtaaatt gtttgcaag 
        aaaaagtct ttccttgga aaggcatcg atcgtaatt 
        taaattacg acactttac cgttattaa agttaaaaa 
        tactgcgct ggattgcca aattatcgg aaaaaatat 
        atccaatga tttcacgag ttgaagaat atcatatca 
        aagcgtacg ttttgttga attacgctg tcgatatta 
        aattagcag tgcaacgaa tgacaaaat tgttgaata 
        tttatttct ccgaaaaaa aactctcac tgtttcaaa 
        tgcaaagct tggacttgc ttaatttct ttttgaaag 
        aaatggtgg tcaaagacg tactaacta aggcaaatt 
        tgggcgaaa cattcattg atgaaatct tcaacgttg 
        ttaagatag ccaacgaac tgcaatacc caccaatgc 
        ctcagtgtc tatggtgtg gagaaaaga tcataggtt 
        cgtcttgtg taccttttc acgctgata gcagatgtg 
        ccatctccc taatgcccc taaacctca cccctggct 
        acaagtgtc cgttttgta aatgtgtgg taagcaagg 
        aaatcgaca aggagtact accccattt tttgcccct 
        actgtgata tccccatga aacgccgct gtttcgatc 
        gaaagtaca aaaaaagca cagatgatg caagcgaaa 
        caaccaaac ttgaagaca tcatcactt cagaatgta 
        gcagcgatt gcctccaag aaaacatcc atttcagcg 
        acattgaag aaaacaccg ttgattata tcgtgctaa 
        ctgacagcg aagacgaga aagaagact cagttaagg 
        aactctatt cgaaaattt tgaaacttt cagttaata 
        cacctgaag agttcgacc aatccgcct cattgtcta 
        acacaaaga aagggcagg agttggatc aaggtgaga 
        aacaaaccg aggctgaag acgtggacg acgaggaag 
        aggacgagc aaattgaag aagagggtg aagacccag 
        ctgatgaaa attacgatg gcaatgcag atgaggatt 
        gagattttt gtaattgaa aatattggt aaggtaata 
        aattttaac gatggttaa aactcgagt aacttctat 
        tgtacaaaa atacaaact ttgactagt tacaaaaca 
        gcctttttg actaggtcc cgttgattt taacaaatc 
        actggtaaa tattgtaca cactgtggt cattatgtc 
        cacgattat tctcccagt acctaaaat tactccttc 
        cctcgctgt ttgtctcgc attcaaacc tgtagcccg 
        gccaatagg aattgtagt aattgccgg caatgtaag 
        gctgtgtgt aattactcc cataattac aattattgt 
        tgtacctgt cttggtagc cacaagtgg tgttatttt 
        caggcataa acaccctcc gataattta aatgttttc 
        cgccgtttc ccatcaaac tcccggtta gtaaattgg 
        cggtagttt ggcttaaat cccgactca aaattttaa 
        taaacctgg tcaattcca tagggcggc cttcttgca 
        acgtttgag acattgcta caatgtagt tccttgcca 
        gacgtagag tttcaattt ttgagagtt tttgtggcg 
        ccgaactca aatctgagt gtgtttgtt gaatgggaa 
        tgcggctga aaaataata catcttaat taaaaaatt 
        gtaattgat tgtttgtca tttgataaa acctacttt 
        cggagtctg tctaaagcg gcgtttagt ccgtgcatg 
        cggtttctc tccctcgcg tttgcttta caccgtctc 
        agcttcact ttattgtgc actctaatt ttcacgtct 
        tttctttca ccgtttttt acgaccggt tccattgca 
        ccaaatagt cgtaaataa atcgaaaat tcttta
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY