Status
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Gene information
General information
TC013173 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12729028..12733355
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC013173
      iBeetle screen iB_11004

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC013173 RA
        MFRQANSVR ALFVLCLFK IIACDGSFH LFYQIPASE 
        YDGCENLSR FFQTIQTSS PVNALKAII APLACSTCP 
        QESHLLHQK LIELASHGS LNINFVIAN LKKMKNLNR 
        YDVLITTLM AINEFTTVD PSLKLEISN EIFYLLKRR 
        YLKINSYSK SRQGFCQYD AAKPQVGGI FSRPKQTPI 
        LIQTPIRND YLRAPVGRI SQPFVSQIP VLIQTPIKY 
        YPKAAPSYV PPVVQHVMG KALPTNQAN GCACQTRVI 
        EMLLDRLIA LERQRKQPT TDQSCRNTR IQPNKTSTP 
        SDSLRFRSN PKTSGLNVP IVPRNNLDV LQDLLKDFP 
        LPKYPKEKM MYQKFKALL QKPQVQNLV KNLDLSKYK 
        PNEILYIII TTVLHTLKT EEIQEVFAF FETYREAAR 
        TGFNFGNLL DLLPPPKSS KERNYHNIT KHLLLSRAL 
        YNLNLGPNF RQAPNLADK LIILLQELL KTNTIPGEV 
        REALQYYLP VFLGKRANI HFENLIPLL PKPVNDVER 
        KQLDVLKNL LASNELYVL MKNYNLNNL TPAQLLQIV 
        LHHLINQHG DYSDAALYY RNKLAINKY GFNYYDLLN 
        LIPVTQENR AFVQLLSNY FHSPEFEAI LALPSLKTL 
        SNVDKLLLI LETIQKKST DANVLKAAA SVLDDVRLQ 
        VFYQNIQQK VIGLIPAPK SNIEKQQYE TVKSFLLSK 
        RAFPMLGGI DLSVFPDSK HLLEFVFKT VTESNLEQN 
        IRDAFEYYL DIAHKLKPL EFVTKKEIK KKFELTEIF 
        TDTLDLKTL TVPQKRAFK NFFKYISEV KPDAMSKFD 
        SWDQAKTRG EFMKLLFKY FVESPTVLP EIKESIKIL 
        DPLVKMDGK GALPP
      CDS sequence
      • >TC013173 RA
        atgtttcgt caggctaac agtgtgagg gcacttttc 
        gtgctttgc ttgttcaaa atcattgca tgcgatggc 
        tctttccac cttttctat caaatccct gcaagtgaa 
        tatgacggc tgcgaaaac ctgtcaaga ttcttccaa 
        acgattcaa acatcgtcc ccagtcaat gctctaaaa 
        gcgatcatt gcaccactg gcctgcagc acttgtccg 
        caagaatct cacctccta caccaaaaa ttaatcgaa 
        ctggcatca cacggctcg ttaaatatc aactttgtt 
        atcgcaaat ctcaaaaaa atgaaaaac ttgaacaga 
        tacgatgtt ttaattacc actttgatg gcaattaac 
        gagtttaca acggttgac cccagcttg aagcttgaa 
        atcagcaac gagatcttt tatttgctc aagaggcgg 
        tacttgaag atcaatagc tactccaaa tcacgccaa 
        gggttttgt cagtacgat gctgcgaag ccccaagtc 
        ggcggaatt ttctcaagg cctaagcaa acccccata 
        ttaattcaa acgcccatc aggaatgat tatttaagg 
        gctccagtt ggtagaatc tcacagcca tttgtgtcc 
        caaattcct gttttaatt caaactcct atcaagtac 
        tatccaaag gctgctcct agttatgtt cctccagtg 
        gttcagcac gtcatgggc aaagcttta ccgaccaac 
        caggccaat gggtgtgct tgccaaaca agggtgatt 
        gaaatgttg ttagacaga ttaatcgcg ttagaaagg 
        caacgaaaa caaccaaca accgaccaa tcttgcaga 
        aatacaaga attcaaccc aacaaaaca tcaacacct 
        tcagactca ttacggttt cgaagcaat cccaaaact 
        tcgggacta aacgttcca attgtgccc agaaacaac 
        ttggacgtc ctccaggat ctccttaaa gactttcct 
        cttcccaaa tacccgaaa gaaaaaatg atgtatcag 
        aaatttaag gcgctatta caaaagcca caagtccaa 
        aaccttgtc aaaaacctg gacttgtcc aagtacaaa 
        ccgaacgaa attctctac atcatcata accacagtt 
        ctgcatact ctcaagact gaggaaata caagaagtt 
        ttcgccttc tttgaaacg tacagagaa gcggcacgg 
        actggcttc aactttggc aacttgtta gatttgtta 
        ccgccaccg aagagctcg aaggagcga aattaccac 
        aacatcacc aaacacttg ctcctcagc agagctctg 
        tacaaccta aacttgggt cccaacttc agacaagcc 
        ccaaacctt gccgacaag ctaataatt ttgctgcaa 
        gaactgctc aaaactaac acaattcct ggggaagtt 
        agagaggcg cttcagtac tacttgccg gtttttttg 
        ggaaagcgg gcaaacatt cacttcgaa aacttgata 
        cctttgtta ccgaaacca gtgaacgac gttgagagg 
        aaacagttg gatgtgttg aagaatctt ctggcaagt 
        aacgagttg tacgtcttg atgaaaaac tacaatttg 
        aataacttg actccggcc caactttta caaatcgtt 
        ctgcaccac ttgatcaac cagcatggt gattattca 
        gacgctgcc ttgtattac agaaacaag ctggcaatt 
        aacaagtat gggtttaat tattacgac ttgcttaac 
        ttgattcct gtgacgcaa gagaaccgg gcgtttgtt 
        caactgctc agtaattat ttccacagt cctgagttt 
        gaagcaatt ctggcactg ccaagcctg aagacgttg 
        tcaaacgtt gataagtta ctcctgatt cttgaaacc 
        atccaaaag aaatcgact gatgctaat gtgcttaaa 
        gcagcagca agtgtgttg gatgacgtc cggttgcaa 
        gtcttctac cagaacata cagcagaaa gttatcggt 
        ctaatccca gcacccaag tccaacatt gaaaaacaa 
        caatacgaa acagtgaaa agcttcctg ttgagcaaa 
        agggcgttc ccgatgttg ggtggaatc gatttaagt 
        gtgttccct gatagtaaa caccttctc gagtttgtt 
        tttaaaaca gtaacggag agtaatttg gagcaaaac 
        attcgggac gctttcgag tactacctg gacatagct 
        cataagttg aaaccgctt gaatttgtg accaagaag 
        gagattaag aaaaagttc gaactgacg gaaattttc 
        accgacact ttggacctt aaaaccctg acagttccg 
        caaaaacgg gcgtttaaa aacttcttt aagtacatt 
        tccgaagtt aaaccggac gccatgtcc aagttcgat 
        tcctgggac caagccaaa actagagga gagtttatg 
        aagttgctt ttcaagtat ttcgtcgag tccccaaca 
        gttcttccc gaaattaag gaaagtatc aagattctt 
        gatcctctg gtcaagatg gacgggaaa ggagcgtta 
        ccaccataa
      mRNA sequence
      • >TC013173 RA
        aggcaggcg gttgtcgat caaacgctg acactgcat 
        tattattcc cgattctct aaaccgcaa acagtaaac 
        aatttttaa tgttattgg attatgagt agacttagt 
        gctggttac aatgtttcg tcaggctaa cagtgtgag 
        ggcactttt cgtgctttg cttgttcaa aatcattgc 
        atgcgatgg ctctttcca ccttttcta tcaaatccc 
        tgcaagtga atatgacgg ctgcgaaaa cctgtcaag 
        attcttcca aacgattca aacatcgtc cccagtcaa 
        tgctctaaa agcgatcat tgcaccact ggcctgcag 
        cacttgtcc gcaagaatc tcacctcct acaccaaaa 
        attaatcga actggcatc acacggctc gttaaatat 
        caactttgt tatcgcaaa tctcaaaaa aatgaaaaa 
        cttgaacag atacgatgt tttaattac cactttgat 
        ggcaattaa cgagtttac aacggttga ccccagctt 
        gaagcttga aatcagcaa cgagatctt ttatttgct 
        caagaggcg gtacttgaa gatcaatag ctactccaa 
        atcacgcca agggttttg tcagtacga tgctgcgaa 
        gccccaagt cggcggaat tttctcaag gcctaagca 
        aacccccat attaattca aacgcccat caggaatga 
        ttatttaag ggctccagt tggtagaat ctcacagcc 
        atttgtgtc ccaaattcc tgttttaat tcaaactcc 
        tatcaagta ctatccaaa ggctgctcc tagttatgt 
        tcctccagt ggttcagca cgtcatggg caaagcttt 
        accgaccaa ccaggccaa tgggtgtgc ttgccaaac 
        aagggtgat tgaaatgtt gttagacag attaatcgc 
        gttagaaag gcaacgaaa acaaccaac aaccgacca 
        atcttgcag aaatacaag aattcaacc caacaaaac 
        atcaacacc ttcagactc attacggtt tcgaagcaa 
        tcccaaaac ttcgggact aaacgttcc aattgtgcc 
        cagaaacaa cttggacgt cctccagga tctccttaa 
        agactttcc tcttcccaa atacccgaa agaaaaaat 
        gatgtatca gaaatttaa ggcgctatt acaaaagcc 
        acaagtcca aaaccttgt caaaaacct ggacttgtc 
        caagtacaa accgaacga aattctcta catcatcat 
        aaccacagt tctgcatac tctcaagac tgaggaaat 
        acaagaagt tttcgcctt ctttgaaac gtacagaga 
        agcggcacg gactggctt caactttgg caacttgtt 
        agatttgtt accgccacc gaagagctc gaaggagcg 
        aaattacca caacatcac caaacactt gctcctcag 
        cagagctct gtacaacct aaacttggg tcccaactt 
        cagacaagc cccaaacct tgccgacaa gctaataat 
        tttgctgca agaactgct caaaactaa cacaattcc 
        tggggaagt tagagaggc gcttcagta ctacttgcc 
        ggttttttt gggaaagcg ggcaaacat tcacttcga 
        aaacttgat acctttgtt accgaaacc agtgaacga 
        cgttgagag gaaacagtt ggatgtgtt gaagaatct 
        tctggcaag taacgagtt gtacgtctt gatgaaaaa 
        ctacaattt gaataactt gactccggc ccaactttt 
        acaaatcgt tctgcacca cttgatcaa ccagcatgg 
        tgattattc agacgctgc cttgtatta cagaaacaa 
        gctggcaat taacaagta tgggtttaa ttattacga 
        cttgcttaa cttgattcc tgtgacgca agagaaccg 
        ggcgtttgt tcaactgct cagtaatta tttccacag 
        tcctgagtt tgaagcaat tctggcact gccaagcct 
        gaagacgtt gtcaaacgt tgataagtt actcctgat 
        tcttgaaac catccaaaa gaaatcgac tgatgctaa 
        tgtgcttaa agcagcagc aagtgtgtt ggatgacgt 
        ccggttgca agtcttcta ccagaacat acagcagaa 
        agttatcgg tctaatccc agcacccaa gtccaacat 
        tgaaaaaca acaatacga aacagtgaa aagcttcct 
        gttgagcaa aagggcgtt cccgatgtt gggtggaat 
        cgatttaag tgtgttccc tgatagtaa acaccttct 
        cgagtttgt ttttaaaac agtaacgga gagtaattt 
        ggagcaaaa cattcggga cgctttcga gtactacct 
        ggacatagc tcataagtt gaaaccgct tgaatttgt 
        gaccaagaa ggagattaa gaaaaagtt cgaactgac 
        ggaaatttt caccgacac tttggacct taaaaccct 
        gacagttcc gcaaaaacg ggcgtttaa aaacttctt 
        taagtacat ttccgaagt taaaccgga cgccatgtc 
        caagttcga ttcctggga ccaagccaa aactagagg 
        agagtttat gaagttgct tttcaagta tttcgtcga 
        gtccccaac agttcttcc cgaaattaa ggaaagtat 
        caagattct tgatcctct ggtcaagat ggacgggaa 
        aggagcgtt accaccata aataaaaaa tagtgtttt 
        tattaaa
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