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Gene information
General information
TC003439 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5177577..5179880
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003439
    iBeetle screen iB_10982

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003439 RA
      MNALIKFIV LLIALAIGN VIYKINTKE SVKVPPETW 
      WGPGDPSKE DTRIVPFKI QVPNQILED LRQRLKNAR 
      KFAPPLEGV HQHYGINTN LLKEIVNYW LTKYDWRER 
      ENFLNQYPQ FKTNIQGLD VHFIHVKPK NVPSGVKTQ 
      PLLLVHGWP GSVREFYEI IPLLTTVQK DKKFVFEVI 
      IPSLPGYGF SQAAVRPGL GAHQTAVIF KNLMKRLGF 
      DRYYVQGGD WGSAVTSAM ALYYPDRVK GIHLNMCVS 
      NSYLAKLKL LAGSVWPSL VVEEKQKHK IYPLSNYFS 
      NALLEFGYM HLQATKPDT IGVALNDSP VGLAAYIIE 
      KFTTWTNPE WKNRADGGL LERFTYDKI LDNIMIYWV 
      TNSITTSMR IYAESINKE SNVFDDRAV ITVPSACAL 
      FDHEIIYQP VSIFKDRFA KLVQVNEYD GGHFAAFEV 
      PESLAKDIW LAVSKFEEP PKPNK
    CDS sequence
    • >TC003439 RA
      atgaacgct ttaataaaa tttattgtt ctattaatc 
      gccttggca ataggtaac gtaatttat aaaataaat 
      actaaggag tcggtaaaa gtgcctccg gaaacgtgg 
      tgggggccg ggagatccc tcaaaagag gacacaagg 
      attgttcct ttcaaaatc caagtgcct aaccagatt 
      ttggaagac ttgcgccaa agactgaaa aatgctcgc 
      aaattcgcg ccccctctc gaaggcgtc caccagcac 
      tacggcata aacacaaac ctcctaaag gaaatagtc 
      aactactgg ctcacgaaa tacgactgg cgagaacgg 
      gagaatttc ctcaaccaa tacccccag ttcaagaca 
      aacattcaa ggcttggac gtccacttc atccacgtt 
      aaacccaaa aacgtgccc agtggagtt aaaacccag 
      cctttgctt cttgtgcac ggctggccc ggttctgtc 
      cgggagttc tacgagata atcccgctc ctcaccacg 
      gtccagaaa gacaagaaa ttcgttttt gaagtcatt 
      attccctcc ttgcccggg tacggattt tcgcaagcg 
      gcagtcaga cccggtcta ggggcgcac caaacggct 
      gtgattttc aagaacttg atgaagcgg ttgggtttt 
      gataggtac tatgtccaa gggggtgat tggggctcg 
      gctgtcacg tctgcaatg gcgttgtat tatcctgat 
      agggttaaa gggattcat ttgaatatg tgtgtctcg 
      aatagctat ttggcgaag ttaaagctt ctagctggg 
      agcgtttgg ccaagtttg gtcgttgaa gagaagcaa 
      aaacataag atataccca ctgtctaat tacttctct 
      aatgcctta ttggaattt ggttatatg catcttcaa 
      gcaacaaaa cctgatact attggtgtt gcacttaat 
      gattctcct gtaggattg gccgcttat attattgaa 
      aaattcacc acttggact aacccggaa tggaaaaat 
      cgcgcagat ggggggttg cttgaaagg ttcacttat 
      gacaaaatt ctggacaat atcatgatt tattgggtg 
      acgaattcc ataacaacg tccatgcga atctacgcg 
      gaatcaatt aacaaagaa agtaatgtt ttcgatgac 
      agggctgtt ataaccgtc ccttcagct tgtgcgctt 
      tttgaccac gaaattatc tatcagccc gtctctatt 
      ttcaaagac agatttgcc aaacttgta caagttaat 
      gaatatgat ggtgggcat tttgcggcc tttgaagta 
      ccagagtcg ttggcaaag gatatatgg ttggctgtt 
      tcaaagttc gaggaacct ccaaagcca aataaatga
    mRNA sequence
    • >TC003439 RA
      gattggcag gaaaactat cattttatc gtgccactc 
      ttcacttga ttcacatgt gagctacct aaacttcca 
      cagataaaa cttgacttt taatgaatc gccatcgcg 
      cgacgtttt tattgatag tatagctag tgataagtg 
      aaataaata catcatcct gtggcaatt aaatctaaa 
      atgaacgct ttaataaaa tttattgtt ctattaatc 
      gccttggca ataggtaac gtaatttat aaaataaat 
      actaaggag tcggtaaaa gtgcctccg gaaacgtgg 
      tgggggccg ggagatccc tcaaaagag gacacaagg 
      attgttcct ttcaaaatc caagtgcct aaccagatt 
      ttggaagac ttgcgccaa agactgaaa aatgctcgc 
      aaattcgcg ccccctctc gaaggcgtc caccagcac 
      tacggcata aacacaaac ctcctaaag gaaatagtc 
      aactactgg ctcacgaaa tacgactgg cgagaacgg 
      gagaatttc ctcaaccaa tacccccag ttcaagaca 
      aacattcaa ggcttggac gtccacttc atccacgtt 
      aaacccaaa aacgtgccc agtggagtt aaaacccag 
      cctttgctt cttgtgcac ggctggccc ggttctgtc 
      cgggagttc tacgagata atcccgctc ctcaccacg 
      gtccagaaa gacaagaaa ttcgttttt gaagtcatt 
      attccctcc ttgcccggg tacggattt tcgcaagcg 
      gcagtcaga cccggtcta ggggcgcac caaacggct 
      gtgattttc aagaacttg atgaagcgg ttgggtttt 
      gataggtac tatgtccaa gggggtgat tggggctcg 
      gctgtcacg tctgcaatg gcgttgtat tatcctgat 
      agggttaaa gggattcat ttgaatatg tgtgtctcg 
      aatagctat ttggcgaag ttaaagctt ctagctggg 
      agcgtttgg ccaagtttg gtcgttgaa gagaagcaa 
      aaacataag atataccca ctgtctaat tacttctct 
      aatgcctta ttggaattt ggttatatg catcttcaa 
      gcaacaaaa cctgatact attggtgtt gcacttaat 
      gattctcct gtaggattg gccgcttat attattgaa 
      aaattcacc acttggact aacccggaa tggaaaaat 
      cgcgcagat ggggggttg cttgaaagg ttcacttat 
      gacaaaatt ctggacaat atcatgatt tattgggtg 
      acgaattcc ataacaacg tccatgcga atctacgcg 
      gaatcaatt aacaaagaa agtaatgtt ttcgatgac 
      agggctgtt ataaccgtc ccttcagct tgtgcgctt 
      tttgaccac gaaattatc tatcagccc gtctctatt 
      ttcaaagac agatttgcc aaacttgta caagttaat 
      gaatatgat ggtgggcat tttgcggcc tttgaagta 
      ccagagtcg ttggcaaag gatatatgg ttggctgtt 
      tcaaagttc gaggaacct ccaaagcca aataaatga 
      tcttgtttt atgatgttt ttgtgattt ttaatttgt 
      tgttgaaaa tgcaaaaat aataaatgt ggtttttta 
      gt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY