Status
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Gene information
General information
TC001557 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3459388..3463698
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC001557
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes

      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_11010

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection

      Fertility
      egg chamber mostlynot present - Number of animals showing the phenotype: 4

      mature egg not present - Number of animals showing the phenotype: 4
      previtellogenic egg chamber not present - Number of animals showing the phenotype: 4

      vitellogenic egg chamber potentially degenerated - Number of animals showing the phenotype: 4

      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC001557 RA
        MSMQLKLLD HIQLNNHVY ANFACDYSE DGKIFLLLE 
NGVCILTLK GSVENVFPK FSFKKDLIT LSDANICEN 
LDIDLSSFL NDLTRHNLY EAILDVGLS GDGVNTTPT 
PPRPVQAMW SPRGVVEKT EGALAILTN LNNIEVYVE 
VIDENEISS FIRVANFSK DVTDYYRRS WKKVETPNA 
DNILDELKR RVDLVAPTA FTWSHVILK DRKACCILF 
VGHRDGAIT AWRFVERKA SEIYESRLQ FLERYNTKL 
GKITALHWF CRKSDAGGL SLGDVNGHI GALSVSKLS 
TDKIKFSDE VVFNSESDL KVDKLSIVN YQNITYMIA 
VKQNFFQIY AINCYGEVF GGKLIQVGN LYITGVLHY 
EKNVLLVLS LCGQLKQIE LSVVDNEVC VTEKIIQLN 
IDTNKYRTH GFLFSPNKV FLGILAYPC QIKDISRGK 
NFVNFFVFG NKSKDPFQT LMNIRAPSI RDYWDCFET 
LRVNCLQEK VFPWKGIDR NLNYDTLPL LKLKILRWI 
AKLSEKIYP MISRVEEYH IKAYVLLNY AVDIKLAVQ 
RMTKLLNIY FSEKKLSLF QMQSLDLLN FFLKEMVVK 
DVLTKANLG ETFIDEIFN VVKIANELQ YPPMPQCLW 
CGEKIIGSS CVPFHADSR CAISLMPLN LTPGYKCPF 
CKCVVSKEI DKEYYPIFC PYCDIPMKR RCFDRKYKK 
STDDASETT KLEDIITSE CSSDCLQEN IHFSDIEEN 
TVDYIVLTD SEDEKEDSV KELYSKILK LSVNTPEEF 
DQSASLSNT KKGQELDQG EKQTEAEDV DDEEEDEQI 
EEEGEDPAD ENYDGNADE D
      CDS sequence
      • >TC001557 RA
        atgagtatg caattaaaa ctactcgac cacatccag 
ctaaacaac catgtttat gcaaacttt gcctgtgat 
tattcggaa gatggaaaa atcttcctc ttgttggag 
aatggagtg tgtatcttg accctgaag ggttcggtt 
gaaaacgta ttcccaaag ttttccttc aagaaggat 
ctgattacg ttatcagat gccaacatt tgtgaaaat 
ttggacata gatttgtct tcttttttg aatgatttg 
actcgacat aacttatat gaagcgatt ttggatgtg 
ggtttatca ggagatggt gtcaatacc acacctacg 
cccccaaga cccgttcag gcaatgtgg tctccaagg 
ggggttgtg gagaagacc gaaggtgct ctggctatt 
ctaacaaac ctcaacaat attgaagta tacgttgaa 
gtcatcgat gagaacgaa atcagcagt tttattcgg 
gtcgcgaat ttttccaaa gatgttaca gactattac 
aggcggagt tggaagaaa gttgaaacg cctaatgca 
gataatatt ttggatgaa ctgaagagg agggtcgac 
ctggtcgca cccacggct tttacatgg agccatgtg 
atacttaag gacagaaaa gcgtgttgt attttattt 
gtcggtcat agagatggt gctatcaca gcttggcga 
ttcgtagaa cgcaaagct agtgaaatc tacgaatcg 
aggttgcaa tttttagaa agatacaac acaaaattg 
ggtaaaatt acagcattg cattggttt tgccggaaa 
tcggatgct ggcggttta tcacttggt gatgttaat 
ggtcacata ggcgcactt tctgtttca aaactgtcc 
acagataaa atcaagttt agcgatgag gtggttttt 
aattctgaa agtgactta aaagtggac aaattaagt 
atcgtcaac taccagaat ataacgtac atgattgca 
gtaaagcag aattttttt caaatttat gcgattaat 
tgttacggg gaggttttt ggagggaag ctgatacaa 
gttggcaac ttgtacatc accggggtc ctacattat 
gaaaagaac gtcttgctg gttcttagt ttatgcggc 
caattaaaa caaatagaa ttatcagtg gttgataac 
gaagtttgt gttacggaa aaaataatt cagctaaac 
atcgacacc aataaatac cgaactcat ggattccta 
ttctcaccg aataaagtt ttcctcggt atacttgca 
tatccttgc caaataaaa gacataagt cggggaaaa 
aacttcgtc aactttttt gtattcggt aacaaatcc 
aaagacccc ttccagact ttaatgaat attcgcgcg 
cccagtatt agggattat tgggactgt tttgaaacg 
ttaagagta aattgtttg caagaaaaa gtctttcct 
tggaaaggc atcgatcgt aatttaaat tacgacact 
ttaccgtta ttaaagtta aaaatactg cgctggatt 
gccaaatta tcggaaaaa atatatcca atgatttca 
cgagttgaa gaatatcat atcaaagcg tacgttttg 
ttgaattac gctgtcgat attaaatta gcagtgcaa 
cgaatgaca aaattgttg aatatttat ttctccgaa 
aaaaaactc tcactgttt caaatgcaa agcttggac 
ttgcttaat ttctttttg aaagaaatg gtggtcaaa 
gacgtacta actaaggca aatttgggc gaaacattc 
attgatgaa atcttcaac gttgttaag atagccaac 
gaactgcaa tacccacca atgcctcag tgtctatgg 
tgtggagaa aagatcata ggttcgtct tgtgtacct 
tttcacgct gatagcaga tgtgccatc tccctaatg 
cccctaaac ctcacccct ggctacaag tgtccgttt 
tgtaaatgt gtggtaagc aaggaaatc gacaaggag 
tactacccc attttttgc ccctactgt gatatcccc 
atgaaacgc cgctgtttc gatcgaaag tacaaaaaa 
agcacagat gatgcaagc gaaacaacc aaacttgaa 
gacatcatc acttcagaa tgtagcagc gattgcctc 
caagaaaac atccatttc agcgacatt gaagaaaac 
accgttgat tatatcgtg ctaactgac agcgaagac 
gagaaagaa gactcagtt aaggaactc tattcgaaa 
attttgaaa ctttcagtt aatacacct gaagagttc 
gaccaatcc gcctcattg tctaacaca aagaaaggg 
caggagttg gatcaaggt gagaaacaa accgaggct 
gaagacgtg gacgacgag gaagaggac gagcaaatt 
gaagaagag ggtgaagac ccagctgat gaaaattac 
gatggcaat gcagatgag gattga
      mRNA sequence
      • >TC001557 RA
        ttcgaaaca acaagtgtt tattttaac ctcacttgt 
agtgttatg tgtgccact gtgtaaaat cagtaaaaa 
ccaaaacca aatattctt aactcttgc aaaacgctc 
actgataac gttcttaag cataatgag ttattaatt 
ccctttacg caaccatga gtatgcaat taaaactac 
tcgaccaca tccagctaa acaaccatg tttatgcaa 
actttgcct gtgattatt cggaagatg gaaaaatct 
tcctcttgt tggagaatg gagtgtgta tcttgaccc 
tgaagggtt cggttgaaa acgtattcc caaagtttt 
ccttcaaga aggatctga ttacgttat cagatgcca 
acatttgtg aaaatttgg acatagatt tgtcttctt 
ttttgaatg atttgactc gacataact tatatgaag 
cgattttgg atgtgggtt tatcaggag atggtgtca 
ataccacac ctacgcccc caagacccg ttcaggcaa 
tgtggtctc caagggggg ttgtggaga agaccgaag 
gtgctctgg ctattctaa caaacctca acaatattg 
aagtatacg ttgaagtca tcgatgaga acgaaatca 
gcagtttta ttcgggtcg cgaattttt ccaaagatg 
ttacagact attacaggc ggagttgga agaaagttg 
aaacgccta atgcagata atattttgg atgaactga 
agaggaggg tcgacctgg tcgcaccca cggctttta 
catggagcc atgtgatac ttaaggaca gaaaagcgt 
gttgtattt tatttgtcg gtcatagag atggtgcta 
tcacagctt ggcgattcg tagaacgca aagctagtg 
aaatctacg aatcgaggt tgcaatttt tagaaagat 
acaacacaa aattgggta aaattacag cattgcatt 
ggttttgcc ggaaatcgg atgctggcg gtttatcac 
ttggtgatg ttaatggtc acataggcg cactttctg 
tttcaaaac tgtccacag ataaaatca agtttagcg 
atgaggtgg tttttaatt ctgaaagtg acttaaaag 
tggacaaat taagtatcg tcaactacc agaatataa 
cgtacatga ttgcagtaa agcagaatt tttttcaaa 
tttatgcga ttaattgtt acggggagg tttttggag 
ggaagctga tacaagttg gcaacttgt acatcaccg 
gggtcctac attatgaaa agaacgtct tgctggttc 
ttagtttat gcggccaat taaaacaaa tagaattat 
cagtggttg ataacgaag tttgtgtta cggaaaaaa 
taattcagc taaacatcg acaccaata aataccgaa 
ctcatggat tcctattct caccgaata aagttttcc 
tcggtatac ttgcatatc cttgccaaa taaaagaca 
taagtcggg gaaaaaact tcgtcaact tttttgtat 
tcggtaaca aatccaaag accccttcc agactttaa 
tgaatattc gcgcgccca gtattaggg attattggg 
actgttttg aaacgttaa gagtaaatt gtttgcaag 
aaaaagtct ttccttgga aaggcatcg atcgtaatt 
taaattacg acactttac cgttattaa agttaaaaa 
tactgcgct ggattgcca aattatcgg aaaaaatat 
atccaatga tttcacgag ttgaagaat atcatatca 
aagcgtacg ttttgttga attacgctg tcgatatta 
aattagcag tgcaacgaa tgacaaaat tgttgaata 
tttatttct ccgaaaaaa aactctcac tgtttcaaa 
tgcaaagct tggacttgc ttaatttct ttttgaaag 
aaatggtgg tcaaagacg tactaacta aggcaaatt 
tgggcgaaa cattcattg atgaaatct tcaacgttg 
ttaagatag ccaacgaac tgcaatacc caccaatgc 
ctcagtgtc tatggtgtg gagaaaaga tcataggtt 
cgtcttgtg taccttttc acgctgata gcagatgtg 
ccatctccc taatgcccc taaacctca cccctggct 
acaagtgtc cgttttgta aatgtgtgg taagcaagg 
aaatcgaca aggagtact accccattt tttgcccct 
actgtgata tccccatga aacgccgct gtttcgatc 
gaaagtaca aaaaaagca cagatgatg caagcgaaa 
caaccaaac ttgaagaca tcatcactt cagaatgta 
gcagcgatt gcctccaag aaaacatcc atttcagcg 
acattgaag aaaacaccg ttgattata tcgtgctaa 
ctgacagcg aagacgaga aagaagact cagttaagg 
aactctatt cgaaaattt tgaaacttt cagttaata 
cacctgaag agttcgacc aatccgcct cattgtcta 
acacaaaga aagggcagg agttggatc aaggtgaga 
aacaaaccg aggctgaag acgtggacg acgaggaag 
aggacgagc aaattgaag aagagggtg aagacccag 
ctgatgaaa attacgatg gcaatgcag atgaggatt 
gagattttt gtaattgaa aatattggt aaggtaata 
aattttaac gatggttaa aactcgagt aacttctat 
tgtacaaaa atacaaact ttgactagt tacaaaaca 
gcctttttg actaggtcc cgttgattt taacaaatc 
actggtaaa tattgtaca cactgtggt cattatgtc 
cacgattat tctcccagt acctaaaat tactccttc 
cctcgctgt ttgtctcgc attcaaacc tgtagcccg 
gccaatagg aattgtagt aattgccgg caatgtaag 
gctgtgtgt aattactcc cataattac aattattgt 
tgtacctgt cttggtagc cacaagtgg tgttatttt 
caggcataa acaccctcc gataattta aatgttttc 
cgccgtttc ccatcaaac tcccggtta gtaaattgg 
cggtagttt ggcttaaat cccgactca aaattttaa 
taaacctgg tcaattcca tagggcggc cttcttgca 
acgtttgag acattgcta caatgtagt tccttgcca 
gacgtagag tttcaattt ttgagagtt tttgtggcg 
ccgaactca aatctgagt gtgtttgtt gaatgggaa 
tgcggctga aaaataata catcttaat taaaaaatt 
gtaattgat tgtttgtca tttgataaa acctacttt 
cggagtctg tctaaagcg gcgtttagt ccgtgcatg 
cggtttctc tccctcgcg tttgcttta caccgtctc 
agcttcact ttattgtgc actctaatt ttcacgtct 
tttctttca ccgtttttt acgaccggt tccattgca 
ccaaatagt cgtaaataa atcgaaaat tcttta
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY