Status
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Gene information
General information
TC009383 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1312706..1324891
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009383
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_11001

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009383 RA
      MANPRKFSE KIALHTHRQ AEETARFEQ IMKEVSDAT 
      ARDEATNAN MVNYRPGSR ARSQGGPMR RPHDRRLDT 
      SPYSSNSFL SPPHDTSWR RTHSDSALH QSAMQDMNS 
      ERNDGCNSR WIMPNMNGP ERNEGRPRS SCEVPRVPG 
      IHIYPSAHV PGAVQIPIS GNNTGSLPD LTNVNFSSP 
      IRVPLDQDA DQGSSPYSS SPVNTSPST LSPTSIPQG 
      VRQGRFHFT HVSHAHSNH LSVPRYPNH PSKNVPVNS 
      KSIAGLVDG TDLTQLTGM QGMYQQQCP PTSPSPTLP 
      QASGYRSPR PSPQSSPSL GGRHSAPCS PGAPSPLPN 
      DYNNIFNQH QATQFQQHF EQLSMLDPP VSSVSYVDQ 
      SPGQLTATH AQTVPEVST VGSIELTSD PGYYSTSPS 
      QLVYPPSLH TTPNTPTSI PDIILTDFS SATDDISRQ 
      DLTKQLEND FFSEETLRE SLEPLNFDE LQMLTDPSM 
      NFISDGVED GFRLHRS
    CDS sequence
    • >TC009383 RA
      atggcgaat ccgaggaag ttcagcgag aaaatagcc 
      ctccatacc caccgtcag gcggaggaa acggcccgt 
      ttcgagcag attatgaaa gaggtgtcc gacgcgacg 
      gccagggac gaagcaacc aacgcaaac atggtaaac 
      taccgacct ggaagcagg gcacgttca caaggtggc 
      cccatgcgg aggccacac gaccgaaga ctagacaca 
      tcaccctat tccagtaat tcttttcta agtccacca 
      cacgacacg agttggaga cgtacacat agcgattcg 
      gctctacat caaagcgcc atgcaggac atgaattcg 
      gagcgaaac gacggctgc aacagccgc tggattatg 
      ccaaatatg aacggtcct gaacgaaat gaaggacga 
      ccgaggtcg agttgtgaa gttcctcgt gtacctggt 
      atacatatt tatccttcg gcacatgtg cctggagcg 
      gtgcaaata ccgatcagt ggtaataac acgggttcg 
      ttgcccgac ttgactaat gtgaatttt tcgtcgccg 
      atccgagta cccttagat caagacgca gaccaagga 
      agttccccg tatagctct agtcctgta aatacaagt 
      ccatctaca ttatctccc acaagtata cctcaagga 
      gtacgacaa ggtcgtttt cattttaca catgtatca 
      catgcacac tcgaatcat ttatcagtt cctaggtat 
      ccgaatcat ccaagtaag aatgtgccg gtgaatagc 
      aaaagtata gctggctta gtagacgga acagacctg 
      acccaattg accggaatg cagggaatg tatcagcag 
      cagtgtccg cctacatcg ccctctcca acgttacca 
      caagcgtcc ggttatcga agtcctaga cccagtccc 
      caatcgtca cctagttta ggaggacga cattcggct 
      ccttgtagt cctggagct cctagtcca ctgccgaac 
      gattataat aatatattc aatcaacac caagcgact 
      cagtttcag caacacttc gagcagtta tcgatgttg 
      gacccacct gttagtagc gtatcgtat gtagaccaa 
      tcacccggt cagttaaca gcaacgcat gcccaaact 
      gtgccagaa gtgtcaaca gtgggcagc atagaactg 
      acatccgat ccaggatat tacagcaca tcaccctcc 
      caactcgtt tatccgcca agtttgcac acaacaccg 
      aatactcca accagtata cccgatatt atactaact 
      gatttttca tcggcaact gacgatata tcgcgacaa 
      gacttgacc aagcaactg gagaacgac ttcttctcg 
      gaggagacg ctgcgagag agtctcgaa ccgctgaac 
      tttgacgaa cttcaaatg ctgaccgat cccagcatg 
      aacttcata tcggacgga gtggaggac ggctttaga 
      ctgcatcga tcataa
    mRNA sequence
    • >TC009383 RA
      aacgaaccg gtggctgca ctgtcactc catgtctgc 
      cagtgcgct tattattat ttattattg tgttttgtt 
      tacggctga attgaaata agttccgta aaagtcgtt 
      aaccgaggt cgcccagtg acattgtcg cagtgtcca 
      gcacctccg ccgccgcag atgtgtccc agctcctcc 
      acaacggca acaatgaca ggtcggtcg aggtcgacg 
      acgtttccg taacgtctt gcaaataac gatgttgtg 
      gtgatcgcc agtggcccc agagcagtc ggaaaacgg 
      tcgaacaat cgaaaaagc cgctccaag atatcgact 
      aacgcgacc ctgaaacgc gccgcgcag tgtagtgag 
      caaatgttt agcaatcat ggcgaatcc gaggaagtt 
      cagcgagaa aatagccct ccataccca ccgtcaggc 
      ggaggaaac ggcccgttt cgagcagat tatgaaaga 
      ggtgtccga cgcgacggc cagggacga agcaaccaa 
      cgcaaacat ggtaaacta ccgacctgg aagcagggc 
      acgttcaca aggtggccc catgcggag gccacacga 
      ccgaagact agacacatc accctattc cagtaattc 
      ttttctaag tccaccaca cgacacgag ttggagacg 
      tacacatag cgattcggc tctacatca aagcgccat 
      gcaggacat gaattcgga gcgaaacga cggctgcaa 
      cagccgctg gattatgcc aaatatgaa cggtcctga 
      acgaaatga aggacgacc gaggtcgag ttgtgaagt 
      tcctcgtgt acctggtat acatattta tccttcggc 
      acatgtgcc tggagcggt gcaaatacc gatcagtgg 
      taataacac gggttcgtt gcccgactt gactaatgt 
      gaatttttc gtcgccgat ccgagtacc cttagatca 
      agacgcaga ccaaggaag ttccccgta tagctctag 
      tcctgtaaa tacaagtcc atctacatt atctcccac 
      aagtatacc tcaaggagt acgacaagg tcgttttca 
      ttttacaca tgtatcaca tgcacactc gaatcattt 
      atcagttcc taggtatcc gaatcatcc aagtaagaa 
      tgtgccggt gaatagcaa aagtatagc tggcttagt 
      agacggaac agacctgac ccaattgac cggaatgca 
      gggaatgta tcagcagca gtgtccgcc tacatcgcc 
      ctctccaac gttaccaca agcgtccgg ttatcgaag 
      tcctagacc cagtcccca atcgtcacc tagtttagg 
      aggacgaca ttcggctcc ttgtagtcc tggagctcc 
      tagtccact gccgaacga ttataataa tatattcaa 
      tcaacacca agcgactca gtttcagca acacttcga 
      gcagttatc gatgttgga cccacctgt tagtagcgt 
      atcgtatgt agaccaatc acccggtca gttaacagc 
      aacgcatgc ccaaactgt gccagaagt gtcaacagt 
      gggcagcat agaactgac atccgatcc aggatatta 
      cagcacatc accctccca actcgttta tccgccaag 
      tttgcacac aacaccgaa tactccaac cagtatacc 
      cgatattat actaactga tttttcatc ggcaactga 
      cgatatatc gcgacaaga cttgaccaa gcaactgga 
      gaacgactt cttctcgga ggagacgct gcgagagag 
      tctcgaacc gctgaactt tgacgaact tcaaatgct 
      gaccgatcc cagcatgaa cttcatatc ggacggagt 
      ggaggacgg ctttagact gcatcgatc ataacagtg 
      gtatgtgtc acacactta atttagcgc ccaataaga 
      gcggaattt cagaaattc actgccaaa aatcagtgg 
      gtttgtcaa aagacagca tttcacaaa aattgaatt 
      tattaagtg tgattgtta gattagtgt ttcccagcc 
      aagctgaag gtgcgacgg tttattaat aattcattt 
      ttatagtag aaaaattga tattgcaat tatttcgaa 
      aaaaagttg aacattttt acgaaagac tgaaaaaac 
      agtgtagac tgttttaac gaatttttg gaatacact 
      taaattttt cgtttcgat ttttttacg ttaggatag 
      gaataaata aagcacaag ttttataat gaagacctc 
      ggcaatgtt ttttacata actacataa aactgtact 
      aggactaga tgcataggt gtttgtaca aaatattcg 
      ataagttag tttttttac taaacgtaa ttattttaa 
      aaaggtgat ttcgttatt acggggcaa accgctgac 
      cataaaata aatttacac ctaaattaa agttaaaaa 
      atttggttt aatcatttg taataatta ttcttattg 
      taaaaatat ttttgatgg tttttgggc ttcattcaa 
      taaagaatt aaaaaaaca ctgataata cttaatgca 
      tatcagtag acacaagta ttttgggtt tcagaaaaa 
      aagcaaaaa attaaaatt gtggaaccg atatttgtc 
      ttgcaattt taaaaactt tatgtgaat aaaaagttt 
      gtcagtgat ttaccccgt tattaattt tgattgtgg 
      ggagtcatc gttttaaaa tattgtggg tagccaatt 
      acgtttcag cttactttt aagagtgac attagtgca 
      atataggtt ttttaatta taattttat actttacgt 
      tgagaatac atgttgagt actttaaat ttttatatt 
      gtttgttct ggactgtat agctataga agcgcacaa 
      gttagtcta aagcgaacc tgtttttat attgtccat 
      gcagtttac ttaaatttg gtaattgtc aacaatgtg 
      agtcattaa aatagtgga caatgtaag aattgggag 
      taatccgta tcatacata cacatattc aagtggctc 
      tctctccct tcaatatta aaggaaccg acagttttt 
      attaaagaa tctcaaact ttacaatgt tctaaagca 
      aataattat ctctgtgaa atccagttg ttctagagt 
      agtatttgt gaggtgtgt agagtgtgg tgctattta 
      gtcgcgata tggaatgtt gtattttca catatcacg 
      actcgaagc tggctaatt cgttttgtg gagaaacga 
      cccatatcc cagctgtgt taagtgtag atactttta 
      tcaaatcat cacacaagt tggagggat tgatgtttt 
      ttcgagtcg acgttgtgt tcatagtca attatgtca 
      attttgttg aattttctt ggtcaaatt attcagtct 
      tttcagtgc tcaagcgag agagttttt gtacaggtt 
      atgggaacg ctgactaca gtttcatgg aataaaaga 
      cacttttgt atatttgct tatgttgta gtcaaagtg 
      tgaagatca aactaagtg gacctaagt catttttct 
      ctaggtgat agctgactg gtttgttcg atgtagcag 
      atcgtttca ttgtatata ttttttgag tacttgata 
      tatcaatag gtttctaaa tgctatatc gactaaatc 
      tcgtttaat ggagtaacc agttcatta cttaccttt 
      atctcatct tttgtacat attagccat taaatatct 
      gatagattt atatacata ctttatttt agattaaat 
      cttattcag acacaaacg tcaattgtt gcatgcttc 
      gttgttatt tttttaaat tttaaaaat atatttcat 
      gatttgta
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