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Gene information
General information
TC009674 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3839706..3841263
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC009674
      iBeetle screen iB_11002

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC009674 RA
        MDQLEESHY FHLPSQGNI YTITHLEAA NCPHKILVA 
        SLKRDIFCI EYQESGGCL IPTTKEVSF TYIPSGAEI 
        ISVDAFNKS ATKNEFVIG ITIIKNSNL LDSSFEYYL 
        NIYSEWEES DDFNIDNIA QNCLNVELS FTPYQLTHT 
        DLIIWENEQ ITTKEIVFL LSGSDNQIH IYREYTTDH 
        LYKEVDKEC FPEFIKTPS PVVWIDIYY NDNYSQRVT 
        AMACECGYV RLTQVCVKT NKLSFNFST KLNNYISHL 
        RLYPDEKLI SLAEKPVNC GLNLVVVNT ILPPVLFQN 
        VLVYGLSNY VSLPRHDYT CVLTCCQVA DVDFDGEKE 
        ILVGNSSEE IMLYKRDPD KGWCLEELK MFASPIISM 
        KYVDLIEDG VNELVVLSM KGVHFLQHD FTHVDRVLN 
        EKIKLVNF
      CDS sequence
      • >TC009674 RA
        atggatcaa ctggaagaa agccattac ttccacttg 
        ccgtcccaa ggcaacatt tacacaatc acgcatttg 
        gaggccgct aattgcccc cacaaaatc ctggttgct 
        tcgctcaaa cgcgatatt ttttgtata gagtaccag 
        gaatcaggg ggctgtctc atacccaca acaaaggaa 
        gtctcattc acttatatc ccaagcggt gctgaaatt 
        atttcagtg gacgctttc aacaaatca gcaacgaaa 
        aatgagttc gttatagga attacaata attaaaaac 
        agcaatttg ttggattca tcgtttgaa tattactta 
        aacatttac tcagaatgg gaggagagc gacgatttt 
        aacatcgac aacatcgct caaaattgc ctaaacgtt 
        gagctgagc ttcactcct tatcagctc acacatacg 
        gacctcata atttgggaa aatgagcaa attacgacc 
        aaagaaatt gtctttttg ttatcagga agcgacaat 
        cagatccac atttatcgg gaatatacc accgaccac 
        ttgtataaa gaggtggac aaggagtgc tttcccgaa 
        tttatcaaa acaccaagt cctgtggtc tggattgat 
        atttactac aacgacaat tattcccaa cgagtcaca 
        gcaatggca tgtgagtgt ggatacgtc cgactgact 
        caagtttgt gttaaaaca aacaaactc agttttaat 
        ttctcgacc aaactgaat aattacatt tcacatttg 
        cggctgtat ccagacgag aaattaatc tctctagcc 
        gaaaaaccg gtaaattgt ggcttaaat ttagtcgtt 
        gttaacaca atcttgcct ccagtttta ttccaaaac 
        gttctagtt tacgggtta agtaattac gtctccttg 
        ccccgacac gactacacg tgtgtcttg acttgttgc 
        caagtcgct gatgttgat ttcgacgga gaaaaagaa 
        atcctagtc ggaaatagt tcagaggaa ataatgttg 
        tataaacgt gaccctgac aagggctgg tgcttggag 
        gagttaaaa atgttcgct tcgcctatc atcagtatg 
        aaatacgtg gacttgatc gaggatggg gttaatgag 
        ttggtggtg ttgtccatg aaaggagtg cattttcta 
        caacatgat tttacacat gttgatagg gttttaaac 
        gaaaaaata aagcttgtt aatttttaa
      mRNA sequence
      • >TC009674 RA
        tctccaacc ttaaaattt ccaaaattt tcaatggat 
        caactggaa gaaagccat tacttccac ttgccgtcc 
        caaggcaac atttacaca atcacgcat ttggaggcc 
        gctaattgc ccccacaaa atcctggtt gcttcgctc 
        aaacgcgat attttttgt atagagtac caggaatca 
        gggggctgt ctcataccc acaacaaag gaagtctca 
        ttcacttat atcccaagc ggtgctgaa attatttca 
        gtggacgct ttcaacaaa tcagcaacg aaaaatgag 
        ttcgttata ggaattaca ataattaaa aacagcaat 
        ttgttggat tcatcgttt gaatattac ttaaacatt 
        tactcagaa tgggaggag agcgacgat tttaacatc 
        gacaacatc gctcaaaat tgcctaaac gttgagctg 
        agcttcact ccttatcag ctcacacat acggacctc 
        ataatttgg gaaaatgag caaattacg accaaagaa 
        attgtcttt ttgttatca ggaagcgac aatcagatc 
        cacatttat cgggaatat accaccgac cacttgtat 
        aaagaggtg gacaaggag tgctttccc gaatttatc 
        aaaacacca agtcctgtg gtctggatt gatatttac 
        tacaacgac aattattcc caacgagtc acagcaatg 
        gcatgtgag tgtggatac gtccgactg actcaagtt 
        tgtgttaaa acaaacaaa ctcagtttt aatttctcg 
        accaaactg aataattac atttcacat ttgcggctg 
        tatccagac gagaaatta atctctcta gccgaaaaa 
        ccggtaaat tgtggctta aatttagtc gttgttaac 
        acaatcttg cctccagtt ttattccaa aacgttcta 
        gtttacggg ttaagtaat tacgtctcc ttgccccga 
        cacgactac acgtgtgtc ttgacttgt tgccaagtc 
        gctgatgtt gatttcgac ggagaaaaa gaaatccta 
        gtcggaaat agttcagag gaaataatg ttgtataaa 
        cgtgaccct gacaagggc tggtgcttg gaggagtta 
        aaaatgttc gcttcgcct atcatcagt atgaaatac 
        gtggacttg atcgaggat ggggttaat gagttggtg 
        gtgttgtcc atgaaagga gtgcatttt ctacaacat 
        gattttaca catgttgat agggtttta aacgaaaaa 
        ataaagctt gttaatttt taaatgctg aaggtgggg 
        tctttgacg accactatt ttatcgact gaagttgag 
        ataaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY