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Gene information
General information
TC032019
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004465
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 4635699..4636968
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032019
    iBeetle screen iB_11003

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032019 RA
      MFSLPSHVL EKLRCAQCR GYLSSGPIV ANRKNEQLC 
      GRCFKIFPD ERKAEYLRQ IGLEAVAQI IIFPCKYTD 
      QGCTHTFPW NNGLEHELN CEHRYDTLP LMGDPKCDK 
      SNEEIDLKV YCDTTYNHN FEDTAKLSI NLTGKNNNK 
      LALITGRNH QRSVRLGVE VTLSLDATP QILHENVVV 
      EDSIEENLY ESINVFDHC ASCLNNVLN CEVYTCSLG 
      HIACKKCKS PNCRICSFQ LNSNLLQFC KNYVRGCTE 
      LFPAGDIKK HEIDCEFND FNCPLCDSA NNLNILVAH 
      FQQTHNPIC SNEFNAIVT EQDETWFFA CFNKLFRCK 
      YYYLRTSIE FVVTYVGSN DKACDYKYS VTVLPKNLK 
      SVTKQAKCL GWNDASLGK AIVFEVANN RKKFEVRVS 
      ISIVPQV
    CDS sequence
    • >TC032019 RA
      atgttctcg cttccgagc catgttttg gaaaagtta 
      cgctgtgct cagtgccgc ggctattta agctctgga 
      ccaattgta gcaaatcga aaaaacgag caattgtgt 
      ggccgctgt tttaaaata ttcccggat gaaaggaaa 
      gcggagtat ttgcgccaa atcggactt gaagccgta 
      gcgcaaatt atcattttt ccctgtaaa tacaccgac 
      caaggctgc actcacaca tttccatgg aataatggc 
      ctcgaacat gaactcaat tgtgagcac agatacgac 
      actctgcct ttgatggga gaccccaaa tgtgataag 
      agtaacgaa gaaattgat ttaaaagtg tactgcgac 
      acgacctat aatcacaac tttgaagat accgccaaa 
      ttgagcata aacttgacc gggaaaaac aataacaaa 
      ctagcgctg attactgga cgaaaccac caacgaagt 
      gttagactt ggggtcgaa gtaacccta tctctggac 
      gccacaccg caaatcctc cacgaaaac gttgttgtt 
      gaagattcc atcgaagaa aacttatat gaatcaatc 
      aacgtgttc gaccactgc gccagttgc ctcaacaac 
      gtcctcaac tgcgaggtc tacacctgt tccttgggc 
      cacatcgcg tgcaaaaaa tgcaaaagc cccaattgc 
      cggatttgt agttttcaa ttaaactcc aatttgctt 
      cagttttgt aaaaattac gtgcgtggt tgcacggaa 
      ttgttccca gctggtgac ataaaaaag cacgaaatt 
      gactgcgaa tttaacgat ttcaactgt cctttgtgc 
      gattctgca aacaaccta aatattttg gtggcgcat 
      tttcaacaa acgcacaat ccgatttgt tcgaacgaa 
      ttcaatgcc attgttacc gagcaagat gaaacgtgg 
      tttttcgcc tgttttaat aaactgttc cgttgcaaa 
      tattactac ttaaggacc agcattgag ttcgttgtg 
      acttatgtc ggaagcaac gacaaggct tgcgattac 
      aaatacagc gttactgtc ttaccgaag aatttgaaa 
      agtgtcacc aaacaggcg aagtgctta ggctggaac 
      gacgcaagt ttgggcaaa gcgatagtt tttgaagtg 
      gccaataac aggaaaaaa tttgaagtg cgtgtatcc 
      ataagtatt gtaccacaa gtttaa
    mRNA sequence
    • >TC032019 RA
      tgtccttac ctcgtgcga aaatgttct cgcttccga 
      gccatgttt tggaaaagt tacgctgtg ctcagtgcc 
      gcggctatt taagctctg gaccaattg tagcaaatc 
      gaaaaaacg agcaattgt gtggccgct gttttaaaa 
      tattcccgg atgaaagga aagcggagt atttgcgcc 
      aaatcggac ttgaagccg tagcgcaaa ttatcattt 
      ttccctgta aatacaccg accaaggct gcactcaca 
      catttccat ggaataatg gcctcgaac atgaactca 
      attgtgagc acagatacg acactctgc ctttgatgg 
      gagacccca aatgtgata agagtaacg aagaaattg 
      atttaaaag tgtactgcg acacgacct ataatcaca 
      actttgaag ataccgcca aattgagca taaacttga 
      ccgggaaaa acaataaca aactagcgc tgattactg 
      gacgaaacc accaacgaa gtgttagac ttggggtcg 
      aagtaaccc tatctctgg acgccacac cgcaaatcc 
      tccacgaaa acgttgttg ttgaagatt ccatcgaag 
      aaaacttat atgaatcaa tcaacgtgt tcgaccact 
      gcgccagtt gcctcaaca acgtcctca actgcgagg 
      tctacacct gttccttgg gccacatcg cgtgcaaaa 
      aatgcaaaa gccccaatt gccggattt gtagttttc 
      aattaaact ccaatttgc ttcagtttt gtaaaaatt 
      acgtgcgtg gttgcacgg aattgttcc cagctggtg 
      acataaaaa agcacgaaa ttgactgcg aatttaacg 
      atttcaact gtcctttgt gcgattctg caaacaacc 
      taaatattt tggtggcgc attttcaac aaacgcaca 
      atccgattt gttcgaacg aattcaatg ccattgtta 
      ccgagcaag atgaaacgt ggtttttcg cctgtttta 
      ataaactgt tccgttgca aatattact acttaagga 
      ccagcattg agttcgttg tgacttatg tcggaagca 
      acgacaagg cttgcgatt acaaataca gcgttactg 
      tcttaccga agaatttga aaagtgtca ccaaacagg 
      cgaagtgct taggctgga acgacgcaa gtttgggca 
      aagcgatag tttttgaag tggccaata acaggaaaa 
      aatttgaag tgcgtgtat ccataagta ttgtaccac 
      aagtttaat aaaacgagt acttaatta ataaatgtt 
      ttaattggt g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY