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Gene information
General information
TC033267
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013015
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 15148949..15155716
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033267
    iBeetle screen iB_10981

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands content not present - Number of animals showing the phenotype: 2 (13 dpi)
    Days post injection



    abdominal odoriferous glands content darker - Number of animals showing the phenotype: 2 (13 dpi)
    Days post injection



    Fertility
    mature egg not present - Number of animals showing the phenotype: 4

    egg chamber not present - Number of animals showing the phenotype: 4

    tropharium degenerated - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033267 RA
      MSRRARLEE EKQRHLNSE LEEEENRLK AQRERIYAP 
      IIWKRLGSG IRIQDLKPV YYDQVLRII QECYFQEEV 
      LCRNSNLGE DPDSIKSFL NMVLFYLKD RTSIIALDE 
      SNEDAVAGF LVLKAVHKT DFSNVFGRV FHVEGEAHK 
      KCLAFTNYL GRKADVYTD LNCDIFLQF CLLCIRPEY 
      RKKALGLQL FLSAVDVAR SLNIPVVMG ILSCWTLQK 
      LAKRIGMKV KLCKVRQPT IQSCRPFLR LITWAGGTD 
      STR
    • >TC033267 RB
      MEENFVLCS YQPTRLKEM LMSRRARLE EEKQRHLNS 
      ELEEEENRL KAQRERIYA PIIWKRLGS GIRIQDLKP 
      VYYDQVLRI IQECYFQEE VLCRNSNLG EDPDSIKSF 
      LNMVLFYLK DRTSIIALD ESNEDAVAG FLVLKAVHK 
      TDFSNVFGR VFHVEGEAH KKCLAFTNY LGRKADVYT 
      DLNCDIFLQ FCLLCIRPE YRKKALGLQ LFLSAVDVA 
      RSLNIPVVM GILSCWTLQ KLAKRIGMK VKLCKVRQP 
      TIQSCRPFL RLITWAGGT DSTR
    CDS sequence
    • >TC033267 RA
      atgagtcgt cgggcgcgt ctcgaagaa gaaaaacag 
      cgccacttg aactccgaa ctcgaagaa gaggaaaac 
      cgcctgaaa gcccaacgc gaacgcatc tacgcacct 
      ataatatgg aaacggttg ggttccggt atccggatc 
      caagactta aagccggtt tattacgat caagttttg 
      cgaataatc caagaatgc tattttcaa gaggaggtg 
      ctttgtcgg aatagcaac ctcggagag gatccagac 
      tcgataaaa tccttccta aatatggtg ctgttttac 
      ttgaaagat cgcacttcc attatcgcg ctggatgag 
      tccaacgaa gacgctgtc gctggcttc ctggtgctc 
      aaagccgta cacaaaacc gactttagc aacgtcttc 
      gggcgcgtt ttccacgtg gagggcgaa gcccacaaa 
      aagtgcctc gcgttcaca aactacctc ggccgaaaa 
      gccgacgtt tataccgac ctcaactgt gacatattc 
      ctccaattt tgtctgtta tgcatccga ccagaatac 
      cgcaaaaag gctctaggt ctacaacta ttcctctcg 
      gctgtagac gtagcccga tccctgaat atccccgtc 
      gtaatggga atcctgtcc tgttggacc ctccaaaag 
      ctcgcaaaa cgaatcgga atgaaagta aaattatgc 
      aaagtcagg caaccaaca atacaatct tgtagaccg 
      tttttgaga ttgattacg tgggctggc gggacagat 
      tcaacgaga tga
    • >TC033267 RB
      atggaggaa aatttcgtg ttgtgcagc taccagccc 
      acgagattg aaagaaatg ttgatgagt cgtcgggcg 
      cgtctcgaa gaagaaaaa cagcgccac ttgaactcc 
      gaactcgaa gaagaggaa aaccgcctg aaagcccaa 
      cgcgaacgc atctacgca cctataata tggaaacgg 
      ttgggttcc ggtatccgg atccaagac ttaaagccg 
      gtttattac gatcaagtt ttgcgaata atccaagaa 
      tgctatttt caagaggag gtgctttgt cggaatagc 
      aacctcgga gaggatcca gactcgata aaatccttc 
      ctaaatatg gtgctgttt tacttgaaa gatcgcact 
      tccattatc gcgctggat gagtccaac gaagacgct 
      gtcgctggc ttcctggtg ctcaaagcc gtacacaaa 
      accgacttt agcaacgtc ttcgggcgc gttttccac 
      gtggagggc gaagcccac aaaaagtgc ctcgcgttc 
      acaaactac ctcggccga aaagccgac gtttatacc 
      gacctcaac tgtgacata ttcctccaa ttttgtctg 
      ttatgcatc cgaccagaa taccgcaaa aaggctcta 
      ggtctacaa ctattcctc tcggctgta gacgtagcc 
      cgatccctg aatatcccc gtcgtaatg ggaatcctg 
      tcctgttgg accctccaa aagctcgca aaacgaatc 
      ggaatgaaa gtaaaatta tgcaaagtc aggcaacca 
      acaatacaa tcttgtaga ccgtttttg agattgatt 
      acgtgggct ggcgggaca gattcaacg agatga
    mRNA sequence
    • >TC033267 RA
      cctctaaat ttccagtga tgagtcgtc gggcgcgtc 
      tcgaagaag aaaaacagc gccacttga actccgaac 
      tcgaagaag aggaaaacc gcctgaaag cccaacgcg 
      aacgcatct acgcaccta taatatgga aacggttgg 
      gttccggta tccggatcc aagacttaa agccggttt 
      attacgatc aagttttgc gaataatcc aagaatgct 
      attttcaag aggaggtgc tttgtcgga atagcaacc 
      tcggagagg atccagact cgataaaat ccttcctaa 
      atatggtgc tgttttact tgaaagatc gcacttcca 
      ttatcgcgc tggatgagt ccaacgaag acgctgtcg 
      ctggcttcc tggtgctca aagccgtac acaaaaccg 
      actttagca acgtcttcg ggcgcgttt tccacgtgg 
      agggcgaag cccacaaaa agtgcctcg cgttcacaa 
      actacctcg gccgaaaag ccgacgttt ataccgacc 
      tcaactgtg acatattcc tccaatttt gtctgttat 
      gcatccgac cagaatacc gcaaaaagg ctctaggtc 
      tacaactat tcctctcgg ctgtagacg tagcccgat 
      ccctgaata tccccgtcg taatgggaa tcctgtcct 
      gttggaccc tccaaaagc tcgcaaaac gaatcggaa 
      tgaaagtaa aattatgca aagtcaggc aaccaacaa 
      tacaatctt gtagaccgt ttttgagat tgattacgt 
      gggctggcg ggacagatt caacgagat gattttcga 
      cgatcccgg aattgggaa ttacacgtg tgccgtaat 
      ggcggggcc ggtgccgcc gcccccgga ggtggaacc 
      gcctccgaa ggtggacga ag
    • >TC033267 RB
      aatcgctgt cgtgttcca aatcgtaat taaagagcg 
      cgcaattag gcgagtcct cggtgagaa attgaggta 
      attcggatt tgtcctccg ttcgttttg agtcttaat 
      ttcctggca gaagcgctt tccgataag atagccgtt 
      cttccggta acgctttcg tttgagttg catccggtt 
      tacgtgctg gcaacaatt ctcgtaggt cgatatttt 
      gatagcagt gaccccgcg ataagcctc cctcccgtt 
      ggaaaaaac ggcatttcc gccccttgt ttctcgtca 
      tgccatatt agagcatca aattcggag ttgttcgat 
      tgcctttta cggccggat tcctgctgg accagtccg 
      ccatagcgc agttttggg ctagctttg tcccgtttt 
      ggtaaaaga tttttcaga gacgcgttc gttgctaat 
      aaggtttgg cgccgacgg tgcaactac ctcatgaat 
      aaccagccc agaacgcga ttagatgtt tacaaaagg 
      agcacgagg agcgtttga ttcattcca ggattccag 
      cgacaaatg acaacgttt ttactgccg agtaagtca 
      cataaactg caacattat catcaggtg agcaaactt 
      gaagcgcta aaaatagac ggggactga aattttgta 
      aacaaatgc ttcaagtgc aaggagata agtgctcgg 
      gagccaacc acttgaata aaaagttac acagacttg 
      atgtgatca aagagagcg agggaaggg gtgccctga 
      tttgtattc cacccacag ctgggtttt tggataatt 
      cgcagaatt ggatggatg gatgatggg ttttcgaat 
      cgaactcgc ctgcaatta tcgcccaaa ttgggttcc 
      cgtttgcac cacggcttt ctcacactg tggtgggct 
      tttttacgc ccacgatgg ttctttccc acacaacca 
      cgtttgaag ggcgacaac tggtcgata aactttttt 
      tacaacttg tgttaacaa atttttccc actctcact 
      gttgcaatt atgtaaata cactcaacc acccccagc 
      gaaataaaa ttgcacctc ttaacattc ttaaattcc 
      gtgcttcga gcccaacaa aacacctcg agcccgaca 
      attcaacaa attacactc tttaccaag cactttgcc 
      tgcgttctg tcgtcacgc ctcttccaa ctactgttt 
      aagcccctt tttaacccc aaccaaacg tttaaaatt 
      cctgcggac cccactttt aatcattaa agtgaatcg 
      gattgctcg cgtgaccgc gcgacatat tttaacgac 
      ttgccacca ataagcgaa attaaatag tttgacgat 
      tttaaagaa gtggccaac tgtttaatc agtccctaa 
      ttaatgaag gatttttcg ggatttatc gacccggcc 
      ggcggacgg tttttcctc cttccgggg tcggggaaa 
      ttgcattga aacctgttc gtaaataag aggggcggc 
      tgcccgtac tcaaatttg ttctgacta cgtaaacac 
      cattgttgg aggcggcct gggggcggt tgcctcaat 
      tcgctactt aaattaatt acgttctga tcgacaact 
      gaccttagt gtaagtgct cgatccctc caaacaata 
      cataaaaaa atccactag aaactctcc tatccgtta 
      aagaaaaaa agaaccaaa acacaagac tacactact 
      ctcgaacac ctactcccg ctccagcat ggcgcctcg 
      cgcaaactg attttctca acattctca gactgttat 
      gcaccagtc ccagccaga gaacgcatg cgttcggaa 
      ttcaatact cattcgatt tgaatcctc atgcattca 
      aagggctgc caccaagac actgaaaga ctttttaac 
      gagagacat gtgtgccgg gtctgactt tttaattac 
      ttgcgtcaa gacatcagt tttttgact tgggaaata 
      tgagggacg tctcacact tggagataa agatactcc 
      attttcgca atggtttag tcaatacaa aacatatct 
      gtttgtgtt tcaaaacca ttaagtaac aaaaatttc 
      aagtgctct tgatgtcag acattcaga tggaggaaa 
      atttcgtgt tgtgcagct accagccca cgagattga 
      aagaaatgt tgatgagtc gtcgggcgc gtctcgaag 
      aagaaaaac agcgccact tgaactccg aactcgaag 
      aagaggaaa accgcctga aagcccaac gcgaacgca 
      tctacgcac ctataatat ggaaacggt tgggttccg 
      gtatccgga tccaagact taaagccgg tttattacg 
      atcaagttt tgcgaataa tccaagaat gctattttc 
      aagaggagg tgctttgtc ggaatagca acctcggag 
      aggatccag actcgataa aatccttcc taaatatgg 
      tgctgtttt acttgaaag atcgcactt ccattatcg 
      cgctggatg agtccaacg aagacgctg tcgctggct 
      tcctggtgc tcaaagccg tacacaaaa ccgacttta 
      gcaacgtct tcgggcgcg ttttccacg tggagggcg 
      aagcccaca aaaagtgcc tcgcgttca caaactacc 
      tcggccgaa aagccgacg tttataccg acctcaact 
      gtgacatat tcctccaat tttgtctgt tatgcatcc 
      gaccagaat accgcaaaa aggctctag gtctacaac 
      tattcctct cggctgtag acgtagccc gatccctga 
      atatccccg tcgtaatgg gaatcctgt cctgttgga 
      ccctccaaa agctcgcaa aacgaatcg gaatgaaag 
      taaaattat gcaaagtca ggcaaccaa caatacaat 
      cttgtagac cgtttttga gattgatta cgtgggctg 
      gcgggacag attcaacga gatgatttt cgacgatcc 
      cggaattgg gaattacac gtgtgccgt aatggcggg 
      gccggtgcc gccgccccc ggaggtgga accgcctcc 
      gaaggtgga cgaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY