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Gene information
General information
TC010963 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3404785..3409637
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC010963
      iBeetle screen iB_10995

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC010963 RA
        MVHYYRLSL KSRQKAPKI VNSKYNSIL NIALKNFRL 
        CKKHKTKKP VQILALLQE IIPKSYFGT TTNLKRFYK 
        VVEKILTQS SFECIHLSV LHKCYDYDA IPWLQNVEP 
        NLRPKLLLK HNLFLLDNI VKPIIAFYY KPIKTLNGH 
        EIKFIRKEE YISFESKVF HKLKKMKYL VEVQDEVKP 
        RGVLNIIPK QDNFRAIVS IFPDSARKP FFKLLTSKI 
        YKVLEEKYK TSGSLYTCW SEFTQKTQG QIYGIKVDI 
        RDAYGNVKI PVLCKLIQS IPTHLLDSE KKNFIVDHI 
        SNQFVAFRR KIYKWNHGL LQGDPLSGC LCELYMAFM 
        DRLYFSNLD KDAFIHRTV DDYFFCSPH PHKVYDFEL 
        LIKGVYQVN PTKTRTNLP THRHPQDEI PYCGKIFNL 
        TTRQVRTLY KLPPNYEIR HKFKLWNFN NQISDDNPA 
        RFLQKAMDF PFICNSFTK FEFNTVFND QRTVFANFY 
        DAMICVAYK FDAAMMALR TSFLVNDFG FIWLVLSST 
        VRAYASRAF KKIVTYKGG KYRKVTFQC LKSIAWRAF 
        LAVLKRRTE IYKGLIDRI KSREKLTMK FHDGEVDAS 
        YFCKLPEKF RFVKINRKA SI
      CDS sequence
      • >TC010963 RA
        atggtccac tactatcgc ctttcgtta aaatcccgc 
        cagaaagct ccaaaaata gtcaactca aaatacaac 
        agcatctta aacattgct ctaaaaaac ttccgacta 
        tgtaaaaag cacaagacc aagaaaccc gtccaaatt 
        ctcgcactt ttacaggaa atcattcca aaatcttac 
        ttcggaaca accacaaac ctcaaacgt ttctacaaa 
        gtcgtcgaa aaaattcta acccaatcg tcattcgag 
        tgcatccat ttatcagtc ctccataaa tgctacgat 
        tatgatgca attccctgg ctacaaaac gtcgaaccg 
        aacctccgc cctaagcta ttgctcaaa cacaattta 
        ttcctcctc gacaacata gtcaaacct ataatcgcc 
        ttttactac aaacccatc aaaacactg aacggacac 
        gaaatcaaa ttcatccgc aaagaggag tacatttca 
        ttcgagagc aaggtgttt cacaaattg aagaaaatg 
        aagtatttg gtcgaggtc caagacgag gtgaaacca 
        cgaggtgtt ttgaacata atcccaaaa caagacaac 
        tttcgcgca atcgtgagc atattccca gattcggcg 
        cgaaaacca ttttttaaa cttttaacg tccaaaatc 
        tacaaagtg ttggaagag aaatacaaa acaagcggg 
        tctctctac acgtgctgg tcggaattc actcaaaaa 
        acacaaggg cagatttat ggcatcaaa gttgatatt 
        agagacgcg tacggaaac gtcaaaatt ccagttctt 
        tgtaaatta attcagagc attccgaca catttgctg 
        gattcggaa aagaaaaat tttattgta gaccacatc 
        agcaaccaa tttgtggct tttaggcga aagatttac 
        aagtggaac cacggattg ctacaaggt gatccctta 
        tcaggctgt ttgtgtgaa ctctacatg gcttttatg 
        gaccgactt tacttttcc aacttggac aaagacgct 
        ttcatccat cgaaccgtc gacgattat ttcttctgc 
        tcacctcac cctcacaaa gtctacgac ttcgaattg 
        ctaatcaaa ggcgtctat caagtaaat cccaccaaa 
        acacgaaca aatttacca acccatcgc cacccacaa 
        gacgaaatt ccctattgc ggaaaaatc ttcaatttg 
        acgacacga caagtgcgt acgctatac aaactgcct 
        ccgaattac gagattcga cacaaattc aaactgtgg 
        aattttaac aatcaaatc agcgacgat aacccggcg 
        agatttctc caaaaagcg atggacttt ccattcatt 
        tgtaacagt ttcacgaag tttgaattc aacacagtt 
        ttcaacgac caaaggacc gtttttgcg aatttctac 
        gatgctatg atttgtgtc gcttataag tttgatgca 
        gctatgatg gcattaaga actagtttt ttggtcaat 
