Status
Please wait ...
Gene information
General information
TC032288
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003239
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 196519..198123
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032288
    iBeetle screen iB_10993

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 3
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032288 RA
      MVVKKICCV GAGYVGGPT CSVIALKCP EIHVTVVDL 
      SKSRIDQWN SDKLPIYEP GLDEIVKQC RGRNLFFST 
      DIETAILEA DLIFISVNT PTKTIGNGK GRAPDLKYV 
      EGAARMIAE IAQSDKIVV EKSTVPVRA AESILKILS 
      ANQKPGVSY QILSNPEFL AEGTAINDL INADRVLIG 
      GEESPKGKA AIEELCGIY EHWIPREKI FTTNTWSSE 
      LSKLAANAM LAQRISSIN SLSAVCEAT GADVSEVAT 
      AVGLDSRIG SKFLQASIG RCRFLD
    CDS sequence
    • >TC032288 RA
      atggttgtt aaaaaaatt tgttgtgtg ggggctggt 
      tacgtcggt ggcccaacg tgtagtgtc atagctttg 
      aaatgtcca gaaattcat gtaacagtg gtagattta 
      tcaaaatca cgaattgat caatggaat tcggacaaa 
      cttcctatt tacgagccg ggtttggac gaaattgtt 
      aaacaatgc agagggagg aatctattt ttctccacc 
      gacattgaa actgcaatt ttagaagct gacttaata 
      tttatttca gtgaatacg ccgacaaag accattgga 
      aatgggaag ggcagggcc cctgacttg aaatacgtc 
      gaaggcgcc gctcgaatg attgccgaa atcgcacaa 
      agcgataaa atcgtcgtc gaaaaaagc acagttccg 
      gtccgagcc gctgaaagc attctaaaa attttaagt 
      gccaaccaa aaaccggga gtttcttat cagattctc 
      tcaaatccg gaattttta gccgaaggc acagctatt 
      aacgatttg attaatgcc gaccgtgtt ctaatcggt 
      ggtgaagaa agtcctaaa ggaaaagca gcaattgaa 
      gaattgtgc ggtatttac gaacattgg attccacga 
      gaaaaaatt ttcacaaca aatacttgg tcatcggaa 
      ttatccaaa cttgctgcg aatgcaatg ttggctcaa 
      cgaatctca agtattaat tcattgtcg gctgtttgt 
      gaagcaacc ggtgctgat gtttccgaa gttgcgact 
      gctgttgga ttagattcg aggattgga tcgaaattt 
      ttgcaagct tcaataggt agatgtcgc tttcttgat 
      taa
    mRNA sequence
    • >TC032288 RA
      cagatgacg tttcgtcag acgtcattt ccgtttttc 
      tcattattt aactttata tttccgagg ttcaaatta 
      aatcaaaat tcagtttta gaatgtgtc tgagttaaa 
      agcgattca cctcaccta ttatcacac taggtaggg 
      ataaaaagc gacgaatca aaaggtttc ggtgtgttt 
      gcgtgcggg aggtagaag aggttattg taaaccaac 
      taaaaaatt tctttagat ttgtgttct cagaattca 
      aacttgtgg acgtggttc tttgaaacg acaatggtt 
      gttaaaaaa atttgttgt gtgggggct ggttacgtc 
      ggtggccca acgtgtagt gtcatagct ttgaaatgt 
      ccagaaatt catgtaaca gtggtagat ttatcaaaa 
      tcacgaatt gatcaatgg aattcggac aaacttcct 
      atttacgag ccgggtttg gacgaaatt gttaaacaa 
      tgcagaggg aggaatcta tttttctcc accgacatt 
      gaaactgca attttagaa gctgactta atatttatt 
      tcagtgaat acgccgaca aagaccatt ggaaatggg 
      aagggcagg gcccctgac ttgaaatac gtcgaaggc 
      gccgctcga atgattgcc gaaatcgca caaagcgat 
      aaaatcgtc gtcgaaaaa agcacagtt ccggtccga 
      gccgctgaa agcattcta aaaatttta agtgccaac 
      caaaaaccg ggagtttct tatcagatt ctctcaaat 
      ccggaattt ttagccgaa ggcacagct attaacgat 
      ttgattaat gccgaccgt gttctaatc ggtggtgaa 
      gaaagtcct aaaggaaaa gcagcaatt gaagaattg 
      tgcggtatt tacgaacat tggattcca cgagaaaaa 
      attttcaca acaaatact tggtcatcg gaattatcc 
      aaacttgct gcgaatgca atgttggct caacgaatc 
      tcaagtatt aattcattg tcggctgtt tgtgaagca 
      accggtgct gatgtttcc gaagttgcg actgctgtt 
      ggattagat tcgaggatt ggatcgaaa tttttgcaa 
      gcttcaata ggtagatgt cgctttctt gattaaatt 
      tatatacac agtgtctca gta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY