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Gene information
General information
TC005603 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10706432..10710529
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005603
    iBeetle screen iB_10983

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature potentially thinner - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature potentially randomnot present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005603 RA
      MLTLYMVVA WLVGICYGF VLISSAAGQ NDVNLFQVG 
      EKAVYKLHS TVLLNEKTG GGKNVGFFI TGDVMVHSL 
      WGESEEKLL KLELKAPQL HIRSRKAPS PDGFIEHSS 
      KLEEFSLKP FYVTWRSGK IGKILLPKG EPKSLANFK 
      KGIASLFQF QILDVETTE NDASGSCKT VYHTIDTTK 
      FRKTKTNCI SSDLPFVKT AHPILDVLV ASSRQVTYE 
      LDPKLTHIK AIYAKESHS MVLSVKEDI GSVIEADQI 
      LNHVKNEKT GPLQGATLE SVVDSVSQG YTEDTLQLQ 
      KEENDDDGK TFPNLVNEL RDNISNLGN LLSAKSFLR 
      LLDAARKAT KDEIVKVLG SKKNQKILP QIYDILGYV 
      QTPEAHASV LKKLHFDKE EHQDLSERY LWSLSLSSH 
      PNPDILKDL LERHRKNDN YPDKVKETL VLTIAQMAF 
      LTQGERKIV RSVEEIIVN NLNYAKGED RFKFLRALK 
      NLKSVTTVP LLVKIIEEG TFKEGVLAF KAIKALGPS 
      RWTPDIFRI ARKTFLQLD RKYDTSART LAADILLEA 
      QPGDSLLKD FLHYLKTND SSVEVKQYV MQRLQMLAD 
      EDRNFNQRL SHLIRSDPK LNNYEVLAP YGLSTALKR 
      TFLRSPSSN GSLLTIQEI FGGIVKRGI VNVMMDKDS 
      QSREMFSLG IFAGGLSGL VGGEVEGDE ETATAGMDL 
      TILDTQIRP FVFFNGQSE LMGHVWSGT ASERTTAFQ 
      ALILLQDHL EFIRLGPGF VAQLSLKGG VSFDLSGNI 
      EISLWGRTA DSNVEKSAG IVTNGLIAV DASFVRSQI 
      EFSASLEPK LNLKSDIDF SGNMNLCMR VTQPDSIFR 
      HNIYKIERI PGSKHKMRI AKYKKVQVP GTTYSLNRK 
      NNEMCSAMF S
    CDS sequence
    • >TC005603 RA
      atgttgact ctgtacatg gtggttgcg tggcttgtg 
      ggaatttgc tacggtttt gtgctgatt tcgtcagcg 
      gcgggccaa aatgacgtg aatttgttc caagtcggg 
      gaaaaagcg gtctataaa ctacactcg accgtgctg 
      ttgaatgaa aaaacgggg ggcggcaaa aacgtgggc 
      tttttcatc acgggggat gttatggtg cacagtctc 
      tggggcgag agcgaggaa aaattactc aaacttgag 
      cttaaggca ccccagttg cacattcgg tcgagaaaa 
      gctccctct cccgatggc ttcatcgaa cattcctcc 
      aaactggag gagttctcc ctaaaaccg ttctatgta 
      acgtggcgc agtggcaaa attggcaag attttgctc 
      ccaaagggc gaacccaag tctttggcc aacttcaaa 
      aaagggata gcgagtcta ttccagttc caaatcctg 
      gatgtggaa accaccgaa aatgacgct tccgggtcg 
      tgcaaaacc gtttaccac accattgac acaacgaag 
      tttcggaaa accaaaacc aattgtatc tcttccgac 
      ttgcccttt gtcaaaacc gcccatcct atcctcgac 
      gtccttgtc gcttcctcc agacaagta acgtacgaa 
      ctggacccc aaactaact cacatcaaa gccatttat 
      gccaaagaa tcacacagc atggtttta tcggtgaag 
      gaagacatt ggcagtgtg atagaagcc gaccaaatc 
      ctgaaccat gtcaaaaat gagaaaact ggacctctc 
      caaggagct acgcttgaa tcagttgtc gattcggtg 
      tctcaaggg tacaccgaa gacaccctt cagttacaa 
      aaagaagaa aatgacgat gatggcaaa acgttccca 
      aacctggtc aacgaactg agggacaac atctcaaat 
      ttgggaaat ttgctttcg gcgaaatca tttttgcgc 
      cttttggac gccgccagg aaggcaacc aaggacgaa 
      attgtcaag gttttgggg tccaagaag aaccaaaag 
      attttgcca caaatttac gatattttg ggctacgtc 
      caaacaccc gaagcccat gccagtgtc ttgaagaag 
      ctccatttc gacaaagag gagcaccag gacttgagc 
      gaacgctac ctgtggtca ctttcactc tcttcgcac 
      cctaacccc gatattttg aaagatttg ttagagaga 
      catcgcaaa aatgacaac tacccggac aaagtcaag 
      gaaacttta gtcctcact attgcacaa atggcgttt 
      ttaacgcag ggcgagcgc aagattgtg cgaagcgtg 
      gaggaaata attgtgaac aacttgaac tatgccaaa 
      ggtgaagac cggttcaag tttctacga gcgctgaag 
      aacctcaaa agtgtcaca accgtcccg ctcctggtc 
      aaaataata gaagaaggg acgttcaaa gagggggta 
      cttgcattc aaagccatt aaggccctc ggtccttct 
      cgatggacc cctgacata ttcagaata gcgcggaaa 
      acgtttttg caactcgac cggaaatac gacacgagt 
      gctcgcacc ctagccgct gatatcctc ctagaagcc 
      caacccggc gacagtctc ttgaaagac ttcttacac 
      tacctcaaa accaacgat tctagtgtc gaagtcaag 
      caatacgtc atgcagcgg ctccaaatg ttggcggac 
      gaggaccga aacttcaac caaagactg tcgcacttg 
      atccgatcc gacccaaag ttgaacaat tacgaagtg 
      ttggcccct tatggcctt tcgaccgct ttaaaacgc 
      acgtttttg cgcagtccc tcaagcaat ggcagtctt 
      ctcacaatc caggaaatc tttggcggg attgttaag 
      cgcggaatc gtcaatgtc atgatggat aaagacagc 
      cagagtagg gaaatgttc agtttgggg attttcgcc 
      ggcggtttg agcggttta gtgggtggc gaagtggaa 
      ggtgacgag gaaacggcc actgctggc atggacctc 
      acaattctg gacacgcaa atccgcccg tttgtcttc 
      ttcaacggt cagagcgaa ctcatgggt catgtgtgg 
      tctggaaca gcgtcggaa cgcaccacg gcgttccag 
      gcccttatt ttgctgcaa gaccacctc gagtttatc 
      agactgggg ccaggcttc gtggcccag ctcagtctg 
      aaagggggc gtgtctttc gacttgtct ggaaacatc 
      gagatttcg ctgtggggc cgcacggct gattccaac 
      gtcgagaaa tcggccggg attgtgacc aatgggttg 
      attgcagtc gatgcgtct ttcgtgagg tcgcagatc 
      gagttcagt gcctcgctt gaaccgaaa ctgaatctc 
      aaatcagac atagacttc tcaggaaac atgaatctg 
      tgtatgagg gtcactcag ccggattcg attttcaga 
      cacaacatt tacaaaatc gaacggatc cccgggagc 
      aagcacaag atgcgaatc gccaagtac aagaaagtg 
      caagtgccg ggaacaacg tactccctg aacaggaag 
      aacaacgaa atgtgtagt gctatgttt agttaa
    mRNA sequence
    • >TC005603 RA
      aaacatccc gtttaagaa attattcca aaacggttt 
      caatgccta atcttccaa ccagcaggt gttccagat 
      tgaaatcac cttgacctt gccttggaa tgcatgaga 
      gtaaaacta cattaacac tgtaacaat tagatgttt 
      cacgacgga attaaaatc attaaacaa tgatgggta 
      acgacgata acgtgcagg agttaacaa gtagaacaa 
      ggttttaac cgtatgctt taacggtat ttactgaat 
      cacgtcgag gaggaggat gtgtaagca aaaattccg 
      aagaggtgg tttggggaa agtaccgca agagaaggg 
      cccaatgct agtccagac ccaaagtcc aggaattac 
      aggaaaaag ctacgtgtt gtgataatg actgtattt 
      ttcaataaa cgactgaga tagtacaca gcatagaaa 
      tccggtttg tcgtagtgc ccacttgcg tattctcct 
      cagagaccc ggtcagtgt gcctccaac tcgcgctaa 
      aatacctca attcgaatc gtccacgct agcggaagt 
      gctagcctt gcaaaatgt gtctgtttt tttcaaaaa 
      tcctgggta taaaatgag ccggcaaca ggcgattga 
      taactggac gataagaat ccacacttt gaccccgag 
      ctgagatag ctccaagaa atgttcagt gacaattac 
      taggtgcga aaatgttga ctctgtaca tggtggttg 
      cgtggcttg tgggaattt gctacggtt ttgtgctga 
      tttcgtcag cggcgggcc aaaatgacg tgaatttgt 
      tccaagtcg gggaaaaag cggtctata aactacact 
      cgaccgtgc tgttgaatg aaaaaacgg ggggcggca 
      aaaacgtgg gctttttca tcacggggg atgttatgg 
      tgcacagtc tctggggcg agagcgagg aaaaattac 
      tcaaacttg agcttaagg caccccagt tgcacattc 
      ggtcgagaa aagctccct ctcccgatg gcttcatcg 
      aacattcct ccaaactgg aggagttct ccctaaaac 
      cgttctatg taacgtggc gcagtggca aaattggca 
      agattttgc tcccaaagg gcgaaccca agtctttgg 
      ccaacttca aaaaaggga tagcgagtc tattccagt 
      tccaaatcc tggatgtgg aaaccaccg aaaatgacg 
      cttccgggt cgtgcaaaa ccgtttacc acaccattg 
      acacaacga agtttcgga aaaccaaaa ccaattgta 
      tctcttccg acttgccct ttgtcaaaa ccgcccatc 
      ctatcctcg acgtccttg tcgcttcct ccagacaag 
      taacgtacg aactggacc ccaaactaa ctcacatca 
      aagccattt atgccaaag aatcacaca gcatggttt 
      tatcggtga aggaagaca ttggcagtg tgatagaag 
      ccgaccaaa tcctgaacc atgtcaaaa atgagaaaa 
      ctggacctc tccaaggag ctacgcttg aatcagttg 
      tcgattcgg tgtctcaag ggtacaccg aagacaccc 
      ttcagttac aaaaagaag aaaatgacg atgatggca 
      aaacgttcc caaacctgg tcaacgaac tgagggaca 
      acatctcaa atttgggaa atttgcttt cggcgaaat 
      catttttgc gccttttgg acgccgcca ggaaggcaa 
      ccaaggacg aaattgtca aggttttgg ggtccaaga 
      agaaccaaa agattttgc cacaaattt acgatattt 
      tgggctacg tccaaacac ccgaagccc atgccagtg 
      tcttgaaga agctccatt tcgacaaag aggagcacc 
      aggacttga gcgaacgct acctgtggt cactttcac 
      tctcttcgc accctaacc ccgatattt tgaaagatt 
      tgttagaga gacatcgca aaaatgaca actacccgg 
      acaaagtca aggaaactt tagtcctca ctattgcac 
      aaatggcgt ttttaacgc agggcgagc gcaagattg 
      tgcgaagcg tggaggaaa taattgtga acaacttga 
      actatgcca aaggtgaag accggttca agtttctac 
      gagcgctga agaacctca aaagtgtca caaccgtcc 
      cgctcctgg tcaaaataa tagaagaag ggacgttca 
      aagaggggg tacttgcat tcaaagcca ttaaggccc 
      tcggtcctt ctcgatgga cccctgaca tattcagaa 
      tagcgcgga aaacgtttt tgcaactcg accggaaat 
      acgacacga gtgctcgca ccctagccg ctgatatcc 
      tcctagaag cccaacccg gcgacagtc tcttgaaag 
      acttcttac actacctca aaaccaacg attctagtg 
      tcgaagtca agcaatacg tcatgcagc ggctccaaa 
      tgttggcgg acgaggacc gaaacttca accaaagac 
      tgtcgcact tgatccgat ccgacccaa agttgaaca 
      attacgaag tgttggccc cttatggcc tttcgaccg 
      ctttaaaac gcacgtttt tgcgcagtc cctcaagca 
      atggcagtc ttctcacaa tccaggaaa tctttggcg 
      ggattgtta agcgcggaa tcgtcaatg tcatgatgg 
      ataaagaca gccagagta gggaaatgt tcagtttgg 
      ggattttcg ccggcggtt tgagcggtt tagtgggtg 
      gcgaagtgg aaggtgacg aggaaacgg ccactgctg 
      gcatggacc tcacaattc tggacacgc aaatccgcc 
      cgtttgtct tcttcaacg gtcagagcg aactcatgg 
      gtcatgtgt ggtctggaa cagcgtcgg aacgcacca 
      cggcgttcc aggccctta ttttgctgc aagaccacc 
      tcgagttta tcagactgg ggccaggct tcgtggccc 
      agctcagtc tgaaagggg gcgtgtctt tcgacttgt 
      ctggaaaca tcgagattt cgctgtggg gccgcacgg 
      ctgattcca acgtcgaga aatcggccg ggattgtga 
      ccaatgggt tgattgcag tcgatgcgt ctttcgtga 
      ggtcgcaga tcgagttca gtgcctcgc ttgaaccga 
      aactgaatc tcaaatcag acatagact tctcaggaa 
      acatgaatc tgtgtatga gggtcactc agccggatt 
      cgattttca gacacaaca tttacaaaa tcgaacgga 
      tccccggga gcaagcaca agatgcgaa tcgccaagt 
      acaagaaag tgcaagtgc cgggaacaa cgtactccc 
      tgaacagga agaacaacg aaatgtgta gtgctatgt 
      ttagttaag tgtaccaag tgctgtgtt aagactcgt 
      gctcaattg taat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY