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Gene information
General information
TC031696 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451215.1: 54015..55829
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031696
    iBeetle screen iB_10990

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031696 RA
      MELSERTLL LDHTASTKN CHNKLSVIF AIVCIVDVF 
      GVFPVTTLP KAIIDCGIY GGIILCIVC SIQIYTAIL 
      LGKSWIIAE KIDVSVETK NRYPYSALA EITYGTTFS 
      NFVSFLVNV TIFGGGIPN LIVASQNIE LLGLRLSSD 
      TFEVSFCYW IIIIGMILC PVLWLGSPK DMKLLCTIS 
      VANVVIVFL LVTECLIFS SRTPTTTDD SFQTEQSFW 
      ELMFICYGI ISFQFDIHP SILTIQVDM REKSKLNSA 
      VLGGFAISL GMFSIVTAL AASKYGLSV KPSLLETLP 
      TTIPLHFAA LFVALQLCL SSAVSNSAL YQYMEDCMS 
      ISPGKKSLL L
    CDS sequence
    • >TC031696 RA
      atggaatta tccgaacga actttgtta ttagaccat 
      acggcatca actaaaaat tgccacaat aaactgtct 
      gtgattttt gcaatagtt tgcattgtt gatgttttt 
      ggggtcttt ccggtcaca acattacca aaagcaatc 
      atcgattgt ggtatttac ggcggaata atattatgc 
      attgtatgt tccattcaa atttacaca gcgatttta 
      ttgggcaaa agttggata atcgcagaa aaaatagac 
      gtgtcagtt gaaaccaag aacagatat ccgtattca 
      gcattagct gaaatcacc tacggcacc actttctca 
      aattttgtc tcatttctt gtaaatgtc accattttt 
      gggggcgga atacccaat ttaatcgtt gcttctcaa 
      aatatcgaa cttttgggc ttgagacta agtagtgac 
      acgtttgaa gtctcattt tgttactgg attattata 
      attggaatg attttgtgc ccagttttg tggctaggg 
      agtcccaaa gatatgaaa ttactttgc accatttct 
      gtggctaac gttgttata gtttttctt ctcgtgact 
      gaatgcctg attttcagt agtcgaaca ccgactacg 
      actgatgat agtttccaa acagagcag tctttctgg 
      gaattaatg tttatctgt tacgggatt atatcgttt 
      caatttgat atccaccca agtatattg acaattcaa 
      gtggatatg agggaaaaa tccaaactc aatagtgcc 
      gtacttggc ggttttgct atttcttta gggatgttt 
      tctattgtt accgctctc gccgcaagt aagtacggc 
      ttatcagtt aaaccaagt cttttagag acccttcca 
      acaacaatt cctttacat tttgctgcc ctttttgtc 
      gcacttcaa ctttgttta tcttcagca gttagtaac 
      agtgccctt tatcaatat atggaggac tgtatgagc 
      atttcacca ggcaaaaaa tcactttta ttgtga
    mRNA sequence
    • >TC031696 RA
      attgggtta taaacagtc cagacagtt tgcgagatc 
      aataccaac cacttatta tgttagaaa tgtgatatg 
      tcctttata ccgagttgt aactgaaac ctttccacc 
      taaaaccca tagagcgta ctaatcgta tccagtgcc 
      aacatggaa ttatccgaa cgaactttg ttattagac 
      catacggca tcaactaaa aattgccac aataaactg 
      tctgtgatt tttgcaata gtttgcatt gttgatgtt 
      tttggggtc tttccggtc acaacatta ccaaaagca 
      atcatcgat tgtggtatt tacggcgga ataatatta 
      tgcattgta tgttccatt caaatttac acagcgatt 
      ttattgggc aaaagttgg ataatcgca gaaaaaata 
      gacgtgtca gttgaaacc aagaacaga tatccgtat 
      tcagcatta gctgaaatc acctacggc accactttc 
      tcaaatttt gtctcattt cttgtaaat gtcaccatt 
      tttgggggc ggaataccc aatttaatc gttgcttct 
      caaaatatc gaacttttg ggcttgaga ctaagtagt 
      gacacgttt gaagtctca ttttgttac tggattatt 
      ataattgga atgattttg tgcccagtt ttgtggcta 
      gggagtccc aaagatatg aaattactt tgcaccatt 
      tctgtggct aacgttgtt atagttttt cttctcgtg 
      actgaatgc ctgattttc agtagtcga acaccgact 
      acgactgat gatagtttc caaacagag cagtctttc 
      tgggaatta atgtttatc tgttacggg attatatcg 
      tttcaattt gatatccac ccaagtata ttgacaatt 
      caagtggat atgagggaa aaatccaaa ctcaatagt 
      gccgtactt ggcggtttt gctatttct ttagggatg 
      ttttctatt gttaccgct ctcgccgca agtaagtac 
      ggcttatca gttaaacca agtctttta gagaccctt 
      ccaacaaca attccttta cattttgct gcccttttt 
      gtcgcactt caactttgt ttatcttca gcagttagt 
      aacagtgcc ctttatcaa tatatggag gactgtatg 
      agcatttca ccaggcaaa aaatcactt ttattgtga 
      cagtatgaa aataaatca atttacagc gttcaatca 
      tcgtcgttg cgttttgag aacaacatt aacattttt 
      agctgtctt gatagccga atctgtgcc tcgctttga 
      ccttgttat gtcacttat cggagggac tttaactgg 
      cccattgat tttcatatt tcctccttt attttactt 
      gaaaatgtt atcaatgga aaataagca aatgaagca 
      aatggcaat ggagacttt cactagtgt agtgtacag 
      tcgtaatga agaagaaaa aattttgac aaataccga 
      tgagtgtca gttcgaaat aactaaaag tattttagg 
      tgcaaaact tgatattgt ttgttgtgt aacaattat 
      aactttaag tgctgtctg cactgtaat tacgactta 
      cgtgaacat aaggaatgc cattgcttt tgtcaattt 
      tacgcaacc ctgtatttt taacgttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY