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Gene information
General information
TC011790 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13472784..13475994
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC011790
      iBeetle screen iB_10999

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC011790 RA
        MVTRCVVQG CRSTSDSSK EKVSFFHVP KNKVELWQK 
        IIRTKQRPL TTKDRICEK HFKKSDIIK EIVTPLYSY 
        KLARNRLKP NALPSMLPI YRNYELRSR SSAMEPPIK 
        KNKLEQPPI EFIEIDESD KLETTPEEV FRSGLQIAR 
        LNYNHSKSL TLEQYDQLV AKVLEAKKR KDDTVLSKF 
        NLVDINGQK KLIATHPTD LQYYVHDDI LFQILQHTH 
        IALQHGNQT QMMSELKKR YKNITGGDV ATFIKFCPR 
        CLRRRPRKD SENVPMIYS KISSICHVN LLDNRAWPD 
        GDYKYILVY HNEMTNFCQ LKALKTKKP SEVAEHLID 
        VFTFVGAPT EIHTNEGES NFLEDILKE LKKIWPSLV 
        IKSVERHQQ EILARIKRI EYETRSIRN RTRWSSLLP 
        FIQFERNKD ISSGVRPYE RLFGFVEPC EKPALPVQK 
        VETPSVEKI DQGIPRFFL PPFFKIPKP TKTNKALEE 
        AFKNLVRDN EAVLTKADY LKLIQQVKV YRNLEQYDT 
        LEIEGVVRL ITSTTHGKG TIKYYIHED ELFDILHYT 
        HINYDHASY TEMAKKFKK KFENITKEI IAAYLSLCP 
        ECNTGMYYI NYGSPTKVV RETIPVNEM SAKCAISFL 
        DFEAQPDND YKYVMVYVN MLTKFHILR PLKDKTCQE 
        VADILLDIF TFLGISTKI FTQERRLVW LAISQVRQL 
        WPGFSPLVV QIRLGRIPR WIEKELKQR MCSNESHEW 
        TKGLDYIQL MLNKYYNNK IGKKPFDAL FAVTAGQDK 
        RPQTIYSSQ DPCTPEVEE VTIPAPDLE EPPQIKKRR 
        RTRRKTPFK VLQTFAMSE LSSRCSVNL IDYHTKPDG 
        DYNFIMVYQ NTSTKFSIL KPLKTKFPE EIAENLVDI 
        FTLVGASNV VESYHGREF VHKLAKSVS HLHPNLTFD 
        YSLTTYRNY KSVCSIEKE IQKWMLQNG SKQWTRALP 
        FVQLVRNKC FDEEMAIVP FEGFFKGLR YAGR
      CDS sequence
      • >TC011790 RA
        atggtaaca agatgtgta gtccaaggt tgtagaagt 
        acgtcggac tccagtaaa gaaaaagtt tcgtttttt 
        catgtccct aaaaataaa gtagagctg tggcaaaaa 
        ataattcgg accaaacaa cggcctctg acgactaaa 
        gacaggatc tgtgagaaa catttcaaa aaatcggac 
        attattaaa gaaattgta acacccctt tactcgtac 
        aaattggcc agaaatcga ctgaaaccc aacgcatta 
        ccatcgatg ttacccatt taccgtaat tacgaactt 
        cggtcgcgg agttcagcc atggagccc cctatcaaa 
        aaaaacaaa ctcgaacaa ccacccatc gagtttatc 
        gaaatagac gaatcggac aaacttgaa acaacacct 
        gaagaagtt ttcaggtcc gggttacaa atagcacgt 
        ttaaattac aatcattcc aaaagcttg actttagag 
        caatacgac caattggtg gctaaggtt ttggaagcg 
        aaaaaaaga aaagacgat actgtatta tcaaaattt 
        aatttagtt gatataaat gggcaaaaa aaattaata 
        gcgacacat ccgactgac cttcagtat tacgtccac 
        gacgatatt ctttttcaa attttgcaa cacactcac 
        atcgcttta caacatgga aatcagacg caaatgatg 
        tcagaattg aaaaaacgc tacaagaat attaccggt 
        ggtgacgtg gccactttt atcaagttc tgtccccga 
        tgtttacga cgacgtcca agaaaagac agcgaaaac 
        gtaccaatg atttacagt aaaattagc tcgatttgt 
        catgtaaac ttattagat aatcgggct tggcctgac 
        ggtgattat aaatatatt ctagtctat cacaacgaa 
        atgacaaat ttttgtcaa ttgaaagca ctaaaaaca 
        aaaaaacct agcgaagtt gccgaacat ttaatcgat 
        gttttcaca tttgttggg gcccccact gaaatccac 
        acaaacgag ggcgaatcc aattttctc gaagacatt 
        ctgaaagaa ttgaagaaa atttggcca agtttggtc 
        ataaaatcg gtcgagcgt caccaacag gaaattcta 
        gccagaatc aaacgcatc gaatacgaa acaagatcg 
        attcgaaat cgcacacgg tggagttcg ctattgccg 
        ttcattcag tttgagaga aacaaagac atctcgtcg 
        ggggtgcgg ccatatgag agacttttc gggtttgtg 
        gaaccctgc gaaaaaccg gccttgcct gtgcaaaag 
        gtcgaaacc ccgagtgtg gaaaaaatc gaccaggga 
        atcccccga ttttttctc ccgccattt ttcaaaatc 
        cccaaaccg acaaaaacg aacaaagcg ctcgaggaa 
        gctttcaaa aacttggtt cgagacaac gaagccgtt 
        ttgacgaag gcggattat ttgaaatta atccaacaa 
        gttaaagtt taccgaaac ttggaacaa tatgacact 
        ctcgaaatc gaaggagtt gtacggctt attacttct 
        accactcat gggaagggc accattaaa tattacatc 
        cacgaagac gaacttttc gatatactc cactacact 
        cacattaac tacgatcac gcaagttac accgagatg 
        gcaaagaaa tttaagaaa aaattcgaa aatataaca 
        aaggagatc attgccgct tacctgtcc ttatgcccg 
        gagtgtaac acaggcatg tattacatt aattacggg 
        tcaccgact aaggtggtt cgtgaaact attcctgtc 
        aatgagatg tctgcgaaa tgtgccatc agttttctg 
        gattttgag gcgcaacct gacaatgat tacaaatat 
        gtgatggtt tatgtaaac atgctgaca aaatttcac 
        atccttaga ccgttgaaa gataaaacg tgtcaagaa 
        gttgcggat attttgtta gacattttt actttcttg 
        ggcatttcg acaaaaatc ttcacacag gagagacgt 
        ttagtgtgg cttgctatc tcgcaagtg agacaatta 
        tggcccgga ttttcacca cttgttgtt caaataaga 
        ctaggtcgt attcctaga tggatcgaa aaagaattg 
        aaacaaagg atgtgcagt aacgagtca cacgaatgg 
        actaaagga ctcgactac atccaacta atgctaaac 
        aagtactac aataataag attgggaaa aagcccttt 
        gacgcactc tttgctgtg accgcaggc caggacaaa 
        cggccccaa acgatatat tcctcccag gacccatgt 
        actcccgaa gtggaagaa gtaacaatt ccagcaccc 
        gacttggaa gaacctccc caaatcaaa aaacgaaga 
        cgaactcgt cgaaaaacg ccctttaaa gttctccaa 
        accttcgca atgagtgaa ctatcgtcc cgttgttcc 
        gtcaacctg atcgactat cacacgaaa ccagacggc 
        gactacaac tttatcatg gtttatcaa aacacttcg 
        acaaaattc tcgattttg aaacccctc aaaacaaaa 
        tttccggaa gaaatcgcc gaaaatcta gtcgatatt 
        tttacccta gttggtgcc tctaacgtg gtcgagtcg 
        taccacggc cgagagttt gttcataaa ttggcaaaa 
        agtgtcagt catctccac ccgaattta acttttgac 
        tacagcttg accacatat cgaaattac aaaagtgtg 
        tgtagtatt gaaaaagaa attcagaaa tggatgttg 
        caaaatggg tcaaaacag tggacacgt gcgcttcct 
        tttgtacaa ttagttagg aataagtgt tttgatgaa 
        gagatggct attgtgcct tttgaggga tttttcaaa 
        gggttgaga tacgcaggg agatag
      mRNA sequence
      • >TC011790 RA
        gtgtgaata tgaaaaaat gagtaattg cataacctc 
        aaagtgtgc gaatgagac aaaacaagt tgcccagct 
        tcacattta aatttccaa gttacactc aatgtaacg 
        ctgaaaatg gtaacaaga tgtgtagtc caaggttgt 
        agaagtacg tcggactcc agtaaagaa aaagtttcg 
        ttttttcat gtccctaaa aataaagta gagctgtgg 
        caaaaaata attcggacc aaacaacgg cctctgacg 
        actaaagac aggatctgt gagaaacat ttcaaaaaa 
        tcggacatt attaaagaa attgtaaca cccctttac 
        tcgtacaaa ttggccaga aatcgactg aaacccaac 
        gcattacca tcgatgtta cccatttac cgtaattac 
        gaacttcgg tcgcggagt tcagccatg gagccccct 
        atcaaaaaa aacaaactc gaacaacca cccatcgag 
        tttatcgaa atagacgaa tcggacaaa cttgaaaca 
        acacctgaa gaagttttc aggtccggg ttacaaata 
        gcacgttta aattacaat cattccaaa agcttgact 
        ttagagcaa tacgaccaa ttggtggct aaggttttg 
        gaagcgaaa aaaagaaaa gacgatact gtattatca 
        aaatttaat ttagttgat ataaatggg caaaaaaaa 
        ttaatagcg acacatccg actgacctt cagtattac 
        gtccacgac gatattctt tttcaaatt ttgcaacac 
        actcacatc gctttacaa catggaaat cagacgcaa 
        atgatgtca gaattgaaa aaacgctac aagaatatt 
        accggtggt gacgtggcc acttttatc aagttctgt 
        ccccgatgt ttacgacga cgtccaaga aaagacagc 
        gaaaacgta ccaatgatt tacagtaaa attagctcg 
        atttgtcat gtaaactta ttagataat cgggcttgg 
        cctgacggt gattataaa tatattcta gtctatcac 
        aacgaaatg acaaatttt tgtcaattg aaagcacta 
        aaaacaaaa aaacctagc gaagttgcc gaacattta 
        atcgatgtt ttcacattt gttggggcc cccactgaa 
        atccacaca aacgagggc gaatccaat tttctcgaa 
        gacattctg aaagaattg aagaaaatt tggccaagt 
        ttggtcata aaatcggtc gagcgtcac caacaggaa 
        attctagcc agaatcaaa cgcatcgaa tacgaaaca 
        agatcgatt cgaaatcgc acacggtgg agttcgcta 
        ttgccgttc attcagttt gagagaaac aaagacatc 
        tcgtcgggg gtgcggcca tatgagaga cttttcggg 
        tttgtggaa ccctgcgaa aaaccggcc ttgcctgtg 
        caaaaggtc gaaaccccg agtgtggaa aaaatcgac 
        cagggaatc ccccgattt tttctcccg ccatttttc 
        aaaatcccc aaaccgaca aaaacgaac aaagcgctc 
        gaggaagct ttcaaaaac ttggttcga gacaacgaa 
        gccgttttg acgaaggcg gattatttg aaattaatc 
        caacaagtt aaagtttac cgaaacttg gaacaatat 
        gacactctc gaaatcgaa ggagttgta cggcttatt 
        acttctacc actcatggg aagggcacc attaaatat 
        tacatccac gaagacgaa cttttcgat atactccac 
        tacactcac attaactac gatcacgca agttacacc 
        gagatggca aagaaattt aagaaaaaa ttcgaaaat 
        ataacaaag gagatcatt gccgcttac ctgtcctta 
        tgcccggag tgtaacaca ggcatgtat tacattaat 
        tacgggtca ccgactaag gtggttcgt gaaactatt 
        cctgtcaat gagatgtct gcgaaatgt gccatcagt 
        tttctggat tttgaggcg caacctgac aatgattac 
        aaatatgtg atggtttat gtaaacatg ctgacaaaa 
        tttcacatc cttagaccg ttgaaagat aaaacgtgt 
        caagaagtt gcggatatt ttgttagac atttttact 
        ttcttgggc atttcgaca aaaatcttc acacaggag 
        agacgttta gtgtggctt gctatctcg caagtgaga 
        caattatgg cccggattt tcaccactt gttgttcaa 
        ataagacta ggtcgtatt cctagatgg atcgaaaaa 
        gaattgaaa caaaggatg tgcagtaac gagtcacac 
        gaatggact aaaggactc gactacatc caactaatg 
        ctaaacaag tactacaat aataagatt gggaaaaag 
        ccctttgac gcactcttt gctgtgacc gcaggccag 
        gacaaacgg ccccaaacg atatattcc tcccaggac 
        ccatgtact cccgaagtg gaagaagta acaattcca 
        gcacccgac ttggaagaa cctccccaa atcaaaaaa 
        cgaagacga actcgtcga aaaacgccc tttaaagtt 
        ctccaaacc ttcgcaatg agtgaacta tcgtcccgt 
        tgttccgtc aacctgatc gactatcac acgaaacca 
        gacggcgac tacaacttt atcatggtt tatcaaaac 
        acttcgaca aaattctcg attttgaaa cccctcaaa 
        acaaaattt ccggaagaa atcgccgaa aatctagtc 
        gatattttt accctagtt ggtgcctct aacgtggtc 
        gagtcgtac cacggccga gagtttgtt cataaattg 
        gcaaaaagt gtcagtcat ctccacccg aatttaact 
        tttgactac agcttgacc acatatcga aattacaaa 
        agtgtgtgt agtattgaa aaagaaatt cagaaatgg 
        atgttgcaa aatgggtca aaacagtgg acacgtgcg 
        cttcctttt gtacaatta gttaggaat aagtgtttt 
        gatgaagag atggctatt gtgcctttt gagggattt 
        ttcaaaggg ttgagatac gcagggaga tagtcttca 
        atagtgtta ttttactac agttatcat tgtgattaa 
        taaattaca atacaatcg
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY