Status
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Gene information
General information
TC010230 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 1236187..1339804
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC010230
      iBeetle screen iB_10989

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC010230 RA
        MLHRSTSSR RRRASRSAA SSPQTRPSR SAGTSPGRR 
        ASIAVSPVD SERASRNTL APEINRSPR HSIIPDISL 
        DSESLRSPR HSLAPEVLS PRNSLVPDT AGLRSPRHS 
        LVPNDTYSR SPRNSLVPD SALSPRNSL VPDSFNRSP 
        RNSLVPEVS KSGRNSLVP DSNRSPRHS LVPDSNTYG 
        SRANLAPDF NRSPRNSLI PDSSRTPRA SLTPSESTT 
        WHKDEETGS RSPRHSIQV PDSSRSPRG SIAPESLCV 
        RTSPRGSIA GEYPCDRSP RGSIGPESG GENRSPRGS 
        IARSTLNAE DCGRSPRGS LTLTFQEPP ANERRSSAD 
        NPSGSRSGG RSTSPYRSS SRGAVNAAY SGNSGFSES 
        GSRRASSSV SQVSGDEQR RLCSSDQKY GGGEQGLGL 
        GISLTTYGS VAYQLKDAN LEASGTCDF VCKALSIVN 
        KTVVVTIVL VCLSTLPLL MLIMGIQFL KDCPREPYI 
        PIYMVVGGS VGCVKMGLT LYNQLHTRR MDASSTANA 
        ASIGSRVAS IALTGFLII WFSFGNYWV LRIKWPDYA 
        PTLFEPNHW CHRTFEKRR EVS
      • >TC010230 RB
        MLHRSTSSR RRRASRSAA SSPQTRPSR SAGTSPGRR 
        ASIAVSPVD SERASRNTL APEINRSPR HSIIPDISL 
        DSESLRSPR HSLAPEVLS PRNSLVPDT AGLRSPRHS 
        LVPNDTYSR SPRNSLVPD SALSPRNSL VPDSFNRSP 
        RNSLVPEVS KSGRNSLVP DSNRSPRHS LVPDSNTYG 
        SRANLAPDF NRSPRNSLI PDSSRTPRA SLTPSESTT 
        WHKDEETGS RSPRHSIQV PDSSRSPRG SIAPESLCV 
        RTSPRGSIA GEYPCDRSP RGSIGPESG GENRSPRGS 
        IARSTLNAE DCGRSPRGS LTLTFQEPP ANERRSSAD 
        NPSGSRSGG RSTSPYRSS SRGAVNAAY SGNSGFSES 
        GSRRASSSV SQVGLPQVS GDEQRRLCS SDQKYGGGE 
        QGLGLGISL TTYGSVAYQ LKDANLEAS GTCDFVCKA 
        LSIVNKTVV VTIVLVCLS TLPLLMLIM GIQFLKDCP 
        REPYIPIYM VVGGSVGCV KMGLTLYNQ LHTRRMDAS 
        STANAASIG SRVASIALT GFLIIWFSF GNYWVLRIK 
        WPDYAPTLF EPNHWCHRT LYVFALVHL CVVYSVMGC 
        VVVLMLVLA GCQMFGCPW LGPARYK
      • >TC010230 RC
        MLHRSTSSR RRRASRSAA SSPQTRPSR SAGTSPGRR 
        ASIAVSPVD SERASRNTL APEINRSPR HSIIPDISL 
        DSESLRSPR HSLAPEVLS PRNSLVPDT AGLRSPRHS 
        LVPNDTYSR SPRNSLVPD SALSPRNSL VPDSFNRSP 
        RNSLVPEVS KSGRNSLVP DSNRSPRHS LVPDSNTYG 
        SRANLAPDF NRSPRNSLI PDSSRTPRA SLTPSESTT 
        WHKDEETGS RSPRHSIQV PDSSRSPRG SIAPESLCV 
        RTSPRGSIA GEYPCDRSP RGSIGPESG GENRSPRGS 
        IARSTLNAE DCGRSPRGS LTLTFQEPP ANERRSSAD 
        NPSGSRSGG RSTSPYRSS SRGAVNAAY SGNSGFSES 
        GSRRASSSV SQVSGDEQR RLCSSDQKY GGGEQGLGL 
        GISLTTYGS VAYQLKDAN LEASGTCDF VCKALSIVN 
        KTVVVTIVL VCLSTLPLL MLIMGIQFL KDCPREPYI 
        PIYMVVGGS VGCVKMGLT LYNQLHTRR MDASSTANA 
        ASIGSRVAS IALTGFLII WFSFGNYWV LRIKWPDYA 
        PTLFEPNHW CHRTLYVFA LVHLCVVYS VMGCVVVLM 
        LVLAGCQMF GCPWLGPAR YK
      CDS sequence
      • >TC010230 RA
        atgctccac cgaagcacc tcgtctcgt cgcagacga 
        gcatcacga agtgcagcc tcatctcct caaacccgt 
        ccttctcgc tcggctgga acaagcccc ggtcgtcga 
        gcaagcatt gcagtttca ccagtggac agtgagcga 
        gcctccaga aacaccctc gcacctgaa ataaaccgc 
        agtccacga cacagcatc attccagac atttcccta 
        gattcggaa agtcttcga agccctcga cacagtctc 
        gccccggaa gtcctaagt ccaagaaat agtctagtt 
        ccggacact gcaggcttg agaagtcca agacatagt 
        ctagtgcca aatgacact tactcgcga agtcctcga 
        aacagttta gtgcccgat tctgcgtta agtcctcga 
        aatagttta gtgccggat agttttaat cgaagtccg 
        cgaaatagt ctcgttccg gaagttagt aaaagtggc 
        cgaaatagt ctagtcccg gattcgaat cgtagtcct 
        cgacacagt ttagtacct gatagtaat acctatggt 
        tcgagggca aatttagca ccggatttt aaccgaagt 
        cccagaaat agtcttatt cctgattca agcagaacc 
        ccaagagct agtctcaca ccgtccgag tccacaaca 
        tggcacaaa gatgaagag acggggagc agaagtcca 
        cgtcatagt atacaggtt ccagatagc agtcgaagt 
        cctagagga agtatagct cctgaaagt ctctgcgtt 
        aggacgagt ccgcgtgga agcatagct ggggaatat 
        ccatgcgat cgaagccct cgagggagt attgggcct 
        gaaagtggt ggtgagaac aggagtccg agaggaagc 
        atcgccaga agtacgctg aatgcggag gattgtggt 
        cgaagtccc agaggaagt ctgacgttg actttccag 
        gagccgcct gctaatgaa cgacgctcg agtgcagac 
        aatccttcc ggatcaagg agcggaggt cgtagcaca 
        tctccttat agatcttcc tcaagaggc gcagtcaat 
        gcggcttac agcgggaat tctggattc tcggagagt 
        ggatccaga cgtgccagc agctctgtt agccaggtg 
        tccggggac gagcagcgc cgcctttgc tcctccgac 
        cagaaatac gggggcggc gaacaggga ctggggctc 
        ggtatctca ctcactact tatggatcg gtggcctat 
        caattgaaa gacgccaat ttagaagcg tctggaaca 
        tgcgatttt gtctgcaaa gcattgagc atcgtcaat 
        aaaacagtg gttgttacg atcgttctg gtgtgctta 
        tccactcta cctctacta atgcttatc atgggtatt 
        caattcctg aaagactgc cccagagag ccctacatt 
        cccatctac atggtggtt gggggcagt gtgggctgc 
        gtgaaaatg gggctcacc ctctacaac cagctgcac 
        acccgtcgg atggacgct tccagcaca gctaacgcc 
        gcttccatc ggctcgaga gtcgcttcg atcgccctc 
        accggattc ctgatcatc tggttcagt tttggcaac 
        tactgggtc ttgaggatc aaatggccg gactatgct 
        cccacatta ttcgagccg aaccattgg tgccatcgc 
        actttcgag aagcgaaga gaagtatct taa
      • >TC010230 RB
        atgctccac cgaagcacc tcgtctcgt cgcagacga 
        gcatcacga agtgcagcc tcatctcct caaacccgt 
        ccttctcgc tcggctgga acaagcccc ggtcgtcga 
        gcaagcatt gcagtttca ccagtggac agtgagcga 
        gcctccaga aacaccctc gcacctgaa ataaaccgc 
        agtccacga cacagcatc attccagac atttcccta 
        gattcggaa agtcttcga agccctcga cacagtctc 
        gccccggaa gtcctaagt ccaagaaat agtctagtt 
        ccggacact gcaggcttg agaagtcca agacatagt 
        ctagtgcca aatgacact tactcgcga agtcctcga 
        aacagttta gtgcccgat tctgcgtta agtcctcga 
        aatagttta gtgccggat agttttaat cgaagtccg 
        cgaaatagt ctcgttccg gaagttagt aaaagtggc 
        cgaaatagt ctagtcccg gattcgaat cgtagtcct 
        cgacacagt ttagtacct gatagtaat acctatggt 
        tcgagggca aatttagca ccggatttt aaccgaagt 
        cccagaaat agtcttatt cctgattca agcagaacc 
        ccaagagct agtctcaca ccgtccgag tccacaaca 
        tggcacaaa gatgaagag acggggagc agaagtcca 
        cgtcatagt atacaggtt ccagatagc agtcgaagt 
        cctagagga agtatagct cctgaaagt ctctgcgtt 
        aggacgagt ccgcgtgga agcatagct ggggaatat 
        ccatgcgat cgaagccct cgagggagt attgggcct 
        gaaagtggt ggtgagaac aggagtccg agaggaagc 
        atcgccaga agtacgctg aatgcggag gattgtggt 
        cgaagtccc agaggaagt ctgacgttg actttccag 
        gagccgcct gctaatgaa cgacgctcg agtgcagac 
        aatccttcc ggatcaagg agcggaggt cgtagcaca 
        tctccttat agatcttcc tcaagaggc gcagtcaat 
        gcggcttac agcgggaat tctggattc tcggagagt 
        ggatccaga cgtgccagc agctctgtt agccaggtg 
        ggcctgccg caggtgtcc ggggacgag cagcgccgc 
        ctttgctcc tccgaccag aaatacggg ggcggcgaa 
        cagggactg gggctcggt atctcactc actacttat 
        ggatcggtg gcctatcaa ttgaaagac gccaattta 
        gaagcgtct ggaacatgc gattttgtc tgcaaagca 
        ttgagcatc gtcaataaa acagtggtt gttacgatc 
        gttctggtg tgcttatcc actctacct ctactaatg 
        cttatcatg ggtattcaa ttcctgaaa gactgcccc 
        agagagccc tacattccc atctacatg gtggttggg 
        ggcagtgtg ggctgcgtg aaaatgggg ctcaccctc 
        tacaaccag ctgcacacc cgtcggatg gacgcttcc 
        agcacagct aacgccgct tccatcggc tcgagagtc 
        gcttcgatc gccctcacc ggattcctg atcatctgg 
        ttcagtttt ggcaactac tgggtcttg aggatcaaa 
        tggccggac tatgctccc acattattc gagccgaac 
        cattggtgc catcgcact ttgtacgtg tttgcgttg 
        gttcatctc tgtgtggtg tattcggtg atggggtgt 
        gtggtggtg ttaatgttg gtgttggct ggatgtcaa 
        atgtttggg tgcccttgg cttggaccg gcaaggtac 
        aaatag
      • >TC010230 RC
        atgctccac cgaagcacc tcgtctcgt cgcagacga 
        gcatcacga agtgcagcc tcatctcct caaacccgt 
        ccttctcgc tcggctgga acaagcccc ggtcgtcga 
        gcaagcatt gcagtttca ccagtggac agtgagcga 
        gcctccaga aacaccctc gcacctgaa ataaaccgc 
        agtccacga cacagcatc attccagac atttcccta 
        gattcggaa agtcttcga agccctcga cacagtctc 
        gccccggaa gtcctaagt ccaagaaat agtctagtt 
        ccggacact gcaggcttg agaagtcca agacatagt 
        ctagtgcca aatgacact tactcgcga agtcctcga 
        aacagttta gtgcccgat tctgcgtta agtcctcga 
        aatagttta gtgccggat agttttaat cgaagtccg 
        cgaaatagt ctcgttccg gaagttagt aaaagtggc 
        cgaaatagt ctagtcccg gattcgaat cgtagtcct 
        cgacacagt ttagtacct gatagtaat acctatggt 
        tcgagggca aatttagca ccggatttt aaccgaagt 
        cccagaaat agtcttatt cctgattca agcagaacc 
        ccaagagct agtctcaca ccgtccgag tccacaaca 
        tggcacaaa gatgaagag acggggagc agaagtcca 
        cgtcatagt atacaggtt ccagatagc agtcgaagt 
        cctagagga agtatagct cctgaaagt ctctgcgtt 
        aggacgagt ccgcgtgga agcatagct ggggaatat 
        ccatgcgat cgaagccct cgagggagt attgggcct 
        gaaagtggt ggtgagaac aggagtccg agaggaagc 
        atcgccaga agtacgctg aatgcggag gattgtggt 
        cgaagtccc agaggaagt ctgacgttg actttccag 
        gagccgcct gctaatgaa cgacgctcg agtgcagac 
        aatccttcc ggatcaagg agcggaggt cgtagcaca 
        tctccttat agatcttcc tcaagaggc gcagtcaat 
        gcggcttac agcgggaat tctggattc tcggagagt 
        ggatccaga cgtgccagc agctctgtt agccaggtg 
        tccggggac gagcagcgc cgcctttgc tcctccgac 
        cagaaatac gggggcggc gaacaggga ctggggctc 
        ggtatctca ctcactact tatggatcg gtggcctat 
        caattgaaa gacgccaat ttagaagcg tctggaaca 
        tgcgatttt gtctgcaaa gcattgagc atcgtcaat 
        aaaacagtg gttgttacg atcgttctg gtgtgctta 
        tccactcta cctctacta atgcttatc atgggtatt 
        caattcctg aaagactgc cccagagag ccctacatt 
        cccatctac atggtggtt gggggcagt gtgggctgc 
        gtgaaaatg gggctcacc ctctacaac cagctgcac 
        acccgtcgg atggacgct tccagcaca gctaacgcc 
        gcttccatc ggctcgaga gtcgcttcg atcgccctc 
        accggattc ctgatcatc tggttcagt tttggcaac 
        tactgggtc ttgaggatc aaatggccg gactatgct 
        cccacatta ttcgagccg aaccattgg tgccatcgc 
        actttgtac gtgtttgcg ttggttcat ctctgtgtg 
        gtgtattcg gtgatgggg tgtgtggtg gtgttaatg 
        ttggtgttg gctggatgt caaatgttt gggtgccct 
        tggcttgga ccggcaagg tacaaatag
      mRNA sequence
      • >TC010230 RA
        gctgtgtaa gaaaattct gcgagattc ttagacgaa 
        atgctccac cgaagcacc tcgtctcgt cgcagacga 
        gcatcacga agtgcagcc tcatctcct caaacccgt 
        ccttctcgc tcggctgga acaagcccc ggtcgtcga 
        gcaagcatt gcagtttca ccagtggac agtgagcga 
        gcctccaga aacaccctc gcacctgaa ataaaccgc 
        agtccacga cacagcatc attccagac atttcccta 
        gattcggaa agtcttcga agccctcga cacagtctc 
        gccccggaa gtcctaagt ccaagaaat agtctagtt 
        ccggacact gcaggcttg agaagtcca agacatagt 
        ctagtgcca aatgacact tactcgcga agtcctcga 
        aacagttta gtgcccgat tctgcgtta agtcctcga 
        aatagttta gtgccggat agttttaat cgaagtccg 
        cgaaatagt ctcgttccg gaagttagt aaaagtggc 
        cgaaatagt ctagtcccg gattcgaat cgtagtcct 
        cgacacagt ttagtacct gatagtaat acctatggt 
        tcgagggca aatttagca ccggatttt aaccgaagt 
        cccagaaat agtcttatt cctgattca agcagaacc 
        ccaagagct agtctcaca ccgtccgag tccacaaca 
        tggcacaaa gatgaagag acggggagc agaagtcca 
        cgtcatagt atacaggtt ccagatagc agtcgaagt 
        cctagagga agtatagct cctgaaagt ctctgcgtt 
        aggacgagt ccgcgtgga agcatagct ggggaatat 
        ccatgcgat cgaagccct cgagggagt attgggcct 
        gaaagtggt ggtgagaac aggagtccg agaggaagc 
        atcgccaga agtacgctg aatgcggag gattgtggt 
        cgaagtccc agaggaagt ctgacgttg actttccag 
        gagccgcct gctaatgaa cgacgctcg agtgcagac 
        aatccttcc ggatcaagg agcggaggt cgtagcaca 
        tctccttat agatcttcc tcaagaggc gcagtcaat 
        gcggcttac agcgggaat tctggattc tcggagagt 
        ggatccaga cgtgccagc agctctgtt agccaggtg 
        tccggggac gagcagcgc cgcctttgc tcctccgac 
        cagaaatac gggggcggc gaacaggga ctggggctc 
        ggtatctca ctcactact tatggatcg gtggcctat 
        caattgaaa gacgccaat ttagaagcg tctggaaca 
        tgcgatttt gtctgcaaa gcattgagc atcgtcaat 
        aaaacagtg gttgttacg atcgttctg gtgtgctta 
        tccactcta cctctacta atgcttatc atgggtatt 
        caattcctg aaagactgc cccagagag ccctacatt 
        cccatctac atggtggtt gggggcagt gtgggctgc 
        gtgaaaatg gggctcacc ctctacaac cagctgcac 
        acccgtcgg atggacgct tccagcaca gctaacgcc 
        gcttccatc ggctcgaga gtcgcttcg atcgccctc 
        accggattc ctgatcatc tggttcagt tttggcaac 
        tactgggtc ttgaggatc aaatggccg gactatgct 
        cccacatta ttcgagccg aaccattgg tgccatcgc 
        actttcgag aagcgaaga gaagtatct taaactgtg 
        cagctttat taaataaac attaaacta ccaacaagt 
        taaaacttt gtacagtta gataagtga aacgttggg 
        attgtttcc tcagacact cctgctatg tgagtttag 
        acatgagtt acaaatgtt ttctgcgat aaagtttcc 
        tcaaccatt gaaaaaata aactctttt tttgcaatt 
        tttgcttac ttattaatt gcacgagtg tttgaacta 
        gacactcat ggcttgctt gaaaggatt tttctccat 
        aaaagtcca atttgcttt aatacaagg gttcgcgaa 
        acgggttaa tgttatgct atgtcaaca tttacggtc 
        caatgacag cttatttcc gggaacaat tacaaacat 
        tcctcacat gttcattac cttgtatat gaaggcttt 
        ctcttaact tttgctatc gtacgcctg gagcatttc 
        tggacgtat tttgatatg agatttttt aataaatgg 
        acaaggaag aggcctttg aacaagttt tcttattat 
        gtggattct atttacaac tgcataatc aacagggtt 
        gggttggga acaccaaga acttaaaaa ccgtagatc 
        cattttcat gctttttgt aacatttta ggcttaagc 
        gctatttgt ataattaat tattaaatg gatgtgtat 
        gctgtgcaa gatttcagt ttgtatttt ataaataag 
        ccgtcgttg tagacgacg gaatcgcgg tccaggctt 
        taaaataac gagatatta aactagaaa tatcaaaga 
        cagattctt ttacaacta acagtggga ttttccaaa 
        aaaaaaaca agaacacaa ggaaattgt gttttcctt 
        tatcaacac tagttgtaa acgatctgt tctgctttg 
        tacataggg tcatataat aaacaatct gtatagac
      • >TC010230 RB
        gctgtgtaa gaaaattct gcgagattc ttagacgaa 
        atgctccac cgaagcacc tcgtctcgt cgcagacga 
        gcatcacga agtgcagcc tcatctcct caaacccgt 
        ccttctcgc tcggctgga acaagcccc ggtcgtcga 
        gcaagcatt gcagtttca ccagtggac agtgagcga 
        gcctccaga aacaccctc gcacctgaa ataaaccgc 
        agtccacga cacagcatc attccagac atttcccta 
        gattcggaa agtcttcga agccctcga cacagtctc 
        gccccggaa gtcctaagt ccaagaaat agtctagtt 
        ccggacact gcaggcttg agaagtcca agacatagt 
        ctagtgcca aatgacact tactcgcga agtcctcga 
        aacagttta gtgcccgat tctgcgtta agtcctcga 
        aatagttta gtgccggat agttttaat cgaagtccg 
        cgaaatagt ctcgttccg gaagttagt aaaagtggc 
        cgaaatagt ctagtcccg gattcgaat cgtagtcct 
        cgacacagt ttagtacct gatagtaat acctatggt 
        tcgagggca aatttagca ccggatttt aaccgaagt 
        cccagaaat agtcttatt cctgattca agcagaacc 
        ccaagagct agtctcaca ccgtccgag tccacaaca 
        tggcacaaa gatgaagag acggggagc agaagtcca 
        cgtcatagt atacaggtt ccagatagc agtcgaagt 
        cctagagga agtatagct cctgaaagt ctctgcgtt 
        aggacgagt ccgcgtgga agcatagct ggggaatat 
        ccatgcgat cgaagccct cgagggagt attgggcct 
        gaaagtggt ggtgagaac aggagtccg agaggaagc 
        atcgccaga agtacgctg aatgcggag gattgtggt 
        cgaagtccc agaggaagt ctgacgttg actttccag 
        gagccgcct gctaatgaa cgacgctcg agtgcagac 
        aatccttcc ggatcaagg agcggaggt cgtagcaca 
        tctccttat agatcttcc tcaagaggc gcagtcaat 
        gcggcttac agcgggaat tctggattc tcggagagt 
        ggatccaga cgtgccagc agctctgtt agccaggtg 
        ggcctgccg caggtgtcc ggggacgag cagcgccgc 
        ctttgctcc tccgaccag aaatacggg ggcggcgaa 
        cagggactg gggctcggt atctcactc actacttat 
        ggatcggtg gcctatcaa ttgaaagac gccaattta 
        gaagcgtct ggaacatgc gattttgtc tgcaaagca 
        ttgagcatc gtcaataaa acagtggtt gttacgatc 
        gttctggtg tgcttatcc actctacct ctactaatg 
        cttatcatg ggtattcaa ttcctgaaa gactgcccc 
        agagagccc tacattccc atctacatg gtggttggg 
        ggcagtgtg ggctgcgtg aaaatgggg ctcaccctc 
        tacaaccag ctgcacacc cgtcggatg gacgcttcc 
        agcacagct aacgccgct tccatcggc tcgagagtc 
        gcttcgatc gccctcacc ggattcctg atcatctgg 
        ttcagtttt ggcaactac tgggtcttg aggatcaaa 
        tggccggac tatgctccc acattattc gagccgaac 
        cattggtgc catcgcact ttgtacgtg tttgcgttg 
        gttcatctc tgtgtggtg tattcggtg atggggtgt 
        gtggtggtg ttaatgttg gtgttggct ggatgtcaa 
        atgtttggg tgcccttgg cttggaccg gcaaggtac 
        aaatagcac aatccgcct gattacttc gaactgtgg 
        gacatgccc aggacttac cactgactt gctcaaggt 
        ttgagtttt atcgcttcc attctaaca attacgatc 
        tcttcttga ataacttaa ctcgtaatg gatctagaa 
        aatgcttca acgactaga ttactcgaa aagattgta 
        gatatttag tgataatgt caattagcg taacattgt 
        aggtaatac tctctatta acattgata tat
      • >TC010230 RC
        gctgtgtaa gaaaattct gcgagattc ttagacgaa 
        atgctccac cgaagcacc tcgtctcgt cgcagacga 
        gcatcacga agtgcagcc tcatctcct caaacccgt 
        ccttctcgc tcggctgga acaagcccc ggtcgtcga 
        gcaagcatt gcagtttca ccagtggac agtgagcga 
        gcctccaga aacaccctc gcacctgaa ataaaccgc 
        agtccacga cacagcatc attccagac atttcccta 
        gattcggaa agtcttcga agccctcga cacagtctc 
        gccccggaa gtcctaagt ccaagaaat agtctagtt 
        ccggacact gcaggcttg agaagtcca agacatagt 
        ctagtgcca aatgacact tactcgcga agtcctcga 
        aacagttta gtgcccgat tctgcgtta agtcctcga 
        aatagttta gtgccggat agttttaat cgaagtccg 
        cgaaatagt ctcgttccg gaagttagt aaaagtggc 
        cgaaatagt ctagtcccg gattcgaat cgtagtcct 
        cgacacagt ttagtacct gatagtaat acctatggt 
        tcgagggca aatttagca ccggatttt aaccgaagt 
        cccagaaat agtcttatt cctgattca agcagaacc 
        ccaagagct agtctcaca ccgtccgag tccacaaca 
        tggcacaaa gatgaagag acggggagc agaagtcca 
        cgtcatagt atacaggtt ccagatagc agtcgaagt 
        cctagagga agtatagct cctgaaagt ctctgcgtt 
        aggacgagt ccgcgtgga agcatagct ggggaatat 
        ccatgcgat cgaagccct cgagggagt attgggcct 
        gaaagtggt ggtgagaac aggagtccg agaggaagc 
        atcgccaga agtacgctg aatgcggag gattgtggt 
        cgaagtccc agaggaagt ctgacgttg actttccag 
        gagccgcct gctaatgaa cgacgctcg agtgcagac 
        aatccttcc ggatcaagg agcggaggt cgtagcaca 
        tctccttat agatcttcc tcaagaggc gcagtcaat 
        gcggcttac agcgggaat tctggattc tcggagagt 
        ggatccaga cgtgccagc agctctgtt agccaggtg 
        tccggggac gagcagcgc cgcctttgc tcctccgac 
        cagaaatac gggggcggc gaacaggga ctggggctc 
        ggtatctca ctcactact tatggatcg gtggcctat 
        caattgaaa gacgccaat ttagaagcg tctggaaca 
        tgcgatttt gtctgcaaa gcattgagc atcgtcaat 
        aaaacagtg gttgttacg atcgttctg gtgtgctta 
        tccactcta cctctacta atgcttatc atgggtatt 
        caattcctg aaagactgc cccagagag ccctacatt 
        cccatctac atggtggtt gggggcagt gtgggctgc 
        gtgaaaatg gggctcacc ctctacaac cagctgcac 
        acccgtcgg atggacgct tccagcaca gctaacgcc 
        gcttccatc ggctcgaga gtcgcttcg atcgccctc 
        accggattc ctgatcatc tggttcagt tttggcaac 
        tactgggtc ttgaggatc aaatggccg gactatgct 
        cccacatta ttcgagccg aaccattgg tgccatcgc 
        actttgtac gtgtttgcg ttggttcat ctctgtgtg 
        gtgtattcg gtgatgggg tgtgtggtg gtgttaatg 
        ttggtgttg gctggatgt caaatgttt gggtgccct 
        tggcttgga ccggcaagg tacaaatag cacaatccg 
        cctgattac ttcgaactg tgggacatg cccaggact 
        taccactga cttgctcaa ggtttgagt tttatcgct 
        tccattcta acaattacg atctcttct tgaataact 
        taactcgta atggatcta gaaaatgct tcaacgact 
        agattactc gaaaagatt gtagatatt tagtgataa 
        tgtcaatta gcgtaacat tgtaggtaa tactctcta 
        ttaacattg atatat
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