        gattttggt tttatttgg ttagttttg agttcgaca 
        gttagagca tatgcctcg agggcgttt aaaaaaatc 
        gtaacttat aaaggtggc aaatatcgg aaagtaaca 
        tttcagtgt ttgaaaagt atcgcttgg cgggctttt 
        cttgctgtt cttaagcga agaactgaa atttataaa 
        gggttaatt gacagaatt aaaagccgg gaaaagttg 
        actatgaag tttcatgat ggggaggtg gatgcgagt 
        tatttttgt aaattgccg gaaaagttt agatttgta 
        aaaatcaac agaaaggcc tcaatttaa
      mRNA sequence
      • >TC010963 RA
        cacgctatt gactataca acaccttga taagtttgt 
        ttacaaagt gtaaaacca taatgtaaa acattggtg 
        aattatgtt taaatgaaa tgaaataat ggtccacta 
        ctatcgcct ttcgttaaa atcccgcca gaaagctcc 
        aaaaatagt caactcaaa atacaacag catcttaaa 
        cattgctct aaaaaactt ccgactatg taaaaagca 
        caagaccaa gaaacccgt ccaaattct cgcactttt 
        acaggaaat cattccaaa atcttactt cggaacaac 
        cacaaacct caaacgttt ctacaaagt cgtcgaaaa 
        aattctaac ccaatcgtc attcgagtg catccattt 
        atcagtcct ccataaatg ctacgatta tgatgcaat 
        tccctggct acaaaacgt cgaaccgaa cctccgccc 
        taagctatt gctcaaaca caatttatt cctcctcga 
        caacatagt caaacctat aatcgcctt ttactacaa 
        acccatcaa aacactgaa cggacacga aatcaaatt 
        catccgcaa agaggagta catttcatt cgagagcaa 
        ggtgtttca caaattgaa gaaaatgaa gtatttggt 
        cgaggtcca agacgaggt gaaaccacg aggtgtttt 
        gaacataat cccaaaaca agacaactt tcgcgcaat 
        cgtgagcat attcccaga ttcggcgcg aaaaccatt 
        ttttaaact tttaacgtc caaaatcta caaagtgtt 
        ggaagagaa atacaaaac aagcgggtc tctctacac 
        gtgctggtc ggaattcac tcaaaaaac acaagggca 
        gatttatgg catcaaagt tgatattag agacgcgta 
        cggaaacgt caaaattcc agttctttg taaattaat 
        tcagagcat tccgacaca tttgctgga ttcggaaaa 
        gaaaaattt tattgtaga ccacatcag caaccaatt 
        tgtggcttt taggcgaaa gatttacaa gtggaacca 
        cggattgct acaaggtga tcccttatc aggctgttt 
        gtgtgaact ctacatggc ttttatgga ccgacttta 
        cttttccaa cttggacaa agacgcttt catccatcg 
        aaccgtcga cgattattt cttctgctc acctcaccc 
        tcacaaagt ctacgactt cgaattgct aatcaaagg 
        cgtctatca agtaaatcc caccaaaac acgaacaaa 
        tttaccaac ccatcgcca cccacaaga cgaaattcc 
        ctattgcgg aaaaatctt caatttgac gacacgaca 
        agtgcgtac gctatacaa actgcctcc gaattacga 
        gattcgaca caaattcaa actgtggaa ttttaacaa 
        tcaaatcag cgacgataa cccggcgag atttctcca 
        aaaagcgat ggactttcc attcatttg taacagttt 
        cacgaagtt tgaattcaa cacagtttt caacgacca 
        aaggaccgt ttttgcgaa tttctacga tgctatgat 
        ttgtgtcgc ttataagtt tgatgcagc tatgatggc 
        attaagaac tagtttttt ggtcaatga ttttggttt 
        tatttggtt agttttgag ttcgacagt tagagcata 
        tgcctcgag ggcgtttaa aaaaatcgt aacttataa 
        aggtggcaa atatcggaa agtaacatt tcagtgttt 
        gaaaagtat cgcttggcg ggcttttct tgctgttct 
        taagcgaag aactgaaat ttataaagg gttaattga 
        cagaattaa aagccggga aaagttgac tatgaagtt 
        tcatgatgg ggaggtgga tgcgagtta tttttgtaa 
        attgccgga aaagtttag atttgtaaa aatcaacag 
        aaaggcctc aatttaaga ttgtcaata agtgttatg 
        ttttttttt ataaaattt ctatctata tattataat 
        aaataaaaa gtacagtgt gtt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY