Status
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Gene information
General information
TC004746 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6328236..6333572
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004746
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10998

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004746 RA
      MNKLCVFLF VFFYVVLRA TSDNILGCG GFVKSHVPI 
      DFSKVEVKL LTKQGILKD KINCAPNNG YYFVPLYDK 
      GEYILELEP PPGWSFTPT RVDLVVDGV TDLCSQGKD 
      IDFNFKGFG ITGRVESLG SDSGPEGVE VELKSSSEV 
      RKTTTSSGG SFFFTPVYP GNYVVKIST SKWKVYKDS 
      VNVVVAEGN TELAAKSLI IQGYDVVGA VKDEGEPIK 
      DTTVVLYAQ TSNTDLKID GCDKSPLSG LKSANRPLC 
      HVKTNDQGV FTFGTLPYG KYYVAPFYI EQNIYYQPD 
      SIPFTVEHG SVKLKENFE IIGFNVNGR VLKSSNGKP 
      LPKARVFLN GKEVTQTDS NGVYKLQRL KAGTYNLHV 
      IADNFLFKE VAVKMNPNS RIPDLVPTS YQVCGSVVS 
      DKSQSVTFA QIGSTKVIT TLSDMNSGQ FCEYLSPGK 
      YQVQVVVDS ADSQKGMQF FPKVQNIEV SSEQVGSII 
      FSQLKATVT GKVQCISKK DCQGLKAIL KPSGEKNEI 
      VTNISDDSY KIADIYPGI YEITISDNK LCWKSNKQT 
      VNVNNINVE VPTFVQVGY SVVFSSSHD TQVTYKIPG 
      QNQENTIKI NKGKLAYCL EKAGAYTFH LKSCHSYES 
      SSVSYNTDS ATNEIFLNA QKHTVTLLI ESETKHGDV 
      TATINLDGV KTQSPPLPF VKNGYEIQL LLSPSETAV 
      IIPQSDVLY FSPPILSIS GSTDCENLG AKFKAVLGV 
      VFQGKIIPP LPGVLVTVE TENFDTLMA ETDANGVYK 
      FPPLDKAKS YKIAAKKDS YVLVGPNND GNFLAHKLA 
      EIVIEVVDK NDNAPLQGA LLSLSGGDS YRSNLQTNE 
      NGKITFHSL SPGEYFLRP MMKEYSFEP TSKIIKVNE 
      GQTVNVKLT GKRVAYSAY GQVTSLNRE PEENIVVVA 
      LGVGNCSHF SEESTSESS GLFRIRGLQ PFCSYDITV 
      KSSPNAIER SAPKVVHVE RVSQDIHGL QLVIFRPAT 
      HMDLLVKVY AKNPEHYKS LRLKVVCET ASSGIVYTG 
      RVDTSSIAI TADYNQGVL VQVPPLPLD GKTYSVHLE 
      SNLNNKLEP EPETFIANS SFKYVELDF IVKSSMVEQ 
      EIKQTSIWT LVLIFSIMF AVYNIDKVS QFLKEMLGG 
      YVSDFLNSA NKKSATSDY SGDNDDIDQ IVQSINAVK 
      RKPKPKKN
    CDS sequence
    • >TC004746 RA
      atgaataaa ctatgcgtg tttttgttc gttttcttt 
      tacgttgtg ctccgggca acgtccgat aacatcctg 
      ggatgcggg ggcttcgtt aaaagccac gtcccgatt 
      gacttttcc aaagtggaa gtaaaattg cttacgaag 
      caggggatt ctcaaagac aagatcaac tgcgccccc 
      aataacggc tactacttc gtccctttg tacgacaaa 
      ggcgaatac attttggag ctggaacct ccgcccggc 
      tggagcttc actccgact agggtcgac ttagtcgtc 
      gacggcgtt acggacctc tgcagtcag ggcaaagac 
      atcgatttt aatttcaaa gggttcggg atcactgga 
      agggttgaa agcttgggg agcgacagt ggccctgaa 
      ggggtcgag gtcgagtta aagtctagc tcggaagtg 
      cggaaaact acgactagc agtgggggc agctttttt 
      ttcaccccc gtgtatccc ggcaattac gtcgtgaaa 
      atttccacc tccaaatgg aaagtttat aaagacagt 
      gttaatgtt gtggtggct gaaggcaac acagaattg 
      gctgcgaaa agcttgatt atacaaggg tatgacgtc 
      gtgggggcg gttaaggac gaaggggag ccaatcaag 
      gacacgact gttgtcctg tatgctcaa acatctaac 
      actgactta aaaatcgat ggttgtgat aaatcgccc 
      ctgtctggt ttaaaatca gccaatagg ccgctgtgt 
      cacgttaaa acaaacgac cagggggtg tttactttt 
      ggcactttg ccttatggg aaatattac gttgccccg 
      ttttacatt gaacagaac atttattac cagcctgat 
      tccattccg ttcactgtc gagcacgga agtgtgaaa 
      cttaaggag aattttgag atcatcggt tttaacgta 
      aatgggcgt gtcttgaag tcgtctaat ggcaagccc 
      ttacctaaa gctagagta tttctaaac ggcaaagaa 
      gtaactcag actgattca aacggggtt tataagctt 
      caaagactg aaagctggg acgtacaac ttacacgtg 
      attgccgat aatttcctc tttaaagaa gttgcggtt 
      aaaatgaac cctaacagt cgaatcccg gatttggta 
      ccaacatcg taccaagtt tgcggcagt gttgtcagt 
      gataagtcg caaagcgtc acttttgca caaattggg 
      agtaccaaa gtcattaca acgttatca gatatgaat 
      tcgggtcag ttttgcgag tacttaagt ccggggaag 
      taccaagtt caagtggtg gttgatagc gcagattca 
      caaaaaggg atgcaattc ttccccaaa gtgcagaac 
      attgaagtt agctcggag caagtcggt tctattatt 
      ttttcgcag ctaaaagcc acagtcacc ggaaaagtt 
      cagtgcatt agtaagaag gactgtcaa gggttgaag 
      gccattctg aagcctagt ggcgaaaaa aacgaaatc 
      gtaactaac atttctgac gactcttac aaaattgcc 
      gacatatat cccggaatt tacgagatc acaataagc 
      gataacaag ttgtgttgg aagtctaat aagcaaaca 
      gtcaacgtc aataatatc aacgttgaa gttcctact 
      tttgtccaa gttggctat tcggtcgta tttagttcg 
      tctcatgat acacaggtc acctataaa atccccggt 
      caaaatcag gaaaatacg atcaaaata aacaaaggc 
      aaactagct tattgctta gaaaaggct ggggcttac 
      acatttcac ttaaagagt tgtcacagt tacgaatcc 
      tcttctgtg agctataac actgattcg gcaacaaac 
      gaaatattt ttgaatgcg caaaaacac accgtgact 
      ttactgatc gagtctgag accaaacac ggtgatgta 
      actgcgacc attaatctg gacggagta aaaacccaa 
      agtccacct cttccgttt gttaaaaac ggttacgaa 
      atccaatta ttgctaagt ccgagcgaa acggccgtg 
      attattccc caatcggat gttttatat tttagcccc 
      cccattctt tcaattagt ggcagtacc gactgcgaa 
      aatttaggg gctaagttt aaagctgtc ttaggggtg 
      gtgttccaa gggaaaata ataccgccc cttccgggg 
      gtcctagtc accgtcgag accgaaaat ttcgacact 
      ctaatggca gaaacggac gccaacgga gtttataaa 
      ttcccacct ttggacaaa gccaagtct tacaaaatt 
      gcagcaaag aaagattcc tacgtttta gttggaccg 
      aacaatgac gggaatttc ctcgcacac aaattggcc 
      gaaatcgtt atcgaagtg gttgataag aacgacaat 
      gccccccta caaggagct ttactttcg ctgtcaggc 
      ggggacagt taccggagc aacttgcag acgaacgag 
      aacgggaaa attactttc cactctcta agcccggga 
      gagtacttc ttgcggccg atgatgaaa gagtacagt 
      ttcgagccg acctcgaaa atcatcaaa gtcaacgag 
      ggccaaacc gtcaacgtg aagctaact ggtaaacgg 
      gtcgcttac agcgcctac ggccaggtg acttcgctg 
      aaccgcgaa ccggaggaa aacatagtg gttgtggcg 
      ctaggtgtc ggcaattgc agccacttt tcggaggag 
      tcaacgtcg gaaagttcg gggttgttc cgaatccgc 
      ggtctccag cccttctgc tcgtacgac atcaccgtc 
      aagagcagt ccgaatgca atcgaacgg agcgcccct 
      aaggtcgtc cacgtggag cgggtgtcc caggacatc 
      cacggcttg cagcttgtg attttccgg ccggcgacc 
      cacatggac ttgcttgtg aaggtttac gccaaaaat 
      cccgagcac tacaagagc ttgaggctc aaagttgtg 
      tgcgagacg gcttcctcg gggattgtt tacacaggg 
      cgtgtggat acgtcctcg attgcgatc actgctgat 
      tacaaccaa ggggtttta gtgcaagtg ccaccgttg 
      ccgctggac gggaaaacc tactcagtg cacttagaa 
      tctaacttg aataataag ctcgaacct gaacctgaa 
      actttcatc gcgaattcg tcttttaaa tacgtcgag 
      cttgatttt atcgtcaaa agtagcatg gttgagcag 
      gagattaag caaacatcg atttggacg cttgtcttg 
      atttttagt atcatgttc gcggtttac aatatcgat 
      aaagttagt cagtttttg aaagaaatg ttgggcgga 
      tacgtatca gattttctc aattctgcc aataaaaaa 
      tccgcgact agtgattac agtggcgat aatgatgat 
      attgatcaa attgttcaa agtattaat gctgtgaaa 
      agaaaaccc aaacctaag aaaaattaa
    mRNA sequence
    • >TC004746 RA
      attgtttaa aatgatagt gataaacaa aaataaaaa 
      ttcaaattt ttgcgccac acaatacat tgggatctg 
      aaccgcgga agcaaaatc cctaaatcc gacatgtca 
      aaaattatc tctgttatt taaattgaa ttaatttcg 
      taaaaatga ataaactat gcgtgtttt tgttcgttt 
      tcttttacg ttgtgctcc gggcaacgt ccgataaca 
      tcctgggat gcgggggct tcgttaaaa gccacgtcc 
      cgattgact tttccaaag tggaagtaa aattgctta 
      cgaagcagg ggattctca aagacaaga tcaactgcg 
      cccccaata acggctact acttcgtcc ctttgtacg 
      acaaaggcg aatacattt tggagctgg aacctccgc 
      ccggctgga gcttcactc cgactaggg tcgacttag 
      tcgtcgacg gcgttacgg acctctgca gtcagggca 
      aagacatcg attttaatt tcaaagggt tcgggatca 
      ctggaaggg ttgaaagct tggggagcg acagtggcc 
      ctgaagggg tcgaggtcg agttaaagt ctagctcgg 
      aagtgcgga aaactacga ctagcagtg ggggcagct 
      tttttttca cccccgtgt atcccggca attacgtcg 
      tgaaaattt ccacctcca aatggaaag tttataaag 
      acagtgtta atgttgtgg tggctgaag gcaacacag 
      aattggctg cgaaaagct tgattatac aagggtatg 
      acgtcgtgg gggcggtta aggacgaag gggagccaa 
      tcaaggaca cgactgttg tcctgtatg ctcaaacat 
      ctaacactg acttaaaaa tcgatggtt gtgataaat 
      cgcccctgt ctggtttaa aatcagcca ataggccgc 
      tgtgtcacg ttaaaacaa acgaccagg gggtgttta 
      cttttggca ctttgcctt atgggaaat attacgttg 
      ccccgtttt acattgaac agaacattt attaccagc 
      ctgattcca ttccgttca ctgtcgagc acggaagtg 
      tgaaactta aggagaatt ttgagatca tcggtttta 
      acgtaaatg ggcgtgtct tgaagtcgt ctaatggca 
      agcccttac ctaaagcta gagtatttc taaacggca 
      aagaagtaa ctcagactg attcaaacg gggtttata 
      agcttcaaa gactgaaag ctgggacgt acaacttac 
      acgtgattg ccgataatt tcctcttta aagaagttg 
      cggttaaaa tgaacccta acagtcgaa tcccggatt 
      tggtaccaa catcgtacc aagtttgcg gcagtgttg 
      tcagtgata agtcgcaaa gcgtcactt ttgcacaaa 
      ttgggagta ccaaagtca ttacaacgt tatcagata 
      tgaattcgg gtcagtttt gcgagtact taagtccgg 
      ggaagtacc aagttcaag tggtggttg atagcgcag 
      attcacaaa aagggatgc aattcttcc ccaaagtgc 
      agaacattg aagttagct cggagcaag tcggttcta 
      ttatttttt cgcagctaa aagccacag tcaccggaa 
      aagttcagt gcattagta agaaggact gtcaagggt 
      tgaaggcca ttctgaagc ctagtggcg aaaaaaacg 
      aaatcgtaa ctaacattt ctgacgact cttacaaaa 
      ttgccgaca tatatcccg gaatttacg agatcacaa 
      taagcgata acaagttgt gttggaagt ctaataagc 
      aaacagtca acgtcaata atatcaacg ttgaagttc 
      ctacttttg tccaagttg gctattcgg tcgtattta 
      gttcgtctc atgatacac aggtcacct ataaaatcc 
      ccggtcaaa atcaggaaa atacgatca aaataaaca 
      aaggcaaac tagcttatt gcttagaaa aggctgggg 
      cttacacat ttcacttaa agagttgtc acagttacg 
      aatcctctt ctgtgagct ataacactg attcggcaa 
      caaacgaaa tatttttga atgcgcaaa aacacaccg 
      tgactttac tgatcgagt ctgagacca aacacggtg 
      atgtaactg cgaccatta atctggacg gagtaaaaa 
      cccaaagtc cacctcttc cgtttgtta aaaacggtt 
      acgaaatcc aattattgc taagtccga gcgaaacgg 
      ccgtgatta ttccccaat cggatgttt tatatttta 
      gccccccca ttctttcaa ttagtggca gtaccgact 
      gcgaaaatt taggggcta agtttaaag ctgtcttag 
      gggtggtgt tccaaggga aaataatac cgccccttc 
      cgggggtcc tagtcaccg tcgagaccg aaaatttcg 
      acactctaa tggcagaaa cggacgcca acggagttt 
      ataaattcc cacctttgg acaaagcca agtcttaca 
      aaattgcag caaagaaag attcctacg ttttagttg 
      gaccgaaca atgacggga atttcctcg cacacaaat 
      tggccgaaa tcgttatcg aagtggttg ataagaacg 
      acaatgccc ccctacaag gagctttac tttcgctgt 
      caggcgggg acagttacc ggagcaact tgcagacga 
      acgagaacg ggaaaatta ctttccact ctctaagcc 
      cgggagagt acttcttgc ggccgatga tgaaagagt 
      acagtttcg agccgacct cgaaaatca tcaaagtca 
      acgagggcc aaaccgtca acgtgaagc taactggta 
      aacgggtcg cttacagcg cctacggcc aggtgactt 
      cgctgaacc gcgaaccgg aggaaaaca tagtggttg 
      tggcgctag gtgtcggca attgcagcc acttttcgg 
      aggagtcaa cgtcggaaa gttcggggt tgttccgaa 
      tccgcggtc tccagccct tctgctcgt acgacatca 
      ccgtcaaga gcagtccga atgcaatcg aacggagcg 
      cccctaagg tcgtccacg tggagcggg tgtcccagg 
      acatccacg gcttgcagc ttgtgattt tccggccgg 
      cgacccaca tggacttgc ttgtgaagg tttacgcca 
      aaaatcccg agcactaca agagcttga ggctcaaag 
      ttgtgtgcg agacggctt cctcgggga ttgtttaca 
      cagggcgtg tggatacgt cctcgattg cgatcactg 
      ctgattaca accaagggg ttttagtgc aagtgccac 
      cgttgccgc tggacggga aaacctact cagtgcact 
      tagaatcta acttgaata ataagctcg aacctgaac 
      ctgaaactt tcatcgcga attcgtctt ttaaatacg 
      tcgagcttg attttatcg tcaaaagta gcatggttg 
      agcaggaga ttaagcaaa catcgattt ggacgcttg 
      tcttgattt ttagtatca tgttcgcgg tttacaata 
      tcgataaag ttagtcagt ttttgaaag aaatgttgg 
      gcggatacg tatcagatt ttctcaatt ctgccaata 
      aaaaatccg cgactagtg attacagtg gcgataatg 
      atgatattg atcaaattg ttcaaagta ttaatgctg 
      tgaaaagaa aacccaaac ctaagaaaa attaaatgt 
      tatgtggaa ttttgttta ataaaggtt tgttactca 
      atcgctgtt tttatcaca tttcctaac acacacttc 
      cctttatat ttcgcgcac gcaagtcgg aaaagcagt 
      tttacagga tgcaaattt gatttgttg gaagaagag 
      ctgttacag ataaaaggt ttgtgacag gtagttgaa 
      ataattata caataaagt atgaaacaa tca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY