Status
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Gene information
General information
TC015348 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 119074..122984
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015348
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_11005

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015348 RA
      MEAMQYELA RNMTLLFFF ERLLDKGEP RTLHDLSCQ 
      FGSKGFTKE MRQIAGGSQ SGLKKFLAQ YPALFAIEG 
      EYVTINMFQ NLGSKDSPG GKDYTTQAV EYFSEKLSQ 
      YGEGTEVPI RSLLGHRSQ ASPEVRHVS GQHFHEFRE 
      FLLKHPDTF TIDDEKETV ILTNYTAVR SHCPPELHF 
      QPDVKIDPE TTQTLLDFL AQCIEVKGP ILVEQLFQI 
      VSCNLPEAM WTNLFNTPA HLTSFLRLF SDSFHIQAN 
      LVTLLQPPK VSQKHLNLD LEAKNAKNL NNCQDSSSE 
      IIERAPPVT SPKSISDRL KQPKLQQKQ LSAEAKSLS 
      PEPPKQKSP PSNQSLKQR INNLVLKTL QENTGRERQ 
      SFLNNQSHN ESWKVKLFQ NTRVICSVR ECQMVIDDI 
      MSKVKSQMS NDWPFDDDQ VVVGLDCEG INLGVKGQL 
      TLLQIATMS GFSYVFDLI TCPGMIDSG LKKLLESSQ 
      IVKIVHDCR NDSVNLFNQ FNITLNTIF DTQAAHAVL 
      TFQETGRPV YKAKSVALN ALCEHYGAP INPMKDQLK 
      NIYRRDQKY WSRRPLTRE MILYASADV LSLVNEKIY 
      YPMSKAIKM ENRQLFLEL CHEQIFMHI DPESVKGRK 
      RQRKTETEV SELRQKLSQ ATKNVVLSN REVRLLRYI 
      ELTEEEKEK LKSSAKVAK KLEKLESLG QDNRDDDED 
      DEDQDNDPD YPSLESDMT SPRNSEPTS LTESMQLVD 
      SILNDSKIE KLDKLDRLE TILSTAAFL PTGGKCQCQ 
      CHKNGYNGE RVISPTSSF KGEDREVEV KSNHSNIGT 
      QTLSTGDIV VTKIFFNEN TE
    CDS sequence
    • >TC015348 RA
      atggaggca atgcaatat gagttggct cgaaacatg 
      acgttgttg ttctttttc gagcgccta ttagataag 
      ggcgaacca agaaccctt cacgatttg agttgtcaa 
      tttggctca aagggcttc accaaggaa atgcgccaa 
      atcgccgga gggtcacaa agtggcctt aagaagttt 
      ctagcacaa tatccggcc ctttttgcg attgagggt 
      gaatatgtt acaataaac atgttccag aatctgggg 
      agtaaagac agccctggg ggcaaagac tacacaact 
      caagccgtt gaatatttc tccgaaaag ttgtcccaa 
      tatggtgaa ggcaccgaa gtcccaatt cgcagtctt 
      ttggggcac cgttcccaa gccagtcca gaggtacgt 
      cacgtttcg ggccaacac ttccacgaa ttcagggaa 
      tttcttctt aaacatccc gacacattt acaattgac 
      gacgaaaaa gaaaccgta attttaaca aattataca 
      gccgttcgc tcgcattgc cccccagag ctgcacttc 
      caacccgac gttaaaatc gaccctgaa accacacaa 
      acactcctc gattttctt gctcaatgt atcgaagtt 
      aaagggccg attttagtc gaacagttg ttccaaatc 
      gtcagttgt aacctacca gaggctatg tggacaaat 
      ttatttaac acaccggca cacttgacc agttttttg 
      cgtcttttt tcggacagt tttcacatc caagccaac 
      ctagtgaca ttgctgcag ccccctaaa gtcagtcag 
      aagcattta aatttggat ttggaggcg aaaaatgcc 
      aaaaattta aacaactgt caagatagt tcgagtgag 
      attattgaa agggcgcca ccggttact agtcccaag 
      tcaattagt gataggttg aaacagccc aaattgcaa 
      caaaaacaa ctatctgct gaagcgaaa agtttatca 
      cccgaaccg cctaaacaa aaatcaccg ccttctaat 
      caaagtctc aaacagagg attaataat ctcgtcttg 
      aaaacgttg caagaaaat acggggcgt gaaagacag 
      tcgtttctc aataatcaa agtcataac gagtcatgg 
      aaagtcaag ttgtttcaa aatacgcgc gttatatgc 
      agtgtcagg gaatgtcaa atggtgatt gatgacatt 
      atgtcaaag gttaagtct caaatgtct aatgattgg 
      ccgtttgat gacgatcaa gtcgtcgtt ggattggac 
      tgtgaaggg attaattta ggggttaag ggacaatta 
      actttgctt caaattgca acaatgagt ggattttcg 
      tacgttttt gatttgata acatgtccc ggaatgatt 
      gattccggt ttaaaaaaa ctactcgaa agttcacag 
      attgttaag attgtgcac gattgtcgt aacgattcc 
      gttaatctt ttcaatcag tttaacatt acccttaat 
      acaattttc gatactcaa gcagcacat gctgttcta 
      acgtttcaa gaaactgga cgtcctgtt tataaggcg 
      aaaagtgta gcgttaaac gccctctgt gaacattat 
      ggtgcacca attaatcca atgaaagat caactcaag 
      aatatttat cgacgtgac cagaaatat tggtcaaga 
      aggccatta acacgtgaa atgattttg tatgcgtct 
      gctgatgtt ttaagtttg gttaatgag aaaatttat 
      tatcccatg tcgaaggcg attaagatg gagaatcgg 
      cagttgttt ttggagttg tgccatgaa cagattttc 
      atgcacatc gaccctgaa tctgtcaag ggccgcaaa 
      cgtcaacgc aaaaccgaa accgaagtg tcagaatta 
      cgtcaaaaa ctgtcacaa gcgacaaaa aatgtagtt 
      ttaagtaat cgtgaagtg cgacttttg cgttacatt 
      gaattaacc gaagaagag aaagagaaa ttaaaatcg 
      tcggcaaaa gttgcaaaa aaactcgaa aaattggaa 
      agtttaggt caggataat agagatgat gatgaagat 
      gatgaggat caggataat gatcctgat tatcctagt 
      ttagagagt gatatgacg tcaccacgt aattccgaa 
      ccaacaagt cttacagaa tcaatgcaa ttagttgat 
      tcgatttta aacgatagt aagattgaa aaattggat 
      aaattggat cgtttagag acgatttta tcgacggct 
      gcgttttta ccgactggt ggtaaatgt caatgtcag 
      tgtcataaa aatggttat aatggcgaa agggttatt 
      tcgccaact agtagtttc aaaggtgag gatagagaa 
      gtagaagtt aagtcgaat cattcaaat attggtact 
      caaacgcta agtactgga gatatagtc gtgacgaag 
      atttttttt aatgaaaat actgaatag
    mRNA sequence
    • >TC015348 RA
      acgaaataa acaacccat tctggtggt tttagcgtt 
      ttgtggatg ttgaataat tatattatt gctgaaaga 
      tgaaattat tgagcaaaa gttcaatta acgttgaaa 
      aatcaatta atttgttat ttgttggtg tttgcaatg 
      acgtcacag cccacctga cctgtcaaa agaattcaa 
      aatggcgtc gtcggccaa cttgacgtt gtgaacgtc 
      gcaaaaaat cactttttc ggttaattt tttaattta 
      attttttca ttaaaacgt ctccgatgt tacaaacac 
      acctgcaag ttcaagtgt taagtgctt aaacctcaa 
      acatgttgt attaaaaaa gaaaaaaca gagtgaacc 
      atggaggca atgcaatat gagttggct cgaaacatg 
      acgttgttg ttctttttc gagcgccta ttagataag 
      ggcgaacca agaaccctt cacgatttg agttgtcaa 
      tttggctca aagggcttc accaaggaa atgcgccaa 
      atcgccgga gggtcacaa agtggcctt aagaagttt 
      ctagcacaa tatccggcc ctttttgcg attgagggt 
      gaatatgtt acaataaac atgttccag aatctgggg 
      agtaaagac agccctggg ggcaaagac tacacaact 
      caagccgtt gaatatttc tccgaaaag ttgtcccaa 
      tatggtgaa ggcaccgaa gtcccaatt cgcagtctt 
      ttggggcac cgttcccaa gccagtcca gaggtacgt 
      cacgtttcg ggccaacac ttccacgaa ttcagggaa 
      tttcttctt aaacatccc gacacattt acaattgac 
      gacgaaaaa gaaaccgta attttaaca aattataca 
      gccgttcgc tcgcattgc cccccagag ctgcacttc 
      caacccgac gttaaaatc gaccctgaa accacacaa 
      acactcctc gattttctt gctcaatgt atcgaagtt 
      aaagggccg attttagtc gaacagttg ttccaaatc 
      gtcagttgt aacctacca gaggctatg tggacaaat 
      ttatttaac acaccggca cacttgacc agttttttg 
      cgtcttttt tcggacagt tttcacatc caagccaac 
      ctagtgaca ttgctgcag ccccctaaa gtcagtcag 
      aagcattta aatttggat ttggaggcg aaaaatgcc 
      aaaaattta aacaactgt caagatagt tcgagtgag 
      attattgaa agggcgcca ccggttact agtcccaag 
      tcaattagt gataggttg aaacagccc aaattgcaa 
      caaaaacaa ctatctgct gaagcgaaa agtttatca 
      cccgaaccg cctaaacaa aaatcaccg ccttctaat 
      caaagtctc aaacagagg attaataat ctcgtcttg 
      aaaacgttg caagaaaat acggggcgt gaaagacag 
      tcgtttctc aataatcaa agtcataac gagtcatgg 
      aaagtcaag ttgtttcaa aatacgcgc gttatatgc 
      agtgtcagg gaatgtcaa atggtgatt gatgacatt 
      atgtcaaag gttaagtct caaatgtct aatgattgg 
      ccgtttgat gacgatcaa gtcgtcgtt ggattggac 
      tgtgaaggg attaattta ggggttaag ggacaatta 
      actttgctt caaattgca acaatgagt ggattttcg 
      tacgttttt gatttgata acatgtccc ggaatgatt 
      gattccggt ttaaaaaaa ctactcgaa agttcacag 
      attgttaag attgtgcac gattgtcgt aacgattcc 
      gttaatctt ttcaatcag tttaacatt acccttaat 
      acaattttc gatactcaa gcagcacat gctgttcta 
      acgtttcaa gaaactgga cgtcctgtt tataaggcg 
      aaaagtgta gcgttaaac gccctctgt gaacattat 
      ggtgcacca attaatcca atgaaagat caactcaag 
      aatatttat cgacgtgac cagaaatat tggtcaaga 
      aggccatta acacgtgaa atgattttg tatgcgtct 
      gctgatgtt ttaagtttg gttaatgag aaaatttat 
      tatcccatg tcgaaggcg attaagatg gagaatcgg 
      cagttgttt ttggagttg tgccatgaa cagattttc 
      atgcacatc gaccctgaa tctgtcaag ggccgcaaa 
      cgtcaacgc aaaaccgaa accgaagtg tcagaatta 
      cgtcaaaaa ctgtcacaa gcgacaaaa aatgtagtt 
      ttaagtaat cgtgaagtg cgacttttg cgttacatt 
      gaattaacc gaagaagag aaagagaaa ttaaaatcg 
      tcggcaaaa gttgcaaaa aaactcgaa aaattggaa 
      agtttaggt caggataat agagatgat gatgaagat 
      gatgaggat caggataat gatcctgat tatcctagt 
      ttagagagt gatatgacg tcaccacgt aattccgaa 
      ccaacaagt cttacagaa tcaatgcaa ttagttgat 
      tcgatttta aacgatagt aagattgaa aaattggat 
      aaattggat cgtttagag acgatttta tcgacggct 
      gcgttttta ccgactggt ggtaaatgt caatgtcag 
      tgtcataaa aatggttat aatggcgaa agggttatt 
      tcgccaact agtagtttc aaaggtgag gatagagaa 
      gtagaagtt aagtcgaat cattcaaat attggtact 
      caaacgcta agtactgga gatatagtc gtgacgaag 
      atttttttt aatgaaaat actgaatag ctcaatggt 
      cacgtggcc ttattgtat agtcggttg tatataatt 
      attaattat ttgatcgag ctgatttta cttaatgtt 
      atcgagcca tatttttaa tagtgttgt tatggtgat 
      ttaatttat tgtttatga gacgacatc agtctttgt 
      ttcttaaat attttcgtt aagacaact aagacttga 
      ggaataacg gtattaaca ttgttaagc aatatttat 
      gtgttatta acatgtatt atttttgct ggtagagga 
      tttttatta tactgtaat gatgcaaaa agaaaataa 
      atacagtac taaaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY