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Gene information
General information
TC009994 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10582449..10584932
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009994
    iBeetle screen iB_10997

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009994 RA
      MADVRDIME LEHPASQEV TRESIIGSD KQRKRPPTT 
      TKTQKRPEG MHREVFALL YNDNKDVSP LFPSDTGHG 
      YKQTKIKLG MRKPRKWKW VPFTNPART DGAVFHHWR 
      RPSDEPKEY PFAKFNKKV DICTYTDAE YQQHLRVDG 
      WTKDETDHM MQLAQRFDL RFILMADRY DTEKFPKRS 
      VEDIKDRYY KICGIMSKL RGEKKIYTY DVDHEKRRK 
      EQLKKLYDR TQEQIEEEQ FLLLELKKI EARKKERER 
      KTQDLQKLI SQADSQSET PRKTDKKLP KKKIANPSR 
      PSRVDTAHI LQAVETAGI KFPDYKNSG VSLRSQRMK 
      LPANVGQKK SKGIEQMLQ EIGLELNPI PTEEICQNF 
      NELRSDMVL LMEIKSALS TCEFELQSL RHQYEALNP 
      GKTLTIPPQ LISNLDLEN KAGTGEIID VVGSPSTPT 
      S
    CDS sequence
    • >TC009994 RA
      atggccgat gttcgcgat attatggag ctggagcat 
      ccagcgagc caagaggtg actcgagag tcaatcatt 
      ggttcagac aagcagagg aagcggccc ccaactact 
      acaaaaacc cagaaaagg cctgaggga atgcaccgt 
      gaagttttc gccttgttg tacaatgat aataaggac 
      gtttctccg ctttttccg tctgataca ggacatggg 
      tataaacaa acgaaaatt aagttgggc atgaggaaa 
      ccgagaaag tggaagtgg gtgccgttt acgaacccg 
      gcaaggacc gatggggcg gtgtttcat cactggagg 
      aggccgtcc gacgaacct aaggaatat ccttttgcc 
      aagtttaac aagaaagtt gatatttgt acatacact 
      gatgccgaa taccagcag cacttaaga gttgatggt 
      tggaccaag gacgaaact gaccacatg atgcaactg 
      gcacagaga ttcgatctc aggtttatc ctaatggct 
      gatagatat gacactgaa aagtttccg aaaagatca 
      gtggaggat attaaagac cgttactat aaaatttgc 
      ggcatcatg agcaaatta agaggggag aaaaaaatt 
      tatacctac gatgttgac cacgaaaaa cgacggaaa 
      gagcagctg aagaagctc tacgacagg actcaggaa 
      caaattgaa gaggaacag ttcttgctc ctggagttg 
      aaaaagatc gaagctagg aagaaggag cgcgagaga 
      aagacacaa gacttgcaa aaattgatc agtcaggcg 
      gacagtcag agtgaaact ccccgaaaa acggacaag 
      aaattgccc aagaaaaaa atcgcgaac ccttcaaga 
      ccctcgcgc gttgacaca gcgcatatt ttacaggct 
      gttgagacc gcaggtatc aaattcccc gattacaaa 
      aacagcggc gtttcccta aggtcgcaa cgaatgaaa 
      ctgccggca aatgtgggc cagaaaaaa tcgaaagga 
      atcgaacaa atgttgcaa gaaattggt ttagaatta 
      aatccgatt cctacagaa gaaatctgt caaaatttc 
      aatgagctg aggagtgat atggtcctc ttgatggaa 
      attaaatca gctttgagt acctgcgaa tttgaatta 
      cagtccttg agacatcag tatgaggcg ttaaatccc 
      gggaaaaca ctgacgatc ccgccgcag ttaatctct 
      aatttggat ctcgaaaat aaagctgga acgggggag 
      attatagat gtcgtgggg tctccgagc acccctaca 
      tcttag
    mRNA sequence
    • >TC009994 RA
      tgccaccga catatgaaa atttagcga ttgtgttta 
      tttttgaca gtgacagta aaatgccga ttgttttcg 
      tattacaag aagtgttaa aacctgcta attaagtga 
      aaatgtgta gtctttaat aataaatgt cattatagt 
      ccccgttta cacctcaat ttcaagttt tcttcctac 
      gatcaaaac aagacaatt aaaaatggc cgatgttcg 
      cgatattat ggagctgga gcatccagc gagccaaga 
      ggtgactcg agagtcaat cattggttc agacaagca 
      gaggaagcg gcccccaac tactacaaa aacccagaa 
      aaggcctga gggaatgca ccgtgaagt tttcgcctt 
      gttgtacaa tgataataa ggacgtttc tccgctttt 
      tccgtctga tacaggaca tgggtataa acaaacgaa 
      aattaagtt gggcatgag gaaaccgag aaagtggaa 
      gtgggtgcc gtttacgaa cccggcaag gaccgatgg 
      ggcggtgtt tcatcactg gaggaggcc gtccgacga 
      acctaagga atatccttt tgccaagtt taacaagaa 
      agttgatat ttgtacata cactgatgc cgaatacca 
      gcagcactt aagagttga tggttggac caaggacga 
      aactgacca catgatgca actggcaca gagattcga 
      tctcaggtt tatcctaat ggctgatag atatgacac 
      tgaaaagtt tccgaaaag atcagtgga ggatattaa 
      agaccgtta ctataaaat ttgcggcat catgagcaa 
      attaagagg ggagaaaaa aatttatac ctacgatgt 
      tgaccacga aaaacgacg gaaagagca gctgaagaa 
      gctctacga caggactca ggaacaaat tgaagagga 
      acagttctt gctcctgga gttgaaaaa gatcgaagc 
      taggaagaa ggagcgcga gagaaagac acaagactt 
      gcaaaaatt gatcagtca ggcggacag tcagagtga 
      aactccccg aaaaacgga caagaaatt gcccaagaa 
      aaaaatcgc gaacccttc aagaccctc gcgcgttga 
      cacagcgca tattttaca ggctgttga gaccgcagg 
      tatcaaatt ccccgatta caaaaacag cggcgtttc 
      cctaaggtc gcaacgaat gaaactgcc ggcaaatgt 
      gggccagaa aaaatcgaa aggaatcga acaaatgtt 
      gcaagaaat tggtttaga attaaatcc gattcctac 
      agaagaaat ctgtcaaaa tttcaatga gctgaggag 
      tgatatggt cctcttgat ggaaattaa atcagcttt 
      gagtacctg cgaatttga attacagtc cttgagaca 
      tcagtatga ggcgttaaa tcccgggaa aacactgac 
      gatcccgcc gcagttaat ctctaattt ggatctcga 
      aaataaagc tggaacggg ggagattat agatgtcgt 
      ggggtctcc gagcacccc tacatctta gtataatat 
      aacaaaata atgtaatat taaggtttg aataaacat 
      ttaatttca tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY