Status
Please wait ...
Gene information
General information
TC005824 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7161082..7163889
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC005824
      iBeetle screen iB_10980

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC005824 RA
        MSQSSAVYQ PTTVTYEAN GPQERWQDR 
        PISYLASMS QAARRPCGL TCLPSRNIL 
        PVVLITSSV ASFSLGLVM LIHGAMGYS 
        DTPEDKEDL YLIITIFGA IFFALSILL 
        FVFFMRLTR RICWRNAKD HLNASGGQA 
        LTVNPSTDF LVSAQYAPV SEVAYQPAQ 
        EDTEQSKLM PQENKEIED TDRMVESDP 
        RIVLRPLNP SAHEET
      CDS sequence
      • >TC005824 RA
        atgtcgcaa agcagcgcc gtttaccag cccaccacc 
        gtcacgtac gaggccaat ggcccccag gagcggtgg 
        caggaccgc cccatcagc tacctggcc tccatgagc 
        caggctgcc aggcgcccc tgcggcctc acctgcctg 
        ccctcccgg aacatcctc cccgtggtg ctcatcacc 
        tcaagtgtg gcctcgttc tcgctcggt ctcgttatg 
        ctcatacac ggcgccatg gggtactcg gacaccccc 
        gaagacaag gaggatctc tatctcata atcacaatc 
        tttggagca atatttttc gcgctatcg atacttcta 
        tttgtgttt ttcatgcgg ctcacgcgc cggatttgt 
        tggaggaac gccaaagac cacttgaat gcctcggga 
        ggacaagcc cttacagtt aatccgtct accgatttt 
        ctcgtttcg gctcaatac gcccctgtg tccgaagtg 
        gcctaccag ccggcgcag gaggacacc gagcaaagt 
        aaattaatg ccgcaggaa aataaagag attgaagat 
        acggaccgt atggttgag agcgacccc cgtattgtt 
        ttacgtcct ttaaaccca tccgcccat gaagagact 
        taa
      mRNA sequence
      • >TC005824 RA
        cgaaaaatg tgataaaac taaattaag tcgtagtat 
        ggttatttt tagcgttat taatattca tgatgttgt 
        gaccttgac gacaattat gctattttc catcccagc 
        gtaattttt aatctgaaa ttagatcac taacacaag 
        gccaagggc acttgaaaa tgaaaattc aaatgcccc 
        acatgaact aaacagagt gtcaaatgt ttttcccga 
        gcactctcg aaagcgttc attagtttc tctttttgc 
        ggtgactca ccggtcagc ggcgtagaa attggtcgc 
        aattattaa ttcaagtct aaatactct aaaaaacct 
        tgctaggtg tttttcgcg ccaaaaaaa ttgagaaat 
        tgttagttt ttccataat ttatggtga ttgacgggt 
        caatgacag cagccgcct gcgataaac ccgccgcaa 
        ttcccttgc cccttgcgc acgtgacgc cgctggttt 
        cacctgcgg aagcaccaa ttgtgtcga gtccacctt 
        ttcagccgc tcgaacctt gaatatgaa atagaaaaa 
        cccgtttcg gggtgtttt tctctcgaa aaaatccgg 
        aaaagtcgc gccagtgtc cccacattt gacatctgt 
        caaagtgca tcgaatcgt acgaatgaa acggcccgc 
        caaagtggg aatttaacg aaatgtcgc aaagcagcg 
        ccgtttacc agcccacca ccgtcacgt acgaggcca 
        atggccccc aggagcggt ggcaggacc gccccatca 
        gctacctgg cctccatga gccaggctg ccaggcgcc 
        cctgcggcc tcacctgcc tgccctccc ggaacatcc 
        tccccgtgg tgctcatca cctcaagtg tggcctcgt 
        tctcgctcg gtctcgtta tgctcatac acggcgcca 
        tggggtact cggacaccc ccgaagaca aggaggatc 
        tctatctca taatcacaa tctttggag caatatttt 
        tcgcgctat cgatacttc tatttgtgt ttttcatgc 
        ggctcacgc gccggattt gttggagga acgccaaag 
        accacttga atgcctcgg gaggacaag cccttacag 
        ttaatccgt ctaccgatt ttctcgttt cggctcaat 
        acgcccctg tgtccgaag tggcctacc agccggcgc 
        aggaggaca ccgagcaaa gtaaattaa tgccgcagg 
        aaaataaag agattgaag atacggacc gtatggttg 
        agagcgacc cccgtattg ttttacgtc ctttaaacc 
        catccgccc atgaagaga cttaagtca cgtccacga 
        aaattttac atttgtata gaatgtatt tttatctca 
        ttttttagt tttatacac actatcacc gcttctttt 
        ccacttgat taactgtga cgagaaacg aatgcttcc 
        gcttaaaat aatttattt tcggtaaaa cccaaccaa 
        gaaaatcgt ataaatatt cgcactgtg tgttatcgt 
        aggaaaaaa attaaaaaa ttacgctgg ttcgtaata 
        ctagatctg aaatattcc gtacttaaa agacatgac 
        gact
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY